Rh3DG266900

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0011578
Physical Location & Seq
Forward (+)
26656922 .. 26673405
16484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG266900.1

Sequence Viewer

Length: 1413 bp
ATGGCGGACCAGAGGAATGGTAGCCTTGAAGATACACCAACATGGGCTGTATCAGTATTTGCTTTGTTTATCTTCGTGCTATCCTTTATAATCGATGATGGTCTTCATCGTCTAACGAAGTTTCTTAAAGGAAGGAGAAGAAAATCCCTGAATAGGGCTTTGAAGAAGTTCAAAACAGAAATGATGCAACTGGGTTTCTTATCACTGCTTCTTACTCTGTCGGAAGTACCAATATCGAATATCTGTGTCTCTCAAGTTTTGGCAAACTCTTTTCTTCCCTGCGAGTATCCCTCGGACTCAACTAGTGTCTCATCAGCCGCACAACTTCCAGCTTCAAATACCAACTTCTCTAAGGAAGTCACCACTCAAACTTACTGCGAGGCAAAGGTTTGTTTTGCTCGGCAGTTTGGTGGCTCAATATCAAAAGTAGATTACATGACTCTGCGAAATGGGTTCATTGTGGCCAACATCAAAGAAGGTAGCAGCTTCAATTTCCTGAACTTCCTTGCTAGAGCTTTCGATGACGATTTTGAGCAAGTAGTTGGAATCAAATTACGTCAACCTTATTCTGGATTAATAACATCTGGTGTCATTTCTTTAATCAAGATTGTTCTACTCGTGGGAACAAAGCTGCAAGTCGTGATAACTAAAATGTGCGCGGAGAGTTGTAAGAAGAATCCTGTGATTCGAGGAAGCTTTGTGGTGGAGCTCAACAATGATTTATTCTGGTTTGGTCAACCCAATTGGCTTCTTCATCTCCTACAGTTTGTCCTAATCCAGATGGGGTCTGGCATGAGGAAAGCAGTATTTACTGAGCTCGTAGTTGATGGCCTAAAAAACTGGCACAAGAATGCAAGCCACAGACTATTGAAGAATGGATCCACTACTTCAAGGAAATCATCAAATTCTGCACATTTATATTCAACAGATGACTCCTCAATTCTAGACAATGAATGTAGCTACAAAACTCCTAATCTAACCATCGTGAGAAGCTCTTCTTCTACCTCCGAGATAATTGAAGAGCAGGTTCCACCCGGTTTAACTCCGAAGAATGGATCCAGTACTTCAAGAAAATTATCAAATTCTGCACAATTTTATTCAACTGATGACTTCTCAATTCCAGACAATGAATGTAGCTACAAAACTCCTCATCCCACCATTGTGAGAAGCTCTTCTTCTACCTCCGAAATAATTGAAGAGCACGTTTCACCCGGTTTAACTCCAAACCTGGATACTACAAGCTTACCAACTCAAGAAATCACCAGAGACGAAGAGAAACCCAACATCATCAACAAAATAATCTATGACGGTGAGATCTCATTTGGGAGCAGTTGGAAATTAAAGCTGGAGAGCATTAGCAAGAGAAGTGGAGAAATCACTTCAATAATTGAAGAAGAAGTTAACTTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

470

Amino Acids

52.25

Weight (kDa)

