Prupe.7G079000_v2.0.a1

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0011978
Physical Location & Seq
Reverse (-)
11471619 .. 11474421
2803 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G079000.1

Sequence Viewer

Length: 1218 bp
ATGAGGATCCGGAAAAGACAGGTGCCCTTCCCTCTCTCATCTCTCTCTCCGGTGCCTTTATCAGATCCCCTCCTCCACAACCTCAACTACTCACCACCATCACCACCACCCATGGTGCAACTCCACACCCACCATGATCCTGCCCCCACCCAACCAAAACCCTCCAACCCAGTTGAGAAAACCGCCCGGGTGGGCCATCCCCATCCTCAGCCGTCTGATCATCATCTCAATCAAGGTCTCTTGCCGATCGGACGGGCAGGGAAAGGCTGCGGTTTTTCTGATGCTGCTGGTGGGGATAAACACAAGGAGCACAACAAGCAAGATTGTGGTTTGGAGCTCTTGATATTGAAAGGGGAAGAAGAAGAAGATGAGAGAGGATCAAGAGATGGAGAGAATAATGATATCAGGAAGGAAAGCATATTGGGTGCAGAAACGTTGATGGGCTTTGCTTCAGGATCATCAAGTTTTTCTCACCGAGCCGTTGGGAGATGGTGTGAAGGAGAGAAAGCGTTCCCGTTGAAGAAGCGAAGAGGGAGCTTCGGAAGAGGAGCGAATATGGAGGAGAAAGACAGGAAATCAATGAAGACCAAGATGAACAAAAAATGTGCAACTCAACAGCAAAATGATGATGATCAGAAGCAAGAGGATGAGGAGGCCAAACAAGGCCTTGATCATGCCAATATTATTGTTCGTAGTAATTCGAGTGCAACAAAGAAAAGGGCAAGAGGCAGCGGTGCACTCATGGAGGGGTCGCGGTGCAGTCGAGTTAATGGAAGAGGATGGAGGTGTTGCCAGCAAACTCTTGTTGGCTACTCTCTTTGCGAGCATCACTTGGGCAAGGGAAGGTTGAGGAGCATGACCAGCGTCAGAAGCCGGTCTATGGCAACGACGACGACAAGTACTACTACTACTTCTACTGCAAGTACTGCGTCCATTGAGAAGGAGCTCAAGCGGTTATCAGCATCTAGATCTTCTGAGGAGTCGCCATGGAAAGAAGACACCAAAGCTGCTGTGCCTCTAGATGAACATGATGTTGATGAAGCTAAAGGTGATCGTGATGAAAATGGAGGTGAAGAGGAAGGGAAGCCATTGCTGGTTAAAAGGAAAAGGATGAAGCTTGGGATTGTTAAAGCGCGATCCATGAGCAGCTTGCTTGGCCAAACCAACAGTGCAATTGCTGCTGTGGCTGATGAGGATGAAGATAATAATAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

406

Amino Acids

44.44

Weight (kDa)

