Rorug03G0178400

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013027
Physical Location & Seq
Forward (+)
15169056 .. 15173368
4313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0178400.1

Sequence Viewer

Length: 1908 bp
ATGGCGGTACCGCAGACTCCGGCGACGGCGCTCACCGTGATGGCGTCTCTGTACGTCGGAGACCTCAGCCCCTACATCACCGAGACCGAACTCTACGAGGCGTTCGAAAACGAGAAAAAGACCCTTGCGTCCGTTAGGGTGTGCAGGGACACCAACACCGGCCGGTCTCTCTGCTACGGCTACGCCAACTTTTCTAACACCGACGACGCAATTCGAGCCATGGAACAGAAGAATAACTCTGTTCTGCATGGAAGAGTGATAAGGGTCATGTGGTCTCATCGTGATCCTGATGCCAGGAGAAGTGGAATTGGGAATGTGTTTGTCAAGAACTTGAGCGAAGGGATTGATAATGTAGGGCTCCACGACATGTTTAGGAAATTTGGAAATGTTTTGTCTTGCAAAGTTGCCATGTTTGAGGATGGGAAGAGTAAAGGCTATGGCTTTGTTCAGTTTGAGTCTGAGGAATCAGCAATTGCTGCTATTGACAAGCTAAATGGCTCTACAGTCGGAGACAAACAATTGTATGTCGCTAAGTTTGTTAGAAAGAGTGATCGAGTTTTGCCCAATCCTGATGTGAAATATACAAATTTGTATGTGAAGAATTTGGATCCAAATGTCAGGGAAGAGCTTCTGGAAGAAAAGTTCTCTGAGTTCGGGAAAATTATAAGCTTTGCAATAGCAAGGGATGAAAATGGAATTTCTAGAGGTTTTGGCTTTGTGAACTTTGAGAGCCCGGATGATGCTAGAAAAGCATTGGAATCTCTGAATGGAACTCAACTTGGTTCCAAGGTTCTGTATGTAGCGAGAGCACAGAAGAAGGCAGAGCGTGAACACATTTTGCGTCGTCAGTTTGAGGAAAAACGGAAGGAGCAAATGTTAAAATTCAAGGGATCAAACGTGTATGTGAAGAACATTGATGATGATGTCACTGATGAAGAGCTCATAGACCACTTCAGTCAATGTGGCACGATTACTTCTGCAAAAATTATGAGAGATGAGAAAGGAATAAGCAAGGGCTTTGGTTTTGTTTGCTTCTCTACCCCCGAGGAGGGCAATAAAGCTGTGATTACATTTCATGGATATATGTTTCATCGGAAGCCATTGTATGTCGCTATTGCTCAAAGGAAAGAAGAAAGACAAGCACAGCTGCAACTTCAGTATGCACAGCGTATGGCAGGATTTGCTGGTCCTTCAACAGCTGTCATCCCTGGTGGATACCCTCCTATCTACTATCAGGCTCCATCTGGTGTTGTTCCACCAGTGCCTCCTCGACCAGGGATGATGTATCAGCCTTTGGGATTGAGGCCAGGATGGAGACCTAATGGCTTTGCACCTACCTCTGGACCAGGTTATCAACCACCACCTGTTCCTGTTATGTCCAATACTCCAAGGCAGCATAGGCAAAACAGGGGCAGAATAAATGGGCATATGCTTCCTCAGGGAGGTCCATTTATTCCACATTTGCAACAGTCAACTCACTCCTCGAAGGAGTCAAACAATCAGCAGCGTTCGGGGCAGCCTAAGTATGTTCCTAATGGGCGTCAACGTGACATGAATAAAGGATCAGGAGTCTCATCTACTGCTTCCAATTCTGTTGGAGGTGGGTCTCAGGGACCAGAGATGCTGAGTAGTATGCTTGCTGCTGCTTCTCCTGAGCAGCAGAAACAGATACTCGGCGAGCGTCTCTTCCCACTAGTTCATAAACACAAGCCGGATCTTGCTTCAAAAATTACCGGGATGCTGCTGGAGATGGACAACTCAGAACTGCTGCTTTTGTTGGAGTCTCCTGAGAGTTTGGCTGCCAAAGTGGGAGAAGCTGTTCAGGTCCTCAAGATCTCCAAGACTAAAGTATCTAGCAAAGATTCCCTTCATTCTAGTTACCTTTCTGCTGAGGTTGCAGTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

635

Amino Acids

70.28

Weight (kDa)

9.16

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 17 - 88 8.8e-14 RNA recognition motif
RRM_1 PF00076 106 - 174 4.2e-20 RNA recognition motif
RRM_1 PF00076 197 - 265 1e-22 RNA recognition motif
