Rh3AG240100

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0011578
Physical Location & Seq
Forward (+)
25127444 .. 25133905
6462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG240100.1

Sequence Viewer

Length: 1884 bp
ATGGCCGACCAGAGAAAAGGTAGCCTTGAAGATACACCAACATGGGCTGTATCAGTATTTGCTTTGTTTTTCTTCGTGCTATCCTTTATAATCGACGATGGTCTTCATCGTCTAACGAAGTTTCTTAAAGGAAGGAGAAGAAAATCCCTGAATAGGGCTTTGAAGAAGTTCAAAACAGAAATGATGCAACTGGGTTTCTTATCACTGCTTCTTACTCTGTCGGAAGTACCAATATCGAATATCTGTGTCTCTCAAGTTTTGGCAAACTCTTTTCTTCCCTGCGAATATCCCTCGGACTCAACTAGTGTCTCATCAGCCGCACAACTTCCAGCTTCAAATACCAACTTCTCTAAGGAAGTCACCACTCAAACTTACTGCGAGGCAAAGGGGTTGGTTTCTCTAGTGTCAAGGGAAGGAATCCTGCAGCTAAACATCTTTATATCGGTCTTGGCAGTGTTTCATGTTCTCTACTGCATCTTAACTATGTTTCTTGGGATGGCCAAGATGAGAAAATGGAATGAATGGGAGGATGAAACTCAGACACTAAATTATCAGATTCTTAATGATCCAAGGAGGTTCCAGCACACCCATCAAACGCCTTTTGGGAAACGACATCTAAAGATCTGGAGTAAACACCCTCTCCTGCTATGGCCTGTTTGTTTTGCTCGGCAGTTTGGCGGCTCAATATCAAAAGTAGATTACATGACTCTGCGAAATGGGTTCATTGTGGCCAACATCAAAGAAGGTAGCAGCTTCAATTTCCTGAACTTCCTTGCTAGAGCTTTCGATGACGATTTTGAGCAAGTAGTTGGAATCAAGTTTTGGATTTGGATCTTTTCAATTCTATTCATTTTTTTCAATGCTCATGTGTTCTACAACTATTATTGGCTACCATTCATTCCCTTACTGATTGTTCTACTCGTGGGAACAAAGCTGCAAGTCGTGATAACTAAAATGTGCGCGGAGAGTTGTAAGAAGAATCCTGTGATTCGCGGAAGCTTTGTGGTGGAGCTCAACAATGATTTATTCTGGTTTGGTCAACCCAATTGGCTTCTTCATCTCCTACAGTTTGTCCTAATCCAGAACTCGTTTCAGCTGGCATTCTTGACCTGGAGTTGGATTGAGTATGGCCAAAGTTCATGCTTTAATCGAAGAACAAAAGATGTTATCATAAAGAGTACTATGGGAATTGTTGTGCAGTTGATTTGTGGTTATGTAACCCTCCCTCTTTATGCACTGGTTAACCAGATGGGGTCTGGCATGAGGAAAGCAGTATTTACTGAGCTCGTAGTTGATGGCCTAAAAAACTGGCACAAGAATGCAAGCCACAGACTATCGAAGAACGGATCCACTACTTCAAGGAAATCATCAAATTCTGCACATTTTTATTCAACAGATGACTCCTCAATTCTAGACAATGAATGTAGCTACAAAACTCCTAATCTAACCATCGTGAGAAGCTCTTCTTCTACCTCCGAGATAATTGAAGAGCAGGTTCCACCCGGTTTAACTCCGAAGAATGGATCCAGTACTTCAAGAAAATCATCAAATTCTGCACAATTTTATTCAACTGATGACTCCTCAATTCCAGACAATGAATGTAGCTACAAAACTCCTCATCCCACCATTGTGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCACGTTTCACCCGGTTTAACTCCAAACCTGGATACTACAAGCTTACCAACTCAAGAAATCACCAGAGACGAAGAGAAACCCAACATCATCAACAAAATAATCTATGACGGTGAGATCTCATTTGGGAGCAGTTGGAAATTAAAGCTGGAGAGCATTAGCAAGAGAAGTGGAGAAATCACTTCAATAATTGAAGAAGAAGTTAACTTCGATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

