Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0007077

Physical Location & Seq

Genomic Coordinates Reverse (-)

14338348 .. 14342629

Gene Structure 4282 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g20810.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3114 bp

ATGCGTGGCTTAGCCACCAAATTACAGTCCTTGTGTTTGAGCTCCACCAGTAAAATCTTTCGTTTTCGATTGTCAAGAGTTCATAGGATTGATAACTTTGCAACGCTCACTAAACTATCTAAGCCCAAGAAAGCCCAGTTGAAACAAGTAGAAGAAGCAAGCTCGTTTGGATTGCTGTTCAGTGAAATAACTCAGATTTTAGGGGCTGAAAATGTCACTGCAGATAAAACCCCATCTGGGTTTTTCATTTCCAAGGATACCTATGTTGGGGTTGGTGAGGTTTTGAAAGAGAGGGCACCTTGTTTGCCGAGTGTTAAAACTGCTGAGGATGATGCCCTGAGAGTGAAGGAAGATGTAGCTGTTGTGGAGGAAACCAGTGTAGCTGAAGCTGATGTTAGCCCAGTAGTTCATGAGGTTACGAGGATTGTCAGGGCGGAAAGTGGTTTGGTTTCAATGGAGGAGCGGTTAGATGAGTTGCGTTTTGAGTTGGATTCGGAGATTGTTGACAACGTTTTGAAGAGGTGCTTTAAAGTGCCACATTTGGCTTTGAGGTTCTTTGATTGGGTGAAGATGAGAGAAGGGTTCTGTCACACAACTAAGATATATAATACTGTGCTGTGTATAGCTGGGGATGTGAAGGAGTTTGGGATGGTGGAGAAGTTGGTGGAGGAGATGGAAAGGAACTCGTGCAAGAAAGATGTTAAGACTTGGACTATTCTTATCCAGATGTATGGGAAGGCTAAGTTAGTTGGAAGAGCCTTGTTAGTCTATGAAAAGATGAAGAAATTTGGGTTTGAACCAGATGCAGTGGTTTTCAGAATAATGATAACTGCACTCTGTGCTGCTGGAAAAGCTGATGTTGCAATGGAATTTTACAAGGAAATGGTACAGAAGGACATTGGTCTTGATATCAAGATGTATAAGCTGTTACTGAACAGCATAGCTAGGTATGAAGATACTGCTGCTGCTGTGGGCTTGGTTTCAGATAACATGATAAGAGTCTCACAGATTCCTGAACATGTGGTTTATGGTTGTGTACTGAAGTCTTTCTGCATCTCTGGTAGAATCAGAGAAGCTTTGGGATATATTCGTGAACTCAAGAATAAAGAGTTTATGCTTCTCCCTGAGTATTTTGAGACTTTAGTGAAAGGACTGTGCAGGGCTGATAGAATTGCAGATGCTTTGGAGATTTTGGATATTATGAAGAAAAGAGATTTTGTCGATGGAAATGTGTATGGGATTATCATCAATGGGTACTTAAGAAAGAATGATGTTTCTAGAGCACTTGATCTGTTTCATGGTATGAAAGAATCTGGATATTTGCCTACTACTTCTACCTACACGGAACTGATGCAGCACCTCTTCAATATGAATGAGCACCAAAAAGGCTGTGAGCTCTATGATGAGATGCTCGAAAGAGGAGTTGAGCCAGATAGCGTGGCAGTGACAGCCATAGTTGCAGGCCATGTCCGGCAAAATCATATATCTGAAGCATGGAAAGTATTTATACATATGAAGGACAAAGGCATTAAGCCCACTTCGAAGTCATATATGGTATTTATCAAGGAGCTTTGTAAAATTTCAAGGACAGATGAGATTCTCAAGGTCTTGGTTCATATGCAAGCATCTGATATGCGCATTCAAGATGAGATATTCAACTGGGTTATACTTCATATGAAGAAAAAGGGAGAGATGGATAGCCTTGAAAAAGTAAAGCAGATGCGAAGTGTCAACAAATTTCAGCCTCATGATGTAGGGGTATCAAGCAATGATGCATCTACGGGAGAGAGGATCAAGACAGTGTTGGACTCGGTACCAAAGAAGTTGGATCCCTTATCGAACTTTAATGCTTATGATGACAAGGATTTGCAAGAAATTTGTAAGATTCTGTCCTCTTCAACTGACTGGTACTTAGTTCAGGAGGCTTTGGAGAAGTCTGCTGTTGTTTTCACTCCAGGACTCGTTCTGTCAATTTTGAAGAGTCATAGTGCTCATGGTTTGGTAGCATTACAGTTTTTCTCATGGATTGGAAAGCAATCCGGTTATCGCCACACTACTGATACTTACAACATGGCCATCAAAATTGCAGGACGTGGAAAAGATTTCAAGCACATGAGGAACCTTTTGTATGAAATGAGGAGAAACAGATTCCCAATAACACAAGATACATGGACAATAATGATAATGCAATATGGTCGAACAGGCCTCACAGAGATTGCTCTCCAGTTGTTCAGAGAGATGAAAAGCAATTGCAACCCAACTGGCAGTACATACAAGTATCTGATCATATCTCTTTGTGGGAGAAAAGGTCGAAAGGTAGATGAAGCAATTAAAACATTCCAGGAGATGATCCGTGCTGCACATATACCTGACAAAGAGTTAGTTGAAACTTACATTGGTTGTTTATGTGAAGTTGATAAGCTGTCAGATGCTAGAAGATGCATAGATTCTCTTTCGAAAGTTGGTTTTACAACTTCTCTTAGTTATTCCTTGTACATAAGGGCTCTTTGTCGAGCTGGGAGATTACAAGAAGCTTCTGAGATGATGGATGATATTGGGGAAGAGCGATCCACATTGGATCGGTATACATATGGGAGCTTCGTTCATGGACTTCTGCGGAGTGGACAAGTGGAAGAAGCACTTGCCAAGATGGAGTCTATGAAGCAGGCTGGCATGAAGCCAACTGTCCATGTTTATACATCCTTAATAGTTCATTTCTTGAAGGAGAAACAGATCAAAAGGGCATTAGAAATTTTCAATGAAATGCAACAGGAAGGATGTGAACCAACTGTTGTCACTTATTCCACCCTTATTCGGGGGTACATGAATATGGAGATGACTGCAGAAGCTTGGAGTGTCTTTCACAACATGAAACTGAAAGGGCCTTCTCCGGATTTCAAAACTTATTCAATGTTCCTTCATTGTCTATGTAAGGCGGGGAAATCAGAAGAAGCCATGCAGCTTATAACTGAAATGTTGAACAGTGGGATTATCCCCAGTGTTGTCAATTTTCGAACTGTATTTTACGGACTTAACCGAGAAGGTAAGCATGATTTGGCTCGCACTGTTATGGAACAGAAATCATCTTCAATTAGAAAACTGGTAAAGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1038

Amino Acids

118.08

Weight (kDa)

8.61

Isoelectric Point (pI)

26.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	199 - 245	9.4e-06	PPR repeat family
PPR_2	PF13041	232 - 280	1.3e-11	PPR repeat family
PPR_1	PF12854	264 - 296	4.7e-07	PPR repeat
PPR_2	PF13041	267 - 315	2.8e-06	PPR repeat family
PPR_2	PF13041	412 - 451	2.5e-08	PPR repeat family
PPR_long	PF17177	420 - 550	1.8e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	442 - 484	3.8e-08	PPR repeat family
PPR_3	PF13812	466 - 522	8.9e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	477 - 526	2.4e-12	PPR repeat family
PPR_2	PF13041	723 - 766	4.7e-07	PPR repeat family
PPR	PF01535	723 - 750	6.6e-06	PPR repeat
TPR_24	PF23276	838 - 956	2.1e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	862 - 903	1.1e-06	PPR repeat family
PPR_long	PF17177	873 - 1026	1.5e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	885 - 941	1.9e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	894 - 942	1.2e-15	PPR repeat family