RRM_1 PF00076 300 - 368 8.3e-17 RNA recognition motif
MLLE PF00658 545 - 610 4.3e-30 MLLE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 665
AasI GACNNNNNNGTC 1 cut(s) 127
Acc65I GGTACC 1 cut(s) 7
AccB1I GGYRCC 1 cut(s) 7
AciI CCGC 2 cut(s) 5, 11
AclWI GGATC 6 cut(s) 278, 602, 615, 898, 1570, 1722
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 5 cut(s) 377, 586, 601, 696, 881
AcuI CTGAAG 2 cut(s) 937, 1139
AcyI GRCGYC 2 cut(s) 44, 1540
AfaI GTAC 2 cut(s) 9, 53
AfiI CCNNNNNNNGG 5 cut(s) 1048, 1049, 1274, 1340, 1442
AflIII ACRYGT 2 cut(s) 366, 897
AgsI TTSAA 3 cut(s) 886, 1194, 1725
AhlI ACTAGT 1 cut(s) 1693
AjnI CCWGG 5 cut(s) 293, 1207, 1273, 1306, 1345
AloI GAACNNNNNNTCC 4 cut(s) 626, 658, 1803, 1835
AluBI AGCT 8 cut(s) 490, 628, 669, 940, 1061, 1147, 1199, 1817
AluI AGCT 8 cut(s) 490, 628, 669, 940, 1061, 1147, 1199, 1817
Alw21I GWGCWC 2 cut(s) 811, 942
AlwI GGATC 6 cut(s) 278, 602, 615, 898, 1570, 1722
Ama87I CYCGRG 1 cut(s) 1043
AoxI GGCC 2 cut(s) 160, 1304
ApoI RAATTY 5 cut(s) 377, 586, 601, 696, 881
Asp700I GAANNNNTTC 2 cut(s) 627, 1818
Asp718I GGTACC 1 cut(s) 7
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
AsuC2I CCSGG 2 cut(s) 734, 1735
AsuHPI GGTGA 2 cut(s) 25, 70
AsuII TTCGAA 1 cut(s) 105
AvaI CYCGRG 1 cut(s) 1043
AvaII GGWCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
AxyI CCTNAGG 1 cut(s) 1437
BaeI ACNNNNGTAYC 2 cut(s) 1833, 1866
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 3 cut(s) 360, 734, 942
Bbv12I GWGCWC 2 cut(s) 811, 942
BbvCI CCTCAGC 2 cut(s) 65, 1890
BccI CCATC 5 cut(s) 34, 413, 1249, 1305, 1744
BceAI ACGGC 2 cut(s) 42, 193
BciT130I CCWGG 5 cut(s) 295, 1209, 1275, 1308, 1347
BciVI GTATCC 1 cut(s) 1208
BcnI CCSGG 2 cut(s) 734, 1735
BcuI ACTAGT 1 cut(s) 1693
BfaI CTAG 5 cut(s) 702, 744, 1694, 1854, 1875
BfmI CTRYAG 1 cut(s) 501
BfoI RGCGCY 1 cut(s) 32
BfuI GTATCC 1 cut(s) 1208
BglII AGATCT 1 cut(s) 1833
Bme1390I CCNGG 7 cut(s) 295, 734, 1209, 1275, 1308, 1347, 1735
Bme18I GGWCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
BmeT110I CYCGRG 1 cut(s) 1043
BmgT120I GGNCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
BmiI GGNNCC 6 cut(s) 9, 359, 609, 784, 1239, 1614
BmrFI CCNGG 7 cut(s) 295, 734, 1209, 1275, 1308, 1347, 1735
BmsI GCATC 4 cut(s) 280, 730, 1611, 1728
BpmI CTGGAG 1 cut(s) 1766
Bpu10I CCTNAGC 3 cut(s) 65, 1653, 1890
Bpu14I TTCGAA 1 cut(s) 105
BpuEI CTTGAG 2 cut(s) 352, 1814
BpuMI CCSGG 2 cut(s) 734, 1735
