Rh3BG275300

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011578
Physical Location & Seq
Forward (+)
26951884 .. 26967345
15462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG275300.1

Sequence Viewer

Length: 1251 bp
ATGGCCGACCAGAGGAAAGGTAGCCTTGAAGATACACCAACATGGGCTGTATCAGTATTTGCTTTGTTTATCTTCGTGCTATCCTTTATAATCGATGATGGTCTTCATCGTCTAACGAAGTTTCTTAAAGGAAGGAGAAGAAAATCCCTGAATAGGGCTTTGAAGAAGTTCAAAACAGAAATGATGCAACTGGGTTTCGTATCACTGCTTCTTACTCTGTCGGAAGTACCAATATCGAATATCTGTGTCTCTCAAGTTGTGGCAAACTCTTTTCTTCCCTGTGAATATCCCCTGGATTCAACTACTGTCTCATCAGCCGCACAACTTCCAGCTTCAAATACCAACTTCTCTAAGGAAGTCACCACTCAAACTTACTGCGAGGCAAAGGGGTTGGTTTCTCTAGTGTCAAGGGAAGGAATCCTGCAGCTAAACATCTTTATAGCCGTCTTGGCAGTGTTTCATGTTCTCTACTGCATCTTAACTATGTTTCTTGGGATGGCCAAGATGAGAAAATGGAATGAATGGGAGGATGAAACTCAGACACTAAATTATCAGATTCTTAATGATCCAAGGAGGTTCCAGCACACCCATCAAACTCCTTTTGGGAAACGACATCTAAAGATCTGGAGTAAACACCCTCTCCTGCTATGGCCTGTTTCTTTTGCTCGGCAGTTTGGTGGCTCAGTATTAAAAGTAGATTACATGACTCTGCGAAATGGTTTCATTGTGGCCAACATCAAAGAAGGTAGCAGCTTCAATTTCCTGAACTTCCTTGCTAGAGCTTTCGATGACGATTTTGAGCAAGTGGTTGGAATCAAGTTTTGGATTTGGATCTTTTCAATTCTATTCATTTTTTTCAATGCTCATGTGTTCTACAACTATTATTGGCTACCATTCATTCCCTTACTGATTGTTCTACTCGTGGGAACAAAGCTGCAAGTCGTGATAACTAAAATGTGCGCGGAGAGTTGTAAGAAGAATCCTGTGATTCGCGGAAGCTTTAAGGTGGAGCTCAATAATGATTTATTCTGGTTTGGTCAACCCCATTGGCTTCTTCATCTCCTACAGTTTGTCCTAATCCAGAACTCGTTTCAGCTGGCTTTCTTGACCTGGAGTTGGATTGAGTATGGCCAAAGTTCATGCTTTAATCGAAGAACAAAAGATGTTATCATAAAGAGTACTATGGGAATTTTTGTGCAGTTGATTTGTGGTTATGTAACCCTCCCTCTTTATGCACTGGTTAACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

416

Amino Acids

48.12

Weight (kDa)

9.35

Isoelectric Point (pI)

