FvH4_6g21250

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007298
Physical Location & Seq
Forward (+)
14804828 .. 14806861
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21250.t1

Sequence Viewer

Length: 774 bp
ATGTCTAAAACCAAAAGCAAGAATCATCATCTCTATCTTCAAAACCCTGAAAACCACGTGAAGGAGCCCTCAGAAAAACAAACCCAGAATCCATTTGTCACAAACTCACCCCATTTATATACCACCAGAAACCAACCTGTTCGACCACACCAATCTCCAATGGAGTACACATACGACGCAGGTAGCGGTACCACAGAGCAGACTGCCCCATTGGTACACGGTGGGGCTGAGGGACTGAGAAGAACGAAGATGAGGGTGATGGTCGCCATCGATGAAAGTGACTCGAGCTTCTATGCTCTCACCTGGGCGCTTGACCATCTCTTTATCAACCCACCGAGCACGGTGGAAGGCGGCGAGGGGGAGGCCGGGTTGGTGACGGTGGTCCACGTCACGCAGCCGTTTCAGCACTTTGTGTACCCTGCTGGACCTGGCGGAGCAACCTATTATGCTTCTGCCTCGGTGGTGGAGTCGGTGAAGAAAGCTCAGGAGGAGAGCTCTGCTGCCATTCTCTCCCGCGCTTTGCAGATTTGCAAGGACAGGATGATCAAAGCAGAAACTCTGATTATGACTGGAGATCCAAAGGACGTGATTTGTCAAGCCACAGAGCAGATGCATGTAGATCTTCTTGTTGTAGGTAGTCGTGGCCTGGGCATGGTTAAGAGGGCATTCCTAGGAAGTGTGAGCGACTATTGCGCACATCATGCAGCATGTCCAGTCGTGATCGTGAAGCCACCAAGGGGAACCGGATCAAAGGGCTCTGCATCTAAGAAGTAG

Protein Analysis

258

Amino Acids

27.87

Weight (kDa)

