Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007077

Physical Location & Seq

Genomic Coordinates Reverse (-)

4951235 .. 4954522

Gene Structure 3288 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1044100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3150 bp

ATGAGAAGCTTAATCAAACTACAACGCTTGTGTTCGAGCTCAACCTGCAAGATTATCCATTTCCGCTTCTCAAACGTGTACAAGATTGATCGTTTTGCAACTGTTGCTCAGCCATCAAAGCCTAATAAGAAAACCCACTCCAAACCAGCACCAGAAAACGGCGGATTTGGCTCGCTCTTTGCTGAAATTACTGAGATTTTGGGAGCTGAAAACGTTACTGCGGATAAAACCCCATCTGGGTTTTTTATTTCGGAGGAAACCCCGGGGAGGGTTGGTGAGGCTGGGGAAGATTGCCAACCTTGCACGCAAACTGTTTGTAAAAATGCCGAGGAGAGTGTACTGCAAGAAAAGAAAGATATAACAGGTTTGGAGGATGCACAGGTGGAAAATTTGGCTGAAAGTGATGTTAGCCCCGTGGTTCAGGAGGTTATGAAGATTGTCAGGGCGGAGAGTGGTTTGGTTTCAATGGAGGAGATGTTAGAAAAATTGGGTTTTCCGTTGGATTCAGATATTGTTGACAAAGTGTTGAAGAGGTGCTTTAAAGTGCCACATTTGGCTTGGAGGTTCTTCAATTGGGTGAAGCTCAAAGAAGGGTTTTGTCATACAACTAAGACCTATAATACCATGCTGTTTATAGCTGGGGATGCAAAAGAGTTCGGGATGGTGGAGAAGTTGATGGAGGAAATGGAAAAACATCTGTGCGGGAAAGATGTTAAGACTTGGACTATTCTCATCTCACAGTATGGAAATGCTAAGTTTATGGGCAAAGCATTGTTCTTCTACGAAAAGATGAGGAAAGAAGGTTTTGAACCGGATGTAGTGGTTTACAGGTCGATGATACGTGCACTGTGTAATACTGGAAAACCTGAAGTTGCGATGGAATTTTACAAGGAGATGGTACAGAAGGACATGGGACTTGACATAAATTTATACAAGTTGTTATTAAATGGTATAGCGAGCTCCGGCGATACTGCTTCTATTGCCTTGGTTTCAGATGACATGATTAGAGTTTCACAGATTCCAGAACACATTGTTTATGGTTGTGTGTTGAAGAGTTTCTGCATATCTGGAAGAATCAGAGAAGCTTTGGAATTTATTCGTGAACTCAAGAATAAAGAGGTTATGCTTGGCCCCGAGTATTTTGAGACTTTGGTGAAAGGACTGTGCATGGCTGACAGAATTACAGACGCTTTGGAAATTTTGGAAATCATGAAGAGGAGAAATATTGTTGATGGGAAGGTTTATGGGATCATCATCAATGGGTACTTAAGAAATAATGATGCTGATAAAGCGCTAGATCTGTTTCATAGCATGAAAGAATCTGGATACGTGCCCACAACTTCTACCTACACAGAACTGATGCAACACCTCTTCAAGTTGAATGAATTCCAAAGAGGCGGTGATCTGTATAATGAGATGCTGGAAAGAGGAGTTGAGCCAGATATTGTGGCGATCACAGCCATGGTTGCAGGTCAGGTTCGTCAAAACCATATATCTGAAGCATGGAAAATATTTAATAGTATGAAGGACAAAGGCATCAAGCCCACATTGAAGTCGTATTCTATATTCATTAAGGAGCTTTGTAGAATTTCAAGGACAGATGAGATTCTCAAGGTCTTTGATGACATGCGAGCATCTGATATAGTAATTCAAGATGACATATTTAATATGGCTATGTATCATATGGAGAAAAAGGGAGAGATTGATAACCTTGAAAAAGTAAAGCAGTTGCAGAGGATCTATAAACTTCATCCCCGAGAAAGAGAAGAGGTATCTAATAATGAAGCATCTAGGGGTGAGGAGCTCAGTACAGGGACGGACTTTAATTACTTACAACCAGCAAAGATGGATTGTACATTAGTGGAGCCACTTTTAAAGGATTATGATGAGCATCGTTTGCAAGAAATTTGTAAAATTCTGTCCTCTTCAACAGATTGGCCCTTAGTTCAGGAAGCTTTGAAGAATTCTGCTGTTGATTTTACTCCAGGACTCGTTGTGGAAATTTTGAGGAATAGCAGCATGCATGGTTTCGTAGCATTACATTTTTTTGCATGGGTAGGAAAGCAAACTGGTTATAGCCACACTACTGACACTTACAACATGGCTATCAAAACCGCAGGGCGCGGGAAAGATTTCAAGCACATGAGAAACCTCTTTTATGAAATGAGAAGAAAAGGTTTCTTAATCACACCAGATACATGGACAATAATGATAATGCAATATGGTCGAACAGGTATGACAGAGATTGCTCTGCGGATTTTTGGTGAGATGAAGTCTAGTAATTGCCACCCAACTCTCAGTACCTACAAGTATTTAGTCATATCTCTTTGTGGGAGAAAAGGTAGAAAGGTAGACGAAGCAATTAGAATTTTCAAGGAGATGATCCGTGCCAGTCATGTACCTGACAAAGAGTTGGTTGAAACGTATATTGGTTGTTTATGTGAAGTTGGTAAGCTCTCAGATGCCAGAAGGTGCATAGATTCTCTTCCTAAAGTTGGTTTTTCGATTCCTCTTAGTTATTCCTTGTACATTAGGGCTCTATGTCGAGCCGGGAGGTTACAAGAAGCTTCAGCATTGATGGATGATGTTGGGGAAGACCGGTCAAAACTGGATCAGTATACTTATGGGAGCCTTGTACATGGACTTCTGCGGAGTGGACAATTAGAAGCAGCACTGGCCAAGGTGGATTCAATGAAGCAGGCGGGTGTAAATCCAACCGTCCATGTTTATACATCCTTAATAGTTCACTACTTGAGAGAGAAGCAGATAGGAAAGGCTTTAGAAATTTTTAAGGAAATGCAACAGAAGGGTTGTAGACCAACAGTCATTACCTATTCTGCACTTATTCGAGGGTACATGAACATGGAAATGGTTTCCGAAGCTTGGGATGTCTTTCACAACATGAAACTTAAAGGAACATTGCCAGATTTTAGAACGTATTCGATGTTCATCACTTGTCTTTGTAAGGTGGGCAAATCTGAAGAAGCAATGCAGCTTATAACTGAAATGTTGGAAAGTGGGATTGTTCCGAGTGTGGTGAATTTTCGAACCATATTTTACGGGCTTAACAGGGAAGGTAAGCAAGATTTGGCTCGCACTGTAATGGAGCAAAAGTTATCCTTAATAAGAAGCCGCAAGGTGCTAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1050

Amino Acids

119.41

Weight (kDa)

7.68

Isoelectric Point (pI)

27.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	236 - 285	4.9e-11	PPR repeat family
PPR_1	PF12854	268 - 300	1.3e-06	PPR repeat
PPR_2	PF13041	271 - 319	6.9e-08	PPR repeat family
PPR	PF01535	274 - 303	5.1e-06	PPR repeat
PPR_2	PF13041	414 - 458	3.7e-10	PPR repeat family
PPR	PF01535	414 - 442	3.7e-06	PPR repeat
PPR_long	PF17177	423 - 553	1.4e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	445 - 490	6e-08	PPR repeat family
PPR_3	PF13812	469 - 525	2.3e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	480 - 529	5.7e-14	PPR repeat family
PPR_3	PF13812	718 - 778	8.1e-07	Pentatricopeptide repeat domain
PPR	PF01535	733 - 762	2.3e-06	PPR repeat
PPR_2	PF13041	733 - 777	2.7e-08	PPR repeat family
TPR_24	PF23276	853 - 967	7e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	859 - 915	2.4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	871 - 914	9.6e-07	PPR repeat family
