Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0007980

Physical Location & Seq

Genomic Coordinates Forward (+)

17578756 .. 17586329

Gene Structure 7574 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g23530.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGGAGATGGCAACCACCACCTTCCACAGCTCCTCCTCCTCCTCCTTCTCTATCCCAAACCCTAACCCTAGAACCAACACTCTCTCCTCTTCTCTTCCACTCTCCAAGCAACTCTTCGGTTTCACCTTCCCTTCTTCCTCCACCGCCAAAACCTCCACTCTCAGACTCCGCTCCCGAAACCCTACCTCCTTCGCTCCTACGCCGATCAAATGCTCCGTCTCTGAAGCCACTGAAGCCGCCGCCCCCGTCGAGAAGAAGAAATTGAAGAGGAGAAGTGATATAAGGAACATAGCCATTGTGGCGCATGTTGATCATGGAAAGACAACTCTGGTTGATTCCATGTTGAGGCAAGCGAAGGTGTTTCGTGATAACCAAGTTGTGCAAGAGAGGATAATGGACTCGAATGACATAGAGCGTGAGAGGGGAATTACAATTTTGAGTAAAAACACATCTATTACGTATAAAGACTCGAAAATTAATATAATTGACACTCCTGGACATTCTGACTTTGGAGGGGAGGTGGAGCGTATTCTCAATATGGTGGAAGGGATTCTTCTAGTGGTGGATTCTGTTGAGGGCCCCATGCCACAGACAAGATTTGTTTTGAAGAAGGCTCTAGAGTTTGGGCATGCTGTTGTTGTTGTCGTCAATAAAATTGATAGGCCTTCCGCCCGTCCGGATTTTGTCATCAATTCCACTTTTGAACTCTTTATTGAACTCAATGCTACTGATGAACAGTGTGATTTCCAAGCAATATATGCTAGTGGGATTAAAGGAAAGGCAGGGCTATCAGCTGATAATCTGTCAGATGATCTTGGCCCCCTTTTTGAGGCCATAATGAGATGCATCCCCGGACCGGCTATTGAAGAAGGTCCACTGCAAATGCTTGCTACAAATATCGAGTATGATGAGCATAAAGGACGAATAGCTATTGGACGCTTGCATGCTGGGGCTCTTCGAAAAGGAATGGATGTGAGGGTATGTACCTCAGAAGATTCCTGTCGGTTTGCTAAAGTTAGTGAGCTTTTTGTGTACGAGAAGTTCATTAGGGTCCCAGTAGACAGTGTGGAGGCTGGTGATATATGTGCTGTTTGTGGAATTAATGATATTCAGATTGGCGAGACAATTGCTGATAAGACACATGGGAAGCCATTACCTGCCATTAGGGTGGAGGAACCAACAGTGAAAATGGCTTTCGCTATAAATACTTCACCATTTGTTGGGCGCGAGGGAAAGTATGTTACGAGTAGGAACTTGCGAGATCGGCTTTCTCGTGAGCTTGAGCGTAACTTGGCTATGAGAGTTGAAGATGGTGAAACAGCAGATACCTTCATTGTTAGTGGACGAGGCACTTTGCATATTACCATATTGATTGAGAACATGCGAAGAGAAGGATATGAGTTTATGGTGGGACCTCCCAAAGTAATTAACAAAAGGGTGAATGACAAGTTGCTGGAACCTTATGAGATTGCCACTGTAGAGGTTCCCGAAGAGCACATGGGTCCCGTTGTTGAGCTACTTGGAAAAAGGCGGGGACAAATGTTTGATATGCAGGGGATGGGGTCAGAAGGCACAAACATACTCAAATATAAGATTCCAACTCGTGGCCTTCTTGGTTTACGAAATGCAATTTTAACAGCTTCTCGTGGCACAGCTATTCTCAATACGATATTTGATAGCTATGGACCTTGGGCTGGTGATATGAGTACCCGGGATCTGGGTTCACTGGTTGCCTTTGAGGGTGGAACGAGTACTTCTTATGCCCTTGCTAGTTCACAGGATAGAGGACAGTTGTTTATTGGTCCTGGGGTAGACGTATATAAAGGTCAGATAGTTGGCATCCATCAGCGACCTGGGGACTTATCTCTTAACGTATGTAAAAAAAAGGCTGCAACAAACATCCGTTCCAACAAAGAACAATCAGTAATTCTTGATACTCCACTGGATTACAGTCTGGATGACTGCATTGAGTACATTCAAGAGGATGAACTGGTGGAGGTCACTCCCTCAAGCATCCGGATGTGCAAGAACCCCAAGTTCTCAAAGAAATCATCTAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0031647 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050821 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

