Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0007077

Physical Location & Seq

Genomic Coordinates Forward (+)

27727584 .. 27732217

Gene Structure 4634 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023746

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3279 bp

ATGGAAATTGAAGGGGAAGAGTATGAAGAGAGGGCTTGGTCAACGATGGATCCAGAACAACAAATGGTTTTGGGAGGAAGAGGAGCTCTTGGTGAGAATATTCAAAGGGGAGAAGAGAGAGAATGTGGACGTGCTGGTCTCGGTGATGAGACGAAAGAGAATTTTAAAGCAATAGGCATGCGAGGCTTATCCGCCAAATTACAGTCCTTGTGTTGGAGCTCAACCTCTAAAACCCTTCATTTCCGATTCTCAAAATTCCATAAGATTGATAACTTTGCGACCCTCACTAAGCCATCTAAGCCCAACAAAGCCCGCTTGAAGCAACAAGAAGAAGAACCCAGCTCGTTTGGATCGCTGTTCAGTGAAATAACTGAGATTTTAGGGGCTGAACATGTCACTCTGGATAAAACCCCATCTGGGTTTTTTATTTGCAAGGAAACCCATCTTGGGGTTGGTGAGGTTGGGCAAGAGAATGCTAAGGTGAATTCACTGGGTGAAAAGGAAGATATAGCTGTTGTGGAGGAAACCAGATTCGGAAGCGTAGCTGAAACTGATGTTAGCCTGGTAGTTCATGAGGTTACGAAGATTGTCAGGGCGGAAAATGGTTTGGTTTCGATGGAGGAGAGGTTAGACGAGTTGGGTTTTCAGTTAGATTCAGAGATTGTTGACAAAGTTCTGAAGAGGTGCTTTAAAGTGCCACATTTGGCTTTAAGGTTCTTTGATTGGGTGAAGCTGAAAGAAGGATTCTGTCACACGACTAAGACTTATAATACTGTGCTGTGTATAGCTGGGGAGGCAAGAGAGTTTGGGCTGGCAGAGAAGTTGGTGGAGGAGATGGAAAGGAACTCGTGCGAGAAGGATGTTAAGACCTGGACCATTCTTATCCAGATGTATGGGAAGGCTAAGTTAGTTGGAAGAGCGTTGTCAGTCTATGAGAAGATGAAGAAATGCGGTTTTGAACCAGACTCGGTGGTTTTCAGAATGATGATACGTGCACTTTGTGCTGCTGGAAAAGCCGACGTGGCAATGGAATTTTACAAGGAAATGATACAGGAGGACGTTCAACTTGATATCAATATGTATAAGCTGTTATTGAATGGCATAGCTAGCTATGGAGATACTGCTGCTGTGGGCTTGGTTTCAGATGACATGATGAGAGTCTCACAGATTCCTGAACACGTGGTTTATGGTAGTGTCCTGAAGTCTTTCTGTATCTCTGGTAGAATCAGAGAAGCTTTGGAATACATTCGTGAGCTCAAGAATAAAGATGTTATGCTTCTCCCTGAATATTTTGAGACTTTGGTGAAAGGACTGTGCAGGGCTGATAGAATTGCAGATGCATTGGAGATTTTGGATATTATGAAGAAAAGAGATTTTGTTGATGGAAATGTGTATGGGATTATCATCAATGGATACTTGAGAAAAAATGATGTTTCTAGAGCACTTGATCTGTTTCGTAGTATGAAAGAATGTGGATATTTGCCTACTACTTCTACCTACACTGAACTGATGCAGCACCTCTTCAATGTGAATGAGTATCAACAAGGCTGTGAGTTGTATGATGAGATGCTTGAAAGAGGAGTTGAGCCAGATAGTGTGGCCGTTACAGCCATGGTTGCAGGCCACGTCCGCCAAAACCATATATCTGAAGCATGGAAAGTATTTAATGATATGAAGGACAAAGGCATTAAGCCCACTTCAAAGTTGTATTTAGTATTTATCAAGGAGCTTTGTAGCATTTCAAAGACAGATGAGATTATGAAGGTTTTGGTTCATATGCGGGCATCTGATATGCGCATTAAAGATGACATATTCAATTGGGTTGTACTTCATATGGAGAAAAAGGGAGAGATGGATAGCATTGAAAAAGTAAAGCAGATGCAAAGTCTTTATAAATTGCAGCCTCATGATGGAGAGGTATCAAGTAGTGATGCATCTAGTGGAGAGAGGACCAATACAGTGTTGGAGTTGCTACCAAAGAAGATGGATCCCTTATTGAATTCTGATGCTTATGATGACAAGGATTTGCAAGAAATTTGTAAAATTCTGTCCTCTTCAACTGACTGGTGCTTAATTCAAGAAGATTTGGAGAAGTCTGCTGTTGATTACACTCCAGGACTCGTTCTGGAAATTTTGAGGAATCACAGTGGGCATGGTTCGGTAGCGTTACAGTTTTTCTCATGGGTAGGAAAGCAATCTGGTTATTGCCACACTACTGATACTTACAACATGGCTATCAAAATTGCAGGGCGTGGGAAAGATTTCAAGCACATGAGGAACCTTTTCTATGAAATGAGGAGAAACAGTTTCTCAATAACACAAGATACATGGACAATAATGATAATGCAATATGGTCGAACAGGTCTCACAGAGATTGCTCTTCAGTTTTTCAGAGAGATGAAAAGTAATTGCAACCCAACTGGCAGTACCTATAAGTATTTGATCATATCTCTTTGTGGGAGAAAAGGTCGAAGGGTAGATGAAGCAATTAAAATATTCCAGGAGATGATCCGTGCTGCACATATACCTGACAAAGAGTTGGTTGAAACTTATGTTGGCTGTTTATGTGAAGTTGGTAAGCTGTCAGATGCTAGAAGATGCATAGATTCTCTTTCCGAAGTTGGTTTTACCACCTCTCTTGGTTGTTCCTTGTACATTAGGGCTCTTTGTCGAGCTGGGAAGTTACAAGAAGCTTCTGCGCTGATGGATGATATTGGGGAAGAGCGATCCACATTGGATCGGTATACTTATGGGAGCTTCGTTCATGGACTTTTGCGGAGTGGACAAGTAGAAGAAGCACTGGCCAAGGTGGAGTCTATGAAGCAGGCAGGCGTGAAGCCAACCGTCCATGTTTATACATCCTTAATAGTTCATTTCTTTAAGGAGAAGCAGATCAAAAGGGCATTAGAAATTTTCAATGAAATGCAACAGGAAGGATGTGAACCGACGGTTGTTACTTATTCCGCGCTTATCCGAGGGTACATGAACATGGAGATGATTGCTGAAGCTTGGAGTGTCTTTCACAGCATGAAACTGAAAGGGCCATTGCCGGACTTCAAAACTTATTCAATGTTCCTTCATTGTCTTTGTAAGGCAGGGAAATCAGAAGAAGCCATGCAGCTTATATCTGAAATGTTGAACAGTGGGATTGTTCCCAGTGTTGTCAATTTTCGAACTGTATTTTACGGACTTAACCGAGAAGGTAAGCAAGATTTGGCTCGCACTGTAATGGAACGGAAGTCATCTTTGATTAGAAAACGTAAGTTCTTAATATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1093

Amino Acids

123.98

Weight (kDa)

6.09

Isoelectric Point (pI)

28.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	253 - 299	2.2e-07	PPR repeat family
PPR_2	PF13041	286 - 334	2.6e-12	PPR repeat family
PPR	PF01535	290 - 319	4.6e-06	PPR repeat
PPR_1	PF12854	318 - 349	2.5e-06	PPR repeat
PPR_2	PF13041	321 - 369	1e-06	PPR repeat family
PPR_2	PF13041	465 - 504	2e-08	PPR repeat family
PPR_long	PF17177	474 - 603	7.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	495 - 541	7e-09	PPR repeat family
PPR_2	PF13041	530 - 578	6.6e-12	PPR repeat family
PPR_2	PF13041	776 - 819	1.3e-06	PPR repeat family
TPR_24	PF23276	891 - 1009	9.7e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	915 - 956	1.3e-06	PPR repeat family
PPR_long	PF17177	926 - 1079	1.4e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	938 - 994	6.8e-14	Pentatricopeptide repeat domain
PPR_2	PF13041	947 - 995	3.5e-15	PPR repeat family
