Rw3G021450

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011578
Physical Location & Seq
Forward (+)
25936827 .. 25945009
8183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G021450.1

Sequence Viewer

Length: 1854 bp
ATGGTCGACCAAAGGGAAGTTAGCCTTGAAGGTACTCCAACATGGGCTGTATCAGTATTTGCGTTGTTTATCTTCTTGCTATCCTTTGCCATTGACGATTGTCTTCATCGTCTAACAAAGTTTCTTAAAGGAAGGAGAAGAAAATCCCTAAATAAGGCTTTAATGAAGTTCAGAACAGAAATGATGCAACTGGGTTTCGTATCACTGCTTCTTACTCTGTCCGAAGTACCAATATCGAATATCTGTGTCTCTCAAGTTTTGGCAAACTCTTTTCTTCCCTGCGAATATCCCTCGGACTCAACTATTGTCTCATCAGCCGCACAAGTTCCAGCTTCAAATACCAACTTCTCTGAAGTCACCACTCAAAATTACTGCGAGGCAAAGGGGTTGGTTTCTCTAGTGTCAACAGATGGAATCCAGCAGCTAAACATCTTTATCTCTGTGTTAGCAGTGTTTCATGTTCTCTACTGCATCTTGACTATGTTCCTTGGGATGGCCAAGATGAAGAAATGGAATCTATGGGAGGATGAGACTCGGACACTAAACTATCAGATTCTTAATGATCCAAGGAGGTTTCAACTCACTGATCAAACTCCTTTTGGGAAACGACATCTAAAGTTTGGAGCATGTTTCGCTCGGCAGTTCAGTGGCTCAATCTCAAAAGTCGATTACATGACTCTGCGAAATGGGTTCATTGTGGCCAACTGCACAGAAGGTAGCAACTTCAACTTCCTGAACTTCCTTGCCAGAGCTTTCGACGACGATTATGAGCAAGTGGTTGTAATCAAGTTTTGGATCTGGATCTCTTCAACTATTTTCATGTTTTTCAATGCCCACGTGTTCTATAACTATTACTGGCTACCATTCATTCCCCTACTGATTGTTCTAGTCGTGGGAACAAAGTTGCAAGTCGTGATAACTAACATGTGCGTCGAGAGTTATGAGAAAGATCCTGTGATTCGAGGAAGCTTTGTTGTGAAGCTCAAAGATGGCTTATTTTGGTTCAGTCAACCCAATTGGCTTCTTCATCTTCTCCAATTTGTCCTAATCCAGAACTCGTTTCAGCTGGCATTCTTGACCTGGACATGGTTTGAATTTGGCCAGAATTCATGCTTTAATCGAAGAACAAAAGATATCGTCATTAAGAGTAGTATGGGACTGGGTGTGCAGTTGATATGTGGTTATGTGACCCTCCCTCTTTACGCACTGGTTACCCAGATGGGGTCTGGCATGAGGAAAGCAGTATTTACTGAGCTCGTAGTTGATGGCCTCAAAAACTGGCACAAGAATGCAAGACGCAGACTATCGAAGAATGGATCCTCTACTTCAAGGAAATCATCAAATTCTGCACATATTTATTCAACCGATGACTCCTCATTTACAGACAATGAATGTAGCTACAAAACTCCTAATCCCACCATAGTGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCGCGTTCCAGCTGGTTTAAGTCCGAAGAATGGATCCACTACTTCAAGGAAATCCTCCGATTCTGCACATTTTTATTCAAATGATAACTCCTCATTTCCAGACAATGAATGTAGCAACAAAAATCCTAATCCCACCATAGTGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCAAGTTCCACCCGGTTTAATTCCGAACCCGGATACTACAAGTTTACCAACTCCAGAAATCACCAGAGAGGAACAGAATCCCAACATCATCAACAAAATAATCTATGACGGTGAGATTTCTTTTGGGAGCAGTTGGAAATTAAAGCTGGAGAGCATGAGCAAGAGAAGTGGGGAAATCACTTCAATAATTGAAGACGAACTTAACTTCGATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

617

Amino Acids

69.95

Weight (kDa)