687

Amino Acids

75.59

Weight (kDa)

6.64

Isoelectric Point (pI)

43.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	93 - 284	9.9e-53	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	294 - 377	2.5e-08	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	307 - 377	9.2e-12	Elongation factor Tu domain 2
EFG_C	PF00679	483 - 569	8.6e-21	Elongation factor G C-terminus
BipA_C	PF21018	574 - 677	1.9e-38	TypA/BipA C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13650 AT5G13650
fragaria_vesca	FvH4_6g23530 FvH4_6g23530 FvH4_6g23530 FvH4_6g23530
malus_domestica	MD07G1028800.v1.1 MD12G1072500.v1.1 MD14G1069900.v1.1
prunus_persica	Prupe.7G064400_v2.0.a1
pyrus_communis	pycom12g06100 pycom12g06110 pycom14g05790
rosa_chinensis	RchiOBHm_Chr3g0481531
rosa_laevigata	RLG00000023388
rosa_multiflora	Rmu_sc0010846.1_g000003 Rmu_sc0040423.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400150 Rroxscaffold_6G00400350
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243700 Rh3BG278800 Rh3DG270600
rosa_wichuraiana	Rw3G021770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1165
AccB7I	CCANNNNNTGG	2 cut(s)	1220, 1604
AccBSI	CCGCTC	1 cut(s)	171
AccI	GTMKAC	2 cut(s)	1059, 1812
AccII	CGCG	1 cut(s)	1227
AccIII	TCCGGA	2 cut(s)	676, 2016
AciI	CCGC	6 cut(s)	144, 169, 237, 240, 669, 1531
AclWI	GGATC	1 cut(s)	1722
AcuI	CTGAAG	2 cut(s)	243, 252
AfaI	GTAC	5 cut(s)	985, 1034, 1708, 1753, 1973
AfiI	CCNNNNNNNGG	7 cut(s)	345, 829, 856, 1220, 1604, 1694, 1717
AgsI	TTSAA	7 cut(s)	265, 607, 704, 716, 866, 1307, 1979
AjnI	CCWGG	3 cut(s)	493, 1804, 1852
AjuI	GAANNNNNNNTTGG	2 cut(s)	98, 130
AluBI	AGCT	9 cut(s)	30, 794, 929, 1024, 1279, 1516, 1640, 1655, 1680
AluI	AGCT	9 cut(s)	30, 794, 929, 1024, 1279, 1516, 1640, 1655, 1680
Alw21I	GWGCWC	1 cut(s)	1497
Alw26I	GTCTC	2 cut(s)	223, 1115
AlwI	GGATC	1 cut(s)	1722
Ama87I	CYCGRG	1 cut(s)	1710
Aor13HI	TCCGGA	2 cut(s)	676, 2016
AoxI	GGCC	5 cut(s)	577, 662, 817, 831, 1606
ApaI	GGGCCC	1 cut(s)	581
ApeKI	GCWGC	1 cut(s)	1889
ArsI	GACNNNNNNTTYG	2 cut(s)	201, 233
AseI	ATTAAT	2 cut(s)	477, 1101
Asp700I	GAANNNNTTC	1 cut(s)	549
AspLEI	GCGC	2 cut(s)	304, 1227