PPR_long	PF17177	884 - 1019	1.7e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	905 - 953	1.2e-14	PPR repeat family
TPR_24	PF23276	908 - 999	3.4e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	909 - 952	7.2e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	936 - 969	5.5e-06	PPR repeat
PPR_2	PF13041	940 - 989	3.5e-14	PPR repeat family
PPR_1	PF12854	972 - 1003	4e-07	PPR repeat
PPR_2	PF13041	979 - 1022	1.6e-07	PPR repeat family
PPR	PF01535	979 - 1008	1e-05	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06400
fragaria_vesca	FvH4_6g20810 FvH4_6g20810 FvH4_6g20810
malus_domestica	MD12G1044100.v1.1
prunus_persica	Prupe.7G091000_v2.0.a1
pyrus_communis	pycom12g03800
rosa_chinensis	RchiOBHm_Chr3g0476881
rosa_laevigata	RLG00000023746
rosa_multiflora	Rmu_sc0000288.1_g000033
rosa_roxburghii	Rroxscaffold_6G00403680 Rroxscaffold_6G00404930
rosa_rugosa	Rorug03G0157400
rosa_samantha	Rh3AG207600 Rh3AG229900 Rh3BG240200 Rh3BG241300 Rh3CG233900 Rh3CG235000 Rh3CG235700 Rh3CG259900 Rh3DG233900
rosa_wichuraiana	Rw0G011140 Rw3G018900 Rw3G020670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2999
Acc36I	ACCTGC	2 cut(s)	53, 1460
AccI	GTMKAC	3 cut(s)	2352, 2618, 2815
AccII	CGCG	1 cut(s)	2124
AclI	AACGTT	1 cut(s)	213
AclWI	GGATC	4 cut(s)	1256, 1745, 2377, 2619
AcoI	YGGCCR	1 cut(s)	2676
AcuI	CTGAAG	4 cut(s)	888, 1518, 2553, 3000
AfeI	AGCGCT	1 cut(s)	1293
AfiI	CCNNNNNNNGG	6 cut(s)	158, 237, 267, 268, 2495, 2883
AflII	CTTAAG	1 cut(s)	1267
AflIII	ACRYGT	1 cut(s)	75
AgeI	ACCGGT	1 cut(s)	2598
AhdI	GACNNNNNGTC	1 cut(s)	2822
AjnI	CCWGG	1 cut(s)	1984
AjuI	GAANNNNNNNTTGG	2 cut(s)	2412, 2444
Alw21I	GWGCWC	4 cut(s)	41, 847, 962, 1806
Alw26I	GTCTC	1 cut(s)	1139
Alw44I	GTGCAC	1 cut(s)	843
AlwI	GGATC	4 cut(s)	1256, 1745, 2377, 2619
Ama87I	CYCGRG	3 cut(s)	262, 1133, 1755
Aor51HI	AGCGCT	1 cut(s)	1293
AoxI	GGCC	3 cut(s)	1129, 1937, 2676
ApaLI	GTGCAC	1 cut(s)	843
ApeKI	GCWGC	3 cut(s)	2016, 2669, 2992
AsiGI	ACCGGT	1 cut(s)	2598
Asp700I	GAANNNNTTC	5 cut(s)	1055, 1095, 1385, 2132, 2938
AspLEI	GCGC	2 cut(s)	1294, 2124
AspS9I	GGNCC	2 cut(s)	1130, 1938
AsuC2I	CCSGG	3 cut(s)	263, 264, 2551
AsuHPI	GGTGA	7 cut(s)	287, 589, 1165, 1412, 1808, 2276, 3049
AsuII	TTCGAA	1 cut(s)	3046
AvaI	CYCGRG	3 cut(s)	262, 1133, 1755
BaeGI	GKGCMC	2 cut(s)	847, 1335
BalI	TGGCCA	1 cut(s)	2678