8.28

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 85 - 243 2.5e-27 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 694
Acc36I ACCTGC 1 cut(s) 170
Acc65I GGTACC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 188
AccII CGCG 1 cut(s) 516
AciI CCGC 4 cut(s) 186, 351, 432, 514
AclWI GGATC 2 cut(s) 569, 754
AcvI CACGTG 1 cut(s) 58
AdeI CACNNNGTG 1 cut(s) 412
AfaI GTAC 4 cut(s) 167, 190, 216, 416
AfiI CCNNNNNNNGG 3 cut(s) 61, 652, 737
AgsI TTSAA 1 cut(s) 41
AjiI CACGTC 2 cut(s) 388, 586
AjnI CCWGG 3 cut(s) 302, 427, 645
AluBI AGCT 3 cut(s) 288, 482, 495
AluI AGCT 3 cut(s) 288, 482, 495
Alw21I GWGCWC 2 cut(s) 341, 497
AlwI GGATC 2 cut(s) 569, 754
Ama87I CYCGRG 1 cut(s) 283
AoxI GGCC 2 cut(s) 363, 643
ApeKI GCWGC 3 cut(s) 394, 500, 704
Asp718I GGTACC 1 cut(s) 188
AspA2I CCTAGG 1 cut(s) 670
AspLEI GCGC 3 cut(s) 310, 518, 695
AspS9I GGNCC 2 cut(s) 382, 425
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 5 cut(s) 99, 268, 292, 385, 484
AvaI CYCGRG 1 cut(s) 283
AvaII GGWCC 2 cut(s) 382, 425
AvrII CCTAGG 1 cut(s) 670
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 3 cut(s) 69, 497, 758
BbrPI CACGTG 1 cut(s) 58
Bbv12I GWGCWC 2 cut(s) 341, 497
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 3 cut(s) 406, 487, 716
BccI CCATC 3 cut(s) 253, 275, 324
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 3 cut(s) 304, 429, 647
BclI TGATCA 1 cut(s) 543
BcnI CCSGG 1 cut(s) 367
BfaI CTAG 1 cut(s) 671
BfoI RGCGCY 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 170
BglII AGATCT 1 cut(s) 619
BisI GCNGC 4 cut(s) 352, 395, 501, 705
BlnI CCTAGG 1 cut(s) 670
BlsI GCNGC 4 cut(s) 353, 396, 502, 706
Bme1390I CCNGG 4 cut(s) 304, 367, 429, 647
Bme18I GGWCC 2 cut(s) 382, 425
BmeT110I CYCGRG 1 cut(s) 283
BmgBI CACGTC 2 cut(s) 388, 586
BmgT120I GGNCC 2 cut(s) 382, 425
BmiI GGNNCC 3 cut(s) 66, 190, 742
BmrFI CCNGG 4 cut(s) 304, 367, 429, 647
BmsI GCATC 2 cut(s) 600, 770
BoxI GACNNNNGTC 1 cut(s) 380
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 2 cut(s) 228, 483
BpuMI CCSGG 1 cut(s) 367
Bsa29I ATCGAT 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 58
BsaJI CCNNGG 5 cut(s) 303, 456, 646, 670, 734
BsaWI WCCGGW 1 cut(s) 743
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 652, 737
Bse1I ACTGG 2 cut(s) 574, 713
BseBI CCWGG 3 cut(s) 304, 429, 647
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 5 cut(s) 303, 456, 646, 670, 734
BseGI GGATG 1 cut(s) 546
BseLI CCNNNNNNNGG 3 cut(s) 61, 652, 737
BseMII CTCAG 4 cut(s) 84, 219, 227, 497
BseNI ACTGG 2 cut(s) 574, 713
BseRI GAGGAG 1 cut(s) 503
BseXI GCAGC 3 cut(s) 406, 487, 716
Bsh1236I CGCG 1 cut(s) 516
BshFI GGCC 2 cut(s) 365, 645
BshNI GGYRCC 1 cut(s) 188
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 2 cut(s) 341, 497
BsiHKCI CYCGRG 1 cut(s) 283
BsiSI CCGG 2 cut(s) 366, 744
BslFI GGGAC 1 cut(s) 246
BslI CCNNNNNNNGG 3 cut(s) 61, 652, 737
BsmFI GGGAC 1 cut(s) 246
BsmI GAATGC 1 cut(s) 665
BsnI GGCC 2 cut(s) 365, 645
BsoBI CYCGRG 1 cut(s) 283
Bsp1286I GDGCHC 4 cut(s) 69, 341, 497, 758
Bsp143I GATC 5 cut(s) 543, 574, 619, 720, 746
BspACI CCGC 4 cut(s) 186, 351, 432, 514
BspANI GGCC 2 cut(s) 365, 645
BspCNI CTCAG 4 cut(s) 83, 220, 228, 496