TPR_24	PF23276	950 - 1041	1.8e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	979 - 1011	6.3e-06	PPR repeat
PPR_2	PF13041	982 - 1031	2.3e-13	PPR repeat family
PPR_1	PF12854	1013 - 1045	2.7e-07	PPR repeat
PPR	PF01535	1021 - 1050	9.5e-06	PPR repeat
PPR_2	PF13041	1021 - 1064	1.1e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06400
fragaria_vesca	FvH4_6g20810 FvH4_6g20810 FvH4_6g20810
malus_domestica	MD12G1044100.v1.1
prunus_persica	Prupe.7G091000_v2.0.a1
pyrus_communis	pycom12g03800
rosa_chinensis	RchiOBHm_Chr3g0476881
rosa_laevigata	RLG00000023746
rosa_multiflora	Rmu_sc0000288.1_g000033
rosa_roxburghii	Rroxscaffold_6G00403680 Rroxscaffold_6G00404930
rosa_rugosa	Rorug03G0157400
rosa_samantha	Rh3AG207600 Rh3AG229900 Rh3BG240200 Rh3BG241300 Rh3CG233900 Rh3CG235000 Rh3CG235700 Rh3CG259900 Rh3DG233900
rosa_wichuraiana	Rw0G011140 Rw3G018900 Rw3G020670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	768, 1893
AasI	GACNNNNNNGTC	1 cut(s)	135
Acc16I	TGCGCA	1 cut(s)	1796
AccI	GTMKAC	1 cut(s)	2744
AccII	CGCG	1 cut(s)	2966
AciI	CCGC	8 cut(s)	192, 313, 596, 951, 1630, 1780, 2776, 2964
AclWI	GGATC	8 cut(s)	44, 57, 358, 1982, 1995, 2503, 2721, 2745
AcoI	YGGCCR	2 cut(s)	1599, 2802
AcsI	RAATTY	9 cut(s)	160, 254, 484, 1031, 1999, 2034, 2043, 2130, 2910
AcuI	CTGAAG	5 cut(s)	698, 1220, 1668, 2366, 3024
AcvI	CACGTG	1 cut(s)	1180
AdeI	CACNNNGTG	2 cut(s)	494, 1001
AfaI	GTAC	4 cut(s)	1827, 2428, 2654, 2981
AfiI	CCNNNNNNNGG	5 cut(s)	213, 417, 447, 448, 1910
AflIII	ACRYGT	2 cut(s)	391, 1177
AjiI	CACGTC	3 cut(s)	131, 1021, 1627
AjnI	CCWGG	4 cut(s)	561, 869, 2113, 2499
AjuI	GAANNNNNNNTTGG	4 cut(s)	429, 461, 2538, 2570
Alw21I	GWGCWC	5 cut(s)	88, 221, 997, 1257, 1444
Alw26I	GTCTC	5 cut(s)	143, 143, 1165, 1289, 2369
Alw44I	GTGCAC	1 cut(s)	993
AlwI	GGATC	8 cut(s)	44, 57, 358, 1982, 1995, 2503, 2721, 2745
AoxI	GGCC	4 cut(s)	1599, 1621, 2802, 3041
ApaLI	GTGCAC	1 cut(s)	993
ApeKI	GCWGC	6 cut(s)	1004, 1124, 1513, 1900, 2516, 3118
ApoI	RAATTY	9 cut(s)	160, 254, 484, 1031, 1999, 2034, 2043, 2130, 2910
Asp700I	GAANNNNTTC	4 cut(s)	1205, 1245, 2261, 2282
AspLEI	GCGC	3 cut(s)	1797, 2701, 2968
AspS9I	GGNCC	3 cut(s)	873, 1950, 3041
AsuHPI	GGTGA	7 cut(s)	104, 155, 467, 493, 506, 739, 1315
AsuII	TTCGAA	1 cut(s)	3172
AsuNHI	GCTAGC	1 cut(s)	1106
AvaII	GGWCC	2 cut(s)	873, 1950
BaeGI	GKGCMC	1 cut(s)	997
BalI	TGGCCA	1 cut(s)	2804