AsuC2I	CCSGG	3 cut(s)	852, 1711, 1712
AsuHPI	GGTGA	6 cut(s)	115, 1088, 1203, 1325, 1450, 1709
AsuII	TTCGAA	1 cut(s)	958
AvaI	CYCGRG	1 cut(s)	1710
AvaII	GGWCC	7 cut(s)	854, 872, 1051, 1412, 1502, 1685, 1802
BaeGI	GKGCMC	1 cut(s)	581
BanII	GRGCYC	2 cut(s)	581, 955
BauI	CACGAG	3 cut(s)	1272, 1602, 1644
Bbv12I	GWGCWC	1 cut(s)	1497
BbvI	GCAGC	1 cut(s)	1876
BccI	CCATC	3 cut(s)	1304, 1552, 1851
BcgI	CGANNNNNNTGC	2 cut(s)	1109, 1143
BciT130I	CCWGG	3 cut(s)	495, 1806, 1854
BclI	TGATCA	1 cut(s)	310
BcnI	CCSGG	3 cut(s)	852, 1711, 1712
BcoDI	GTCTC	2 cut(s)	223, 1115
BfaI	CTAG	6 cut(s)	69, 557, 617, 762, 1770, 2055
BfmI	CTRYAG	1 cut(s)	1476
BfuAI	ACCTGC	1 cut(s)	1165
BglI	GCCNNNNNGGC	1 cut(s)	299
BisI	GCNGC	3 cut(s)	237, 240, 1890
BlsI	GCNGC	3 cut(s)	238, 241, 1891
BmcAI	AGTACT	1 cut(s)	1753
Bme1390I	CCNGG	6 cut(s)	495, 852, 1711, 1712, 1806, 1854
Bme18I	GGWCC	7 cut(s)	854, 872, 1051, 1412, 1502, 1685, 1802
BmeT110I	CYCGRG	1 cut(s)	1710
BmrFI	CCNGG	6 cut(s)	495, 852, 1711, 1712, 1806, 1854
BmrI	ACTGGG	1 cut(s)	1049
BmsI	GCATC	4 cut(s)	833, 855, 1848, 2022
BmuI	ACTGGG	1 cut(s)	1049
Bpu14I	TTCGAA	1 cut(s)	958
BpuEI	CTTGAG	2 cut(s)	1301, 1993
BpuMI	CCSGG	3 cut(s)	852, 1711, 1712
BsaAI	YACGTR	1 cut(s)	459
BsaJI	CCNNGG	5 cut(s)	850, 1688, 1710, 1805, 1853
BsaWI	WCCGGW	2 cut(s)	676, 2016
BsaXI	ACNNNNNCTCC	8 cut(s)	17, 47, 68, 98, 170, 200, 504, 534
Bsc4I	CCNNNNNNNGG	7 cut(s)	345, 829, 856, 1220, 1604, 1694, 1717
Bse118I	RCCGGY	1 cut(s)	856
Bse1I	ACTGG	4 cut(s)	1055, 1731, 1947, 1995
BseAI	TCCGGA	2 cut(s)	676, 2016
BseBI	CCWGG	3 cut(s)	495, 1806, 1854
BseDI	CCNNGG	5 cut(s)	850, 1688, 1710, 1805, 1853
BseGI	GGATG	9 cut(s)	846, 976, 1563, 1839, 1899, 1963, 1990, 2013, 2025
BseLI	CCNNNNNNNGG	7 cut(s)	345, 829, 856, 1220, 1604, 1694, 1717
BseMII	CTCAG	2 cut(s)	175, 1002
BseNI	ACTGG	4 cut(s)	1055, 1731, 1947, 1995
BseRI	GAGGAG	6 cut(s)	22, 25, 28, 31, 76, 283
BseSI	GKGCMC	1 cut(s)	581
BseXI	GCAGC	1 cut(s)	1876
BseYI	CCCAGC	1 cut(s)	947
Bsh1236I	CGCG	1 cut(s)	1227
BshFI	GGCC	5 cut(s)	579, 664, 819, 833, 1608
BsiHKAI	GWGCWC	1 cut(s)	1497
BsiHKCI	CYCGRG	1 cut(s)	1710
BsiSI	CCGG	5 cut(s)	677, 852, 857, 1711, 2017
BslFI	GGGAC	5 cut(s)	1037, 1425, 1488, 1548, 1871
BslI	CCNNNNNNNGG	7 cut(s)	345, 829, 856, 1220, 1604, 1694, 1717
BsmAI	GTCTC	2 cut(s)	223, 1115
BsmBI	CGTCTC	1 cut(s)	223
BsmFI	GGGAC	5 cut(s)	1037, 1425, 1488, 1548, 1871
