Rh3BG262300

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0013027
Physical Location & Seq
Forward (+)
25408358 .. 25409585
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG262300.1

Sequence Viewer

Length: 579 bp
ATGGTTGATCGTACAGTTTTCTTTTTCAGACAAGTTTTGTTTTTCCAGCTAAAGAAAAAGAAGAAAGTGAAAATGCAGTCAATCAAGGAAGCCGCGGCCAACGTAGCTGCCTCTGCCAAGTCTGGTATGGAGAAGACCAAGGCCACCATGCAGGAGAAGATGGAGAAGATGAAGGCGCAGGACCCGATCCAAAAGGACATGGCAACTCAAAAGAAACAAGGGCGGATTGCCGAGGCTGAGCAGCACAAGCGTGAGGCCATGGATCACAACACCGCCGCTAAACATGGAACAGCTCCGGCTGGATCCACAGGCTACAGTACTGAGCCGACGGGATACAGCACCAACGGGGATGGAACAGCCACATATCCAACCACTGGGGCTACTGGGCAGCAAACCGGGTTCAATCAGATGTCCGCGCTGCCCGGACATGGGACCGGGGAACCCCCTGGCCAGGTGGTTGAGGGTGTTGCCACCGCACATCCGATCGGAACCAGCACCGGCACCGGGAGGACTACGGCCCATAACACCCATGTGGGTGGTAATGCCCCCGGCTATGGGACTGGCGGGACTTATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

192

Amino Acids

20.19

Weight (kDa)

9.64

Isoelectric Point (pI)

