FvH4_6g22070

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011978
Physical Location & Seq
Forward (+)
15773806 .. 15776297
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22070.t1

Sequence Viewer

Length: 1116 bp
ATGAGGATCCGGAAAAGACAGGTCCCCTTGCCGCTCTCGTCTCTCTCTCCCGTGCCTCTATCAGATCCCAACCTCCTCAGCCTCAACCACTGCTCTTCCTCACCTCCCCTGGTGCAACCACCACAACAACAACAACCCCATATCCACCACGATCCAAAACCCTCCCAACGACTTCACGATGTGGGTCCTCTTCCTCCTCAGCCGTCTGATCATCCCAATCGCCGGCTTCTCCCGCAGATTGGGGCGTCCAGCAACGACTGCTCTGGGAGTGATTTTTGCTGGGAGCTGAAGAAGAGAAATTACTGTGCGGAGGCATTGAAAGGGGAAGACGAAGATGGATCAAGAGATGAGGGAGAGAAGAAGGGAAACGATACCAGGAAGGGAAGCATATTGGGTGCAGAAACTGTATCTGGGTTTGCTTCAGAATCGTCATGTTCTTCTCATCGAGTTCTTGGGAGATGGTTTGAGGGAGAGAAAGCGTTCCCATTCAAGAAGCGAAGAGGGAGCATCGGGGAGGCGACGAGAGTTGAGAAGGAGAAGAAATTCACGCATACTAAGATGGAGAAGAAAAGTAGTGATGGGCTGCAGCAAAGTGACGATAAGAAGCAAGACGATAGAGTAGTTACGGGAGAAGGTATCAACCAGAATGTTGTTAGTGGTACGAGTCCTAAGAAGAGAGCAAGAGGCAATGCAATTATGGAGGGATCGAGGTGCAGTCGAGTAAACGGAAGAGGATGGAGGTGTTGCCAACAAACACTCGTCGGGTACTCGCTTTGTGAGCATCACTTAGGAAAAGGAAGGCTTAGGAGCTTGAACAGCGTCCGAAGTCGGTCTCTGGCGAGTTCTATTGGCTCCGTCGAGAGCAAGTCCAAGCAATTATCAAGACAGTCGTATTTGGAGCTGCCAAAGGATGATGATTGTAAACCTGATATGCTACTTGATGTCAATCATGGCGGTGAACATCAACGACAGCAAAGTGAACACAGTGAAGAGAAAATGCCATTGGTGGTTACGAAGAAGAGAATGAAACTCGGTACTGTGAAGGCGCGATCCATGAGCAGCTTGCTTGGACAAATGAACAGTGCAACTGCAGTGGAGGATGAGAATAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

372

Amino Acids

41.16

Weight (kDa)

9.39

Isoelectric Point (pI)

