RLG00000023578

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011978
Physical Location & Seq
Reverse (-)
25505594 .. 25507753
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023578

Sequence Viewer

Length: 1152 bp
ATGAGGATCCGGAAAAGACAGGTCCCGTTGCCCCTCTCTTCTCTCTCGCCCGTGCCTCTACCAGATCCCCACCTCCTCAACCTCAACCACTGCTCATCATCACCTCCGCTGGTGCAACAACAACAACAACAACAACAACGTATCCACCACGATCCAAAACCCTCCCAACGACTTGATCATGTGGGCCCTCTCCCTCATCCTCAGCCGTCTGATCATCCCAATCGCCCCCGGCCGATTGGAGCAGAGACCAACGACGGCTGTGATTTTTCCGGGGAGCTGAAGAAGAGAAATTACTGCTCGGAGACATTGGAAGGGGAAGACGAAGATGGATCAAGAGATCAGGCAGAGGAGAAGGGTAATGATACCAGGAAGGGAAGCACATTGGGTGTAGAAACTGTATCTGGGTTTCCTTCAGAATCATCAAGTTCTTCTCATCGAGTTCTTGGGAGATGGTTTGAGGGAGAGAAAGCGTTCCCATTCAAGAAGCGAAGAGGGAGCTTCGGGGAGGCGGCGAGAATGGAGAAGGAGAAGAAATTAACGCAGACCAAGATGGACAAGAAATGTGATGGTCAACAGTGTGATGATAAGAAGCAAGAAGATAGAGTAATTACGAAAGAAAGCCTCGACCATACTGTCGGTAGTGGTACGAGTCCAAAGAAGAGAGCAAGAGGCAATGCAATAATGGAGGGATCGAGGTGCAGTCGAGTTAACGGAAGAGGATGGAGGTGTTGTCAACAGACACTTGTCGGGTACTCTCTTTGCGAGCATCACTTGGGCAAAGGAAGGCTTAGGAGCTTGAACAGCGTCCGAAGTCGCTCTTTTGCGAGTTCTGTCGGATCCATAGAGACCAAGTCCAAGCAATTATCAAAACCCTCGTGTTTGGAGCTGCCAAAGGAAGAAGAGCGTAAACCTGATATGCTACTAGAAGTCAATCATCATGGTGAAGATCAACGACATGAAAATGAACATGGTGAAGAGAAAAAGCCATTGGTGGTTACGAAGAAGAGAATGAAACTCGGTATTGTAAAGGCGCGATCCATGAGCAGCTTGCTTGGACAAACAAACAGTGCAACTACAGTGGATGATGAGAATAACAAATGTGGGGAACTACACCAGGAGACCCTAGTTCAGTTATTACTGTACATCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

384

Amino Acids

43.03

Weight (kDa)