BanII	GRGCYC	4 cut(s)	41, 962, 1806, 2539
BarI	GAAGNNNNNNTAC	2 cut(s)	2628, 2660
BbsI	GAAGAC	1 cut(s)	2601
Bbv12I	GWGCWC	4 cut(s)	41, 847, 962, 1806
BbvI	GCAGC	3 cut(s)	2028, 2681, 3004
BccI	CCATC	9 cut(s)	121, 241, 655, 670, 871, 889, 1226, 1840, 2572
BceAI	ACGGC	1 cut(s)	175
BcgI	CGANNNNNNTGC	2 cut(s)	2207, 2241
BciT130I	CCWGG	1 cut(s)	1986
BciVI	GTATCC	1 cut(s)	1319
BcnI	CCSGG	3 cut(s)	263, 264, 2551
BcoDI	GTCTC	1 cut(s)	1139
BfaI	CTAG	3 cut(s)	1295, 1791, 2277
BfoI	RGCGCY	1 cut(s)	1295
BfrI	CTTAAG	1 cut(s)	1267
BfuAI	ACCTGC	2 cut(s)	53, 1460
BfuI	GTATCC	1 cut(s)	1319
BglII	AGATCT	1 cut(s)	1297
BisI	GCNGC	4 cut(s)	2017, 2670, 2993, 3133
BlpI	GCTNAGC	1 cut(s)	108
BlsI	GCNGC	4 cut(s)	2018, 2671, 2994, 3134
Bme1390I	CCNGG	4 cut(s)	263, 264, 1986, 2551
BmeRI	GACNNNNNGTC	1 cut(s)	2822
BmeT110I	CYCGRG	3 cut(s)	262, 1133, 1755
BmgT120I	GGNCC	2 cut(s)	1130, 1938
BmiI	GGNNCC	3 cut(s)	1132, 1866, 2630
BmrFI	CCNGG	4 cut(s)	263, 264, 1986, 2551
BpiI	GAAGAC	1 cut(s)	2601
BpmI	CTGGAG	1 cut(s)	1968
Bpu1102I	GCTNAGC	1 cut(s)	108
Bpu14I	TTCGAA	1 cut(s)	3046
BpuEI	CTTGAG	3 cut(s)	1091, 1595, 2773
BpuMI	CCSGG	3 cut(s)	263, 264, 2551
BsaAI	YACGTR	2 cut(s)	842, 1330
BsaBI	GATNNNNATC	3 cut(s)	1253, 1890, 2948
BsaJI	CCNNGG	8 cut(s)	261, 262, 263, 327, 414, 984, 1461, 2679
BsaWI	WCCGGW	2 cut(s)	811, 2598
BsaXI	ACNNNNNCTCC	8 cut(s)	259, 289, 461, 491, 2326, 2356, 3098, 3128
Bsc4I	CCNNNNNNNGG	6 cut(s)	158, 237, 267, 268, 2495, 2883
Bse118I	RCCGGY	1 cut(s)	2598
Bse1I	ACTGG	5 cut(s)	862, 2074, 2391, 2613, 2679
Bse3DI	GCAATG	2 cut(s)	2918, 2994
Bse8I	GATNNNNATC	3 cut(s)	1253, 1890, 2948
BseBI	CCWGG	1 cut(s)	1986
BseDI	CCNNGG	8 cut(s)	261, 262, 263, 327, 414, 984, 1461, 2679
BseGI	GGATG	8 cut(s)	379, 649, 666, 820, 1750, 2587, 2732, 2893
BseJI	GATNNNNATC	3 cut(s)	1253, 1890, 2948
BseLI	CCNNNNNNNGG	6 cut(s)	158, 237, 267, 268, 2495, 2883
BseMI	GCAATG	2 cut(s)	2918, 2994
BseMII	CTCAG	5 cut(s)	122, 183, 1819, 2311, 2472
BseNI	ACTGG	5 cut(s)	862, 2074, 2391, 2613, 2679
BseRI	GAGGAG	5 cut(s)	344, 485, 1231, 1443, 1814
BseSI	GKGCMC	2 cut(s)	847, 1335
BseXI	GCAGC	3 cut(s)	2028, 2681, 3004
BseYI	CCCAGC	2 cut(s)	281, 638
BsgI	GTGCAG	1 cut(s)	2823
Bsh1236I	CGCG	1 cut(s)	2124
BshFI	GGCC	3 cut(s)	1131, 1939, 2678
BshTI	ACCGGT	1 cut(s)	2598
