RchiOBHm_Chr3g0479411

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0011978
Physical Location & Seq
Forward (+)
25267531 .. 25269780
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44450

Sequence Viewer

Length: 1098 bp
ATGAGGATCCGGAAAAGACAGGTCCCGTTGCCCCTCTCTTCTCTCTCTCCCGTGCCTCTACCAGATCCCCACCTCCTCAACCTCAACCACTGCTCATCATCACCTCCTCTGGTGCAACAACAACAACAACAACAACGTATCCACCACGATCCAAAACCCTCCCAACGACTTGATCATGTGGGCCCTCTCCCTCATCCTCAGCCGTCTGATCATCCCAATCGCCCCCGGCCGATTGGAGCAGCGAGCGACGACGGCTGTGATTTTTCCGGGGAGCTGAAGAAGAGAAATTACTGCTCGGAGACATTGAAAGGGGAAGACGAAGATGGATCAAGAGATCAGGCAGAGGAGAAGGGTAATGATACCAGGAAGGGAAGCACATTGGGTGCAGAAACTGTATCTGGGTTTCCTTCAGAATCATCAAGTTCTTCTCATCGAGTTCTTGGGAGATGGTTTGAGGGAGAGAAAGCGTTCCCATTCAAGAAGCGAAGAGGGAGCTTCGGGGAGGCGACGAGAATGGAGAAGGAGAAGAAATTAACGCAGACCAAGATGGACAAGAAATGTGATGGTCAACAAAGTGATGATAAGAAGCAAGAAGATAGAGTAATTACGAAAGAAAGCCTCGACCATACTGTTGGTAGTGGTACGAGTCCAAAGAAGAGAGCAAGAGGCAATGCAATAATGGAGGGGTCGAGGTGCAGTCGAGTTAACGGAAGAGGATGGAGGTGTTGTCAACAGACACTTGTCGGGTACTCTCTTTGCGAGCATCACTTGGGCAAAGGAAGGCTTAGGAGCTTGAACAGCGTCCGAAGTCGCTCTTTTGCGAGTTCTGTTGGATCCATAGAGACCAAGTCCAAGCAATTATCAAAACCCTCGTGTTTGGAGCTGCCAAAGGAAGAGGAGCGTAAACCTGATACGCTACTAGATGTCAATCATCATGGTGAAGATCAACGACACGAAAATGAATACGGTGAAGAGAAAAAGCCGTTGGTGGTTACGAAGAAGAGAATGAAACTCGGTATTGTAAAGGCACGATCCATGAGCAGCTTGCTTGGGCAAACGAACAGTGCAACTGCAGTGGATGATGAGAATAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

365

Amino Acids

40.77

Weight (kDa)

9.35

Isoelectric Point (pI)

