MD12G1119100.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003978
Physical Location & Seq
Forward (+)
19084312 .. 19085181
870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1119100.v1.1.491

Sequence Viewer

Length: 573 bp
ATGTGCATTGTTATATGTAGGGGGCATATAAACCCTGCAATCACATTCGGGCTGTTTTTGGTAAGGAGAGTATCACTGGTGAGAGCCATATTGTACATGGCGGCTCAGTCGTTTGGAGCCATATATGGTGTTGGGCTAGTGAAAGCTTTCCACAGTTCTTACTATACCAAGTATGGCGGAGCCAATGAGCTTGCCGATGGCTACAACATAGGCACTGGATTGGGTACTGAGATTCTTGGCACCTTTGTTCTTGTCTACACTGTCTTCTCTGCCACTGATCCCAAGAGAAATGCAAGAGACTCCCATGTCCCAGTATTGGCACCCCTGCCAATTGGGTTTGCTGTGTTTATTGTTCACCTTGCCACAATCCCAATCACTGGCACTGGCATCAACCCAGCTAGAAGTCTTGGAGCTGCAGTGATCTACAACAATGACAAGGCTTGGAATGACCAATGGATCTTCTGGGTTGGACCATTTATTGGAGCTGCCATTGCTGCCTTATATCACCAATACATCTGGAGAGCAGGAGCTGTTAAAGCTCTGGGTTCTTTCAGGAGCTCCCCCAACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

191

Amino Acids

20.5

Weight (kDa)

