pycom12g05130

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013027
Physical Location & Seq
Forward (+)
5019815 .. 5020535
721 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05130.2

Sequence Viewer

Length: 510 bp
ATGCAGAGTGGGAAGAACACGATGGAGTCAATGAAAGAAGCAGCTGCTAACGTAGCAGCCTCTGCCACGTCTGGCATGGAAAAGACCAAGGCCACCGTGCAAGAAAAGGTGGAGAAGATGAAGTCCAGTGACCCAGTTGAAAAGGACATGGCGGCCCGAAAGAAAGAGGAGAGAATCGTCGAGGCCGAGGTCAACAAGCGTGAAGCGATGGAGCAAAACGCGGCGGCTAGGCGGGATGTGGCAGTGGCTACCGGCTATACTGCAACCGGTGCTGGCACTGGGACCCGCACGTATTCCACTACTGGGGCCGCAGGGCAGCCGACTGGAACTCACCAGATATCCGCAGTTCCCGGTCACGGCACCGGGCAGCCCGCGGGCCAAGTGACGGAGGGTGTGGTGGCGGAGGCGCACCCTATTGGGACCAACACGGGTACGGGTAGGACAACAGGCCATAATACTAACGTAGGTGGGAATGCAACCACTTATGGGACTGGTGGGACCTACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

170

Amino Acids

17.22

Weight (kDa)

7.97

Isoelectric Point (pI)