TPR_24	PF23276	897 - 988	2.3e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	926 - 958	2.4e-06	PPR repeat
PPR_2	PF13041	931 - 978	3e-13	PPR repeat family
PPR_1	PF12854	960 - 992	2.4e-07	PPR repeat
PPR_2	PF13041	972 - 1011	7.6e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06400
fragaria_vesca	FvH4_6g20810 FvH4_6g20810 FvH4_6g20810
malus_domestica	MD12G1044100.v1.1
prunus_persica	Prupe.7G091000_v2.0.a1
pyrus_communis	pycom12g03800
rosa_chinensis	RchiOBHm_Chr3g0476881
rosa_laevigata	RLG00000023746
rosa_multiflora	Rmu_sc0000288.1_g000033
rosa_roxburghii	Rroxscaffold_6G00403680 Rroxscaffold_6G00404930
rosa_rugosa	Rorug03G0157400
rosa_samantha	Rh3AG207600 Rh3AG229900 Rh3BG240200 Rh3BG241300 Rh3CG233900 Rh3CG235000 Rh3CG235700 Rh3CG259900 Rh3DG233900
rosa_wichuraiana	Rw0G011140 Rw3G018900 Rw3G020670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2966
Acc16I	TGCGCA	1 cut(s)	1637
Acc65I	GGTACC	1 cut(s)	1813
AccB1I	GGYRCC	2 cut(s)	295, 1813
AccBSI	CCGCTC	1 cut(s)	463
AccI	GTMKAC	1 cut(s)	2585
AccIII	TCCGGA	1 cut(s)	2890
AciI	CCGC	4 cut(s)	434, 463, 2617, 2936
AclI	AACGTT	1 cut(s)	510
AclWI	GGATC	6 cut(s)	1799, 1823, 1836, 2344, 2562, 2586
AcoI	YGGCCR	1 cut(s)	2073
AcsI	RAATTY	6 cut(s)	785, 869, 1578, 1736, 1875, 2751
AcuI	CTGAAG	3 cut(s)	405, 1061, 1509
AdeI	CACNNNGTG	1 cut(s)	839
AfaI	GTAC	8 cut(s)	888, 1038, 1256, 1815, 1910, 2269, 2495, 2822
AfiI	CCNNNNNNNGG	4 cut(s)	237, 267, 2814, 2815
AflII	CTTAAG	1 cut(s)	1258
AflIII	ACRYGT	1 cut(s)	1018
AjiI	CACGTC	1 cut(s)	2093
AjnI	CCWGG	2 cut(s)	1954, 2340
AjuI	GAANNNNNNNTTGG	2 cut(s)	2379, 2411
Alw21I	GWGCWC	5 cut(s)	44, 1285, 1380, 1398, 1993
Alw26I	GTCTC	2 cut(s)	1006, 1130
AlwI	GGATC	6 cut(s)	1799, 1823, 1836, 2344, 2562, 2586
Aor13HI	TCCGGA	1 cut(s)	2890
AoxI	GGCC	4 cut(s)	1462, 2073, 2203, 2882
ApeKI	GCWGC	6 cut(s)	842, 962, 965, 1354, 2357, 2959
ApoI	RAATTY	6 cut(s)	785, 869, 1578, 1736, 1875, 2751
Asp700I	GAANNNNTTC	1 cut(s)	1046
Asp718I	GGTACC	1 cut(s)	1813
AspLEI	GCGC	1 cut(s)	1638
AspS9I	GGNCC	1 cut(s)	2882
AsuHPI	GGTGA	2 cut(s)	287, 577
AsuII	TTCGAA	3 cut(s)	1541, 2456, 3013
BaeGI	GKGCMC	1 cut(s)	298
BaeI	ACNNNNGTAYC	2 cut(s)	249, 282
BalI	TGGCCA	1 cut(s)	2075
BamHI	GGATCC	1 cut(s)	1828
