pycom14g04720

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013027
Physical Location & Seq
Forward (+)
4454241 .. 4455118
878 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04720.1

Sequence Viewer

Length: 507 bp
ATGCAAAGCGGGAAGAACGCGATGGAGTCGATAAAAGAAGCAGCTGCTAATATAGCAGCCTCTGCTATCTCTGGCATGGAAAAGACCAAACCCACCGTGCAAGAAAAGGTGGAGAAGATGAAATCCAGTGACCCAATTGAAAAGGACATGGCGGCCGGAAAGAAAGAGGAGAGAATAGTCGATGCCGAGATCAACAAACGTAATGCCATGGAGCAAAACGCCGCGGCTCGGCGGGATGTGGCAGGGGCTACCGGCTATACTGCGACCGGTGCTGGCACTGGAACCCACACGTATTCTACGACTGGGGCCACAGGGCAGCAGACTGGAACTCACCAGATATCCGCACTACCTGGCCACGGCACCGGTCAGCCTGCGGGCCAAGTGACGGAGGGTGCGGTGGGCGCGCACCCTGTTGGGATCAACACGGGTACGGGTCGGACCACAGCCCACAACACTCATGTTGGCGGGAATGTACCTAACTATGGGACTGGTGGGACCTATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

169

Amino Acids

17.06

Weight (kDa)

8.0

Isoelectric Point (pI)