BsiHKAI	GWGCWC	4 cut(s)	41, 847, 962, 1806
BsiHKCI	CYCGRG	3 cut(s)	262, 1133, 1755
BsiSI	CCGG	5 cut(s)	263, 812, 963, 2550, 2599
BslFI	GGGAC	2 cut(s)	927, 1828
BslI	CCNNNNNNNGG	6 cut(s)	158, 237, 267, 268, 2495, 2883
BsmAI	GTCTC	1 cut(s)	1139
BsmFI	GGGAC	2 cut(s)	927, 1828
BsnI	GGCC	3 cut(s)	1131, 1939, 2678
BsoBI	CYCGRG	3 cut(s)	262, 1133, 1755
Bsp119I	TTCGAA	1 cut(s)	3046
Bsp1286I	GDGCHC	6 cut(s)	41, 847, 962, 1335, 1806, 2539
Bsp1407I	TGTACA	4 cut(s)	78, 1853, 2526, 2635
Bsp143I	GATC	8 cut(s)	88, 1248, 1297, 1402, 1452, 1737, 2382, 2611
Bsp1720I	GCTNAGC	1 cut(s)	108
Bsp19I	CCATGG	1 cut(s)	1461
BspANI	GGCC	3 cut(s)	1131, 1939, 2678
BspCNI	CTCAG	5 cut(s)	121, 184, 1818, 2310, 2471
BspFNI	CGCG	1 cut(s)	2124
BspHI	TCATGA	1 cut(s)	1209
BspLI	GGNNCC	3 cut(s)	1132, 1866, 2630
BspMI	ACCTGC	2 cut(s)	53, 1460
BspPI	GGATC	4 cut(s)	1256, 1745, 2377, 2619
BspT104I	TTCGAA	1 cut(s)	3046
BspTI	CTTAAG	1 cut(s)	1267
BsrDI	GCAATG	2 cut(s)	2918, 2994
BsrFI	RCCGGY	1 cut(s)	2598
BsrGI	TGTACA	4 cut(s)	78, 1853, 2526, 2635
BsrI	ACTGG	5 cut(s)	862, 2074, 2391, 2613, 2679
BssAI	RCCGGY	1 cut(s)	2598
BssECI	CCNNGG	8 cut(s)	261, 262, 263, 327, 414, 984, 1461, 2679
BssMI	GATC	8 cut(s)	88, 1248, 1297, 1402, 1452, 1737, 2382, 2611
BssNAI	GTATAC	1 cut(s)	2619
BssT1I	CCWWGG	3 cut(s)	984, 1461, 2679
Bst1107I	GTATAC	1 cut(s)	2619
Bst2UI	CCWGG	1 cut(s)	1986
Bst4CI	ACNGT	8 cut(s)	103, 313, 741, 849, 1164, 2719, 2824, 3100
Bst6I	CTCTTC	7 cut(s)	524, 1046, 1208, 1376, 1761, 1930, 2490
BstAFI	CTTAAG	1 cut(s)	1267
BstAPI	GCANNNNNTGC	2 cut(s)	104, 1897
BstAUI	TGTACA	4 cut(s)	78, 1853, 2526, 2635
BstBAI	YACGTR	2 cut(s)	842, 1330
BstBI	TTCGAA	1 cut(s)	3046
BstC8I	GCNNGC	7 cut(s)	173, 305, 958, 1632, 2021, 2700, 3094
BstDEI	CTNAG	9 cut(s)	108, 192, 609, 753, 1805, 1942, 2297, 2458, 2513
BstDSI	CCRYGG	2 cut(s)	414, 1461
BstF5I	GGATG	8 cut(s)	379, 649, 666, 820, 1750, 2587, 2732, 2893
BstFNI	CGCG	1 cut(s)	2124
BstH2I	RGCGCY	1 cut(s)	1295
BstHHI	GCGC	2 cut(s)	1294, 2124
BstKTI	GATC	8 cut(s)	91, 1251, 1300, 1405, 1455, 1740, 2385, 2614
BstMAI	GTCTC	1 cut(s)	1139
BstMBI	GATC	8 cut(s)	88, 1248, 1297, 1402, 1452, 1737, 2382, 2611
BstNI	CCWGG	1 cut(s)	1986
BstNSI	RCATGY	2 cut(s)	1630, 2023
BstSCI	CCNGG	4 cut(s)	261, 262, 1984, 2549
BstSLI	GKGCMC	2 cut(s)	847, 1335
