Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011578

Physical Location & Seq

Genomic Coordinates Reverse (-)

10820115 .. 10826478

Gene Structure 6364 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1140400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCACGTTCCCTTGAAGAAACGCCAACATGGGCTGTTTCAGTATTTGCTTTGATTTTCTTCTTCCTATCCTTCCTCATCGACACTATTCTTCACCTTTTAACAAAGTGGAAGTTCGTGCTAAGACGCACAATAACCATTTACTTTGTTCTTAATTCATTTTCTTGTACTTTAAATATAGAAATGATGAAACTGGGTCTCATGTCACTGCTTCTAACGATATCAGAAGAACCAATATCACAAATCTGCGTTGGCCAAGCTGTGGCAAACACTTTTCTTCTATGCAAATATCCCGTTAACTCCTTAGACGTAGAACCTTCTGTCTCATCAGCCACGCAAGTCCCACGCTCAAATTCCACCAATCGCTCTAAGGAAGTCAACGATCAAAACTATTGCGAGGCAAAGGGGATGGTTCCTTTATTGCCAAGGGCAGGAGTCTTGCAGCTGAACATCTTAATCTCTCTGGTGACAGTTTTTCATGTGCTCTACTGCATCTTAACCATGTTTCTCGGGATGGCCAAGATGGGGAAATGGAATGCATGGGAGAATGAGACTCGAACACTTGAATATCAGATCCTTAATGGTAACTCTTTGCCTCTGTTCTGTAAGACTGTATATAATAGTTACTCAGTATATATGTATTTACGCTTCACGCTTCTGAGGAAATTTTGTTATGTATATACAGATTCAAGGAGGTTCCAACTCATCCACCAAACTCCTTTTGGGAAAAGACATTTAAAGTTCTGGAGTAAACATCCTCTGCTGCTTTGGCCTGTTTGTTTTGCTCGACAGTTCTGTGGTTCAATCTCAAAAACTGATTACATGACTCTGAGGAATGGTTTCATTAGGGCCAACTTTGCAGACGGCAACAACTTCAATTTCCTGAGATTCCTTGCAAGAGCTTTCGATGACGACTTTGAGCAAGTGGTTGGCATTAGATTTTGGATTTGGCTCTTCTCTATCCTTTTCATATTTCTCAGTTCGCATGTGTTTTATGATCATTATTGGCTGCCATTCATTCCCTTACTGATCGTGATAGTCGTGGGAGCAAAGCTGGAGGTGATAATAACTAAAATGTGTGTGGAGAGCTGCAAGAATACTTCCGTTGTTCGAGGACGTGTTTTAGTGCAGCTCAATGATGACTTGTTTTGGTTTGGTCGACCCAGATGGCTTCTTCACCTCATCCAACTCGTCCTAATCCAGTTTGAGTCTGGTTCAGGGTCTTGCTTAAATCGCAAAAGTGAAATCATAGCCATAAGGATTACCATGGGAGTGGTTGTGCAGTTAATTTGTGGTTATGTGACCCTGCCTCTCCATGTCCTTGTTACCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mlo

Protein Analysis

445

Amino Acids

51.42

Weight (kDa)

9.23

Isoelectric Point (pI)