59.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 234 - 274 2.8e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 34
AccII CGCG 1 cut(s) 1048
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 4 cut(s) 32, 233, 308, 954
AclWI GGATC 6 cut(s) 14, 59, 146, 346, 712, 1044
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 2 cut(s) 308, 405
AcyI GRCGYC 1 cut(s) 245
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 3 cut(s) 661, 767, 1036
AfiI CCNNNNNNNGG 2 cut(s) 222, 239
AgsI TTSAA 3 cut(s) 319, 490, 814
AjnI CCWGG 2 cut(s) 108, 374
AjuI GAANNNNNNNTTGG 2 cut(s) 986, 1018
AluBI AGCT 4 cut(s) 286, 810, 901, 1062
AluI AGCT 4 cut(s) 286, 810, 901, 1062
Alw26I GTCTC 2 cut(s) 45, 837
AlwI GGATC 6 cut(s) 14, 59, 146, 346, 712, 1044
AlwNI CAGNNNCTG 1 cut(s) 404
Aor13HI TCCGGA 1 cut(s) 9
ApeKI GCWGC 4 cut(s) 583, 586, 901, 1059
ApoI RAATTY 1 cut(s) 542
Asp700I GAANNNNTTC 2 cut(s) 479, 542
AspLEI GCGC 1 cut(s) 1048
AspS9I GGNCC 2 cut(s) 22, 185
AsuHPI GGTGA 2 cut(s) 93, 968
AvaII GGWCC 2 cut(s) 22, 185
BamHI GGATCC 1 cut(s) 6
BarI GAAGNNNNNNTAC 2 cut(s) 284, 316
BbsI GAAGAC 1 cut(s) 333
BbvCI CCTCAGC 2 cut(s) 77, 198
BbvI GCAGC 4 cut(s) 570, 598, 888, 1071
BccI CCATC 5 cut(s) 329, 453, 553, 572, 729
BceAI ACGGC 1 cut(s) 187
BciT130I CCWGG 2 cut(s) 110, 376
BclI TGATCA 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 45, 837
BfmI CTRYAG 2 cut(s) 584, 1089
BisI GCNGC 5 cut(s) 32, 584, 587, 902, 1060
BlsI GCNGC 5 cut(s) 33, 585, 588, 903, 1061
Bme1390I CCNGG 2 cut(s) 110, 376
Bme18I GGWCC 2 cut(s) 22, 185
BmgT120I GGNCC 2 cut(s) 22, 185
BmiI GGNNCC 4 cut(s) 8, 24, 186, 853
BmrFI CCNGG 2 cut(s) 110, 376
BmsI GCATC 2 cut(s) 516, 790
BpiI GAAGAC 1 cut(s) 333
Bpu10I CCTNAGC 3 cut(s) 77, 198, 803
BsaBI GATNNNNATC 1 cut(s) 945
BsaHI GRCGYC 1 cut(s) 245
BsaI GGTCTC 1 cut(s) 837
BsaJI CCNNGG 1 cut(s) 108
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 239
Bse118I RCCGGY 1 cut(s) 222
Bse3DI GCAATG 1 cut(s) 694
Bse8I GATNNNNATC 1 cut(s) 945
BseAI TCCGGA 1 cut(s) 9
BseBI CCWGG 2 cut(s) 110, 376
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 4 cut(s) 211, 740, 916, 1105
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 2 cut(s) 222, 239
BseMI GCAATG 1 cut(s) 694
BseMII CTCAG 2 cut(s) 91, 212
BseRI GAGGAG 2 cut(s) 65, 186
BseXI GCAGC 4 cut(s) 570, 598, 888, 1071
BseYI CCCAGC 1 cut(s) 279
BsgI GTGCAG 2 cut(s) 417, 733
Bsh1236I CGCG 1 cut(s) 1048
BsiSI CCGG 2 cut(s) 10, 223
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 2 cut(s) 222, 239
BsmAI GTCTC 2 cut(s) 45, 837
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 837
Bsp13I TCCGGA 1 cut(s) 9
Bsp143I GATC 7 cut(s) 6, 64, 151, 208, 338, 704, 1049
BspACI CCGC 4 cut(s) 32, 233, 308, 954
BspCNI CTCAG 2 cut(s) 90, 211
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 1 cut(s) 1048
BspLI GGNNCC 4 cut(s) 8, 24, 186, 853
BspMAI CTGCAG 2 cut(s) 588, 1093
BspPI GGATC 6 cut(s) 14, 59, 146, 346, 712, 1044
BspQI GCTCTTC 1 cut(s) 100
BspTNI GGTCTC 1 cut(s) 837
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 694
