Rw3G020610

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013027
Physical Location & Seq
Reverse (-)
24257409 .. 24258733
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020610.1

Sequence Viewer

Length: 480 bp
ATGCAGTCAATCAAGGAAGCAACGGCCAACGTAGCTGCCTCTGCCAAGTCTGGCATGGAGAAGACCAAGGCCACCATGCAGGAGGAGATGGAGAAGATGAAGGCGCAGGACTCGATCCAAAAGGACATGGCAACTCAAAAGAAAGAAGGGCGGATTGCCGAGGCTGAGCAGCACAAGCGTGAAGCCATGGATCACAACACGGCCGCTAAGCATGGAGCAGCTCCGGCTGGATCCACATGCTATGGCACTAGGCCGACAGGATACAACACCAGCGACGAGGCTGGAACAGCCACATATCCAACCACTGGGGCTACTGGACAGCAAACCGGGTTCAATCAGATGTCCGCACTGCTCGGACATGGGACCGGGGAACCCTCTGGCCAGATAGGAATCAACACCGGCACCGGGATGACTACGGCCCATAACACCCATGTGGGTGGTAATGCCCCCGGCTATGGGACTGGCGGGACTTATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

159

Amino Acids

16.39

Weight (kDa)

6.58

Isoelectric Point (pI)

