MD12G1056100.v1.1

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011978
Physical Location & Seq
Forward (+)
6379333 .. 6381783
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1056100.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGAGGATCCGGAAACGACAGGTGCCCTTCCCTCTGTCGTCTCTTTCTCCGGTGCCTTTATCAGATCCCCTCCTCCACGACCTCAACTACTCACCACCACCCGTGGTGCAACTCAACCACCGCAATGATCCTCCAAATCCCTCCAACCCAGTACAGAATACTGCCCATGTGGGCCATCCCCATTCTCAGCCGTCCGATCAACGCGTCTCGCCGATCGGACGAGCAGGCAAAGGTTGGGGTTTTGTTGATGCTGCTGGAGATAATAAGCACAAGGAGAACAAGCAAGATTGTGGTTTGGAGCCATTGATATTGAAAGGGGAAGAAGATGAGAGAGAATCAAGAGAGGGAGGAGATCAGAAGGGAAATGAGGATGATGAAATCAGGGAGGAAAGCATATTGGTTGCAGAAACATTAATCGGGTTTGCTTCGGGGTCGTCTAGTTCTTCTCACCCAGCTGCTGGGAGTTGTTTTCAAGGAGAGAGAGCGTTTCCATTGAAGAAGCGAAGAGGGAGCTTCGAGAGAGGAGCGAATAATATCAGACAGACGATAATGGATGATCAGAAAGACAAGAAATCAATGAAAACGAAGACGAACAAGAAAAATGTACCTCGACAGCAACAAGGTGAAGATGATCGTCAGAAGCAAGACGATCATGTCAGTGATGATAATAGAGTCAATAGTGGTGTTAGTGCAACAAGAAAGAGGGCAAGAGGAGGTGCACTCATGGAGGGGTCGCGGTGCAGTCGCGTTAATGGAAGAGGATGGAGGTGTTTCCAACAAACACTTGTTGGGTACTCTCTTTGCGAGCATCACTTGGGCAAGGGTAGGTTGAGGAGCATGAATAGTGTTAGAAGCCGGTCGACGGCAGTGGCAACTAGTACTACTACCACTTCTACTATTACTAGAGCTGCTTCCACTGAGACTGAGTTTAAGCAATTATCAGTATCTAGAATATTATCCTTGGAGTCACCGCCGCTGAAAGAAGACACCAAAGCTGTTCTGCTGCTGGATGATCGTGATATCGGTGAAGGTAAAGGAAATGAAGGTGAAGAGGAAGAGAAGAAGCCATTGCTGGTTACAAAGAAGCGGATGAAGGTTGGGATTGTTAAAGCCCGATCCATGAGCAGCTTGCTAGGTCAAACAAATAGTAGTGCAATTTCAGTAGTGGCTGATGAGGATGAGGATAATAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

399

Amino Acids

43.79

Weight (kDa)