32.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 416 3.6e-154 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
AccII CGCG 2 cut(s) 962, 993
AciI CCGC 3 cut(s) 318, 962, 993
AclWI GGATC 2 cut(s) 560, 839
AcoI YGGCCR 4 cut(s) 3, 498, 729, 1129
AcsI RAATTY 1 cut(s) 1188
AfaI GTAC 2 cut(s) 228, 1180
AfiI CCNNNNNNNGG 4 cut(s) 12, 153, 154, 1116
AgsI TTSAA 8 cut(s) 29, 163, 172, 300, 336, 757, 840, 859
AjnI CCWGG 2 cut(s) 291, 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 805, 837
AluBI AGCT 8 cut(s) 332, 427, 753, 782, 934, 999, 1012, 1096
AluI AGCT 8 cut(s) 332, 427, 753, 782, 934, 999, 1012, 1096
Alw21I GWGCWC 1 cut(s) 1014
Alw26I GTCTC 2 cut(s) 253, 313
AlwI GGATC 2 cut(s) 560, 839
AoxI GGCC 5 cut(s) 3, 498, 650, 729, 1129
ApeKI GCWGC 3 cut(s) 424, 750, 934
ApoI RAATTY 1 cut(s) 1188
Asp700I GAANNNNTTC 1 cut(s) 167
AspLEI GCGC 1 cut(s) 962
AsuHPI GGTGA 1 cut(s) 352
BalI TGGCCA 3 cut(s) 500, 731, 1131
BanII GRGCYC 1 cut(s) 1014
BarI GAAGNNNNNNTAC 2 cut(s) 192, 224
BauI CACGAG 1 cut(s) 920
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 1014
BbvI GCAGC 3 cut(s) 436, 762, 921
BccI CCATC 3 cut(s) 92, 490, 597
BceAI ACGGC 1 cut(s) 428
BciT130I CCWGG 2 cut(s) 293, 1111
BcoDI GTCTC 2 cut(s) 253, 313
BfaI CTAG 2 cut(s) 401, 777
BfmI CTRYAG 2 cut(s) 422, 1064
BglI GCCNNNNNGGC 1 cut(s) 449
BglII AGATCT 1 cut(s) 621
BisI GCNGC 4 cut(s) 318, 425, 751, 935
BlsI GCNGC 4 cut(s) 319, 426, 752, 936
BmcAI AGTACT 1 cut(s) 1180
Bme1390I CCNGG 2 cut(s) 293, 1111
BmiI GGNNCC 1 cut(s) 578
BmrFI CCNGG 2 cut(s) 293, 1111
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 2 cut(s) 174, 483
BmuI ACTGGG 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 95
BpmI CTGGAG 2 cut(s) 646, 1132
BpuEI CTTGAG 1 cut(s) 237
Bsa29I ATCGAT 1 cut(s) 93
BsaBI GATNNNNATC 1 cut(s) 830
BsaJI CCNNGG 2 cut(s) 291, 569
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 153, 154, 1116
Bse1I ACTGG 3 cut(s) 195, 1242, 1246
Bse8I GATNNNNATC 1 cut(s) 830
BseBI CCWGG 2 cut(s) 293, 1111
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 291, 569
BseGI GGATG 2 cut(s) 501, 535
BseJI GATNNNNATC 1 cut(s) 830
BseLI CCNNNNNNNGG 4 cut(s) 12, 153, 154, 1116
BseMII CTCAG 2 cut(s) 551, 696
BseNI ACTGG 3 cut(s) 195, 1242, 1246
BseXI GCAGC 3 cut(s) 436, 762, 921
BsgI GTGCAG 1 cut(s) 1217
Bsh1236I CGCG 2 cut(s) 962, 993
BshFI GGCC 5 cut(s) 5, 500, 652, 731, 1131
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 1014
BslI CCNNNNNNNGG 4 cut(s) 12, 153, 154, 1116
BsmAI GTCTC 2 cut(s) 253, 313
BsnI GGCC 5 cut(s) 5, 500, 652, 731, 1131
Bsp1286I GDGCHC 1 cut(s) 1014
Bsp143I GATC 3 cut(s) 565, 621, 831
BspACI CCGC 3 cut(s) 318, 962, 993
BspANI GGCC 5 cut(s) 5, 500, 652, 731, 1131
BspCNI CTCAG 2 cut(s) 550, 695
BspDI ATCGAT 1 cut(s) 93
BspFNI CGCG 2 cut(s) 962, 993
BspLI GGNNCC 1 cut(s) 578
BspMAI CTGCAG 1 cut(s) 426
BspPI GGATC 2 cut(s) 560, 839
BsrI ACTGG 3 cut(s) 195, 1242, 1246
BssECI CCNNGG 2 cut(s) 291, 569