7.04

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 103 1.2e-27 Mlo family
Mlo PF03094 122 - 260 2.7e-35 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 1015
AccII CGCG 1 cut(s) 659
AciI CCGC 3 cut(s) 5, 318, 659
AclWI GGATC 4 cut(s) 873, 886, 1050, 1063
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 2 cut(s) 904, 1081
AfaI GTAC 2 cut(s) 228, 1063
AfiI CCNNNNNNNGG 4 cut(s) 153, 154, 569, 1052
AhlI ACTAGT 1 cut(s) 302
AjnI CCWGG 1 cut(s) 1227
AleI CACNNNNGTG 1 cut(s) 1160
Alw21I GWGCWC 3 cut(s) 711, 819, 1203
Alw26I GTCTC 3 cut(s) 253, 313, 1260
AlwI GGATC 4 cut(s) 873, 886, 1050, 1063
AoxI GGCC 2 cut(s) 462, 829
ApeKI GCWGC 2 cut(s) 483, 631
ApoI RAATTY 2 cut(s) 904, 1081
AseI ATTAAT 1 cut(s) 575
Asp700I GAANNNNTTC 3 cut(s) 167, 994, 1171
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 7
AsuC2I CCSGG 2 cut(s) 1035, 1212
AsuHPI GGTGA 4 cut(s) 352, 1200, 1252, 1322
AvaII GGWCC 1 cut(s) 7
BalI TGGCCA 1 cut(s) 464
BamHI GGATCC 2 cut(s) 878, 1055
BanII GRGCYC 2 cut(s) 711, 819
BauI CACGAG 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 3 cut(s) 711, 819, 1203
BbvI GCAGC 2 cut(s) 495, 618
BccI CCATC 4 cut(s) 92, 775, 821, 989
BciT130I CCWGG 1 cut(s) 1229
BciVI GTATCC 2 cut(s) 297, 1225
BcnI CCSGG 2 cut(s) 1035, 1212
BcoDI GTCTC 3 cut(s) 253, 313, 1260
BcuI ACTAGT 1 cut(s) 302
BfaI CTAG 3 cut(s) 303, 510, 944
BfmI CTRYAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 1015
BfuI GTATCC 2 cut(s) 297, 1225
BglII AGATCT 1 cut(s) 1314
BisI GCNGC 3 cut(s) 318, 484, 632
BlsI GCNGC 3 cut(s) 319, 485, 633
BmcAI AGTACT 1 cut(s) 1063
Bme1390I CCNGG 3 cut(s) 1035, 1212, 1229
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 3 cut(s) 880, 1029, 1057
BmrFI CCNGG 3 cut(s) 1035, 1212, 1229
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 174
BmuI ACTGGG 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 95
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpmI CTGGAG 1 cut(s) 1367
BpuEI CTTGAG 2 cut(s) 237, 1236
BpuMI CCSGG 2 cut(s) 1035, 1212
Bsa29I ATCGAT 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 291
Bsc4I CCNNNNNNNGG 4 cut(s) 153, 154, 569, 1052
Bse1I ACTGG 3 cut(s) 195, 845, 1059
BseBI CCWGG 1 cut(s) 1229
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 1 cut(s) 1150
BseLI CCNNNNNNNGG 4 cut(s) 153, 154, 569, 1052
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 3 cut(s) 195, 845, 1059
BseRI GAGGAG 2 cut(s) 925, 1137
BseXI GCAGC 2 cut(s) 495, 618
BsgI GTGCAG 2 cut(s) 894, 1071
Bsh1236I CGCG 1 cut(s) 659
BshFI GGCC 2 cut(s) 464, 831
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 3 cut(s) 711, 819, 1203
BsiSI CCGG 2 cut(s) 1035, 1212
BslI CCNNNNNNNGG 4 cut(s) 153, 154, 569, 1052
BsmAI GTCTC 3 cut(s) 253, 313, 1260
BsmBI CGTCTC 1 cut(s) 1260
BsmI GAATGC 1 cut(s) 856
BsnI GGCC 2 cut(s) 464, 831
Bsp1286I GDGCHC 3 cut(s) 711, 819, 1203
Bsp143I GATC 3 cut(s) 878, 1055, 1314
BspACI CCGC 3 cut(s) 5, 318, 659
BspANI GGCC 2 cut(s) 464, 831
BspCNI CTCAG 1 cut(s) 805
BspDI ATCGAT 1 cut(s) 93
BspFNI CGCG 1 cut(s) 659
BspLI GGNNCC 3 cut(s) 880, 1029, 1057
BspMI ACCTGC 1 cut(s) 1015
BspPI GGATC 4 cut(s) 873, 886, 1050, 1063
BspQI GCTCTTC 4 cut(s) 1000, 1014, 1177, 1191
