RLG00000023541

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013027
Physical Location & Seq
Reverse (-)
25073755 .. 25075282
1528 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023541

Sequence Viewer

Length: 576 bp
ATGCAGTCAATCAAGGAAGCCGCGGCCAACGTAGCTGCCTCTGCCAAGTCTGGCATGGAGAAGACCAAGGCCACCATGCAGGAGAAGATGGAGAAGATGAAGGCGCAGGACCCGATCCAAAAGGACATGGCAACTCAAAAGAAAGAAGGGCGGATTGCCGAGGCTGAGCAGCACAAGCGTGAGGCCATGGATCACAACACCGCCGCTAAACATGAAGCAACTCCGGCTGGATCCACAGGCTACGGCACTGGGCCGACGGGATACAGCAGCAGCGGGGCTGGAACAGCCACATATTCAACCACCGGGGCTACTGGGCCGCAAACCGGGTTCAATCAGATGTCCGCGCTGCCCGGACATGGGACCGAGGAACCCGCTGGCAAGGTGGTTGAGGGTATTGCCACCGCACATCCGATCGGAACCAACACCGGCACCGGGAGGACTACGGCCCATAACACCCATGTGGGTGTCCGACCCGCCGAATCCCTCGTCGATCGTTTGCAACCCTCTCACGCACCACTTCCGTCCCTTGCCTTAGCTCGGCTTCACCTAGTCAAGGCACGACTCTGTTCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

192

Amino Acids

19.8

Weight (kDa)