BsrFI RCCGGY 1 cut(s) 222
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 7 cut(s) 6, 64, 151, 208, 338, 704, 1049
BssNI GRCGYC 1 cut(s) 245
Bst2UI CCWGG 2 cut(s) 110, 376
Bst4CI ACNGT 6 cut(s) 305, 406, 888, 986, 1039, 1082
Bst6I CTCTTC 8 cut(s) 100, 195, 287, 493, 668, 724, 984, 1013
BstACI GRCGYC 1 cut(s) 245
BstAPI GCANNNNNTGC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 224, 1064
BstDEI CTNAG 6 cut(s) 77, 198, 555, 669, 787, 803
BstF5I GGATG 4 cut(s) 211, 740, 916, 1105
BstFNI CGCG 1 cut(s) 1048
BstHHI GCGC 1 cut(s) 1048
BstKTI GATC 7 cut(s) 9, 67, 154, 211, 341, 707, 1052
BstMAI GTCTC 2 cut(s) 45, 837
BstMBI GATC 7 cut(s) 6, 64, 151, 208, 338, 704, 1049
BstMWI GCNNNNNNNGC 4 cut(s) 232, 258, 778, 816
BstNI CCWGG 2 cut(s) 110, 376
BstSCI CCNGG 2 cut(s) 108, 374
BstSFI CTRYAG 2 cut(s) 584, 1089
BstUI CGCG 1 cut(s) 1048
BstV1I GCAGC 4 cut(s) 570, 598, 888, 1071
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 2 cut(s) 6, 64
BstYI RGATCY 2 cut(s) 6, 64
BtsCI GGATG 4 cut(s) 211, 740, 916, 1105
BtsI GCAGTG 2 cut(s) 88, 1098
BtsIMutI CAGTG 4 cut(s) 88, 991, 1087, 1098
Cac8I GCNNGC 2 cut(s) 224, 1064
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 1 cut(s) 1048
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 22, 185
CseI GACGC 2 cut(s) 234, 808
Csp6I GTAC 3 cut(s) 660, 766, 1035
CspCI CAANNNNNGTGG 2 cut(s) 1074, 1109
CviAII CATG 3 cut(s) 432, 950, 1054
CviQI GTAC 3 cut(s) 660, 766, 1035
DdeI CTNAG 6 cut(s) 77, 198, 555, 669, 787, 803
DpnI GATC 7 cut(s) 8, 66, 153, 210, 340, 706, 1051
DpnII GATC 7 cut(s) 6, 64, 151, 208, 338, 704, 1049
DraIII CACNNNGTG 1 cut(s) 181
Eam1104I CTCTTC 8 cut(s) 100, 195, 287, 493, 668, 724, 984, 1013
EarI CTCTTC 8 cut(s) 100, 195, 287, 493, 668, 724, 984, 1013
Eco31I GGTCTC 1 cut(s) 837
Eco47I GGWCC 2 cut(s) 22, 185
Eco57I CTGAAG 2 cut(s) 308, 405
EcoO109I RGGNCCY 2 cut(s) 22, 185
EcoRII CCWGG 2 cut(s) 108, 374
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 3 cut(s) 435, 953, 1057
FaiI YATR 8 cut(s) 141, 389, 433, 552, 698, 932, 951, 1055
FalI AAGNNNNNCTT 2 cut(s) 786, 818
FaqI GGGAC 1 cut(s) 8
FatI CATG 3 cut(s) 431, 949, 1053
FauI CCCGC 1 cut(s) 240
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 5 cut(s) 32, 584, 587, 902, 1060
FokI GGATG 4 cut(s) 198, 747, 923, 1112
Fsp4HI GCNGC 5 cut(s) 32, 584, 587, 902, 1060
GlaI GCGC 1 cut(s) 1047
GluI GCNGC 5 cut(s) 32, 584, 587, 902, 1060
GsaI CCCAGC 1 cut(s) 283
HapII CCGG 2 cut(s) 10, 223
HgaI GACGC 2 cut(s) 234, 808
HhaI GCGC 1 cut(s) 1048
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 3 cut(s) 435, 953, 1057
Hin6I GCGC 1 cut(s) 1046
HinP1I GCGC 1 cut(s) 1046
HinfI GANTC 2 cut(s) 425, 664
HpaII CCGG 2 cut(s) 10, 223
HphI GGTGA 2 cut(s) 93, 968
Hpy166II GTNNAC 4 cut(s) 724, 923, 959, 980
Hpy188I TCNGA 4 cut(s) 64, 208, 424, 824
Hpy188III TCNNGA 6 cut(s) 10, 176, 342, 490, 859, 882
Hpy8I GTNNAC 4 cut(s) 724, 923, 959, 980
Hpy99I CGWCG 3 cut(s) 523, 764, 860
HpyAV CCTTC 6 cut(s) 355, 373, 526, 626, 792, 1036
HpyCH4III ACNGT 6 cut(s) 305, 406, 888, 986, 1039, 1082