BanI	GGYRCC	2 cut(s)	295, 1813
BanII	GRGCYC	3 cut(s)	44, 1398, 2506
BauI	CACGAG	1 cut(s)	685
Bbv12I	GWGCWC	5 cut(s)	44, 1285, 1380, 1398, 1993
BbvCI	CCTCAGC	1 cut(s)	324
BbvI	GCAGC	6 cut(s)	829, 949, 952, 1366, 2344, 2971
BccI	CCATC	8 cut(s)	241, 643, 667, 1217, 1687, 2084, 2539, 2644
BcgI	CGANNNNNNTGC	2 cut(s)	2177, 2211
BciT130I	CCWGG	2 cut(s)	1956, 2342
BciVI	GTATCC	1 cut(s)	250
BclI	TGATCA	1 cut(s)	2283
BcoDI	GTCTC	2 cut(s)	1006, 1130
BfaI	CTAG	3 cut(s)	945, 1278, 2433
BfmI	CTRYAG	2 cut(s)	219, 2841
BfrI	CTTAAG	1 cut(s)	1258
BfuI	GTATCC	1 cut(s)	250
BisI	GCNGC	6 cut(s)	843, 963, 966, 1355, 2358, 2960
BlpI	GCTNAGC	1 cut(s)	10
BlsI	GCNGC	6 cut(s)	844, 964, 967, 1356, 2359, 2961
Bme1390I	CCNGG	2 cut(s)	1956, 2342
BmgBI	CACGTC	1 cut(s)	2093
BmgT120I	GGNCC	1 cut(s)	2882
BmiI	GGNNCC	4 cut(s)	297, 1815, 1830, 2120
BmrFI	CCNGG	2 cut(s)	1956, 2342
BmrI	ACTGGG	4 cut(s)	130, 395, 1669, 2991
BmuI	ACTGGG	4 cut(s)	130, 395, 1669, 2991
BoxI	GACNNNNGTC	1 cut(s)	900
BpmI	CTGGAG	2 cut(s)	1938, 2207
Bpu10I	CCTNAGC	1 cut(s)	324
Bpu1102I	GCTNAGC	1 cut(s)	10
Bpu14I	TTCGAA	3 cut(s)	1541, 2456, 3013
BpuEI	CTTGAG	2 cut(s)	1082, 1586
BsaBI	GATNNNNATC	1 cut(s)	1244
BsaJI	CCNNGG	1 cut(s)	252
BsaWI	WCCGGW	2 cut(s)	2039, 2890
BsaXI	ACNNNNNCTCC	4 cut(s)	449, 479, 2293, 2323
Bsc4I	CCNNNNNNNGG	4 cut(s)	237, 267, 2814, 2815
Bse3DI	GCAATG	2 cut(s)	870, 1774
Bse8I	GATNNNNATC	1 cut(s)	1244
BseAI	TCCGGA	1 cut(s)	2890
BseBI	CCWGG	2 cut(s)	1956, 2342
BseDI	CCNNGG	1 cut(s)	252
BseGI	GGATG	6 cut(s)	334, 637, 654, 2554, 2699, 2783
BseJI	GATNNNNATC	1 cut(s)	1244
BseLI	CCNNNNNNNGG	4 cut(s)	237, 267, 2814, 2815
BseMI	GCAATG	2 cut(s)	870, 1774
BseMII	CTCAG	5 cut(s)	206, 315, 329, 1116, 2529
BseRI	GAGGAG	4 cut(s)	473, 683, 1434, 2152
BseSI	GKGCMC	1 cut(s)	298
BseXI	GCAGC	6 cut(s)	829, 949, 952, 1366, 2344, 2971
BseYI	CCCAGC	2 cut(s)	626, 2516
BsgI	GTGCAG	3 cut(s)	816, 1177, 2343
BshFI	GGCC	4 cut(s)	1464, 2075, 2205, 2884
BshNI	GGYRCC	2 cut(s)	295, 1813
BsiHKAI	GWGCWC	5 cut(s)	44, 1285, 1380, 1398, 1993
BsiSI	CCGG	3 cut(s)	1471, 2040, 2891
BslI	CCNNNNNNNGG	4 cut(s)	237, 267, 2814, 2815
BsmAI	GTCTC	2 cut(s)	1006, 1130
BsmI	GAATGC	1 cut(s)	1638
BsnI	GGCC	4 cut(s)	1464, 2075, 2205, 2884
Bsp119I	TTCGAA	3 cut(s)	1541, 2456, 3013