9.02

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 229 - 269 2.9e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 632
AccII CGCG 1 cut(s) 1033
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 2 cut(s) 107, 509
AclWI GGATC 8 cut(s) 14, 59, 146, 337, 697, 831, 844, 1029
AcoI YGGCCR 1 cut(s) 230
AcuI CTGAAG 2 cut(s) 299, 396
AfaI GTAC 3 cut(s) 646, 752, 1142
AgsI TTSAA 2 cut(s) 481, 799
AjnI CCWGG 2 cut(s) 365, 1113
AjuI GAANNNNNNNTTGG 2 cut(s) 971, 1003
AluBI AGCT 5 cut(s) 277, 498, 795, 886, 1047
AluI AGCT 5 cut(s) 277, 498, 795, 886, 1047
Alw26I GTCTC 4 cut(s) 239, 296, 839, 1112
AlwI GGATC 8 cut(s) 14, 59, 146, 337, 697, 831, 844, 1029
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 2 cut(s) 184, 230
ApaI GGGCCC 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 886, 1044
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 1033
AspS9I GGNCC 3 cut(s) 22, 184, 185
AsuC2I CCSGG 2 cut(s) 229, 271
AsuHPI GGTGA 3 cut(s) 93, 953, 983
AvaII GGWCC 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 188
BamHI GGATCC 2 cut(s) 6, 836
BanII GRGCYC 1 cut(s) 188
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BauI CACGAG 1 cut(s) 874
BbsI GAAGAC 1 cut(s) 324
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 873, 1056
BccI CCATC 5 cut(s) 320, 444, 544, 560, 714
BceAI ACGGC 2 cut(s) 190, 271
BciT130I CCWGG 2 cut(s) 367, 1115
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 2 cut(s) 175, 211
BcnI CCSGG 2 cut(s) 229, 271
BcoDI GTCTC 4 cut(s) 239, 296, 839, 1112
BfaI CTAG 2 cut(s) 923, 1124
BfmI CTRYAG 1 cut(s) 1074
BfuI GTATCC 1 cut(s) 152
BisI GCNGC 3 cut(s) 510, 887, 1045
BlsI GCNGC 3 cut(s) 511, 888, 1046
Bme1390I CCNGG 4 cut(s) 229, 271, 367, 1115
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 3 cut(s) 22, 184, 185
BmiI GGNNCC 4 cut(s) 8, 24, 186, 838
BmrFI CCNGG 4 cut(s) 229, 271, 367, 1115
BmsI GCATC 1 cut(s) 775
BoxI GACNNNNGTC 1 cut(s) 743
BpiI GAAGAC 1 cut(s) 324
Bpu10I CCTNAGC 2 cut(s) 201, 788
BpuMI CCSGG 2 cut(s) 229, 271
BsaI GGTCTC 3 cut(s) 239, 839, 1112
BsaJI CCNNGG 2 cut(s) 227, 270
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 715, 745
Bse3DI GCAATG 1 cut(s) 679
BseAI TCCGGA 1 cut(s) 9
BseBI CCWGG 2 cut(s) 367, 1115
BseDI CCNNGG 2 cut(s) 227, 270
BseGI GGATG 4 cut(s) 196, 214, 725, 1087
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 1 cut(s) 215
BseRI GAGGAG 2 cut(s) 65, 362
BseSI GKGCMC 1 cut(s) 188
BseX3I CGGCCG 1 cut(s) 230
BseXI GCAGC 2 cut(s) 873, 1056
BsgI GTGCAG 1 cut(s) 718
Bsh1236I CGCG 1 cut(s) 1033
Bsh1285I CGRYCG 1 cut(s) 233
BshFI GGCC 2 cut(s) 186, 232
BsiEI CGRYCG 1 cut(s) 233
BsiSI CCGG 3 cut(s) 10, 229, 270
BslFI GGGAC 1 cut(s) 8
BsmAI GTCTC 4 cut(s) 239, 296, 839, 1112
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 2 cut(s) 186, 232
Bso31I GGTCTC 3 cut(s) 239, 839, 1112
Bsp120I GGGCCC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 9
Bsp1407I TGTACA 1 cut(s) 1140
BspACI CCGC 2 cut(s) 107, 509
BspANI GGCC 2 cut(s) 186, 232
BspCNI CTCAG 1 cut(s) 214
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 1 cut(s) 1033
BspLI GGNNCC 4 cut(s) 8, 24, 186, 838