42.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mlo	PF03094	3 - 196	2.3e-43	Mlo family
Mlo	PF03094	227 - 444	8.4e-76	Mlo family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011578)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g23220
malus_domestica	MD13G1140400.v1.1
prunus_persica	Prupe.3G124700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0481051
rosa_laevigata	RLG00000023425 RLG00000023429
rosa_multiflora	Rmu_sc0001551.1_g000031 Rmu_sc0001551.1_g000036
rosa_rugosa	Rorug03G0189400 Rorug03G0189900
rosa_samantha	Rh3AG240100 Rh3BG275300 Rh3BG275900 Rh3CG271000 Rh3CG271200 Rh3DG266900 Rh3DG267100 Rh3DG267200
rosa_wichuraiana	Rw3G021440 Rw3G021450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	262
AccI	GTMKAC	1 cut(s)	1159
AclWI	GGATC	1 cut(s)	568
AcoI	YGGCCR	2 cut(s)	253, 516
AcsI	RAATTY	2 cut(s)	352, 665
AfaI	GTAC	1 cut(s)	169
AfiI	CCNNNNNNNGG	3 cut(s)	262, 431, 525
AflIII	ACRYGT	1 cut(s)	1117
AgsI	TTSAA	5 cut(s)	17, 566, 690, 804, 877
AjiI	CACGTC	1 cut(s)	1118
AluBI	AGCT	6 cut(s)	260, 445, 902, 1054, 1089, 1132
AluI	AGCT	6 cut(s)	260, 445, 902, 1054, 1089, 1132
Alw21I	GWGCWC	1 cut(s)	486
Alw26I	GTCTC	3 cut(s)	203, 328, 545
AlwI	GGATC	1 cut(s)	568
Ama87I	CYCGRG	1 cut(s)	509
AoxI	GGCC	4 cut(s)	253, 516, 770, 849
ApeKI	GCWGC	5 cut(s)	442, 763, 1009, 1089, 1129
ApoI	RAATTY	2 cut(s)	352, 665
Asp700I	GAANNNNTTC	1 cut(s)	839
AspS9I	GGNCC	1 cut(s)	849
AsuHPI	GGTGA	4 cut(s)	86, 478, 1072, 1169
AvaI	CYCGRG	1 cut(s)	509
BalI	TGGCCA	2 cut(s)	255, 518
BarI	GAAGNNNNNNTAC	2 cut(s)	632, 664
Bbv12I	GWGCWC	1 cut(s)	486
BbvI	GCAGC	5 cut(s)	454, 750, 996, 1076, 1141
BccI	CCATC	4 cut(s)	403, 508, 517, 1161
BceAI	ACGGC	1 cut(s)	880
BclI	TGATCA	1 cut(s)	997
BcoDI	GTCTC	3 cut(s)	203, 328, 545
BisI	GCNGC	5 cut(s)	443, 764, 1010, 1090, 1130
BlsI	GCNGC	5 cut(s)	444, 765, 1011, 1091, 1131
BmeT110I	CYCGRG	1 cut(s)	509
BmgBI	CACGTC	1 cut(s)	1118
BmgT120I	GGNCC	1 cut(s)	849
BmiI	GGNNCC	2 cut(s)	414, 698
BmrI	ACTGGG	2 cut(s)	203, 1324
BmsI	GCATC	1 cut(s)	501
BmuI	ACTGGG	2 cut(s)	203, 1324
BpmI	CTGGAG	2 cut(s)	766, 1076
BsaI	GGTCTC	1 cut(s)	203
BsaJI	CCNNGG	2 cut(s)	425, 1266
BsaXI	ACNNNNNCTCC	2 cut(s)	1263, 1293
Bsc4I	CCNNNNNNNGG	3 cut(s)	262, 431, 525
