Rw3G019290

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007298
Physical Location & Seq
Forward (+)
21863901 .. 21864923
1023 bp
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UTR
Exon/CDS
Intron
Rw3G019290.1

Sequence Viewer

Length: 576 bp
ATGGAGACCACATACGCAGCTCCCGGTACCACAGTGCAGACGACCCCATTGGTGCACGTTGGGGATGAGGGACTGAGAAGAACGAAGATGAGGGTGATGGTCGCCATCGACGAAAGCGACTCGAGCTTCTACGCGCTCAACTGGGCGCTTGACCACCTCTTTATCAACGCGCCGAGTACTCTGGAAGGCGGCGAGGGGGAAGGGGGGATGGTGAAGGTGGTCCACGTCACGCCGCCGTTTCAGCACTTTGTGTACCCTGCCGGACCTTCGGTGAAGAAAGCTCAGCAGGAGAATTCTGCTGCCATTCTCTCCCGCGCTATGCAGATTTGCAAGGATAGGATGATCAAAGCAGAAACTCTGACTCTGACTGGAGATCCAAAGGACATGATTTGTCAAGCCACAGAGCAGATGCATGTAGATCTTCTTGTTGTGGGTAGTCGTGGCCTGGGCATGGTCAAGAGAGCATTCCTAGGAAGTGTGAGCGACTACTGCGCACATCATGCATCATGTCCAGTCATGATTGTAAAGCCACCAAGGGGGTCTGGAGCAAAGGGTTCTGCATCATCTAAAAAGTAG

Protein Analysis

191

Amino Acids

20.49

Weight (kDa)