8.98

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42040
fragaria_vesca FvH4_6g22070
malus_domestica MD12G1056100.v1.1 MD14G1055800.v1.1
prunus_persica Prupe.7G079000_v2.0.a1 Prupe.7G079000_v2.0.a1
pyrus_communis pycom12g04900 pycom14g04590
rosa_chinensis RchiOBHm_Chr3g0479411
rosa_laevigata RLG00000023578
rosa_multiflora Rmu_sc0000107.1_g000002
rosa_roxburghii Rroxscaffold_6G00402300
rosa_rugosa Rorug03G0175000
rosa_samantha Rh3AG226200 Rh3BG258900 Rh3BG259000 Rh3CG255100 Rh3DG250600
rosa_wichuraiana Rw3G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 22, 52
AccII CGCG 2 cut(s) 754, 1135
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 5 cut(s) 183, 270, 732, 754, 952
AclI AACGTT 1 cut(s) 434
AclWI GGATC 6 cut(s) 14, 59, 131, 385, 463, 1131
AcoI YGGCCR 1 cut(s) 1156
AcuI CTGAAG 1 cut(s) 435
AfaI GTAC 2 cut(s) 901, 925
AfiI CCNNNNNNNGG 1 cut(s) 880
AgsI TTSAA 2 cut(s) 349, 520
AluBI AGCT 7 cut(s) 337, 537, 946, 1007, 1043, 1117, 1149
AluI AGCT 7 cut(s) 337, 537, 946, 1007, 1043, 1117, 1149
Alw21I GWGCWC 4 cut(s) 312, 339, 739, 948
Alw26I GTCTC 1 cut(s) 242
Alw44I GTGCAC 1 cut(s) 735
AlwI GGATC 6 cut(s) 14, 59, 131, 385, 463, 1131
Ama87I CYCGRG 1 cut(s) 186
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 4 cut(s) 193, 654, 664, 1156
ApaLI GTGCAC 1 cut(s) 735
ApeKI GCWGC 6 cut(s) 267, 284, 729, 1007, 1146, 1178
Asp700I GAANNNNTTC 1 cut(s) 509
AspLEI GCGC 1 cut(s) 1135
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 2 cut(s) 187, 188
AsuHPI GGTGA 5 cut(s) 84, 93, 464, 1061, 1082
AvaI CYCGRG 1 cut(s) 186
BaeGI GKGCMC 2 cut(s) 27, 739
BalI TGGCCA 1 cut(s) 1158
BamHI GGATCC 1 cut(s) 6
BanI GGYRCC 2 cut(s) 22, 52
BanII GRGCYC 2 cut(s) 339, 948
BbsI GAAGAC 2 cut(s) 590, 1002
Bbv12I GWGCWC 4 cut(s) 312, 339, 739, 948
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 6 cut(s) 254, 271, 741, 994, 1158, 1165
BccI CCATC 7 cut(s) 106, 204, 210, 380, 433, 483, 774
BceAI ACGGC 2 cut(s) 196, 464
BclI TGATCA 3 cut(s) 217, 631, 670
BcnI CCSGG 2 cut(s) 187, 188
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 2 cut(s) 966, 1019
BglII AGATCT 1 cut(s) 968
BisI GCNGC 6 cut(s) 268, 285, 730, 1008, 1147, 1179
BlsI GCNGC 6 cut(s) 269, 286, 731, 1009, 1148, 1180
BmcAI AGTACT 2 cut(s) 901, 925
Bme1390I CCNGG 2 cut(s) 187, 188
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 3 cut(s) 8, 24, 54
BmrFI CCNGG 2 cut(s) 187, 188
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 3 cut(s) 271, 835, 971
BmuI ACTGGG 1 cut(s) 164
BoxI GACNNNNGTC 1 cut(s) 863
BpiI GAAGAC 2 cut(s) 590, 1002
Bpu10I CCTNAGC 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 932
