Rh3BG259000

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011978
Physical Location & Seq
Forward (+)
25090405 .. 25090989
585 bp
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UTR
Exon/CDS
Intron
Rh3BG259000.1

Sequence Viewer

Length: 585 bp
ATGGAGAAGGAGAAGAAATTAACGCAGACCAAGAAGGACAAGAAATGTGATGGTCAACAGTTTGATGATAAGAAGCAAGAAGATAGAGTAATTACGAAAGAAATCCTCGACCATACTGTTGGTAGTGGTACGAGTCCAAAGAAGAGAGCAAGAGGCAATGCAATAATGGAGGGATCGAGGTGCAGTCGAGTTAACGGAAGAGGATGGAGGTGTTGTCAACAGACACTTGTCGGGTACTCTCTTTGCGAGCATCACTTGGGCAAAGGAAGGCTTAGGAGCTTGAACAGCGTCCGAAGTCGCTCTTTTGCGAGTTCTGTTGGATCCATAGAGACCAAGTCCAAGCAATTATCAAAACCCTCGTGTTTGGAGCTGCCAAAGGAAGAAGAGCGTAAACCTGATATGCTACTAGATGTCAATCATCATGGTGAAGATCAACGACACGAAAATGAATACGGTGAAGAGAAAAAGCCGTTGGTGGTTACGAAGAAGAGAATGAAACTCGGTATTGTAAAGGCACGATCCATGAGCAGCTTGCTTGGGCAAACGAACAGTGCAACTGCAGTGGATGATGAGAATAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

194

Amino Acids

21.9

Weight (kDa)

