Rh3CG271200

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0011578
Physical Location & Seq
Forward (+)
26773471 .. 26782186
8716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG271200.1

Sequence Viewer

Length: 1701 bp
ATGATGCAACTGGGTTTCGTATCACTGCTTCTTACTCTGTCCGAAGTACCAATATCGAATATCTGTGTCTCTCAAGTTTTGGCAAACTCTTTTCTTCCCTGCGAATATCCCTCGGACTCAACTAGTGTCTCATCAGCCGCACAAGTTCCAGCTTCAAATACCAACTTCTCTAAAGTCACCACTCAAAATTACTGCGAGGCAAAGGGGTTGGTTTCTCTAGTGTCAACAGATGGAATCCAGCAGCTAAACGTCTTTATCTCTGTGTTAGCAGTGTTTCATGTTCTCTACTGCATCTTGACTATGTTCCTTGGGATGGCCAAGATGAAGAAATGGAATGTATGGGAGGATGAGACTCGGACACTAAACTATCAGATTCTTAATGATCCAAGGAGGTTTCAACTCACTGATCAAACTCCTTTTGGGAAACGACATCTAAAGTTCTGGAGTAAACACCCTCTTCTGCTATGGCCTGCATGTTTCGCTCGGCAGTTCAGTGGCTCAATCTCAAAAGTCGATTACATGACTCTGCGAAATGGGTTCATTGTGGCCAACTGCACAGAAGGTAGCAACTTCAACTTCCTGAACTTCCTTGCCAGAGCTTTCGACGACGATTTTGAGCAAGTGGTTGGAATCAAGTTTTGGATCTGGATCTCTTCAACTATTTTCATGTTTTTCAATGCCCACGTGTTCTATAACTATTACTGGCTACCATTCATTCCCCTACTGATTGTTCTAGTCGTGGGAACAAAGCTGCAAGTCGTGATAACTAACATGTGCGTCGAGAGTTATGAGAAAGATCCTGTGATTCGAGGAAGCTTTGTTGTGAAGCTCAAAGATGGCTTATTTTGGTTCAGTCAACCCAATTGGCTTCTTTATCTTCTCCAATTTGTCCTAATCCAGAACTCGTTTCAGCTGGCATTCTTGACCTGGACATGGTTTGAATTTGGCCAGAATTCATGCTTTAATCGAAGAACAAAAGATATCGTCATTAAGAGTAGTATGGGACTGGGTGTGCAGTTGATATGTGGTTATGTGACCCTCCCTCTTTACACACTGGTTACCCAGATGGGGTCTGGCATGAGGAAAGCAGTATTTACTGAGCTCGTAGTTGATGGCCTCAAAAACTGGCACAAGAATGCAAGACGCAGACTATCGAAGAATGGATCCTCTACTTCAAGGAAATCATCAAATTCTGCACATATTTATTCAACCGATGACTCCTCATTTACAGACAATGAATGTAGCTACAAAACTCCTAATCCCACCATAGTGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCGCGTTCCAGCCGGTTTAAGTCCGAAGAATGGATCCACTACTTCAAGGAAATCCTCCGATTCTGCACATTTTTATTCAAATGATAACTCCTCATTTCCAGACAATGAATGTAGCAACAAAAATCCTAATCCCACCATAGTGAGAAGCTCTTCTTCCACCTCCGAAATAATTGAAGAGCAAGTTCCACCCGGTTTAATTCCGAACCCGGATACTACAAGTTTACCAACTCCAGAAATCACCAGAAAGGAACAGAATCCCAACATCATCAACAAAATAATCTATGACGGTGAGATTTCTTTTGGGAGCAGTTGGAAATTAAAGCTGGAGAGCATAAGCAAGAGAAGTGGGGAAATCACTTCAATAATTGAAGACGAACTTAACTTCGATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

566

Amino Acids

64.06

Weight (kDa)