53.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 228 - 268 2.7e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 9
AclWI GGATC 7 cut(s) 14, 59, 143, 334, 828, 841, 1026
AcoI YGGCCR 1 cut(s) 227
AcuI CTGAAG 2 cut(s) 296, 393
AfaI GTAC 2 cut(s) 643, 749
AgsI TTSAA 3 cut(s) 307, 478, 796
AjnI CCWGG 1 cut(s) 362
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AluBI AGCT 5 cut(s) 274, 495, 792, 883, 1044
AluI AGCT 5 cut(s) 274, 495, 792, 883, 1044
Alw26I GTCTC 2 cut(s) 293, 836
AlwI GGATC 7 cut(s) 14, 59, 143, 334, 828, 841, 1026
AlwNI CAGNNNCTG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 2 cut(s) 181, 227
ApaI GGGCCC 1 cut(s) 185
ApeKI GCWGC 3 cut(s) 239, 883, 1041
Asp700I GAANNNNTTC 1 cut(s) 467
AspS9I GGNCC 3 cut(s) 22, 181, 182
AsuC2I CCSGG 2 cut(s) 226, 268
AsuHPI GGTGA 3 cut(s) 93, 950, 980
AvaII GGWCC 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 185
BamHI GGATCC 2 cut(s) 6, 833
BanII GRGCYC 1 cut(s) 185
BarI GAAGNNNNNNTAC 2 cut(s) 272, 304
BauI CACGAG 1 cut(s) 871
BbsI GAAGAC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 251, 870, 1053
BccI CCATC 5 cut(s) 317, 441, 541, 557, 711
BceAI ACGGC 3 cut(s) 187, 268, 967
BciT130I CCWGG 1 cut(s) 364
BciVI GTATCC 1 cut(s) 149
BclI TGATCA 2 cut(s) 172, 208
BcnI CCSGG 2 cut(s) 226, 268
BcoDI GTCTC 2 cut(s) 293, 836
BfaI CTAG 1 cut(s) 920
BfmI CTRYAG 1 cut(s) 1071
BfuI GTATCC 1 cut(s) 149
BisI GCNGC 3 cut(s) 240, 884, 1042
BlsI GCNGC 3 cut(s) 241, 885, 1043
Bme1390I CCNGG 3 cut(s) 226, 268, 364
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 3 cut(s) 22, 181, 182
BmiI GGNNCC 4 cut(s) 8, 24, 183, 835
BmrFI CCNGG 3 cut(s) 226, 268, 364
BmsI GCATC 1 cut(s) 772
BoxI GACNNNNGTC 1 cut(s) 740
BpiI GAAGAC 1 cut(s) 321
Bpu10I CCTNAGC 2 cut(s) 198, 785
BpuMI CCSGG 2 cut(s) 226, 268
BsaBI GATNNNNATC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 2 cut(s) 224, 267
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 712, 742
Bse3DI GCAATG 1 cut(s) 676
Bse8I GATNNNNATC 1 cut(s) 927
BseAI TCCGGA 1 cut(s) 9
BseBI CCWGG 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 224, 267
BseGI GGATG 4 cut(s) 193, 211, 722, 1084
BseJI GATNNNNATC 1 cut(s) 927
BseMI GCAATG 1 cut(s) 676
BseMII CTCAG 1 cut(s) 212
BseRI GAGGAG 4 cut(s) 65, 96, 359, 911
BseSI GKGCMC 1 cut(s) 185
BseX3I CGGCCG 1 cut(s) 227
BseXI GCAGC 3 cut(s) 251, 870, 1053
BsgI GTGCAG 2 cut(s) 405, 715
Bsh1285I CGRYCG 1 cut(s) 230
BshFI GGCC 2 cut(s) 183, 229
BsiEI CGRYCG 1 cut(s) 230
BsiSI CCGG 3 cut(s) 10, 226, 267
BslFI GGGAC 1 cut(s) 8
BsmAI GTCTC 2 cut(s) 293, 836
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 2 cut(s) 183, 229
Bso31I GGTCTC 1 cut(s) 836
Bsp120I GGGCCC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 185
Bsp13I TCCGGA 1 cut(s) 9