8.14

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 7 - 454 3.8e-158 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 6
AccII CGCG 1 cut(s) 1464
AciI CCGC 1 cut(s) 318
AclWI GGATC 8 cut(s) 557, 803, 809, 944, 1311, 1324, 1488, 1501
AcoI YGGCCR 3 cut(s) 495, 699, 1099
AcsI RAATTY 3 cut(s) 1094, 1105, 1342
AcuI CTGAAG 1 cut(s) 372
AcvI CACGTG 1 cut(s) 838
AfaI GTAC 2 cut(s) 34, 228
AfiI CCNNNNNNNGG 5 cut(s) 154, 493, 1086, 1221, 1490
AflIII ACRYGT 2 cut(s) 837, 924
AjnI CCWGG 1 cut(s) 1079
AleI CACNNNNGTG 2 cut(s) 1421, 1598
Alw21I GWGCWC 1 cut(s) 1257
Alw26I GTCTC 3 cut(s) 253, 313, 524
AlwI GGATC 8 cut(s) 557, 803, 809, 944, 1311, 1324, 1488, 1501
AoxI GGCC 4 cut(s) 495, 699, 1099, 1267
ApeKI GCWGC 1 cut(s) 421
ApoI RAATTY 3 cut(s) 1094, 1105, 1342
Asp700I GAANNNNTTC 2 cut(s) 1432, 1609
AspLEI GCGC 1 cut(s) 1464
AsuC2I CCSGG 2 cut(s) 1650, 1667
AsuHPI GGTGA 3 cut(s) 349, 1690, 1760
BalI TGGCCA 3 cut(s) 497, 701, 1101
BamHI GGATCC 2 cut(s) 1316, 1493
BanII GRGCYC 1 cut(s) 1257
BarI GAAGNNNNNNTAC 2 cut(s) 192, 224
BbrPI CACGTG 1 cut(s) 838
BbsI GAAGAC 2 cut(s) 95, 1836
Bbv12I GWGCWC 1 cut(s) 1257
BbvI GCAGC 1 cut(s) 433
BccI CCATC 5 cut(s) 404, 487, 983, 1213, 1259
BciT130I CCWGG 1 cut(s) 1081
BciVI GTATCC 1 cut(s) 1663
BclI TGATCA 1 cut(s) 586
BcnI CCSGG 2 cut(s) 1650, 1667
BcoDI GTCTC 3 cut(s) 253, 313, 524
BfaI CTAG 2 cut(s) 398, 887
BfuI GTATCC 1 cut(s) 1663
BisI GCNGC 2 cut(s) 318, 422
BlsI GCNGC 2 cut(s) 319, 423
Bme1390I CCNGG 3 cut(s) 1081, 1650, 1667
BmiI GGNNCC 2 cut(s) 1318, 1495
BmrFI CCNGG 3 cut(s) 1081, 1650, 1667
BmrI ACTGGG 2 cut(s) 200, 1169
BmsI GCATC 2 cut(s) 174, 480
BmuI ACTGGG 2 cut(s) 200, 1169
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 95, 1836
BpmI CTGGAG 2 cut(s) 1674, 1805
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 2 cut(s) 1650, 1667
BsaAI YACGTR 1 cut(s) 838
BsaBI GATNNNNATC 1 cut(s) 800
BsaJI CCNNGG 3 cut(s) 291, 487, 566
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 493, 1086, 1221, 1490
Bse1I ACTGG 5 cut(s) 195, 860, 1164, 1212, 1283
Bse8I GATNNNNATC 1 cut(s) 800
BseBI CCWGG 1 cut(s) 1081
BseDI CCNNGG 3 cut(s) 291, 487, 566
BseGI GGATG 2 cut(s) 498, 532
BseJI GATNNNNATC 1 cut(s) 800
BseLI CCNNNNNNNGG 5 cut(s) 154, 493, 1086, 1221, 1490
BseMII CTCAG 1 cut(s) 1242
BseNI ACTGG 5 cut(s) 195, 860, 1164, 1212, 1283
BseRI GAGGAG 2 cut(s) 1363, 1540
BseXI GCAGC 1 cut(s) 433
BsgI GTGCAG 4 cut(s) 691, 1187, 1332, 1509
Bsh1236I CGCG 1 cut(s) 1464
Bsh1285I CGRYCG 1 cut(s) 1849
BshFI GGCC 4 cut(s) 497, 701, 1101, 1269
BsiEI CGRYCG 1 cut(s) 1849
BsiHKAI GWGCWC 1 cut(s) 1257
BsiSI CCGG 2 cut(s) 1650, 1667