8.18

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 1 - 403 1.9e-149 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1311
AciI CCGC 1 cut(s) 138
AclWI GGATC 8 cut(s) 377, 650, 656, 791, 1158, 1171, 1335, 1348
AcoI YGGCCR 3 cut(s) 315, 546, 946
AcsI RAATTY 3 cut(s) 941, 952, 1189
AcvI CACGTG 1 cut(s) 685
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 4 cut(s) 313, 933, 1068, 1337
AflIII ACRYGT 2 cut(s) 684, 771
AhlI ACTAGT 1 cut(s) 122
AjnI CCWGG 1 cut(s) 926
AjuI GAANNNNNNNTTGG 2 cut(s) 622, 654
AleI CACNNNNGTG 2 cut(s) 1268, 1445
Alw21I GWGCWC 1 cut(s) 1104
Alw26I GTCTC 3 cut(s) 73, 133, 344
AlwI GGATC 8 cut(s) 377, 650, 656, 791, 1158, 1171, 1335, 1348
AoxI GGCC 5 cut(s) 315, 467, 546, 946, 1114
ApeKI GCWGC 2 cut(s) 241, 751
ApoI RAATTY 3 cut(s) 941, 952, 1189
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
Asp700I GAANNNNTTC 2 cut(s) 1279, 1456
AspLEI GCGC 1 cut(s) 1311
AsuC2I CCSGG 2 cut(s) 1497, 1514
AsuHPI GGTGA 3 cut(s) 169, 1537, 1607
BalI TGGCCA 3 cut(s) 317, 548, 948
BamHI GGATCC 2 cut(s) 1163, 1340
BanII GRGCYC 1 cut(s) 1104
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbrPI CACGTG 1 cut(s) 685
BbsI GAAGAC 1 cut(s) 1683
Bbv12I GWGCWC 1 cut(s) 1104
BbvI GCAGC 2 cut(s) 253, 738
BccI CCATC 5 cut(s) 224, 307, 830, 1060, 1106
BciT130I CCWGG 1 cut(s) 928
BciVI GTATCC 1 cut(s) 1510
BclI TGATCA 1 cut(s) 406
BcnI CCSGG 2 cut(s) 1497, 1514
BcoDI GTCTC 3 cut(s) 73, 133, 344
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 3 cut(s) 123, 218, 734
BfuI GTATCC 1 cut(s) 1510
BisI GCNGC 3 cut(s) 138, 242, 752
BlsI GCNGC 3 cut(s) 139, 243, 753
Bme1390I CCNGG 3 cut(s) 928, 1497, 1514
BmiI GGNNCC 2 cut(s) 1165, 1342
BmrFI CCNGG 3 cut(s) 928, 1497, 1514
BmrI ACTGGG 2 cut(s) 20, 1016
BmsI GCATC 1 cut(s) 300
BmuI ACTGGG 2 cut(s) 20, 1016
BpiI GAAGAC 1 cut(s) 1683
BpmI CTGGAG 3 cut(s) 463, 1521, 1652
BpuEI CTTGAG 1 cut(s) 57
BpuMI CCSGG 2 cut(s) 1497, 1514
BsaAI YACGTR 1 cut(s) 685
BsaBI GATNNNNATC 1 cut(s) 647
BsaJI CCNNGG 3 cut(s) 111, 307, 386
Bsc4I CCNNNNNNNGG 4 cut(s) 313, 933, 1068, 1337
Bse118I RCCGGY 1 cut(s) 1319
Bse1I ACTGG 5 cut(s) 15, 707, 1011, 1059, 1130
Bse8I GATNNNNATC 1 cut(s) 647
BseBI CCWGG 1 cut(s) 928
BseDI CCNNGG 3 cut(s) 111, 307, 386
BseGI GGATG 2 cut(s) 318, 352
BseJI GATNNNNATC 1 cut(s) 647
BseLI CCNNNNNNNGG 4 cut(s) 313, 933, 1068, 1337
BseMII CTCAG 1 cut(s) 1089
BseNI ACTGG 5 cut(s) 15, 707, 1011, 1059, 1130
BseRI GAGGAG 2 cut(s) 1210, 1387
BseXI GCAGC 2 cut(s) 253, 738
BsgI GTGCAG 4 cut(s) 538, 1034, 1179, 1356
Bsh1236I CGCG 1 cut(s) 1311
Bsh1285I CGRYCG 1 cut(s) 1696
BshFI GGCC 5 cut(s) 317, 469, 548, 948, 1116
BsiEI CGRYCG 1 cut(s) 1696
BsiHKAI GWGCWC 1 cut(s) 1104
