pycom12g04900

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011978
Physical Location & Seq
Forward (+)
4857945 .. 4860055
2111 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04900.1

Sequence Viewer

Length: 1197 bp
ATGAGGATCCGGAAACGACAGGTGCCCTTCCCTCTGTCGTCTCTTTCTCCGGTGCCTTTATCAGATCCCCTCCTCCACGACCTCAACTACTCACCACCACCCGTGGTGCAACTCAACCACCGCCATGATCCTCCAAATCCCTCCAACCCAGTAGAGAAAACTACCCATGTGGGCCATCCCCATCCTCAGCCGTCCGATCAACACGTCTCGCCGATCGGACGAGCAGGCAAAGGTTGGGGTTTTGTTGATGCTGCTGGAGATAATAAGCACAAGGAGAACAAGCAAGCTTGTGGTTTGGAGCGCCCATTGATATTGAAAGGGGAAGAAGATGAGAGAGAATCAAGAGAGGGAGGAGATCAGAAGGGAAATGAGGATGATGAAATCAGGGAGGAAAGCATATTGGTTGCAGAAACATTAATGGGGTTTGCTTCGGGGTCGTCTAGTTCTTCTCACCCAGCTTCTGGGAGTTGGTTTCAAGGAGAGAGAGCGTTTCCATTGAAGAAGCGAAGAGGGAGCTTCGAGAGTGGAGCGAATAATATCAGACAGACGATAATGGATGATCAGAAAGACAAGAAATCAATGAAAACGAAGACGAACAAGAAAAGTGTACCTCGACAGCAACAAGGTGAAGATGATTATCAGAAGCAAGACGATCATGTCAGTGATGACAATAGAGTCAGTAGTGGTGTTAGTGCAACAAGAAAGAGGGCAAGAGGAGGCGCACTCATGGAGGGGTCGCGGTGCAGTCGCGTTAATGGAAGAGGATGGAGGTGTTTCCAACAAACACTTGTTGGGTACTCTCTTTGCGAGCATCACTTGGGCAAGGGTAGGTTGAGGAGCATGAATAGTGTTAGAAGCCGGTCGACGGCAGTGGCAACTACTACTACTACCACTTCTACTATTACTAGAGCTGCTTCCACTGAGACTGAGTTGAAGCAATTATCAGTATCTAGAATATTATCCTTGGAGTCGCCGCGGAAAGAAGACACCAAAGCTATTGTGCTGCTGGATGATCGTGATATCGGTGAAGGTAAAGGAAATGAAGGTGAAGAGGAAGAGAAGAAGCCATTGCCGGTTACAAAGAAGCGGATGAAGCTTGGGATTGTTAAAGCCCGATCCATGAGCAGCTTGCTAGGTCAAACAAATAGTAGTGCAATTTCAGTAGTGGCTGATGAGGATGAGGATAATAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

399

Amino Acids

43.89

Weight (kDa)

9.09

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 244 - 284 1e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 656, 674
AccB1I GGYRCC 2 cut(s) 22, 52
AccB7I CCANNNNNTGG 1 cut(s) 461
AccI GTMKAC 1 cut(s) 863
AccII CGCG 3 cut(s) 739, 750, 976
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 5 cut(s) 121, 739, 974, 976, 1087
AclWI GGATC 4 cut(s) 14, 59, 122, 1110
AfaI GTAC 2 cut(s) 609, 797
AfiI CCNNNNNNNGG 2 cut(s) 461, 865
AflIII ACRYGT 1 cut(s) 202
AgsI TTSAA 4 cut(s) 316, 476, 499, 934
AjiI CACGTC 1 cut(s) 205
AluBI AGCT 7 cut(s) 287, 458, 516, 911, 995, 1096, 1128
AluI AGCT 7 cut(s) 287, 458, 516, 911, 995, 1096, 1128
Alw26I GTCTC 3 cut(s) 45, 211, 917
AlwI GGATC 4 cut(s) 14, 59, 122, 1110
AlwNI CAGNNNCTG 1 cut(s) 461
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 4 cut(s) 251, 911, 1003, 1125
AseI ATTAAT 1 cut(s) 416
AspLEI GCGC 2 cut(s) 303, 722
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 5 cut(s) 84, 443, 638, 1037, 1058
BaeGI GKGCMC 1 cut(s) 27
BamHI GGATCC 1 cut(s) 6
BanI GGYRCC 2 cut(s) 22, 52
BbsI GAAGAC 2 cut(s) 596, 990
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 4 cut(s) 238, 898, 990, 1137
BccI CCATC 3 cut(s) 183, 189, 759
BceAI ACGGC 2 cut(s) 175, 882
BclI TGATCA 1 cut(s) 559
BcoDI GTCTC 3 cut(s) 45, 211, 917
BfaI CTAG 4 cut(s) 441, 906, 951, 1133
BfoI RGCGCY 1 cut(s) 304
BisI GCNGC 5 cut(s) 252, 912, 974, 1004, 1126
BlsI GCNGC 5 cut(s) 253, 913, 975, 1005, 1127
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 3 cut(s) 8, 24, 54
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 238, 820
BmuI ACTGGG 1 cut(s) 143
BpiI GAAGAC 2 cut(s) 596, 990
BplI GAGNNNNNCTC 2 cut(s) 708, 740
BpmI CTGGAG 1 cut(s) 276
Bpu10I CCTNAGC 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 636
BsaJI CCNNGG 3 cut(s) 102, 963, 974
BsaWI WCCGGW 2 cut(s) 9, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 461, 865
Bse118I RCCGGY 2 cut(s) 858, 1072
Bse1I ACTGG 1 cut(s) 149
Bse3DI GCAATG 1 cut(s) 1067
Bse8I GATNNNNATC 1 cut(s) 636
BseAI TCCGGA 1 cut(s) 9
BseDI CCNNGG 3 cut(s) 102, 963, 974
BseGI GGATG 8 cut(s) 175, 181, 379, 562, 770, 1015, 1095, 1183
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 2 cut(s) 461, 865
BseMI GCAATG 1 cut(s) 1067
BseMII CTCAG 3 cut(s) 200, 912, 918
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 4 cut(s) 62, 366, 729, 850
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 4 cut(s) 238, 898, 990, 1137
BseYI CCCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 763
Bsh1236I CGCG 3 cut(s) 739, 750, 976
Bsh1285I CGRYCG 2 cut(s) 216, 863
BshFI GGCC 1 cut(s) 174
BshNI GGYRCC 2 cut(s) 22, 52
BsiEI CGRYCG 2 cut(s) 216, 863
BsiSI CCGG 4 cut(s) 10, 50, 859, 1073
BslI CCNNNNNNNGG 2 cut(s) 461, 865
BsmAI GTCTC 3 cut(s) 45, 211, 917
BsmBI CGTCTC 2 cut(s) 45, 211
BsnI GGCC 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 27
Bsp13I TCCGGA 1 cut(s) 9