21.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 25 - 94 1.2e-19 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 500
AccB7I CCANNNNNTGG 1 cut(s) 374
AccII CGCG 2 cut(s) 95, 416
AciI CCGC 8 cut(s) 93, 95, 223, 273, 276, 414, 474, 564
AclWI GGATC 4 cut(s) 181, 270, 297, 310
AcoI YGGCCR 2 cut(s) 96, 448
AfaI GTAC 2 cut(s) 13, 319
AfiI CCNNNNNNNGG 7 cut(s) 374, 428, 429, 451, 504, 554, 555
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 2 cut(s) 445, 450
AleI CACNNNNGTG 2 cut(s) 249, 530
AluBI AGCT 3 cut(s) 49, 107, 293
AluI AGCT 3 cut(s) 49, 107, 293
AlwI GGATC 4 cut(s) 181, 270, 297, 310
AoxI GGCC 5 cut(s) 96, 141, 255, 448, 516
ApeKI GCWGC 4 cut(s) 107, 241, 388, 418
AspLEI GCGC 2 cut(s) 178, 418
AspS9I GGNCC 3 cut(s) 181, 432, 517
AsuC2I CCSGG 5 cut(s) 397, 423, 436, 505, 549
AvaII GGWCC 2 cut(s) 181, 432
BalI TGGCCA 1 cut(s) 450
BamHI GGATCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 140
BbvI GCAGC 4 cut(s) 94, 253, 400, 405
BccI CCATC 2 cut(s) 154, 344
BceAI ACGGC 1 cut(s) 531
BciT130I CCWGG 2 cut(s) 447, 452
BciVI GTATCC 1 cut(s) 326
BcnI CCSGG 5 cut(s) 397, 423, 436, 505, 549
BfmI CTRYAG 1 cut(s) 313
BfuI GTATCC 1 cut(s) 326
BisI GCNGC 7 cut(s) 93, 96, 108, 242, 276, 389, 419
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 7 cut(s) 94, 97, 109, 243, 277, 390, 420
BmcAI AGTACT 1 cut(s) 319
Bme1390I CCNGG 7 cut(s) 397, 423, 436, 447, 452, 505, 549
Bme18I GGWCC 2 cut(s) 181, 432
BmgT120I GGNCC 3 cut(s) 181, 432, 517
BmiI GGNNCC 6 cut(s) 183, 304, 433, 441, 490, 502
BmrFI CCNGG 7 cut(s) 397, 423, 436, 447, 452, 505, 549
BmrI ACTGGG 2 cut(s) 384, 393
BmuI ACTGGG 2 cut(s) 384, 393
BpiI GAAGAC 1 cut(s) 140
Bpu1102I GCTNAGC 1 cut(s) 237
BpuMI CCSGG 5 cut(s) 397, 423, 436, 505, 549
BsaJI CCNNGG 7 cut(s) 93, 138, 231, 258, 435, 445, 547
Bsc4I CCNNNNNNNGG 7 cut(s) 374, 428, 429, 451, 504, 554, 555
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 3 cut(s) 379, 388, 565
BseBI CCWGG 2 cut(s) 447, 452
BseDI CCNNGG 7 cut(s) 93, 138, 231, 258, 435, 445, 547
BseGI GGATG 2 cut(s) 355, 478
BseLI CCNNNNNNNGG 7 cut(s) 374, 428, 429, 451, 504, 554, 555
BseMII CTCAG 2 cut(s) 228, 312
BseNI ACTGG 3 cut(s) 379, 388, 565
BseXI GCAGC 4 cut(s) 94, 253, 400, 405
Bsh1236I CGCG 2 cut(s) 95, 416
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 5 cut(s) 98, 143, 257, 450, 518
BshNI GGYRCC 1 cut(s) 500
BsiEI CGRYCG 1 cut(s) 486
BsiSI CCGG 7 cut(s) 296, 396, 423, 435, 498, 504, 549
BslFI GGGAC 2 cut(s) 445, 571
BslI CCNNNNNNNGG 7 cut(s) 374, 428, 429, 451, 504, 554, 555
BsmFI GGGAC 2 cut(s) 445, 571
BsnI GGCC 5 cut(s) 98, 143, 257, 450, 518
Bsp143I GATC 5 cut(s) 7, 186, 262, 302, 483
Bsp1720I GCTNAGC 1 cut(s) 237
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 8 cut(s) 93, 95, 223, 273, 276, 414, 474, 564
BspANI GGCC 5 cut(s) 98, 143, 257, 450, 518
BspCNI CTCAG 2 cut(s) 229, 313
BspFNI CGCG 2 cut(s) 95, 416
BspLI GGNNCC 6 cut(s) 183, 304, 433, 441, 490, 502
BspPI GGATC 4 cut(s) 181, 270, 297, 310
BspT107I GGYRCC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 3 cut(s) 379, 388, 565
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 7 cut(s) 93, 138, 231, 258, 435, 445, 547
BssMI GATC 5 cut(s) 7, 186, 262, 302, 483
BssT1I CCWWGG 2 cut(s) 138, 258
Bst2UI CCWGG 2 cut(s) 447, 452
Bst4CI ACNGT 2 cut(s) 16, 317
BstDEI CTNAG 2 cut(s) 237, 321
BstDSI CCRYGG 2 cut(s) 93, 258
BstF5I GGATG 2 cut(s) 355, 478
BstFNI CGCG 2 cut(s) 95, 416
BstHHI GCGC 2 cut(s) 178, 418
BstKTI GATC 5 cut(s) 10, 189, 265, 305, 486
BstMBI GATC 5 cut(s) 7, 186, 262, 302, 483
BstMCI CGRYCG 1 cut(s) 486
BstMWI GCNNNNNNNGC 3 cut(s) 104, 113, 247
BstNI CCWGG 2 cut(s) 447, 452
BstSCI CCNGG 7 cut(s) 395, 421, 434, 445, 450, 503, 547
BstSFI CTRYAG 1 cut(s) 313
BstUI CGCG 2 cut(s) 95, 416
BstV1I GCAGC 4 cut(s) 94, 253, 400, 405
BstV2I GAAGAC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 302
BstXI CCANNNNNNTGG 1 cut(s) 536
BstYI RGATCY 1 cut(s) 302
BsuI GTATCC 1 cut(s) 326
BsuRI GGCC 5 cut(s) 98, 143, 257, 450, 518
BtgI CCRYGG 2 cut(s) 93, 258
BtsCI GGATG 2 cut(s) 355, 478
BtsIMutI CAGTG 1 cut(s) 372
CfoI GCGC 2 cut(s) 178, 418
