Rh3DG255300

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0013027
Physical Location & Seq
Forward (+)
25250684 .. 25252980
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG255300.1

Sequence Viewer

Length: 507 bp
ATGCAGTCAATCAAGGAAGCCGCGGCCAACGTAGCTGCCTCTGCCAAGTCTGGCATGGAGAAGACCAAGGCCACCATGCAGGAGAAGATGGAGAAGATGAAGGCGCAGGACCCGATCCAAAAGGACATGGCAACTCAAAAGAAAGAAGGGCGGATTGCCGAGGCTGAGCAGCACAAGCGTGAGGCCATGGATCACAACACAGCCGCTAAGCATGGAGCAACTCCAGCTGGATCCACAGACTACGGCACTGGGCCGACAGGATACAGCACCAGCGGGGCTGGAACAGCCACATATCCAACCACCGGGGCTTCTGGGCAGCAAATCGGGTTCAATCAGATGTCTGCACTGCCCAGACATGGGACCGGGGAACCCGCTGGCCAGGTGGTTGAGGGTGTTGTCACCGCACATCCGATCGGAACCAACACCGGCACCGGGAGGACTACGGACCATAACACCCATGTGGGTGGTAATGCCCCTGGCTATGGGACTGGCGGGACTTATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

168

Amino Acids

17.21

Weight (kDa)

7.99

Isoelectric Point (pI)