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 1 cut(s) 516
BspLI GGNNCC 3 cut(s) 66, 190, 742
BspMI ACCTGC 1 cut(s) 170
BspPI GGATC 2 cut(s) 569, 754
BspT107I GGYRCC 1 cut(s) 188
BsrI ACTGG 2 cut(s) 574, 713
BssECI CCNNGG 5 cut(s) 303, 456, 646, 670, 734
BssMI GATC 5 cut(s) 543, 574, 619, 720, 746
BssT1I CCWWGG 2 cut(s) 670, 734
Bst2UI CCWGG 3 cut(s) 304, 429, 647
Bst4CI ACNGT 3 cut(s) 221, 343, 379
BstAPI GCANNNNNTGC 1 cut(s) 701
BstBAI YACGTR 1 cut(s) 58
BstDEI CTNAG 5 cut(s) 70, 228, 236, 483, 765
BstF5I GGATG 1 cut(s) 546
BstFNI CGCG 1 cut(s) 516
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 3 cut(s) 310, 518, 695
BstKTI GATC 5 cut(s) 546, 577, 622, 723, 749
BstMBI GATC 5 cut(s) 543, 574, 619, 720, 746
BstMWI GCNNNNNNNGC 3 cut(s) 403, 690, 701
BstNI CCWGG 3 cut(s) 304, 429, 647
BstNSI RCATGY 2 cut(s) 617, 711
BstPAI GACNNNNGTC 1 cut(s) 380
BstSCI CCNGG 4 cut(s) 302, 365, 427, 645
BstUI CGCG 1 cut(s) 516
BstV1I GCAGC 3 cut(s) 406, 487, 716
BstX2I RGATCY 2 cut(s) 574, 619
BstYI RGATCY 2 cut(s) 574, 619
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 2 cut(s) 365, 645
BsuTUI ATCGAT 1 cut(s) 270
BtrI CACGTC 2 cut(s) 388, 586
BtsCI GGATG 1 cut(s) 546
BveI ACCTGC 1 cut(s) 170
CfoI GCGC 3 cut(s) 310, 518, 695
Cfr13I GGNCC 2 cut(s) 382, 425
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 1 cut(s) 185
Csp6I GTAC 4 cut(s) 166, 189, 215, 415
CviAII CATG 4 cut(s) 614, 652, 701, 708
CviQI GTAC 4 cut(s) 166, 189, 215, 415
DdeI CTNAG 5 cut(s) 70, 228, 236, 483, 765
DpnI GATC 5 cut(s) 545, 576, 621, 722, 748
DpnII GATC 5 cut(s) 543, 574, 619, 720, 746
DraIII CACNNNGTG 1 cut(s) 412
EciI GGCGGA 1 cut(s) 447
Ecl136II GAGCTC 1 cut(s) 495
Eco130I CCWWGG 2 cut(s) 670, 734
Eco24I GRGCYC 3 cut(s) 69, 497, 758
Eco47I GGWCC 2 cut(s) 382, 425
Eco53kI GAGCTC 1 cut(s) 495
Eco72I CACGTG 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 283
EcoICRI GAGCTC 1 cut(s) 495
EcoRII CCWGG 3 cut(s) 302, 427, 645
EcoT14I CCWWGG 2 cut(s) 670, 734
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 3 cut(s) 69, 497, 758
ErhI CCWWGG 2 cut(s) 670, 734
FaeI CATG 4 cut(s) 617, 655, 704, 711
FaqI GGGAC 1 cut(s) 246
FatI CATG 4 cut(s) 613, 651, 700, 707
FauI CCCGC 1 cut(s) 521
FbaI TGATCA 1 cut(s) 543
Fnu4HI GCNGC 4 cut(s) 352, 395, 501, 705
FokI GGATG 1 cut(s) 553
FriOI GRGCYC 3 cut(s) 69, 497, 758
Fsp4HI GCNGC 4 cut(s) 352, 395, 501, 705
FspBI CTAG 1 cut(s) 671
FspI TGCGCA 1 cut(s) 694
GlaI GCGC 3 cut(s) 309, 517, 694
GluI GCNGC 4 cut(s) 352, 395, 501, 705
GsuI CTGGAG 1 cut(s) 591
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 2 cut(s) 365, 645
HapII CCGG 2 cut(s) 366, 744
HgaI GACGC 1 cut(s) 185
HhaI GCGC 3 cut(s) 310, 518, 695
Hin1II CATG 4 cut(s) 617, 655, 704, 711
Hin6I GCGC 3 cut(s) 308, 516, 693
HinP1I GCGC 3 cut(s) 308, 516, 693
HinfI GANTC 4 cut(s) 22, 88, 281, 467
HpaII CCGG 2 cut(s) 366, 744
HphI GGTGA 5 cut(s) 99, 268, 292, 385, 484
Hpy166II GTNNAC 4 cut(s) 168, 217, 385, 415
Hpy188I TCNGA 2 cut(s) 73, 561
Hpy188III TCNNGA 3 cut(s) 485, 718, 724
Hpy8I GTNNAC 4 cut(s) 168, 217, 385, 415
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 2 cut(s) 55, 341
HpyCH4III ACNGT 3 cut(s) 221, 343, 379
