Rh3BG275900

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011578
Physical Location & Seq
Forward (+)
27022961 .. 27028475
5515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG275900.1

Sequence Viewer

Length: 1398 bp
ATGATGCAACTGGGTTTCGTATCTTTGCTTCTTACTCTGTCCGAAGTACCAATATCGAATATCTGTGTCTCTCAAGTTTTGGCAAACTCTTTTCTTCCCTGCGAATATCCCTCGGACTCAACTAGTGTCTCATCAGCCGCACAAGTTCCAGCTTCAAATACCAACTTCTCTGAAGTCACCACTCAAAATTACTGCGAGGCAAAGGCATGTTTCGCTCGGCAGTTCGGTGGCTCAATCTCAAAAGTCGATTACATGACTCTGCGAAATGGGTTCATTGTGGCCAACTGCACAGAAGGTAGCAACTTCAACTTCCTGAACTTCCTTGCCAGAGCTTTCGACGACGATTTTGAGCAAGTGGTTGGAATCAAGCTTTGGATCTGGATCTCTTCAACTCTTTTCATGTTTTTCAATGCCCACGTGTTCTATAACTATTACTGGCTACCATTCATTCCCCTACTGATTGTTTTAGTAGTGGGAACGAAGCTGCAAGTCGTGATAACTAACATGTGCGTCGAGAGTTATGAGAAGAATCCTGTGATTCGAGGAAGCTTTGTTGTGAAGCTCAAAGATGGCTTATTTTGGTTCAGTCAACCCAATTGGCTTCTTCATCTTCTCCAATTTGTCCTAATCCAGAACTCGTTTCAGCTGGCATTCTTGACCTGGACATGGTTTGAATTTGGCCAGAATTCATGCTTTAATCGAAGAACAAAAGATATCGTCATTAAGAGTAGTATGGGACTGGGTGTGCAGTTGATATGTGGTTATGTGACCCTCCCTCTTTACGCACTGGTTACCCAGATGGGGTCTGGCATGAGGAAAGCAGTATTTACTGAGCTCGTAGTTGATGGCCTCAAAAACTGGCAAAAGAATGCAAGACGCAGACTATCGAAGAATGGATCCACTACTTCAAGGAAATCATCAAATTCTGCACATTTTTATTCAACCGATGACTCCTCATTTACAGACAATGAATGTAGCTACAAAACTCCTAATCCCACCATAGCGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCGCGTTCCAGCCGGTTTAAGTCCGAAGAATGGATCCACTACTTCAAGGAAATCCTCCGATTCTGCACATTTTTATTCAACTGATAACTCCTCATTTCCAGACAATGAATGTAGCAACAAAAATCCTAATCCCACCATAGTGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCAAGTTCCACCCGGTTTAACTCCGAACCCGGATACTACAAGTTTACCAACTCCAGAAATCACCAGAGAGGAACAGAATCCCAACATCATCAACAAAATAATCTATGACGGTGAGATTTCTTTTGGGAGCAGTTGGAAATTAAAGCTGGAGAGCATGAGCAAGAGAAGTGGGGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

465

Amino Acids

52.03

Weight (kDa)

