Rh3BG258900

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011978
Physical Location & Seq
Forward (+)
25089201 .. 25090225
1025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG258900.1

Sequence Viewer

Length: 444 bp
ATGAGGATCCGGAAAAGACAGGTCCCGTTACCCCTCTCTTCTCTCTCGCCCGTGCCTCTACCAGATCCCCACCTCCTCAACCTCAACCACTGCTCATCATCACCTCCCCTGGTGCAACAACAACAACAACAACAACGTATCCACCACGATCCAAAACCCTCCCAACGACTTGATCATGTGGGCCCTCTCCCACATCCTCAGCCGTCTGATCATCCCAATCGCCCCCGGCCGATTGGAGCAGCGAGCAACGACGGCTGTGATTTTTCCGGGGAGCTGAAGAAGGGAAATTACTGCTCGGAGACATTGAAAGGGGAAGACGACGATGGATCAAGAGATCAGGCAGAGGAGAAGGGTAATGATACCAGGAAGGGAAGCACATTGGGTGCAGAAACTGTATCTGGGTTTCCTTCAGAATCATCAAGTTCTTCTCATCGAGGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

147

Amino Acids

16.03

Weight (kDa)

6.7

Isoelectric Point (pI)

55.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 9
AclWI GGATC 4 cut(s) 14, 59, 143, 334
AcoI YGGCCR 1 cut(s) 227
AcuI CTGAAG 2 cut(s) 296, 393
AgsI TTSAA 1 cut(s) 307
AjnI CCWGG 2 cut(s) 108, 362
AluBI AGCT 1 cut(s) 274
AluI AGCT 1 cut(s) 274
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 4 cut(s) 14, 59, 143, 334
AlwNI CAGNNNCTG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 2 cut(s) 181, 227
ApaI GGGCCC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 239
AspS9I GGNCC 3 cut(s) 22, 181, 182
AsuC2I CCSGG 2 cut(s) 226, 268
AsuHPI GGTGA 1 cut(s) 93
AvaII GGWCC 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 185
BamHI GGATCC 1 cut(s) 6
BanII GRGCYC 1 cut(s) 185
BarI GAAGNNNNNNTAC 2 cut(s) 272, 304
BbsI GAAGAC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 1 cut(s) 251
BccI CCATC 1 cut(s) 317
BceAI ACGGC 2 cut(s) 187, 268
BciT130I CCWGG 2 cut(s) 110, 364
BciVI GTATCC 1 cut(s) 149
BclI TGATCA 2 cut(s) 172, 208
BcnI CCSGG 2 cut(s) 226, 268
BcoDI GTCTC 1 cut(s) 293
BfuI GTATCC 1 cut(s) 149
BisI GCNGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 241
Bme1390I CCNGG 4 cut(s) 110, 226, 268, 364
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 3 cut(s) 22, 181, 182
BmiI GGNNCC 3 cut(s) 8, 24, 183
BmrFI CCNGG 4 cut(s) 110, 226, 268, 364
BpiI GAAGAC 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 198
BpuMI CCSGG 2 cut(s) 226, 268
BsaJI CCNNGG 3 cut(s) 108, 224, 267
BsaWI WCCGGW 1 cut(s) 9
BseAI TCCGGA 1 cut(s) 9
BseBI CCWGG 2 cut(s) 110, 364
BseDI CCNNGG 3 cut(s) 108, 224, 267
BseGI GGATG 2 cut(s) 193, 211
BseMII CTCAG 1 cut(s) 212
BseRI GAGGAG 2 cut(s) 65, 359
BseSI GKGCMC 1 cut(s) 185
BseX3I CGGCCG 1 cut(s) 227
BseXI GCAGC 1 cut(s) 251
BsgI GTGCAG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 230
BshFI GGCC 2 cut(s) 183, 229
BsiEI CGRYCG 1 cut(s) 230
BsiSI CCGG 3 cut(s) 10, 226, 267
BslFI GGGAC 1 cut(s) 8
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 2 cut(s) 183, 229
Bsp120I GGGCCC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 185
Bsp13I TCCGGA 1 cut(s) 9
Bsp143I GATC 7 cut(s) 6, 64, 148, 172, 208, 326, 334
BspANI GGCC 2 cut(s) 183, 229
BspCNI CTCAG 1 cut(s) 211
BspEI TCCGGA 1 cut(s) 9
BspLI GGNNCC 3 cut(s) 8, 24, 183
BspPI GGATC 4 cut(s) 14, 59, 143, 334
BssECI CCNNGG 3 cut(s) 108, 224, 267
BssMI GATC 7 cut(s) 6, 64, 148, 172, 208, 326, 334
Bst2UI CCWGG 2 cut(s) 110, 364
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 1 cut(s) 198
BstF5I GGATG 2 cut(s) 193, 211
BstKTI GATC 7 cut(s) 9, 67, 151, 175, 211, 329, 337
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 7 cut(s) 6, 64, 148, 172, 208, 326, 334
BstMCI CGRYCG 1 cut(s) 230