BssMI GATC 3 cut(s) 565, 621, 831
BssSI CACGAG 1 cut(s) 920
BssT1I CCWWGG 1 cut(s) 569
Bst2BI CACGAG 1 cut(s) 920
Bst2UI CCWGG 2 cut(s) 293, 1111
Bst4CI ACNGT 2 cut(s) 307, 1068
BstC8I GCNNGC 1 cut(s) 1098
BstDEI CTNAG 3 cut(s) 351, 537, 682
BstF5I GGATG 2 cut(s) 501, 535
BstFNI CGCG 2 cut(s) 962, 993
BstHHI GCGC 1 cut(s) 962
BstKTI GATC 3 cut(s) 568, 624, 834
BstMAI GTCTC 2 cut(s) 253, 313
BstMBI GATC 3 cut(s) 565, 621, 831
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 2 cut(s) 293, 1111
BstSCI CCNGG 2 cut(s) 291, 1109
BstSFI CTRYAG 2 cut(s) 422, 1064
BstUI CGCG 2 cut(s) 962, 993
BstV1I GCAGC 3 cut(s) 436, 762, 921
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 2 cut(s) 621, 831
BstYI RGATCY 2 cut(s) 621, 831
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 5 cut(s) 5, 500, 652, 731, 1131
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 2 cut(s) 501, 535
BtsI GCAGTG 2 cut(s) 203, 459
BtsIMutI CAGTG 3 cut(s) 203, 459, 1235
Cac8I GCNNGC 1 cut(s) 1098
CfoI GCGC 1 cut(s) 962
ClaI ATCGAT 1 cut(s) 93
Csp6I GTAC 2 cut(s) 227, 1179
CviAII CATG 5 cut(s) 42, 461, 703, 866, 1140
CviQI GTAC 2 cut(s) 227, 1179
DdeI CTNAG 3 cut(s) 351, 537, 682
DpnI GATC 3 cut(s) 567, 623, 833
DpnII GATC 3 cut(s) 565, 621, 831
EaeI YGGCCR 4 cut(s) 3, 498, 729, 1129
Ecl136II GAGCTC 1 cut(s) 1012
Eco130I CCWWGG 1 cut(s) 569
Eco24I GRGCYC 1 cut(s) 1014
Eco53kI GAGCTC 1 cut(s) 1012
EcoICRI GAGCTC 1 cut(s) 1012
EcoRII CCWGG 2 cut(s) 291, 1109
EcoT14I CCWWGG 1 cut(s) 569
EcoT38I GRGCYC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 569
FaeI CATG 5 cut(s) 45, 464, 706, 869, 1143
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FatI CATG 5 cut(s) 41, 460, 702, 865, 1139
Fnu4HI GCNGC 4 cut(s) 318, 425, 751, 935
FokI GGATG 2 cut(s) 508, 542
FriOI GRGCYC 1 cut(s) 1014
Fsp4HI GCNGC 4 cut(s) 318, 425, 751, 935
FspBI CTAG 2 cut(s) 401, 777
GlaI GCGC 1 cut(s) 961
GluI GCNGC 4 cut(s) 318, 425, 751, 935
GsuI CTGGAG 2 cut(s) 646, 1132
HaeIII GGCC 5 cut(s) 5, 500, 652, 731, 1131
HhaI GCGC 1 cut(s) 962
Hin1II CATG 5 cut(s) 45, 464, 706, 869, 1143
Hin6I GCGC 1 cut(s) 960
HinP1I GCGC 1 cut(s) 960
HincII GTYRAC 2 cut(s) 1040, 1243
HindII GTYRAC 2 cut(s) 1040, 1243
HindIII AAGCTT 1 cut(s) 997
HinfI GANTC 7 cut(s) 296, 417, 556, 706, 813, 979, 988
HpaI GTTAAC 1 cut(s) 1243
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 3 cut(s) 632, 1040, 1243
Hpy188I TCNGA 3 cut(s) 223, 540, 555
Hpy188III TCNNGA 5 cut(s) 625, 763, 943, 1081, 1105
Hpy8I GTNNAC 3 cut(s) 632, 1040, 1243
HpyAV CCTTC 3 cut(s) 126, 407, 737
HpyCH4III ACNGT 2 cut(s) 307, 1068
HpyCH4V TGCA 6 cut(s) 187, 424, 474, 937, 1198, 1235
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 3 cut(s) 351, 537, 682
Hsp92II CATG 5 cut(s) 45, 464, 706, 869, 1143
HspAI GCGC 1 cut(s) 960
KspAI GTTAAC 1 cut(s) 1243
Kzo9I GATC 3 cut(s) 565, 621, 831
LmnI GCTCC 1 cut(s) 1009
Lsp1109I GCAGC 3 cut(s) 436, 762, 921