BpuMI CCSGG 2 cut(s) 187, 188
BsaBI GATNNNNATC 2 cut(s) 222, 630
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 3 cut(s) 111, 186, 986
BsaWI WCCGGW 2 cut(s) 9, 49
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 1 cut(s) 880
Bse118I RCCGGY 1 cut(s) 873
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 1088
Bse8I GATNNNNATC 2 cut(s) 222, 630
BseAI TCCGGA 1 cut(s) 9
BseDI CCNNGG 3 cut(s) 111, 186, 986
BseGI GGATG 6 cut(s) 196, 202, 652, 785, 1116, 1201
BseJI GATNNNNATC 2 cut(s) 222, 630
BseLI CCNNNNNNNGG 1 cut(s) 880
BseMI GCAATG 1 cut(s) 1088
BseMII CTCAG 2 cut(s) 221, 966
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 6 cut(s) 62, 561, 575, 665, 865, 992
BseSI GKGCMC 2 cut(s) 27, 739
BseXI GCAGC 6 cut(s) 254, 271, 741, 994, 1158, 1165
BsgI GTGCAG 2 cut(s) 447, 778
Bsh1236I CGCG 2 cut(s) 754, 1135
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 4 cut(s) 195, 656, 666, 1158
BshNI GGYRCC 2 cut(s) 22, 52
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 4 cut(s) 312, 339, 739, 948
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 4 cut(s) 10, 50, 187, 874
BslI CCNNNNNNNGG 1 cut(s) 880
BsmAI GTCTC 1 cut(s) 242
BsnI GGCC 4 cut(s) 195, 656, 666, 1158
Bso31I GGTCTC 1 cut(s) 242
BsoBI CYCGRG 1 cut(s) 186
Bsp1286I GDGCHC 5 cut(s) 27, 312, 339, 739, 948
Bsp13I TCCGGA 1 cut(s) 9
Bsp19I CCATGG 2 cut(s) 111, 986
BspACI CCGC 5 cut(s) 183, 270, 732, 754, 952
BspANI GGCC 4 cut(s) 195, 656, 666, 1158
BspCNI CTCAG 2 cut(s) 220, 967
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 2 cut(s) 754, 1135
BspLI GGNNCC 3 cut(s) 8, 24, 54
BspPI GGATC 6 cut(s) 14, 59, 131, 385, 463, 1131
BspT107I GGYRCC 2 cut(s) 22, 52
BspTNI GGTCTC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 1088
BsrFI RCCGGY 1 cut(s) 873
BsrI ACTGG 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 873
BssECI CCNNGG 3 cut(s) 111, 186, 986
BssT1I CCWWGG 2 cut(s) 111, 986
Bst4CI ACNGT 1 cut(s) 1169
Bst6I CTCTTC 4 cut(s) 523, 538, 769, 1068
BstAPI GCANNNNNTGC 2 cut(s) 926, 1178
BstC8I GCNNGC 3 cut(s) 794, 824, 1151
BstDEI CTNAG 2 cut(s) 207, 975
BstDSI CCRYGG 2 cut(s) 111, 986
BstF5I GGATG 6 cut(s) 196, 202, 652, 785, 1116, 1201
BstFNI CGCG 2 cut(s) 754, 1135
BstHHI GCGC 1 cut(s) 1135
BstMAI GTCTC 1 cut(s) 242
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 7 cut(s) 316, 861, 870, 926, 1155, 1178, 1184
BstPAI GACNNNNGTC 1 cut(s) 863
BstSCI CCNGG 2 cut(s) 185, 186
BstSLI GKGCMC 2 cut(s) 27, 739
BstUI CGCG 2 cut(s) 754, 1135
BstV1I GCAGC 6 cut(s) 254, 271, 741, 994, 1158, 1165
BstV2I GAAGAC 2 cut(s) 590, 1002
BstX2I RGATCY 3 cut(s) 6, 64, 968
BstYI RGATCY 3 cut(s) 6, 64, 968
