FvH4_6g22410

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013027
Physical Location & Seq
Forward (+)
16109092 .. 16110134
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22410.t1

Sequence Viewer

Length: 510 bp
ATGCAGTCAATCAAGGAAGCCGCGGCCAATGTTTCTGCCTCAGCCAATTCTGGTATGGAGAAGACCAAGGCCACCATGCAGGAGAAGATGGAGCAGGTGAAGGCACATGACCCGATCCAAAAGGACATGGCAACTCAAAAGAAAGAAGCGCGGATTGCCGAGGCTGAGCAGCAGAAGCGTGAGGCGATGGATCACAACGCTGCCGCTAAACATGGAGCTACCCCGACTGGATCCACCGGTTACGGATCAGGACCGACGAACTACAGCACCGGCGGAGCAGGAACAGCCACATATTCAACCACCGGAGCTACTGGGCACCACACCGGGCTCAATCAGATGTCGGCACTGCCTGGACATGGGACCGGGGCGCCGACTGGCCAGGTGGTGGAGGGTGTTGCCAATGCGCATCCTATCGGAACAAACACCGGCACGGGGAGGACTACGGCACATAACCCCCATGTGGGTGGTAATGCCCCTGGGTCGTATGGGACTGGCGGGACATCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

170

Amino Acids

16.94

Weight (kDa)

8.06

Isoelectric Point (pI)