BamHI	GGATCC	2 cut(s)	49, 1987
BanII	GRGCYC	4 cut(s)	88, 221, 1257, 2665
BauI	CACGAG	1 cut(s)	847
BbrPI	CACGTG	1 cut(s)	1180
Bbv12I	GWGCWC	5 cut(s)	88, 221, 997, 1257, 1444
BbvI	GCAGC	6 cut(s)	991, 1111, 1525, 1912, 2503, 3130
BceAI	ACGGC	1 cut(s)	1586
BcgI	CGANNNNNNTGC	2 cut(s)	2336, 2370
BciT130I	CCWGG	4 cut(s)	563, 871, 2115, 2501
BciVI	GTATCC	1 cut(s)	1406
BclI	TGATCA	1 cut(s)	2442
BcoDI	GTCTC	5 cut(s)	143, 143, 1165, 1289, 2369
BfaI	CTAG	4 cut(s)	1107, 1437, 1938, 2592
BfuI	GTATCC	1 cut(s)	1406
BglI	GCCNNNNNGGC	1 cut(s)	1022
BisI	GCNGC	6 cut(s)	1005, 1125, 1514, 1901, 2517, 3119
BlsI	GCNGC	6 cut(s)	1006, 1126, 1515, 1902, 2518, 3120
Bme1390I	CCNGG	4 cut(s)	563, 871, 2115, 2501
Bme18I	GGWCC	2 cut(s)	873, 1950
BmgBI	CACGTC	3 cut(s)	131, 1021, 1627
BmgT120I	GGNCC	3 cut(s)	873, 1950, 3041
BmiI	GGNNCC	3 cut(s)	51, 1989, 2279
BmrFI	CCNGG	4 cut(s)	563, 871, 2115, 2501
BmrI	ACTGGG	2 cut(s)	500, 3150
BmtI	GCTAGC	1 cut(s)	1110
BmuI	ACTGGG	2 cut(s)	500, 3150
BpmI	CTGGAG	1 cut(s)	2097
Bpu10I	CCTNAGC	1 cut(s)	477
Bpu14I	TTCGAA	1 cut(s)	3172
BpuEI	CTTGAG	2 cut(s)	1241, 1438
BsaAI	YACGTR	2 cut(s)	992, 1180
BsaBI	GATNNNNATC	1 cut(s)	1403
BsaI	GGTCTC	2 cut(s)	143, 2369
BsaJI	CCNNGG	3 cut(s)	1611, 2805, 2974
BsaXI	ACNNNNNCTCC	6 cut(s)	611, 641, 2290, 2320, 2452, 2482
Bsc4I	CCNNNNNNNGG	5 cut(s)	213, 417, 447, 448, 1910
Bse1I	ACTGG	5 cut(s)	495, 2069, 2425, 2805, 3156
Bse3DI	GCAATG	2 cut(s)	1032, 3044
Bse8I	GATNNNNATC	1 cut(s)	1403
BseBI	CCWGG	4 cut(s)	563, 871, 2115, 2501
BseDI	CCNNGG	3 cut(s)	1611, 2805, 2974
BseGI	GGATG	4 cut(s)	865, 2713, 2858, 2942
BseJI	GATNNNNATC	1 cut(s)	1403
BseLI	CCNNNNNNNGG	5 cut(s)	213, 417, 447, 448, 1910
BseMI	GCAATG	2 cut(s)	1032, 3044
BseMII	CTCAG	1 cut(s)	363
BseNI	ACTGG	5 cut(s)	495, 2069, 2425, 2805, 3156
BseRI	GAGGAG	5 cut(s)	96, 635, 845, 1593, 2311
BseSI	GKGCMC	1 cut(s)	997
BseXI	GCAGC	6 cut(s)	991, 1111, 1525, 1912, 2503, 3130
BseYI	CCCAGC	3 cut(s)	338, 788, 2675
BsgI	GTGCAG	2 cut(s)	1336, 2502
Bsh1236I	CGCG	1 cut(s)	2966
BshFI	GGCC	4 cut(s)	1601, 1623, 2804, 3043
BsiHKAI	GWGCWC	5 cut(s)	88, 221, 997, 1257, 1444
BsiSI	CCGG	1 cut(s)	3050
BslI	CCNNNNNNNGG	5 cut(s)	213, 417, 447, 448, 1910
BsmAI	GTCTC	5 cut(s)	143, 143, 1165, 1289, 2369
BsmBI	CGTCTC	1 cut(s)	143
BsmI	GAATGC	1 cut(s)	478
BsnI	GGCC	4 cut(s)	1601, 1623, 2804, 3043