19.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 7.9e-20 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 428
AccII CGCG 1 cut(s) 23
AciI CCGC 8 cut(s) 21, 23, 151, 204, 273, 372, 402, 492
AclWI GGATC 4 cut(s) 109, 198, 225, 238
AcoI YGGCCR 2 cut(s) 24, 376
AfiI CCNNNNNNNGG 5 cut(s) 302, 356, 357, 432, 482
AgsI TTSAA 1 cut(s) 331
AjnI CCWGG 2 cut(s) 378, 475
AleI CACNNNNGTG 2 cut(s) 177, 458
AluBI AGCT 2 cut(s) 35, 227
AluI AGCT 2 cut(s) 35, 227
AlwI GGATC 4 cut(s) 109, 198, 225, 238
AoxI GGCC 5 cut(s) 24, 69, 183, 251, 376
ApeKI GCWGC 3 cut(s) 35, 169, 316
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 4 cut(s) 109, 251, 360, 445
AsuC2I CCSGG 3 cut(s) 304, 364, 433
AsuHPI GGTGA 1 cut(s) 391
AvaII GGWCC 3 cut(s) 109, 360, 445
BalI TGGCCA 1 cut(s) 378
BamHI GGATCC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 22, 181, 328
BccI CCATC 1 cut(s) 82
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 2 cut(s) 380, 477
BciVI GTATCC 1 cut(s) 254
BcnI CCSGG 3 cut(s) 304, 364, 433
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 6 cut(s) 21, 24, 36, 170, 204, 317
BlpI GCTNAGC 2 cut(s) 165, 207
BlsI GCNGC 6 cut(s) 22, 25, 37, 171, 205, 318
Bme1390I CCNGG 5 cut(s) 304, 364, 380, 433, 477
Bme18I GGWCC 3 cut(s) 109, 360, 445
BmgT120I GGNCC 4 cut(s) 109, 251, 360, 445
BmiI GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
BmrFI CCNGG 5 cut(s) 304, 364, 380, 433, 477
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 207
Bpu1102I GCTNAGC 2 cut(s) 165, 207
BpuMI CCSGG 3 cut(s) 304, 364, 433
BsaJI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
Bsc4I CCNNNNNNNGG 5 cut(s) 302, 356, 357, 432, 482
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 2 cut(s) 253, 493
BseBI CCWGG 2 cut(s) 380, 477
BseDI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 5 cut(s) 302, 356, 357, 432, 482
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 253, 493
BseXI GCAGC 3 cut(s) 22, 181, 328
BsgI GTGCAG 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 414
BshFI GGCC 5 cut(s) 26, 71, 185, 253, 378
BshNI GGYRCC 1 cut(s) 428
BsiEI CGRYCG 1 cut(s) 414
BsiSI CCGG 4 cut(s) 303, 363, 426, 432
BslFI GGGAC 2 cut(s) 373, 499
BslI CCNNNNNNNGG 5 cut(s) 302, 356, 357, 432, 482
BsmFI GGGAC 2 cut(s) 373, 499
BsnI GGCC 5 cut(s) 26, 71, 185, 253, 378
Bsp143I GATC 4 cut(s) 114, 190, 230, 411
Bsp1720I GCTNAGC 2 cut(s) 165, 207
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 8 cut(s) 21, 23, 151, 204, 273, 372, 402, 492
BspANI GGCC 5 cut(s) 26, 71, 185, 253, 378
BspCNI CTCAG 1 cut(s) 157
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
BspPI GGATC 4 cut(s) 109, 198, 225, 238
BspT107I GGYRCC 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 2 cut(s) 253, 493
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
BssMI GATC 4 cut(s) 114, 190, 230, 411
BssT1I CCWWGG 2 cut(s) 66, 186
Bst2UI CCWGG 2 cut(s) 380, 477
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 2 cut(s) 165, 207
BstDSI CCRYGG 2 cut(s) 21, 186
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 106
BstKTI GATC 4 cut(s) 117, 193, 233, 414
BstMBI GATC 4 cut(s) 114, 190, 230, 411
BstMCI CGRYCG 1 cut(s) 414
BstMWI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 284
BstNI CCWGG 2 cut(s) 380, 477
BstSCI CCNGG 5 cut(s) 302, 362, 378, 431, 475
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 22, 181, 328
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 1 cut(s) 230
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 5 cut(s) 26, 71, 185, 253, 378
BtgI CCRYGG 2 cut(s) 21, 186
BtsCI GGATG 1 cut(s) 406
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 2 cut(s) 246, 344
Cac8I GCNNGC 1 cut(s) 376
CfoI GCGC 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 4 cut(s) 109, 251, 360, 445
Cfr42I CCGCGG 1 cut(s) 24
CviAII CATG 7 cut(s) 55, 76, 127, 187, 212, 356, 458
DdeI CTNAG 2 cut(s) 165, 207
DpnI GATC 4 cut(s) 116, 192, 232, 413
DpnII GATC 4 cut(s) 114, 190, 230, 411
EaeI YGGCCR 2 cut(s) 24, 376
EciI GGCGGA 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 66, 186
Eco47I GGWCC 3 cut(s) 109, 360, 445
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 2 cut(s) 378, 475
EcoT14I CCWWGG 2 cut(s) 66, 186
ErhI CCWWGG 2 cut(s) 66, 186
FaeI CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
FaqI GGGAC 2 cut(s) 373, 499
FatI CATG 7 cut(s) 54, 75, 126, 186, 211, 355, 457
FauI CCCGC 3 cut(s) 266, 379, 485
Fnu4HI GCNGC 6 cut(s) 21, 24, 36, 170, 204, 317
FokI GGATG 1 cut(s) 393
Fsp4HI GCNGC 6 cut(s) 21, 24, 36, 170, 204, 317
GlaI GCGC 1 cut(s) 105
GluI GCNGC 6 cut(s) 21, 24, 36, 170, 204, 317
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 5 cut(s) 26, 71, 185, 253, 378
HapII CCGG 4 cut(s) 303, 363, 426, 432
HhaI GCGC 1 cut(s) 106
Hin1II CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HpaII CCGG 4 cut(s) 303, 363, 426, 432
HphI GGTGA 1 cut(s) 391
Hpy188I TCNGA 3 cut(s) 336, 411, 416
HpyAV CCTTC 2 cut(s) 94, 140
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 3 cut(s) 4, 79, 344
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 284
HpyF3I CTNAG 2 cut(s) 165, 207
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
HspAI GCGC 1 cut(s) 104
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 4 cut(s) 114, 190, 230, 411
LmnI GCTCC 1 cut(s) 215
Lsp1109I GCAGC 3 cut(s) 22, 181, 328
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 397
MalI GATC 4 cut(s) 116, 192, 232, 413
MboI GATC 4 cut(s) 114, 190, 230, 411
MboII GAAGA 3 cut(s) 73, 97, 106
MflI RGATCY 1 cut(s) 230
MlsI TGGCCA 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 378
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 5 cut(s) 49, 154, 175, 382, 429
Mox20I TGGCCA 1 cut(s) 378
MscI TGGCCA 1 cut(s) 378
MslI CAYNNNNRTG 3 cut(s) 177, 458, 462
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 4 cut(s) 23, 227, 273, 374
MspI CCGG 4 cut(s) 303, 363, 426, 432
MspR9I CCNGG 5 cut(s) 304, 364, 380, 433, 477
MvaI CCWGG 2 cut(s) 380, 477
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 284
NciI CCSGG 3 cut(s) 304, 364, 433
NcoI CCATGG 1 cut(s) 186
NdeII GATC 4 cut(s) 114, 190, 230, 411
NlaIII CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
NlaIV GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 397
OliI CACNNNNGTG 2 cut(s) 177, 458
PkrI GCNGC 6 cut(s) 22, 25, 37, 171, 205, 318
Ple19I CGATCG 1 cut(s) 414
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 2 cut(s) 378, 475
PspGI CCWGG 2 cut(s) 378, 475
PspN4I GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
PspPI GGNCC 4 cut(s) 109, 251, 360, 445
PspPPI RGGWCCY 1 cut(s) 109
PsuI RGATCY 1 cut(s) 230
PvuI CGATCG 1 cut(s) 414
PvuII CAGCTG 1 cut(s) 227
RseI CAYNNNNRTG 3 cut(s) 177, 458, 462
SacII CCGCGG 1 cut(s) 24
SatI GCNGC 6 cut(s) 21, 24, 36, 170, 204, 317
Sau3AI GATC 4 cut(s) 114, 190, 230, 411
Sau96I GGNCC 4 cut(s) 109, 251, 360, 445
ScrFI CCNGG 5 cut(s) 304, 364, 380, 433, 477
SetI ASST 4 cut(s) 33, 37, 229, 384
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 3 cut(s) 109, 360, 445
SmiMI CAYNNNNRTG 3 cut(s) 177, 458, 462
SsiI CCGC 8 cut(s) 21, 23, 151, 204, 273, 372, 402, 492
StyD4I CCNGG 5 cut(s) 302, 362, 378, 431, 475
StyI CCWWGG 2 cut(s) 66, 186
TaiI ACGT 1 cut(s) 33
TauI GCSGC 3 cut(s) 23, 26, 206
TscAI CASTG 2 cut(s) 253, 351
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 3 cut(s) 35, 169, 316
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 113
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 2 cut(s) 253, 351
VpaK11BI GGWCC 3 cut(s) 109, 360, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.