MD12G1057800.v1.1

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013027
Physical Location & Seq
Forward (+)
6598564 .. 6599696
1133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1057800.v1.1.491

Sequence Viewer

Length: 498 bp
ATGCAGAGTGGGAAGAACGCAATGAAAGAAGCAGCTGCTAACGTAGCAGCCTCTGCCATGTCTGGCATGGAAAAGACCAAAGCCACCGTGCAAGAAAAGGTGGAGAAGATGAAGTCCAGTGACCCAGTTGAAAAGGACATGGCGGCCCGAAAGAAAGAGGAGAGAATAGTCGAGGCCGAGGTCAACAAGCATGAAGCGATGGAGCAAAACGCGGCGGCTAGGCGGGATGTGGCAGTGGCTACCGGCTATACTGCGACCGGTGCTGGCACTGGGACCCACACGTATTCCACGACTGGGGCCGCAGGGCAGCCGACTGGAACTCACCAGATGTCCGCACTTCCCGGTCACGGCACCGGGCAGCCCGCTGGCCAAGTGACGGAGGGTGTGGCGGAGGCGCACCCTATTGGGACCAACACGGGTACGGGTCGGACAACAGCCCATAATACTAATGTAGGTGGGAATGCGACCAAGTACGGGACCGGTGGGACCTACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

166

Amino Acids

16.81

Weight (kDa)

7.99

Isoelectric Point (pI)