BspACI CCGC 5 cut(s) 121, 739, 974, 976, 1087
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 3 cut(s) 199, 913, 919
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 3 cut(s) 739, 750, 976
BspLI GGNNCC 3 cut(s) 8, 24, 54
BspPI GGATC 4 cut(s) 14, 59, 122, 1110
BspT107I GGYRCC 2 cut(s) 22, 52
BsrDI GCAATG 1 cut(s) 1067
BsrFI RCCGGY 2 cut(s) 858, 1072
BsrI ACTGG 1 cut(s) 149
BssAI RCCGGY 2 cut(s) 858, 1072
BssECI CCNNGG 3 cut(s) 102, 963, 974
BssT1I CCWWGG 1 cut(s) 963
Bst6I CTCTTC 4 cut(s) 502, 754, 1044, 1050
BstC8I GCNNGC 4 cut(s) 226, 285, 809, 1130
BstDEI CTNAG 3 cut(s) 186, 921, 927
BstDSI CCRYGG 2 cut(s) 102, 974
BstF5I GGATG 8 cut(s) 175, 181, 379, 562, 770, 1015, 1095, 1183
BstFNI CGCG 3 cut(s) 739, 750, 976
BstH2I RGCGCY 1 cut(s) 304
BstHHI GCGC 2 cut(s) 303, 722
BstMAI GTCTC 3 cut(s) 45, 211, 917
BstMCI CGRYCG 2 cut(s) 216, 863
BstMWI GCNNNNNNNGC 1 cut(s) 1093
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 3 cut(s) 739, 750, 976
BstV1I GCAGC 4 cut(s) 238, 898, 990, 1137
BstV2I GAAGAC 2 cut(s) 596, 990
BstX2I RGATCY 2 cut(s) 6, 64
BstYI RGATCY 2 cut(s) 6, 64
BsuRI GGCC 1 cut(s) 174
BtgI CCRYGG 2 cut(s) 102, 974
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 8 cut(s) 175, 181, 379, 562, 770, 1015, 1095, 1183
BtsI GCAGTG 1 cut(s) 876
BtsIMutI CAGTG 3 cut(s) 667, 876, 918
Cac8I GCNNGC 4 cut(s) 226, 285, 809, 1130
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 2 cut(s) 303, 722
Cfr10I RCCGGY 2 cut(s) 858, 1072
Cfr13I GGNCC 1 cut(s) 172
Cfr42I CCGCGG 1 cut(s) 977
Csp6I GTAC 2 cut(s) 608, 796
CviAII CATG 6 cut(s) 125, 167, 656, 727, 841, 1120
CviQI GTAC 2 cut(s) 608, 796
DdeI CTNAG 3 cut(s) 186, 921, 927
DrdI GACNNNNNNGTC 2 cut(s) 656, 674
DseDI GACNNNNNNGTC 2 cut(s) 656, 674
Eam1104I CTCTTC 4 cut(s) 502, 754, 1044, 1050
EarI CTCTTC 4 cut(s) 502, 754, 1044, 1050
Eco130I CCWWGG 1 cut(s) 963
Eco32I GATATC 1 cut(s) 1021
EcoRV GATATC 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 963
Esp3I CGTCTC 2 cut(s) 45, 211
FaeI CATG 6 cut(s) 128, 170, 659, 730, 844, 1123
FaiI YATR 7 cut(s) 126, 168, 398, 657, 728, 842, 1121
FatI CATG 6 cut(s) 124, 166, 655, 726, 840, 1119
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 1 cut(s) 863
Fnu4HI GCNGC 5 cut(s) 252, 912, 974, 1004, 1126
FokI GGATG 8 cut(s) 162, 168, 386, 569, 777, 1022, 1102, 1190
Fsp4HI GCNGC 5 cut(s) 252, 912, 974, 1004, 1126
FspBI CTAG 4 cut(s) 441, 906, 951, 1133
GlaI GCGC 2 cut(s) 302, 721
GluI GCNGC 5 cut(s) 252, 912, 974, 1004, 1126
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 304
HaeIII GGCC 1 cut(s) 174
HapII CCGG 4 cut(s) 10, 50, 859, 1073
HhaI GCGC 2 cut(s) 303, 722
Hin1II CATG 6 cut(s) 128, 170, 659, 730, 844, 1123
Hin6I GCGC 2 cut(s) 301, 720
HinP1I GCGC 2 cut(s) 301, 720
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HindIII AAGCTT 2 cut(s) 285, 1094
HinfI GANTC 3 cut(s) 338, 675, 968
HpaII CCGG 4 cut(s) 10, 50, 859, 1073