Bsp1286I	GDGCHC	7 cut(s)	44, 298, 1285, 1380, 1398, 1993, 2506
Bsp13I	TCCGGA	1 cut(s)	2890
Bsp1407I	TGTACA	1 cut(s)	2493
Bsp143I	GATC	8 cut(s)	1288, 1791, 1828, 2283, 2349, 2567, 2578, 2733
Bsp1720I	GCTNAGC	1 cut(s)	10
BspACI	CCGC	4 cut(s)	434, 463, 2617, 2936
BspANI	GGCC	4 cut(s)	1464, 2075, 2205, 2884
BspCNI	CTCAG	5 cut(s)	205, 316, 330, 1117, 2530
BspEI	TCCGGA	1 cut(s)	2890
BspHI	TCATGA	2 cut(s)	409, 1747
BspLI	GGNNCC	4 cut(s)	297, 1815, 1830, 2120
BspMAI	CTGCAG	2 cut(s)	223, 2845
BspPI	GGATC	6 cut(s)	1799, 1823, 1836, 2344, 2562, 2586
BspQI	GCTCTTC	2 cut(s)	748, 2556
BspT104I	TTCGAA	3 cut(s)	1541, 2456, 3013
BspT107I	GGYRCC	2 cut(s)	295, 1813
BspTI	CTTAAG	1 cut(s)	1258
BsrBI	CCGCTC	1 cut(s)	463
BsrDI	GCAATG	2 cut(s)	870, 1774
BsrGI	TGTACA	1 cut(s)	2493
BssECI	CCNNGG	1 cut(s)	252
BssMI	GATC	8 cut(s)	1288, 1791, 1828, 2283, 2349, 2567, 2578, 2733
BssNAI	GTATAC	1 cut(s)	2586
BssSI	CACGAG	1 cut(s)	685
BssT1I	CCWWGG	1 cut(s)	252
Bst1107I	GTATAC	1 cut(s)	2586
Bst2BI	CACGAG	1 cut(s)	685
Bst2UI	CCWGG	2 cut(s)	1956, 2342
Bst6I	CTCTTC	6 cut(s)	512, 748, 1367, 1900, 1973, 2556
BstAFI	CTTAAG	1 cut(s)	1258
BstAPI	GCANNNNNTGC	1 cut(s)	839
BstAUI	TGTACA	1 cut(s)	2493
BstBI	TTCGAA	3 cut(s)	1541, 2456, 3013
BstC8I	GCNNGC	6 cut(s)	160, 1462, 1623, 2667, 2671, 3061
BstF5I	GGATG	6 cut(s)	334, 637, 654, 2554, 2699, 2783
BstHHI	GCGC	1 cut(s)	1638
BstKTI	GATC	8 cut(s)	1291, 1794, 1831, 2286, 2352, 2570, 2581, 2736
BstMAI	GTCTC	2 cut(s)	1006, 1130
BstMBI	GATC	8 cut(s)	1288, 1791, 1828, 2283, 2349, 2567, 2578, 2733
BstMWI	GCNNNNNNNGC	5 cut(s)	839, 851, 860, 1448, 1457
BstNI	CCWGG	2 cut(s)	1956, 2342
BstNSI	RCATGY	1 cut(s)	1022
BstPAI	GACNNNNGTC	1 cut(s)	900
BstSCI	CCNGG	2 cut(s)	1954, 2340
BstSFI	CTRYAG	2 cut(s)	219, 2841
BstSLI	GKGCMC	1 cut(s)	298
BstV1I	GCAGC	6 cut(s)	829, 949, 952, 1366, 2344, 2971
BstX2I	RGATCY	1 cut(s)	1828
BstXI	CCANNNNNNTGG	1 cut(s)	731
BstYI	RGATCY	1 cut(s)	1828
BstZ17I	GTATAC	1 cut(s)	2586
BsuI	GTATCC	1 cut(s)	250
BsuRI	GGCC	4 cut(s)	1464, 2075, 2205, 2884
BtrI	CACGTC	1 cut(s)	2093
BtsCI	GGATG	6 cut(s)	334, 637, 654, 2554, 2699, 2783
BtsI	GCAGTG	3 cut(s)	216, 813, 1449
BtsIMutI	CAGTG	9 cut(s)	187, 216, 382, 813, 1449, 1806, 2989, 3004, 3063
