Rh3DG250600

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0011978
Physical Location & Seq
Forward (+)
24680412 .. 24682193
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG250600.1

Sequence Viewer

Length: 1098 bp
ATGAGGATCCGGAAAAGACAGGTGCCGTTGCCCCTCTCTTCTCTCTCTCCCGTGCCTCTACCAGATCCCCACCTCCTCAACCTCAACCACTGCTCATCATCACCTCCCCTGGTGCAACAACAACAACAACAACAACAACGTATCCACCACGATCCAAAACCCTCCCAACGACTTGATCATGTGGGCCCTCCCCCTCATCCTCAGCCGTCTGATCATCACAATCGCCCCCGGCCGATTGGAGCAGCGAGCGACGACGGCTGTGATTTTTCCGGGGAGCTGAAGAAGAGAAATTACTGCTCGGAGACATTGAAAGGGGGAGACGAAGAGGGATCAAGAGATCAGGCAGAGGAGAAGGGTAATGATACCAGGAAGGGAAGCACATTGGGTGCAGAAACTGTATCTGGGTTTCCTTCAGAATCAAGTTCTTCTCATCGAGTTCTTGGGAGATGGTTTGAGGGAGAGAAAGCGTTCCCATTCAAGAAGCGAAGAGGGAGCTTCGGGGAGGCGACGAGAATGGAGAAGGAGAAGAAATTAACGCAGACCAAGATGGACCAGAAATGTGATGGTCAACAAAGTGATGATAAGAAGCAAGAAGATAGAGTAATTACGAAAGAAAGCCTCGACCATACTGTTGGTAGTGGTACGAGTCCAAAGAAGAGAGCAAGAGGCAATGCAATAATGGAGGGATCGAGGTGCAGTCGAGTTAACGGAAGAGGATGGAGGTGTTGTCAACAGACACTTGTCGGGTACTCTCTTTGCGAGCATCACTTGGGCAAAGGAAGGCTTAGGAGCTTGAACAGCGTCCGAAGTCGCTCTTTTGGGAGTTCTGTTGGATCCATAGAGACCAAGTCCAAGCAATTATCAAAACCCTCGTGTTTGGAGCTGCCAAAGGAAGAAGAGCGTAAACCTGATATGCTACTAGATGTCAATCATCATGGTGAAGATCAACGACACGAAAATGAATACGGTGAAGAGAAAAAGCCGTTGGTGGTTACGAAGAAGAGAATGAAACTCGGTATTGTAAAGGCACGATCCATGAGCAGCTTGCTTGGGCAAACGAACAGTGCAACTGCAGTGGATGATGAGAATAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

365

Amino Acids

40.79

Weight (kDa)

9.36

Isoelectric Point (pI)