BspPI GGATC 8 cut(s) 14, 59, 146, 337, 697, 831, 844, 1029
BspQI GCTCTTC 1 cut(s) 894
BspTNI GGTCTC 3 cut(s) 239, 839, 1112
BsrDI GCAATG 1 cut(s) 679
BsrGI TGTACA 1 cut(s) 1140
BssECI CCNNGG 2 cut(s) 227, 270
BssSI CACGAG 1 cut(s) 874
Bst2BI CACGAG 1 cut(s) 874
Bst2UI CCWGG 2 cut(s) 367, 1115
Bst4CI ACNGT 6 cut(s) 397, 576, 634, 1067, 1078, 1140
Bst6I CTCTTC 8 cut(s) 43, 278, 484, 653, 709, 894, 969, 998
BstAUI TGTACA 1 cut(s) 1140
BstC8I GCNNGC 2 cut(s) 764, 1049
BstDEI CTNAG 2 cut(s) 201, 788
BstF5I GGATG 4 cut(s) 196, 214, 725, 1087
BstFNI CGCG 1 cut(s) 1033
BstHHI GCGC 1 cut(s) 1033
BstMAI GTCTC 4 cut(s) 239, 296, 839, 1112
BstMCI CGRYCG 1 cut(s) 233
BstMWI GCNNNNNNNGC 1 cut(s) 801
BstNI CCWGG 2 cut(s) 367, 1115
BstPAI GACNNNNGTC 1 cut(s) 743
BstSCI CCNGG 4 cut(s) 227, 269, 365, 1113
BstSFI CTRYAG 1 cut(s) 1074
BstSLI GKGCMC 1 cut(s) 188
BstUI CGCG 1 cut(s) 1033
BstV1I GCAGC 2 cut(s) 873, 1056
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 3 cut(s) 6, 64, 836
BstYI RGATCY 3 cut(s) 6, 64, 836
BstZI CGGCCG 1 cut(s) 230
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 2 cut(s) 186, 232
BtsCI GGATG 4 cut(s) 196, 214, 725, 1087
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 4 cut(s) 88, 581, 1072, 1083
Cac8I GCNNGC 2 cut(s) 764, 1049
CfoI GCGC 1 cut(s) 1033
Cfr13I GGNCC 3 cut(s) 22, 184, 185
CseI GACGC 1 cut(s) 793
Csp6I GTAC 3 cut(s) 645, 751, 1141
CspCI CAANNNNNGTGG 2 cut(s) 1059, 1094
CviAII CATG 5 cut(s) 179, 938, 956, 968, 1039
CviQI GTAC 3 cut(s) 645, 751, 1141
DdeI CTNAG 2 cut(s) 201, 788
DrdI GACNNNNNNGTC 1 cut(s) 632
DseDI GACNNNNNNGTC 1 cut(s) 632
EaeI YGGCCR 1 cut(s) 230
EagI CGGCCG 1 cut(s) 230
Eam1104I CTCTTC 8 cut(s) 43, 278, 484, 653, 709, 894, 969, 998
EarI CTCTTC 8 cut(s) 43, 278, 484, 653, 709, 894, 969, 998
EclXI CGGCCG 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 3 cut(s) 239, 839, 1112
Eco47I GGWCC 1 cut(s) 22
Eco52I CGGCCG 1 cut(s) 230
Eco57I CTGAAG 2 cut(s) 299, 396
EcoO109I RGGNCCY 2 cut(s) 22, 185
EcoRII CCWGG 2 cut(s) 365, 1113
EcoT38I GRGCYC 1 cut(s) 188
FaeI CATG 5 cut(s) 182, 941, 959, 971, 1042
FaiI YATR 8 cut(s) 180, 630, 842, 917, 939, 957, 969, 1040
FalI AAGNNNNNCTT 4 cut(s) 771, 803, 802, 834
FaqI GGGAC 1 cut(s) 8
FatI CATG 5 cut(s) 178, 937, 955, 967, 1038
FbaI TGATCA 2 cut(s) 175, 211
Fnu4HI GCNGC 3 cut(s) 510, 887, 1045
FokI GGATG 4 cut(s) 183, 201, 732, 1094
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 3 cut(s) 510, 887, 1045
FspBI CTAG 2 cut(s) 923, 1124
GlaI GCGC 1 cut(s) 1032
GluI GCNGC 3 cut(s) 510, 887, 1045
HaeIII GGCC 2 cut(s) 186, 232
HapII CCGG 3 cut(s) 10, 229, 270
HgaI GACGC 1 cut(s) 793
HhaI GCGC 1 cut(s) 1033
Hin1II CATG 5 cut(s) 182, 941, 959, 971, 1042
Hin6I GCGC 1 cut(s) 1031
HinP1I GCGC 1 cut(s) 1031
HincII GTYRAC 3 cut(s) 572, 709, 734
HindII GTYRAC 3 cut(s) 572, 709, 734
HinfI GANTC 2 cut(s) 416, 649
HpaI GTTAAC 1 cut(s) 709
HpaII CCGG 3 cut(s) 10, 229, 270
HphI GGTGA 3 cut(s) 93, 953, 983
Hpy166II GTNNAC 4 cut(s) 572, 709, 734, 908
Hpy188I TCNGA 5 cut(s) 211, 301, 415, 809, 836
Hpy188III TCNNGA 3 cut(s) 10, 333, 481
Hpy8I GTNNAC 4 cut(s) 572, 709, 734, 908