9.1

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 1.1e-19 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 428
AccII CGCG 2 cut(s) 23, 344
AclWI GGATC 4 cut(s) 109, 198, 225, 238
AcoI YGGCCR 1 cut(s) 24
AfiI CCNNNNNNNGG 6 cut(s) 323, 356, 357, 432, 537, 553
AgsI TTSAA 2 cut(s) 297, 331
AleI CACNNNNGTG 2 cut(s) 177, 458
AluBI AGCT 2 cut(s) 35, 536
AluI AGCT 2 cut(s) 35, 536
AlwI GGATC 4 cut(s) 109, 198, 225, 238
AoxI GGCC 6 cut(s) 24, 69, 183, 251, 314, 444
ApeKI GCWGC 5 cut(s) 35, 169, 267, 270, 346
ArsI GACNNNNNNTTYG 2 cut(s) 471, 503
AspLEI GCGC 2 cut(s) 106, 346
AspS9I GGNCC 5 cut(s) 109, 251, 314, 360, 445
AsuC2I CCSGG 4 cut(s) 304, 325, 351, 433
AsuHPI GGTGA 1 cut(s) 536
AvaII GGWCC 2 cut(s) 109, 360
BamHI GGATCC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 5 cut(s) 22, 181, 279, 282, 333
BccI CCATC 1 cut(s) 82
BceAI ACGGC 2 cut(s) 259, 459
BciVI GTATCC 1 cut(s) 254
BcnI CCSGG 4 cut(s) 304, 325, 351, 433
BfaI CTAG 1 cut(s) 548
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 9 cut(s) 21, 24, 36, 170, 204, 268, 271, 317, 347
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 9 cut(s) 22, 25, 37, 171, 205, 269, 272, 318, 348
Bme1390I CCNGG 4 cut(s) 304, 325, 351, 433
Bme18I GGWCC 2 cut(s) 109, 360
BmgT120I GGNCC 5 cut(s) 109, 251, 314, 360, 445
BmiI GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
BmrFI CCNGG 4 cut(s) 304, 325, 351, 433
BmrI ACTGGG 2 cut(s) 258, 321
BmuI ACTGGG 2 cut(s) 258, 321
BpiI GAAGAC 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 532
Bpu1102I GCTNAGC 1 cut(s) 165
BpuMI CCSGG 4 cut(s) 304, 325, 351, 433
BsaJI CCNNGG 6 cut(s) 21, 66, 159, 186, 303, 363
Bsc4I CCNNNNNNNGG 6 cut(s) 323, 356, 357, 432, 537, 553
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 2 cut(s) 253, 316
BseDI CCNNGG 6 cut(s) 21, 66, 159, 186, 303, 363
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 6 cut(s) 323, 356, 357, 432, 537, 553
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 253, 316
BseXI GCAGC 5 cut(s) 22, 181, 279, 282, 333
Bsh1236I CGCG 2 cut(s) 23, 344
Bsh1285I CGRYCG 2 cut(s) 414, 493
BshFI GGCC 6 cut(s) 26, 71, 185, 253, 316, 446
BshNI GGYRCC 1 cut(s) 428
BsiEI CGRYCG 2 cut(s) 414, 493
BsiSI CCGG 6 cut(s) 224, 303, 324, 351, 426, 432
BslFI GGGAC 2 cut(s) 373, 508
BslI CCNNNNNNNGG 6 cut(s) 323, 356, 357, 432, 537, 553
BsmFI GGGAC 2 cut(s) 373, 508
BsnI GGCC 6 cut(s) 26, 71, 185, 253, 316, 446
Bsp143I GATC 5 cut(s) 114, 190, 230, 411, 490
Bsp1720I GCTNAGC 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 186
BspANI GGCC 6 cut(s) 26, 71, 185, 253, 316, 446
BspCNI CTCAG 1 cut(s) 157
BspFNI CGCG 2 cut(s) 23, 344
BspLI GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
BspPI GGATC 4 cut(s) 109, 198, 225, 238
BspT107I GGYRCC 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 2 cut(s) 253, 316
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 6 cut(s) 21, 66, 159, 186, 303, 363
BssMI GATC 5 cut(s) 114, 190, 230, 411, 490
BssT1I CCWWGG 2 cut(s) 66, 186
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 2 cut(s) 165, 532
BstDSI CCRYGG 2 cut(s) 21, 186
BstENI CCTNNNNNAGG 1 cut(s) 551
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 2 cut(s) 23, 344
BstHHI GCGC 2 cut(s) 106, 346
BstKTI GATC 5 cut(s) 117, 193, 233, 414, 493
BstMBI GATC 5 cut(s) 114, 190, 230, 411, 490
BstMCI CGRYCG 2 cut(s) 414, 493
BstMWI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 284
BstSCI CCNGG 4 cut(s) 302, 323, 349, 431
BstUI CGCG 2 cut(s) 23, 344
BstV1I GCAGC 5 cut(s) 22, 181, 279, 282, 333
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 6 cut(s) 26, 71, 185, 253, 316, 446
BtgI CCRYGG 2 cut(s) 21, 186
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 376
CfoI GCGC 2 cut(s) 106, 346
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 5 cut(s) 109, 251, 314, 360, 445
Cfr42I CCGCGG 1 cut(s) 24
CviAII CATG 7 cut(s) 55, 76, 127, 187, 212, 356, 458
DdeI CTNAG 2 cut(s) 165, 532