18.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 359
AccII CGCG 2 cut(s) 221, 374
AfaI GTAC 1 cut(s) 433
AfiI CCNNNNNNNGG 4 cut(s) 303, 356, 385, 486
AgeI ACCGGT 1 cut(s) 266
AgsI TTSAA 1 cut(s) 140
AjiI CACGTC 1 cut(s) 69
AluBI AGCT 1 cut(s) 44
AluI AGCT 1 cut(s) 44
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 6 cut(s) 90, 153, 183, 306, 376, 448
ApeKI GCWGC 5 cut(s) 41, 44, 56, 316, 367
AsiGI ACCGGT 1 cut(s) 266
AspLEI GCGC 1 cut(s) 409
AspS9I GGNCC 6 cut(s) 154, 282, 306, 376, 420, 498
AsuC2I CCSGG 2 cut(s) 351, 364
AsuHPI GGTGA 1 cut(s) 323
AvaII GGWCC 3 cut(s) 282, 420, 498
BanI GGYRCC 1 cut(s) 359
BbvI GCAGC 5 cut(s) 31, 53, 68, 328, 379
BccI CCATC 2 cut(s) 16, 202
BceAI ACGGC 1 cut(s) 373
BcnI CCSGG 2 cut(s) 351, 364
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 502
BisI GCNGC 9 cut(s) 42, 45, 57, 153, 222, 225, 309, 317, 368
BlsI GCNGC 9 cut(s) 43, 46, 58, 154, 223, 226, 310, 318, 369
Bme1390I CCNGG 2 cut(s) 351, 364
Bme18I GGWCC 3 cut(s) 282, 420, 498
BmgBI CACGTC 1 cut(s) 69
BmgT120I GGNCC 6 cut(s) 154, 282, 306, 376, 420, 498
BmiI GGNNCC 6 cut(s) 283, 284, 307, 361, 421, 499
BmrFI CCNGG 2 cut(s) 351, 364
BmrI ACTGGG 3 cut(s) 128, 288, 312
BmuI ACTGGG 3 cut(s) 128, 288, 312
BpuMI CCSGG 2 cut(s) 351, 364
BsaAI YACGTR 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 87, 186, 372
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 4 cut(s) 303, 356, 385, 486
Bse118I RCCGGY 2 cut(s) 251, 266
Bse1I ACTGG 6 cut(s) 126, 134, 283, 307, 328, 496
BseDI CCNNGG 3 cut(s) 87, 186, 372
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 4 cut(s) 303, 356, 385, 486
BseNI ACTGG 6 cut(s) 126, 134, 283, 307, 328, 496
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 5 cut(s) 31, 53, 68, 328, 379
Bsh1236I CGCG 2 cut(s) 221, 374
BshFI GGCC 6 cut(s) 92, 155, 185, 308, 378, 450
BshNI GGYRCC 1 cut(s) 359
BshTI ACCGGT 1 cut(s) 266
BsiSI CCGG 4 cut(s) 252, 267, 351, 363
BslFI GGGAC 3 cut(s) 295, 433, 502
BslI CCNNNNNNNGG 4 cut(s) 303, 356, 385, 486
BsmFI GGGAC 3 cut(s) 295, 433, 502
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 6 cut(s) 92, 155, 185, 308, 378, 450
BspANI GGCC 6 cut(s) 92, 155, 185, 308, 378, 450
BspFNI CGCG 2 cut(s) 221, 374
BspLI GGNNCC 6 cut(s) 283, 284, 307, 361, 421, 499
BspT107I GGYRCC 1 cut(s) 359
BsrFI RCCGGY 2 cut(s) 251, 266
BsrI ACTGG 6 cut(s) 126, 134, 283, 307, 328, 496
BssAI RCCGGY 2 cut(s) 251, 266
BssECI CCNNGG 3 cut(s) 87, 186, 372
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 97, 506
BstAPI GCANNNNNTGC 2 cut(s) 62, 269
BstBAI YACGTR 1 cut(s) 291
BstC8I GCNNGC 3 cut(s) 274, 372, 376
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 1 cut(s) 241
BstFNI CGCG 2 cut(s) 221, 374
BstHHI GCGC 1 cut(s) 409
BstMWI GCNNNNNNNGC 3 cut(s) 53, 62, 269
BstSCI CCNGG 2 cut(s) 349, 362
BstSFI CTRYAG 1 cut(s) 502
BstUI CGCG 2 cut(s) 221, 374
BstV1I GCAGC 5 cut(s) 31, 53, 68, 328, 379
BsuRI GGCC 6 cut(s) 92, 155, 185, 308, 378, 450
BtgI CCRYGG 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 221
BtrI CACGTC 1 cut(s) 69
BtsCI GGATG 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 3 cut(s) 133, 249, 276
Cac8I GCNNGC 3 cut(s) 274, 372, 376
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 1 cut(s) 409
Cfr10I RCCGGY 2 cut(s) 251, 266
Cfr13I GGNCC 6 cut(s) 154, 282, 306, 376, 420, 498
Cfr42I CCGCGG 1 cut(s) 375
Csp6I GTAC 1 cut(s) 432
CspAI ACCGGT 1 cut(s) 266
CviAII CATG 2 cut(s) 76, 148
CviQI GTAC 1 cut(s) 432
EciI GGCGGA 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 87
