RchiOBHm_Chr3g0479771

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0013027
Physical Location & Seq
Forward (+)
25548278 .. 25549152
875 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44483

Sequence Viewer

Length: 507 bp
ATGCAGTCAATCAAGGAAGCCGCGGCCAACGTAGCTGCCTCTGCCAAGTCTGGCATGGAGAAGACCAAGGCCACCATGCAGGAGAAGATGGAGAAGATGAAGGCACAGGACCCGATCCAAAAGGACATGGCAACTCAAAAGAAAGAAGGGCGGATTGCCGAGGCTGAGCAGCACAAGCGTGAGGCCATGGATCACAACACAGCCTCTAAACATGGAACAGCTCCGGCTGGATCCACAGGCTACGGCACTGGGCCGACGGGATACAGCACCAGCGGGGTTGGAACAGCCACATATCTAACCACCGGGGCTACTGGGCAGCAAACCGGGTTCAATCAGATGTCCGCACTGCCCGGACATGGGACCGGGGAACCCGCTGGCCAGGTGGTTGAGGGTGTTGTCACCGCACATCCGACTGGAACCAACACCGGCACCGGGAGGACTACGGCCCATAACACCCATGTGGGTGGTAATGCCCCCGGCTATGGGACTGGCGGGACTTATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

168

Amino Acids

17.06

Weight (kDa)

8.81

Isoelectric Point (pI)