9.06

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 243 - 283 1e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 653
AccB1I GGYRCC 2 cut(s) 22, 52
AccB7I CCANNNNNTGG 1 cut(s) 458
AccI GTMKAC 1 cut(s) 860
AccII CGCG 3 cut(s) 204, 736, 747
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 5 cut(s) 121, 736, 971, 974, 1087
AclWI GGATC 4 cut(s) 14, 59, 122, 1110
AfaI GTAC 4 cut(s) 153, 606, 794, 880
AfiI CCNNNNNNNGG 2 cut(s) 458, 862
AflIII ACRYGT 1 cut(s) 202
AgsI TTSAA 3 cut(s) 313, 473, 496
AhlI ACTAGT 1 cut(s) 875
AluBI AGCT 5 cut(s) 455, 513, 908, 995, 1128
AluI AGCT 5 cut(s) 455, 513, 908, 995, 1128
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 3 cut(s) 45, 211, 914
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 4 cut(s) 14, 59, 122, 1110
AlwNI CAGNNNCTG 1 cut(s) 458
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 1 cut(s) 172
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 5 cut(s) 251, 455, 908, 1003, 1125
AseI ATTAAT 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 6 cut(s) 84, 440, 635, 960, 1037, 1058
BaeGI GKGCMC 2 cut(s) 27, 721
BamHI GGATCC 1 cut(s) 6
BanI GGYRCC 2 cut(s) 22, 52
BbsI GAAGAC 2 cut(s) 593, 990
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 5 cut(s) 238, 442, 895, 990, 1137
BccI CCATC 2 cut(s) 183, 756
BceAI ACGGC 2 cut(s) 175, 879
BclI TGATCA 1 cut(s) 556
BcoDI GTCTC 3 cut(s) 45, 211, 914
BcuI ACTAGT 1 cut(s) 875
BfaI CTAG 5 cut(s) 438, 876, 903, 948, 1133
BisI GCNGC 6 cut(s) 252, 456, 909, 974, 1004, 1126
BlsI GCNGC 6 cut(s) 253, 457, 910, 975, 1005, 1127
BmcAI AGTACT 1 cut(s) 880
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 4 cut(s) 8, 24, 54, 300
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 238, 817
BmuI ACTGGG 1 cut(s) 143
BpiI GAAGAC 2 cut(s) 593, 990
BplI GAGNNNNNCTC 2 cut(s) 705, 737
BpmI CTGGAG 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 102, 960
BsaWI WCCGGW 2 cut(s) 9, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 862
Bse118I RCCGGY 1 cut(s) 855
Bse1I ACTGG 1 cut(s) 149
Bse3DI GCAATG 2 cut(s) 130, 1067
BseAI TCCGGA 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 102, 960
BseGI GGATG 7 cut(s) 175, 376, 559, 767, 1015, 1095, 1183
BseLI CCNNNNNNNGG 2 cut(s) 458, 862
BseMI GCAATG 2 cut(s) 130, 1067
BseMII CTCAG 3 cut(s) 200, 909, 915
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 5 cut(s) 62, 363, 537, 726, 847
BseSI GKGCMC 2 cut(s) 27, 721
BseXI GCAGC 5 cut(s) 238, 442, 895, 990, 1137
BseYI CCCAGC 2 cut(s) 451, 458
BsgI GTGCAG 1 cut(s) 760
Bsh1236I CGCG 3 cut(s) 204, 736, 747
Bsh1285I CGRYCG 2 cut(s) 216, 860
BshFI GGCC 1 cut(s) 174
BshNI GGYRCC 2 cut(s) 22, 52
BsiEI CGRYCG 2 cut(s) 216, 860
BsiHKAI GWGCWC 1 cut(s) 721
BsiSI CCGG 3 cut(s) 10, 50, 856
BslI CCNNNNNNNGG 2 cut(s) 458, 862
BsmAI GTCTC 3 cut(s) 45, 211, 914