Bse1I	ACTGG	3 cut(s)	198, 1201, 1330
BseDI	CCNNGG	2 cut(s)	425, 1266
BseGI	GGATG	5 cut(s)	414, 519, 705, 754, 1182
BseLI	CCNNNNNNNGG	3 cut(s)	262, 431, 525
BseMII	CTCAG	5 cut(s)	642, 650, 821, 875, 991
BseNI	ACTGG	3 cut(s)	198, 1201, 1330
BseXI	GCAGC	5 cut(s)	454, 750, 996, 1076, 1141
BsgI	GTGCAG	2 cut(s)	1148, 1301
BshFI	GGCC	4 cut(s)	255, 518, 772, 851
BsiHKAI	GWGCWC	1 cut(s)	486
BsiHKCI	CYCGRG	1 cut(s)	509
BslFI	GGGAC	1 cut(s)	326
BslI	CCNNNNNNNGG	3 cut(s)	262, 431, 525
BsmAI	GTCTC	3 cut(s)	203, 328, 545
BsmFI	GGGAC	1 cut(s)	326
BsmI	GAATGC	1 cut(s)	541
BsnI	GGCC	4 cut(s)	255, 518, 772, 851
Bso31I	GGTCTC	1 cut(s)	203
BsoBI	CYCGRG	1 cut(s)	509
Bsp1286I	GDGCHC	1 cut(s)	486
Bsp143I	GATC	4 cut(s)	382, 573, 997, 1029
Bsp19I	CCATGG	1 cut(s)	1266
BspANI	GGCC	4 cut(s)	255, 518, 772, 851
BspCNI	CTCAG	5 cut(s)	641, 651, 822, 876, 990
BspLI	GGNNCC	2 cut(s)	414, 698
BspPI	GGATC	1 cut(s)	568
BspQI	GCTCTTC	1 cut(s)	959
BspTNI	GGTCTC	1 cut(s)	203
BsrI	ACTGG	3 cut(s)	198, 1201, 1330
BssECI	CCNNGG	2 cut(s)	425, 1266
BssMI	GATC	4 cut(s)	382, 573, 997, 1029
BssT1I	CCWWGG	2 cut(s)	425, 1266
Bst4CI	ACNGT	3 cut(s)	472, 613, 792
Bst6I	CTCTTC	1 cut(s)	959
BstDEI	CTNAG	8 cut(s)	122, 304, 369, 628, 659, 830, 884, 977
BstDSI	CCRYGG	1 cut(s)	1266
BstF5I	GGATG	5 cut(s)	414, 519, 705, 754, 1182
BstKTI	GATC	4 cut(s)	385, 576, 1000, 1032
BstMAI	GTCTC	3 cut(s)	203, 328, 545
BstMBI	GATC	4 cut(s)	382, 573, 997, 1029
BstMWI	GCNNNNNNNGC	3 cut(s)	769, 857, 1233
BstNSI	RCATGY	1 cut(s)	989
BstV1I	GCAGC	5 cut(s)	454, 750, 996, 1076, 1141
BstX2I	RGATCY	1 cut(s)	573
BstXI	CCANNNNNNTGG	1 cut(s)	1273
BstYI	RGATCY	1 cut(s)	573
BsuRI	GGCC	4 cut(s)	255, 518, 772, 851
BtgI	CCRYGG	1 cut(s)	1266
BtrI	CACGTC	1 cut(s)	1118
BtsCI	GGATG	5 cut(s)	414, 519, 705, 754, 1182
BtsI	GCAGTG	1 cut(s)	206
BtsIMutI	CAGTG	1 cut(s)	206
Cfr13I	GGNCC	1 cut(s)	849
CseI	GACGC	1 cut(s)	135
Csp6I	GTAC	1 cut(s)	168
CviAII	CATG	9 cut(s)	30, 202, 479, 502, 540, 823, 986, 1267, 1316
CviQI	GTAC	1 cut(s)	168
DdeI	CTNAG	8 cut(s)	122, 304, 369, 628, 659, 830, 884, 977
DpnI	GATC	4 cut(s)	384, 575, 999, 1031
DpnII	GATC	4 cut(s)	382, 573, 997, 1029
DraI	TTTAAA	2 cut(s)	174, 738
EaeI	YGGCCR	2 cut(s)	253, 516
