MD14G1055800.v1.1

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011978
Physical Location & Seq
Reverse (-)
5700667 .. 5702876
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1055800.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGAGGATCCGGAAACGACAGGTGCCCTTCCCTCTGTCGTCTCTCTCTCCGGTGCCTTTATCAGATCCCCTCCTCCACGACCTCAACTACTCACCACCACCCGTGGTGCAACTCCACCCCCAGCACCGCCATGATCCTCCAAATCCCTCCCAACCAGTTGAGAAAACTGTCCATGTGGGCCACCCCCCTCCTCAGCCCTCCGATCAGCGAGTCTTTCCGATCGGACGAGCAGGCAAGGGTTCTGATGGTTCTGGGGAGAATAAGAACAAAGAGAACAAGCAAGATTGTGGTTTGGAGATATCGATATTGAAAGGGGAAGAAGATGAGAGAGAGTCCAGAGAGGGAGGAGATCAGAAGGGAAATGATGATGATGATATCAGGGAGGAAAGCATATTGGTTGCAGAAACGTTAATGGGGTTTGCTTCGGGATCTAGTTCTTCTCACCCAGCTGCTGGGAGTTGGTTTAAAGGAGAGAAAGCGTTTCCATTGAAGAAGCGAAGAGGAAGCTTCGAGAGAGGAGCCAATAATATCGGACAGACGATAATGGATGATCAGAAAGACAAGAAATCAACGAAAACGAAGACGAACAAGAAAAGTGAACCTCGACAGCAACAAAATGACGATGTTCATCAGAAGCAAGACGATCATGTGATTGATGGCAATAGAGTCACTACTAGTGCTAGTGCAACAAGAAAGAGGGCAAGGGGAGGTGCACTCATGGAGGGGTCGCGGTGCAGTAGAGTTAACGGAAGAGGATGGAGGTGTTGCCAACAAACGCTTGTTGGGTACTCTCTTTGTGAGCATCACTTGGGGAAGGGTAGGTTAAGGAGCATGAATAGTGTCCGAAGCCGGTCAACGGCTGCGGCAACTGCTACTACCACTACTACTAGTACTAGAATTGCTTCTACTGAGACTGAATTCAAGCAACTATCAGCGTCTAGAATATTATCCGTGGAGTCATCACCGCCACAGAAAGAAGACACGAAAGCTGTTTGGCTGCTGGACGATGGTGATATTGGTGAAGGTAAAGGAAATGAAGGTGAAGATGAAGAGATCAAGAAGCCGTTGCTGGTTACAAAGAAGCGGATGAAGCTTGGGATCGTTAAAGCCCGATCCATGAGCAGCTTGCTAGGCCAAACGATTAGTCGTACAATGGCTGATGAGGATGATGATAATAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

395

Amino Acids

43.35

Weight (kDa)