BsnI	GGCC	5 cut(s)	579, 664, 819, 833, 1608
BsoBI	CYCGRG	1 cut(s)	1710
Bsp119I	TTCGAA	1 cut(s)	958
Bsp120I	GGGCCC	1 cut(s)	577
Bsp1286I	GDGCHC	3 cut(s)	581, 955, 1497
Bsp13I	TCCGGA	2 cut(s)	676, 2016
Bsp143I	GATC	5 cut(s)	204, 310, 811, 1261, 1714
BspACI	CCGC	6 cut(s)	144, 169, 237, 240, 669, 1531
BspANI	GGCC	5 cut(s)	579, 664, 819, 833, 1608
BspCNI	CTCAG	2 cut(s)	174, 1001
BspEI	TCCGGA	2 cut(s)	676, 2016
BspFNI	CGCG	1 cut(s)	1227
BspMI	ACCTGC	1 cut(s)	1165
BspPI	GGATC	1 cut(s)	1722
BspQI	GCTCTTC	2 cut(s)	960, 1485
BspT104I	TTCGAA	1 cut(s)	958
BsrBI	CCGCTC	1 cut(s)	171
BsrFI	RCCGGY	1 cut(s)	856
BsrI	ACTGG	4 cut(s)	1055, 1731, 1947, 1995
BssAI	RCCGGY	1 cut(s)	856
BssECI	CCNNGG	5 cut(s)	850, 1688, 1710, 1805, 1853
BssMI	GATC	5 cut(s)	204, 310, 811, 1261, 1714
BssSI	CACGAG	3 cut(s)	1272, 1602, 1644
BssT1I	CCWWGG	1 cut(s)	1688
Bst2BI	CACGAG	3 cut(s)	1272, 1602, 1644
Bst2UI	CCWGG	3 cut(s)	495, 1806, 1854
Bst4CI	ACNGT	6 cut(s)	738, 1064, 1183, 1477, 1791, 1952
Bst6I	CTCTTC	7 cut(s)	94, 99, 119, 260, 960, 1381, 1485
BstAPI	GCANNNNNTGC	1 cut(s)	758
BstBAI	YACGTR	1 cut(s)	459
BstBI	TTCGAA	1 cut(s)	958
BstC8I	GCNNGC	5 cut(s)	351, 630, 888, 941, 945
BstDEI	CTNAG	2 cut(s)	161, 988
BstENI	CCTNNNNNAGG	1 cut(s)	827
BstF5I	GGATG	9 cut(s)	846, 976, 1563, 1839, 1899, 1963, 1990, 2013, 2025
BstFNI	CGCG	1 cut(s)	1227
BstHHI	GCGC	2 cut(s)	304, 1227
BstKTI	GATC	5 cut(s)	207, 313, 814, 1264, 1717
BstMAI	GTCTC	2 cut(s)	223, 1115
BstMBI	GATC	5 cut(s)	204, 310, 811, 1261, 1714
BstMWI	GCNNNNNNNGC	4 cut(s)	233, 299, 758, 1264
BstNI	CCWGG	3 cut(s)	495, 1806, 1854
BstNSI	RCATGY	4 cut(s)	308, 632, 947, 1384
BstSCI	CCNGG	6 cut(s)	493, 850, 1709, 1710, 1804, 1852
BstSFI	CTRYAG	1 cut(s)	1476
BstSLI	GKGCMC	1 cut(s)	581
BstSNI	TACGTA	1 cut(s)	459
BstUI	CGCG	1 cut(s)	1227
BstV1I	GCAGC	1 cut(s)	1876
BstX2I	RGATCY	1 cut(s)	1714
BstXI	CCANNNNNNTGG	1 cut(s)	1168
BstYI	RGATCY	1 cut(s)	1714
BsuRI	GGCC	5 cut(s)	579, 664, 819, 833, 1608
BtsCI	GGATG	9 cut(s)	846, 976, 1563, 1839, 1899, 1963, 1990, 2013, 2025
BtsI	GCAGTG	1 cut(s)	875
BtsIMutI	CAGTG	8 cut(s)	228, 743, 875, 1069, 1188, 1473, 1724, 1940
BveI	ACCTGC	1 cut(s)	1165
Cac8I	GCNNGC	5 cut(s)	351, 630, 888, 941, 945
CfoI	GCGC	2 cut(s)	304, 1227
Cfr10I	RCCGGY	1 cut(s)	856
Cfr9I	CCCGGG	1 cut(s)	1710
CpoI	CGGWCCG	1 cut(s)	854
CseI	GACGC	1 cut(s)	945
