FvH4_6g23220

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011578
Physical Location & Seq
Forward (+)
17116512 .. 17120817
4306 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g23220.t1

Sequence Viewer

Length: 1677 bp
ATGGCAGACCGGAGGGAAGGTCTTGAAGATGCACCAACATGGGCTGTATCGGTATTCGCTTTGTTTATCTTCATCTTGTCCTTCATAATCGACGATGGTATTCATCGTCTAACGAAGTTTCTTAAAGGCAGGAGAAGAAAATCCCTGAATAGGGCTTTGAAGAAGTTCAAAACAGAAATGATGCAACTAGGTTTCATATCACTGCTTCTTACTCTGTCGGAAGTACCGATATCGAATATCTGTGTCTCTCAAGTTCTGGCCATTTCTTTCCTTCCCTGTAAATATCCCCCGGACTCACCTAATGTCTCATCAGCCACACAACTTCCAGCTTCAAATACCAGCTCTTCTAAGGAAGTCGCAACAAAAGATTACTGTGAGGCAAAGGGGTTGGTTTCTCTAGTGTCAAGGGAAGGAATCCTGCAGCTAAACATCCTTATATCCGTCTTGGCAGTGTTTCATGTTCTCTACTGCATTTTAACTATGTTTCTTGGGATGGCCAAGATGAGAAAATGGAATAAATGGGAGGAAGAAACTCGGACATTAAGTTATCAGATTCTCAATGATCCAAGGAGGTTCCAGCACACCAACCAAACTCCTTTTGTGAAACGACATTTAAAGATCTGGACTAAACACCCTTTGCTGCTATGGCCTTTTGGTGGATCAATCTCAAAAGTAGATTACATGACTCTGCGAAATGGGTTCATTGCGGCCAACATCAAAGAAGGTAGCAGTTTCAATTTCCTGAACTTCCTCTCTAGAGCCTTTGATGACGATTTTGAGCAAGTGGTTGGAATCAAACAGATTGTTCTCCTCGTGGGAACAAAGCTGCAAGTTGTGATAACTAAAATGTGCGCGGAGAGTTGTAAGGAGAATCCTGTGATTCGAGGAAGCTTTGTGGTAAAACTCAACGATGATTTATTCTGGTTTGGTAAACCTAATTGGCTTCTTCATCTCCTACAATTTGTCCTAATCCAGAACTCGTTTCAGCTGGCATTCTTGACCTGGAGTTGGATTGAATATGGCCACAATTCATGCTTCAATCGAAGAACAAAAGATGTTATCATAAAGATCGCTATGGGAATTGTTGTGCAGTTGATTTGTGGTTACGTAACCCTTCCTCTTTACGCATTGGTTACCCAGATGGGGTCTGGCATGAAGAAAGCGGTATTTACTGAGCTTGTAGTTGATGGCCTAAAAAACTGGCACAAGAATGCAAGACGAAGACTATCGAAGAATGGATCCACTACTTCAAGGAAATCATCAAATTCTGCACAATTATATTCAACTGATGATGCCTCAGTTACAGACAGTGAATGTAGCTACAAAAATCCTAAACCTACCATAGTGAGAAGCTCTTCTTCTACCTCCGAAATAATTCAAGATCAAGTTCCACCTGGTTTAACTTCCTACACTGAATGTAGCTATAAAACTCCTAAGCCCACCATAACAAGAAGTTCTTCTACCTCCGAAATAATTGAAGAGCAAGCTCCAACCGATTTAACCACGAAGTCGGAAACAACAAGTTTACCAACTAGAGAAATCACCAGAGAGGAAGAGAATCCCAATATCATGAACAAGGTACTCTATGACGGTGAGATCTCATTTGGGAGCAGTTGGAAATTAAAGCTGGAGAGCGTGAGCAAGAGAATGAAGTGGGGAAATCACTTCAATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

559

Amino Acids

63.28

Weight (kDa)