BsiSI CCGG 3 cut(s) 1320, 1497, 1514
BslFI GGGAC 1 cut(s) 1017
BslI CCNNNNNNNGG 4 cut(s) 313, 933, 1068, 1337
BsmAI GTCTC 3 cut(s) 73, 133, 344
BsmFI GGGAC 1 cut(s) 1017
BsmI GAATGC 2 cut(s) 917, 1141
BsnI GGCC 5 cut(s) 317, 469, 548, 948, 1116
Bsp1286I GDGCHC 1 cut(s) 1104
Bsp143I GATC 8 cut(s) 382, 406, 642, 648, 796, 1163, 1340, 1693
BspACI CCGC 1 cut(s) 138
BspANI GGCC 5 cut(s) 317, 469, 548, 948, 1116
BspCNI CTCAG 1 cut(s) 1090
BspFNI CGCG 1 cut(s) 1311
BspLI GGNNCC 2 cut(s) 1165, 1342
BspPI GGATC 8 cut(s) 377, 650, 656, 791, 1158, 1171, 1335, 1348
BspQI GCTCTTC 4 cut(s) 1285, 1299, 1462, 1476
BsrFI RCCGGY 1 cut(s) 1319
BsrI ACTGG 5 cut(s) 15, 707, 1011, 1059, 1130
BssAI RCCGGY 1 cut(s) 1319
BssECI CCNNGG 3 cut(s) 111, 307, 386
BssMI GATC 8 cut(s) 382, 406, 642, 648, 796, 1163, 1340, 1693
BssT1I CCWWGG 2 cut(s) 307, 386
Bst2UI CCWGG 1 cut(s) 928
Bst4CI ACNGT 1 cut(s) 1595
Bst6I CTCTTC 6 cut(s) 462, 658, 1285, 1299, 1462, 1476
BstBAI YACGTR 1 cut(s) 685
BstC8I GCNNGC 2 cut(s) 471, 915
BstDEI CTNAG 1 cut(s) 1098
BstEII GGTNACC 1 cut(s) 1057
BstF5I GGATG 2 cut(s) 318, 352
BstFNI CGCG 1 cut(s) 1311
BstHHI GCGC 1 cut(s) 1311
BstKTI GATC 8 cut(s) 385, 409, 645, 651, 799, 1166, 1343, 1696
BstMAI GTCTC 3 cut(s) 73, 133, 344
BstMBI GATC 8 cut(s) 382, 406, 642, 648, 796, 1163, 1340, 1693
BstMCI CGRYCG 1 cut(s) 1696
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 928
BstNSI RCATGY 2 cut(s) 477, 775
BstPI GGTNACC 1 cut(s) 1057
BstSCI CCNGG 3 cut(s) 926, 1495, 1512
BstUI CGCG 1 cut(s) 1311
BstV1I GCAGC 2 cut(s) 253, 738
BstV2I GAAGAC 1 cut(s) 1683
BstX2I RGATCY 5 cut(s) 642, 648, 796, 1163, 1340
BstYI RGATCY 5 cut(s) 642, 648, 796, 1163, 1340
BsuI GTATCC 1 cut(s) 1510
BsuRI GGCC 5 cut(s) 317, 469, 548, 948, 1116
BtsCI GGATG 2 cut(s) 318, 352
BtsI GCAGTG 2 cut(s) 23, 276
BtsIMutI CAGTG 5 cut(s) 23, 276, 402, 499, 1052
Cac8I GCNNGC 2 cut(s) 471, 915
CfoI GCGC 1 cut(s) 1311
Cfr10I RCCGGY 1 cut(s) 1319
CseI GACGC 2 cut(s) 766, 1152
Csp6I GTAC 1 cut(s) 47
CspCI CAANNNNNGTGG 2 cut(s) 1633, 1668
CviAII CATG 8 cut(s) 278, 474, 520, 667, 772, 933, 957, 1078
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 1 cut(s) 1098
DpnI GATC 8 cut(s) 384, 408, 644, 650, 798, 1165, 1342, 1695
DpnII GATC 8 cut(s) 382, 406, 642, 648, 796, 1163, 1340, 1693
EaeI YGGCCR 3 cut(s) 315, 546, 946
Eam1104I CTCTTC 6 cut(s) 462, 658, 1285, 1299, 1462, 1476
EarI CTCTTC 6 cut(s) 462, 658, 1285, 1299, 1462, 1476
Ecl136II GAGCTC 1 cut(s) 1102
Eco130I CCWWGG 2 cut(s) 307, 386
Eco24I GRGCYC 1 cut(s) 1104
Eco32I GATATC 1 cut(s) 982
Eco53kI GAGCTC 1 cut(s) 1102
Eco72I CACGTG 1 cut(s) 685
Eco91I GGTNACC 1 cut(s) 1057
EcoICRI GAGCTC 1 cut(s) 1102
EcoO65I GGTNACC 1 cut(s) 1057