Bso31I	GGTCTC	2 cut(s)	143, 2369
Bsp119I	TTCGAA	1 cut(s)	3172
Bsp1286I	GDGCHC	6 cut(s)	88, 221, 997, 1257, 1444, 2665
Bsp1407I	TGTACA	1 cut(s)	2652
Bsp143I	GATC	9 cut(s)	49, 350, 1447, 1987, 2442, 2508, 2726, 2737, 2892
Bsp19I	CCATGG	1 cut(s)	1611
BspACI	CCGC	8 cut(s)	192, 313, 596, 951, 1630, 1780, 2776, 2964
BspANI	GGCC	4 cut(s)	1601, 1623, 2804, 3043
BspCNI	CTCAG	1 cut(s)	364
BspFNI	CGCG	1 cut(s)	2966
BspHI	TCATGA	3 cut(s)	571, 1906, 3275
BspLI	GGNNCC	3 cut(s)	51, 1989, 2279
BspOI	GCTAGC	1 cut(s)	1110
BspPI	GGATC	8 cut(s)	44, 57, 358, 1982, 1995, 2503, 2721, 2745
BspQI	GCTCTTC	3 cut(s)	910, 2385, 2715
BspT104I	TTCGAA	1 cut(s)	3172
BspTNI	GGTCTC	2 cut(s)	143, 2369
BsrDI	GCAATG	2 cut(s)	1032, 3044
BsrGI	TGTACA	1 cut(s)	2652
BsrI	ACTGG	5 cut(s)	495, 2069, 2425, 2805, 3156
BssECI	CCNNGG	3 cut(s)	1611, 2805, 2974
BssMI	GATC	9 cut(s)	49, 350, 1447, 1987, 2442, 2508, 2726, 2737, 2892
BssNAI	GTATAC	1 cut(s)	2745
BssSI	CACGAG	1 cut(s)	847
BssT1I	CCWWGG	2 cut(s)	1611, 2805
Bst1107I	GTATAC	1 cut(s)	2745
Bst2BI	CACGAG	1 cut(s)	847
Bst2UI	CCWGG	4 cut(s)	563, 871, 2115, 2501
BstAPI	GCANNNNNTGC	1 cut(s)	1001
BstAUI	TGTACA	1 cut(s)	2652
BstBAI	YACGTR	2 cut(s)	992, 1180
BstBI	TTCGAA	1 cut(s)	3172
BstC8I	GCNNGC	9 cut(s)	179, 313, 813, 1108, 1621, 1782, 2826, 2830, 3220
BstDEI	CTNAG	6 cut(s)	288, 297, 372, 477, 759, 903
BstDSI	CCRYGG	1 cut(s)	1611
BstF5I	GGATG	4 cut(s)	865, 2713, 2858, 2942
BstFNI	CGCG	1 cut(s)	2966
BstHHI	GCGC	3 cut(s)	1797, 2701, 2968
BstKTI	GATC	9 cut(s)	52, 353, 1450, 1990, 2445, 2511, 2729, 2740, 2895
BstMAI	GTCTC	5 cut(s)	143, 143, 1165, 1289, 2369
BstMBI	GATC	9 cut(s)	49, 350, 1447, 1987, 2442, 2508, 2726, 2737, 2892
BstNI	CCWGG	4 cut(s)	563, 871, 2115, 2501
BstNSI	RCATGY	2 cut(s)	181, 395
BstSCI	CCNGG	4 cut(s)	561, 869, 2113, 2499
BstSLI	GKGCMC	1 cut(s)	997
BstUI	CGCG	1 cut(s)	2966
BstV1I	GCAGC	6 cut(s)	991, 1111, 1525, 1912, 2503, 3130
BstX2I	RGATCY	2 cut(s)	49, 1987
BstXI	CCANNNNNNTGG	1 cut(s)	893
BstYI	RGATCY	2 cut(s)	49, 1987
BstZ17I	GTATAC	1 cut(s)	2745
BsuI	GTATCC	1 cut(s)	1406
BsuRI	GGCC	4 cut(s)	1601, 1623, 2804, 3043
BtgI	CCRYGG	1 cut(s)	1611
BtrI	CACGTC	3 cut(s)	131, 1021, 1627
BtsCI	GGATG	4 cut(s)	865, 2713, 2858, 2942
BtsIMutI	CAGTG	9 cut(s)	367, 488, 1500, 1965, 2152, 2798, 3148, 3163, 3222
Cac8I	GCNNGC	9 cut(s)	179, 313, 813, 1108, 1621, 1782, 2826, 2830, 3220