BstUI	CGCG	1 cut(s)	2124
BstV1I	GCAGC	3 cut(s)	2028, 2681, 3004
BstV2I	GAAGAC	1 cut(s)	2601
BstX2I	RGATCY	2 cut(s)	1297, 1737
BstXI	CCANNNNNNTGG	1 cut(s)	2199
BstYI	RGATCY	2 cut(s)	1297, 1737
BstZ17I	GTATAC	1 cut(s)	2619
BsuI	GTATCC	1 cut(s)	1319
BsuRI	GGCC	3 cut(s)	1131, 1939, 2678
BtgI	CCRYGG	2 cut(s)	414, 1461
BtgZI	GCGATG	1 cut(s)	890
BtsCI	GGATG	8 cut(s)	379, 649, 666, 820, 1750, 2587, 2732, 2893
BtsIMutI	CAGTG	3 cut(s)	845, 2672, 3096
BveI	ACCTGC	2 cut(s)	53, 1460
Cac8I	GCNNGC	7 cut(s)	173, 305, 958, 1632, 2021, 2700, 3094
CciI	TCATGA	1 cut(s)	1209
CfoI	GCGC	2 cut(s)	1294, 2124
Cfr10I	RCCGGY	1 cut(s)	2598
Cfr13I	GGNCC	2 cut(s)	1130, 1938
Cfr9I	CCCGGG	1 cut(s)	262
CseI	GACGC	1 cut(s)	1196
CspAI	ACCGGT	1 cut(s)	2598
DdeI	CTNAG	9 cut(s)	108, 192, 609, 753, 1805, 1942, 2297, 2458, 2513
DpnI	GATC	8 cut(s)	90, 1250, 1299, 1404, 1454, 1739, 2384, 2613
DpnII	GATC	8 cut(s)	88, 1248, 1297, 1402, 1452, 1737, 2382, 2611
DraI	TTTAAA	2 cut(s)	541, 1875
DriI	GACNNNNNGTC	1 cut(s)	2822
EaeI	YGGCCR	1 cut(s)	2676
Eam1104I	CTCTTC	7 cut(s)	524, 1046, 1208, 1376, 1761, 1930, 2490
Eam1105I	GACNNNNNGTC	1 cut(s)	2822
EarI	CTCTTC	7 cut(s)	524, 1046, 1208, 1376, 1761, 1930, 2490
EciI	GGCGGA	2 cut(s)	177, 461
Ecl136II	GAGCTC	3 cut(s)	39, 960, 1804
Eco130I	CCWWGG	3 cut(s)	984, 1461, 2679
Eco24I	GRGCYC	4 cut(s)	41, 962, 1806, 2539
Eco47III	AGCGCT	1 cut(s)	1293
Eco53kI	GAGCTC	3 cut(s)	39, 960, 1804
Eco57I	CTGAAG	4 cut(s)	888, 1518, 2553, 3000
Eco88I	CYCGRG	3 cut(s)	262, 1133, 1755
EcoICRI	GAGCTC	3 cut(s)	39, 960, 1804
EcoRI	GAATTC	2 cut(s)	1385, 1963
EcoRII	CCWGG	1 cut(s)	1984
EcoT14I	CCWWGG	3 cut(s)	984, 1461, 2679
EcoT22I	ATGCAT	1 cut(s)	2025
EcoT38I	GRGCYC	4 cut(s)	41, 962, 1806, 2539
ErhI	CCWWGG	3 cut(s)	984, 1461, 2679
FalI	AAGNNNNNCTT	4 cut(s)	521, 553, 3104, 3136
FaqI	GGGAC	2 cut(s)	927, 1828
FauI	CCCGC	3 cut(s)	695, 2117, 2695
FauNDI	CATATG	1 cut(s)	1683
FblI	GTMKAC	3 cut(s)	2352, 2618, 2815
Fnu4HI	GCNGC	4 cut(s)	2017, 2670, 2993, 3133
FokI	GGATG	8 cut(s)	386, 656, 673, 827, 1737, 2594, 2719, 2900
FriOI	GRGCYC	4 cut(s)	41, 962, 1806, 2539
Fsp4HI	GCNGC	4 cut(s)	2017, 2670, 2993, 3133
FspBI	CTAG	3 cut(s)	1295, 1791, 2277
GlaI	GCGC	2 cut(s)	1293, 2123
GluI	GCNGC	4 cut(s)	2017, 2670, 2993, 3133
GsaI	CCCAGC	2 cut(s)	285, 642