EcoRI GAATTC 1 cut(s) 952
EcoRII CCWGG 1 cut(s) 926
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 2 cut(s) 307, 386
EcoT38I GRGCYC 1 cut(s) 1104
ErhI CCWWGG 2 cut(s) 307, 386
FaeI CATG 8 cut(s) 281, 477, 523, 670, 775, 936, 960, 1081
FalI AAGNNNNNCTT 6 cut(s) 1267, 1299, 1444, 1476, 1668, 1700
FaqI GGGAC 1 cut(s) 1017
FatI CATG 8 cut(s) 277, 473, 519, 666, 771, 932, 956, 1077
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 3 cut(s) 138, 242, 752
FokI GGATG 2 cut(s) 325, 359
FriOI GRGCYC 1 cut(s) 1104
Fsp4HI GCNGC 3 cut(s) 138, 242, 752
FspBI CTAG 3 cut(s) 123, 218, 734
GlaI GCGC 1 cut(s) 1310
GluI GCNGC 3 cut(s) 138, 242, 752
GsuI CTGGAG 3 cut(s) 463, 1521, 1652
HaeIII GGCC 5 cut(s) 317, 469, 548, 948, 1116
HapII CCGG 3 cut(s) 1320, 1497, 1514
HgaI GACGC 2 cut(s) 766, 1152
HhaI GCGC 1 cut(s) 1311
Hin1II CATG 8 cut(s) 281, 477, 523, 670, 775, 936, 960, 1081
Hin6I GCGC 1 cut(s) 1309
HinP1I GCGC 1 cut(s) 1309
HincII GTYRAC 2 cut(s) 225, 857
HindII GTYRAC 2 cut(s) 225, 857
HindIII AAGCTT 1 cut(s) 814
HpaII CCGG 3 cut(s) 1320, 1497, 1514
HphI GGTGA 3 cut(s) 169, 1537, 1607
Hpy166II GTNNAC 4 cut(s) 225, 449, 857, 1529
Hpy188I TCNGA 9 cut(s) 43, 115, 357, 372, 1294, 1332, 1366, 1471, 1509
Hpy8I GTNNAC 4 cut(s) 225, 449, 857, 1529
Hpy99I CGWCG 3 cut(s) 608, 611, 782
HpyAV CCTTC 1 cut(s) 554
HpyCH4III ACNGT 1 cut(s) 1595
HpyCH4IV ACGT 2 cut(s) 249, 684
HpyCH4V TGCA 9 cut(s) 7, 291, 473, 555, 754, 1015, 1139, 1196, 1373
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 1 cut(s) 1098
HpySE526I ACGT 2 cut(s) 249, 684
Hsp92II CATG 8 cut(s) 281, 477, 523, 670, 775, 936, 960, 1081
HspAI GCGC 1 cut(s) 1309
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 8 cut(s) 382, 406, 642, 648, 796, 1163, 1340, 1693
LguI GCTCTTC 4 cut(s) 1285, 1299, 1462, 1476
LmnI GCTCC 1 cut(s) 1611
Lsp1109I GCAGC 2 cut(s) 253, 738
LweI GCATC 1 cut(s) 300
MaeI CTAG 3 cut(s) 123, 218, 734
MaeII ACGT 2 cut(s) 249, 684
MaeIII GTNAC 3 cut(s) 175, 1033, 1057
MalI GATC 8 cut(s) 384, 408, 644, 650, 798, 1165, 1342, 1695
MboI GATC 8 cut(s) 382, 406, 642, 648, 796, 1163, 1340, 1693
MfeI CAATTG 1 cut(s) 862
MflI RGATCY 5 cut(s) 642, 648, 796, 1163, 1340
MhlI GDGCHC 1 cut(s) 1104
MlsI TGGCCA 3 cut(s) 317, 548, 948
MluNI TGGCCA 3 cut(s) 317, 548, 948
MlyI GAGTC 4 cut(s) 110, 346, 517, 1211
MmeI TCCRAC 2 cut(s) 607, 1598
Mox20I TGGCCA 3 cut(s) 317, 548, 948
MroXI GAANNNNTTC 2 cut(s) 1279, 1456
MscI TGGCCA 3 cut(s) 317, 548, 948
MseI TTAA 7 cut(s) 378, 963, 990, 1325, 1502, 1625, 1686
MslI CAYNNNNRTG 3 cut(s) 1134, 1268, 1445
Msp20I TGGCCA 3 cut(s) 317, 548, 948
MspA1I CMGCKG 1 cut(s) 913
MspI CCGG 3 cut(s) 1320, 1497, 1514
MspR9I CCNGG 3 cut(s) 928, 1497, 1514