CciI	TCATGA	3 cut(s)	571, 1906, 3275
CfoI	GCGC	3 cut(s)	1797, 2701, 2968
Cfr13I	GGNCC	3 cut(s)	873, 1950, 3041
Csp6I	GTAC	4 cut(s)	1826, 2427, 2653, 2980
CspCI	CAANNNNNGTGG	2 cut(s)	2620, 2655
CviQI	GTAC	4 cut(s)	1826, 2427, 2653, 2980
DdeI	CTNAG	6 cut(s)	288, 297, 372, 477, 759, 903
DpnI	GATC	9 cut(s)	51, 352, 1449, 1989, 2444, 2510, 2728, 2739, 2894
DpnII	GATC	9 cut(s)	49, 350, 1447, 1987, 2442, 2508, 2726, 2737, 2892
DraI	TTTAAA	2 cut(s)	166, 691
DraIII	CACNNNGTG	2 cut(s)	494, 1001
DrdI	GACNNNNNNGTC	1 cut(s)	135
DseDI	GACNNNNNNGTC	1 cut(s)	135
EaeI	YGGCCR	2 cut(s)	1599, 2802
EciI	GGCGGA	3 cut(s)	181, 611, 1619
Ecl136II	GAGCTC	3 cut(s)	86, 219, 1255
Eco130I	CCWWGG	2 cut(s)	1611, 2805
Eco24I	GRGCYC	4 cut(s)	88, 221, 1257, 2665
Eco31I	GGTCTC	2 cut(s)	143, 2369
Eco32I	GATATC	1 cut(s)	1072
Eco47I	GGWCC	2 cut(s)	873, 1950
Eco53kI	GAGCTC	3 cut(s)	86, 219, 1255
Eco57I	CTGAAG	5 cut(s)	698, 1220, 1668, 2366, 3024
Eco72I	CACGTG	1 cut(s)	1180
EcoICRI	GAGCTC	3 cut(s)	86, 219, 1255
EcoRI	GAATTC	2 cut(s)	484, 1999
EcoRII	CCWGG	4 cut(s)	561, 869, 2113, 2499
EcoRV	GATATC	1 cut(s)	1072
EcoT14I	CCWWGG	2 cut(s)	1611, 2805
EcoT22I	ATGCAT	3 cut(s)	1342, 1936, 2603
EcoT38I	GRGCYC	4 cut(s)	88, 221, 1257, 2665
ErhI	CCWWGG	2 cut(s)	1611, 2805
Esp3I	CGTCTC	1 cut(s)	143
FalI	AAGNNNNNCTT	2 cut(s)	671, 703
FauI	CCCGC	2 cut(s)	320, 1773
FauNDI	CATATG	2 cut(s)	1776, 1833
FbaI	TGATCA	1 cut(s)	2442
FblI	GTMKAC	1 cut(s)	2744
Fnu4HI	GCNGC	6 cut(s)	1005, 1125, 1514, 1901, 2517, 3119
FokI	GGATG	4 cut(s)	872, 2720, 2845, 2949
FriOI	GRGCYC	4 cut(s)	88, 221, 1257, 2665
Fsp4HI	GCNGC	6 cut(s)	1005, 1125, 1514, 1901, 2517, 3119
FspAI	RTGCGCAY	1 cut(s)	1796
FspBI	CTAG	4 cut(s)	1107, 1437, 1938, 2592
FspI	TGCGCA	1 cut(s)	1796
GlaI	GCGC	3 cut(s)	1796, 2700, 2967
GluI	GCNGC	6 cut(s)	1005, 1125, 1514, 1901, 2517, 3119
GsaI	CCCAGC	3 cut(s)	342, 792, 2679
GsuI	CTGGAG	1 cut(s)	2097
HaeIII	GGCC	4 cut(s)	1601, 1623, 2804, 3043
HapII	CCGG	1 cut(s)	3050
HhaI	GCGC	3 cut(s)	1797, 2701, 2968
Hin6I	GCGC	3 cut(s)	1795, 2699, 2966
HinP1I	GCGC	3 cut(s)	1795, 2699, 2966
HincII	GTYRAC	2 cut(s)	42, 667
HindII	GTYRAC	2 cut(s)	42, 667
HindIII	AAGCTT	3 cut(s)	1233, 2691, 3006
HpaII	CCGG	1 cut(s)	3050
HphI	GGTGA	7 cut(s)	104, 155, 467, 493, 506, 739, 1315
Hpy166II	GTNNAC	7 cut(s)	42, 128, 667, 995, 2745, 2783, 2942