GsuI	CTGGAG	1 cut(s)	1968
HaeII	RGCGCY	1 cut(s)	1295
HaeIII	GGCC	3 cut(s)	1131, 1939, 2678
HapII	CCGG	5 cut(s)	263, 812, 963, 2550, 2599
HgaI	GACGC	1 cut(s)	1196
HhaI	GCGC	2 cut(s)	1294, 2124
Hin6I	GCGC	2 cut(s)	1292, 2122
HinP1I	GCGC	2 cut(s)	1292, 2122
HincII	GTYRAC	1 cut(s)	517
HindII	GTYRAC	1 cut(s)	517
HindIII	AAGCTT	5 cut(s)	7, 1083, 1953, 2565, 2880
HinfI	GANTC	9 cut(s)	503, 1018, 1074, 1319, 1606, 1989, 2480, 2506, 2687
HpaII	CCGG	5 cut(s)	263, 812, 963, 2550, 2599
HphI	GGTGA	7 cut(s)	287, 589, 1165, 1412, 1808, 2276, 3049
HpyAV	CCTTC	8 cut(s)	584, 794, 898, 1231, 1519, 2463, 2800, 3068
HpyCH4III	ACNGT	8 cut(s)	103, 313, 741, 849, 1164, 2719, 2824, 3100
HpyCH4IV	ACGT	6 cut(s)	75, 213, 841, 1329, 2423, 2936
HpyF3I	CTNAG	9 cut(s)	108, 192, 609, 753, 1805, 1942, 2297, 2458, 2513
HpySE526I	ACGT	6 cut(s)	75, 213, 841, 1329, 2423, 2936
HspAI	GCGC	2 cut(s)	1292, 2122
Kzo9I	GATC	8 cut(s)	88, 1248, 1297, 1402, 1452, 1737, 2382, 2611
LmnI	GCTCC	7 cut(s)	203, 965, 1576, 1801, 1864, 2628, 3106
Lsp1109I	GCAGC	3 cut(s)	2028, 2681, 3004
MaeI	CTAG	3 cut(s)	1295, 1791, 2277
MaeII	ACGT	6 cut(s)	75, 213, 841, 1329, 2423, 2936
MaeIII	GTNAC	2 cut(s)	214, 2556
MalI	GATC	8 cut(s)	90, 1250, 1299, 1404, 1454, 1739, 2384, 2613
MboI	GATC	8 cut(s)	88, 1248, 1297, 1402, 1452, 1737, 2382, 2611
MfeI	CAATTG	1 cut(s)	571
MflI	RGATCY	2 cut(s)	1297, 1737
MhlI	GDGCHC	6 cut(s)	41, 847, 962, 1335, 1806, 2539
MlsI	TGGCCA	1 cut(s)	2678
MluNI	TGGCCA	1 cut(s)	2678
MlyI	GAGTC	1 cut(s)	1983
MmeI	TCCRAC	3 cut(s)	480, 2738, 2991
Mox20I	TGGCCA	1 cut(s)	2678
Mph1103I	ATGCAT	1 cut(s)	2025
MroXI	GAANNNNTTC	5 cut(s)	1055, 1095, 1385, 2132, 2938
MscI	TGGCCA	1 cut(s)	2678
MslI	CAYNNNNRTG	4 cut(s)	1460, 2861, 2867, 3101
Msp20I	TGGCCA	1 cut(s)	2678
MspCI	CTTAAG	1 cut(s)	1267
MspI	CCGG	5 cut(s)	263, 812, 963, 2550, 2599
MspR9I	CCNGG	4 cut(s)	263, 264, 1986, 2551
MunI	CAATTG	1 cut(s)	571
MvaI	CCWGG	1 cut(s)	1986
MvnI	CGCG	1 cut(s)	2124
NciI	CCSGG	3 cut(s)	263, 264, 2551
NcoI	CCATGG	1 cut(s)	1461
NdeI	CATATG	1 cut(s)	1683
NdeII	GATC	8 cut(s)	88, 1248, 1297, 1402, 1452, 1737, 2382, 2611
NlaIV	GGNNCC	3 cut(s)	1132, 1866, 2630
NmeAIII	GCCGAG	1 cut(s)	352
NsiI	ATGCAT	1 cut(s)	2025
NspI	RCATGY	2 cut(s)	1630, 2023
NspV	TTCGAA	1 cut(s)	3046
PaeI	GCATGC	1 cut(s)	2023