BsuRI GGCC 4 cut(s) 195, 656, 666, 1158
BtgI CCRYGG 2 cut(s) 111, 986
BtsCI GGATG 6 cut(s) 196, 202, 652, 785, 1116, 1201
BtsIMutI CAGTG 1 cut(s) 1174
Cac8I GCNNGC 3 cut(s) 794, 824, 1151
CfoI GCGC 1 cut(s) 1135
Cfr10I RCCGGY 1 cut(s) 873
Cfr13I GGNCC 1 cut(s) 193
Cfr9I CCCGGG 1 cut(s) 186
CseI GACGC 2 cut(s) 853, 918
Csp6I GTAC 2 cut(s) 900, 924
CviAII CATG 8 cut(s) 112, 134, 674, 742, 856, 987, 1028, 1141
CviQI GTAC 2 cut(s) 900, 924
DdeI CTNAG 2 cut(s) 207, 975
EaeI YGGCCR 1 cut(s) 1156
Eam1104I CTCTTC 4 cut(s) 523, 538, 769, 1068
EarI CTCTTC 4 cut(s) 523, 538, 769, 1068
Ecl136II GAGCTC 2 cut(s) 337, 946
Eco130I CCWWGG 2 cut(s) 111, 986
Eco147I AGGCCT 1 cut(s) 666
Eco24I GRGCYC 2 cut(s) 339, 948
Eco31I GGTCTC 1 cut(s) 242
Eco32I GATATC 1 cut(s) 403
Eco53kI GAGCTC 2 cut(s) 337, 946
Eco57I CTGAAG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 186
EcoICRI GAGCTC 2 cut(s) 337, 946
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 2 cut(s) 111, 986
EcoT38I GRGCYC 2 cut(s) 339, 948
ErhI CCWWGG 2 cut(s) 111, 986
FaeI CATG 8 cut(s) 115, 137, 677, 745, 859, 990, 1031, 1144
FatI CATG 8 cut(s) 111, 133, 673, 741, 855, 986, 1027, 1140
FbaI TGATCA 3 cut(s) 217, 631, 670
Fnu4HI GCNGC 6 cut(s) 268, 285, 730, 1008, 1147, 1179
FokI GGATG 6 cut(s) 183, 189, 659, 792, 1123, 1208
FriOI GRGCYC 2 cut(s) 339, 948
Fsp4HI GCNGC 6 cut(s) 268, 285, 730, 1008, 1147, 1179
FspBI CTAG 2 cut(s) 966, 1019
GlaI GCGC 1 cut(s) 1134
GluI GCNGC 6 cut(s) 268, 285, 730, 1008, 1147, 1179
HaeIII GGCC 4 cut(s) 195, 656, 666, 1158
HapII CCGG 4 cut(s) 10, 50, 187, 874
HgaI GACGC 2 cut(s) 853, 918
HhaI GCGC 1 cut(s) 1135
Hin1II CATG 8 cut(s) 115, 137, 677, 745, 859, 990, 1031, 1144
Hin6I GCGC 1 cut(s) 1133
HinP1I GCGC 1 cut(s) 1133
HindIII AAGCTT 1 cut(s) 1115
HinfI GANTC 1 cut(s) 980
HpaII CCGG 4 cut(s) 10, 50, 187, 874
HphI GGTGA 5 cut(s) 84, 93, 464, 1061, 1082
Hpy166II GTNNAC 1 cut(s) 737
Hpy188I TCNGA 8 cut(s) 64, 217, 251, 280, 542, 636, 869, 976
Hpy188III TCNNGA 8 cut(s) 10, 340, 381, 406, 453, 966, 1019, 1055
Hpy8I GTNNAC 1 cut(s) 737
Hpy99I CGWCG 2 cut(s) 892, 895
HpyAV CCTTC 6 cut(s) 37, 403, 491, 837, 934, 1073
HpyCH4III ACNGT 1 cut(s) 1169
HpyCH4IV ACGT 1 cut(s) 434
HpyCH4V TGCA 8 cut(s) 118, 428, 608, 707, 737, 759, 920, 1172
HpyF10VI GCNNNNNNNGC 7 cut(s) 316, 861, 870, 926, 1155, 1178, 1184
HpyF3I CTNAG 2 cut(s) 207, 975
HpySE526I ACGT 1 cut(s) 434
Hsp92II CATG 8 cut(s) 115, 137, 677, 745, 859, 990, 1031, 1144
HspAI GCGC 1 cut(s) 1133
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 3 cut(s) 217, 631, 670
LmnI GCTCC 6 cut(s) 307, 334, 534, 548, 852, 943
Lsp1109I GCAGC 6 cut(s) 254, 271, 741, 994, 1158, 1165