9.03

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 241 - 281 1.3e-15 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 22, 52
AccB7I CCANNNNNTGG 1 cut(s) 452
AccII CGCG 1 cut(s) 730
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 5 cut(s) 127, 730, 863, 965, 1084
AclI AACGTT 1 cut(s) 407
AclWI GGATC 6 cut(s) 14, 59, 128, 436, 1106, 1107
AcsI RAATTY 1 cut(s) 917
AfaI GTAC 3 cut(s) 788, 892, 1150
AfiI CCNNNNNNNGG 2 cut(s) 452, 856
AgsI TTSAA 3 cut(s) 310, 490, 922
AhlI ACTAGT 2 cut(s) 674, 887
AluBI AGCT 5 cut(s) 449, 507, 989, 1093, 1125
AluI AGCT 5 cut(s) 449, 507, 989, 1093, 1125
Alw21I GWGCWC 1 cut(s) 715
Alw26I GTCTC 2 cut(s) 45, 905
Alw44I GTGCAC 1 cut(s) 711
AlwI GGATC 6 cut(s) 14, 59, 128, 436, 1106, 1107
AlwNI CAGNNNCTG 1 cut(s) 452
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 2 cut(s) 178, 1132
ApaLI GTGCAC 1 cut(s) 711
ApeKI GCWGC 4 cut(s) 449, 860, 997, 1122
ApoI RAATTY 1 cut(s) 917
ArsI GACNNNNNNTTYG 2 cut(s) 1129, 1161
Asp700I GAANNNNTTC 1 cut(s) 901
AspS9I GGNCC 1 cut(s) 178
AsuHPI GGTGA 6 cut(s) 84, 434, 954, 1022, 1031, 1052
BaeGI GKGCMC 2 cut(s) 27, 715
BamHI GGATCC 1 cut(s) 6
BanI GGYRCC 2 cut(s) 22, 52
BbsI GAAGAC 2 cut(s) 587, 984
Bbv12I GWGCWC 1 cut(s) 715
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 4 cut(s) 436, 847, 984, 1134
BccI CCATC 4 cut(s) 239, 650, 750, 1001
BceAI ACGGC 2 cut(s) 873, 1048
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 45, 905
BcuI ACTAGT 2 cut(s) 674, 887
BfaI CTAG 7 cut(s) 432, 675, 681, 888, 894, 939, 1130
BisI GCNGC 5 cut(s) 450, 861, 864, 998, 1123
BlsI GCNGC 5 cut(s) 451, 862, 865, 999, 1124
BmcAI AGTACT 1 cut(s) 892
BmgT120I GGNCC 1 cut(s) 178
BmiI GGNNCC 4 cut(s) 8, 24, 54, 520
BmsI GCATC 1 cut(s) 811
BpiI GAAGAC 2 cut(s) 587, 984
BplI GAGNNNNNCTC 2 cut(s) 699, 731
Bpu10I CCTNAGC 1 cut(s) 192
Bsa29I ATCGAT 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 627
BsaJI CCNNGG 2 cut(s) 102, 951
BsaWI WCCGGW 2 cut(s) 9, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 452, 856
Bse118I RCCGGY 1 cut(s) 849
Bse1I ACTGG 1 cut(s) 155
Bse8I GATNNNNATC 1 cut(s) 627
BseAI TCCGGA 1 cut(s) 9
BseCI ATCGAT 1 cut(s) 302
BseDI CCNNGG 2 cut(s) 102, 951
BseGI GGATG 4 cut(s) 553, 761, 1092, 1171
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 2 cut(s) 452, 856
BseMII CTCAG 2 cut(s) 206, 900
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 4 cut(s) 62, 180, 360, 531
BseSI GKGCMC 2 cut(s) 27, 715
BseXI GCAGC 4 cut(s) 436, 847, 984, 1134
BseYI CCCAGC 3 cut(s) 120, 445, 452
BsgI GTGCAG 1 cut(s) 754
Bsh1236I CGCG 1 cut(s) 730
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 180, 1134
BshNI GGYRCC 2 cut(s) 22, 52
BshVI ATCGAT 1 cut(s) 302
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 715
BsiSI CCGG 3 cut(s) 10, 50, 850
BslI CCNNNNNNNGG 2 cut(s) 452, 856