HpyCH4V TGCA 7 cut(s) 115, 398, 586, 692, 714, 1085, 1091
HpyF10VI GCNNNNNNNGC 4 cut(s) 232, 258, 778, 816
HpyF3I CTNAG 6 cut(s) 77, 198, 555, 669, 787, 803
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 3 cut(s) 435, 953, 1057
HspAI GCGC 1 cut(s) 1046
Kpn2I TCCGGA 1 cut(s) 9
KroI GCCGGC 1 cut(s) 222
KroNI GCCGGC 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 7 cut(s) 6, 64, 151, 208, 338, 704, 1049
LguI GCTCTTC 1 cut(s) 100
LmnI GCTCC 5 cut(s) 283, 504, 807, 857, 898
Lsp1109I GCAGC 4 cut(s) 570, 598, 888, 1071
LweI GCATC 2 cut(s) 516, 790
MaeIII GTNAC 3 cut(s) 593, 622, 1009
MalI GATC 7 cut(s) 8, 66, 153, 210, 340, 706, 1051
MbiI CCGCTC 1 cut(s) 34
MboI GATC 7 cut(s) 6, 64, 151, 208, 338, 704, 1049
MflI RGATCY 2 cut(s) 6, 64
MluCI AATT 4 cut(s) 298, 542, 693, 875
MlyI GAGTC 1 cut(s) 673
MroI TCCGGA 1 cut(s) 9
MroNI GCCGGC 1 cut(s) 222
MroXI GAANNNNTTC 2 cut(s) 479, 542
MslI CAYNNNNRTG 1 cut(s) 954
MspI CCGG 2 cut(s) 10, 223
MspR9I CCNGG 2 cut(s) 110, 376
MvaI CCWGG 2 cut(s) 110, 376
MvnI CGCG 1 cut(s) 1048
MwoI GCNNNNNNNGC 4 cut(s) 232, 258, 778, 816
NaeI GCCGGC 1 cut(s) 224
NdeII GATC 7 cut(s) 6, 64, 151, 208, 338, 704, 1049
NgoMIV GCCGGC 1 cut(s) 222
NlaIII CATG 3 cut(s) 435, 953, 1057
NlaIV GGNNCC 4 cut(s) 8, 24, 186, 853
NmuCI GTSAC 1 cut(s) 593
PciSI GCTCTTC 1 cut(s) 100
PdiI GCCGGC 1 cut(s) 224
PdmI GAANNNNTTC 2 cut(s) 479, 542
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 5 cut(s) 33, 585, 588, 903, 1061
PleI GAGTC 1 cut(s) 672
PpsI GAGTC 1 cut(s) 672
PpuMI RGGWCCY 2 cut(s) 22, 185
Psp5II RGGWCCY 2 cut(s) 22, 185
Psp6I CCWGG 2 cut(s) 108, 374
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 2 cut(s) 108, 374
PspN4I GGNNCC 4 cut(s) 8, 24, 186, 853
PspPI GGNCC 2 cut(s) 22, 185
PspPPI RGGWCCY 2 cut(s) 22, 185
PstI CTGCAG 2 cut(s) 588, 1093
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 2 cut(s) 6, 64
RsaI GTAC 3 cut(s) 661, 767, 1036
RsaNI GTAC 3 cut(s) 660, 766, 1035
RseI CAYNNNNRTG 1 cut(s) 954
SapI GCTCTTC 1 cut(s) 100
SatI GCNGC 5 cut(s) 32, 584, 587, 902, 1060
Sau3AI GATC 7 cut(s) 6, 64, 151, 208, 338, 704, 1049
Sau96I GGNCC 2 cut(s) 22, 185
SchI GAGTC 1 cut(s) 673
ScrFI CCNGG 2 cut(s) 110, 376
SfaNI GCATC 2 cut(s) 516, 790
SfcI CTRYAG 2 cut(s) 584, 1089
SinI GGWCC 2 cut(s) 22, 185
SmiMI CAYNNNNRTG 1 cut(s) 954
Sse9I AATT 4 cut(s) 298, 542, 693, 875
SsiI CCGC 4 cut(s) 32, 233, 308, 954
StyD4I CCNGG 2 cut(s) 108, 374
TaaI ACNGT 6 cut(s) 305, 406, 888, 986, 1039, 1082
TaqI TCGA 4 cut(s) 445, 707, 718, 858
TaqII GACCGA 1 cut(s) 819
TasI AATT 4 cut(s) 298, 542, 693, 875
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 1 cut(s) 425
TscAI CASTG 4 cut(s) 95, 991, 1087, 1098
TseFI GTSAC 1 cut(s) 593
TseI GCWGC 4 cut(s) 583, 586, 901, 1059
Tsp45I GTSAC 1 cut(s) 593
TspDTI ATGAA 2 cut(s) 1040, 1091
TspGWI ACGGA 2 cut(s) 741, 844
TspRI CASTG 4 cut(s) 95, 991, 1087, 1098
VpaK11BI GGWCC 2 cut(s) 22, 185
XapI RAATTY 1 cut(s) 542
XmnI GAANNNNTTC 2 cut(s) 479, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.