BspANI GGCC 2 cut(s) 183, 229
BspCNI CTCAG 1 cut(s) 211
BspEI TCCGGA 1 cut(s) 9
BspLI GGNNCC 4 cut(s) 8, 24, 183, 835
BspMAI CTGCAG 1 cut(s) 1075
BspPI GGATC 7 cut(s) 14, 59, 143, 334, 828, 841, 1026
BspTNI GGTCTC 1 cut(s) 836
BsrDI GCAATG 1 cut(s) 676
BssECI CCNNGG 2 cut(s) 224, 267
BssSI CACGAG 1 cut(s) 871
Bst2BI CACGAG 1 cut(s) 871
Bst2UI CCWGG 1 cut(s) 364
Bst4CI ACNGT 4 cut(s) 394, 631, 968, 1064
Bst6I CTCTTC 8 cut(s) 43, 275, 481, 650, 706, 888, 966, 995
BstC8I GCNNGC 3 cut(s) 244, 761, 1046
BstDEI CTNAG 2 cut(s) 198, 785
BstF5I GGATG 4 cut(s) 193, 211, 722, 1084
BstMAI GTCTC 2 cut(s) 293, 836
BstMCI CGRYCG 1 cut(s) 230
BstMWI GCNNNNNNNGC 2 cut(s) 252, 798
BstNI CCWGG 1 cut(s) 364
BstPAI GACNNNNGTC 1 cut(s) 740
BstSCI CCNGG 3 cut(s) 224, 266, 362
BstSFI CTRYAG 1 cut(s) 1071
BstSLI GKGCMC 1 cut(s) 185
BstV1I GCAGC 3 cut(s) 251, 870, 1053
BstV2I GAAGAC 1 cut(s) 321
BstX2I RGATCY 3 cut(s) 6, 64, 833
BstXI CCANNNNNNTGG 1 cut(s) 632
BstYI RGATCY 3 cut(s) 6, 64, 833
BstZI CGGCCG 1 cut(s) 227
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 2 cut(s) 183, 229
BtsCI GGATG 4 cut(s) 193, 211, 722, 1084
BtsI GCAGTG 2 cut(s) 88, 1080
BtsIMutI CAGTG 3 cut(s) 88, 1069, 1080
Cac8I GCNNGC 3 cut(s) 244, 761, 1046
CaiI CAGNNNCTG 1 cut(s) 392
Cfr13I GGNCC 3 cut(s) 22, 181, 182
CseI GACGC 1 cut(s) 790
Csp6I GTAC 2 cut(s) 642, 748
CspCI CAANNNNNGTGG 2 cut(s) 1056, 1091
CviAII CATG 3 cut(s) 176, 935, 1036
CviQI GTAC 2 cut(s) 642, 748
DdeI CTNAG 2 cut(s) 198, 785
EaeI YGGCCR 1 cut(s) 227
EagI CGGCCG 1 cut(s) 227
Eam1104I CTCTTC 8 cut(s) 43, 275, 481, 650, 706, 888, 966, 995
EarI CTCTTC 8 cut(s) 43, 275, 481, 650, 706, 888, 966, 995
EclXI CGGCCG 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 836
Eco47I GGWCC 1 cut(s) 22
Eco52I CGGCCG 1 cut(s) 227
Eco57I CTGAAG 2 cut(s) 296, 393
EcoO109I RGGNCCY 2 cut(s) 22, 182
EcoRII CCWGG 1 cut(s) 362
EcoT38I GRGCYC 1 cut(s) 185
FaeI CATG 3 cut(s) 179, 938, 1039
FaiI YATR 5 cut(s) 177, 627, 839, 936, 1037
FalI AAGNNNNNCTT 4 cut(s) 768, 800, 799, 831
FaqI GGGAC 1 cut(s) 8
FatI CATG 3 cut(s) 175, 934, 1035
FbaI TGATCA 2 cut(s) 172, 208
Fnu4HI GCNGC 3 cut(s) 240, 884, 1042
FokI GGATG 4 cut(s) 180, 198, 729, 1091
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 3 cut(s) 240, 884, 1042
FspBI CTAG 1 cut(s) 920
GluI GCNGC 3 cut(s) 240, 884, 1042
HaeIII GGCC 2 cut(s) 183, 229
HapII CCGG 3 cut(s) 10, 226, 267
HgaI GACGC 1 cut(s) 790
Hin1II CATG 3 cut(s) 179, 938, 1039
HincII GTYRAC 3 cut(s) 569, 706, 731
HindII GTYRAC 3 cut(s) 569, 706, 731
HinfI GANTC 2 cut(s) 413, 646
HpaI GTTAAC 1 cut(s) 706
HpaII CCGG 3 cut(s) 10, 226, 267
HphI GGTGA 3 cut(s) 93, 950, 980
Hpy166II GTNNAC 4 cut(s) 569, 706, 731, 905
Hpy188I TCNGA 4 cut(s) 208, 298, 412, 806
Hpy188III TCNNGA 3 cut(s) 10, 330, 478