627

Amino Acids

71.26

Weight (kDa)

8.48

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 464 6.6e-170 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 1483
AccII CGCG 2 cut(s) 962, 993
AciI CCGC 4 cut(s) 318, 678, 962, 993
AclWI GGATC 6 cut(s) 560, 839, 1341, 1354, 1518, 1531
AcoI YGGCCR 4 cut(s) 3, 498, 729, 1129
AcsI RAATTY 2 cut(s) 1372, 1549
AfaI GTAC 3 cut(s) 228, 1180, 1531
AfiI CCNNNNNNNGG 4 cut(s) 153, 154, 1116, 1520
AhlI ACTAGT 1 cut(s) 302
AjnI CCWGG 2 cut(s) 1109, 1695
AjuI GAANNNNNNNTTGG 2 cut(s) 805, 837
AleI CACNNNNGTG 1 cut(s) 1628
Alw21I GWGCWC 3 cut(s) 1014, 1287, 1671
Alw26I GTCTC 3 cut(s) 253, 313, 1728
AlwI GGATC 6 cut(s) 560, 839, 1341, 1354, 1518, 1531
AoxI GGCC 6 cut(s) 3, 498, 650, 729, 1129, 1297
ApeKI GCWGC 3 cut(s) 424, 750, 934
ApoI RAATTY 2 cut(s) 1372, 1549
Asp700I GAANNNNTTC 3 cut(s) 167, 1462, 1639
AspLEI GCGC 1 cut(s) 962
AsuC2I CCSGG 2 cut(s) 1503, 1680
AsuHPI GGTGA 4 cut(s) 352, 1668, 1720, 1790
BalI TGGCCA 3 cut(s) 500, 731, 1131
BamHI GGATCC 2 cut(s) 1346, 1523
BanII GRGCYC 2 cut(s) 1014, 1287
BauI CACGAG 1 cut(s) 920
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 3 cut(s) 1014, 1287, 1671
BbvI GCAGC 3 cut(s) 436, 762, 921
BccI CCATC 6 cut(s) 92, 490, 597, 1243, 1289, 1457
BciT130I CCWGG 2 cut(s) 1111, 1697
BciVI GTATCC 1 cut(s) 1693
BcnI CCSGG 2 cut(s) 1503, 1680
BcoDI GTCTC 3 cut(s) 253, 313, 1728
BcuI ACTAGT 1 cut(s) 302
BfaI CTAG 4 cut(s) 303, 401, 777, 1412
BfmI CTRYAG 2 cut(s) 422, 1064
BfuAI ACCTGC 1 cut(s) 1483
BfuI GTATCC 1 cut(s) 1693
BglII AGATCT 2 cut(s) 621, 1782
BisI GCNGC 5 cut(s) 318, 425, 679, 751, 935
BlsI GCNGC 5 cut(s) 319, 426, 680, 752, 936
BmcAI AGTACT 2 cut(s) 1180, 1531
Bme1390I CCNGG 4 cut(s) 1111, 1503, 1680, 1697
BmiI GGNNCC 4 cut(s) 578, 1348, 1497, 1525
BmrFI CCNGG 4 cut(s) 1111, 1503, 1680, 1697
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 2 cut(s) 174, 483
BmuI ACTGGG 1 cut(s) 200
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 95
BpmI CTGGAG 3 cut(s) 646, 1132, 1835
BpuEI CTTGAG 2 cut(s) 237, 1704
BpuMI CCSGG 2 cut(s) 1503, 1680
BsaBI GATNNNNATC 1 cut(s) 830
BsaJI CCNNGG 2 cut(s) 291, 569