Cac8I	GCNNGC	6 cut(s)	160, 1462, 1623, 2667, 2671, 3061
CciI	TCATGA	2 cut(s)	409, 1747
CfoI	GCGC	1 cut(s)	1638
Cfr13I	GGNCC	1 cut(s)	2882
Csp6I	GTAC	8 cut(s)	887, 1037, 1255, 1814, 1909, 2268, 2494, 2821
CviQI	GTAC	8 cut(s)	887, 1037, 1255, 1814, 1909, 2268, 2494, 2821
DpnI	GATC	8 cut(s)	1290, 1793, 1830, 2285, 2351, 2569, 2580, 2735
DpnII	GATC	8 cut(s)	1288, 1791, 1828, 2283, 2349, 2567, 2578, 2733
DraI	TTTAAA	1 cut(s)	529
DraIII	CACNNNGTG	1 cut(s)	839
EaeI	YGGCCR	1 cut(s)	2073
Eam1104I	CTCTTC	6 cut(s)	512, 748, 1367, 1900, 1973, 2556
EarI	CTCTTC	6 cut(s)	512, 748, 1367, 1900, 1973, 2556
EciI	GGCGGA	1 cut(s)	449
Ecl136II	GAGCTC	2 cut(s)	42, 1396
Eco130I	CCWWGG	1 cut(s)	252
Eco147I	AGGCCT	1 cut(s)	2205
Eco24I	GRGCYC	3 cut(s)	44, 1398, 2506
Eco32I	GATATC	1 cut(s)	910
Eco53kI	GAGCTC	2 cut(s)	42, 1396
Eco57I	CTGAAG	3 cut(s)	405, 1061, 1509
EcoICRI	GAGCTC	2 cut(s)	42, 1396
EcoO109I	RGGNCCY	1 cut(s)	2882
EcoRII	CCWGG	2 cut(s)	1954, 2340
EcoRV	GATATC	1 cut(s)	910
EcoT14I	CCWWGG	1 cut(s)	252
EcoT22I	ATGCAT	2 cut(s)	1777, 2444
EcoT38I	GRGCYC	3 cut(s)	44, 1398, 2506
ErhI	CCWWGG	1 cut(s)	252
FalI	AAGNNNNNCTT	4 cut(s)	509, 541, 2625, 2657
FauI	CCCGC	1 cut(s)	2929
FauNDI	CATATG	4 cut(s)	1512, 1617, 1674, 2590
FbaI	TGATCA	1 cut(s)	2283
FblI	GTMKAC	1 cut(s)	2585
Fnu4HI	GCNGC	6 cut(s)	843, 963, 966, 1355, 2358, 2960
FokI	GGATG	6 cut(s)	341, 644, 661, 2561, 2686, 2790
FriOI	GRGCYC	3 cut(s)	44, 1398, 2506
Fsp4HI	GCNGC	6 cut(s)	843, 963, 966, 1355, 2358, 2960
FspAI	RTGCGCAY	1 cut(s)	1637
FspBI	CTAG	3 cut(s)	945, 1278, 2433
FspI	TGCGCA	1 cut(s)	1637
GlaI	GCGC	1 cut(s)	1637
GluI	GCNGC	6 cut(s)	843, 963, 966, 1355, 2358, 2960
GsaI	CCCAGC	2 cut(s)	630, 2520
GsuI	CTGGAG	2 cut(s)	1938, 2207
HaeIII	GGCC	4 cut(s)	1464, 2075, 2205, 2884
HapII	CCGG	3 cut(s)	1471, 2040, 2891
HhaI	GCGC	1 cut(s)	1638
Hin6I	GCGC	1 cut(s)	1636
HinP1I	GCGC	1 cut(s)	1636
HincII	GTYRAC	2 cut(s)	505, 1732
HindII	GTYRAC	2 cut(s)	505, 1732
HindIII	AAGCTT	3 cut(s)	1074, 2532, 2847
HpaII	CCGG	3 cut(s)	1471, 2040, 2891
HphI	GGTGA	2 cut(s)	287, 577
Hpy166II	GTNNAC	7 cut(s)	505, 1037, 1094, 1732, 2586, 2624, 2783
Hpy8I	GTNNAC	7 cut(s)	505, 1037, 1094, 1732, 2586, 2624, 2783
HpyCH4IV	ACGT	2 cut(s)	510, 2092
HpyF10VI	GCNNNNNNNGC	5 cut(s)	839, 851, 860, 1448, 1457