Csp6I	GTAC	5 cut(s)	984, 1033, 1707, 1752, 1972
CspI	CGGWCCG	1 cut(s)	854
CviAII	CATG	9 cut(s)	305, 314, 340, 583, 629, 944, 1142, 1381, 1498
CviQI	GTAC	5 cut(s)	984, 1033, 1707, 1752, 1972
DdeI	CTNAG	2 cut(s)	161, 988
DpnI	GATC	5 cut(s)	206, 312, 813, 1263, 1716
DpnII	GATC	5 cut(s)	204, 310, 811, 1261, 1714
Eam1104I	CTCTTC	7 cut(s)	94, 99, 119, 260, 960, 1381, 1485
EarI	CTCTTC	7 cut(s)	94, 99, 119, 260, 960, 1381, 1485
EciI	GGCGGA	1 cut(s)	658
Eco105I	TACGTA	1 cut(s)	459
Eco130I	CCWWGG	1 cut(s)	1688
Eco147I	AGGCCT	1 cut(s)	664
Eco24I	GRGCYC	2 cut(s)	581, 955
Eco47I	GGWCC	7 cut(s)	854, 872, 1051, 1412, 1502, 1685, 1802
Eco57I	CTGAAG	2 cut(s)	243, 252
Eco88I	CYCGRG	1 cut(s)	1710
EcoNI	CCTNNNNNAGG	1 cut(s)	827
EcoO109I	RGGNCCY	5 cut(s)	577, 578, 1051, 1412, 1502
EcoRII	CCWGG	3 cut(s)	493, 1804, 1852
EcoT14I	CCWWGG	1 cut(s)	1688
EcoT22I	ATGCAT	1 cut(s)	848
EcoT38I	GRGCYC	2 cut(s)	581, 955
ErhI	CCWWGG	1 cut(s)	1688
Esp3I	CGTCTC	1 cut(s)	223
FaeI	CATG	9 cut(s)	308, 317, 343, 586, 632, 947, 1145, 1384, 1501
FalI	AAGNNNNNCTT	4 cut(s)	98, 130, 537, 569
FaqI	GGGAC	5 cut(s)	1037, 1425, 1488, 1548, 1871
FatI	CATG	9 cut(s)	304, 313, 339, 582, 628, 943, 1141, 1380, 1497
FauI	CCCGC	1 cut(s)	1524
FbaI	TGATCA	1 cut(s)	310
FblI	GTMKAC	2 cut(s)	1059, 1812
Fnu4HI	GCNGC	3 cut(s)	237, 240, 1890
FokI	GGATG	9 cut(s)	833, 983, 1570, 1826, 1886, 1970, 1997, 2000, 2032
FriOI	GRGCYC	2 cut(s)	581, 955
Fsp4HI	GCNGC	3 cut(s)	237, 240, 1890
FspBI	CTAG	6 cut(s)	69, 557, 617, 762, 1770, 2055
GlaI	GCGC	2 cut(s)	303, 1226
GluI	GCNGC	3 cut(s)	237, 240, 1890
GsaI	CCCAGC	1 cut(s)	951
HaeIII	GGCC	5 cut(s)	579, 664, 819, 833, 1608
HapII	CCGG	5 cut(s)	677, 852, 857, 1711, 2017
HgaI	GACGC	1 cut(s)	945
HhaI	GCGC	2 cut(s)	304, 1227
Hin1II	CATG	9 cut(s)	308, 317, 343, 586, 632, 947, 1145, 1384, 1501
Hin6I	GCGC	2 cut(s)	302, 1225
HinP1I	GCGC	2 cut(s)	302, 1225
HinfI	GANTC	8 cut(s)	165, 335, 398, 467, 550, 566, 995, 1594
HpaII	CCGG	5 cut(s)	677, 852, 857, 1711, 2017
HphI	GGTGA	6 cut(s)	115, 1088, 1203, 1325, 1450, 1709
Hpy166II	GTNNAC	8 cut(s)	875, 1033, 1060, 1343, 1619, 1724, 1775, 1813
Hpy188I	TCNGA	8 cut(s)	164, 223, 505, 808, 991, 1113, 1567, 1830
Hpy8I	GTNNAC	8 cut(s)	875, 1033, 1060, 1343, 1619, 1724, 1775, 1813
Hpy99I	CGWCG	1 cut(s)	251
HpyCH4III	ACNGT	6 cut(s)	738, 1064, 1183, 1477, 1791, 1952
HpyCH4IV	ACGT	3 cut(s)	458, 1815, 1872