BslFI GGGAC 1 cut(s) 1170
BslI CCNNNNNNNGG 5 cut(s) 154, 493, 1086, 1221, 1490
BsmAI GTCTC 3 cut(s) 253, 313, 524
BsmFI GGGAC 1 cut(s) 1170
BsmI GAATGC 2 cut(s) 1070, 1294
BsnI GGCC 4 cut(s) 497, 701, 1101, 1269
Bsp1286I GDGCHC 1 cut(s) 1257
Bsp143I GATC 8 cut(s) 562, 586, 795, 801, 949, 1316, 1493, 1846
BspACI CCGC 1 cut(s) 318
BspANI GGCC 4 cut(s) 497, 701, 1101, 1269
BspCNI CTCAG 1 cut(s) 1243
BspFNI CGCG 1 cut(s) 1464
BspLI GGNNCC 2 cut(s) 1318, 1495
BspPI GGATC 8 cut(s) 557, 803, 809, 944, 1311, 1324, 1488, 1501
BspQI GCTCTTC 4 cut(s) 1438, 1452, 1615, 1629
BsrI ACTGG 5 cut(s) 195, 860, 1164, 1212, 1283
BssECI CCNNGG 3 cut(s) 291, 487, 566
BssMI GATC 8 cut(s) 562, 586, 795, 801, 949, 1316, 1493, 1846
BssT1I CCWWGG 2 cut(s) 487, 566
Bst2UI CCWGG 1 cut(s) 1081
Bst4CI ACNGT 1 cut(s) 1748
Bst6I CTCTTC 5 cut(s) 811, 1438, 1452, 1615, 1629
BstBAI YACGTR 1 cut(s) 838
BstC8I GCNNGC 1 cut(s) 1068
BstDEI CTNAG 1 cut(s) 1251
BstEII GGTNACC 1 cut(s) 1210
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 2 cut(s) 498, 532
BstFNI CGCG 1 cut(s) 1464
BstHHI GCGC 1 cut(s) 1464
BstKTI GATC 8 cut(s) 565, 589, 798, 804, 952, 1319, 1496, 1849
BstMAI GTCTC 3 cut(s) 253, 313, 524
BstMBI GATC 8 cut(s) 562, 586, 795, 801, 949, 1316, 1493, 1846
BstMCI CGRYCG 1 cut(s) 1849
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 1 cut(s) 1081
BstNSI RCATGY 2 cut(s) 630, 928
BstPAI GACNNNNGTC 1 cut(s) 99
BstPI GGTNACC 1 cut(s) 1210
BstSCI CCNGG 3 cut(s) 1079, 1648, 1665
BstUI CGCG 1 cut(s) 1464
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 2 cut(s) 95, 1836
BstX2I RGATCY 5 cut(s) 795, 801, 949, 1316, 1493
BstYI RGATCY 5 cut(s) 795, 801, 949, 1316, 1493
BsuI GTATCC 1 cut(s) 1663
BsuRI GGCC 4 cut(s) 497, 701, 1101, 1269
BtsCI GGATG 2 cut(s) 498, 532
BtsI GCAGTG 2 cut(s) 203, 456
BtsIMutI CAGTG 5 cut(s) 203, 456, 582, 652, 1205
Cac8I GCNNGC 1 cut(s) 1068
CfoI GCGC 1 cut(s) 1464
CseI GACGC 2 cut(s) 919, 1305
Csp6I GTAC 2 cut(s) 33, 227
CspCI CAANNNNNGTGG 2 cut(s) 1786, 1821
CviQI GTAC 2 cut(s) 33, 227
DdeI CTNAG 1 cut(s) 1251
DpnI GATC 8 cut(s) 564, 588, 797, 803, 951, 1318, 1495, 1848
DpnII GATC 8 cut(s) 562, 586, 795, 801, 949, 1316, 1493, 1846
EaeI YGGCCR 3 cut(s) 495, 699, 1099
Eam1104I CTCTTC 5 cut(s) 811, 1438, 1452, 1615, 1629
EarI CTCTTC 5 cut(s) 811, 1438, 1452, 1615, 1629
Ecl136II GAGCTC 1 cut(s) 1255
Eco130I CCWWGG 2 cut(s) 487, 566
Eco24I GRGCYC 1 cut(s) 1257
Eco32I GATATC 1 cut(s) 1135
Eco53kI GAGCTC 1 cut(s) 1255
Eco57I CTGAAG 1 cut(s) 372
Eco72I CACGTG 1 cut(s) 838
Eco91I GGTNACC 1 cut(s) 1210
EcoICRI GAGCTC 1 cut(s) 1255