Hpy8I GTNNAC 4 cut(s) 569, 706, 731, 905
Hpy99I CGWCG 3 cut(s) 251, 254, 511
HpyAV CCTTC 5 cut(s) 343, 361, 417, 514, 774
HpyCH4III ACNGT 4 cut(s) 394, 631, 968, 1064
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 6 cut(s) 115, 386, 674, 696, 1067, 1073
HpyF10VI GCNNNNNNNGC 2 cut(s) 252, 798
HpyF3I CTNAG 2 cut(s) 198, 785
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 3 cut(s) 179, 938, 1039
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 2 cut(s) 172, 208
KspAI GTTAAC 1 cut(s) 706
LmnI GCTCC 6 cut(s) 236, 271, 492, 789, 880, 898
Lsp1109I GCAGC 3 cut(s) 251, 870, 1053
LweI GCATC 1 cut(s) 772
MaeI CTAG 1 cut(s) 920
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 991
MflI RGATCY 3 cut(s) 6, 64, 833
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 4 cut(s) 286, 530, 603, 857
MlyI GAGTC 1 cut(s) 655
MmeI TCCRAC 1 cut(s) 811
MroI TCCGGA 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 2 cut(s) 533, 705
MslI CAYNNNNRTG 2 cut(s) 936, 957
MspI CCGG 3 cut(s) 10, 226, 267
MspR9I CCNGG 3 cut(s) 226, 268, 364
MvaI CCWGG 1 cut(s) 364
MwoI GCNNNNNNNGC 2 cut(s) 252, 798
NciI CCSGG 2 cut(s) 226, 268
NlaIII CATG 3 cut(s) 179, 938, 1039
NlaIV GGNNCC 4 cut(s) 8, 24, 183, 835
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 847
PkrI GCNGC 3 cut(s) 241, 885, 1043
PleI GAGTC 1 cut(s) 654
PpsI GAGTC 1 cut(s) 654
PpuMI RGGWCCY 1 cut(s) 22
PshAI GACNNNNGTC 1 cut(s) 740
Psp5II RGGWCCY 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 362
PspN4I GGNNCC 4 cut(s) 8, 24, 183, 835
PspOMI GGGCCC 1 cut(s) 181
PspPI GGNCC 3 cut(s) 22, 181, 182
PspPPI RGGWCCY 1 cut(s) 22
PstI CTGCAG 1 cut(s) 1075
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 3 cut(s) 6, 64, 833
PsyI GACNNNGTC 1 cut(s) 847
RsaI GTAC 2 cut(s) 643, 749
RsaNI GTAC 2 cut(s) 642, 748
RseI CAYNNNNRTG 2 cut(s) 936, 957
SaqAI TTAA 2 cut(s) 533, 705
SatI GCNGC 3 cut(s) 240, 884, 1042
Sau96I GGNCC 3 cut(s) 22, 181, 182
SchI GAGTC 1 cut(s) 655
ScrFI CCNGG 3 cut(s) 226, 268, 364
SduI GDGCHC 1 cut(s) 185
SfaNI GCATC 1 cut(s) 772
SfcI CTRYAG 1 cut(s) 1071
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 2 cut(s) 936, 957
Sse9I AATT 4 cut(s) 286, 530, 603, 857
SspMI CTAG 1 cut(s) 920
StyD4I CCNGG 3 cut(s) 224, 266, 362
TaaI ACNGT 4 cut(s) 394, 631, 968, 1064
TaiI ACGT 1 cut(s) 139
TaqI TCGA 4 cut(s) 433, 621, 689, 700
TasI AATT 4 cut(s) 286, 530, 603, 857
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 2 cut(s) 533, 705
Tru9I TTAA 2 cut(s) 533, 705
TscAI CASTG 3 cut(s) 95, 1069, 1080
TseI GCWGC 3 cut(s) 239, 883, 1041
TspDTI ATGAA 2 cut(s) 975, 1022
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 3 cut(s) 95, 1069, 1080
Tth111I GACNNNGTC 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.