HpyF10VI	GCNNNNNNNGC	4 cut(s)	233, 299, 758, 1264
HpyF3I	CTNAG	2 cut(s)	161, 988
HpySE526I	ACGT	3 cut(s)	458, 1815, 1872
Hsp92II	CATG	9 cut(s)	308, 317, 343, 586, 632, 947, 1145, 1384, 1501
HspAI	GCGC	2 cut(s)	302, 1225
KflI	GGGWCCC	2 cut(s)	1051, 1502
Kpn2I	TCCGGA	2 cut(s)	676, 2016
Ksp22I	TGATCA	1 cut(s)	310
Kzo9I	GATC	5 cut(s)	204, 310, 811, 1261, 1714
LguI	GCTCTTC	2 cut(s)	960, 1485
LmnI	GCTCC	5 cut(s)	35, 176, 199, 218, 523
Lsp1109I	GCAGC	1 cut(s)	1876
LweI	GCATC	4 cut(s)	833, 855, 1848, 2022
MaeI	CTAG	6 cut(s)	69, 557, 617, 762, 1770, 2055
MaeII	ACGT	3 cut(s)	458, 1815, 1872
MaeIII	GTNAC	3 cut(s)	1240, 1286, 1999
MalI	GATC	5 cut(s)	206, 312, 813, 1263, 1716
MbiI	CCGCTC	1 cut(s)	171
MboI	GATC	5 cut(s)	204, 310, 811, 1261, 1714
MfeI	CAATTG	1 cut(s)	1125
MflI	RGATCY	1 cut(s)	1714
MhlI	GDGCHC	3 cut(s)	581, 955, 1497
MlyI	GAGTC	3 cut(s)	159, 392, 461
MmeI	TCCRAC	2 cut(s)	1622, 1932
Mph1103I	ATGCAT	1 cut(s)	848
MroI	TCCGGA	2 cut(s)	676, 2016
MroXI	GAANNNNTTC	1 cut(s)	549
MseI	TTAA	6 cut(s)	477, 771, 1101, 1428, 1634, 1869
MslI	CAYNNNNRTG	2 cut(s)	1166, 2018
MspA1I	CMGCKG	1 cut(s)	794
MspI	CCGG	5 cut(s)	677, 852, 857, 1711, 2017
MspR9I	CCNGG	6 cut(s)	495, 852, 1711, 1712, 1806, 1854
MunI	CAATTG	1 cut(s)	1125
MvaI	CCWGG	3 cut(s)	495, 1806, 1854
MvnI	CGCG	1 cut(s)	1227
MwoI	GCNNNNNNNGC	4 cut(s)	233, 299, 758, 1264
NciI	CCSGG	3 cut(s)	852, 1711, 1712
NdeII	GATC	5 cut(s)	204, 310, 811, 1261, 1714
NlaIII	CATG	9 cut(s)	308, 317, 343, 586, 632, 947, 1145, 1384, 1501
NmuCI	GTSAC	1 cut(s)	1999
NsiI	ATGCAT	1 cut(s)	848
NspI	RCATGY	4 cut(s)	308, 632, 947, 1384
NspV	TTCGAA	1 cut(s)	958
PaeI	GCATGC	2 cut(s)	632, 947
PceI	AGGCCT	1 cut(s)	664
PciSI	GCTCTTC	2 cut(s)	960, 1485
PcsI	WCGNNNNNNNCGW	1 cut(s)	1270
PdmI	GAANNNNTTC	1 cut(s)	549
PfeI	GAWTC	5 cut(s)	335, 550, 566, 995, 1594
PflMI	CCANNNNNTGG	2 cut(s)	1220, 1604
PfoI	TCCNGGA	1 cut(s)	493
PkrI	GCNGC	3 cut(s)	238, 241, 1891
PleI	GAGTC	3 cut(s)	159, 392, 461
PpsI	GAGTC	3 cut(s)	159, 392, 461
Ppu21I	YACGTR	1 cut(s)	459
PpuMI	RGGWCCY	3 cut(s)	1051, 1412, 1502
PshBI	ATTAAT	2 cut(s)	477, 1101
Psp5II	RGGWCCY	3 cut(s)	1051, 1412, 1502
Psp6I	CCWGG	3 cut(s)	493, 1804, 1852
PspFI	CCCAGC	1 cut(s)	947
PspGI	CCWGG	3 cut(s)	493, 1804, 1852
PspOMI	GGGCCC	1 cut(s)	577
PspPPI	RGGWCCY	3 cut(s)	1051, 1412, 1502
PsrI	GAACNNNNNNTAC	2 cut(s)	1025, 1057