6.47

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 1 - 45 6.6e-08 Mlo family
Mlo PF03094 63 - 312 4.4e-96 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1044
AciI CCGC 1 cut(s) 138
AclWI GGATC 6 cut(s) 383, 389, 891, 904, 1068, 1081
AcoI YGGCCR 2 cut(s) 279, 679
AcsI RAATTY 4 cut(s) 674, 685, 922, 1391
AcuI CTGAAG 1 cut(s) 192
AcvI CACGTG 1 cut(s) 418
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 3 cut(s) 666, 801, 1070
AflIII ACRYGT 2 cut(s) 417, 504
AhlI ACTAGT 1 cut(s) 122
AjnI CCWGG 1 cut(s) 659
AjuI GAANNNNNNNTTGG 2 cut(s) 355, 387
AleI CACNNNNGTG 1 cut(s) 1178
Alw21I GWGCWC 1 cut(s) 837
Alw26I GTCTC 2 cut(s) 73, 133
AlwI GGATC 6 cut(s) 383, 389, 891, 904, 1068, 1081
AoxI GGCC 3 cut(s) 279, 679, 847
ApeKI GCWGC 1 cut(s) 484
ApoI RAATTY 4 cut(s) 674, 685, 922, 1391
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
Asp700I GAANNNNTTC 2 cut(s) 1012, 1189
AspLEI GCGC 1 cut(s) 1044
AsuC2I CCSGG 2 cut(s) 1230, 1247
AsuHPI GGTGA 3 cut(s) 169, 1270, 1340
BalI TGGCCA 2 cut(s) 281, 681
BamHI GGATCC 2 cut(s) 896, 1073
BanII GRGCYC 1 cut(s) 837
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbrPI CACGTG 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 837
BbvI GCAGC 1 cut(s) 471
BccI CCATC 3 cut(s) 563, 793, 839
BciT130I CCWGG 1 cut(s) 661
BciVI GTATCC 1 cut(s) 1243
BcnI CCSGG 2 cut(s) 1230, 1247
BcoDI GTCTC 2 cut(s) 73, 133
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 1 cut(s) 123
BfuI GTATCC 1 cut(s) 1243
BisI GCNGC 2 cut(s) 138, 485
BlsI GCNGC 2 cut(s) 139, 486
Bme1390I CCNGG 3 cut(s) 661, 1230, 1247
BmiI GGNNCC 2 cut(s) 898, 1075
BmrFI CCNGG 3 cut(s) 661, 1230, 1247
BmrI ACTGGG 2 cut(s) 20, 749
BmuI ACTGGG 2 cut(s) 20, 749
BpmI CTGGAG 2 cut(s) 1254, 1385
BpuEI CTTGAG 1 cut(s) 57
BpuMI CCSGG 2 cut(s) 1230, 1247
BsaAI YACGTR 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 3 cut(s) 666, 801, 1070
Bse118I RCCGGY 1 cut(s) 1052
Bse1I ACTGG 5 cut(s) 15, 440, 744, 792, 863
Bse8I GATNNNNATC 1 cut(s) 380
BseBI CCWGG 1 cut(s) 661
BseDI CCNNGG 1 cut(s) 111
BseJI GATNNNNATC 1 cut(s) 380
BseLI CCNNNNNNNGG 3 cut(s) 666, 801, 1070
BseMII CTCAG 1 cut(s) 822
BseNI ACTGG 5 cut(s) 15, 440, 744, 792, 863
BseRI GAGGAG 2 cut(s) 943, 1120
BseXI GCAGC 1 cut(s) 471
BsgI GTGCAG 4 cut(s) 271, 767, 912, 1089
Bsh1236I CGCG 1 cut(s) 1044
BshFI GGCC 3 cut(s) 281, 681, 849
BsiHKAI GWGCWC 1 cut(s) 837
BsiSI CCGG 3 cut(s) 1053, 1230, 1247
BslFI GGGAC 1 cut(s) 750
BslI CCNNNNNNNGG 3 cut(s) 666, 801, 1070
BsmAI GTCTC 2 cut(s) 73, 133
BsmFI GGGAC 1 cut(s) 750
BsmI GAATGC 2 cut(s) 650, 874
BsnI GGCC 3 cut(s) 281, 681, 849
Bsp1286I GDGCHC 1 cut(s) 837
Bsp143I GATC 4 cut(s) 375, 381, 896, 1073
BspACI CCGC 1 cut(s) 138
BspANI GGCC 3 cut(s) 281, 681, 849
BspCNI CTCAG 1 cut(s) 823
BspFNI CGCG 1 cut(s) 1044
BspLI GGNNCC 2 cut(s) 898, 1075
BspPI GGATC 6 cut(s) 383, 389, 891, 904, 1068, 1081
BspQI GCTCTTC 4 cut(s) 1018, 1032, 1195, 1209
BsrFI RCCGGY 1 cut(s) 1052
BsrI ACTGG 5 cut(s) 15, 440, 744, 792, 863
BssAI RCCGGY 1 cut(s) 1052
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 4 cut(s) 375, 381, 896, 1073
Bst2UI CCWGG 1 cut(s) 661