PagI	TCATGA	1 cut(s)	1209
PdmI	GAANNNNTTC	5 cut(s)	1055, 1095, 1385, 2132, 2938
PfeI	GAWTC	8 cut(s)	503, 1018, 1074, 1319, 1606, 2480, 2506, 2687
PfoI	TCCNGGA	1 cut(s)	1984
PinAI	ACCGGT	1 cut(s)	2598
PkrI	GCNGC	4 cut(s)	2018, 2671, 2994, 3134
PleI	GAGTC	1 cut(s)	1983
PpsI	GAGTC	1 cut(s)	1983
Ppu21I	YACGTR	2 cut(s)	842, 1330
PsiI	TTATAA	1 cut(s)	2999
Psp124BI	GAGCTC	3 cut(s)	41, 962, 1806
Psp1406I	AACGTT	1 cut(s)	213
Psp6I	CCWGG	1 cut(s)	1984
PspFI	CCCAGC	2 cut(s)	281, 638
PspGI	CCWGG	1 cut(s)	1984
PspN4I	GGNNCC	3 cut(s)	1132, 1866, 2630
PspPI	GGNCC	2 cut(s)	1130, 1938
PsrI	GAACNNNNNNTAC	2 cut(s)	2930, 2962
PsuI	RGATCY	2 cut(s)	1297, 1737
RseI	CAYNNNNRTG	4 cut(s)	1460, 2861, 2867, 3101
SacI	GAGCTC	3 cut(s)	41, 962, 1806
SatI	GCNGC	4 cut(s)	2017, 2670, 2993, 3133
Sau3AI	GATC	8 cut(s)	88, 1248, 1297, 1402, 1452, 1737, 2382, 2611
Sau96I	GGNCC	2 cut(s)	1130, 1938
SchI	GAGTC	1 cut(s)	1983
ScrFI	CCNGG	4 cut(s)	263, 264, 1986, 2551
SduI	GDGCHC	6 cut(s)	41, 847, 962, 1335, 1806, 2539
SfuI	TTCGAA	1 cut(s)	3046
SmaI	CCCGGG	1 cut(s)	264
SmiMI	CAYNNNNRTG	4 cut(s)	1460, 2861, 2867, 3101
SmlI	CTYRAG	4 cut(s)	1106, 1267, 1610, 2752
SmoI	CTYRAG	4 cut(s)	1106, 1267, 1610, 2752
SphI	GCATGC	1 cut(s)	2023
SspI	AATATT	2 cut(s)	1225, 1512
SspMI	CTAG	3 cut(s)	1295, 1791, 2277
SstI	GAGCTC	3 cut(s)	41, 962, 1806
StyD4I	CCNGG	4 cut(s)	261, 262, 1984, 2549
StyI	CCWWGG	3 cut(s)	984, 1461, 2679
TaaI	ACNGT	8 cut(s)	103, 313, 741, 849, 1164, 2719, 2824, 3100
TaiI	ACGT	6 cut(s)	78, 216, 844, 1332, 2426, 2939
TaqI	TCGA	8 cut(s)	35, 833, 2227, 2504, 2545, 2848, 2942, 3046
TatI	WGTACW	6 cut(s)	78, 337, 1808, 1853, 2526, 2635
TauI	GCSGC	1 cut(s)	3135
TfiI	GAWTC	8 cut(s)	503, 1018, 1074, 1319, 1606, 2480, 2506, 2687
TscAI	CASTG	3 cut(s)	852, 2679, 3103
TseI	GCWGC	3 cut(s)	2016, 2669, 2992
TspGWI	ACGGA	3 cut(s)	486, 1832, 2375
TspMI	CCCGGG	1 cut(s)	262
TspRI	CASTG	3 cut(s)	852, 2679, 3103
Vha464I	CTTAAG	1 cut(s)	1267
VneI	GTGCAC	1 cut(s)	843
XceI	RCATGY	2 cut(s)	1630, 2023
XcmI	CCANNNNNNNNNTGG	1 cut(s)	555
XmaI	CCCGGG	1 cut(s)	262
XmiI	GTMKAC	3 cut(s)	2352, 2618, 2815
XmnI	GAANNNNTTC	5 cut(s)	1055, 1095, 1385, 2132, 2938
XspI	CTAG	3 cut(s)	1295, 1791, 2277
Zsp2I	ATGCAT	1 cut(s)	2025

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.