20.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 7 - 74 7.7e-18 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 350
AccII CGCG 1 cut(s) 212
AciI CCGC 8 cut(s) 143, 212, 215, 223, 300, 333, 363, 389
AcoI YGGCCR 1 cut(s) 367
AfaI GTAC 2 cut(s) 421, 473
AfiI CCNNNNNNNGG 4 cut(s) 294, 347, 376, 474
AflIII ACRYGT 1 cut(s) 279
AgeI ACCGGT 2 cut(s) 257, 479
AgsI TTSAA 1 cut(s) 131
AluBI AGCT 1 cut(s) 35
AluI AGCT 1 cut(s) 35
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 4 cut(s) 144, 174, 297, 367
ApeKI GCWGC 5 cut(s) 32, 35, 47, 307, 358
AsiGI ACCGGT 2 cut(s) 257, 479
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 6 cut(s) 145, 273, 297, 408, 477, 486
AsuC2I CCSGG 2 cut(s) 342, 355
AsuHPI GGTGA 1 cut(s) 314
AvaII GGWCC 4 cut(s) 273, 408, 477, 486
BalI TGGCCA 1 cut(s) 369
BanI GGYRCC 1 cut(s) 350
BbvI GCAGC 5 cut(s) 22, 44, 59, 319, 370
BccI CCATC 1 cut(s) 193
BceAI ACGGC 1 cut(s) 364
BcnI CCSGG 2 cut(s) 342, 355
BfaI CTAG 1 cut(s) 219
BfmI CTRYAG 1 cut(s) 490
BisI GCNGC 9 cut(s) 33, 36, 48, 144, 213, 216, 300, 308, 359
BlsI GCNGC 9 cut(s) 34, 37, 49, 145, 214, 217, 301, 309, 360
Bme1390I CCNGG 2 cut(s) 342, 355
Bme18I GGWCC 4 cut(s) 273, 408, 477, 486
BmgT120I GGNCC 6 cut(s) 145, 273, 297, 408, 477, 486
BmiI GGNNCC 7 cut(s) 274, 275, 298, 352, 409, 478, 487
BmrFI CCNGG 2 cut(s) 342, 355
BmrI ACTGGG 3 cut(s) 119, 279, 303
BmuI ACTGGG 3 cut(s) 119, 279, 303
BpuMI CCSGG 2 cut(s) 342, 355
BsaAI YACGTR 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 2 cut(s) 257, 479
Bsc4I CCNNNNNNNGG 4 cut(s) 294, 347, 376, 474
Bse118I RCCGGY 3 cut(s) 242, 257, 479
Bse1I ACTGG 5 cut(s) 117, 125, 274, 298, 319
Bse3DI GCAATG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 4 cut(s) 294, 347, 376, 474
BseMI GCAATG 1 cut(s) 27
BseNI ACTGG 5 cut(s) 117, 125, 274, 298, 319
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 5 cut(s) 22, 44, 59, 319, 370
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 4 cut(s) 146, 176, 299, 369
BshNI GGYRCC 1 cut(s) 350
BshTI ACCGGT 2 cut(s) 257, 479
BsiEI CGRYCG 1 cut(s) 258
BsiSI CCGG 5 cut(s) 243, 258, 342, 354, 480
BslFI GGGAC 3 cut(s) 286, 421, 490
BslI CCNNNNNNNGG 4 cut(s) 294, 347, 376, 474
BsmFI GGGAC 3 cut(s) 286, 421, 490
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 4 cut(s) 146, 176, 299, 369
BspACI CCGC 8 cut(s) 143, 212, 215, 223, 300, 333, 363, 389
BspANI GGCC 4 cut(s) 146, 176, 299, 369
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 7 cut(s) 274, 275, 298, 352, 409, 478, 487
BspT107I GGYRCC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 3 cut(s) 242, 257, 479
BsrI ACTGG 5 cut(s) 117, 125, 274, 298, 319
BssAI RCCGGY 3 cut(s) 242, 257, 479
BssECI CCNNGG 1 cut(s) 177
Bst4CI ACNGT 2 cut(s) 88, 494
BstAPI GCANNNNNTGC 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 265, 363, 367
BstF5I GGATG 1 cut(s) 232
BstFNI CGCG 1 cut(s) 212
BstHHI GCGC 1 cut(s) 397
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 3 cut(s) 44, 53, 260
BstSCI CCNGG 2 cut(s) 340, 353
BstSFI CTRYAG 1 cut(s) 490
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 5 cut(s) 22, 44, 59, 319, 370
BsuRI GGCC 4 cut(s) 146, 176, 299, 369
BtgZI GCGATG 1 cut(s) 212
BtsCI GGATG 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 3 cut(s) 124, 240, 267
Cac8I GCNNGC 3 cut(s) 265, 363, 367
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 1 cut(s) 397
Cfr10I RCCGGY 3 cut(s) 242, 257, 479
Cfr13I GGNCC 6 cut(s) 145, 273, 297, 408, 477, 486
Csp6I GTAC 2 cut(s) 420, 472