8.35

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 31 - 176 1.5e-30 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 493
Acc65I GGTACC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 26
AccII CGCG 3 cut(s) 134, 170, 315
AciI CCGC 3 cut(s) 189, 233, 313
AclWI GGATC 1 cut(s) 368
AcsI RAATTY 1 cut(s) 292
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 3 cut(s) 28, 178, 254
AfiI CCNNNNNNNGG 2 cut(s) 451, 536
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 444
AluBI AGCT 3 cut(s) 20, 126, 281
AluI AGCT 3 cut(s) 20, 126, 281
Alw21I GWGCWC 1 cut(s) 57
Alw44I GTGCAC 1 cut(s) 53
AlwI GGATC 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 1 cut(s) 442
ApaLI GTGCAC 1 cut(s) 53
ApeKI GCWGC 2 cut(s) 17, 299
ApoI RAATTY 1 cut(s) 292
Asp718I GGTACC 1 cut(s) 26
AspA2I CCTAGG 1 cut(s) 469
AspLEI GCGC 5 cut(s) 136, 148, 172, 317, 494
AspS9I GGNCC 2 cut(s) 220, 263
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 3 cut(s) 106, 223, 283
AvaI CYCGRG 1 cut(s) 121
AvaII GGWCC 2 cut(s) 220, 263
AvrII CCTAGG 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 57
BaeI ACNNNNGTAYC 2 cut(s) 18, 51
BanI GGYRCC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 2 cut(s) 29, 286
BccI CCATC 3 cut(s) 91, 113, 202
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 446
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 24
BfaI CTAG 1 cut(s) 470
BfoI RGCGCY 1 cut(s) 149
BglII AGATCT 1 cut(s) 418
BisI GCNGC 4 cut(s) 18, 190, 233, 300
BlnI CCTAGG 1 cut(s) 469
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 4 cut(s) 19, 191, 234, 301
BmcAI AGTACT 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 24, 446
Bme18I GGWCC 2 cut(s) 220, 263
BmeT110I CYCGRG 1 cut(s) 121
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 220, 263
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 2 cut(s) 24, 446
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 3 cut(s) 399, 512, 569
BmuI ACTGGG 1 cut(s) 151
BpmI CTGGAG 2 cut(s) 390, 564
Bpu1102I GCTNAGC 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 445, 469, 533
Bsc4I CCNNNNNNNGG 2 cut(s) 451, 536
Bse1I ACTGG 3 cut(s) 146, 373, 512
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 3 cut(s) 445, 469, 533
BseGI GGATG 3 cut(s) 70, 213, 345
BseLI CCNNNNNNNGG 2 cut(s) 451, 536
BseMII CTCAG 2 cut(s) 65, 296
BseNI ACTGG 3 cut(s) 146, 373, 512
BseSI GKGCMC 1 cut(s) 57
BseXI GCAGC 2 cut(s) 29, 286
BsgI GTGCAG 1 cut(s) 56
Bsh1236I CGCG 3 cut(s) 134, 170, 315
BshFI GGCC 1 cut(s) 444
BshNI GGYRCC 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 2 cut(s) 24, 261
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 2 cut(s) 451, 536
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 1 cut(s) 444
BsoBI CYCGRG 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 3 cut(s) 342, 373, 418
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 3 cut(s) 189, 233, 313
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 66, 295
BspFNI CGCG 3 cut(s) 134, 170, 315
BspHI TCATGA 1 cut(s) 516
BspLI GGNNCC 1 cut(s) 28
BspPI GGATC 1 cut(s) 368
BspT107I GGYRCC 1 cut(s) 26
BsrI ACTGG 3 cut(s) 146, 373, 512
BssECI CCNNGG 3 cut(s) 445, 469, 533
BssMI GATC 3 cut(s) 342, 373, 418
BssT1I CCWWGG 2 cut(s) 469, 533
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 1 cut(s) 34
BstAPI GCANNNNNTGC 1 cut(s) 500
BstDEI CTNAG 2 cut(s) 74, 282
BstF5I GGATG 3 cut(s) 70, 213, 345
BstFNI CGCG 3 cut(s) 134, 170, 315
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 5 cut(s) 136, 148, 172, 317, 494
BstKTI GATC 3 cut(s) 345, 376, 421
BstMBI GATC 3 cut(s) 342, 373, 418
BstMWI GCNNNNNNNGC 4 cut(s) 123, 241, 489, 500
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 22, 444
BstSLI GKGCMC 1 cut(s) 57
BstUI CGCG 3 cut(s) 134, 170, 315
BstV1I GCAGC 2 cut(s) 29, 286
BstX2I RGATCY 2 cut(s) 373, 418
BstYI RGATCY 2 cut(s) 373, 418
BsuRI GGCC 1 cut(s) 444
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 3 cut(s) 70, 213, 345
BtsIMutI CAGTG 1 cut(s) 39
CciI TCATGA 1 cut(s) 516
CfoI GCGC 5 cut(s) 136, 148, 172, 317, 494
Cfr13I GGNCC 2 cut(s) 220, 263
Csp6I GTAC 3 cut(s) 27, 177, 253
CviAII CATG 6 cut(s) 385, 413, 451, 500, 507, 517
CviJI RGCY 6 cut(s) 20, 126, 281, 398, 444, 529
CviKI_1 RGCY 6 cut(s) 20, 126, 281, 398, 444, 529
CviQI GTAC 3 cut(s) 27, 177, 253
DdeI CTNAG 2 cut(s) 74, 282
DpnI GATC 3 cut(s) 344, 375, 420
DpnII GATC 3 cut(s) 342, 373, 418