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bsc4I CCNNNNNNNGG 4 cut(s) 153, 154, 1116, 1520
Bse1I ACTGG 4 cut(s) 195, 1242, 1313, 1527
Bse8I GATNNNNATC 1 cut(s) 830
BseBI CCWGG 2 cut(s) 1111, 1697
BseDI CCNNGG 2 cut(s) 291, 569
BseGI GGATG 3 cut(s) 501, 535, 1618
BseJI GATNNNNATC 1 cut(s) 830
BseLI CCNNNNNNNGG 4 cut(s) 153, 154, 1116, 1520
BseMII CTCAG 2 cut(s) 551, 1272
BseNI ACTGG 4 cut(s) 195, 1242, 1313, 1527
BseRI GAGGAG 3 cut(s) 1393, 1570, 1605
BseXI GCAGC 3 cut(s) 436, 762, 921
BsgI GTGCAG 3 cut(s) 1217, 1362, 1539
Bsh1236I CGCG 2 cut(s) 962, 993
BshFI GGCC 6 cut(s) 5, 500, 652, 731, 1131, 1299
BsiHKAI GWGCWC 3 cut(s) 1014, 1287, 1671
BsiSI CCGG 2 cut(s) 1503, 1680
BslI CCNNNNNNNGG 4 cut(s) 153, 154, 1116, 1520
BsmAI GTCTC 3 cut(s) 253, 313, 1728
BsmBI CGTCTC 1 cut(s) 1728
BsmI GAATGC 2 cut(s) 1100, 1324
BsnI GGCC 6 cut(s) 5, 500, 652, 731, 1131, 1299
Bsp1286I GDGCHC 3 cut(s) 1014, 1287, 1671
Bsp143I GATC 7 cut(s) 565, 621, 831, 1346, 1523, 1782, 1876
BspACI CCGC 4 cut(s) 318, 678, 962, 993
BspANI GGCC 6 cut(s) 5, 500, 652, 731, 1131, 1299
BspCNI CTCAG 2 cut(s) 550, 1273
BspFNI CGCG 2 cut(s) 962, 993
BspLI GGNNCC 4 cut(s) 578, 1348, 1497, 1525
BspMAI CTGCAG 1 cut(s) 426
BspMI ACCTGC 1 cut(s) 1483
BspPI GGATC 6 cut(s) 560, 839, 1341, 1354, 1518, 1531
BspQI GCTCTTC 4 cut(s) 1468, 1482, 1645, 1659
BsrI ACTGG 4 cut(s) 195, 1242, 1313, 1527
BssECI CCNNGG 2 cut(s) 291, 569
BssMI GATC 7 cut(s) 565, 621, 831, 1346, 1523, 1782, 1876
BssSI CACGAG 1 cut(s) 920
BssT1I CCWWGG 1 cut(s) 569
Bst2BI CACGAG 1 cut(s) 920
Bst2UI CCWGG 2 cut(s) 1111, 1697
Bst4CI ACNGT 2 cut(s) 1068, 1778
Bst6I CTCTTC 5 cut(s) 1468, 1482, 1645, 1659, 1734
BstC8I GCNNGC 2 cut(s) 1098, 1324
BstDEI CTNAG 3 cut(s) 351, 537, 1281
BstF5I GGATG 3 cut(s) 501, 535, 1618
BstFNI CGCG 2 cut(s) 962, 993
BstHHI GCGC 1 cut(s) 962
BstKTI GATC 7 cut(s) 568, 624, 834, 1349, 1526, 1785, 1879
BstMAI GTCTC 3 cut(s) 253, 313, 1728
BstMBI GATC 7 cut(s) 565, 621, 831, 1346, 1523, 1782, 1876
BstNI CCWGG 2 cut(s) 1111, 1697
BstPAI GACNNNNGTC 1 cut(s) 99
BstSCI CCNGG 4 cut(s) 1109, 1501, 1678, 1695
BstSFI CTRYAG 2 cut(s) 422, 1064
BstUI CGCG 2 cut(s) 962, 993