BsmAI GTCTC 2 cut(s) 45, 905
BsmBI CGTCTC 1 cut(s) 45
BsnI GGCC 2 cut(s) 180, 1134
Bsp1286I GDGCHC 2 cut(s) 27, 715
Bsp13I TCCGGA 1 cut(s) 9
BspACI CCGC 5 cut(s) 127, 730, 863, 965, 1084
BspANI GGCC 2 cut(s) 180, 1134
BspCNI CTCAG 2 cut(s) 205, 901
BspDI ATCGAT 1 cut(s) 302
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 1 cut(s) 730
BspLI GGNNCC 4 cut(s) 8, 24, 54, 520
BspPI GGATC 6 cut(s) 14, 59, 128, 436, 1106, 1107
BspT107I GGYRCC 2 cut(s) 22, 52
BsrFI RCCGGY 1 cut(s) 849
BsrI ACTGG 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 849
BssECI CCNNGG 2 cut(s) 102, 951
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 3 cut(s) 493, 745, 1044
BstC8I GCNNGC 2 cut(s) 232, 1127
BstDEI CTNAG 2 cut(s) 192, 909
BstDSI CCRYGG 2 cut(s) 102, 951
BstF5I GGATG 4 cut(s) 553, 761, 1092, 1171
BstFNI CGCG 1 cut(s) 730
BstMAI GTCTC 2 cut(s) 45, 905
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 3 cut(s) 869, 1090, 1131
BstSLI GKGCMC 2 cut(s) 27, 715
BstUI CGCG 1 cut(s) 730
BstV1I GCAGC 4 cut(s) 436, 847, 984, 1134
BstV2I GAAGAC 2 cut(s) 587, 984
BstX2I RGATCY 3 cut(s) 6, 64, 428
BstYI RGATCY 3 cut(s) 6, 64, 428
Bsu15I ATCGAT 1 cut(s) 302
BsuRI GGCC 2 cut(s) 180, 1134
BsuTUI ATCGAT 1 cut(s) 302
BtgI CCRYGG 2 cut(s) 102, 951
BtsCI GGATG 4 cut(s) 553, 761, 1092, 1171
Cac8I GCNNGC 2 cut(s) 232, 1127
CaiI CAGNNNCTG 1 cut(s) 452
Cfr10I RCCGGY 1 cut(s) 849
Cfr13I GGNCC 1 cut(s) 178
ClaI ATCGAT 1 cut(s) 302
CseI GACGC 1 cut(s) 924
Csp6I GTAC 3 cut(s) 787, 891, 1149
CviAII CATG 6 cut(s) 131, 173, 647, 718, 832, 1117
CviQI GTAC 3 cut(s) 787, 891, 1149
DdeI CTNAG 2 cut(s) 192, 909
DraI TTTAAA 1 cut(s) 466
Eam1104I CTCTTC 3 cut(s) 493, 745, 1044
EarI CTCTTC 3 cut(s) 493, 745, 1044
Eco32I GATATC 2 cut(s) 300, 376
EcoRI GAATTC 1 cut(s) 917
EcoRV GATATC 2 cut(s) 300, 376
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 6 cut(s) 134, 176, 650, 721, 835, 1120
FaiI YATR 7 cut(s) 132, 174, 392, 648, 719, 833, 1118
FatI CATG 6 cut(s) 130, 172, 646, 717, 831, 1116
FbaI TGATCA 1 cut(s) 550
Fnu4HI GCNGC 5 cut(s) 450, 861, 864, 998, 1123
FokI GGATG 4 cut(s) 560, 768, 1099, 1178
Fsp4HI GCNGC 5 cut(s) 450, 861, 864, 998, 1123
FspBI CTAG 7 cut(s) 432, 675, 681, 888, 894, 939, 1130
GluI GCNGC 5 cut(s) 450, 861, 864, 998, 1123
GsaI CCCAGC 3 cut(s) 124, 449, 456
HaeIII GGCC 2 cut(s) 180, 1134
HapII CCGG 3 cut(s) 10, 50, 850
HgaI GACGC 1 cut(s) 924
Hin1II CATG 6 cut(s) 134, 176, 650, 721, 835, 1120
HincII GTYRAC 2 cut(s) 745, 855
HindII GTYRAC 2 cut(s) 745, 855
HindIII AAGCTT 2 cut(s) 505, 1091
HinfI GANTC 4 cut(s) 210, 332, 666, 956
HpaI GTTAAC 1 cut(s) 745
HpaII CCGG 3 cut(s) 10, 50, 850
HphI GGTGA 6 cut(s) 84, 434, 954, 1022, 1031, 1052
Hpy166II GTNNAC 4 cut(s) 599, 713, 745, 855
Hpy188III TCNNGA 6 cut(s) 10, 336, 426, 511, 939, 1057
Hpy8I GTNNAC 4 cut(s) 599, 713, 745, 855