Eam1104I	CTCTTC	1 cut(s)	959
EarI	CTCTTC	1 cut(s)	959
Eco130I	CCWWGG	2 cut(s)	425, 1266
Eco31I	GGTCTC	1 cut(s)	203
Eco32I	GATATC	1 cut(s)	222
Eco88I	CYCGRG	1 cut(s)	509
EcoRV	GATATC	1 cut(s)	222
EcoT14I	CCWWGG	2 cut(s)	425, 1266
EcoT22I	ATGCAT	1 cut(s)	541
ErhI	CCWWGG	2 cut(s)	425, 1266
FaeI	CATG	9 cut(s)	33, 205, 482, 505, 543, 826, 989, 1270, 1319
FaqI	GGGAC	1 cut(s)	326
FatI	CATG	9 cut(s)	29, 201, 478, 501, 539, 822, 985, 1266, 1315
FbaI	TGATCA	1 cut(s)	997
FblI	GTMKAC	1 cut(s)	1159
Fnu4HI	GCNGC	5 cut(s)	443, 764, 1010, 1090, 1130
FokI	GGATG	5 cut(s)	421, 526, 692, 741, 1169
Fsp4HI	GCNGC	5 cut(s)	443, 764, 1010, 1090, 1130
GluI	GCNGC	5 cut(s)	443, 764, 1010, 1090, 1130
GsuI	CTGGAG	2 cut(s)	766, 1076
HaeIII	GGCC	4 cut(s)	255, 518, 772, 851
HgaI	GACGC	1 cut(s)	135
Hin1II	CATG	9 cut(s)	33, 205, 482, 505, 543, 826, 989, 1270, 1319
HincII	GTYRAC	3 cut(s)	298, 379, 1160
HindII	GTYRAC	3 cut(s)	298, 379, 1160
HinfI	GANTC	6 cut(s)	435, 553, 686, 826, 888, 1208
HpaI	GTTAAC	1 cut(s)	298
HphI	GGTGA	4 cut(s)	86, 478, 1072, 1169
Hpy166II	GTNNAC	4 cut(s)	298, 379, 752, 1160
Hpy188I	TCNGA	4 cut(s)	226, 573, 660, 831
Hpy188III	TCNNGA	4 cut(s)	511, 745, 883, 1033
Hpy8I	GTNNAC	4 cut(s)	298, 379, 752, 1160
HpyAV	CCTTC	2 cut(s)	82, 327
HpyCH4III	ACNGT	3 cut(s)	472, 613, 792
HpyCH4IV	ACGT	3 cut(s)	7, 309, 1117
HpyCH4V	TGCA	9 cut(s)	285, 442, 492, 539, 860, 896, 1092, 1129, 1282
HpyF10VI	GCNNNNNNNGC	3 cut(s)	769, 857, 1233
HpyF3I	CTNAG	8 cut(s)	122, 304, 369, 628, 659, 830, 884, 977
HpySE526I	ACGT	3 cut(s)	7, 309, 1117
Hsp92II	CATG	9 cut(s)	33, 205, 482, 505, 543, 826, 989, 1270, 1319
Ksp22I	TGATCA	1 cut(s)	997
KspAI	GTTAAC	1 cut(s)	298
Kzo9I	GATC	4 cut(s)	382, 573, 997, 1029
LguI	GCTCTTC	1 cut(s)	959
LmnI	GCTCC	1 cut(s)	1046
Lsp1109I	GCAGC	5 cut(s)	454, 750, 996, 1076, 1141
LweI	GCATC	1 cut(s)	501
MaeII	ACGT	3 cut(s)	7, 309, 1117
MaeIII	GTNAC	6 cut(s)	204, 466, 584, 623, 1300, 1324
MalI	GATC	4 cut(s)	384, 575, 999, 1031
MboI	GATC	4 cut(s)	382, 573, 997, 1029
MboII	GAAGA	8 cut(s)	29, 52, 55, 83, 239, 269, 946, 1166
MflI	RGATCY	1 cut(s)	573
MhlI	GDGCHC	1 cut(s)	486
MlsI	TGGCCA	2 cut(s)	255, 518
MluCI	AATT	5 cut(s)	154, 352, 665, 877, 1287
MluNI	TGGCCA	2 cut(s)	255, 518