9.49

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 57 - 97 1e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 181, 315, 328, 513
AfaI GTAC 2 cut(s) 130, 236
AgsI TTSAA 1 cut(s) 283
AjuI GAANNNNNNNTTGG 2 cut(s) 455, 487
AluBI AGCT 3 cut(s) 279, 370, 531
AluI AGCT 3 cut(s) 279, 370, 531
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 4 cut(s) 181, 315, 328, 513
ApeKI GCWGC 2 cut(s) 370, 528
AsuHPI GGTGA 2 cut(s) 437, 467
BamHI GGATCC 1 cut(s) 320
BauI CACGAG 1 cut(s) 358
BbvI GCAGC 2 cut(s) 357, 540
BccI CCATC 2 cut(s) 44, 198
BceAI ACGGC 1 cut(s) 454
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 1 cut(s) 558
BisI GCNGC 2 cut(s) 371, 529
BlsI GCNGC 2 cut(s) 372, 530
BmiI GGNNCC 1 cut(s) 322
BmsI GCATC 1 cut(s) 259
BoxI GACNNNNGTC 1 cut(s) 227
Bpu10I CCTNAGC 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 323
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bse3DI GCAATG 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 414
BseGI GGATG 2 cut(s) 209, 571
BseJI GATNNNNATC 1 cut(s) 414
BseMI GCAATG 1 cut(s) 163
BseXI GCAGC 2 cut(s) 357, 540
BsgI GTGCAG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 323
Bso31I GGTCTC 1 cut(s) 323
Bsp143I GATC 4 cut(s) 173, 320, 430, 518
BspLI GGNNCC 1 cut(s) 322
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 4 cut(s) 181, 315, 328, 513
BspQI GCTCTTC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 163
BssMI GATC 4 cut(s) 173, 320, 430, 518
BssSI CACGAG 1 cut(s) 358
Bst2BI CACGAG 1 cut(s) 358
Bst4CI ACNGT 4 cut(s) 60, 118, 455, 551
Bst6I CTCTTC 5 cut(s) 137, 193, 378, 453, 482
BstC8I GCNNGC 2 cut(s) 248, 533
BstDEI CTNAG 1 cut(s) 272
BstF5I GGATG 2 cut(s) 209, 571
BstKTI GATC 4 cut(s) 176, 323, 433, 521
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 4 cut(s) 173, 320, 430, 518
BstMWI GCNNNNNNNGC 1 cut(s) 285
BstPAI GACNNNNGTC 1 cut(s) 227
BstSFI CTRYAG 1 cut(s) 558
BstV1I GCAGC 2 cut(s) 357, 540
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 119
BstYI RGATCY 1 cut(s) 320
BtsCI GGATG 2 cut(s) 209, 571
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 2 cut(s) 556, 567
Cac8I GCNNGC 2 cut(s) 248, 533
CseI GACGC 1 cut(s) 277
Csp6I GTAC 2 cut(s) 129, 235
CspCI CAANNNNNGTGG 2 cut(s) 543, 578
CviAII CATG 2 cut(s) 422, 523
CviJI RGCY 5 cut(s) 271, 279, 370, 469, 531
CviKI_1 RGCY 5 cut(s) 271, 279, 370, 469, 531
CviQI GTAC 2 cut(s) 129, 235
DdeI CTNAG 1 cut(s) 272
DpnI GATC 4 cut(s) 175, 322, 432, 520
DpnII GATC 4 cut(s) 173, 320, 430, 518
Eam1104I CTCTTC 5 cut(s) 137, 193, 378, 453, 482
EarI CTCTTC 5 cut(s) 137, 193, 378, 453, 482
Eco31I GGTCTC 1 cut(s) 323
FaeI CATG 2 cut(s) 425, 526
FaiI YATR 5 cut(s) 114, 326, 401, 423, 524
FalI AAGNNNNNCTT 4 cut(s) 255, 287, 286, 318
FatI CATG 2 cut(s) 421, 522
Fnu4HI GCNGC 2 cut(s) 371, 529
FokI GGATG 2 cut(s) 216, 578
Fsp4HI GCNGC 2 cut(s) 371, 529
FspBI CTAG 1 cut(s) 407
GluI GCNGC 2 cut(s) 371, 529
HgaI GACGC 1 cut(s) 277
Hin1II CATG 2 cut(s) 425, 526
HincII GTYRAC 3 cut(s) 56, 193, 218
HindII GTYRAC 3 cut(s) 56, 193, 218
HinfI GANTC 1 cut(s) 133
HpaI GTTAAC 1 cut(s) 193
HphI GGTGA 2 cut(s) 437, 467
Hpy166II GTNNAC 4 cut(s) 56, 193, 218, 392
Hpy188I TCNGA 1 cut(s) 293
Hpy8I GTNNAC 4 cut(s) 56, 193, 218, 392
HpyAV CCTTC 2 cut(s) 28, 261
HpyCH4III ACNGT 4 cut(s) 60, 118, 455, 551
HpyCH4V TGCA 4 cut(s) 161, 183, 554, 560
HpyF10VI GCNNNNNNNGC 1 cut(s) 285
HpyF3I CTNAG 1 cut(s) 272
Hsp92II CATG 2 cut(s) 425, 526
KspAI GTTAAC 1 cut(s) 193
Kzo9I GATC 4 cut(s) 173, 320, 430, 518
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 2 cut(s) 276, 367
LpnPI CCDG 1 cut(s) 408
Lsp1109I GCAGC 2 cut(s) 357, 540
LweI GCATC 1 cut(s) 259
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 478
MalI GATC 4 cut(s) 175, 322, 432, 520
MboI GATC 4 cut(s) 173, 320, 430, 518
MflI RGATCY 1 cut(s) 320
MluCI AATT 3 cut(s) 17, 90, 344
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 7 cut(s) 116, 146, 163, 171, 194, 201, 367
MseI TTAA 2 cut(s) 20, 192
MslI CAYNNNNRTG 2 cut(s) 423, 444
MwoI GCNNNNNNNGC 1 cut(s) 285
NdeII GATC 4 cut(s) 173, 320, 430, 518
NlaIII CATG 2 cut(s) 425, 526
NlaIV GGNNCC 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 378
PflFI GACNNNGTC 1 cut(s) 334
PkrI GCNGC 2 cut(s) 372, 530
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
PshAI GACNNNNGTC 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 322
PstI CTGCAG 1 cut(s) 562
PsuI RGATCY 1 cut(s) 320
PsyI GACNNNGTC 1 cut(s) 334
RsaI GTAC 2 cut(s) 130, 236
RsaNI GTAC 2 cut(s) 129, 235
RseI CAYNNNNRTG 2 cut(s) 423, 444
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 2 cut(s) 20, 192
SatI GCNGC 2 cut(s) 371, 529
Sau3AI GATC 4 cut(s) 173, 320, 430, 518
SchI GAGTC 1 cut(s) 142
SetI ASST 6 cut(s) 182, 212, 281, 372, 397, 533
SfaNI GCATC 1 cut(s) 259
SfcI CTRYAG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 423, 444
Sse9I AATT 3 cut(s) 17, 90, 344
SspMI CTAG 1 cut(s) 407
TaaI ACNGT 4 cut(s) 60, 118, 455, 551
TaqI TCGA 3 cut(s) 108, 176, 187
TasI AATT 3 cut(s) 17, 90, 344
Tru1I TTAA 2 cut(s) 20, 192
Tru9I TTAA 2 cut(s) 20, 192
TscAI CASTG 2 cut(s) 556, 567
TseI GCWGC 2 cut(s) 370, 528
TspDTI ATGAA 2 cut(s) 462, 509
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 2 cut(s) 556, 567
Tth111I GACNNNGTC 1 cut(s) 334
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.