HpyAV CCTTC 5 cut(s) 37, 349, 808, 1016, 1031
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 5 cut(s) 109, 401, 686, 713, 735
HpyF10VI GCNNNNNNNGC 3 cut(s) 869, 1090, 1131
HpyF3I CTNAG 2 cut(s) 192, 909
HpySE526I ACGT 1 cut(s) 407
Hsp92II CATG 6 cut(s) 134, 176, 650, 721, 835, 1120
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 550
KspAI GTTAAC 1 cut(s) 745
LmnI GCTCC 2 cut(s) 518, 828
Lsp1109I GCAGC 4 cut(s) 436, 847, 984, 1134
LweI GCATC 1 cut(s) 811
MaeI CTAG 7 cut(s) 432, 675, 681, 888, 894, 939, 1130
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 2 cut(s) 667, 1072
MflI RGATCY 3 cut(s) 6, 64, 428
MhlI GDGCHC 2 cut(s) 27, 715
MluCI AATT 2 cut(s) 897, 917
MlyI GAGTC 4 cut(s) 219, 341, 675, 965
MroI TCCGGA 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 901
MseI TTAA 5 cut(s) 410, 465, 744, 824, 1104
MslI CAYNNNNRTG 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 3 cut(s) 10, 50, 850
MvnI CGCG 1 cut(s) 730
MwoI GCNNNNNNNGC 3 cut(s) 869, 1090, 1131
NlaIII CATG 6 cut(s) 134, 176, 650, 721, 835, 1120
NlaIV GGNNCC 4 cut(s) 8, 24, 54, 520
NmuCI GTSAC 1 cut(s) 667
PdmI GAANNNNTTC 1 cut(s) 901
PflMI CCANNNNNTGG 1 cut(s) 452
PkrI GCNGC 5 cut(s) 451, 862, 865, 999, 1124
Ple19I CGATCG 1 cut(s) 222
PleI GAGTC 4 cut(s) 218, 340, 674, 964
PpsI GAGTC 4 cut(s) 218, 340, 674, 964
Psp1406I AACGTT 1 cut(s) 407
PspFI CCCAGC 3 cut(s) 120, 445, 452
PspN4I GGNNCC 4 cut(s) 8, 24, 54, 520
PspPI GGNCC 1 cut(s) 178
PstNI CAGNNNCTG 1 cut(s) 452
PsuI RGATCY 3 cut(s) 6, 64, 428
PvuI CGATCG 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 3 cut(s) 788, 892, 1150
RsaNI GTAC 3 cut(s) 787, 891, 1149
RseI CAYNNNNRTG 1 cut(s) 129
SaqAI TTAA 5 cut(s) 410, 465, 744, 824, 1104
SatI GCNGC 5 cut(s) 450, 861, 864, 998, 1123
Sau96I GGNCC 1 cut(s) 178
ScaI AGTACT 1 cut(s) 892
SchI GAGTC 4 cut(s) 219, 341, 675, 965
SduI GDGCHC 2 cut(s) 27, 715
SfaNI GCATC 1 cut(s) 811
SmiMI CAYNNNNRTG 1 cut(s) 129
SpeI ACTAGT 2 cut(s) 674, 887
Sse9I AATT 2 cut(s) 897, 917
SsiI CCGC 5 cut(s) 127, 730, 863, 965, 1084
SspI AATATT 1 cut(s) 945
SspMI CTAG 7 cut(s) 432, 675, 681, 888, 894, 939, 1130
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 410
TaqI TCGA 3 cut(s) 302, 510, 604
TasI AATT 2 cut(s) 897, 917
TatI WGTACW 1 cut(s) 890
TauI GCSGC 1 cut(s) 866
Tru1I TTAA 5 cut(s) 410, 465, 744, 824, 1104
Tru9I TTAA 5 cut(s) 410, 465, 744, 824, 1104
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 4 cut(s) 449, 860, 997, 1122
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 5 cut(s) 617, 848, 1050, 1062, 1103
TspGWI ACGGA 2 cut(s) 762, 940
Van91I CCANNNNNTGG 1 cut(s) 452
VneI GTGCAC 1 cut(s) 711
XapI RAATTY 1 cut(s) 917
XbaI TCTAGA 1 cut(s) 938
XmnI GAANNNNTTC 1 cut(s) 901
XspI CTAG 7 cut(s) 432, 675, 681, 888, 894, 939, 1130
ZrmI AGTACT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.