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoO65I GGTNACC 1 cut(s) 1210
EcoRI GAATTC 1 cut(s) 1105
EcoRII CCWGG 1 cut(s) 1079
EcoRV GATATC 1 cut(s) 1135
EcoT14I CCWWGG 2 cut(s) 487, 566
EcoT38I GRGCYC 1 cut(s) 1257
ErhI CCWWGG 2 cut(s) 487, 566
FalI AAGNNNNNCTT 8 cut(s) 9, 41, 1420, 1452, 1597, 1629, 1821, 1853
FaqI GGGAC 1 cut(s) 1170
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 2 cut(s) 318, 422
FokI GGATG 2 cut(s) 505, 539
FriOI GRGCYC 1 cut(s) 1257
Fsp4HI GCNGC 2 cut(s) 318, 422
FspBI CTAG 2 cut(s) 398, 887
GlaI GCGC 1 cut(s) 1463
GluI GCNGC 2 cut(s) 318, 422
GsuI CTGGAG 2 cut(s) 1674, 1805
HaeIII GGCC 4 cut(s) 497, 701, 1101, 1269
HapII CCGG 2 cut(s) 1650, 1667
HgaI GACGC 2 cut(s) 919, 1305
HhaI GCGC 1 cut(s) 1464
Hin6I GCGC 1 cut(s) 1462
HinP1I GCGC 1 cut(s) 1462
HincII GTYRAC 3 cut(s) 7, 405, 1010
HindII GTYRAC 3 cut(s) 7, 405, 1010
HindIII AAGCTT 1 cut(s) 967
HpaII CCGG 2 cut(s) 1650, 1667
HphI GGTGA 3 cut(s) 349, 1690, 1760
Hpy166II GTNNAC 4 cut(s) 7, 405, 1010, 1682
Hpy188III TCNNGA 9 cut(s) 475, 733, 799, 913, 934, 1051, 1075, 1559, 1691
Hpy8I GTNNAC 4 cut(s) 7, 405, 1010, 1682
Hpy99I CGWCG 3 cut(s) 761, 764, 935
HpyAV CCTTC 3 cut(s) 23, 126, 707
HpyCH4III ACNGT 1 cut(s) 1748
HpyCH4IV ACGT 1 cut(s) 837
HpyCH4V TGCA 8 cut(s) 187, 471, 708, 907, 1168, 1292, 1349, 1526
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 1 cut(s) 1251
HpySE526I ACGT 1 cut(s) 837
HspAI GCGC 1 cut(s) 1462
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 8 cut(s) 562, 586, 795, 801, 949, 1316, 1493, 1846
LguI GCTCTTC 4 cut(s) 1438, 1452, 1615, 1629
LmnI GCTCC 2 cut(s) 623, 1764
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 2 cut(s) 174, 480
MaeI CTAG 2 cut(s) 398, 887
MaeII ACGT 1 cut(s) 837
MaeIII GTNAC 3 cut(s) 355, 1186, 1210
MalI GATC 8 cut(s) 564, 588, 797, 803, 951, 1318, 1495, 1848
MboI GATC 8 cut(s) 562, 586, 795, 801, 949, 1316, 1493, 1846
MfeI CAATTG 1 cut(s) 1015
MflI RGATCY 5 cut(s) 795, 801, 949, 1316, 1493
MhlI GDGCHC 1 cut(s) 1257
MlsI TGGCCA 3 cut(s) 497, 701, 1101
MluNI TGGCCA 3 cut(s) 497, 701, 1101
MlyI GAGTC 4 cut(s) 290, 526, 670, 1364
MmeI TCCRAC 2 cut(s) 62, 1751
Mox20I TGGCCA 3 cut(s) 497, 701, 1101
MroXI GAANNNNTTC 2 cut(s) 1432, 1609
MscI TGGCCA 3 cut(s) 497, 701, 1101
MseI TTAA 9 cut(s) 126, 161, 558, 1116, 1143, 1478, 1655, 1778, 1839
MslI CAYNNNNRTG 3 cut(s) 1287, 1421, 1598
Msp20I TGGCCA 3 cut(s) 497, 701, 1101
MspA1I CMGCKG 2 cut(s) 1066, 1472
MspI CCGG 2 cut(s) 1650, 1667
MspR9I CCNGG 3 cut(s) 1081, 1650, 1667
MunI CAATTG 1 cut(s) 1015
Mva1269I GAATGC 2 cut(s) 1070, 1294
MvaI CCWGG 1 cut(s) 1081
MvnI CGCG 1 cut(s) 1464