HphI GGTGA 5 cut(s) 84, 443, 638, 1037, 1058
Hpy166II GTNNAC 2 cut(s) 608, 864
Hpy188I TCNGA 7 cut(s) 64, 196, 218, 360, 542, 564, 642
Hpy188III TCNNGA 5 cut(s) 10, 342, 520, 951, 1016
Hpy8I GTNNAC 2 cut(s) 608, 864
Hpy99I CGWCG 1 cut(s) 868
HpyAV CCTTC 4 cut(s) 37, 355, 1022, 1037
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 5 cut(s) 109, 407, 695, 744, 1154
HpyF10VI GCNNNNNNNGC 1 cut(s) 1093
HpyF3I CTNAG 3 cut(s) 186, 921, 927
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 6 cut(s) 128, 170, 659, 730, 844, 1123
HspAI GCGC 2 cut(s) 301, 720
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 559
KspI CCGCGG 1 cut(s) 977
LmnI GCTCC 4 cut(s) 298, 513, 527, 837
Lsp1109I GCAGC 4 cut(s) 238, 898, 990, 1137
LweI GCATC 2 cut(s) 238, 820
MaeI CTAG 4 cut(s) 441, 906, 951, 1133
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 1075
MflI RGATCY 2 cut(s) 6, 64
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 2 cut(s) 938, 1155
MlyI GAGTC 2 cut(s) 684, 977
MmeI TCCRAC 2 cut(s) 168, 802
MroI TCCGGA 1 cut(s) 9
MseI TTAA 3 cut(s) 416, 753, 1107
MslI CAYNNNNRTG 2 cut(s) 123, 660
MspA1I CMGCKG 1 cut(s) 976
MspI CCGG 4 cut(s) 10, 50, 859, 1073
MvnI CGCG 3 cut(s) 739, 750, 976
MwoI GCNNNNNNNGC 1 cut(s) 1093
NlaIII CATG 6 cut(s) 128, 170, 659, 730, 844, 1123
NlaIV GGNNCC 3 cut(s) 8, 24, 54
PfeI GAWTC 1 cut(s) 338
PflMI CCANNNNNTGG 1 cut(s) 461
PkrI GCNGC 5 cut(s) 253, 913, 975, 1005, 1127
Ple19I CGATCG 1 cut(s) 216
PleI GAGTC 2 cut(s) 683, 976
PpsI GAGTC 2 cut(s) 683, 976
PshBI ATTAAT 1 cut(s) 416
PspFI CCCAGC 1 cut(s) 454
PspN4I GGNNCC 3 cut(s) 8, 24, 54
PspPI GGNCC 1 cut(s) 172
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 2 cut(s) 6, 64
PvuI CGATCG 1 cut(s) 216
RsaI GTAC 2 cut(s) 609, 797
RsaNI GTAC 2 cut(s) 608, 796
RseI CAYNNNNRTG 2 cut(s) 123, 660
SacII CCGCGG 1 cut(s) 977
SalI GTCGAC 1 cut(s) 862
SaqAI TTAA 3 cut(s) 416, 753, 1107
SatI GCNGC 5 cut(s) 252, 912, 974, 1004, 1126
Sau96I GGNCC 1 cut(s) 172
SchI GAGTC 2 cut(s) 684, 977
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 2 cut(s) 238, 820
Sfr303I CCGCGG 1 cut(s) 977
SgrBI CCGCGG 1 cut(s) 977
SmiMI CAYNNNNRTG 2 cut(s) 123, 660
Sse9I AATT 2 cut(s) 938, 1155
SsiI CCGC 5 cut(s) 121, 739, 974, 976, 1087
SspI AATATT 1 cut(s) 957
SspMI CTAG 4 cut(s) 441, 906, 951, 1133
StyI CCWWGG 1 cut(s) 963
TaiI ACGT 1 cut(s) 207
TaqI TCGA 3 cut(s) 519, 613, 863
TasI AATT 2 cut(s) 938, 1155
TauI GCSGC 1 cut(s) 976
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 3 cut(s) 416, 753, 1107
Tru9I TTAA 3 cut(s) 416, 753, 1107
TscAI CASTG 3 cut(s) 667, 876, 925
TseI GCWGC 4 cut(s) 251, 911, 1003, 1125
TspDTI ATGAA 5 cut(s) 393, 596, 857, 1056, 1106
TspRI CASTG 3 cut(s) 667, 876, 925
Van91I CCANNNNNTGG 1 cut(s) 461
VspI ATTAAT 1 cut(s) 416
XbaI TCTAGA 1 cut(s) 950
XmiI GTMKAC 1 cut(s) 863
XspI CTAG 4 cut(s) 441, 906, 951, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.