LweI GCATC 3 cut(s) 271, 835, 971
MaeI CTAG 2 cut(s) 966, 1019
MaeII ACGT 1 cut(s) 434
MfeI CAATTG 1 cut(s) 1173
MflI RGATCY 3 cut(s) 6, 64, 968
MhlI GDGCHC 5 cut(s) 27, 312, 339, 739, 948
MlsI TGGCCA 1 cut(s) 1158
MluCI AATT 2 cut(s) 697, 1173
MluNI TGGCCA 1 cut(s) 1158
MlyI GAGTC 1 cut(s) 989
MmeI TCCRAC 1 cut(s) 189
Mox20I TGGCCA 1 cut(s) 1158
MroI TCCGGA 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 509
MscI TGGCCA 1 cut(s) 1158
MseI TTAA 3 cut(s) 768, 1098, 1128
Msp20I TGGCCA 1 cut(s) 1158
MspA1I CMGCKG 1 cut(s) 732
MspI CCGG 4 cut(s) 10, 50, 187, 874
MspR9I CCNGG 2 cut(s) 187, 188
MunI CAATTG 1 cut(s) 1173
MvnI CGCG 2 cut(s) 754, 1135
MwoI GCNNNNNNNGC 7 cut(s) 316, 861, 870, 926, 1155, 1178, 1184
NciI CCSGG 2 cut(s) 187, 188
NcoI CCATGG 2 cut(s) 111, 986
NlaIII CATG 8 cut(s) 115, 137, 677, 745, 859, 990, 1031, 1144
NlaIV GGNNCC 3 cut(s) 8, 24, 54
PceI AGGCCT 1 cut(s) 666
PdmI GAANNNNTTC 1 cut(s) 509
PkrI GCNGC 6 cut(s) 269, 286, 731, 1009, 1148, 1180
Ple19I CGATCG 1 cut(s) 249
PleI GAGTC 1 cut(s) 988
PpsI GAGTC 1 cut(s) 988
PshAI GACNNNNGTC 1 cut(s) 863
Psp124BI GAGCTC 2 cut(s) 339, 948
Psp1406I AACGTT 1 cut(s) 434
PspN4I GGNNCC 3 cut(s) 8, 24, 54
PspPI GGNCC 1 cut(s) 193
PsuI RGATCY 3 cut(s) 6, 64, 968
PvuI CGATCG 1 cut(s) 249
RsaI GTAC 2 cut(s) 901, 925
RsaNI GTAC 2 cut(s) 900, 924
SacI GAGCTC 2 cut(s) 339, 948
SaqAI TTAA 3 cut(s) 768, 1098, 1128
SatI GCNGC 6 cut(s) 268, 285, 730, 1008, 1147, 1179
Sau96I GGNCC 1 cut(s) 193
ScaI AGTACT 2 cut(s) 901, 925
SchI GAGTC 1 cut(s) 989
ScrFI CCNGG 2 cut(s) 187, 188
SduI GDGCHC 5 cut(s) 27, 312, 339, 739, 948
SfaNI GCATC 3 cut(s) 271, 835, 971
SmaI CCCGGG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 947
SmoI CTYRAG 1 cut(s) 947
Sse9I AATT 2 cut(s) 697, 1173
SseBI AGGCCT 1 cut(s) 666
SsiI CCGC 5 cut(s) 183, 270, 732, 754, 952
SspI AATATT 1 cut(s) 682
SspMI CTAG 2 cut(s) 966, 1019
SstI GAGCTC 2 cut(s) 339, 948
StuI AGGCCT 1 cut(s) 666
StyD4I CCNGG 2 cut(s) 185, 186
StyI CCWWGG 2 cut(s) 111, 986
TaaI ACNGT 1 cut(s) 1169
TaiI ACGT 1 cut(s) 437
TaqI TCGA 2 cut(s) 701, 763
TasI AATT 2 cut(s) 697, 1173
TatI WGTACW 2 cut(s) 899, 923
Tru1I TTAA 3 cut(s) 768, 1098, 1128
Tru9I TTAA 3 cut(s) 768, 1098, 1128
TscAI CASTG 1 cut(s) 1174
TseI GCWGC 6 cut(s) 267, 284, 729, 1007, 1146, 1178
TspDTI ATGAA 7 cut(s) 596, 608, 1038, 1053, 1074, 1127, 1212
TspMI CCCGGG 1 cut(s) 186
TspRI CASTG 1 cut(s) 1174
VneI GTGCAC 1 cut(s) 735
XbaI TCTAGA 2 cut(s) 965, 1018
XmaI CCCGGG 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 2 cut(s) 966, 1019
ZrmI AGTACT 2 cut(s) 901, 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.