9.39

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 7 - 268 1e-83 Mlo family
Mlo PF03094 268 - 428 8.2e-61 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 854
AciI CCGC 3 cut(s) 707, 854, 1163
AclWI GGATC 4 cut(s) 557, 667, 1233, 1246
AcoI YGGCCR 4 cut(s) 258, 495, 708, 1021
AcsI RAATTY 1 cut(s) 1264
AfaI GTAC 2 cut(s) 225, 1581
AfiI CCNNNNNNNGG 5 cut(s) 150, 151, 656, 1008, 1143
AjnI CCWGG 2 cut(s) 1001, 1393
Alw26I GTCTC 2 cut(s) 250, 310
AlwI GGATC 4 cut(s) 557, 667, 1233, 1246
AoxI GGCC 6 cut(s) 258, 495, 647, 708, 1021, 1189
ApeKI GCWGC 3 cut(s) 421, 640, 826
ApoI RAATTY 1 cut(s) 1264
Asp700I GAANNNNTTC 4 cut(s) 164, 1354, 1374, 1456
AspLEI GCGC 1 cut(s) 854
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 3 cut(s) 288, 1534, 1604
BalI TGGCCA 3 cut(s) 260, 497, 1023
BamHI GGATCC 1 cut(s) 1238
BarI GAAGNNNNNNTAC 2 cut(s) 1444, 1476
BauI CACGAG 1 cut(s) 812
BbsI GAAGAC 1 cut(s) 1228
BbvI GCAGC 3 cut(s) 433, 627, 813
BccI CCATC 4 cut(s) 89, 487, 1135, 1181
BciT130I CCWGG 2 cut(s) 1003, 1395
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 250, 310
BfaI CTAG 4 cut(s) 188, 398, 756, 1533
BfmI CTRYAG 1 cut(s) 419
BglII AGATCT 2 cut(s) 618, 1596
BisI GCNGC 4 cut(s) 422, 641, 708, 827
BlsI GCNGC 4 cut(s) 423, 642, 709, 828
Bme1390I CCNGG 3 cut(s) 290, 1003, 1395
BmiI GGNNCC 2 cut(s) 575, 1240
BmrFI CCNGG 3 cut(s) 290, 1003, 1395
BmsI GCATC 3 cut(s) 19, 171, 1282
BpiI GAAGAC 1 cut(s) 1228
BpmI CTGGAG 2 cut(s) 1024, 1649
Bpu10I CCTNAGC 1 cut(s) 1434
BpuEI CTTGAG 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 290
BsaAI YACGTR 1 cut(s) 1108
BsaJI CCNNGG 2 cut(s) 288, 566
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 5 cut(s) 150, 151, 656, 1008, 1143
Bse1I ACTGG 1 cut(s) 1205
Bse3DI GCAATG 1 cut(s) 702
BseBI CCWGG 2 cut(s) 1003, 1395
BseDI CCNNGG 2 cut(s) 288, 566
BseGI GGATG 2 cut(s) 429, 498
BseLI CCNNNNNNNGG 5 cut(s) 150, 151, 656, 1008, 1143
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 2 cut(s) 1164, 1311
BseNI ACTGG 1 cut(s) 1205
BseRI GAGGAG 1 cut(s) 800
BseXI GCAGC 3 cut(s) 433, 627, 813
BsgI GTGCAG 2 cut(s) 1109, 1254
Bsh1236I CGCG 1 cut(s) 854
BshFI GGCC 6 cut(s) 260, 497, 649, 710, 1023, 1191
BsiSI CCGG 2 cut(s) 10, 290
BslI CCNNNNNNNGG 5 cut(s) 150, 151, 656, 1008, 1143
BsmAI GTCTC 2 cut(s) 250, 310
BsmI GAATGC 2 cut(s) 992, 1216