BsrI ACTGG 3 cut(s) 195, 845, 1059
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 3 cut(s) 878, 1055, 1314
BssSI CACGAG 1 cut(s) 617
Bst2BI CACGAG 1 cut(s) 617
Bst2UI CCWGG 1 cut(s) 1229
Bst4CI ACNGT 2 cut(s) 765, 1310
Bst6I CTCTTC 5 cut(s) 1000, 1014, 1177, 1191, 1266
BstC8I GCNNGC 1 cut(s) 856
BstDEI CTNAG 2 cut(s) 351, 813
BstF5I GGATG 1 cut(s) 1150
BstFNI CGCG 1 cut(s) 659
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 3 cut(s) 881, 1058, 1317
BstMAI GTCTC 3 cut(s) 253, 313, 1260
BstMBI GATC 3 cut(s) 878, 1055, 1314
BstNI CCWGG 1 cut(s) 1229
BstSCI CCNGG 3 cut(s) 1033, 1210, 1227
BstSFI CTRYAG 1 cut(s) 761
BstUI CGCG 1 cut(s) 659
BstV1I GCAGC 2 cut(s) 495, 618
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 3 cut(s) 878, 1055, 1314
BstXI CCANNNNNNTGG 1 cut(s) 17
BstYI RGATCY 3 cut(s) 878, 1055, 1314
Bsu15I ATCGAT 1 cut(s) 93
BsuI GTATCC 2 cut(s) 297, 1225
BsuRI GGCC 2 cut(s) 464, 831
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 1 cut(s) 1150
BtsI GCAGTG 1 cut(s) 203
BtsIMutI CAGTG 1 cut(s) 203
BveI ACCTGC 1 cut(s) 1015
Cac8I GCNNGC 1 cut(s) 856
CfoI GCGC 1 cut(s) 659
Cfr13I GGNCC 1 cut(s) 7
ClaI ATCGAT 1 cut(s) 93
Csp6I GTAC 2 cut(s) 227, 1062
CspCI CAANNNNNGTGG 2 cut(s) 1348, 1383
CviAII CATG 3 cut(s) 42, 436, 793
CviQI GTAC 2 cut(s) 227, 1062
DdeI CTNAG 2 cut(s) 351, 813
DpnI GATC 3 cut(s) 880, 1057, 1316
DpnII GATC 3 cut(s) 878, 1055, 1314
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 5 cut(s) 1000, 1014, 1177, 1191, 1266
EarI CTCTTC 5 cut(s) 1000, 1014, 1177, 1191, 1266
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 709, 817
Eco24I GRGCYC 2 cut(s) 711, 819
Eco47I GGWCC 1 cut(s) 7
Eco53kI GAGCTC 2 cut(s) 709, 817
EcoICRI GAGCTC 2 cut(s) 709, 817
EcoRII CCWGG 1 cut(s) 1227
EcoT38I GRGCYC 2 cut(s) 711, 819
Esp3I CGTCTC 1 cut(s) 1260
FaeI CATG 3 cut(s) 45, 439, 796
FaiI YATR 6 cut(s) 43, 89, 437, 794, 919, 1305
FalI AAGNNNNNCTT 4 cut(s) 982, 1014, 1159, 1191
FatI CATG 3 cut(s) 41, 435, 792
Fnu4HI GCNGC 3 cut(s) 318, 484, 632
FokI GGATG 1 cut(s) 1137
FriOI GRGCYC 2 cut(s) 711, 819
Fsp4HI GCNGC 3 cut(s) 318, 484, 632
FspBI CTAG 3 cut(s) 303, 510, 944
GlaI GCGC 1 cut(s) 658
GluI GCNGC 3 cut(s) 318, 484, 632
GsuI CTGGAG 1 cut(s) 1367
HaeIII GGCC 2 cut(s) 464, 831
HapII CCGG 2 cut(s) 1035, 1212
HhaI GCGC 1 cut(s) 659
Hin1II CATG 3 cut(s) 45, 439, 796
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 3 cut(s) 560, 737, 1402
HindII GTYRAC 3 cut(s) 560, 737, 1402
HindIII AAGCTT 2 cut(s) 694, 1240
HinfI GANTC 6 cut(s) 296, 439, 546, 676, 685, 932
HpaI GTTAAC 1 cut(s) 1402
HpaII CCGG 2 cut(s) 1035, 1212
HphI GGTGA 4 cut(s) 352, 1200, 1252, 1322
Hpy166II GTNNAC 3 cut(s) 560, 737, 1402
Hpy188I TCNGA 5 cut(s) 223, 295, 1009, 1047, 1186
Hpy8I GTNNAC 3 cut(s) 560, 737, 1402
HpyAV CCTTC 2 cut(s) 126, 470
HpyCH4III ACNGT 2 cut(s) 765, 1310
HpyCH4IV ACGT 2 cut(s) 556, 1203
HpyCH4V TGCA 5 cut(s) 187, 634, 854, 911, 1088
HpyF3I CTNAG 2 cut(s) 351, 813
HpySE526I ACGT 2 cut(s) 556, 1203
Hsp92II CATG 3 cut(s) 45, 439, 796
HspAI GCGC 1 cut(s) 657
KspAI GTTAAC 1 cut(s) 1402
Kzo9I GATC 3 cut(s) 878, 1055, 1314
LguI GCTCTTC 4 cut(s) 1000, 1014, 1177, 1191
LmnI GCTCC 2 cut(s) 706, 1326