BsaHI GRCGYC 2 cut(s) 44, 1540
BsaI GGTCTC 6 cut(s) 54, 77, 171, 279, 1309, 1611
BsaJI CCNNGG 6 cut(s) 219, 786, 1044, 1207, 1274, 1388
BsaXI ACNNNNNCTCC 4 cut(s) 860, 890, 1803, 1833
Bsc4I CCNNNNNNNGG 5 cut(s) 1048, 1049, 1274, 1340, 1442
Bse118I RCCGGY 2 cut(s) 158, 162
Bse1I ACTGG 1 cut(s) 1259
Bse21I CCTNAGG 1 cut(s) 1437
BseBI CCWGG 5 cut(s) 295, 1209, 1275, 1308, 1347
BseDI CCNNGG 6 cut(s) 219, 786, 1044, 1207, 1274, 1388
BseGI GGATG 7 cut(s) 424, 691, 742, 1203, 1284, 1316, 1743
BseLI CCNNNNNNNGG 5 cut(s) 1048, 1049, 1274, 1340, 1442
BseNI ACTGG 1 cut(s) 1259
BseRI GAGGAG 3 cut(s) 1061, 1257, 1471
BseX3I CGGCCG 1 cut(s) 160
BsgI GTGCAG 1 cut(s) 163
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 2 cut(s) 162, 1306
BshNI GGYRCC 1 cut(s) 7
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 2 cut(s) 811, 942
BsiHKCI CYCGRG 1 cut(s) 1043
BsiSI CCGG 6 cut(s) 20, 159, 163, 734, 1712, 1734
BslFI GGGAC 2 cut(s) 161, 1626
BslI CCNNNNNNNGG 5 cut(s) 1048, 1049, 1274, 1340, 1442
BsmBI CGTCTC 2 cut(s) 51, 1688
BsmFI GGGAC 2 cut(s) 161, 1626
BsnI GGCC 2 cut(s) 162, 1306
Bso31I GGTCTC 6 cut(s) 54, 77, 171, 279, 1309, 1611
BsoBI CYCGRG 1 cut(s) 1043
Bsp119I TTCGAA 1 cut(s) 105
Bsp1286I GDGCHC 4 cut(s) 360, 734, 811, 942
Bsp143I GATC 7 cut(s) 283, 550, 607, 890, 1562, 1714, 1833
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 2 cut(s) 5, 11
BspANI GGCC 2 cut(s) 162, 1306
BspLI GGNNCC 6 cut(s) 9, 359, 609, 784, 1239, 1614
BspPI GGATC 6 cut(s) 278, 602, 615, 898, 1570, 1722
BspQI GCTCTTC 2 cut(s) 618, 930
BspT104I TTCGAA 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 7
BspTNI GGTCTC 6 cut(s) 54, 77, 171, 279, 1309, 1611
BsrFI RCCGGY 2 cut(s) 158, 162
BsrI ACTGG 1 cut(s) 1259
BssAI RCCGGY 2 cut(s) 158, 162
BssECI CCNNGG 6 cut(s) 219, 786, 1044, 1207, 1274, 1388
BssMI GATC 7 cut(s) 283, 550, 607, 890, 1562, 1714, 1833
BssNI GRCGYC 2 cut(s) 44, 1540
BssT1I CCWWGG 3 cut(s) 219, 786, 1388
Bst2UI CCWGG 5 cut(s) 295, 1209, 1275, 1308, 1347
Bst4CI ACNGT 3 cut(s) 37, 505, 1470
Bst6I CTCTTC 5 cut(s) 247, 419, 618, 930, 1691
BstACI GRCGYC 2 cut(s) 44, 1540
BstAPI GCANNNNNTGC 2 cut(s) 476, 1181
BstBI TTCGAA 1 cut(s) 105
BstC8I GCNNGC 2 cut(s) 1638, 1679
BstDSI CCRYGG 1 cut(s) 219
BstENI CCTNNNNNAGG 2 cut(s) 1272, 1440
BstF5I GGATG 7 cut(s) 424, 691, 742, 1203, 1284, 1316, 1743
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 7 cut(s) 286, 553, 610, 893, 1565, 1717, 1836