HpySE526I	ACGT	2 cut(s)	510, 2092
HspAI	GCGC	1 cut(s)	1636
Kpn2I	TCCGGA	1 cut(s)	2890
KpnI	GGTACC	1 cut(s)	1817
Ksp22I	TGATCA	1 cut(s)	2283
Kzo9I	GATC	8 cut(s)	1288, 1791, 1828, 2283, 2349, 2567, 2578, 2733
LguI	GCTCTTC	2 cut(s)	748, 2556
LmnI	GCTCC	4 cut(s)	47, 460, 1567, 2595
Lsp1109I	GCAGC	6 cut(s)	829, 949, 952, 1366, 2344, 2971
MaeI	CTAG	3 cut(s)	945, 1278, 2433
MaeII	ACGT	2 cut(s)	510, 2092
MaeIII	GTNAC	6 cut(s)	214, 415, 587, 927, 1444, 2794
MalI	GATC	8 cut(s)	1290, 1793, 1830, 2285, 2351, 2569, 2580, 2735
MbiI	CCGCTC	1 cut(s)	463
MboI	GATC	8 cut(s)	1288, 1791, 1828, 2283, 2349, 2567, 2578, 2733
MfeI	CAATTG	1 cut(s)	2248
MflI	RGATCY	1 cut(s)	1828
MhlI	GDGCHC	7 cut(s)	44, 298, 1285, 1380, 1398, 1993, 2506
MlsI	TGGCCA	1 cut(s)	2075
MluNI	TGGCCA	1 cut(s)	2075
MlyI	GAGTC	5 cut(s)	1008, 1802, 1953, 1990, 2663
MmeI	TCCRAC	4 cut(s)	468, 730, 1785, 1806
Mox20I	TGGCCA	1 cut(s)	2075
Mph1103I	ATGCAT	2 cut(s)	1777, 2444
MroI	TCCGGA	1 cut(s)	2890
MroXI	GAANNNNTTC	1 cut(s)	1046
MscI	TGGCCA	1 cut(s)	2075
MseI	TTAA	9 cut(s)	315, 528, 702, 1259, 1530, 1845, 2331, 2705, 3033
MslI	CAYNNNNRTG	2 cut(s)	2828, 3068
Msp20I	TGGCCA	1 cut(s)	2075
MspCI	CTTAAG	1 cut(s)	1258
MspI	CCGG	3 cut(s)	1471, 2040, 2891
MspR9I	CCNGG	2 cut(s)	1956, 2342
MunI	CAATTG	1 cut(s)	2248
Mva1269I	GAATGC	1 cut(s)	1638
MvaI	CCWGG	2 cut(s)	1956, 2342
MwoI	GCNNNNNNNGC	5 cut(s)	839, 851, 860, 1448, 1457
NdeI	CATATG	4 cut(s)	1512, 1617, 1674, 2590
NdeII	GATC	8 cut(s)	1288, 1791, 1828, 2283, 2349, 2567, 2578, 2733
NlaIV	GGNNCC	4 cut(s)	297, 1815, 1830, 2120
NmeAIII	GCCGAG	1 cut(s)	333
NmuCI	GTSAC	4 cut(s)	214, 587, 1444, 2794
NsbI	TGCGCA	1 cut(s)	1637
NsiI	ATGCAT	2 cut(s)	1777, 2444
NspI	RCATGY	1 cut(s)	1022
NspV	TTCGAA	3 cut(s)	1541, 2456, 3013
PagI	TCATGA	2 cut(s)	409, 1747
PceI	AGGCCT	1 cut(s)	2205
PciI	ACATGT	1 cut(s)	1018
PciSI	GCTCTTC	2 cut(s)	748, 2556
PctI	GAATGC	1 cut(s)	1638
PdmI	GAANNNNTTC	1 cut(s)	1046
PfeI	GAWTC	8 cut(s)	491, 1009, 1065, 1310, 1597, 1885, 2148, 2447
PfoI	TCCNGGA	2 cut(s)	1954, 2340
PkrI	GCNGC	6 cut(s)	844, 964, 967, 1356, 2359, 2961
PleI	GAGTC	5 cut(s)	1007, 1802, 1953, 1989, 2662
PpsI	GAGTC	5 cut(s)	1007, 1802, 1953, 1989, 2662
PscI	ACATGT	1 cut(s)	1018
PshAI	GACNNNNGTC	1 cut(s)	900