MwoI GCNNNNNNNGC 1 cut(s) 632
NciI CCSGG 2 cut(s) 1650, 1667
NdeII GATC 8 cut(s) 562, 586, 795, 801, 949, 1316, 1493, 1846
NlaIV GGNNCC 2 cut(s) 1318, 1495
NmeAIII GCCGAG 1 cut(s) 616
NmuCI GTSAC 2 cut(s) 355, 1186
NspI RCATGY 2 cut(s) 630, 928
OliI CACNNNNGTG 2 cut(s) 1421, 1598
PciI ACATGT 1 cut(s) 924
PciSI GCTCTTC 4 cut(s) 1438, 1452, 1615, 1629
PctI GAATGC 2 cut(s) 1070, 1294
PdmI GAANNNNTTC 2 cut(s) 1432, 1609
PfeI GAWTC 6 cut(s) 414, 514, 553, 958, 1520, 1714
PkrI GCNGC 2 cut(s) 319, 423
Ple19I CGATCG 1 cut(s) 1849
PleI GAGTC 4 cut(s) 290, 526, 670, 1364
PmaCI CACGTG 1 cut(s) 838
PmlI CACGTG 1 cut(s) 838
PpsI GAGTC 4 cut(s) 290, 526, 670, 1364
Ppu21I YACGTR 1 cut(s) 838
PscI ACATGT 1 cut(s) 924
PshAI GACNNNNGTC 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 1257
Psp6I CCWGG 1 cut(s) 1079
PspCI CACGTG 1 cut(s) 838
PspEI GGTNACC 1 cut(s) 1210
PspGI CCWGG 1 cut(s) 1079
PspN4I GGNNCC 2 cut(s) 1318, 1495
PsuI RGATCY 5 cut(s) 795, 801, 949, 1316, 1493
PvuI CGATCG 1 cut(s) 1849
PvuII CAGCTG 2 cut(s) 1066, 1472
RsaI GTAC 2 cut(s) 34, 228
RsaNI GTAC 2 cut(s) 33, 227
RseI CAYNNNNRTG 3 cut(s) 1287, 1421, 1598
SacI GAGCTC 1 cut(s) 1257
SalI GTCGAC 1 cut(s) 5
SapI GCTCTTC 4 cut(s) 1438, 1452, 1615, 1629
SaqAI TTAA 9 cut(s) 126, 161, 558, 1116, 1143, 1478, 1655, 1778, 1839
SatI GCNGC 2 cut(s) 318, 422
Sau3AI GATC 8 cut(s) 562, 586, 795, 801, 949, 1316, 1493, 1846
SchI GAGTC 4 cut(s) 290, 526, 670, 1364
ScrFI CCNGG 3 cut(s) 1081, 1650, 1667
SduI GDGCHC 1 cut(s) 1257
SfaNI GCATC 2 cut(s) 174, 480
SmiMI CAYNNNNRTG 3 cut(s) 1287, 1421, 1598
SmlI CTYRAG 1 cut(s) 252
SmoI CTYRAG 1 cut(s) 252
SsiI CCGC 1 cut(s) 318
SspMI CTAG 2 cut(s) 398, 887
SstI GAGCTC 1 cut(s) 1257
StyD4I CCNGG 3 cut(s) 1079, 1648, 1665
StyI CCWWGG 2 cut(s) 487, 566
TaaI ACNGT 1 cut(s) 1748
TaiI ACGT 1 cut(s) 840
TaqI TCGA 9 cut(s) 6, 236, 666, 756, 933, 961, 1120, 1307, 1845
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 6 cut(s) 414, 514, 553, 958, 1520, 1714
Tru1I TTAA 9 cut(s) 126, 161, 558, 1116, 1143, 1478, 1655, 1778, 1839
Tru9I TTAA 9 cut(s) 126, 161, 558, 1116, 1143, 1478, 1655, 1778, 1839
TscAI CASTG 5 cut(s) 210, 456, 589, 652, 1212
TseFI GTSAC 2 cut(s) 355, 1186
TseI GCWGC 1 cut(s) 421
Tsp45I GTSAC 2 cut(s) 355, 1186
TspRI CASTG 5 cut(s) 210, 456, 589, 652, 1212
XagI CCTNNNNNAGG 1 cut(s) 152
XapI RAATTY 3 cut(s) 1094, 1105, 1342
XceI RCATGY 2 cut(s) 630, 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 1223
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 2 cut(s) 1432, 1609
XspI CTAG 2 cut(s) 398, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.