DraIII CACNNNGTG 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 469, 533
Eco47I GGWCC 2 cut(s) 220, 263
Eco88I CYCGRG 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 2 cut(s) 469, 533
EcoT22I ATGCAT 2 cut(s) 414, 505
ErhI CCWWGG 2 cut(s) 469, 533
FaeI CATG 6 cut(s) 388, 416, 454, 503, 510, 520
FaiI YATR 8 cut(s) 13, 320, 386, 414, 452, 501, 508, 518
FaqI GGGAC 1 cut(s) 84
FatI CATG 6 cut(s) 384, 412, 450, 499, 506, 516
FauI CCCGC 1 cut(s) 320
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 18, 190, 233, 300
FokI GGATG 3 cut(s) 77, 220, 352
Fsp4HI GCNGC 4 cut(s) 18, 190, 233, 300
FspBI CTAG 1 cut(s) 470
FspI TGCGCA 1 cut(s) 493
GlaI GCGC 5 cut(s) 135, 147, 171, 316, 493
GluI GCNGC 4 cut(s) 18, 190, 233, 300
GsuI CTGGAG 2 cut(s) 390, 564
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 1 cut(s) 444
HapII CCGG 2 cut(s) 24, 261
HhaI GCGC 5 cut(s) 136, 148, 172, 317, 494
Hin1II CATG 6 cut(s) 388, 416, 454, 503, 510, 520
Hin6I GCGC 5 cut(s) 134, 146, 170, 315, 492
HinP1I GCGC 5 cut(s) 134, 146, 170, 315, 492
HinfI GANTC 2 cut(s) 119, 361
HpaII CCGG 2 cut(s) 24, 261
HphI GGTGA 3 cut(s) 106, 223, 283
Hpy166II GTNNAC 3 cut(s) 55, 223, 253
Hpy188I TCNGA 2 cut(s) 360, 366
Hpy188III TCNNGA 4 cut(s) 182, 457, 517, 543
Hpy8I GTNNAC 3 cut(s) 55, 223, 253
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 4 cut(s) 179, 194, 208, 276
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4IV ACGT 2 cut(s) 57, 225
HpyCH4V TGCA 7 cut(s) 37, 55, 322, 330, 412, 503, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 241, 489, 500
HpyF3I CTNAG 2 cut(s) 74, 282
HpySE526I ACGT 2 cut(s) 57, 225
Hsp92II CATG 6 cut(s) 388, 416, 454, 503, 510, 520
HspAI GCGC 5 cut(s) 134, 146, 170, 315, 492
KpnI GGTACC 1 cut(s) 30
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 3 cut(s) 342, 373, 418
LmnI GCTCC 2 cut(s) 25, 545
Lsp1109I GCAGC 2 cut(s) 29, 286
LweI GCATC 3 cut(s) 399, 512, 569
MaeI CTAG 1 cut(s) 470
MaeII ACGT 2 cut(s) 57, 225
MaeIII GTNAC 1 cut(s) 226
MalI GATC 3 cut(s) 344, 375, 420
MboI GATC 3 cut(s) 342, 373, 418
MboII GAAGA 4 cut(s) 90, 97, 286, 413
MflI RGATCY 2 cut(s) 373, 418
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 1 cut(s) 292
MlyI GAGTC 2 cut(s) 113, 355
MnlI CCTC 4 cut(s) 61, 84, 167, 187
Mph1103I ATGCAT 2 cut(s) 414, 505
MspI CCGG 2 cut(s) 24, 261
MspR9I CCNGG 2 cut(s) 24, 446
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 446
MvnI CGCG 3 cut(s) 134, 170, 315
MwoI GCNNNNNNNGC 4 cut(s) 123, 241, 489, 500
NciI CCSGG 1 cut(s) 24
NdeII GATC 3 cut(s) 342, 373, 418
NlaIII CATG 6 cut(s) 388, 416, 454, 503, 510, 520
NlaIV GGNNCC 1 cut(s) 28
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 226
NsbI TGCGCA 1 cut(s) 493
NsiI ATGCAT 2 cut(s) 414, 505
NspI RCATGY 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 121
PagI TCATGA 1 cut(s) 516
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 114
PctI GAATGC 1 cut(s) 464
PkrI GCNGC 4 cut(s) 19, 191, 234, 301
PleI GAGTC 2 cut(s) 113, 355
PpsI GAGTC 2 cut(s) 113, 355
Psp6I CCWGG 1 cut(s) 444
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 2 cut(s) 220, 263
PspXI VCTCGAGB 1 cut(s) 121
PsuI RGATCY 2 cut(s) 373, 418
RsaI GTAC 3 cut(s) 28, 178, 254
RsaNI GTAC 3 cut(s) 27, 177, 253
SatI GCNGC 4 cut(s) 18, 190, 233, 300
Sau3AI GATC 3 cut(s) 342, 373, 418
Sau96I GGNCC 2 cut(s) 220, 263
ScaI AGTACT 1 cut(s) 178
SchI GAGTC 2 cut(s) 113, 355
ScrFI CCNGG 2 cut(s) 24, 446
SduI GDGCHC 1 cut(s) 57
SetI ASST 8 cut(s) 22, 60, 128, 159, 219, 228, 268, 283
SfaNI GCATC 3 cut(s) 399, 512, 569
Sfr274I CTCGAG 1 cut(s) 121
SinI GGWCC 2 cut(s) 220, 263
SlaI CTCGAG 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 1 cut(s) 292
SsiI CCGC 3 cut(s) 189, 233, 313
SspMI CTAG 1 cut(s) 470
StyD4I CCNGG 2 cut(s) 22, 444
StyI CCWWGG 2 cut(s) 469, 533
TaaI ACNGT 1 cut(s) 34
TaiI ACGT 2 cut(s) 60, 228
TaqI TCGA 2 cut(s) 108, 122
TasI AATT 1 cut(s) 292
TatI WGTACW 1 cut(s) 176
TauI GCSGC 2 cut(s) 192, 235
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 17, 299
Tsp45I GTSAC 1 cut(s) 226
TspRI CASTG 1 cut(s) 39
VneI GTGCAC 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 220, 263
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 416
XhoI CTCGAG 1 cut(s) 121
XmaJI CCTAGG 1 cut(s) 469
XspI CTAG 1 cut(s) 470
ZrmI AGTACT 1 cut(s) 178
Zsp2I ATGCAT 2 cut(s) 414, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.