16.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 5.2e-20 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 428
AccII CGCG 1 cut(s) 23
AciI CCGC 8 cut(s) 21, 23, 151, 273, 342, 372, 402, 492
AclWI GGATC 4 cut(s) 109, 198, 225, 238
AcoI YGGCCR 2 cut(s) 24, 376
AfiI CCNNNNNNNGG 5 cut(s) 356, 357, 432, 482, 483
AgsI TTSAA 1 cut(s) 331
AjnI CCWGG 1 cut(s) 378
AleI CACNNNNGTG 2 cut(s) 177, 458
AluBI AGCT 2 cut(s) 35, 221
AluI AGCT 2 cut(s) 35, 221
AlwI GGATC 4 cut(s) 109, 198, 225, 238
AoxI GGCC 6 cut(s) 24, 69, 183, 251, 376, 444
ApeKI GCWGC 3 cut(s) 35, 169, 316
AspS9I GGNCC 4 cut(s) 109, 251, 360, 445
AsuC2I CCSGG 6 cut(s) 304, 325, 351, 364, 433, 477
AsuHPI GGTGA 1 cut(s) 391
AvaII GGWCC 2 cut(s) 109, 360
BalI TGGCCA 1 cut(s) 378
BamHI GGATCC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 22, 181, 328
BccI CCATC 1 cut(s) 82
BceAI ACGGC 2 cut(s) 259, 459
BciT130I CCWGG 1 cut(s) 380
BciVI GTATCC 1 cut(s) 254
BcnI CCSGG 6 cut(s) 304, 325, 351, 364, 433, 477
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 5 cut(s) 21, 24, 36, 170, 317
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 5 cut(s) 22, 25, 37, 171, 318
Bme1390I CCNGG 7 cut(s) 304, 325, 351, 364, 380, 433, 477
Bme18I GGWCC 2 cut(s) 109, 360
BmgT120I GGNCC 4 cut(s) 109, 251, 360, 445
BmiI GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
BmrFI CCNGG 7 cut(s) 304, 325, 351, 364, 380, 433, 477
BmrI ACTGGG 2 cut(s) 258, 321
BmuI ACTGGG 2 cut(s) 258, 321
BpiI GAAGAC 1 cut(s) 68
Bpu1102I GCTNAGC 1 cut(s) 165
BpuMI CCSGG 6 cut(s) 304, 325, 351, 364, 433, 477
BsaJI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
Bsc4I CCNNNNNNNGG 5 cut(s) 356, 357, 432, 482, 483
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 4 cut(s) 253, 316, 418, 493
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 5 cut(s) 356, 357, 432, 482, 483
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 4 cut(s) 253, 316, 418, 493
BseXI GCAGC 3 cut(s) 22, 181, 328
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 6 cut(s) 26, 71, 185, 253, 378, 446
BshNI GGYRCC 1 cut(s) 428
BsiSI CCGG 8 cut(s) 224, 303, 324, 351, 363, 426, 432, 477
BslFI GGGAC 2 cut(s) 373, 499
BslI CCNNNNNNNGG 5 cut(s) 356, 357, 432, 482, 483
BsmFI GGGAC 2 cut(s) 373, 499
BsnI GGCC 6 cut(s) 26, 71, 185, 253, 378, 446
Bsp143I GATC 3 cut(s) 114, 190, 230
Bsp1720I GCTNAGC 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 8 cut(s) 21, 23, 151, 273, 342, 372, 402, 492
BspANI GGCC 6 cut(s) 26, 71, 185, 253, 378, 446
BspCNI CTCAG 1 cut(s) 157
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
BspPI GGATC 4 cut(s) 109, 198, 225, 238
BspT107I GGYRCC 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 4 cut(s) 253, 316, 418, 493
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
BssMI GATC 3 cut(s) 114, 190, 230
BssT1I CCWWGG 2 cut(s) 66, 186
Bst2UI CCWGG 1 cut(s) 380
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 1 cut(s) 165
BstDSI CCRYGG 2 cut(s) 21, 186
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 3 cut(s) 117, 193, 233
BstMBI GATC 3 cut(s) 114, 190, 230
BstMWI GCNNNNNNNGC 3 cut(s) 32, 41, 175
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 7 cut(s) 302, 323, 349, 362, 378, 431, 475
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 22, 181, 328
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 1 cut(s) 230
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 6 cut(s) 26, 71, 185, 253, 378, 446
BtgI CCRYGG 2 cut(s) 21, 186
BtsCI GGATG 1 cut(s) 406
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 2 cut(s) 246, 344
Cac8I GCNNGC 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 4 cut(s) 109, 251, 360, 445
Cfr42I CCGCGG 1 cut(s) 24
CviAII CATG 7 cut(s) 55, 76, 127, 187, 212, 356, 458
DdeI CTNAG 1 cut(s) 165
DpnI GATC 3 cut(s) 116, 192, 232
DpnII GATC 3 cut(s) 114, 190, 230
EaeI YGGCCR 2 cut(s) 24, 376
EciI GGCGGA 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 66, 186
Eco47I GGWCC 2 cut(s) 109, 360
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 2 cut(s) 66, 186
ErhI CCWWGG 2 cut(s) 66, 186
FaeI CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
FaqI GGGAC 2 cut(s) 373, 499
FatI CATG 7 cut(s) 54, 75, 126, 186, 211, 355, 457
FauI CCCGC 3 cut(s) 266, 379, 485
Fnu4HI GCNGC 5 cut(s) 21, 24, 36, 170, 317
FokI GGATG 1 cut(s) 393
Fsp4HI GCNGC 5 cut(s) 21, 24, 36, 170, 317
GluI GCNGC 5 cut(s) 21, 24, 36, 170, 317
HaeIII GGCC 6 cut(s) 26, 71, 185, 253, 378, 446
HapII CCGG 8 cut(s) 224, 303, 324, 351, 363, 426, 432, 477
Hin1II CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
HpaII CCGG 8 cut(s) 224, 303, 324, 351, 363, 426, 432, 477
HphI GGTGA 1 cut(s) 391
Hpy188I TCNGA 2 cut(s) 336, 411
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 94, 140
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 2 cut(s) 4, 79
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 41, 175
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 3 cut(s) 114, 190, 230
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 3 cut(s) 22, 181, 328
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 397
MalI GATC 3 cut(s) 116, 192, 232
MboI GATC 3 cut(s) 114, 190, 230
MboII GAAGA 3 cut(s) 73, 97, 106
MflI RGATCY 1 cut(s) 230
MlsI TGGCCA 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 378
MmeI TCCRAC 2 cut(s) 259, 434
MnlI CCTC 6 cut(s) 49, 154, 175, 214, 382, 429
Mox20I TGGCCA 1 cut(s) 378
MscI TGGCCA 1 cut(s) 378
MslI CAYNNNNRTG 3 cut(s) 177, 458, 462
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 3 cut(s) 23, 273, 374
MspI CCGG 8 cut(s) 224, 303, 324, 351, 363, 426, 432, 477
MspR9I CCNGG 7 cut(s) 304, 325, 351, 364, 380, 433, 477
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 32, 41, 175
NciI CCSGG 6 cut(s) 304, 325, 351, 364, 433, 477
NcoI CCATGG 1 cut(s) 186
NdeII GATC 3 cut(s) 114, 190, 230
NlaIII CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
NlaIV GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 397
OliI CACNNNNGTG 2 cut(s) 177, 458
PkrI GCNGC 5 cut(s) 22, 25, 37, 171, 318
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
PspPI GGNCC 4 cut(s) 109, 251, 360, 445
PspPPI RGGWCCY 1 cut(s) 109
PsuI RGATCY 1 cut(s) 230
RseI CAYNNNNRTG 3 cut(s) 177, 458, 462
SacII CCGCGG 1 cut(s) 24
SatI GCNGC 5 cut(s) 21, 24, 36, 170, 317
Sau3AI GATC 3 cut(s) 114, 190, 230
Sau96I GGNCC 4 cut(s) 109, 251, 360, 445
ScrFI CCNGG 7 cut(s) 304, 325, 351, 364, 380, 433, 477
SetI ASST 4 cut(s) 33, 37, 223, 384
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 2 cut(s) 109, 360
SmiMI CAYNNNNRTG 3 cut(s) 177, 458, 462
SsiI CCGC 8 cut(s) 21, 23, 151, 273, 342, 372, 402, 492
StyD4I CCNGG 7 cut(s) 302, 323, 349, 362, 378, 431, 475
StyI CCWWGG 2 cut(s) 66, 186
TaiI ACGT 1 cut(s) 33
TauI GCSGC 2 cut(s) 23, 26
TscAI CASTG 2 cut(s) 253, 351
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 3 cut(s) 35, 169, 316
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 113
TspRI CASTG 2 cut(s) 253, 351
VpaK11BI GGWCC 2 cut(s) 109, 360
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.