Rw0G015300

18 kDa seed maturation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00692
OG0013027
Physical Location & Seq
Reverse (-)
94704 .. 95536
833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015300.1

Sequence Viewer

Length: 507 bp
ATGCAGTCAATCAAGGAAGCCGCGGCCAACGTAGCTGCCTCTGCCAAGTCTGGCATGGAGAAGACCAAGGCCACCATGCAGGAGAAGATGGAGAAGATGAAGGCACAGGACCCGATCCAAAAGGACATGGCAACTCAAAAGAAAGAAGGGCGGATTGCCGAGGCTGAGCAGCACAAGCGTGAGGCCATGGATCACAACACGGCCGCTAAGCATGGAGCAGCTCCAGCTGGATCCACAGGCTACGGCACTGGGCCGACAGGATACAGCACCAGCGGGACTGGAACAGCCACATATCCAACCACCGGGGCTACTGGGCAGCAAACCGGGTTCAATCAGATGTCCGCGCTGCCCGGACATGGGACCGAGGAACCCGCTGGCCAAGTGGTTGAGGGTTTTGTCACCGCACATCCGATCGGAACCAACACCGGCACCGGGAGGACTACGGACCATAACACCCATGTGGGTGGTAATGCCCCCGGCTATGGGACTGGCGGGACTTATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0030246 GO:0031210 GO:0043167 GO:0043168 GO:0043169 GO:0048030 GO:0050997 GO:0070405 GO:0070492
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

168

Amino Acids

17.17

Weight (kDa)

7.07

Isoelectric Point (pI)