PsuI	RGATCY	1 cut(s)	1714
PvuII	CAGCTG	1 cut(s)	794
RsaI	GTAC	5 cut(s)	985, 1034, 1708, 1753, 1973
RsaNI	GTAC	5 cut(s)	984, 1033, 1707, 1752, 1972
RseI	CAYNNNNRTG	2 cut(s)	1166, 2018
Rsr2I	CGGWCCG	1 cut(s)	854
RsrII	CGGWCCG	1 cut(s)	854
SapI	GCTCTTC	2 cut(s)	960, 1485
SaqAI	TTAA	6 cut(s)	477, 771, 1101, 1428, 1634, 1869
SatI	GCNGC	3 cut(s)	237, 240, 1890
Sau3AI	GATC	5 cut(s)	204, 310, 811, 1261, 1714
ScaI	AGTACT	1 cut(s)	1753
SchI	GAGTC	3 cut(s)	159, 392, 461
ScrFI	CCNGG	6 cut(s)	495, 852, 1711, 1712, 1806, 1854
SduI	GDGCHC	3 cut(s)	581, 955, 1497
SfaNI	GCATC	4 cut(s)	833, 855, 1848, 2022
SfcI	CTRYAG	1 cut(s)	1476
SfuI	TTCGAA	1 cut(s)	958
SinI	GGWCC	7 cut(s)	854, 872, 1051, 1412, 1502, 1685, 1802
SmaI	CCCGGG	1 cut(s)	1712
SmiMI	CAYNNNNRTG	2 cut(s)	1166, 2018
SmlI	CTYRAG	2 cut(s)	1280, 2008
SmoI	CTYRAG	2 cut(s)	1280, 2008
SnaBI	TACGTA	1 cut(s)	459
SphI	GCATGC	2 cut(s)	632, 947
SseBI	AGGCCT	1 cut(s)	664
SsiI	CCGC	6 cut(s)	144, 169, 237, 240, 669, 1531
SspMI	CTAG	6 cut(s)	69, 557, 617, 762, 1770, 2055
StuI	AGGCCT	1 cut(s)	664
StyD4I	CCNGG	6 cut(s)	493, 850, 1709, 1710, 1804, 1852
StyI	CCWWGG	1 cut(s)	1688
TaaI	ACNGT	6 cut(s)	738, 1064, 1183, 1477, 1791, 1952
TaiI	ACGT	3 cut(s)	461, 1818, 1875
TaqI	TCGA	5 cut(s)	249, 401, 470, 900, 958
TatI	WGTACW	2 cut(s)	1751, 1971
TauI	GCSGC	2 cut(s)	239, 242
TfiI	GAWTC	5 cut(s)	335, 550, 566, 995, 1594
Tru1I	TTAA	6 cut(s)	477, 771, 1101, 1428, 1634, 1869
Tru9I	TTAA	6 cut(s)	477, 771, 1101, 1428, 1634, 1869
TscAI	CASTG	8 cut(s)	235, 743, 882, 1069, 1188, 1480, 1731, 1947
TseFI	GTSAC	1 cut(s)	1999
TseI	GCWGC	1 cut(s)	1889
Tsp45I	GTSAC	1 cut(s)	1999
TspDTI	ATGAA	4 cut(s)	747, 1033, 1321, 2001
TspGWI	ACGGA	2 cut(s)	205, 1892
TspMI	CCCGGG	1 cut(s)	1710
TspRI	CASTG	8 cut(s)	235, 743, 882, 1069, 1188, 1480, 1731, 1947
Van91I	CCANNNNNTGG	2 cut(s)	1220, 1604
VpaK11BI	GGWCC	7 cut(s)	854, 872, 1051, 1412, 1502, 1685, 1802
VspI	ATTAAT	2 cut(s)	477, 1101
XagI	CCTNNNNNAGG	1 cut(s)	827
XbaI	TCTAGA	1 cut(s)	616
XceI	RCATGY	4 cut(s)	308, 632, 947, 1384
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1850
XmaI	CCCGGG	1 cut(s)	1710
XmiI	GTMKAC	2 cut(s)	1059, 1812
XmnI	GAANNNNTTC	1 cut(s)	549
XspI	CTAG	6 cut(s)	69, 557, 617, 762, 1770, 2055
ZrmI	AGTACT	1 cut(s)	1753
Zsp2I	ATGCAT	1 cut(s)	848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.