BstV1I GCAGC 3 cut(s) 436, 762, 921
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 5 cut(s) 621, 831, 1346, 1523, 1782
BstYI RGATCY 5 cut(s) 621, 831, 1346, 1523, 1782
BsuI GTATCC 1 cut(s) 1693
BsuRI GGCC 6 cut(s) 5, 500, 652, 731, 1131, 1299
BtsCI GGATG 3 cut(s) 501, 535, 1618
BtsI GCAGTG 2 cut(s) 203, 459
BtsIMutI CAGTG 3 cut(s) 203, 459, 1235
BveI ACCTGC 1 cut(s) 1483
Cac8I GCNNGC 2 cut(s) 1098, 1324
CfoI GCGC 1 cut(s) 962
Csp6I GTAC 3 cut(s) 227, 1179, 1530
CspCI CAANNNNNGTGG 2 cut(s) 1816, 1851
CviAII CATG 6 cut(s) 42, 461, 703, 866, 1140, 1261
CviQI GTAC 3 cut(s) 227, 1179, 1530
DdeI CTNAG 3 cut(s) 351, 537, 1281
DpnI GATC 7 cut(s) 567, 623, 833, 1348, 1525, 1784, 1878
DpnII GATC 7 cut(s) 565, 621, 831, 1346, 1523, 1782, 1876
EaeI YGGCCR 4 cut(s) 3, 498, 729, 1129
Eam1104I CTCTTC 5 cut(s) 1468, 1482, 1645, 1659, 1734
EarI CTCTTC 5 cut(s) 1468, 1482, 1645, 1659, 1734
Ecl136II GAGCTC 2 cut(s) 1012, 1285
Eco130I CCWWGG 1 cut(s) 569
Eco24I GRGCYC 2 cut(s) 1014, 1287
Eco53kI GAGCTC 2 cut(s) 1012, 1285
EcoICRI GAGCTC 2 cut(s) 1012, 1285
EcoRII CCWGG 2 cut(s) 1109, 1695
EcoT14I CCWWGG 1 cut(s) 569
EcoT38I GRGCYC 2 cut(s) 1014, 1287
ErhI CCWWGG 1 cut(s) 569
Esp3I CGTCTC 1 cut(s) 1728
FaeI CATG 6 cut(s) 45, 464, 706, 869, 1143, 1264
FalI AAGNNNNNCTT 6 cut(s) 9, 41, 1450, 1482, 1627, 1659
FatI CATG 6 cut(s) 41, 460, 702, 865, 1139, 1260
Fnu4HI GCNGC 5 cut(s) 318, 425, 679, 751, 935
FokI GGATG 3 cut(s) 508, 542, 1605
FriOI GRGCYC 2 cut(s) 1014, 1287
Fsp4HI GCNGC 5 cut(s) 318, 425, 679, 751, 935
FspBI CTAG 4 cut(s) 303, 401, 777, 1412
GlaI GCGC 1 cut(s) 961
GluI GCNGC 5 cut(s) 318, 425, 679, 751, 935
GsuI CTGGAG 3 cut(s) 646, 1132, 1835
HaeIII GGCC 6 cut(s) 5, 500, 652, 731, 1131, 1299
HapII CCGG 2 cut(s) 1503, 1680
HhaI GCGC 1 cut(s) 962
Hin1II CATG 6 cut(s) 45, 464, 706, 869, 1143, 1264
Hin6I GCGC 1 cut(s) 960
HinP1I GCGC 1 cut(s) 960
HincII GTYRAC 3 cut(s) 1040, 1243, 1870
HindII GTYRAC 3 cut(s) 1040, 1243, 1870
HindIII AAGCTT 2 cut(s) 997, 1708
HinfI GANTC 9 cut(s) 296, 417, 556, 706, 813, 979, 988, 1400, 1577
HpaI GTTAAC 2 cut(s) 1243, 1870
HpaII CCGG 2 cut(s) 1503, 1680
HphI GGTGA 4 cut(s) 352, 1668, 1720, 1790