CspAI ACCGGT 2 cut(s) 257, 479
CviAII CATG 4 cut(s) 58, 67, 139, 191
CviQI GTAC 2 cut(s) 420, 472
EaeI YGGCCR 1 cut(s) 367
EciI GGCGGA 1 cut(s) 404
Eco47I GGWCC 4 cut(s) 273, 408, 477, 486
EcoO109I RGGNCCY 2 cut(s) 273, 486
FaeI CATG 4 cut(s) 61, 70, 142, 194
FaiI YATR 6 cut(s) 59, 68, 140, 192, 249, 441
FaqI GGGAC 3 cut(s) 286, 421, 490
FatI CATG 4 cut(s) 57, 66, 138, 190
FauI CCCGC 2 cut(s) 216, 370
Fnu4HI GCNGC 9 cut(s) 33, 36, 48, 144, 213, 216, 300, 308, 359
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 9 cut(s) 33, 36, 48, 144, 213, 216, 300, 308, 359
FspBI CTAG 1 cut(s) 219
GlaI GCGC 1 cut(s) 396
GluI GCNGC 9 cut(s) 33, 36, 48, 144, 213, 216, 300, 308, 359
HaeIII GGCC 4 cut(s) 146, 176, 299, 369
HapII CCGG 5 cut(s) 243, 258, 342, 354, 480
HhaI GCGC 1 cut(s) 397
Hin1II CATG 4 cut(s) 61, 70, 142, 194
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HpaII CCGG 5 cut(s) 243, 258, 342, 354, 480
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 1 cut(s) 429
Hpy8I GTNNAC 1 cut(s) 184
HpyCH4III ACNGT 2 cut(s) 88, 494
HpyCH4IV ACGT 2 cut(s) 42, 281
HpyCH4V TGCA 2 cut(s) 4, 91
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 53, 260
HpySE526I ACGT 2 cut(s) 42, 281
Hsp92II CATG 4 cut(s) 61, 70, 142, 194
HspAI GCGC 1 cut(s) 395
KflI GGGWCCC 1 cut(s) 273
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 5 cut(s) 22, 44, 59, 319, 370
MaeI CTAG 1 cut(s) 219
MaeII ACGT 2 cut(s) 42, 281
MaeIII GTNAC 3 cut(s) 119, 344, 373
MboII GAAGA 2 cut(s) 25, 118
MlsI TGGCCA 1 cut(s) 369
MluNI TGGCCA 1 cut(s) 369
MmeI TCCRAC 1 cut(s) 407
MnlI CCTC 6 cut(s) 61, 151, 166, 172, 373, 385
Mox20I TGGCCA 1 cut(s) 369
MscI TGGCCA 1 cut(s) 369
Msp20I TGGCCA 1 cut(s) 369
MspA1I CMGCKG 2 cut(s) 35, 365
MspI CCGG 5 cut(s) 243, 258, 342, 354, 480
MspR9I CCNGG 2 cut(s) 342, 355
Mva1269I GAATGC 1 cut(s) 466
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 3 cut(s) 44, 53, 260
NciI CCSGG 2 cut(s) 342, 355
NlaIII CATG 4 cut(s) 61, 70, 142, 194
NlaIV GGNNCC 7 cut(s) 274, 275, 298, 352, 409, 478, 487
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 3 cut(s) 119, 344, 373
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PctI GAATGC 1 cut(s) 466
PinAI ACCGGT 2 cut(s) 257, 479
PkrI GCNGC 9 cut(s) 34, 37, 49, 145, 214, 217, 301, 309, 360
Ppu21I YACGTR 1 cut(s) 282
PpuMI RGGWCCY 2 cut(s) 273, 486
Psp5II RGGWCCY 2 cut(s) 273, 486
PspN4I GGNNCC 7 cut(s) 274, 275, 298, 352, 409, 478, 487
PspPI GGNCC 6 cut(s) 145, 273, 297, 408, 477, 486
PspPPI RGGWCCY 2 cut(s) 273, 486
PstNI CAGNNNCTG 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 2 cut(s) 421, 473
RsaNI GTAC 2 cut(s) 420, 472
SatI GCNGC 9 cut(s) 33, 36, 48, 144, 213, 216, 300, 308, 359
Sau96I GGNCC 6 cut(s) 145, 273, 297, 408, 477, 486
ScrFI CCNGG 2 cut(s) 342, 355
SetI ASST 7 cut(s) 37, 45, 102, 183, 284, 457, 491
SfcI CTRYAG 1 cut(s) 490
SinI GGWCC 4 cut(s) 273, 408, 477, 486
SsiI CCGC 8 cut(s) 143, 212, 215, 223, 300, 333, 363, 389
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 340, 353
TaaI ACNGT 2 cut(s) 88, 494
TaiI ACGT 2 cut(s) 45, 284
TaqI TCGA 1 cut(s) 171
TauI GCSGC 4 cut(s) 146, 215, 218, 302
TscAI CASTG 3 cut(s) 124, 240, 274
TseFI GTSAC 3 cut(s) 119, 344, 373
TseI GCWGC 5 cut(s) 32, 35, 47, 307, 358
Tsp45I GTSAC 3 cut(s) 119, 344, 373
TspDTI ATGAA 3 cut(s) 38, 125, 207
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 3 cut(s) 124, 240, 274
VpaK11BI GGWCC 4 cut(s) 273, 408, 477, 486
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.