BstMBI GATC 7 cut(s) 283, 550, 607, 890, 1562, 1714, 1833
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 7 cut(s) 215, 476, 749, 1181, 1399, 1513, 1895
BstNI CCWGG 5 cut(s) 295, 1209, 1275, 1308, 1347
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 7 cut(s) 293, 732, 1207, 1273, 1306, 1345, 1733
BstSFI CTRYAG 1 cut(s) 501
BstX2I RGATCY 3 cut(s) 607, 1714, 1833
BstYI RGATCY 3 cut(s) 607, 1714, 1833
BstZI CGGCCG 1 cut(s) 160
Bsu36I CCTNAGG 1 cut(s) 1437
BsuI GTATCC 1 cut(s) 1208
BsuRI GGCC 2 cut(s) 162, 1306
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 7 cut(s) 424, 691, 742, 1203, 1284, 1316, 1743
BtsIMutI CAGTG 2 cut(s) 927, 1266
Cac8I GCNNGC 2 cut(s) 1638, 1679
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 2 cut(s) 158, 162
Cfr13I GGNCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
CseI GACGC 6 cut(s) 33, 117, 215, 830, 1529, 1670
CsiI ACCWGGT 1 cut(s) 1345
Csp6I GTAC 2 cut(s) 8, 52
CviAII CATG 7 cut(s) 220, 248, 268, 367, 409, 1076, 1552
CviQI GTAC 2 cut(s) 8, 52
DpnI GATC 7 cut(s) 285, 552, 609, 892, 1564, 1716, 1835
DpnII GATC 7 cut(s) 283, 550, 607, 890, 1562, 1714, 1833
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 160
EagI CGGCCG 1 cut(s) 160
Eam1104I CTCTTC 5 cut(s) 247, 419, 618, 930, 1691
EarI CTCTTC 5 cut(s) 247, 419, 618, 930, 1691
Ecl136II GAGCTC 1 cut(s) 940
EclXI CGGCCG 1 cut(s) 160
Eco130I CCWWGG 3 cut(s) 219, 786, 1388
Eco24I GRGCYC 3 cut(s) 360, 734, 942
Eco31I GGTCTC 6 cut(s) 54, 77, 171, 279, 1309, 1611
Eco47I GGWCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
Eco52I CGGCCG 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 940
Eco57I CTGAAG 2 cut(s) 937, 1139
Eco81I CCTNAGG 1 cut(s) 1437
Eco88I CYCGRG 1 cut(s) 1043
EcoICRI GAGCTC 1 cut(s) 940
EcoNI CCTNNNNNAGG 2 cut(s) 1272, 1440
EcoO109I RGGNCCY 1 cut(s) 1825
EcoRII CCWGG 5 cut(s) 293, 1207, 1273, 1306, 1345
EcoT14I CCWWGG 3 cut(s) 219, 786, 1388
EcoT38I GRGCYC 3 cut(s) 360, 734, 942
ErhI CCWWGG 3 cut(s) 219, 786, 1388
Esp3I CGTCTC 2 cut(s) 51, 1688
FaeI CATG 7 cut(s) 223, 251, 271, 370, 412, 1079, 1555
FalI AAGNNNNNCTT 2 cut(s) 1851, 1883
FaqI GGGAC 2 cut(s) 161, 1626
FatI CATG 7 cut(s) 219, 247, 267, 366, 408, 1075, 1551
FauNDI CATATG 1 cut(s) 1428
FokI GGATG 7 cut(s) 431, 698, 749, 1190, 1291, 1323, 1750
FriOI GRGCYC 3 cut(s) 360, 734, 942
FspBI CTAG 5 cut(s) 702, 744, 1694, 1854, 1875
GlaI GCGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 1766