Hpy166II GTNNAC 4 cut(s) 632, 1040, 1243, 1870
Hpy188I TCNGA 7 cut(s) 223, 295, 540, 555, 1477, 1515, 1654
Hpy8I GTNNAC 4 cut(s) 632, 1040, 1243, 1870
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 3 cut(s) 126, 407, 737
HpyCH4III ACNGT 2 cut(s) 1068, 1778
HpyCH4IV ACGT 1 cut(s) 1671
HpyCH4V TGCA 9 cut(s) 187, 424, 474, 937, 1198, 1235, 1322, 1379, 1556
HpyF3I CTNAG 3 cut(s) 351, 537, 1281
HpySE526I ACGT 1 cut(s) 1671
Hsp92II CATG 6 cut(s) 45, 464, 706, 869, 1143, 1264
HspAI GCGC 1 cut(s) 960
KspAI GTTAAC 2 cut(s) 1243, 1870
Kzo9I GATC 7 cut(s) 565, 621, 831, 1346, 1523, 1782, 1876
LguI GCTCTTC 4 cut(s) 1468, 1482, 1645, 1659
LmnI GCTCC 2 cut(s) 1009, 1794
Lsp1109I GCAGC 3 cut(s) 436, 762, 921
LweI GCATC 2 cut(s) 174, 483
MaeI CTAG 4 cut(s) 303, 401, 777, 1412
MaeII ACGT 1 cut(s) 1671
MaeIII GTNAC 2 cut(s) 358, 1216
MalI GATC 7 cut(s) 567, 623, 833, 1348, 1525, 1784, 1878
MboI GATC 7 cut(s) 565, 621, 831, 1346, 1523, 1782, 1876
MfeI CAATTG 1 cut(s) 1045
MflI RGATCY 5 cut(s) 621, 831, 1346, 1523, 1782
MhlI GDGCHC 3 cut(s) 1014, 1287, 1671
MlsI TGGCCA 3 cut(s) 500, 731, 1131
MluNI TGGCCA 3 cut(s) 500, 731, 1131
MlyI GAGTC 4 cut(s) 290, 700, 1394, 1571
MmeI TCCRAC 4 cut(s) 201, 790, 1097, 1781
Mox20I TGGCCA 3 cut(s) 500, 731, 1131
MroXI GAANNNNTTC 3 cut(s) 167, 1462, 1639
MscI TGGCCA 3 cut(s) 500, 731, 1131
MseI TTAA 9 cut(s) 126, 479, 561, 1146, 1242, 1508, 1685, 1808, 1869
MslI CAYNNNNRTG 3 cut(s) 40, 1317, 1628
Msp20I TGGCCA 3 cut(s) 500, 731, 1131
MspA1I CMGCKG 1 cut(s) 1096
MspI CCGG 2 cut(s) 1503, 1680
MspR9I CCNGG 4 cut(s) 1111, 1503, 1680, 1697
MunI CAATTG 1 cut(s) 1045
Mva1269I GAATGC 2 cut(s) 1100, 1324
MvaI CCWGG 2 cut(s) 1111, 1697
MvnI CGCG 2 cut(s) 962, 993
NciI CCSGG 2 cut(s) 1503, 1680
NdeII GATC 7 cut(s) 565, 621, 831, 1346, 1523, 1782, 1876
NlaIII CATG 6 cut(s) 45, 464, 706, 869, 1143, 1264
NlaIV GGNNCC 4 cut(s) 578, 1348, 1497, 1525
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 1628
PciSI GCTCTTC 4 cut(s) 1468, 1482, 1645, 1659
PctI GAATGC 2 cut(s) 1100, 1324
PdmI GAANNNNTTC 3 cut(s) 167, 1462, 1639
PfeI GAWTC 5 cut(s) 417, 556, 813, 979, 988
PkrI GCNGC 5 cut(s) 319, 426, 680, 752, 936