Lsp1109I GCAGC 2 cut(s) 495, 618
LweI GCATC 1 cut(s) 174
MaeI CTAG 3 cut(s) 303, 510, 944
MaeII ACGT 2 cut(s) 556, 1203
MaeIII GTNAC 1 cut(s) 358
MalI GATC 3 cut(s) 880, 1057, 1316
MboI GATC 3 cut(s) 878, 1055, 1314
MfeI CAATTG 1 cut(s) 742
MflI RGATCY 3 cut(s) 878, 1055, 1314
MhlI GDGCHC 3 cut(s) 711, 819, 1203
MlsI TGGCCA 1 cut(s) 464
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 3 cut(s) 290, 433, 926
MmeI TCCRAC 3 cut(s) 201, 523, 1313
MnlI CCTC 9 cut(s) 6, 301, 373, 683, 789, 946, 1015, 1158, 1192
Mox20I TGGCCA 1 cut(s) 464
MroXI GAANNNNTTC 3 cut(s) 167, 994, 1171
MscI TGGCCA 1 cut(s) 464
MseI TTAA 7 cut(s) 126, 575, 599, 1040, 1217, 1340, 1401
MslI CAYNNNNRTG 3 cut(s) 40, 849, 1160
Msp20I TGGCCA 1 cut(s) 464
MspI CCGG 2 cut(s) 1035, 1212
MspR9I CCNGG 3 cut(s) 1035, 1212, 1229
MunI CAATTG 1 cut(s) 742
Mva1269I GAATGC 1 cut(s) 856
MvaI CCWGG 1 cut(s) 1229
MvnI CGCG 1 cut(s) 659
NciI CCSGG 2 cut(s) 1035, 1212
NdeII GATC 3 cut(s) 878, 1055, 1314
NlaIII CATG 3 cut(s) 45, 439, 796
NlaIV GGNNCC 3 cut(s) 880, 1029, 1057
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 1160
PciSI GCTCTTC 4 cut(s) 1000, 1014, 1177, 1191
PctI GAATGC 1 cut(s) 856
PdmI GAANNNNTTC 3 cut(s) 167, 994, 1171
PfeI GAWTC 3 cut(s) 546, 676, 685
PkrI GCNGC 3 cut(s) 319, 485, 633
PleI GAGTC 3 cut(s) 290, 433, 926
PpsI GAGTC 3 cut(s) 290, 433, 926
PshBI ATTAAT 1 cut(s) 575
PsiI TTATAA 1 cut(s) 89
Psp124BI GAGCTC 2 cut(s) 711, 819
Psp6I CCWGG 1 cut(s) 1227
PspGI CCWGG 1 cut(s) 1227
PspN4I GGNNCC 3 cut(s) 880, 1029, 1057
PspPI GGNCC 1 cut(s) 7
PsuI RGATCY 3 cut(s) 878, 1055, 1314
RsaI GTAC 2 cut(s) 228, 1063
RsaNI GTAC 2 cut(s) 227, 1062
RseI CAYNNNNRTG 3 cut(s) 40, 849, 1160
SacI GAGCTC 2 cut(s) 711, 819
SapI GCTCTTC 4 cut(s) 1000, 1014, 1177, 1191
SaqAI TTAA 7 cut(s) 126, 575, 599, 1040, 1217, 1340, 1401
SatI GCNGC 3 cut(s) 318, 484, 632
Sau3AI GATC 3 cut(s) 878, 1055, 1314
Sau96I GGNCC 1 cut(s) 7
ScaI AGTACT 1 cut(s) 1063
SchI GAGTC 3 cut(s) 290, 433, 926
ScrFI CCNGG 3 cut(s) 1035, 1212, 1229
SduI GDGCHC 3 cut(s) 711, 819, 1203
SfaNI GCATC 1 cut(s) 174
SfcI CTRYAG 1 cut(s) 761
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 3 cut(s) 40, 849, 1160
SmlI CTYRAG 2 cut(s) 252, 1251
SmoI CTYRAG 2 cut(s) 252, 1251
SpeI ACTAGT 1 cut(s) 302
SsiI CCGC 3 cut(s) 5, 318, 659
SspMI CTAG 3 cut(s) 303, 510, 944
SstI GAGCTC 2 cut(s) 711, 819
StyD4I CCNGG 3 cut(s) 1033, 1210, 1227
TaaI ACNGT 2 cut(s) 765, 1310
TaiI ACGT 2 cut(s) 559, 1206
TaqI TCGA 5 cut(s) 93, 236, 519, 688, 1407
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 3 cut(s) 546, 676, 685
Tru1I TTAA 7 cut(s) 126, 575, 599, 1040, 1217, 1340, 1401
Tru9I TTAA 7 cut(s) 126, 575, 599, 1040, 1217, 1340, 1401
TscAI CASTG 1 cut(s) 210
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 483, 631
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 5 cut(s) 95, 445, 743, 966, 1143
TspRI CASTG 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 7
VspI ATTAAT 1 cut(s) 575
XapI RAATTY 2 cut(s) 904, 1081
XbaI TCTAGA 1 cut(s) 943
XcmI CCANNNNNNNNNTGG 1 cut(s) 785
XmnI GAANNNNTTC 3 cut(s) 167, 994, 1171
XspI CTAG 3 cut(s) 303, 510, 944
ZrmI AGTACT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.