Hpy8I	GTNNAC	7 cut(s)	42, 128, 667, 995, 2745, 2783, 2942
Hpy99I	CGWCG	2 cut(s)	1022, 2950
HpyCH4IV	ACGT	7 cut(s)	130, 991, 1020, 1059, 1179, 1626, 3259
HpyF3I	CTNAG	6 cut(s)	288, 297, 372, 477, 759, 903
HpySE526I	ACGT	7 cut(s)	130, 991, 1020, 1059, 1179, 1626, 3259
HspAI	GCGC	3 cut(s)	1795, 2699, 2966
Ksp22I	TGATCA	1 cut(s)	2442
Kzo9I	GATC	9 cut(s)	49, 350, 1447, 1987, 2442, 2508, 2726, 2737, 2892
LguI	GCTCTTC	3 cut(s)	910, 2385, 2715
LmnI	GCTCC	4 cut(s)	83, 216, 1726, 2754
Lsp1109I	GCAGC	6 cut(s)	991, 1111, 1525, 1912, 2503, 3130
MaeI	CTAG	4 cut(s)	1107, 1437, 1938, 2592
MaeII	ACGT	7 cut(s)	130, 991, 1020, 1059, 1179, 1626, 3259
MaeIII	GTNAC	7 cut(s)	394, 577, 749, 1603, 2166, 2682, 2953
MalI	GATC	9 cut(s)	51, 352, 1449, 1989, 2444, 2510, 2728, 2739, 2894
MboI	GATC	9 cut(s)	49, 350, 1447, 1987, 2442, 2508, 2726, 2737, 2892
MfeI	CAATTG	1 cut(s)	1816
MflI	RGATCY	2 cut(s)	49, 1987
MhlI	GDGCHC	6 cut(s)	88, 221, 997, 1257, 1444, 2665
MlsI	TGGCCA	1 cut(s)	2804
MluNI	TGGCCA	1 cut(s)	2804
MlyI	GAGTC	4 cut(s)	959, 1167, 2112, 2822
MmeI	TCCRAC	3 cut(s)	194, 892, 1944
Mox20I	TGGCCA	1 cut(s)	2804
Mph1103I	ATGCAT	3 cut(s)	1342, 1936, 2603
MroXI	GAANNNNTTC	4 cut(s)	1205, 1245, 2261, 2282
MscI	TGGCCA	1 cut(s)	2804
MslI	CAYNNNNRTG	3 cut(s)	2987, 2993, 3227
Msp20I	TGGCCA	1 cut(s)	2804
MspI	CCGG	1 cut(s)	3050
MspR9I	CCNGG	4 cut(s)	563, 871, 2115, 2501
MunI	CAATTG	1 cut(s)	1816
Mva1269I	GAATGC	1 cut(s)	478
MvaI	CCWGG	4 cut(s)	563, 871, 2115, 2501
MvnI	CGCG	1 cut(s)	2966
NcoI	CCATGG	1 cut(s)	1611
NdeI	CATATG	2 cut(s)	1776, 1833
NdeII	GATC	9 cut(s)	49, 350, 1447, 1987, 2442, 2508, 2726, 2737, 2892
NheI	GCTAGC	1 cut(s)	1106
NlaIV	GGNNCC	3 cut(s)	51, 1989, 2279
NmuCI	GTSAC	2 cut(s)	394, 749
NsbI	TGCGCA	1 cut(s)	1796
NsiI	ATGCAT	3 cut(s)	1342, 1936, 2603
NspI	RCATGY	2 cut(s)	181, 395
NspV	TTCGAA	1 cut(s)	3172
PaeI	GCATGC	1 cut(s)	181
PagI	TCATGA	3 cut(s)	571, 1906, 3275
PciI	ACATGT	1 cut(s)	391
PciSI	GCTCTTC	3 cut(s)	910, 2385, 2715
PctI	GAATGC	1 cut(s)	478
PdmI	GAANNNNTTC	4 cut(s)	1205, 1245, 2261, 2282
PfeI	GAWTC	8 cut(s)	246, 531, 653, 744, 1168, 1224, 2140, 2606
PfoI	TCCNGGA	2 cut(s)	2113, 2499
PkrI	GCNGC	6 cut(s)	1006, 1126, 1515, 1902, 2518, 3120
PleI	GAGTC	4 cut(s)	959, 1166, 2112, 2821
PmaCI	CACGTG	1 cut(s)	1180
PmlI	CACGTG	1 cut(s)	1180