23.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 1.2e-17 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 401
AccB7I CCANNNNNTGG 1 cut(s) 305
AciI CCGC 4 cut(s) 151, 204, 345, 465
AclWI GGATC 4 cut(s) 109, 198, 225, 238
AcoI YGGCCR 3 cut(s) 24, 201, 379
AfiI CCNNNNNNNGG 4 cut(s) 305, 405, 455, 456
AgsI TTSAA 1 cut(s) 334
AleI CACNNNNGTG 2 cut(s) 177, 431
AluBI AGCT 2 cut(s) 35, 221
AluI AGCT 2 cut(s) 35, 221
AlwI GGATC 4 cut(s) 109, 198, 225, 238
AoxI GGCC 6 cut(s) 24, 69, 201, 251, 379, 417
ApeKI GCWGC 3 cut(s) 35, 169, 218
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 2 cut(s) 363, 418
AsuC2I CCSGG 4 cut(s) 328, 367, 406, 450
AvaII GGWCC 1 cut(s) 363
BalI TGGCCA 1 cut(s) 381
BamHI GGATCC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 22, 181, 230
BccI CCATC 1 cut(s) 82
BceAI ACGGC 3 cut(s) 39, 216, 432
BciVI GTATCC 1 cut(s) 254
BcnI CCSGG 4 cut(s) 328, 367, 406, 450
BfaI CTAG 1 cut(s) 249
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 4 cut(s) 36, 170, 204, 219
BlpI GCTNAGC 2 cut(s) 165, 207
BlsI GCNGC 4 cut(s) 37, 171, 205, 220
Bme1390I CCNGG 4 cut(s) 328, 367, 406, 450
Bme18I GGWCC 1 cut(s) 363
BmgT120I GGNCC 2 cut(s) 363, 418
BmiI GGNNCC 4 cut(s) 232, 364, 372, 403
BmrFI CCNGG 4 cut(s) 328, 367, 406, 450
BmrI ACTGGG 1 cut(s) 315
BmuI ACTGGG 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 68
Bpu1102I GCTNAGC 2 cut(s) 165, 207
BpuMI CCSGG 4 cut(s) 328, 367, 406, 450
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 5 cut(s) 66, 159, 186, 366, 448
Bsc4I CCNNNNNNNGG 4 cut(s) 305, 405, 455, 456
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 3 cut(s) 310, 319, 466
Bse8I GATNNNNATC 1 cut(s) 389
BseDI CCNNGG 5 cut(s) 66, 159, 186, 366, 448
BseGI GGATG 1 cut(s) 414
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 4 cut(s) 305, 405, 455, 456
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 3 cut(s) 310, 319, 466
BseRI GAGGAG 1 cut(s) 98
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 3 cut(s) 22, 181, 230
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 6 cut(s) 26, 71, 203, 253, 381, 419
BshNI GGYRCC 1 cut(s) 401
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 6 cut(s) 224, 327, 366, 399, 405, 450
BslFI GGGAC 2 cut(s) 376, 472
BslI CCNNNNNNNGG 4 cut(s) 305, 405, 455, 456
BsmFI GGGAC 2 cut(s) 376, 472
BsnI GGCC 6 cut(s) 26, 71, 203, 253, 381, 419
Bsp143I GATC 3 cut(s) 114, 190, 230
Bsp1720I GCTNAGC 2 cut(s) 165, 207
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 4 cut(s) 151, 204, 345, 465
BspANI GGCC 6 cut(s) 26, 71, 203, 253, 381, 419
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 4 cut(s) 232, 364, 372, 403
BspPI GGATC 4 cut(s) 109, 198, 225, 238
BspT107I GGYRCC 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 3 cut(s) 310, 319, 466
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 5 cut(s) 66, 159, 186, 366, 448
BssMI GATC 3 cut(s) 114, 190, 230
BssT1I CCWWGG 2 cut(s) 66, 186
BstDEI CTNAG 2 cut(s) 165, 207
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 414
BstHHI GCGC 1 cut(s) 106
BstKTI GATC 3 cut(s) 117, 193, 233
BstMBI GATC 3 cut(s) 114, 190, 230
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 287
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 4 cut(s) 326, 365, 404, 448
BstV1I GCAGC 3 cut(s) 22, 181, 230
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 437
BstYI RGATCY 1 cut(s) 230
BstZI CGGCCG 1 cut(s) 201
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 6 cut(s) 26, 71, 203, 253, 381, 419
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 347
BtsIMutI CAGTG 2 cut(s) 303, 347
CfoI GCGC 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 363, 418
CviAII CATG 8 cut(s) 55, 76, 127, 187, 212, 237, 359, 431
DdeI CTNAG 2 cut(s) 165, 207
DpnI GATC 3 cut(s) 116, 192, 232
DpnII GATC 3 cut(s) 114, 190, 230
EaeI YGGCCR 3 cut(s) 24, 201, 379
EagI CGGCCG 1 cut(s) 201
EciI GGCGGA 1 cut(s) 166
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 66, 186
Eco47I GGWCC 1 cut(s) 363
Eco52I CGGCCG 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 66, 186
ErhI CCWWGG 2 cut(s) 66, 186
FaeI CATG 8 cut(s) 58, 79, 130, 190, 215, 240, 362, 434
FaqI GGGAC 2 cut(s) 376, 472
FatI CATG 8 cut(s) 54, 75, 126, 186, 211, 236, 358, 430
FauI CCCGC 1 cut(s) 458
Fnu4HI GCNGC 4 cut(s) 36, 170, 204, 219
FokI GGATG 1 cut(s) 421
Fsp4HI GCNGC 4 cut(s) 36, 170, 204, 219
FspBI CTAG 1 cut(s) 249
GlaI GCGC 1 cut(s) 105
GluI GCNGC 4 cut(s) 36, 170, 204, 219
HaeIII GGCC 6 cut(s) 26, 71, 203, 253, 381, 419
HapII CCGG 6 cut(s) 224, 327, 366, 399, 405, 450
HhaI GCGC 1 cut(s) 106
Hin1II CATG 8 cut(s) 58, 79, 130, 190, 215, 240, 362, 434
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HinfI GANTC 2 cut(s) 110, 390
HpaII CCGG 6 cut(s) 224, 327, 366, 399, 405, 450
Hpy188I TCNGA 2 cut(s) 339, 356
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 94, 140
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 2 cut(s) 4, 79
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 287
HpyF3I CTNAG 2 cut(s) 165, 207
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 8 cut(s) 58, 79, 130, 190, 215, 240, 362, 434
HspAI GCGC 1 cut(s) 104
Kzo9I GATC 3 cut(s) 114, 190, 230
LmnI GCTCC 2 cut(s) 215, 226
Lsp1109I GCAGC 3 cut(s) 22, 181, 230
MaeI CTAG 1 cut(s) 249
MaeII ACGT 1 cut(s) 30
MalI GATC 3 cut(s) 116, 192, 232
MboI GATC 3 cut(s) 114, 190, 230
MboII GAAGA 2 cut(s) 73, 106
MflI RGATCY 1 cut(s) 230
MlsI TGGCCA 1 cut(s) 381
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 323
MnlI CCTC 5 cut(s) 49, 76, 154, 271, 385
Mox20I TGGCCA 1 cut(s) 381
MscI TGGCCA 1 cut(s) 381
MslI CAYNNNNRTG 4 cut(s) 177, 407, 431, 435
Msp20I TGGCCA 1 cut(s) 381
MspI CCGG 6 cut(s) 224, 327, 366, 399, 405, 450
MspR9I CCNGG 4 cut(s) 328, 367, 406, 450
MwoI GCNNNNNNNGC 5 cut(s) 32, 41, 175, 224, 287
NciI CCSGG 4 cut(s) 328, 367, 406, 450
NcoI CCATGG 1 cut(s) 186
NdeII GATC 3 cut(s) 114, 190, 230
NlaIII CATG 8 cut(s) 58, 79, 130, 190, 215, 240, 362, 434
NlaIV GGNNCC 4 cut(s) 232, 364, 372, 403
NmeAIII GCCGAG 1 cut(s) 184
NspI RCATGY 1 cut(s) 240
OliI CACNNNNGTG 2 cut(s) 177, 431
PfeI GAWTC 1 cut(s) 390
PflMI CCANNNNNTGG 1 cut(s) 305
PkrI GCNGC 4 cut(s) 37, 171, 205, 220
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PspN4I GGNNCC 4 cut(s) 232, 364, 372, 403
PspPI GGNCC 2 cut(s) 363, 418
PsuI RGATCY 1 cut(s) 230
RseI CAYNNNNRTG 4 cut(s) 177, 407, 431, 435
SatI GCNGC 4 cut(s) 36, 170, 204, 219
Sau3AI GATC 3 cut(s) 114, 190, 230
Sau96I GGNCC 2 cut(s) 363, 418
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 4 cut(s) 328, 367, 406, 450
SetI ASST 3 cut(s) 33, 37, 223
SinI GGWCC 1 cut(s) 363
SmiMI CAYNNNNRTG 4 cut(s) 177, 407, 431, 435
SsiI CCGC 4 cut(s) 151, 204, 345, 465
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 4 cut(s) 326, 365, 404, 448
StyI CCWWGG 2 cut(s) 66, 186
TaiI ACGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 113
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 1 cut(s) 390
TscAI CASTG 2 cut(s) 310, 354
TseI GCWGC 3 cut(s) 35, 169, 218
TspDTI ATGAA 1 cut(s) 113
TspRI CASTG 2 cut(s) 310, 354
Van91I CCANNNNNTGG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 363
XceI RCATGY 1 cut(s) 240
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.