BsnI GGCC 6 cut(s) 260, 497, 649, 710, 1023, 1191
Bsp143I GATC 7 cut(s) 562, 618, 659, 1068, 1238, 1381, 1596
BspACI CCGC 3 cut(s) 707, 854, 1163
BspANI GGCC 6 cut(s) 260, 497, 649, 710, 1023, 1191
BspCNI CTCAG 2 cut(s) 1165, 1310
BspFNI CGCG 1 cut(s) 854
BspHI TCATGA 1 cut(s) 1569
BspLI GGNNCC 2 cut(s) 575, 1240
BspMAI CTGCAG 1 cut(s) 423
BspPI GGATC 4 cut(s) 557, 667, 1233, 1246
BspQI GCTCTTC 3 cut(s) 349, 1360, 1473
BsrDI GCAATG 1 cut(s) 702
BsrI ACTGG 1 cut(s) 1205
BssECI CCNNGG 2 cut(s) 288, 566
BssMI GATC 7 cut(s) 562, 618, 659, 1068, 1238, 1381, 1596
BssSI CACGAG 1 cut(s) 812
BssT1I CCWWGG 1 cut(s) 566
Bst2BI CACGAG 1 cut(s) 812
Bst2UI CCWGG 2 cut(s) 1003, 1395
Bst4CI ACNGT 3 cut(s) 374, 1310, 1592
Bst6I CTCTTC 4 cut(s) 349, 1360, 1473, 1548
BstBAI YACGTR 1 cut(s) 1108
BstC8I GCNNGC 2 cut(s) 990, 1485
BstDEI CTNAG 4 cut(s) 348, 1173, 1297, 1434
BstEII GGTNACC 1 cut(s) 1132
BstF5I GGATG 2 cut(s) 429, 498
BstFNI CGCG 1 cut(s) 854
BstHHI GCGC 1 cut(s) 854
BstKTI GATC 7 cut(s) 565, 621, 662, 1071, 1241, 1384, 1599
BstMAI GTCTC 2 cut(s) 250, 310
BstMBI GATC 7 cut(s) 562, 618, 659, 1068, 1238, 1381, 1596
BstMWI GCNNNNNNNGC 1 cut(s) 646
BstNI CCWGG 2 cut(s) 1003, 1395
BstPI GGTNACC 1 cut(s) 1132
BstSCI CCNGG 3 cut(s) 288, 1001, 1393
BstSFI CTRYAG 1 cut(s) 419
BstSNI TACGTA 1 cut(s) 1108
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 3 cut(s) 433, 627, 813
BstV2I GAAGAC 1 cut(s) 1228
BstX2I RGATCY 3 cut(s) 618, 1238, 1596
BstYI RGATCY 3 cut(s) 618, 1238, 1596
BsuRI GGCC 6 cut(s) 260, 497, 649, 710, 1023, 1191
BtsCI GGATG 2 cut(s) 429, 498
BtsI GCAGTG 2 cut(s) 200, 456
BtsIMutI CAGTG 4 cut(s) 200, 456, 1315, 1410
Cac8I GCNNGC 2 cut(s) 990, 1485
CciI TCATGA 1 cut(s) 1569
CfoI GCGC 1 cut(s) 854
CsiI ACCWGGT 1 cut(s) 1393
Csp6I GTAC 2 cut(s) 224, 1580
CviAII CATG 6 cut(s) 39, 458, 682, 1032, 1153, 1570
CviQI GTAC 2 cut(s) 224, 1580
DdeI CTNAG 4 cut(s) 348, 1173, 1297, 1434
DpnI GATC 7 cut(s) 564, 620, 661, 1070, 1240, 1383, 1598
DpnII GATC 7 cut(s) 562, 618, 659, 1068, 1238, 1381, 1596
DraI TTTAAA 1 cut(s) 615
EaeI YGGCCR 4 cut(s) 258, 495, 708, 1021
Eam1104I CTCTTC 4 cut(s) 349, 1360, 1473, 1548
EarI CTCTTC 4 cut(s) 349, 1360, 1473, 1548
Eco105I TACGTA 1 cut(s) 1108
Eco130I CCWWGG 1 cut(s) 566
Eco32I GATATC 1 cut(s) 231
Eco91I GGTNACC 1 cut(s) 1132
EcoO65I GGTNACC 1 cut(s) 1132
EcoRII CCWGG 2 cut(s) 1001, 1393