PleI GAGTC 4 cut(s) 290, 700, 1394, 1571
PpsI GAGTC 4 cut(s) 290, 700, 1394, 1571
PshAI GACNNNNGTC 1 cut(s) 99
PsiI TTATAA 1 cut(s) 89
Psp124BI GAGCTC 2 cut(s) 1014, 1287
Psp6I CCWGG 2 cut(s) 1109, 1695
PspGI CCWGG 2 cut(s) 1109, 1695
PspN4I GGNNCC 4 cut(s) 578, 1348, 1497, 1525
PstI CTGCAG 1 cut(s) 426
PsuI RGATCY 5 cut(s) 621, 831, 1346, 1523, 1782
PvuII CAGCTG 1 cut(s) 1096
RsaI GTAC 3 cut(s) 228, 1180, 1531
RsaNI GTAC 3 cut(s) 227, 1179, 1530
RseI CAYNNNNRTG 3 cut(s) 40, 1317, 1628
SacI GAGCTC 2 cut(s) 1014, 1287
SapI GCTCTTC 4 cut(s) 1468, 1482, 1645, 1659
SaqAI TTAA 9 cut(s) 126, 479, 561, 1146, 1242, 1508, 1685, 1808, 1869
SatI GCNGC 5 cut(s) 318, 425, 679, 751, 935
Sau3AI GATC 7 cut(s) 565, 621, 831, 1346, 1523, 1782, 1876
ScaI AGTACT 2 cut(s) 1180, 1531
SchI GAGTC 4 cut(s) 290, 700, 1394, 1571
ScrFI CCNGG 4 cut(s) 1111, 1503, 1680, 1697
SduI GDGCHC 3 cut(s) 1014, 1287, 1671
SfaNI GCATC 2 cut(s) 174, 483
SfcI CTRYAG 2 cut(s) 422, 1064
SmiMI CAYNNNNRTG 3 cut(s) 40, 1317, 1628
SmlI CTYRAG 2 cut(s) 252, 1719
SmoI CTYRAG 2 cut(s) 252, 1719
SpeI ACTAGT 1 cut(s) 302
SsiI CCGC 4 cut(s) 318, 678, 962, 993
SspMI CTAG 4 cut(s) 303, 401, 777, 1412
SstI GAGCTC 2 cut(s) 1014, 1287
StyD4I CCNGG 4 cut(s) 1109, 1501, 1678, 1695
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 2 cut(s) 1068, 1778
TaiI ACGT 1 cut(s) 1674
TaqI TCGA 6 cut(s) 93, 236, 786, 1150, 1337, 1875
TaqII GACCGA 1 cut(s) 433
TatI WGTACW 2 cut(s) 1178, 1529
TauI GCSGC 2 cut(s) 320, 681
TfiI GAWTC 5 cut(s) 417, 556, 813, 979, 988
Tru1I TTAA 9 cut(s) 126, 479, 561, 1146, 1242, 1508, 1685, 1808, 1869
Tru9I TTAA 9 cut(s) 126, 479, 561, 1146, 1242, 1508, 1685, 1808, 1869
TscAI CASTG 3 cut(s) 210, 459, 1242
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 3 cut(s) 424, 750, 934
Tsp45I GTSAC 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 1359
TspRI CASTG 3 cut(s) 210, 459, 1242
XapI RAATTY 2 cut(s) 1372, 1549
XbaI TCTAGA 1 cut(s) 1411
XcmI CCANNNNNNNNNTGG 1 cut(s) 1253
XmnI GAANNNNTTC 3 cut(s) 167, 1462, 1639
XspI CTAG 4 cut(s) 303, 401, 777, 1412
ZrmI AGTACT 2 cut(s) 1180, 1531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.