55.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 228 - 268 2.7e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 9
AclWI GGATC 8 cut(s) 14, 59, 146, 337, 694, 828, 841, 1026
AcoI YGGCCR 1 cut(s) 230
AcuI CTGAAG 2 cut(s) 299, 396
AfaI GTAC 2 cut(s) 643, 749
AgsI TTSAA 3 cut(s) 310, 478, 796
AjnI CCWGG 2 cut(s) 108, 365
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AluBI AGCT 5 cut(s) 277, 495, 792, 883, 1044
AluI AGCT 5 cut(s) 277, 495, 792, 883, 1044
Alw26I GTCTC 3 cut(s) 296, 312, 836
AlwI GGATC 8 cut(s) 14, 59, 146, 337, 694, 828, 841, 1026
AlwNI CAGNNNCTG 1 cut(s) 395
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 2 cut(s) 184, 230
ApaI GGGCCC 1 cut(s) 188
ApeKI GCWGC 3 cut(s) 242, 883, 1041
Asp700I GAANNNNTTC 1 cut(s) 467
AspS9I GGNCC 3 cut(s) 184, 185, 550
AsuC2I CCSGG 2 cut(s) 229, 271
AsuHPI GGTGA 3 cut(s) 93, 950, 980
AvaII GGWCC 1 cut(s) 550
BaeGI GKGCMC 1 cut(s) 188
BamHI GGATCC 2 cut(s) 6, 833
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 188
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BauI CACGAG 1 cut(s) 871
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 3 cut(s) 254, 870, 1053
BccI CCATC 4 cut(s) 441, 541, 557, 711
BceAI ACGGC 4 cut(s) 10, 190, 271, 967
BciT130I CCWGG 2 cut(s) 110, 367
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 2 cut(s) 175, 211
BcnI CCSGG 2 cut(s) 229, 271
BcoDI GTCTC 3 cut(s) 296, 312, 836
BfaI CTAG 1 cut(s) 920
BfmI CTRYAG 1 cut(s) 1071
BfuI GTATCC 1 cut(s) 152
BisI GCNGC 3 cut(s) 243, 884, 1042
BlsI GCNGC 3 cut(s) 244, 885, 1043
Bme1390I CCNGG 4 cut(s) 110, 229, 271, 367
Bme18I GGWCC 1 cut(s) 550
BmgT120I GGNCC 3 cut(s) 184, 185, 550
BmiI GGNNCC 4 cut(s) 8, 24, 186, 835
BmrFI CCNGG 4 cut(s) 110, 229, 271, 367
BmsI GCATC 1 cut(s) 772
BoxI GACNNNNGTC 1 cut(s) 740
Bpu10I CCTNAGC 2 cut(s) 201, 785
BpuMI CCSGG 2 cut(s) 229, 271
BsaBI GATNNNNATC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 3 cut(s) 108, 227, 270
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 712, 742
Bse3DI GCAATG 1 cut(s) 676
Bse8I GATNNNNATC 1 cut(s) 927
BseAI TCCGGA 1 cut(s) 9
BseBI CCWGG 2 cut(s) 110, 367
BseDI CCNNGG 3 cut(s) 108, 227, 270
BseGI GGATG 3 cut(s) 196, 722, 1084
BseJI GATNNNNATC 1 cut(s) 927
BseMI GCAATG 1 cut(s) 676
BseMII CTCAG 1 cut(s) 215
BseRI GAGGAG 2 cut(s) 65, 362
BseSI GKGCMC 1 cut(s) 188
BseX3I CGGCCG 1 cut(s) 230
BseXI GCAGC 3 cut(s) 254, 870, 1053
BsgI GTGCAG 2 cut(s) 408, 715
Bsh1285I CGRYCG 1 cut(s) 233
BshFI GGCC 2 cut(s) 186, 232
BshNI GGYRCC 1 cut(s) 22
BsiEI CGRYCG 1 cut(s) 233
BsiSI CCGG 3 cut(s) 10, 229, 270
BsmAI GTCTC 3 cut(s) 296, 312, 836
BsmBI CGTCTC 1 cut(s) 312
BsnI GGCC 2 cut(s) 186, 232
Bso31I GGTCTC 1 cut(s) 836
Bsp120I GGGCCC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 9
BspANI GGCC 2 cut(s) 186, 232
BspCNI CTCAG 1 cut(s) 214