BsmBI CGTCTC 2 cut(s) 45, 211
BsnI GGCC 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 27, 721
Bsp13I TCCGGA 1 cut(s) 9
BspACI CCGC 5 cut(s) 121, 736, 971, 974, 1087
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 3 cut(s) 199, 910, 916
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 3 cut(s) 204, 736, 747
BspLI GGNNCC 4 cut(s) 8, 24, 54, 300
BspPI GGATC 4 cut(s) 14, 59, 122, 1110
BspT107I GGYRCC 2 cut(s) 22, 52
BsrDI GCAATG 2 cut(s) 130, 1067
BsrFI RCCGGY 1 cut(s) 855
BsrI ACTGG 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 855
BssECI CCNNGG 2 cut(s) 102, 960
BssT1I CCWWGG 1 cut(s) 960
Bst6I CTCTTC 4 cut(s) 499, 751, 1044, 1050
BstC8I GCNNGC 3 cut(s) 226, 806, 1130
BstDEI CTNAG 3 cut(s) 186, 918, 924
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 7 cut(s) 175, 376, 559, 767, 1015, 1095, 1183
BstFNI CGCG 3 cut(s) 204, 736, 747
BstMAI GTCTC 3 cut(s) 45, 211, 914
BstMCI CGRYCG 2 cut(s) 216, 860
BstSLI GKGCMC 2 cut(s) 27, 721
BstUI CGCG 3 cut(s) 204, 736, 747
BstV1I GCAGC 5 cut(s) 238, 442, 895, 990, 1137
BstV2I GAAGAC 2 cut(s) 593, 990
BstX2I RGATCY 2 cut(s) 6, 64
BstYI RGATCY 2 cut(s) 6, 64
BsuRI GGCC 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 7 cut(s) 175, 376, 559, 767, 1015, 1095, 1183
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 3 cut(s) 664, 873, 915
Cac8I GCNNGC 3 cut(s) 226, 806, 1130
CaiI CAGNNNCTG 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 855
Cfr13I GGNCC 1 cut(s) 172
CseI GACGC 1 cut(s) 193
Csp6I GTAC 4 cut(s) 152, 605, 793, 879
CviAII CATG 5 cut(s) 167, 653, 724, 838, 1120
CviQI GTAC 4 cut(s) 152, 605, 793, 879
DdeI CTNAG 3 cut(s) 186, 918, 924
DrdI GACNNNNNNGTC 1 cut(s) 653
DseDI GACNNNNNNGTC 1 cut(s) 653
Eam1104I CTCTTC 4 cut(s) 499, 751, 1044, 1050
EarI CTCTTC 4 cut(s) 499, 751, 1044, 1050
Eco130I CCWWGG 1 cut(s) 960
Eco32I GATATC 1 cut(s) 1021
EcoRV GATATC 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 960
ErhI CCWWGG 1 cut(s) 960
Esp3I CGTCTC 2 cut(s) 45, 211
FaeI CATG 5 cut(s) 170, 656, 727, 841, 1123
FaiI YATR 6 cut(s) 168, 395, 654, 725, 839, 1121
FatI CATG 5 cut(s) 166, 652, 723, 837, 1119
FbaI TGATCA 1 cut(s) 556
FblI GTMKAC 1 cut(s) 860
Fnu4HI GCNGC 6 cut(s) 252, 456, 909, 974, 1004, 1126
FokI GGATG 7 cut(s) 162, 383, 566, 774, 1022, 1102, 1190
Fsp4HI GCNGC 6 cut(s) 252, 456, 909, 974, 1004, 1126
FspBI CTAG 5 cut(s) 438, 876, 903, 948, 1133
GluI GCNGC 6 cut(s) 252, 456, 909, 974, 1004, 1126
GsaI CCCAGC 2 cut(s) 455, 462
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 1 cut(s) 174
HapII CCGG 3 cut(s) 10, 50, 856
HgaI GACGC 1 cut(s) 193
Hin1II CATG 5 cut(s) 170, 656, 727, 841, 1123
HincII GTYRAC 1 cut(s) 861
HindII GTYRAC 1 cut(s) 861
HinfI GANTC 3 cut(s) 335, 672, 965
HpaII CCGG 3 cut(s) 10, 50, 856
HphI GGTGA 6 cut(s) 84, 440, 635, 960, 1037, 1058
Hpy166II GTNNAC 2 cut(s) 719, 861