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 162, 1306
HapII CCGG 6 cut(s) 20, 159, 163, 734, 1712, 1734
HgaI GACGC 6 cut(s) 33, 117, 215, 830, 1529, 1670
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 2 cut(s) 44, 1540
Hin1II CATG 7 cut(s) 223, 251, 271, 370, 412, 1079, 1555
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 2 cut(s) 1473, 1544
HindII GTYRAC 2 cut(s) 1473, 1544
HindIII AAGCTT 1 cut(s) 667
HinfI GANTC 8 cut(s) 16, 455, 464, 758, 1490, 1569, 1781, 1862
HpaII CCGG 6 cut(s) 20, 159, 163, 734, 1712, 1734
HphI GGTGA 2 cut(s) 25, 70
Hpy166II GTNNAC 4 cut(s) 721, 830, 1473, 1544
Hpy188I TCNGA 7 cut(s) 59, 460, 509, 649, 765, 1095, 1762
Hpy8I GTNNAC 4 cut(s) 721, 830, 1473, 1544
Hpy99I CGWCG 5 cut(s) 28, 59, 206, 209, 846
HpyAV CCTTC 6 cut(s) 332, 811, 859, 1200, 1480, 1877
HpyCH4III ACNGT 3 cut(s) 37, 505, 1470
HpyCH4IV ACGT 3 cut(s) 54, 897, 1546
HpyF10VI GCNNNNNNNGC 7 cut(s) 215, 476, 749, 1181, 1399, 1513, 1895
HpySE526I ACGT 3 cut(s) 54, 897, 1546
Hsp92I GRCGYC 2 cut(s) 44, 1540
Hsp92II CATG 7 cut(s) 223, 251, 271, 370, 412, 1079, 1555
HspAI GCGC 1 cut(s) 29
KpnI GGTACC 1 cut(s) 11
Kzo9I GATC 7 cut(s) 283, 550, 607, 890, 1562, 1714, 1833
LguI GCTCTTC 2 cut(s) 618, 930
LmnI GCTCC 3 cut(s) 363, 868, 1243
LweI GCATC 4 cut(s) 280, 730, 1611, 1728
MabI ACCWGGT 1 cut(s) 1345
MaeI CTAG 5 cut(s) 702, 744, 1694, 1854, 1875
MaeII ACGT 3 cut(s) 54, 897, 1546
MaeIII GTNAC 3 cut(s) 925, 1547, 1877
MalI GATC 7 cut(s) 285, 552, 609, 892, 1564, 1716, 1835
MboI GATC 7 cut(s) 283, 550, 607, 890, 1562, 1714, 1833
MfeI CAATTG 3 cut(s) 471, 518, 1903
MflI RGATCY 3 cut(s) 607, 1714, 1833
MhlI GDGCHC 4 cut(s) 360, 734, 811, 942
MlyI GAGTC 5 cut(s) 10, 464, 1499, 1578, 1790
MmeI TCCRAC 4 cut(s) 37, 487, 1576, 1758
MroXI GAANNNNTTC 2 cut(s) 627, 1818
MseI TTAA 1 cut(s) 878
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 2 cut(s) 1147, 1199
MspI CCGG 6 cut(s) 20, 159, 163, 734, 1712, 1734
MspR9I CCNGG 7 cut(s) 295, 734, 1209, 1275, 1308, 1347, 1735
MunI CAATTG 3 cut(s) 471, 518, 1903
MvaI CCWGG 5 cut(s) 295, 1209, 1275, 1308, 1347
MwoI GCNNNNNNNGC 7 cut(s) 215, 476, 749, 1181, 1399, 1513, 1895
NciI CCSGG 2 cut(s) 734, 1735
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 1428
NdeII GATC 7 cut(s) 283, 550, 607, 890, 1562, 1714, 1833
NlaIII CATG 7 cut(s) 223, 251, 271, 370, 412, 1079, 1555
NlaIV GGNNCC 6 cut(s) 9, 359, 609, 784, 1239, 1614
NmeAIII GCCGAG 1 cut(s) 1653