EcoRV GATATC 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 566
ErhI CCWWGG 1 cut(s) 566
FaeI CATG 6 cut(s) 42, 461, 685, 1035, 1156, 1573
FalI AAGNNNNNCTT 4 cut(s) 1342, 1374, 1441, 1473
FatI CATG 6 cut(s) 38, 457, 681, 1031, 1152, 1569
Fnu4HI GCNGC 4 cut(s) 422, 641, 708, 827
FokI GGATG 2 cut(s) 416, 505
Fsp4HI GCNGC 4 cut(s) 422, 641, 708, 827
FspBI CTAG 4 cut(s) 188, 398, 756, 1533
GlaI GCGC 1 cut(s) 853
GluI GCNGC 4 cut(s) 422, 641, 708, 827
GsuI CTGGAG 2 cut(s) 1024, 1649
HaeIII GGCC 6 cut(s) 260, 497, 649, 710, 1023, 1191
HapII CCGG 2 cut(s) 10, 290
HhaI GCGC 1 cut(s) 854
Hin1II CATG 6 cut(s) 42, 461, 685, 1035, 1156, 1573
Hin6I GCGC 1 cut(s) 852
HinP1I GCGC 1 cut(s) 852
HindIII AAGCTT 1 cut(s) 889
HinfI GANTC 8 cut(s) 293, 414, 553, 685, 792, 871, 880, 1558
HpaII CCGG 2 cut(s) 10, 290
HphI GGTGA 3 cut(s) 288, 1534, 1604
Hpy166II GTNNAC 2 cut(s) 932, 1526
Hpy188I TCNGA 6 cut(s) 220, 537, 552, 1369, 1468, 1513
Hpy188III TCNNGA 8 cut(s) 23, 622, 742, 756, 973, 997, 1379, 1570
Hpy8I GTNNAC 2 cut(s) 932, 1526
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 6 cut(s) 11, 91, 281, 404, 716, 1124
HpyCH4III ACNGT 3 cut(s) 374, 1310, 1592
HpyCH4IV ACGT 1 cut(s) 1107
HpyCH4V TGCA 8 cut(s) 32, 184, 421, 471, 829, 1090, 1214, 1271
HpyF10VI GCNNNNNNNGC 1 cut(s) 646
HpyF3I CTNAG 4 cut(s) 348, 1173, 1297, 1434
HpySE526I ACGT 1 cut(s) 1107
Hsp92II CATG 6 cut(s) 42, 461, 685, 1035, 1156, 1573
HspAI GCGC 1 cut(s) 852
Kzo9I GATC 7 cut(s) 562, 618, 659, 1068, 1238, 1381, 1596
LguI GCTCTTC 3 cut(s) 349, 1360, 1473
LmnI GCTCC 2 cut(s) 1492, 1608
Lsp1109I GCAGC 3 cut(s) 433, 627, 813
LweI GCATC 3 cut(s) 19, 171, 1282
MabI ACCWGGT 1 cut(s) 1393
MaeI CTAG 4 cut(s) 188, 398, 756, 1533
MaeII ACGT 1 cut(s) 1107
MaeIII GTNAC 4 cut(s) 1103, 1108, 1132, 1300
MalI GATC 7 cut(s) 564, 620, 661, 1070, 1240, 1383, 1598
MboI GATC 7 cut(s) 562, 618, 659, 1068, 1238, 1381, 1596
MflI RGATCY 3 cut(s) 618, 1238, 1596
MlsI TGGCCA 3 cut(s) 260, 497, 1023
MluNI TGGCCA 3 cut(s) 260, 497, 1023
MlyI GAGTC 2 cut(s) 287, 679
MmeI TCCRAC 6 cut(s) 198, 769, 989, 1491, 1514, 1595
Mox20I TGGCCA 3 cut(s) 260, 497, 1023
MroXI GAANNNNTTC 4 cut(s) 164, 1354, 1374, 1456
MscI TGGCCA 3 cut(s) 260, 497, 1023
MseI TTAA 7 cut(s) 123, 476, 542, 614, 1400, 1499, 1622
MslI CAYNNNNRTG 2 cut(s) 37, 1209
Msp20I TGGCCA 3 cut(s) 260, 497, 1023