Bst4CI ACNGT 1 cut(s) 1328
Bst6I CTCTTC 5 cut(s) 391, 1018, 1032, 1195, 1209
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 1 cut(s) 831
BstEII GGTNACC 1 cut(s) 790
BstFNI CGCG 1 cut(s) 1044
BstHHI GCGC 1 cut(s) 1044
BstKTI GATC 4 cut(s) 378, 384, 899, 1076
BstMAI GTCTC 2 cut(s) 73, 133
BstMBI GATC 4 cut(s) 375, 381, 896, 1073
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNI CCWGG 1 cut(s) 661
BstNSI RCATGY 2 cut(s) 210, 508
BstPI GGTNACC 1 cut(s) 790
BstSCI CCNGG 3 cut(s) 659, 1228, 1245
BstUI CGCG 1 cut(s) 1044
BstV1I GCAGC 1 cut(s) 471
BstX2I RGATCY 4 cut(s) 375, 381, 896, 1073
BstYI RGATCY 4 cut(s) 375, 381, 896, 1073
BsuI GTATCC 1 cut(s) 1243
BsuRI GGCC 3 cut(s) 281, 681, 849
BtsIMutI CAGTG 1 cut(s) 785
Cac8I GCNNGC 1 cut(s) 648
CfoI GCGC 1 cut(s) 1044
Cfr10I RCCGGY 1 cut(s) 1052
CseI GACGC 2 cut(s) 499, 885
Csp6I GTAC 1 cut(s) 47
CspCI CAANNNNNGTGG 1 cut(s) 1366
CviAII CATG 8 cut(s) 207, 253, 400, 505, 666, 690, 811, 1372
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 1 cut(s) 831
DpnI GATC 4 cut(s) 377, 383, 898, 1075
DpnII GATC 4 cut(s) 375, 381, 896, 1073
EaeI YGGCCR 2 cut(s) 279, 679
Eam1104I CTCTTC 5 cut(s) 391, 1018, 1032, 1195, 1209
EarI CTCTTC 5 cut(s) 391, 1018, 1032, 1195, 1209
Ecl136II GAGCTC 1 cut(s) 835
Eco24I GRGCYC 1 cut(s) 837
Eco32I GATATC 1 cut(s) 715
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 192
Eco72I CACGTG 1 cut(s) 418
Eco91I GGTNACC 1 cut(s) 790
EcoICRI GAGCTC 1 cut(s) 835
EcoO65I GGTNACC 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 1 cut(s) 659
EcoRV GATATC 1 cut(s) 715
EcoT38I GRGCYC 1 cut(s) 837
FaeI CATG 8 cut(s) 210, 256, 403, 508, 669, 693, 814, 1375
FalI AAGNNNNNCTT 4 cut(s) 1000, 1032, 1177, 1209
FaqI GGGAC 1 cut(s) 750
FatI CATG 8 cut(s) 206, 252, 399, 504, 665, 689, 810, 1371
Fnu4HI GCNGC 2 cut(s) 138, 485
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 2 cut(s) 138, 485
FspBI CTAG 1 cut(s) 123
GlaI GCGC 1 cut(s) 1043
GluI GCNGC 2 cut(s) 138, 485
GsuI CTGGAG 2 cut(s) 1254, 1385
HaeIII GGCC 3 cut(s) 281, 681, 849
HapII CCGG 3 cut(s) 1053, 1230, 1247
HgaI GACGC 2 cut(s) 499, 885
HhaI GCGC 1 cut(s) 1044
Hin1II CATG 8 cut(s) 210, 256, 403, 508, 669, 693, 814, 1375
Hin6I GCGC 1 cut(s) 1042
HinP1I GCGC 1 cut(s) 1042
HincII GTYRAC 1 cut(s) 590
HindII GTYRAC 1 cut(s) 590
HindIII AAGCTT 2 cut(s) 368, 547
HinfI GANTC 8 cut(s) 116, 256, 363, 529, 538, 950, 1100, 1294
HpaII CCGG 3 cut(s) 1053, 1230, 1247
HphI GGTGA 3 cut(s) 169, 1270, 1340
Hpy166II GTNNAC 2 cut(s) 590, 1262
Hpy188I TCNGA 8 cut(s) 43, 115, 172, 1027, 1065, 1099, 1204, 1242
Hpy188III TCNNGA 8 cut(s) 313, 379, 493, 514, 631, 655, 1139, 1271
Hpy8I GTNNAC 2 cut(s) 590, 1262
Hpy99I CGWCG 3 cut(s) 341, 344, 515
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 1 cut(s) 1328
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 7 cut(s) 7, 288, 487, 748, 872, 929, 1106
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 1 cut(s) 831
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 8 cut(s) 210, 256, 403, 508, 669, 693, 814, 1375
HspAI GCGC 1 cut(s) 1042
Kzo9I GATC 4 cut(s) 375, 381, 896, 1073