9.61

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 5 - 169 2.1e-58 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AccB1I GGYRCC 2 cut(s) 239, 319
AccB7I CCANNNNNTGG 2 cut(s) 377, 479
AccI GTMKAC 1 cut(s) 255
AciI CCGC 2 cut(s) 101, 177
AclWI GGATC 2 cut(s) 272, 464
AfaI GTAC 2 cut(s) 95, 226
AfiI CCNNNNNNNGG 3 cut(s) 316, 377, 479
AluBI AGCT 8 cut(s) 146, 190, 398, 413, 485, 530, 539, 558
AluI AGCT 8 cut(s) 146, 190, 398, 413, 485, 530, 539, 558
Alw21I GWGCWC 1 cut(s) 560
Alw26I GTCTC 1 cut(s) 291
AlwI GGATC 2 cut(s) 272, 464
AlwNI CAGNNNCTG 1 cut(s) 530
ApeKI GCWGC 3 cut(s) 413, 485, 494
Asp700I GAANNNNTTC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 3 cut(s) 91, 347, 497
AvaII GGWCC 1 cut(s) 470
BanI GGYRCC 2 cut(s) 239, 319
BanII GRGCYC 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 560
BbvI GCAGC 3 cut(s) 400, 472, 481
BccI CCATC 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 291
BfaI CTAG 2 cut(s) 137, 399
BfmI CTRYAG 1 cut(s) 414
BisI GCNGC 4 cut(s) 102, 414, 486, 495
BlsI GCNGC 4 cut(s) 103, 415, 487, 496
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 4 cut(s) 118, 181, 241, 321
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 1 cut(s) 396
BmuI ACTGGG 1 cut(s) 305
BpiI GAAGAC 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 538
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 377, 479
Bse1I ACTGG 5 cut(s) 81, 220, 311, 382, 388
Bse3DI GCAATG 1 cut(s) 489
BseLI CCNNNNNNNGG 3 cut(s) 316, 377, 479
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 2 cut(s) 119, 219
BseNI ACTGG 5 cut(s) 81, 220, 311, 382, 388
BseXI GCAGC 3 cut(s) 400, 472, 481
BseYI CCCAGC 1 cut(s) 394
BshNI GGYRCC 2 cut(s) 239, 319
BsiHKAI GWGCWC 1 cut(s) 560
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 3 cut(s) 316, 377, 479
BsmAI GTCTC 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 560
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 3 cut(s) 277, 420, 456
BspACI CCGC 2 cut(s) 101, 177
BspCNI CTCAG 2 cut(s) 118, 220
BspLI GGNNCC 4 cut(s) 118, 181, 241, 321
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 2 cut(s) 272, 464
BspT107I GGYRCC 2 cut(s) 239, 319
BsrDI GCAATG 1 cut(s) 489
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 5 cut(s) 81, 220, 311, 382, 388
BssMI GATC 3 cut(s) 277, 420, 456
Bst4CI ACNGT 2 cut(s) 155, 262
BstAUI TGTACA 1 cut(s) 93
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 2 cut(s) 105, 228
BstKTI GATC 3 cut(s) 280, 423, 459
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 3 cut(s) 277, 420, 456
BstMWI GCNNNNNNNGC 3 cut(s) 491, 494, 536
BstSFI CTRYAG 1 cut(s) 414
BstV1I GCAGC 3 cut(s) 400, 472, 481
BstV2I GAAGAC 1 cut(s) 256
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 7 cut(s) 74, 213, 258, 273, 375, 381, 423
Cac8I GCNNGC 1 cut(s) 192
CaiI CAGNNNCTG 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 94, 225
CviAII CATG 2 cut(s) 97, 305
CviQI GTAC 2 cut(s) 94, 225
DdeI CTNAG 2 cut(s) 105, 228
DpnI GATC 3 cut(s) 279, 422, 458
DpnII GATC 3 cut(s) 277, 420, 456
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EciI GGCGGA 1 cut(s) 192
Ecl136II GAGCTC 1 cut(s) 558
Eco24I GRGCYC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 558
EcoICRI GAGCTC 1 cut(s) 558
EcoT38I GRGCYC 1 cut(s) 560
FaeI CATG 2 cut(s) 100, 308
FaqI GGGAC 1 cut(s) 293
FatI CATG 2 cut(s) 96, 304
FauNDI CATATG 1 cut(s) 569
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 4 cut(s) 102, 414, 486, 495
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 4 cut(s) 102, 414, 486, 495
FspBI CTAG 2 cut(s) 137, 399
GluI GCNGC 4 cut(s) 102, 414, 486, 495
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 538
Hin1II CATG 2 cut(s) 100, 308
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 2 cut(s) 232, 299
HphI GGTGA 3 cut(s) 91, 347, 497
Hpy166II GTNNAC 2 cut(s) 256, 355
Hpy188III TCNNGA 2 cut(s) 517, 553
Hpy8I GTNNAC 2 cut(s) 256, 355
HpyCH4III ACNGT 2 cut(s) 155, 262
HpyCH4V TGCA 4 cut(s) 6, 38, 293, 416
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 494, 536
HpyF3I CTNAG 2 cut(s) 105, 228
Hsp92II CATG 2 cut(s) 100, 308
Kzo9I GATC 3 cut(s) 277, 420, 456
LmnI GCTCC 7 cut(s) 116, 179, 410, 482, 527, 555, 563
Lsp1109I GCAGC 3 cut(s) 400, 472, 481
LweI GCATC 1 cut(s) 396
MaeI CTAG 2 cut(s) 137, 399
MalI GATC 3 cut(s) 279, 422, 458
MboI GATC 3 cut(s) 277, 420, 456
MboII GAAGA 2 cut(s) 256, 451
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 1 cut(s) 560
MluCI AATT 1 cut(s) 330
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 448
MroXI GAANNNNTTC 1 cut(s) 146
MseI TTAA 1 cut(s) 534
MunI CAATTG 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 491, 494, 536
NdeI CATATG 1 cut(s) 569
NdeII GATC 3 cut(s) 277, 420, 456
NlaIII CATG 2 cut(s) 100, 308
NlaIV GGNNCC 4 cut(s) 118, 181, 241, 321
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 1 cut(s) 232
PflMI CCANNNNNTGG 2 cut(s) 377, 479
PkrI GCNGC 4 cut(s) 103, 415, 487, 496
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 394
PspN4I GGNNCC 4 cut(s) 118, 181, 241, 321
PspPI GGNCC 1 cut(s) 470
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 456
RsaI GTAC 2 cut(s) 95, 226
RsaNI GTAC 2 cut(s) 94, 225
SacI GAGCTC 1 cut(s) 560
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 4 cut(s) 102, 414, 486, 495
Sau3AI GATC 3 cut(s) 277, 420, 456
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 1 cut(s) 293
SduI GDGCHC 1 cut(s) 560
SfaNI GCATC 1 cut(s) 396
SfcI CTRYAG 1 cut(s) 414
SinI GGWCC 1 cut(s) 470
Sse9I AATT 1 cut(s) 330
SsiI CCGC 2 cut(s) 101, 177
SspMI CTAG 2 cut(s) 137, 399
SstI GAGCTC 1 cut(s) 560
TaaI ACNGT 2 cut(s) 155, 262
TasI AATT 1 cut(s) 330
TatI WGTACW 1 cut(s) 93
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 7 cut(s) 81, 220, 265, 280, 382, 388, 423
TseI GCWGC 3 cut(s) 413, 485, 494
TspRI CASTG 7 cut(s) 81, 220, 265, 280, 382, 388, 423
Van91I CCANNNNNTGG 2 cut(s) 377, 479
VpaK11BI GGWCC 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 146
XspI CTAG 2 cut(s) 137, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.