11.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 3.6e-19 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 85
Acc16I TGCGCA 1 cut(s) 405
Acc36I ACCTGC 1 cut(s) 85
AccB1I GGYRCC 2 cut(s) 315, 367
AccB7I CCANNNNNTGG 1 cut(s) 385
AccII CGCG 2 cut(s) 23, 151
AciI CCGC 6 cut(s) 21, 23, 151, 204, 273, 495
AclWI GGATC 5 cut(s) 109, 198, 225, 238, 253
AcoI YGGCCR 2 cut(s) 24, 376
AcyI GRCGYC 1 cut(s) 368
AfiI CCNNNNNNNGG 5 cut(s) 356, 385, 432, 460, 461
AgeI ACCGGT 1 cut(s) 236
AgsI TTSAA 1 cut(s) 297
AjnI CCWGG 3 cut(s) 349, 378, 475
AluBI AGCT 2 cut(s) 218, 308
AluI AGCT 2 cut(s) 218, 308
AlwI GGATC 5 cut(s) 109, 198, 225, 238, 253
AoxI GGCC 3 cut(s) 24, 69, 376
ApeKI GCWGC 2 cut(s) 169, 200
AsiGI ACCGGT 1 cut(s) 236
AspLEI GCGC 3 cut(s) 151, 370, 406
AspS9I GGNCC 2 cut(s) 251, 360
AsuC2I CCSGG 2 cut(s) 325, 364
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 2 cut(s) 251, 360
BaeGI GKGCMC 1 cut(s) 318
BalI TGGCCA 1 cut(s) 378
BamHI GGATCC 1 cut(s) 230
BanI GGYRCC 2 cut(s) 315, 367
BanII GRGCYC 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 68
BbvCI CCTCAGC 1 cut(s) 40
BbvI GCAGC 2 cut(s) 181, 187
BccI CCATC 2 cut(s) 82, 181
BceAI ACGGC 1 cut(s) 459
BciT130I CCWGG 3 cut(s) 351, 380, 477
BcnI CCSGG 2 cut(s) 325, 364
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 1 cut(s) 262
BfoI RGCGCY 1 cut(s) 371
BfuAI ACCTGC 1 cut(s) 85
BisI GCNGC 5 cut(s) 21, 24, 170, 201, 204
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 5 cut(s) 22, 25, 171, 202, 205
Bme1390I CCNGG 5 cut(s) 325, 351, 364, 380, 477
Bme18I GGWCC 2 cut(s) 251, 360
BmgT120I GGNCC 2 cut(s) 251, 360
BmiI GGNNCC 4 cut(s) 232, 317, 361, 369
BmrFI CCNGG 5 cut(s) 325, 351, 364, 380, 477
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 415
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 40
Bpu1102I GCTNAGC 1 cut(s) 165
BpuMI CCSGG 2 cut(s) 325, 364
BsaHI GRCGYC 1 cut(s) 368
BsaJI CCNNGG 6 cut(s) 21, 66, 159, 363, 475, 476
BsaWI WCCGGW 2 cut(s) 236, 302
Bsc4I CCNNNNNNNGG 5 cut(s) 356, 385, 432, 460, 461
Bse118I RCCGGY 3 cut(s) 236, 269, 425
Bse1I ACTGG 4 cut(s) 232, 316, 379, 496
BseBI CCWGG 3 cut(s) 351, 380, 477
BseDI CCNNGG 6 cut(s) 21, 66, 159, 363, 475, 476
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 5 cut(s) 356, 385, 432, 460, 461
BseMII CTCAG 2 cut(s) 54, 156
BseNI ACTGG 4 cut(s) 232, 316, 379, 496
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 2 cut(s) 181, 187
Bsh1236I CGCG 2 cut(s) 23, 151
BshFI GGCC 3 cut(s) 26, 71, 378
BshNI GGYRCC 2 cut(s) 315, 367
BshTI ACCGGT 1 cut(s) 236
BsiSI CCGG 6 cut(s) 237, 270, 303, 324, 363, 426
BslFI GGGAC 2 cut(s) 373, 502
BslI CCNNNNNNNGG 5 cut(s) 356, 385, 432, 460, 461
BsmFI GGGAC 2 cut(s) 373, 502
BsnI GGCC 3 cut(s) 26, 71, 378
Bsp1286I GDGCHC 2 cut(s) 318, 330
Bsp143I GATC 4 cut(s) 114, 190, 230, 245
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 6 cut(s) 21, 23, 151, 204, 273, 495
BspANI GGCC 3 cut(s) 26, 71, 378
BspCNI CTCAG 2 cut(s) 53, 157
BspFNI CGCG 2 cut(s) 23, 151
BspLI GGNNCC 4 cut(s) 232, 317, 361, 369
BspMI ACCTGC 1 cut(s) 85
BspPI GGATC 5 cut(s) 109, 198, 225, 238, 253
BspT107I GGYRCC 2 cut(s) 315, 367
BsrFI RCCGGY 3 cut(s) 236, 269, 425
BsrI ACTGG 4 cut(s) 232, 316, 379, 496
BssAI RCCGGY 3 cut(s) 236, 269, 425
BssECI CCNNGG 6 cut(s) 21, 66, 159, 363, 475, 476
BssMI GATC 4 cut(s) 114, 190, 230, 245
BssNI GRCGYC 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 3 cut(s) 351, 380, 477
BstACI GRCGYC 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 40, 165
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 2 cut(s) 23, 151
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 3 cut(s) 151, 370, 406
BstKTI GATC 4 cut(s) 117, 193, 233, 248
BstMBI GATC 4 cut(s) 114, 190, 230, 245
BstMWI GCNNNNNNNGC 3 cut(s) 155, 175, 284
BstNI CCWGG 3 cut(s) 351, 380, 477
BstSCI CCNGG 5 cut(s) 323, 349, 362, 378, 475
BstSFI CTRYAG 1 cut(s) 262
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 2 cut(s) 23, 151
BstV1I GCAGC 2 cut(s) 181, 187
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 3 cut(s) 26, 71, 378
BtgI CCRYGG 1 cut(s) 21
BtgZI GCGATG 1 cut(s) 200
BtsCI GGATG 1 cut(s) 406
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 1 cut(s) 344
BveI ACCTGC 1 cut(s) 85
CfoI GCGC 3 cut(s) 151, 370, 406
Cfr10I RCCGGY 3 cut(s) 236, 269, 425
Cfr13I GGNCC 2 cut(s) 251, 360