22.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 8 - 77 1.4e-17 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 359
AccII CGCG 3 cut(s) 20, 224, 404
AciI CCGC 9 cut(s) 9, 152, 222, 224, 232, 342, 374, 395, 465
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 2 cut(s) 153, 352
AfaI GTAC 2 cut(s) 430, 474
AfiI CCNNNNNNNGG 4 cut(s) 228, 356, 385, 482
AflIII ACRYGT 1 cut(s) 288
AgeI ACCGGT 2 cut(s) 266, 362
AgsI TTSAA 1 cut(s) 140
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 1 cut(s) 44
AluI AGCT 1 cut(s) 44
AlwI GGATC 1 cut(s) 425
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 4 cut(s) 153, 306, 352, 376
ApeKI GCWGC 4 cut(s) 41, 44, 56, 316
AsiGI ACCGGT 2 cut(s) 266, 362
AspLEI GCGC 2 cut(s) 404, 406
AspS9I GGNCC 4 cut(s) 306, 376, 438, 495
AsuHPI GGTGA 1 cut(s) 323
AvaII GGWCC 2 cut(s) 438, 495
BalI TGGCCA 1 cut(s) 354
BanI GGYRCC 1 cut(s) 359
BbvI GCAGC 4 cut(s) 31, 53, 68, 328
BccI CCATC 1 cut(s) 16
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 351
BisI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
BlsI GCNGC 7 cut(s) 43, 46, 58, 154, 223, 226, 318
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 2 cut(s) 438, 495
BmgT120I GGNCC 4 cut(s) 306, 376, 438, 495
BmiI GGNNCC 4 cut(s) 283, 307, 361, 496
BmrFI CCNGG 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 312
BmsI GCATC 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 207, 222, 355
BsaWI WCCGGW 2 cut(s) 266, 362
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 356, 385, 482
Bse118I RCCGGY 3 cut(s) 251, 266, 362
Bse1I ACTGG 5 cut(s) 126, 283, 307, 328, 493
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 3 cut(s) 207, 222, 355
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 4 cut(s) 228, 356, 385, 482
BseNI ACTGG 5 cut(s) 126, 283, 307, 328, 493
BsePI GCGCGC 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 182
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 4 cut(s) 31, 53, 68, 328
Bsh1236I CGCG 3 cut(s) 20, 224, 404
Bsh1285I CGRYCG 2 cut(s) 156, 267
BshFI GGCC 4 cut(s) 155, 308, 354, 378
BshNI GGYRCC 1 cut(s) 359
BshTI ACCGGT 2 cut(s) 266, 362
BsiEI CGRYCG 2 cut(s) 156, 267
BsiSI CCGG 4 cut(s) 156, 252, 267, 363
BslFI GGGAC 1 cut(s) 499
BslI CCNNNNNNNGG 4 cut(s) 228, 356, 385, 482
BsmFI GGGAC 1 cut(s) 499
BsnI GGCC 4 cut(s) 155, 308, 354, 378
Bsp143I GATC 2 cut(s) 189, 417
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 9 cut(s) 9, 152, 222, 224, 232, 342, 374, 395, 465
BspANI GGCC 4 cut(s) 155, 308, 354, 378
BspFNI CGCG 3 cut(s) 20, 224, 404
BspLI GGNNCC 4 cut(s) 283, 307, 361, 496
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 359
BsrFI RCCGGY 3 cut(s) 251, 266, 362
BsrI ACTGG 5 cut(s) 126, 283, 307, 328, 493
BssAI RCCGGY 3 cut(s) 251, 266, 362
BssECI CCNNGG 3 cut(s) 207, 222, 355
BssHII GCGCGC 1 cut(s) 402
BssMI GATC 2 cut(s) 189, 417
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 291
BstC8I GCNNGC 4 cut(s) 274, 372, 376, 404
BstDSI CCRYGG 3 cut(s) 207, 222, 355
BstF5I GGATG 1 cut(s) 241
BstFNI CGCG 3 cut(s) 20, 224, 404
BstHHI GCGC 2 cut(s) 404, 406
BstKTI GATC 2 cut(s) 192, 420
BstMBI GATC 2 cut(s) 189, 417
BstMCI CGRYCG 2 cut(s) 156, 267
BstMWI GCNNNNNNNGC 4 cut(s) 53, 62, 269, 401
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstUI CGCG 3 cut(s) 20, 224, 404
BstV1I GCAGC 4 cut(s) 31, 53, 68, 328
BstZI CGGCCG 1 cut(s) 153
BsuRI GGCC 4 cut(s) 155, 308, 354, 378
BtgI CCRYGG 3 cut(s) 207, 222, 355
BtgZI GCGATG 1 cut(s) 35
BtsCI GGATG 1 cut(s) 241
BtsIMutI CAGTG 2 cut(s) 133, 276
Cac8I GCNNGC 4 cut(s) 274, 372, 376, 404
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 2 cut(s) 404, 406
Cfr10I RCCGGY 3 cut(s) 251, 266, 362
Cfr13I GGNCC 4 cut(s) 306, 376, 438, 495
Cfr42I CCGCGG 1 cut(s) 225
Csp6I GTAC 2 cut(s) 429, 473
CspAI ACCGGT 2 cut(s) 266, 362