NmuCI GTSAC 2 cut(s) 925, 1547
NspI RCATGY 1 cut(s) 370
NspV TTCGAA 1 cut(s) 105
PciI ACATGT 1 cut(s) 366
PciSI GCTCTTC 2 cut(s) 618, 930
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 2 cut(s) 627, 1818
PfeI GAWTC 3 cut(s) 464, 758, 1862
PleI GAGTC 5 cut(s) 10, 463, 1498, 1577, 1789
PpsI GAGTC 5 cut(s) 10, 463, 1498, 1577, 1789
PpuMI RGGWCCY 1 cut(s) 1825
PscI ACATGT 1 cut(s) 366
PsiI TTATAA 1 cut(s) 665
Psp124BI GAGCTC 1 cut(s) 942
Psp5II RGGWCCY 1 cut(s) 1825
Psp6I CCWGG 5 cut(s) 293, 1207, 1273, 1306, 1345
PspGI CCWGG 5 cut(s) 293, 1207, 1273, 1306, 1345
PspN4I GGNNCC 6 cut(s) 9, 359, 609, 784, 1239, 1614
PspPI GGNCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
PspPPI RGGWCCY 1 cut(s) 1825
PsuI RGATCY 3 cut(s) 607, 1714, 1833
PvuII CAGCTG 2 cut(s) 1147, 1199
RsaI GTAC 2 cut(s) 9, 53
RsaNI GTAC 2 cut(s) 8, 52
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 942
SapI GCTCTTC 2 cut(s) 618, 930
SaqAI TTAA 1 cut(s) 878
Sau3AI GATC 7 cut(s) 283, 550, 607, 890, 1562, 1714, 1833
Sau96I GGNCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
SchI GAGTC 5 cut(s) 10, 464, 1499, 1578, 1790
ScrFI CCNGG 7 cut(s) 295, 734, 1209, 1275, 1308, 1347, 1735
SduI GDGCHC 4 cut(s) 360, 734, 811, 942
SexAI ACCWGGT 1 cut(s) 1345
SfaNI GCATC 4 cut(s) 280, 730, 1611, 1728
SfcI CTRYAG 1 cut(s) 501
SfuI TTCGAA 1 cut(s) 105
SinI GGWCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 331, 1829
SmoI CTYRAG 2 cut(s) 331, 1829
SpeI ACTAGT 1 cut(s) 1693
SsiI CCGC 2 cut(s) 5, 11
SspMI CTAG 5 cut(s) 702, 744, 1694, 1854, 1875
SstI GAGCTC 1 cut(s) 942
StyD4I CCNGG 7 cut(s) 293, 732, 1207, 1273, 1306, 1345, 1733
StyI CCWWGG 3 cut(s) 219, 786, 1388
TaaI ACNGT 3 cut(s) 37, 505, 1470
TaiI ACGT 3 cut(s) 57, 900, 1549
TaqI TCGA 5 cut(s) 105, 214, 553, 1270, 1484
TaqII GACCGA 1 cut(s) 101
TfiI GAWTC 3 cut(s) 464, 758, 1862
Tru1I TTAA 1 cut(s) 878
Tru9I TTAA 1 cut(s) 878
TscAI CASTG 2 cut(s) 934, 1266
TseFI GTSAC 2 cut(s) 925, 1547
Tsp45I GTSAC 2 cut(s) 925, 1547
TspDTI ATGAA 7 cut(s) 702, 948, 1064, 1079, 1568, 1688, 1859
TspGWI ACGGA 2 cut(s) 121, 877
TspRI CASTG 2 cut(s) 934, 1266
VpaK11BI GGWCC 5 cut(s) 1187, 1343, 1445, 1613, 1825
XagI CCTNNNNNAGG 2 cut(s) 1272, 1440
XapI RAATTY 5 cut(s) 377, 586, 601, 696, 881
XbaI TCTAGA 1 cut(s) 701
XceI RCATGY 1 cut(s) 370
XmnI GAANNNNTTC 2 cut(s) 627, 1818
XspI CTAG 5 cut(s) 702, 744, 1694, 1854, 1875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.