BspEI TCCGGA 1 cut(s) 9
BspLI GGNNCC 4 cut(s) 8, 24, 186, 835
BspMAI CTGCAG 1 cut(s) 1075
BspPI GGATC 8 cut(s) 14, 59, 146, 337, 694, 828, 841, 1026
BspQI GCTCTTC 1 cut(s) 891
BspT107I GGYRCC 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 836
BsrDI GCAATG 1 cut(s) 676
BssECI CCNNGG 3 cut(s) 108, 227, 270
BssSI CACGAG 1 cut(s) 871
Bst2BI CACGAG 1 cut(s) 871
Bst2UI CCWGG 2 cut(s) 110, 367
Bst4CI ACNGT 4 cut(s) 397, 631, 968, 1064
Bst6I CTCTTC 9 cut(s) 43, 278, 318, 481, 650, 706, 891, 966, 995
BstC8I GCNNGC 3 cut(s) 247, 761, 1046
BstDEI CTNAG 2 cut(s) 201, 785
BstF5I GGATG 3 cut(s) 196, 722, 1084
BstMAI GTCTC 3 cut(s) 296, 312, 836
BstMCI CGRYCG 1 cut(s) 233
BstMWI GCNNNNNNNGC 2 cut(s) 255, 798
BstNI CCWGG 2 cut(s) 110, 367
BstPAI GACNNNNGTC 1 cut(s) 740
BstSCI CCNGG 4 cut(s) 108, 227, 269, 365
BstSFI CTRYAG 1 cut(s) 1071
BstSLI GKGCMC 1 cut(s) 188
BstV1I GCAGC 3 cut(s) 254, 870, 1053
BstX2I RGATCY 3 cut(s) 6, 64, 833
BstXI CCANNNNNNTGG 1 cut(s) 632
BstYI RGATCY 3 cut(s) 6, 64, 833
BstZI CGGCCG 1 cut(s) 230
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 2 cut(s) 186, 232
BtsCI GGATG 3 cut(s) 196, 722, 1084
BtsI GCAGTG 2 cut(s) 88, 1080
BtsIMutI CAGTG 3 cut(s) 88, 1069, 1080
Cac8I GCNNGC 3 cut(s) 247, 761, 1046
CaiI CAGNNNCTG 1 cut(s) 395
Cfr13I GGNCC 3 cut(s) 184, 185, 550
CseI GACGC 1 cut(s) 790
Csp6I GTAC 2 cut(s) 642, 748
CspCI CAANNNNNGTGG 2 cut(s) 1056, 1091
CviAII CATG 3 cut(s) 179, 935, 1036
CviQI GTAC 2 cut(s) 642, 748
DdeI CTNAG 2 cut(s) 201, 785
EaeI YGGCCR 1 cut(s) 230
EagI CGGCCG 1 cut(s) 230
Eam1104I CTCTTC 9 cut(s) 43, 278, 318, 481, 650, 706, 891, 966, 995
EarI CTCTTC 9 cut(s) 43, 278, 318, 481, 650, 706, 891, 966, 995
EclXI CGGCCG 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 836
Eco47I GGWCC 1 cut(s) 550
Eco52I CGGCCG 1 cut(s) 230
Eco57I CTGAAG 2 cut(s) 299, 396
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 108, 365
EcoT38I GRGCYC 1 cut(s) 188
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 3 cut(s) 182, 938, 1039
FaiI YATR 6 cut(s) 180, 627, 839, 914, 936, 1037
FalI AAGNNNNNCTT 4 cut(s) 768, 800, 799, 831
FatI CATG 3 cut(s) 178, 934, 1035
FbaI TGATCA 2 cut(s) 175, 211
Fnu4HI GCNGC 3 cut(s) 243, 884, 1042
FokI GGATG 3 cut(s) 183, 729, 1091
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 3 cut(s) 243, 884, 1042
FspBI CTAG 1 cut(s) 920
GluI GCNGC 3 cut(s) 243, 884, 1042
HaeIII GGCC 2 cut(s) 186, 232
HapII CCGG 3 cut(s) 10, 229, 270
HgaI GACGC 1 cut(s) 790
Hin1II CATG 3 cut(s) 182, 938, 1039
HincII GTYRAC 3 cut(s) 569, 706, 731
HindII GTYRAC 3 cut(s) 569, 706, 731
HinfI GANTC 2 cut(s) 416, 646
HpaI GTTAAC 1 cut(s) 706
HpaII CCGG 3 cut(s) 10, 229, 270
HphI GGTGA 3 cut(s) 93, 950, 980
Hpy166II GTNNAC 4 cut(s) 569, 706, 731, 905
Hpy188I TCNGA 4 cut(s) 211, 301, 415, 806