MlyI	GAGTC	4 cut(s)	444, 547, 820, 1217
MmeI	TCCRAC	2 cut(s)	724, 1210
Mox20I	TGGCCA	2 cut(s)	255, 518
Mph1103I	ATGCAT	1 cut(s)	541
MroXI	GAANNNNTTC	1 cut(s)	839
MscI	TGGCCA	2 cut(s)	255, 518
MslI	CAYNNNNRTG	1 cut(s)	1271
Msp20I	TGGCCA	2 cut(s)	255, 518
MspA1I	CMGCKG	1 cut(s)	445
Mva1269I	GAATGC	1 cut(s)	541
MwoI	GCNNNNNNNGC	3 cut(s)	769, 857, 1233
NcoI	CCATGG	1 cut(s)	1266
NdeII	GATC	4 cut(s)	382, 573, 997, 1029
NlaIII	CATG	9 cut(s)	33, 205, 482, 505, 543, 826, 989, 1270, 1319
NlaIV	GGNNCC	2 cut(s)	414, 698
NmuCI	GTSAC	3 cut(s)	204, 466, 1300
NsiI	ATGCAT	1 cut(s)	541
NspI	RCATGY	1 cut(s)	989
PciSI	GCTCTTC	1 cut(s)	959
PcsI	WCGNNNNNNNCGW	1 cut(s)	1038
PctI	GAATGC	1 cut(s)	541
PdmI	GAANNNNTTC	1 cut(s)	839
PfeI	GAWTC	2 cut(s)	686, 888
PflMI	CCANNNNNTGG	1 cut(s)	262
PkrI	GCNGC	5 cut(s)	444, 765, 1011, 1091, 1131
PleI	GAGTC	4 cut(s)	443, 547, 820, 1216
PpsI	GAGTC	4 cut(s)	443, 547, 820, 1216
PspN4I	GGNNCC	2 cut(s)	414, 698
PspPI	GGNCC	1 cut(s)	849
PsuI	RGATCY	1 cut(s)	573
PvuII	CAGCTG	1 cut(s)	445
RsaI	GTAC	1 cut(s)	169
RsaNI	GTAC	1 cut(s)	168
RseI	CAYNNNNRTG	1 cut(s)	1271
SalI	GTCGAC	1 cut(s)	1158
SapI	GCTCTTC	1 cut(s)	959
SatI	GCNGC	5 cut(s)	443, 764, 1010, 1090, 1130
Sau3AI	GATC	4 cut(s)	382, 573, 997, 1029
Sau96I	GGNCC	1 cut(s)	849
SchI	GAGTC	4 cut(s)	444, 547, 820, 1217
SduI	GDGCHC	1 cut(s)	486
SfaNI	GCATC	1 cut(s)	501
SmiMI	CAYNNNNRTG	1 cut(s)	1271
Sse9I	AATT	5 cut(s)	154, 352, 665, 877, 1287
StyI	CCWWGG	2 cut(s)	425, 1266
TaaI	ACNGT	3 cut(s)	472, 613, 792
TaiI	ACGT	3 cut(s)	10, 312, 1120
TaqI	TCGA	6 cut(s)	81, 556, 787, 906, 1111, 1159
TasI	AATT	5 cut(s)	154, 352, 665, 877, 1287
TatI	WGTACW	1 cut(s)	167
TfiI	GAWTC	2 cut(s)	686, 888
TscAI	CASTG	1 cut(s)	213
TseFI	GTSAC	3 cut(s)	204, 466, 1300
TseI	GCWGC	5 cut(s)	442, 763, 1009, 1089, 1129
Tsp45I	GTSAC	3 cut(s)	204, 466, 1300
TspDTI	ATGAA	6 cut(s)	147, 203, 467, 832, 958, 1006
TspGWI	ACGGA	1 cut(s)	1093
TspRI	CASTG	1 cut(s)	213
Van91I	CCANNNNNTGG	1 cut(s)	262
XapI	RAATTY	2 cut(s)	352, 665
XceI	RCATGY	1 cut(s)	989
XcmI	CCANNNNNNNNNTGG	2 cut(s)	719, 1208
XmiI	GTMKAC	1 cut(s)	1159
XmnI	GAANNNNTTC	1 cut(s)	839
Zsp2I	ATGCAT	1 cut(s)	541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.