PsiI	TTATAA	1 cut(s)	2966
Psp124BI	GAGCTC	2 cut(s)	44, 1398
Psp1406I	AACGTT	1 cut(s)	510
Psp6I	CCWGG	2 cut(s)	1954, 2340
PspFI	CCCAGC	2 cut(s)	626, 2516
PspGI	CCWGG	2 cut(s)	1954, 2340
PspN4I	GGNNCC	4 cut(s)	297, 1815, 1830, 2120
PspPI	GGNCC	1 cut(s)	2882
PstI	CTGCAG	2 cut(s)	223, 2845
PsuI	RGATCY	1 cut(s)	1828
RsaI	GTAC	8 cut(s)	888, 1038, 1256, 1815, 1910, 2269, 2495, 2822
RsaNI	GTAC	8 cut(s)	887, 1037, 1255, 1814, 1909, 2268, 2494, 2821
RseI	CAYNNNNRTG	2 cut(s)	2828, 3068
SacI	GAGCTC	2 cut(s)	44, 1398
SapI	GCTCTTC	2 cut(s)	748, 2556
SaqAI	TTAA	9 cut(s)	315, 528, 702, 1259, 1530, 1845, 2331, 2705, 3033
SatI	GCNGC	6 cut(s)	843, 963, 966, 1355, 2358, 2960
Sau3AI	GATC	8 cut(s)	1288, 1791, 1828, 2283, 2349, 2567, 2578, 2733
Sau96I	GGNCC	1 cut(s)	2882
SchI	GAGTC	5 cut(s)	1008, 1802, 1953, 1990, 2663
ScrFI	CCNGG	2 cut(s)	1956, 2342
SduI	GDGCHC	7 cut(s)	44, 298, 1285, 1380, 1398, 1993, 2506
SfcI	CTRYAG	2 cut(s)	219, 2841
SfuI	TTCGAA	3 cut(s)	1541, 2456, 3013
SmiMI	CAYNNNNRTG	2 cut(s)	2828, 3068
SmlI	CTYRAG	3 cut(s)	1097, 1258, 1601
SmoI	CTYRAG	3 cut(s)	1097, 1258, 1601
SseBI	AGGCCT	1 cut(s)	2205
SsiI	CCGC	4 cut(s)	434, 463, 2617, 2936
SspMI	CTAG	3 cut(s)	945, 1278, 2433
SstI	GAGCTC	2 cut(s)	44, 1398
StuI	AGGCCT	1 cut(s)	2205
StyD4I	CCNGG	2 cut(s)	1954, 2340
StyI	CCWWGG	1 cut(s)	252
TaiI	ACGT	2 cut(s)	513, 2095
TatI	WGTACW	3 cut(s)	1036, 2267, 2493
TfiI	GAWTC	8 cut(s)	491, 1009, 1065, 1310, 1597, 1885, 2148, 2447
Tru1I	TTAA	9 cut(s)	315, 528, 702, 1259, 1530, 1845, 2331, 2705, 3033
Tru9I	TTAA	9 cut(s)	315, 528, 702, 1259, 1530, 1845, 2331, 2705, 3033
TscAI	CASTG	9 cut(s)	187, 223, 382, 813, 1449, 1806, 2989, 3004, 3070
TseFI	GTSAC	4 cut(s)	214, 587, 1444, 2794
TseI	GCWGC	6 cut(s)	842, 962, 965, 1354, 2357, 2959
Tsp45I	GTSAC	4 cut(s)	214, 587, 1444, 2794
TspGWI	ACGGA	3 cut(s)	1358, 2342, 3042
TspRI	CASTG	9 cut(s)	187, 223, 382, 813, 1449, 1806, 2989, 3004, 3070
Vha464I	CTTAAG	1 cut(s)	1258
XapI	RAATTY	6 cut(s)	785, 869, 1578, 1736, 1875, 2751
XbaI	TCTAGA	1 cut(s)	1277
XceI	RCATGY	1 cut(s)	1022
XmiI	GTMKAC	1 cut(s)	2585
XmnI	GAANNNNTTC	1 cut(s)	1046
XspI	CTAG	3 cut(s)	945, 1278, 2433
Zsp2I	ATGCAT	2 cut(s)	1777, 2444

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.