MspA1I CMGCKG 1 cut(s) 988
MspI CCGG 2 cut(s) 10, 290
MspR9I CCNGG 3 cut(s) 290, 1003, 1395
Mva1269I GAATGC 2 cut(s) 992, 1216
MvaI CCWGG 2 cut(s) 1003, 1395
MvnI CGCG 1 cut(s) 854
MwoI GCNNNNNNNGC 1 cut(s) 646
NciI CCSGG 1 cut(s) 290
NdeII GATC 7 cut(s) 562, 618, 659, 1068, 1238, 1381, 1596
NlaIII CATG 6 cut(s) 42, 461, 685, 1035, 1156, 1573
NlaIV GGNNCC 2 cut(s) 575, 1240
PagI TCATGA 1 cut(s) 1569
PciSI GCTCTTC 3 cut(s) 349, 1360, 1473
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PctI GAATGC 2 cut(s) 992, 1216
PdmI GAANNNNTTC 4 cut(s) 164, 1354, 1374, 1456
PfeI GAWTC 6 cut(s) 414, 553, 792, 871, 880, 1558
PkrI GCNGC 4 cut(s) 423, 642, 709, 828
PleI GAGTC 2 cut(s) 287, 679
PpsI GAGTC 2 cut(s) 287, 679
Ppu21I YACGTR 1 cut(s) 1108
Psp6I CCWGG 2 cut(s) 1001, 1393
PspEI GGTNACC 1 cut(s) 1132
PspGI CCWGG 2 cut(s) 1001, 1393
PspN4I GGNNCC 2 cut(s) 575, 1240
PstI CTGCAG 1 cut(s) 423
PsuI RGATCY 3 cut(s) 618, 1238, 1596
PvuII CAGCTG 1 cut(s) 988
RsaI GTAC 2 cut(s) 225, 1581
RsaNI GTAC 2 cut(s) 224, 1580
RseI CAYNNNNRTG 2 cut(s) 37, 1209
SapI GCTCTTC 3 cut(s) 349, 1360, 1473
SaqAI TTAA 7 cut(s) 123, 476, 542, 614, 1400, 1499, 1622
SatI GCNGC 4 cut(s) 422, 641, 708, 827
Sau3AI GATC 7 cut(s) 562, 618, 659, 1068, 1238, 1381, 1596
SchI GAGTC 2 cut(s) 287, 679
ScrFI CCNGG 3 cut(s) 290, 1003, 1395
SexAI ACCWGGT 1 cut(s) 1393
SfaNI GCATC 3 cut(s) 19, 171, 1282
SfcI CTRYAG 1 cut(s) 419
SmiMI CAYNNNNRTG 2 cut(s) 37, 1209
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
SnaBI TACGTA 1 cut(s) 1108
SsiI CCGC 3 cut(s) 707, 854, 1163
SspMI CTAG 4 cut(s) 188, 398, 756, 1533
StyD4I CCNGG 3 cut(s) 288, 1001, 1393
StyI CCWWGG 1 cut(s) 566
TaaI ACNGT 3 cut(s) 374, 1310, 1592
TaiI ACGT 1 cut(s) 1110
TaqI TCGA 5 cut(s) 90, 233, 883, 1042, 1229
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 6 cut(s) 414, 553, 792, 871, 880, 1558
Tru1I TTAA 7 cut(s) 123, 476, 542, 614, 1400, 1499, 1622
Tru9I TTAA 7 cut(s) 123, 476, 542, 614, 1400, 1499, 1622
TscAI CASTG 4 cut(s) 207, 456, 1315, 1417
TseI GCWGC 3 cut(s) 421, 640, 826
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 4 cut(s) 207, 456, 1315, 1417
XapI RAATTY 1 cut(s) 1264
XbaI TCTAGA 1 cut(s) 755
XcmI CCANNNNNNNNNTGG 1 cut(s) 1145
XmnI GAANNNNTTC 4 cut(s) 164, 1354, 1374, 1456
XspI CTAG 4 cut(s) 188, 398, 756, 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.