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 3 cut(s) 181, 432, 517
Cfr42I CCGCGG 1 cut(s) 96
Csp6I GTAC 2 cut(s) 12, 318
CviAII CATG 6 cut(s) 148, 199, 259, 284, 428, 530
CviQI GTAC 2 cut(s) 12, 318
DdeI CTNAG 2 cut(s) 237, 321
DpnI GATC 5 cut(s) 9, 188, 264, 304, 485
DpnII GATC 5 cut(s) 7, 186, 262, 302, 483
EaeI YGGCCR 2 cut(s) 96, 448
EciI GGCGGA 1 cut(s) 238
Eco130I CCWWGG 2 cut(s) 138, 258
Eco47I GGWCC 2 cut(s) 181, 432
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 2 cut(s) 445, 450
EcoT14I CCWWGG 2 cut(s) 138, 258
ErhI CCWWGG 2 cut(s) 138, 258
FaeI CATG 6 cut(s) 151, 202, 262, 287, 431, 533
FaqI GGGAC 2 cut(s) 445, 571
FatI CATG 6 cut(s) 147, 198, 258, 283, 427, 529
FauI CCCGC 1 cut(s) 557
Fnu4HI GCNGC 7 cut(s) 93, 96, 108, 242, 276, 389, 419
FokI GGATG 2 cut(s) 362, 465
Fsp4HI GCNGC 7 cut(s) 93, 96, 108, 242, 276, 389, 419
GlaI GCGC 2 cut(s) 177, 417
GluI GCNGC 7 cut(s) 93, 96, 108, 242, 276, 389, 419
HaeIII GGCC 5 cut(s) 98, 143, 257, 450, 518
HapII CCGG 7 cut(s) 296, 396, 423, 435, 498, 504, 549
HhaI GCGC 2 cut(s) 178, 418
Hin1II CATG 6 cut(s) 151, 202, 262, 287, 431, 533
Hin6I GCGC 2 cut(s) 176, 416
HinP1I GCGC 2 cut(s) 176, 416
HpaII CCGG 7 cut(s) 296, 396, 423, 435, 498, 504, 549
Hpy188I TCNGA 4 cut(s) 29, 408, 483, 488
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 1 cut(s) 166
HpyCH4III ACNGT 2 cut(s) 16, 317
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 2 cut(s) 76, 151
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 113, 247
HpyF3I CTNAG 2 cut(s) 237, 321
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 6 cut(s) 151, 202, 262, 287, 431, 533
HspAI GCGC 2 cut(s) 176, 416
KspI CCGCGG 1 cut(s) 96
Kzo9I GATC 5 cut(s) 7, 186, 262, 302, 483
LmnI GCTCC 1 cut(s) 298
Lsp1109I GCAGC 4 cut(s) 94, 253, 400, 405
MaeII ACGT 1 cut(s) 102
MalI GATC 5 cut(s) 9, 188, 264, 304, 485
MboI GATC 5 cut(s) 7, 186, 262, 302, 483
MboII GAAGA 4 cut(s) 73, 145, 169, 178
MflI RGATCY 1 cut(s) 302
MlsI TGGCCA 1 cut(s) 450
MluNI TGGCCA 1 cut(s) 450
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 5 cut(s) 121, 226, 247, 454, 501
Mox20I TGGCCA 1 cut(s) 450
MscI TGGCCA 1 cut(s) 450
MslI CAYNNNNRTG 3 cut(s) 249, 530, 534
Msp20I TGGCCA 1 cut(s) 450
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 7 cut(s) 296, 396, 423, 435, 498, 504, 549
MspR9I CCNGG 7 cut(s) 397, 423, 436, 447, 452, 505, 549
MvaI CCWGG 2 cut(s) 447, 452
MvnI CGCG 2 cut(s) 95, 416
MwoI GCNNNNNNNGC 3 cut(s) 104, 113, 247
NciI CCSGG 5 cut(s) 397, 423, 436, 505, 549
NcoI CCATGG 1 cut(s) 258
NdeII GATC 5 cut(s) 7, 186, 262, 302, 483
NlaIII CATG 6 cut(s) 151, 202, 262, 287, 431, 533
NlaIV GGNNCC 6 cut(s) 183, 304, 433, 441, 490, 502
NmeAIII GCCGAG 1 cut(s) 256
OliI CACNNNNGTG 2 cut(s) 249, 530
PflMI CCANNNNNTGG 1 cut(s) 374
PkrI GCNGC 7 cut(s) 94, 97, 109, 243, 277, 390, 420
Ple19I CGATCG 1 cut(s) 486
PpuMI RGGWCCY 1 cut(s) 181
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 445, 450
PspGI CCWGG 2 cut(s) 445, 450
PspN4I GGNNCC 6 cut(s) 183, 304, 433, 441, 490, 502
PspPI GGNCC 3 cut(s) 181, 432, 517
PspPPI RGGWCCY 1 cut(s) 181
PsuI RGATCY 1 cut(s) 302
PvuI CGATCG 1 cut(s) 486
RsaI GTAC 2 cut(s) 13, 319
RsaNI GTAC 2 cut(s) 12, 318
RseI CAYNNNNRTG 3 cut(s) 249, 530, 534
SacII CCGCGG 1 cut(s) 96
SatI GCNGC 7 cut(s) 93, 96, 108, 242, 276, 389, 419
Sau3AI GATC 5 cut(s) 7, 186, 262, 302, 483
Sau96I GGNCC 3 cut(s) 181, 432, 517
ScaI AGTACT 1 cut(s) 319
ScrFI CCNGG 7 cut(s) 397, 423, 436, 447, 452, 505, 549
SetI ASST 5 cut(s) 51, 105, 109, 295, 456
SfcI CTRYAG 1 cut(s) 313
Sfr303I CCGCGG 1 cut(s) 96
SgrBI CCGCGG 1 cut(s) 96
SinI GGWCC 2 cut(s) 181, 432
SmiMI CAYNNNNRTG 3 cut(s) 249, 530, 534
SsiI CCGC 8 cut(s) 93, 95, 223, 273, 276, 414, 474, 564
StyD4I CCNGG 7 cut(s) 395, 421, 434, 445, 450, 503, 547
StyI CCWWGG 2 cut(s) 138, 258
TaaI ACNGT 2 cut(s) 16, 317
TaiI ACGT 1 cut(s) 105
TatI WGTACW 1 cut(s) 317
TauI GCSGC 3 cut(s) 95, 98, 278
TscAI CASTG 1 cut(s) 379
TseI GCWGC 4 cut(s) 107, 241, 388, 418
TspDTI ATGAA 1 cut(s) 185
TspRI CASTG 1 cut(s) 379
Van91I CCANNNNNTGG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 181, 432
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.