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNI CCWGG 2 cut(s) 110, 364
BstSCI CCNGG 4 cut(s) 108, 224, 266, 362
BstSLI GKGCMC 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 251
BstV2I GAAGAC 1 cut(s) 321
BstX2I RGATCY 2 cut(s) 6, 64
BstYI RGATCY 2 cut(s) 6, 64
BstZI CGGCCG 1 cut(s) 227
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 2 cut(s) 183, 229
BtsCI GGATG 2 cut(s) 193, 211
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 1 cut(s) 244
CaiI CAGNNNCTG 1 cut(s) 392
Cfr13I GGNCC 3 cut(s) 22, 181, 182
CviAII CATG 1 cut(s) 176
CviJI RGCY 5 cut(s) 183, 202, 229, 255, 274
CviKI_1 RGCY 5 cut(s) 183, 202, 229, 255, 274
DdeI CTNAG 1 cut(s) 198
DpnI GATC 7 cut(s) 8, 66, 150, 174, 210, 328, 336
DpnII GATC 7 cut(s) 6, 64, 148, 172, 208, 326, 334
EaeI YGGCCR 1 cut(s) 227
EagI CGGCCG 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 43
EarI CTCTTC 1 cut(s) 43
EclXI CGGCCG 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 22
Eco52I CGGCCG 1 cut(s) 227
Eco57I CTGAAG 2 cut(s) 296, 393
EcoO109I RGGNCCY 2 cut(s) 22, 182
EcoRII CCWGG 2 cut(s) 108, 362
EcoT38I GRGCYC 1 cut(s) 185
FaeI CATG 1 cut(s) 179
FaiI YATR 1 cut(s) 177
FaqI GGGAC 1 cut(s) 8
FatI CATG 1 cut(s) 175
FbaI TGATCA 2 cut(s) 172, 208
Fnu4HI GCNGC 1 cut(s) 240
FokI GGATG 2 cut(s) 180, 198
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 240
HaeIII GGCC 2 cut(s) 183, 229
HapII CCGG 3 cut(s) 10, 226, 267
Hin1II CATG 1 cut(s) 179
HinfI GANTC 1 cut(s) 413
HpaII CCGG 3 cut(s) 10, 226, 267
HphI GGTGA 1 cut(s) 93
Hpy188I TCNGA 3 cut(s) 208, 298, 412
Hpy188III TCNNGA 2 cut(s) 10, 330
Hpy99I CGWCG 2 cut(s) 254, 323
HpyAV CCTTC 4 cut(s) 274, 343, 361, 417
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 2 cut(s) 115, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 1 cut(s) 179
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 2 cut(s) 172, 208
Kzo9I GATC 7 cut(s) 6, 64, 148, 172, 208, 326, 334
LmnI GCTCC 2 cut(s) 236, 271
Lsp1109I GCAGC 1 cut(s) 251
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 27
MalI GATC 7 cut(s) 8, 66, 150, 174, 210, 328, 336
MboI GATC 7 cut(s) 6, 64, 148, 172, 208, 326, 334
MboII GAAGA 4 cut(s) 30, 289, 326, 417
MflI RGATCY 2 cut(s) 6, 64
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 1 cut(s) 286
MroI TCCGGA 1 cut(s) 9
MspI CCGG 3 cut(s) 10, 226, 267
MspR9I CCNGG 4 cut(s) 110, 226, 268, 364
MvaI CCWGG 2 cut(s) 110, 364
MwoI GCNNNNNNNGC 1 cut(s) 252
NciI CCSGG 2 cut(s) 226, 268
NdeII GATC 7 cut(s) 6, 64, 148, 172, 208, 326, 334
NlaIII CATG 1 cut(s) 179
NlaIV GGNNCC 3 cut(s) 8, 24, 183
PfeI GAWTC 1 cut(s) 413
PkrI GCNGC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 22
Psp5II RGGWCCY 1 cut(s) 22
Psp6I CCWGG 2 cut(s) 108, 362
PspGI CCWGG 2 cut(s) 108, 362
PspN4I GGNNCC 3 cut(s) 8, 24, 183
PspOMI GGGCCC 1 cut(s) 181
PspPI GGNCC 3 cut(s) 22, 181, 182
PspPPI RGGWCCY 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 6, 64
SatI GCNGC 1 cut(s) 240
Sau3AI GATC 7 cut(s) 6, 64, 148, 172, 208, 326, 334
Sau96I GGNCC 3 cut(s) 22, 181, 182
ScrFI CCNGG 4 cut(s) 110, 226, 268, 364
SduI GDGCHC 1 cut(s) 185
SetI ASST 7 cut(s) 24, 75, 84, 106, 139, 276, 439
SinI GGWCC 1 cut(s) 22
Sse9I AATT 1 cut(s) 286
StyD4I CCNGG 4 cut(s) 108, 224, 266, 362
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 433
TasI AATT 1 cut(s) 286
TfiI GAWTC 1 cut(s) 413
TscAI CASTG 1 cut(s) 95
TseI GCWGC 1 cut(s) 239
TspRI CASTG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.