CviAII CATG 4 cut(s) 76, 148, 208, 458
CviQI GTAC 2 cut(s) 429, 473
DpnI GATC 2 cut(s) 191, 419
DpnII GATC 2 cut(s) 189, 417
EaeI YGGCCR 2 cut(s) 153, 352
EagI CGGCCG 1 cut(s) 153
EclXI CGGCCG 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 207
Eco32I GATATC 1 cut(s) 339
Eco47I GGWCC 2 cut(s) 438, 495
Eco52I CGGCCG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 349
EcoRV GATATC 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 4 cut(s) 79, 151, 211, 461
FaiI YATR 8 cut(s) 53, 77, 149, 209, 258, 459, 483, 501
FaqI GGGAC 1 cut(s) 499
FatI CATG 4 cut(s) 75, 147, 207, 457
FauI CCCGC 4 cut(s) 2, 225, 367, 458
Fnu4HI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
GlaI GCGC 2 cut(s) 403, 405
GluI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
HaeIII GGCC 4 cut(s) 155, 308, 354, 378
HapII CCGG 4 cut(s) 156, 252, 267, 363
HhaI GCGC 2 cut(s) 404, 406
Hin1II CATG 4 cut(s) 79, 151, 211, 461
Hin6I GCGC 2 cut(s) 402, 404
HinP1I GCGC 2 cut(s) 402, 404
HinfI GANTC 1 cut(s) 26
HpaII CCGG 4 cut(s) 156, 252, 267, 363
HphI GGTGA 1 cut(s) 323
Hpy188I TCNGA 1 cut(s) 438
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 2 cut(s) 199, 290
HpyCH4V TGCA 2 cut(s) 4, 100
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 62, 269, 401
HpySE526I ACGT 2 cut(s) 199, 290
Hsp92II CATG 4 cut(s) 79, 151, 211, 461
HspAI GCGC 2 cut(s) 402, 404
KspI CCGCGG 1 cut(s) 225
Kzo9I GATC 2 cut(s) 189, 417
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 4 cut(s) 31, 53, 68, 328
LweI GCATC 1 cut(s) 172
MaeII ACGT 2 cut(s) 199, 290
MaeIII GTNAC 2 cut(s) 128, 382
MalI GATC 2 cut(s) 191, 419
MboI GATC 2 cut(s) 189, 417
MboII GAAGA 2 cut(s) 25, 127
MfeI CAATTG 1 cut(s) 135
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 2 cut(s) 135, 502
MluNI TGGCCA 1 cut(s) 354
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 3 cut(s) 70, 160, 382
Mox20I TGGCCA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 354
Msp20I TGGCCA 1 cut(s) 354
MspA1I CMGCKG 2 cut(s) 44, 224
MspI CCGG 4 cut(s) 156, 252, 267, 363
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 351
MvnI CGCG 3 cut(s) 20, 224, 404
MwoI GCNNNNNNNGC 4 cut(s) 53, 62, 269, 401
NcoI CCATGG 1 cut(s) 207
NdeII GATC 2 cut(s) 189, 417
NlaIII CATG 4 cut(s) 79, 151, 211, 461
NlaIV GGNNCC 4 cut(s) 283, 307, 361, 496
NmeAIII GCCGAG 2 cut(s) 208, 211
NmuCI GTSAC 2 cut(s) 128, 382
PauI GCGCGC 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PinAI ACCGGT 2 cut(s) 266, 362
PkrI GCNGC 7 cut(s) 43, 46, 58, 154, 223, 226, 318
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Ppu21I YACGTR 1 cut(s) 291
PpuMI RGGWCCY 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 4 cut(s) 283, 307, 361, 496
PspPI GGNCC 4 cut(s) 306, 376, 438, 495
PspPPI RGGWCCY 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 62
PteI GCGCGC 1 cut(s) 402
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 2 cut(s) 430, 474
RsaNI GTAC 2 cut(s) 429, 473
SacII CCGCGG 1 cut(s) 225
SatI GCNGC 7 cut(s) 42, 45, 57, 153, 222, 225, 317
Sau3AI GATC 2 cut(s) 189, 417
Sau96I GGNCC 4 cut(s) 306, 376, 438, 495
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 351
SetI ASST 7 cut(s) 46, 111, 202, 293, 352, 478, 500
SfaNI GCATC 1 cut(s) 172
Sfr303I CCGCGG 1 cut(s) 225
SgrBI CCGCGG 1 cut(s) 225
SinI GGWCC 2 cut(s) 438, 495
Sse9I AATT 2 cut(s) 135, 502
SsiI CCGC 9 cut(s) 9, 152, 222, 224, 232, 342, 374, 395, 465
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 2 cut(s) 202, 293
TaqI TCGA 2 cut(s) 29, 180
TasI AATT 2 cut(s) 135, 502
TauI GCSGC 3 cut(s) 155, 224, 227
TscAI CASTG 2 cut(s) 133, 283
TseFI GTSAC 2 cut(s) 128, 382
TseI GCWGC 4 cut(s) 41, 44, 56, 316
Tsp45I GTSAC 2 cut(s) 128, 382
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 133, 283
VpaK11BI GGWCC 2 cut(s) 438, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.