Hpy188III TCNNGA 3 cut(s) 10, 333, 478
Hpy8I GTNNAC 4 cut(s) 569, 706, 731, 905
Hpy99I CGWCG 3 cut(s) 254, 257, 511
HpyAV CCTTC 5 cut(s) 346, 364, 420, 514, 774
HpyCH4III ACNGT 4 cut(s) 397, 631, 968, 1064
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 6 cut(s) 115, 389, 674, 696, 1067, 1073
HpyF10VI GCNNNNNNNGC 2 cut(s) 255, 798
HpyF3I CTNAG 2 cut(s) 201, 785
HpySE526I ACGT 1 cut(s) 139
Hsp92II CATG 3 cut(s) 182, 938, 1039
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 2 cut(s) 175, 211
KspAI GTTAAC 1 cut(s) 706
LguI GCTCTTC 1 cut(s) 891
LmnI GCTCC 5 cut(s) 239, 274, 492, 789, 880
Lsp1109I GCAGC 3 cut(s) 254, 870, 1053
LweI GCATC 1 cut(s) 772
MaeI CTAG 1 cut(s) 920
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 1 cut(s) 991
MflI RGATCY 3 cut(s) 6, 64, 833
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 4 cut(s) 289, 530, 603, 857
MlyI GAGTC 1 cut(s) 655
MmeI TCCRAC 1 cut(s) 811
MroI TCCGGA 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 2 cut(s) 533, 705
MslI CAYNNNNRTG 2 cut(s) 936, 957
MspI CCGG 3 cut(s) 10, 229, 270
MspR9I CCNGG 4 cut(s) 110, 229, 271, 367
MvaI CCWGG 2 cut(s) 110, 367
MwoI GCNNNNNNNGC 2 cut(s) 255, 798
NciI CCSGG 2 cut(s) 229, 271
NlaIII CATG 3 cut(s) 182, 938, 1039
NlaIV GGNNCC 4 cut(s) 8, 24, 186, 835
PciSI GCTCTTC 1 cut(s) 891
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 416
PflFI GACNNNGTC 1 cut(s) 847
PkrI GCNGC 3 cut(s) 244, 885, 1043
PleI GAGTC 1 cut(s) 654
PpsI GAGTC 1 cut(s) 654
PshAI GACNNNNGTC 1 cut(s) 740
Psp6I CCWGG 2 cut(s) 108, 365
PspGI CCWGG 2 cut(s) 108, 365
PspN4I GGNNCC 4 cut(s) 8, 24, 186, 835
PspOMI GGGCCC 1 cut(s) 184
PspPI GGNCC 3 cut(s) 184, 185, 550
PstI CTGCAG 1 cut(s) 1075
PstNI CAGNNNCTG 1 cut(s) 395
PsuI RGATCY 3 cut(s) 6, 64, 833
PsyI GACNNNGTC 1 cut(s) 847
RsaI GTAC 2 cut(s) 643, 749
RsaNI GTAC 2 cut(s) 642, 748
RseI CAYNNNNRTG 2 cut(s) 936, 957
SapI GCTCTTC 1 cut(s) 891
SaqAI TTAA 2 cut(s) 533, 705
SatI GCNGC 3 cut(s) 243, 884, 1042
Sau96I GGNCC 3 cut(s) 184, 185, 550
SchI GAGTC 1 cut(s) 655
ScrFI CCNGG 4 cut(s) 110, 229, 271, 367
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 1 cut(s) 772
SfcI CTRYAG 1 cut(s) 1071
SinI GGWCC 1 cut(s) 550
SmiMI CAYNNNNRTG 2 cut(s) 936, 957
Sse9I AATT 4 cut(s) 289, 530, 603, 857
SspMI CTAG 1 cut(s) 920
StyD4I CCNGG 4 cut(s) 108, 227, 269, 365
TaaI ACNGT 4 cut(s) 397, 631, 968, 1064
TaiI ACGT 1 cut(s) 142
TaqI TCGA 4 cut(s) 433, 621, 689, 700
TasI AATT 4 cut(s) 289, 530, 603, 857
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 2 cut(s) 533, 705
Tru9I TTAA 2 cut(s) 533, 705
TscAI CASTG 3 cut(s) 95, 1069, 1080
TseI GCWGC 3 cut(s) 242, 883, 1041
TspDTI ATGAA 2 cut(s) 975, 1022
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 3 cut(s) 95, 1069, 1080
Tth111I GACNNNGTC 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.