21.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 7.9e-20 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06760
fragaria_vesca FvH4_6g22410
malus_domestica MD12G1057800.v1.1
prunus_persica Prupe.7G076400_v2.0.a1 Prupe.7G076400_v2.0.a1
pyrus_communis pycom12g05130 pycom14g04720
rosa_chinensis RchiOBHm_Chr3g0479771
rosa_laevigata RLG00000023541
rosa_multiflora Rmu_sc0004329.1_g000013
rosa_roxburghii Rroxscaffold_6G00401990
rosa_rugosa Rorug03G0178400
rosa_samantha Rh3AG229100 Rh3BG262300 Rh3CG259000 Rh3DG255300
rosa_wichuraiana Rw0G015300 Rw3G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 428
AccII CGCG 2 cut(s) 23, 344
AciI CCGC 9 cut(s) 21, 23, 151, 204, 273, 342, 372, 402, 492
AclWI GGATC 4 cut(s) 109, 198, 225, 238
AcoI YGGCCR 3 cut(s) 24, 201, 376
AfiI CCNNNNNNNGG 6 cut(s) 302, 356, 357, 432, 482, 483
AgsI TTSAA 1 cut(s) 331
AleI CACNNNNGTG 2 cut(s) 177, 458
AluBI AGCT 3 cut(s) 35, 221, 227
AluI AGCT 3 cut(s) 35, 221, 227
AlwI GGATC 4 cut(s) 109, 198, 225, 238
AoxI GGCC 6 cut(s) 24, 69, 183, 201, 251, 376
ApeKI GCWGC 5 cut(s) 35, 169, 218, 316, 346
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 4 cut(s) 109, 251, 360, 445
AsuC2I CCSGG 5 cut(s) 304, 325, 351, 433, 477
AsuHPI GGTGA 1 cut(s) 391
AvaII GGWCC 3 cut(s) 109, 360, 445
BalI TGGCCA 1 cut(s) 378
BamHI GGATCC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 5 cut(s) 22, 181, 230, 328, 333
BccI CCATC 1 cut(s) 82
BceAI ACGGC 2 cut(s) 216, 259
BciVI GTATCC 1 cut(s) 254
BcnI CCSGG 5 cut(s) 304, 325, 351, 433, 477
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 8 cut(s) 21, 24, 36, 170, 204, 219, 317, 347
BlpI GCTNAGC 2 cut(s) 165, 207
BlsI GCNGC 8 cut(s) 22, 25, 37, 171, 205, 220, 318, 348
Bme1390I CCNGG 5 cut(s) 304, 325, 351, 433, 477
Bme18I GGWCC 3 cut(s) 109, 360, 445
BmgT120I GGNCC 4 cut(s) 109, 251, 360, 445
BmiI GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
BmrFI CCNGG 5 cut(s) 304, 325, 351, 433, 477
BmrI ACTGGG 2 cut(s) 258, 321
BmuI ACTGGG 2 cut(s) 258, 321
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 207
Bpu1102I GCTNAGC 2 cut(s) 165, 207
BpuMI CCSGG 5 cut(s) 304, 325, 351, 433, 477
BsaJI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
Bsc4I CCNNNNNNNGG 6 cut(s) 302, 356, 357, 432, 482, 483
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 4 cut(s) 253, 283, 316, 493
BseDI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 6 cut(s) 302, 356, 357, 432, 482, 483
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 4 cut(s) 253, 283, 316, 493
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 5 cut(s) 22, 181, 230, 328, 333
Bsh1236I CGCG 2 cut(s) 23, 344
Bsh1285I CGRYCG 2 cut(s) 204, 414
BshFI GGCC 6 cut(s) 26, 71, 185, 203, 253, 378
BshNI GGYRCC 1 cut(s) 428
BsiEI CGRYCG 2 cut(s) 204, 414
BsiSI CCGG 6 cut(s) 303, 324, 351, 426, 432, 477
BslFI GGGAC 3 cut(s) 289, 373, 499
BslI CCNNNNNNNGG 6 cut(s) 302, 356, 357, 432, 482, 483
BsmFI GGGAC 3 cut(s) 289, 373, 499
BsnI GGCC 6 cut(s) 26, 71, 185, 203, 253, 378
Bsp143I GATC 4 cut(s) 114, 190, 230, 411
Bsp1720I GCTNAGC 2 cut(s) 165, 207
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 9 cut(s) 21, 23, 151, 204, 273, 342, 372, 402, 492
BspANI GGCC 6 cut(s) 26, 71, 185, 203, 253, 378
BspCNI CTCAG 1 cut(s) 157
BspFNI CGCG 2 cut(s) 23, 344
BspLI GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
BspPI GGATC 4 cut(s) 109, 198, 225, 238
BspT107I GGYRCC 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 4 cut(s) 253, 283, 316, 493
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 7 cut(s) 21, 66, 159, 186, 303, 363, 475
BssMI GATC 4 cut(s) 114, 190, 230, 411
BssT1I CCWWGG 2 cut(s) 66, 186
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 2 cut(s) 165, 207
BstDSI CCRYGG 2 cut(s) 21, 186
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 2 cut(s) 23, 344
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 4 cut(s) 117, 193, 233, 414
BstMBI GATC 4 cut(s) 114, 190, 230, 411
BstMCI CGRYCG 2 cut(s) 204, 414
BstMWI GCNNNNNNNGC 4 cut(s) 32, 41, 175, 224
BstSCI CCNGG 5 cut(s) 302, 323, 349, 431, 475
BstUI CGCG 2 cut(s) 23, 344
BstV1I GCAGC 5 cut(s) 22, 181, 230, 328, 333
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 1 cut(s) 230