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 6 cut(s) 305, 346, 364, 420, 517, 777
HpyCH4III ACNGT 6 cut(s) 397, 576, 634, 1067, 1078, 1140
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 4 cut(s) 115, 677, 699, 1070
HpyF10VI GCNNNNNNNGC 1 cut(s) 801
HpyF3I CTNAG 2 cut(s) 201, 788
HpySE526I ACGT 1 cut(s) 139
Hsp92II CATG 5 cut(s) 182, 941, 959, 971, 1042
HspAI GCGC 1 cut(s) 1031
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 2 cut(s) 175, 211
KspAI GTTAAC 1 cut(s) 709
LguI GCTCTTC 1 cut(s) 894
LmnI GCTCC 5 cut(s) 239, 274, 495, 792, 883
Lsp1109I GCAGC 2 cut(s) 873, 1056
LweI GCATC 1 cut(s) 775
MaeI CTAG 2 cut(s) 923, 1124
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 1 cut(s) 994
MflI RGATCY 3 cut(s) 6, 64, 836
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 4 cut(s) 289, 533, 606, 860
MlyI GAGTC 1 cut(s) 658
MmeI TCCRAC 1 cut(s) 814
MroI TCCGGA 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 2 cut(s) 536, 708
MslI CAYNNNNRTG 2 cut(s) 939, 960
MspA1I CMGCKG 1 cut(s) 109
MspI CCGG 3 cut(s) 10, 229, 270
MspR9I CCNGG 4 cut(s) 229, 271, 367, 1115
MvaI CCWGG 2 cut(s) 367, 1115
MvnI CGCG 1 cut(s) 1033
MwoI GCNNNNNNNGC 1 cut(s) 801
NciI CCSGG 2 cut(s) 229, 271
NlaIII CATG 5 cut(s) 182, 941, 959, 971, 1042
NlaIV GGNNCC 4 cut(s) 8, 24, 186, 838
PciSI GCTCTTC 1 cut(s) 894
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 1 cut(s) 416
PflFI GACNNNGTC 1 cut(s) 850
PkrI GCNGC 3 cut(s) 511, 888, 1046
PleI GAGTC 1 cut(s) 657
PpsI GAGTC 1 cut(s) 657
PpuMI RGGWCCY 1 cut(s) 22
PshAI GACNNNNGTC 1 cut(s) 743
Psp5II RGGWCCY 1 cut(s) 22
Psp6I CCWGG 2 cut(s) 365, 1113
PspGI CCWGG 2 cut(s) 365, 1113
PspN4I GGNNCC 4 cut(s) 8, 24, 186, 838
PspOMI GGGCCC 1 cut(s) 184
PspPI GGNCC 3 cut(s) 22, 184, 185
PspPPI RGGWCCY 1 cut(s) 22
PsuI RGATCY 3 cut(s) 6, 64, 836
PsyI GACNNNGTC 1 cut(s) 850
RsaI GTAC 3 cut(s) 646, 752, 1142
RsaNI GTAC 3 cut(s) 645, 751, 1141
RseI CAYNNNNRTG 2 cut(s) 939, 960
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 2 cut(s) 536, 708
SatI GCNGC 3 cut(s) 510, 887, 1045
Sau96I GGNCC 3 cut(s) 22, 184, 185
SchI GAGTC 1 cut(s) 658
ScrFI CCNGG 4 cut(s) 229, 271, 367, 1115
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 1 cut(s) 775
SfcI CTRYAG 1 cut(s) 1074
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 2 cut(s) 939, 960
Sse9I AATT 4 cut(s) 289, 533, 606, 860
SsiI CCGC 2 cut(s) 107, 509
SspMI CTAG 2 cut(s) 923, 1124
StyD4I CCNGG 4 cut(s) 227, 269, 365, 1113
TaaI ACNGT 6 cut(s) 397, 576, 634, 1067, 1078, 1140
TaiI ACGT 1 cut(s) 142
TaqI TCGA 4 cut(s) 436, 624, 692, 703
TasI AATT 4 cut(s) 289, 533, 606, 860
TatI WGTACW 1 cut(s) 1140
TauI GCSGC 1 cut(s) 512
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 2 cut(s) 536, 708
Tru9I TTAA 2 cut(s) 536, 708
TscAI CASTG 4 cut(s) 95, 581, 1072, 1083
TseI GCWGC 2 cut(s) 886, 1044
TspDTI ATGAA 3 cut(s) 972, 978, 1025
TspGWI ACGGA 1 cut(s) 726
TspRI CASTG 4 cut(s) 95, 581, 1072, 1083
Tth111I GACNNNGTC 1 cut(s) 850
VpaK11BI GGWCC 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 2 cut(s) 923, 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.