Eco32I GATATC 1 cut(s) 339
Eco47I GGWCC 3 cut(s) 282, 420, 498
EcoO109I RGGNCCY 2 cut(s) 282, 498
EcoRV GATATC 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 79, 151
FaiI YATR 5 cut(s) 77, 149, 258, 453, 486
FaqI GGGAC 3 cut(s) 295, 433, 502
FatI CATG 2 cut(s) 75, 147
FauI CCCGC 4 cut(s) 225, 293, 367, 379
Fnu4HI GCNGC 9 cut(s) 42, 45, 57, 153, 222, 225, 309, 317, 368
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 9 cut(s) 42, 45, 57, 153, 222, 225, 309, 317, 368
FspBI CTAG 1 cut(s) 228
GlaI GCGC 1 cut(s) 408
GluI GCNGC 9 cut(s) 42, 45, 57, 153, 222, 225, 309, 317, 368
HaeIII GGCC 6 cut(s) 92, 155, 185, 308, 378, 450
HapII CCGG 4 cut(s) 252, 267, 351, 363
HhaI GCGC 1 cut(s) 409
Hin1II CATG 2 cut(s) 79, 151
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 2 cut(s) 26, 174
HpaII CCGG 4 cut(s) 252, 267, 351, 363
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 193
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 1 cut(s) 182
HpyCH4III ACNGT 2 cut(s) 97, 506
HpyCH4IV ACGT 4 cut(s) 51, 68, 290, 462
HpyCH4V TGCA 4 cut(s) 4, 100, 263, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 62, 269
HpySE526I ACGT 4 cut(s) 51, 68, 290, 462
Hsp92II CATG 2 cut(s) 79, 151
HspAI GCGC 1 cut(s) 407
KflI GGGWCCC 1 cut(s) 282
KspI CCGCGG 1 cut(s) 375
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 5 cut(s) 31, 53, 68, 328, 379
MaeI CTAG 1 cut(s) 228
MaeII ACGT 4 cut(s) 51, 68, 290, 462
MaeIII GTNAC 3 cut(s) 128, 353, 382
MboII GAAGA 2 cut(s) 25, 127
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 6 cut(s) 70, 160, 175, 181, 382, 397
MspA1I CMGCKG 2 cut(s) 44, 374
MspI CCGG 4 cut(s) 252, 267, 351, 363
MspR9I CCNGG 2 cut(s) 351, 364
Mva1269I GAATGC 1 cut(s) 478
MvnI CGCG 2 cut(s) 221, 374
MwoI GCNNNNNNNGC 3 cut(s) 53, 62, 269
NciI CCSGG 2 cut(s) 351, 364
NlaIII CATG 2 cut(s) 79, 151
NlaIV GGNNCC 6 cut(s) 283, 284, 307, 361, 421, 499
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 3 cut(s) 128, 353, 382
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 478
PfeI GAWTC 1 cut(s) 174
PinAI ACCGGT 1 cut(s) 266
PkrI GCNGC 9 cut(s) 43, 46, 58, 154, 223, 226, 310, 318, 369
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Ppu21I YACGTR 1 cut(s) 291
PpuMI RGGWCCY 2 cut(s) 282, 498
Psp5II RGGWCCY 2 cut(s) 282, 498
PspN4I GGNNCC 6 cut(s) 283, 284, 307, 361, 421, 499
PspPI GGNCC 6 cut(s) 154, 282, 306, 376, 420, 498
PspPPI RGGWCCY 2 cut(s) 282, 498
PstNI CAGNNNCTG 1 cut(s) 62
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
SacII CCGCGG 1 cut(s) 375
SatI GCNGC 9 cut(s) 42, 45, 57, 153, 222, 225, 309, 317, 368
Sau96I GGNCC 6 cut(s) 154, 282, 306, 376, 420, 498
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 2 cut(s) 351, 364
SetI ASST 9 cut(s) 46, 54, 71, 111, 192, 293, 465, 469, 503
SfcI CTRYAG 1 cut(s) 502
Sfr303I CCGCGG 1 cut(s) 375
SgrBI CCGCGG 1 cut(s) 375
SinI GGWCC 3 cut(s) 282, 420, 498
SspMI CTAG 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 349, 362
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 97, 506
TaiI ACGT 4 cut(s) 54, 71, 293, 465
TaqI TCGA 1 cut(s) 180
TauI GCSGC 4 cut(s) 155, 224, 227, 311
TfiI GAWTC 1 cut(s) 174
TscAI CASTG 3 cut(s) 133, 249, 283
TseFI GTSAC 3 cut(s) 128, 353, 382
TseI GCWGC 5 cut(s) 41, 44, 56, 316, 367
Tsp45I GTSAC 3 cut(s) 128, 353, 382
TspDTI ATGAA 2 cut(s) 47, 134
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 3 cut(s) 133, 249, 283
VpaK11BI GGWCC 3 cut(s) 282, 420, 498
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.