Cfr42I CCGCGG 1 cut(s) 24
CspAI ACCGGT 1 cut(s) 236
CviAII CATG 6 cut(s) 76, 107, 127, 212, 356, 458
DdeI CTNAG 2 cut(s) 40, 165
DinI GGCGCC 1 cut(s) 369
DpnI GATC 4 cut(s) 116, 192, 232, 247
DpnII GATC 4 cut(s) 114, 190, 230, 245
EaeI YGGCCR 2 cut(s) 24, 376
EciI GGCGGA 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 330
Eco47I GGWCC 2 cut(s) 251, 360
EcoRII CCWGG 3 cut(s) 349, 378, 475
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 330
EgeI GGCGCC 1 cut(s) 369
EheI GGCGCC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 6 cut(s) 79, 110, 130, 215, 359, 461
FaqI GGGAC 2 cut(s) 373, 502
FatI CATG 6 cut(s) 75, 106, 126, 211, 355, 457
FauI CCCGC 1 cut(s) 488
Fnu4HI GCNGC 5 cut(s) 21, 24, 170, 201, 204
FokI GGATG 1 cut(s) 393
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 5 cut(s) 21, 24, 170, 201, 204
FspAI RTGCGCAY 1 cut(s) 405
FspBI CTAG 1 cut(s) 504
FspI TGCGCA 1 cut(s) 405
GlaI GCGC 3 cut(s) 150, 369, 405
GluI GCNGC 5 cut(s) 21, 24, 170, 201, 204
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 3 cut(s) 26, 71, 378
HapII CCGG 6 cut(s) 237, 270, 303, 324, 363, 426
HhaI GCGC 3 cut(s) 151, 370, 406
Hin1I GRCGYC 1 cut(s) 368
Hin1II CATG 6 cut(s) 79, 110, 130, 215, 359, 461
Hin6I GCGC 3 cut(s) 149, 368, 404
HinP1I GCGC 3 cut(s) 149, 368, 404
HpaII CCGG 6 cut(s) 237, 270, 303, 324, 363, 426
HphI GGTGA 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 336, 416
Hpy188III TCNNGA 1 cut(s) 249
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 1 cut(s) 94
HpyCH4V TGCA 2 cut(s) 4, 79
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 175, 284
HpyF3I CTNAG 2 cut(s) 40, 165
Hsp92I GRCGYC 1 cut(s) 368
Hsp92II CATG 6 cut(s) 79, 110, 130, 215, 359, 461
HspAI GCGC 3 cut(s) 149, 368, 404
KasI GGCGCC 1 cut(s) 367
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 4 cut(s) 114, 190, 230, 245
LmnI GCTCC 4 cut(s) 91, 215, 275, 305
Lsp1109I GCAGC 2 cut(s) 181, 187
LweI GCATC 1 cut(s) 415
MaeI CTAG 1 cut(s) 504
MaeIII GTNAC 1 cut(s) 239
MalI GATC 4 cut(s) 116, 192, 232, 247
MboI GATC 4 cut(s) 114, 190, 230, 245
MboII GAAGA 2 cut(s) 73, 97
MflI RGATCY 1 cut(s) 230
MhlI GDGCHC 2 cut(s) 318, 330
MlsI TGGCCA 1 cut(s) 378
MluCI AATT 1 cut(s) 46
MluNI TGGCCA 1 cut(s) 378
Mly113I GGCGCC 1 cut(s) 368
MnlI CCTC 5 cut(s) 49, 154, 175, 382, 429
Mox20I TGGCCA 1 cut(s) 378
MscI TGGCCA 1 cut(s) 378
MslI CAYNNNNRTG 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 6 cut(s) 237, 270, 303, 324, 363, 426
MspR9I CCNGG 5 cut(s) 325, 351, 364, 380, 477
MvaI CCWGG 3 cut(s) 351, 380, 477
MvnI CGCG 2 cut(s) 23, 151
MwoI GCNNNNNNNGC 3 cut(s) 155, 175, 284
NarI GGCGCC 1 cut(s) 368
NciI CCSGG 2 cut(s) 325, 364
NdeII GATC 4 cut(s) 114, 190, 230, 245
NlaIII CATG 6 cut(s) 79, 110, 130, 215, 359, 461
NlaIV GGNNCC 4 cut(s) 232, 317, 361, 369
NmeAIII GCCGAG 1 cut(s) 184
NsbI TGCGCA 1 cut(s) 405
PaqCI CACCTGC 1 cut(s) 85
PasI CCCWGGG 1 cut(s) 476
PflMI CCANNNNNTGG 1 cut(s) 385
PinAI ACCGGT 1 cut(s) 236
PkrI GCNGC 5 cut(s) 22, 25, 171, 202, 205
PluTI GGCGCC 1 cut(s) 371
Psp6I CCWGG 3 cut(s) 349, 378, 475
PspGI CCWGG 3 cut(s) 349, 378, 475
PspN4I GGNNCC 4 cut(s) 232, 317, 361, 369
PspPI GGNCC 2 cut(s) 251, 360
PsuI RGATCY 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 462
SacII CCGCGG 1 cut(s) 24
SatI GCNGC 5 cut(s) 21, 24, 170, 201, 204
Sau3AI GATC 4 cut(s) 114, 190, 230, 245
Sau96I GGNCC 2 cut(s) 251, 360
ScrFI CCNGG 5 cut(s) 325, 351, 364, 380, 477
SduI GDGCHC 2 cut(s) 318, 330
SetI ASST 4 cut(s) 99, 220, 310, 384
SfaNI GCATC 1 cut(s) 415
SfcI CTRYAG 1 cut(s) 262
SfoI GGCGCC 1 cut(s) 369
Sfr303I CCGCGG 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 269
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 2 cut(s) 251, 360
SmiMI CAYNNNNRTG 1 cut(s) 462
Sse9I AATT 1 cut(s) 46
SsiI CCGC 6 cut(s) 21, 23, 151, 204, 273, 495
SspDI GGCGCC 1 cut(s) 367
SspMI CTAG 1 cut(s) 504
StyD4I CCNGG 5 cut(s) 323, 349, 362, 378, 475
StyI CCWWGG 1 cut(s) 66
TaqII GACCGA 1 cut(s) 268
TasI AATT 1 cut(s) 46
TauI GCSGC 3 cut(s) 23, 26, 206
TscAI CASTG 1 cut(s) 351
TseI GCWGC 2 cut(s) 169, 200
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 1 cut(s) 351
Van91I CCANNNNNTGG 1 cut(s) 385
VpaK11BI GGWCC 2 cut(s) 251, 360
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XspI CTAG 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.