BstZI CGGCCG 1 cut(s) 201
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 6 cut(s) 26, 71, 185, 203, 253, 378
BtgI CCRYGG 2 cut(s) 21, 186
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 376
CfoI GCGC 1 cut(s) 346
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 4 cut(s) 109, 251, 360, 445
Cfr42I CCGCGG 1 cut(s) 24
CviAII CATG 7 cut(s) 55, 76, 127, 187, 212, 356, 458
DdeI CTNAG 2 cut(s) 165, 207
DpnI GATC 4 cut(s) 116, 192, 232, 413
DpnII GATC 4 cut(s) 114, 190, 230, 411
EaeI YGGCCR 3 cut(s) 24, 201, 376
EagI CGGCCG 1 cut(s) 201
EciI GGCGGA 1 cut(s) 166
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 66, 186
Eco47I GGWCC 3 cut(s) 109, 360, 445
Eco52I CGGCCG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 66, 186
ErhI CCWWGG 2 cut(s) 66, 186
FaeI CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
FaqI GGGAC 3 cut(s) 289, 373, 499
FatI CATG 7 cut(s) 54, 75, 126, 186, 211, 355, 457
FauI CCCGC 3 cut(s) 266, 379, 485
Fnu4HI GCNGC 8 cut(s) 21, 24, 36, 170, 204, 219, 317, 347
FokI GGATG 1 cut(s) 393
Fsp4HI GCNGC 8 cut(s) 21, 24, 36, 170, 204, 219, 317, 347
GlaI GCGC 1 cut(s) 345
GluI GCNGC 8 cut(s) 21, 24, 36, 170, 204, 219, 317, 347
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 6 cut(s) 26, 71, 185, 203, 253, 378
HapII CCGG 6 cut(s) 303, 324, 351, 426, 432, 477
HhaI GCGC 1 cut(s) 346
Hin1II CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HpaII CCGG 6 cut(s) 303, 324, 351, 426, 432, 477
HphI GGTGA 1 cut(s) 391
Hpy188I TCNGA 3 cut(s) 336, 411, 416
HpyAV CCTTC 2 cut(s) 94, 140
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 2 cut(s) 4, 79
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 41, 175, 224
HpyF3I CTNAG 2 cut(s) 165, 207
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
HspAI GCGC 1 cut(s) 344
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 4 cut(s) 114, 190, 230, 411
LmnI GCTCC 2 cut(s) 215, 226
Lsp1109I GCAGC 5 cut(s) 22, 181, 230, 328, 333
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 397
MalI GATC 4 cut(s) 116, 192, 232, 413
MboI GATC 4 cut(s) 114, 190, 230, 411
MboII GAAGA 3 cut(s) 73, 97, 106
MflI RGATCY 1 cut(s) 230
MlsI TGGCCA 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 378
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 6 cut(s) 49, 154, 175, 358, 382, 429
Mox20I TGGCCA 1 cut(s) 378
MscI TGGCCA 1 cut(s) 378
MslI CAYNNNNRTG 3 cut(s) 177, 458, 462
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 4 cut(s) 23, 227, 273, 374
MspI CCGG 6 cut(s) 303, 324, 351, 426, 432, 477
MspR9I CCNGG 5 cut(s) 304, 325, 351, 433, 477
MvnI CGCG 2 cut(s) 23, 344
MwoI GCNNNNNNNGC 4 cut(s) 32, 41, 175, 224
NciI CCSGG 5 cut(s) 304, 325, 351, 433, 477
NcoI CCATGG 1 cut(s) 186
NdeII GATC 4 cut(s) 114, 190, 230, 411
NlaIII CATG 7 cut(s) 58, 79, 130, 190, 215, 359, 461
NlaIV GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 397
OliI CACNNNNGTG 2 cut(s) 177, 458
PkrI GCNGC 8 cut(s) 22, 25, 37, 171, 205, 220, 318, 348
Ple19I CGATCG 1 cut(s) 414
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
PspN4I GGNNCC 6 cut(s) 111, 232, 361, 369, 418, 430
PspPI GGNCC 4 cut(s) 109, 251, 360, 445
PspPPI RGGWCCY 1 cut(s) 109
PsuI RGATCY 1 cut(s) 230
PvuI CGATCG 1 cut(s) 414
PvuII CAGCTG 1 cut(s) 227
RseI CAYNNNNRTG 3 cut(s) 177, 458, 462
SacII CCGCGG 1 cut(s) 24
SatI GCNGC 8 cut(s) 21, 24, 36, 170, 204, 219, 317, 347
Sau3AI GATC 4 cut(s) 114, 190, 230, 411
Sau96I GGNCC 4 cut(s) 109, 251, 360, 445
ScrFI CCNGG 5 cut(s) 304, 325, 351, 433, 477
SetI ASST 4 cut(s) 33, 37, 223, 229
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 3 cut(s) 109, 360, 445
SmiMI CAYNNNNRTG 3 cut(s) 177, 458, 462
SsiI CCGC 9 cut(s) 21, 23, 151, 204, 273, 342, 372, 402, 492
StyD4I CCNGG 5 cut(s) 302, 323, 349, 431, 475
StyI CCWWGG 2 cut(s) 66, 186
TaiI ACGT 1 cut(s) 33
TaqII GACCGA 1 cut(s) 377
TauI GCSGC 3 cut(s) 23, 26, 206
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 5 cut(s) 35, 169, 218, 316, 346
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 113
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 1 cut(s) 253
VpaK11BI GGWCC 3 cut(s) 109, 360, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.