PpsI	GAGTC	4 cut(s)	959, 1166, 2112, 2821
Ppu21I	YACGTR	2 cut(s)	992, 1180
PscI	ACATGT	1 cut(s)	391
PsiI	TTATAA	2 cut(s)	768, 1893
Psp124BI	GAGCTC	3 cut(s)	88, 221, 1257
Psp6I	CCWGG	4 cut(s)	561, 869, 2113, 2499
PspCI	CACGTG	1 cut(s)	1180
PspFI	CCCAGC	3 cut(s)	338, 788, 2675
PspGI	CCWGG	4 cut(s)	561, 869, 2113, 2499
PspN4I	GGNNCC	3 cut(s)	51, 1989, 2279
PspPI	GGNCC	3 cut(s)	873, 1950, 3041
PsuI	RGATCY	2 cut(s)	49, 1987
RsaI	GTAC	4 cut(s)	1827, 2428, 2654, 2981
RsaNI	GTAC	4 cut(s)	1826, 2427, 2653, 2980
RseI	CAYNNNNRTG	3 cut(s)	2987, 2993, 3227
SacI	GAGCTC	3 cut(s)	88, 221, 1257
SapI	GCTCTTC	3 cut(s)	910, 2385, 2715
SatI	GCNGC	6 cut(s)	1005, 1125, 1514, 1901, 2517, 3119
Sau3AI	GATC	9 cut(s)	49, 350, 1447, 1987, 2442, 2508, 2726, 2737, 2892
Sau96I	GGNCC	3 cut(s)	873, 1950, 3041
SchI	GAGTC	4 cut(s)	959, 1167, 2112, 2822
ScrFI	CCNGG	4 cut(s)	563, 871, 2115, 2501
SduI	GDGCHC	6 cut(s)	88, 221, 997, 1257, 1444, 2665
SfuI	TTCGAA	1 cut(s)	3172
SinI	GGWCC	2 cut(s)	873, 1950
SmiMI	CAYNNNNRTG	3 cut(s)	2987, 2993, 3227
SmlI	CTYRAG	2 cut(s)	1256, 1417
SmoI	CTYRAG	2 cut(s)	1256, 1417
SphI	GCATGC	1 cut(s)	181
SsiI	CCGC	8 cut(s)	192, 313, 596, 951, 1630, 1780, 2776, 2964
SspI	AATATT	3 cut(s)	100, 1289, 2496
SspMI	CTAG	4 cut(s)	1107, 1437, 1938, 2592
SstI	GAGCTC	3 cut(s)	88, 221, 1257
StyD4I	CCNGG	4 cut(s)	561, 869, 2113, 2499
StyI	CCWWGG	2 cut(s)	1611, 2805
TaiI	ACGT	7 cut(s)	133, 994, 1023, 1062, 1182, 1629, 3262
TaqI	TCGA	5 cut(s)	614, 2356, 2470, 2671, 3172
TatI	WGTACW	2 cut(s)	1825, 2652
TfiI	GAWTC	8 cut(s)	246, 531, 653, 744, 1168, 1224, 2140, 2606
TscAI	CASTG	9 cut(s)	367, 495, 1507, 1965, 2152, 2805, 3148, 3163, 3229
TseFI	GTSAC	2 cut(s)	394, 749
TseI	GCWGC	6 cut(s)	1004, 1124, 1513, 1900, 2516, 3118
Tsp45I	GTSAC	2 cut(s)	394, 749
TspGWI	ACGGA	3 cut(s)	2501, 3201, 3250
TspRI	CASTG	9 cut(s)	367, 495, 1507, 1965, 2152, 2805, 3148, 3163, 3229
VneI	GTGCAC	1 cut(s)	993
VpaK11BI	GGWCC	2 cut(s)	873, 1950
XapI	RAATTY	9 cut(s)	160, 254, 484, 1031, 1999, 2034, 2043, 2130, 2910
XbaI	TCTAGA	1 cut(s)	1436
XceI	RCATGY	2 cut(s)	181, 395
XcmI	CCANNNNNNNNNTGG	2 cut(s)	449, 1960
XmiI	GTMKAC	1 cut(s)	2744
XmnI	GAANNNNTTC	4 cut(s)	1205, 1245, 2261, 2282
XspI	CTAG	4 cut(s)	1107, 1437, 1938, 2592
Zsp2I	ATGCAT	3 cut(s)	1342, 1936, 2603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.