HpyCH4IV ACGT 3 cut(s) 57, 387, 585
HpyCH4V TGCA 5 cut(s) 523, 531, 613, 704, 761
HpyF10VI GCNNNNNNNGC 3 cut(s) 403, 690, 701
HpyF3I CTNAG 5 cut(s) 70, 228, 236, 483, 765
HpySE526I ACGT 3 cut(s) 57, 387, 585
Hsp92II CATG 4 cut(s) 617, 655, 704, 711
HspAI GCGC 3 cut(s) 308, 516, 693
KpnI GGTACC 1 cut(s) 192
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 5 cut(s) 543, 574, 619, 720, 746
LmnI GCTCC 2 cut(s) 64, 434
Lsp1109I GCAGC 3 cut(s) 406, 487, 716
LweI GCATC 2 cut(s) 600, 770
MaeI CTAG 1 cut(s) 671
MaeII ACGT 3 cut(s) 57, 387, 585
MaeIII GTNAC 4 cut(s) 97, 278, 373, 388
MalI GATC 5 cut(s) 545, 576, 621, 722, 748
MboI GATC 5 cut(s) 543, 574, 619, 720, 746
MboII GAAGA 5 cut(s) 29, 252, 259, 487, 614
MflI RGATCY 2 cut(s) 574, 619
MhlI GDGCHC 4 cut(s) 69, 341, 497, 758
MlyI GAGTC 2 cut(s) 275, 476
MnlI CCTC 8 cut(s) 79, 223, 246, 349, 355, 466, 481, 654
Mph1103I ATGCAT 1 cut(s) 615
MseI TTAA 1 cut(s) 657
MspI CCGG 2 cut(s) 366, 744
MspR9I CCNGG 4 cut(s) 304, 367, 429, 647
Mva1269I GAATGC 1 cut(s) 665
MvaI CCWGG 3 cut(s) 304, 429, 647
MvnI CGCG 1 cut(s) 516
MwoI GCNNNNNNNGC 3 cut(s) 403, 690, 701
NciI CCSGG 1 cut(s) 367
NdeII GATC 5 cut(s) 543, 574, 619, 720, 746
NlaIII CATG 4 cut(s) 617, 655, 704, 711
NlaIV GGNNCC 3 cut(s) 66, 190, 742
NmuCI GTSAC 4 cut(s) 97, 278, 373, 388
NsbI TGCGCA 1 cut(s) 694
NsiI ATGCAT 1 cut(s) 615
NspI RCATGY 2 cut(s) 617, 711
PaeR7I CTCGAG 1 cut(s) 283
PctI GAATGC 1 cut(s) 665
PfeI GAWTC 2 cut(s) 22, 88
PkrI GCNGC 4 cut(s) 353, 396, 502, 706
PleI GAGTC 2 cut(s) 275, 475
PmaCI CACGTG 1 cut(s) 58
PmlI CACGTG 1 cut(s) 58
PpsI GAGTC 2 cut(s) 275, 475
Ppu21I YACGTR 1 cut(s) 58
PshAI GACNNNNGTC 1 cut(s) 380
Psp124BI GAGCTC 1 cut(s) 497
Psp6I CCWGG 3 cut(s) 302, 427, 645
PspCI CACGTG 1 cut(s) 58
PspGI CCWGG 3 cut(s) 302, 427, 645
PspN4I GGNNCC 3 cut(s) 66, 190, 742
PspPI GGNCC 2 cut(s) 382, 425
PspXI VCTCGAGB 1 cut(s) 283
PsuI RGATCY 2 cut(s) 574, 619
RsaI GTAC 4 cut(s) 167, 190, 216, 416
RsaNI GTAC 4 cut(s) 166, 189, 215, 415
SacI GAGCTC 1 cut(s) 497
SaqAI TTAA 1 cut(s) 657
SatI GCNGC 4 cut(s) 352, 395, 501, 705
Sau3AI GATC 5 cut(s) 543, 574, 619, 720, 746
Sau96I GGNCC 2 cut(s) 382, 425
SchI GAGTC 2 cut(s) 275, 476
ScrFI CCNGG 4 cut(s) 304, 367, 429, 647
SduI GDGCHC 4 cut(s) 69, 341, 497, 758
SfaNI GCATC 2 cut(s) 600, 770
Sfr274I CTCGAG 1 cut(s) 283
SinI GGWCC 2 cut(s) 382, 425
SlaI CTCGAG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 283
SmoI CTYRAG 1 cut(s) 283
SsiI CCGC 4 cut(s) 186, 351, 432, 514
SspMI CTAG 1 cut(s) 671
SstI GAGCTC 1 cut(s) 497
StyD4I CCNGG 4 cut(s) 302, 365, 427, 645
StyI CCWWGG 2 cut(s) 670, 734
TaaI ACNGT 3 cut(s) 221, 343, 379
TaiI ACGT 3 cut(s) 60, 390, 588
TaqI TCGA 3 cut(s) 142, 270, 284
TatI WGTACW 1 cut(s) 165
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 2 cut(s) 22, 88
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TseFI GTSAC 4 cut(s) 97, 278, 373, 388
TseI GCWGC 3 cut(s) 394, 500, 704
Tsp45I GTSAC 4 cut(s) 97, 278, 373, 388
TspDTI ATGAA 1 cut(s) 288
VpaK11BI GGWCC 2 cut(s) 382, 425
XceI RCATGY 2 cut(s) 617, 711
XhoI CTCGAG 1 cut(s) 283
XmaJI CCTAGG 1 cut(s) 670
XspI CTAG 1 cut(s) 671
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.