DpnI GATC 5 cut(s) 116, 192, 232, 413, 492
DpnII GATC 5 cut(s) 114, 190, 230, 411, 490
EaeI YGGCCR 1 cut(s) 24
EciI GGCGGA 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 66, 186
Eco47I GGWCC 2 cut(s) 109, 360
EcoNI CCTNNNNNAGG 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 66, 186
ErhI CCWWGG 2 cut(s) 66, 186
FaeI CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
FaiI YATR 9 cut(s) 56, 77, 128, 188, 213, 292, 357, 450, 459
FaqI GGGAC 2 cut(s) 373, 508
FatI CATG 7 cut(s) 54, 75, 126, 186, 211, 355, 457
FauI CCCGC 3 cut(s) 266, 379, 481
Fnu4HI GCNGC 9 cut(s) 21, 24, 36, 170, 204, 268, 271, 317, 347
FokI GGATG 1 cut(s) 393
Fsp4HI GCNGC 9 cut(s) 21, 24, 36, 170, 204, 268, 271, 317, 347
FspBI CTAG 1 cut(s) 548
GlaI GCGC 2 cut(s) 105, 345
GluI GCNGC 9 cut(s) 21, 24, 36, 170, 204, 268, 271, 317, 347
HaeIII GGCC 6 cut(s) 26, 71, 185, 253, 316, 446
HapII CCGG 6 cut(s) 224, 303, 324, 351, 426, 432
HhaI GCGC 2 cut(s) 106, 346
Hin1II CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
Hin6I GCGC 2 cut(s) 104, 344
HinP1I GCGC 2 cut(s) 104, 344
HinfI GANTC 2 cut(s) 479, 561
HpaII CCGG 6 cut(s) 224, 303, 324, 351, 426, 432
HphI GGTGA 1 cut(s) 536
Hpy188I TCNGA 4 cut(s) 336, 411, 416, 470
Hpy99I CGWCG 2 cut(s) 259, 491
HpyAV CCTTC 2 cut(s) 94, 140
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 3 cut(s) 4, 79, 499
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 284
HpyF3I CTNAG 2 cut(s) 165, 532
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
HspAI GCGC 2 cut(s) 104, 344
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 5 cut(s) 114, 190, 230, 411, 490
Lsp1109I GCAGC 5 cut(s) 22, 181, 279, 282, 333
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 30
MalI GATC 5 cut(s) 116, 192, 232, 413, 492
MboI GATC 5 cut(s) 114, 190, 230, 411, 490
MboII GAAGA 3 cut(s) 73, 97, 106
MflI RGATCY 1 cut(s) 230
MlyI GAGTC 1 cut(s) 555
MmeI TCCRAC 1 cut(s) 493
MnlI CCTC 8 cut(s) 49, 154, 175, 358, 382, 429, 494, 514
MslI CAYNNNNRTG 3 cut(s) 177, 458, 462
MspA1I CMGCKG 3 cut(s) 23, 273, 374
MspI CCGG 6 cut(s) 224, 303, 324, 351, 426, 432
MspR9I CCNGG 4 cut(s) 304, 325, 351, 433
MvnI CGCG 2 cut(s) 23, 344
MwoI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 284
NciI CCSGG 4 cut(s) 304, 325, 351, 433
NcoI CCATGG 1 cut(s) 186
NdeII GATC 5 cut(s) 114, 190, 230, 411, 490
NlaIII CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
NlaIV GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
NmeAIII GCCGAG 2 cut(s) 184, 517
OliI CACNNNNGTG 2 cut(s) 177, 458
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 9 cut(s) 22, 25, 37, 171, 205, 269, 272, 318, 348
Ple19I CGATCG 2 cut(s) 414, 493
PleI GAGTC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 555
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
PspN4I GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
PspPI GGNCC 5 cut(s) 109, 251, 314, 360, 445
PspPPI RGGWCCY 1 cut(s) 109
PsuI RGATCY 1 cut(s) 230
PvuI CGATCG 2 cut(s) 414, 493
RseI CAYNNNNRTG 3 cut(s) 177, 458, 462
SacII CCGCGG 1 cut(s) 24
SatI GCNGC 9 cut(s) 21, 24, 36, 170, 204, 268, 271, 317, 347
Sau3AI GATC 5 cut(s) 114, 190, 230, 411, 490
Sau96I GGNCC 5 cut(s) 109, 251, 314, 360, 445
SchI GAGTC 1 cut(s) 555
ScrFI CCNGG 4 cut(s) 304, 325, 351, 433
SetI ASST 5 cut(s) 33, 37, 384, 538, 549
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 2 cut(s) 109, 360
SmiMI CAYNNNNRTG 3 cut(s) 177, 458, 462
SspMI CTAG 1 cut(s) 548
StyD4I CCNGG 4 cut(s) 302, 323, 349, 431
StyI CCWWGG 2 cut(s) 66, 186
TaiI ACGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 489
TaqII GACCGA 1 cut(s) 377
TauI GCSGC 4 cut(s) 23, 26, 206, 319
TfiI GAWTC 1 cut(s) 479
TscAI CASTG 1 cut(s) 253
TseI GCWGC 5 cut(s) 35, 169, 267, 270, 346
TspDTI ATGAA 2 cut(s) 113, 228
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 1 cut(s) 253
VpaK11BI GGWCC 2 cut(s) 109, 360
XagI CCTNNNNNAGG 1 cut(s) 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.