LweI GCATC 2 cut(s) 174, 483
MaeI CTAG 2 cut(s) 401, 777
MaeIII GTNAC 2 cut(s) 358, 1216
MalI GATC 3 cut(s) 567, 623, 833
MboI GATC 3 cut(s) 565, 621, 831
MboII GAAGA 9 cut(s) 41, 64, 95, 150, 175, 266, 988, 1046, 1164
MflI RGATCY 2 cut(s) 621, 831
MhlI GDGCHC 1 cut(s) 1014
MlsI TGGCCA 3 cut(s) 500, 731, 1131
MluCI AATT 4 cut(s) 547, 757, 840, 1188
MluNI TGGCCA 3 cut(s) 500, 731, 1131
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 3 cut(s) 201, 790, 1097
MnlI CCTC 7 cut(s) 6, 373, 520, 567, 648, 1232, 1236
Mox20I TGGCCA 3 cut(s) 500, 731, 1131
MroXI GAANNNNTTC 1 cut(s) 167
MscI TGGCCA 3 cut(s) 500, 731, 1131
MseI TTAA 7 cut(s) 126, 479, 561, 689, 1002, 1146, 1242
MslI CAYNNNNRTG 1 cut(s) 40
Msp20I TGGCCA 3 cut(s) 500, 731, 1131
MspA1I CMGCKG 1 cut(s) 1096
MspR9I CCNGG 2 cut(s) 293, 1111
MvaI CCWGG 2 cut(s) 293, 1111
MvnI CGCG 2 cut(s) 962, 993
MwoI GCNNNNNNNGC 1 cut(s) 449
NdeII GATC 3 cut(s) 565, 621, 831
NlaIII CATG 5 cut(s) 45, 464, 706, 869, 1143
NlaIV GGNNCC 1 cut(s) 578
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 6 cut(s) 296, 417, 556, 813, 979, 988
PkrI GCNGC 4 cut(s) 319, 426, 752, 936
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
PsiI TTATAA 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 1014
Psp6I CCWGG 2 cut(s) 291, 1109
PspGI CCWGG 2 cut(s) 291, 1109
PspN4I GGNNCC 1 cut(s) 578
PstI CTGCAG 1 cut(s) 426
PsuI RGATCY 2 cut(s) 621, 831
PvuII CAGCTG 1 cut(s) 1096
RsaI GTAC 2 cut(s) 228, 1180
RsaNI GTAC 2 cut(s) 227, 1179
RseI CAYNNNNRTG 1 cut(s) 40
SacI GAGCTC 1 cut(s) 1014
SaqAI TTAA 7 cut(s) 126, 479, 561, 689, 1002, 1146, 1242
SatI GCNGC 4 cut(s) 318, 425, 751, 935
Sau3AI GATC 3 cut(s) 565, 621, 831
ScaI AGTACT 1 cut(s) 1180
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 2 cut(s) 293, 1111
SduI GDGCHC 1 cut(s) 1014
SfaNI GCATC 2 cut(s) 174, 483
SfcI CTRYAG 2 cut(s) 422, 1064
SmiMI CAYNNNNRTG 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 252
SmoI CTYRAG 1 cut(s) 252
Sse9I AATT 4 cut(s) 547, 757, 840, 1188
SsiI CCGC 3 cut(s) 318, 962, 993
SspMI CTAG 2 cut(s) 401, 777
SstI GAGCTC 1 cut(s) 1014
StyD4I CCNGG 2 cut(s) 291, 1109
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 2 cut(s) 307, 1068
TaqI TCGA 4 cut(s) 93, 236, 786, 1150
TasI AATT 4 cut(s) 547, 757, 840, 1188
TatI WGTACW 1 cut(s) 1178
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 6 cut(s) 296, 417, 556, 813, 979, 988
Tru1I TTAA 7 cut(s) 126, 479, 561, 689, 1002, 1146, 1242
Tru9I TTAA 7 cut(s) 126, 479, 561, 689, 1002, 1146, 1242
TscAI CASTG 3 cut(s) 210, 459, 1242
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 3 cut(s) 424, 750, 934
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 9 cut(s) 95, 449, 534, 546, 712, 838, 886, 1046, 1128
TspRI CASTG 3 cut(s) 210, 459, 1242
XapI RAATTY 1 cut(s) 1188
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 2 cut(s) 401, 777
ZrmI AGTACT 1 cut(s) 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.