LguI GCTCTTC 4 cut(s) 1018, 1032, 1195, 1209
LmnI GCTCC 1 cut(s) 1344
Lsp1109I GCAGC 1 cut(s) 471
MaeI CTAG 1 cut(s) 123
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 3 cut(s) 175, 766, 790
MalI GATC 4 cut(s) 377, 383, 898, 1075
MboI GATC 4 cut(s) 375, 381, 896, 1073
MfeI CAATTG 1 cut(s) 595
MflI RGATCY 4 cut(s) 375, 381, 896, 1073
MhlI GDGCHC 1 cut(s) 837
MlsI TGGCCA 2 cut(s) 281, 681
MluNI TGGCCA 2 cut(s) 281, 681
MlyI GAGTC 3 cut(s) 110, 250, 944
MmeI TCCRAC 2 cut(s) 340, 1331
Mox20I TGGCCA 2 cut(s) 281, 681
MroXI GAANNNNTTC 2 cut(s) 1012, 1189
MscI TGGCCA 2 cut(s) 281, 681
MseI TTAA 5 cut(s) 696, 723, 1058, 1235, 1358
MslI CAYNNNNRTG 1 cut(s) 1178
Msp20I TGGCCA 2 cut(s) 281, 681
MspA1I CMGCKG 1 cut(s) 646
MspI CCGG 3 cut(s) 1053, 1230, 1247
MspR9I CCNGG 3 cut(s) 661, 1230, 1247
MunI CAATTG 1 cut(s) 595
Mva1269I GAATGC 2 cut(s) 650, 874
MvaI CCWGG 1 cut(s) 661
MvnI CGCG 1 cut(s) 1044
MwoI GCNNNNNNNGC 1 cut(s) 212
NciI CCSGG 2 cut(s) 1230, 1247
NdeII GATC 4 cut(s) 375, 381, 896, 1073
NlaIII CATG 8 cut(s) 210, 256, 403, 508, 669, 693, 814, 1375
NlaIV GGNNCC 2 cut(s) 898, 1075
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 2 cut(s) 175, 766
NspI RCATGY 2 cut(s) 210, 508
OliI CACNNNNGTG 1 cut(s) 1178
PciI ACATGT 1 cut(s) 504
PciSI GCTCTTC 4 cut(s) 1018, 1032, 1195, 1209
PctI GAATGC 2 cut(s) 650, 874
PdmI GAANNNNTTC 2 cut(s) 1012, 1189
PfeI GAWTC 5 cut(s) 363, 529, 538, 1100, 1294
PkrI GCNGC 2 cut(s) 139, 486
PleI GAGTC 3 cut(s) 110, 250, 944
PmaCI CACGTG 1 cut(s) 418
PmlI CACGTG 1 cut(s) 418
PpsI GAGTC 3 cut(s) 110, 250, 944
Ppu21I YACGTR 1 cut(s) 418
PscI ACATGT 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 837
Psp6I CCWGG 1 cut(s) 659
PspCI CACGTG 1 cut(s) 418
PspEI GGTNACC 1 cut(s) 790
PspGI CCWGG 1 cut(s) 659
PspN4I GGNNCC 2 cut(s) 898, 1075
PsuI RGATCY 4 cut(s) 375, 381, 896, 1073
PvuII CAGCTG 1 cut(s) 646
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 1178
SacI GAGCTC 1 cut(s) 837
SapI GCTCTTC 4 cut(s) 1018, 1032, 1195, 1209
SaqAI TTAA 5 cut(s) 696, 723, 1058, 1235, 1358
SatI GCNGC 2 cut(s) 138, 485
Sau3AI GATC 4 cut(s) 375, 381, 896, 1073
SchI GAGTC 3 cut(s) 110, 250, 944
ScrFI CCNGG 3 cut(s) 661, 1230, 1247
SduI GDGCHC 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
SpeI ACTAGT 1 cut(s) 122
SsiI CCGC 1 cut(s) 138
SspMI CTAG 1 cut(s) 123
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 3 cut(s) 659, 1228, 1245
TaaI ACNGT 1 cut(s) 1328
TaiI ACGT 1 cut(s) 420
TaqI TCGA 7 cut(s) 56, 246, 336, 513, 541, 700, 887
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 5 cut(s) 363, 529, 538, 1100, 1294
Tru1I TTAA 5 cut(s) 696, 723, 1058, 1235, 1358
Tru9I TTAA 5 cut(s) 696, 723, 1058, 1235, 1358
TscAI CASTG 1 cut(s) 792
TseFI GTSAC 2 cut(s) 175, 766
TseI GCWGC 1 cut(s) 484
Tsp45I GTSAC 2 cut(s) 175, 766
TspDTI ATGAA 7 cut(s) 262, 388, 436, 596, 678, 984, 1161
TspRI CASTG 1 cut(s) 792
XapI RAATTY 4 cut(s) 674, 685, 922, 1391
XceI RCATGY 2 cut(s) 210, 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 803
XmnI GAANNNNTTC 2 cut(s) 1012, 1189
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.