Hpy188I TCNGA 7 cut(s) 64, 196, 218, 357, 539, 561, 639
Hpy188III TCNNGA 5 cut(s) 10, 339, 517, 948, 1016
Hpy8I GTNNAC 2 cut(s) 719, 861
Hpy99I CGWCG 1 cut(s) 865
HpyAV CCTTC 5 cut(s) 37, 352, 1022, 1037, 1087
HpyCH4V TGCA 6 cut(s) 109, 404, 692, 719, 741, 1154
HpyF3I CTNAG 3 cut(s) 186, 918, 924
Hsp92II CATG 5 cut(s) 170, 656, 727, 841, 1123
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 556
LmnI GCTCC 4 cut(s) 298, 510, 524, 834
Lsp1109I GCAGC 5 cut(s) 238, 442, 895, 990, 1137
LweI GCATC 2 cut(s) 238, 817
MaeI CTAG 5 cut(s) 438, 876, 903, 948, 1133
MaeIII GTNAC 2 cut(s) 966, 1075
MflI RGATCY 2 cut(s) 6, 64
MhlI GDGCHC 2 cut(s) 27, 721
MluCI AATT 2 cut(s) 935, 1155
MluI ACGCGT 1 cut(s) 202
MlyI GAGTC 2 cut(s) 681, 974
MmeI TCCRAC 2 cut(s) 168, 799
MroI TCCGGA 1 cut(s) 9
MseI TTAA 4 cut(s) 413, 750, 930, 1107
MslI CAYNNNNRTG 2 cut(s) 123, 657
MspA1I CMGCKG 2 cut(s) 455, 976
MspI CCGG 3 cut(s) 10, 50, 856
MvnI CGCG 3 cut(s) 204, 736, 747
NlaIII CATG 5 cut(s) 170, 656, 727, 841, 1123
NlaIV GGNNCC 4 cut(s) 8, 24, 54, 300
NmuCI GTSAC 1 cut(s) 966
PfeI GAWTC 1 cut(s) 335
PflMI CCANNNNNTGG 1 cut(s) 458
PkrI GCNGC 6 cut(s) 253, 457, 910, 975, 1005, 1127
Ple19I CGATCG 1 cut(s) 216
PleI GAGTC 2 cut(s) 680, 973
PpsI GAGTC 2 cut(s) 680, 973
PshBI ATTAAT 1 cut(s) 413
PspFI CCCAGC 2 cut(s) 451, 458
PspN4I GGNNCC 4 cut(s) 8, 24, 54, 300
PspPI GGNCC 1 cut(s) 172
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 2 cut(s) 6, 64
PvuI CGATCG 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 4 cut(s) 153, 606, 794, 880
RsaNI GTAC 4 cut(s) 152, 605, 793, 879
RseI CAYNNNNRTG 2 cut(s) 123, 657
SalI GTCGAC 1 cut(s) 859
SaqAI TTAA 4 cut(s) 413, 750, 930, 1107
SatI GCNGC 6 cut(s) 252, 456, 909, 974, 1004, 1126
Sau96I GGNCC 1 cut(s) 172
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 2 cut(s) 681, 974
SduI GDGCHC 2 cut(s) 27, 721
SfaNI GCATC 2 cut(s) 238, 817
SmiMI CAYNNNNRTG 2 cut(s) 123, 657
SpeI ACTAGT 1 cut(s) 875
Sse9I AATT 2 cut(s) 935, 1155
SsiI CCGC 5 cut(s) 121, 736, 971, 974, 1087
SspI AATATT 1 cut(s) 954
SspMI CTAG 5 cut(s) 438, 876, 903, 948, 1133
StyI CCWWGG 1 cut(s) 960
TaqI TCGA 3 cut(s) 516, 610, 860
TasI AATT 2 cut(s) 935, 1155
TatI WGTACW 2 cut(s) 151, 878
TauI GCSGC 1 cut(s) 976
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 4 cut(s) 413, 750, 930, 1107
Tru9I TTAA 4 cut(s) 413, 750, 930, 1107
TscAI CASTG 3 cut(s) 664, 873, 922
TseFI GTSAC 1 cut(s) 966
TseI GCWGC 5 cut(s) 251, 455, 908, 1003, 1125
Tsp45I GTSAC 1 cut(s) 966
TspDTI ATGAA 5 cut(s) 390, 593, 854, 1056, 1106
TspRI CASTG 3 cut(s) 664, 873, 922
Van91I CCANNNNNTGG 1 cut(s) 458
VneI GTGCAC 1 cut(s) 717
VspI ATTAAT 1 cut(s) 413
XbaI TCTAGA 1 cut(s) 947
XmiI GTMKAC 1 cut(s) 860
XspI CTAG 5 cut(s) 438, 876, 903, 948, 1133
ZrmI AGTACT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.