MunI CAATTG 1 cut(s) 862
Mva1269I GAATGC 2 cut(s) 917, 1141
MvaI CCWGG 1 cut(s) 928
MvnI CGCG 1 cut(s) 1311
MwoI GCNNNNNNNGC 1 cut(s) 479
NciI CCSGG 2 cut(s) 1497, 1514
NdeII GATC 8 cut(s) 382, 406, 642, 648, 796, 1163, 1340, 1693
NlaIII CATG 8 cut(s) 281, 477, 523, 670, 775, 936, 960, 1081
NlaIV GGNNCC 2 cut(s) 1165, 1342
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 2 cut(s) 175, 1033
NspI RCATGY 2 cut(s) 477, 775
OliI CACNNNNGTG 2 cut(s) 1268, 1445
PciI ACATGT 1 cut(s) 771
PciSI GCTCTTC 4 cut(s) 1285, 1299, 1462, 1476
PctI GAATGC 2 cut(s) 917, 1141
PdmI GAANNNNTTC 2 cut(s) 1279, 1456
PfeI GAWTC 6 cut(s) 234, 373, 630, 805, 1367, 1561
PkrI GCNGC 3 cut(s) 139, 243, 753
Ple19I CGATCG 1 cut(s) 1696
PleI GAGTC 4 cut(s) 110, 346, 517, 1211
PmaCI CACGTG 1 cut(s) 685
PmlI CACGTG 1 cut(s) 685
PpsI GAGTC 4 cut(s) 110, 346, 517, 1211
Ppu21I YACGTR 1 cut(s) 685
PscI ACATGT 1 cut(s) 771
Psp124BI GAGCTC 1 cut(s) 1104
Psp6I CCWGG 1 cut(s) 926
PspCI CACGTG 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 1057
PspGI CCWGG 1 cut(s) 926
PspN4I GGNNCC 2 cut(s) 1165, 1342
PsuI RGATCY 5 cut(s) 642, 648, 796, 1163, 1340
PvuI CGATCG 1 cut(s) 1696
PvuII CAGCTG 1 cut(s) 913
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 3 cut(s) 1134, 1268, 1445
SacI GAGCTC 1 cut(s) 1104
SapI GCTCTTC 4 cut(s) 1285, 1299, 1462, 1476
SaqAI TTAA 7 cut(s) 378, 963, 990, 1325, 1502, 1625, 1686
SatI GCNGC 3 cut(s) 138, 242, 752
Sau3AI GATC 8 cut(s) 382, 406, 642, 648, 796, 1163, 1340, 1693
SchI GAGTC 4 cut(s) 110, 346, 517, 1211
ScrFI CCNGG 3 cut(s) 928, 1497, 1514
SduI GDGCHC 1 cut(s) 1104
SfaNI GCATC 1 cut(s) 300
SmiMI CAYNNNNRTG 3 cut(s) 1134, 1268, 1445
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
SpeI ACTAGT 1 cut(s) 122
SsiI CCGC 1 cut(s) 138
SspMI CTAG 3 cut(s) 123, 218, 734
SstI GAGCTC 1 cut(s) 1104
StyD4I CCNGG 3 cut(s) 926, 1495, 1512
StyI CCWWGG 2 cut(s) 307, 386
TaaI ACNGT 1 cut(s) 1595
TaiI ACGT 2 cut(s) 252, 687
TaqI TCGA 8 cut(s) 56, 513, 603, 780, 808, 967, 1154, 1692
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 6 cut(s) 234, 373, 630, 805, 1367, 1561
Tru1I TTAA 7 cut(s) 378, 963, 990, 1325, 1502, 1625, 1686
Tru9I TTAA 7 cut(s) 378, 963, 990, 1325, 1502, 1625, 1686
TscAI CASTG 5 cut(s) 30, 276, 409, 499, 1059
TseFI GTSAC 2 cut(s) 175, 1033
TseI GCWGC 2 cut(s) 241, 751
Tsp45I GTSAC 2 cut(s) 175, 1033
TspDTI ATGAA 8 cut(s) 266, 338, 529, 655, 703, 945, 1251, 1428
TspRI CASTG 5 cut(s) 30, 276, 409, 499, 1059
XapI RAATTY 3 cut(s) 941, 952, 1189
XceI RCATGY 2 cut(s) 477, 775
XcmI CCANNNNNNNNNTGG 1 cut(s) 1070
XmnI GAANNNNTTC 2 cut(s) 1279, 1456
XspI CTAG 3 cut(s) 123, 218, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.