Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007980

Physical Location & Seq

Genomic Coordinates Forward (+)

8309886 .. 8318058

Gene Structure 8173 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1072500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2148 bp

ATGTTGCTTTGGAAGTTCCTCACTCGCCAAATCTCCTCTTTGTCTCTCTCCCTGAGGCTGAGCCGCTGCGAGCACAGAGTCAGAGCCATGGAGATGGCAACCTGCAGCTTCCACGGCTCCTCCTTCTCCATCCCAAACCCTAGAAGCACCAGCCTTTCCTCTCCTCTTCCAGTCGCCAAGCAACTCTTCTGCTTCACCTTACCTTCTTCCTCAACCGCCAAAACCACCACATTCAAACTCCGCTCCCGGAACCCGAAACGCTTCTCCGCTCACCCGCCAATCAAATGCTCCGTCTCTGAAGCCACCGAAGCCGCCGCCATTGAAACAGAGAGGAGGAGTAAGTTGATGAGGAGAACTGACATAAGGAACATAGCAATCGTAGCGCACGTCGACCACGGAAAAACAACTTTGGTTGATGCAATGTTGAGGCAATCAAAGGAAAGGATAATGGACTCAAATGATCTAGAGCGTGAAAGGGGAATTACTATACTAAGCAAAAATACCTCTATTACTTATAAAGACTCGAAGATTAATATAATTGATACTCCTGGACATTCTGACTTTGGAGGCGAAGTAGAACGCATCCTCAATATGGTGGAAGGGATTCTTTTAGTGGTAGATTCTGTTGAGGGCCCGATGCCACAGACAAGATTTGTTTTGAAGAAGGCTCTAGAATTTGGTCATGCAGTTGTTGTCGTTGTTAATAAGATTGACAGGCCTTCAGCTCGTCCAGATTTTGTCATCAATTCCACTTTTGAACTATTCATTGAACTAAATGCGACTGATGAACAGTGTGATTTCCAGGCAATATATGCTAGTGGGATTAAAGGAAAAGCGGGGTTGTCAGCTGATAATTTGGCAGATGATCTTGCACCACTTTTCGAGGCCATAATGAGATGCATCCCTGGACCTGCTATTGACAAAGATGGTGCACTTCAAATGCTTGTTACAAACATTGAGTATGATGAACATAAAGGGCGAATAGCTATTGGACGTTTGCATGCTGGGGTTCTGAGGAAAGGGATGGATGTGAAGGTATGCACCTCAGAAGATGCATGTCGAGTTGCAAGAGTTAGTGAGCTTTTTGTGTATGAAAAATTTAGTAGGGTTCCTGCAGAAAGTGTGGAGGCTGGTGATATATGTGCTGTTTGTGGAATCAATGATATTCAGATTGGCGAGACAATTGCTTGTAAGATCTCTGGTACACCATTACCAGCCATTAGGGTTGAGGAACCAACAGTGAAAATGGCTTTTGCCATAAACACTTCACCGTTTGTTGGACGTGAGGGAAAGTATGTTACCAGTAGGAACTTACGAGATCGCCTTTCTCGTGAGCTTGAGCGTAACTTGGCTATGAAGGTTGAAGATGGCGAAACAGCAGATACCTTCATTGTTAGTGGACGGGGTACTTTACATATTACCATATTGATCGAGAACATGCGAAGAGAAGGATATGAATTCATGGTGGGACCTCCCAAAGTTATTAACAAAAGGGTGAATGACAAATTGCTGGAACCATATGAGATCGCCACTGTGGAGGTGCCAGAAGAACACATGGGCCCTGTTGTTGAGCTTCTTGGCAAAAGGCGGGGGCAGATGTTTGATATGCAAGGAGTTGGGTGTCGTACCCAGTGCACAAGGCTCCTGCTTTACGCAGGCACAACCTTCCTCAAATATAAAATTCCAACTCGTGGCCTTCTTGGTTTACGAAATGCAATTTTAACAGCTTCTCGTGGCACTGCGATTCTCAACACAGTATTTGATAGCTATGGACCTTGGGCTGGTGATATGAGTACCCGGGATCAGGGTTCACTGGTTGCCTTTGAGGGTGGAACGAGTACTTCTTACGCCATTTCTAGCTCACAGGAGAGGGGGCAGTTGTTTATTGGTCCCGGGGTGGAAGTTTATAGAGGTCAGATAGTTGGCATCCATCAGCGGCCTGGGGACTTGTCCCTTAATGTGTGTAAGAAGAAGGCTGCAACAAATATCCGTTCCAACAAAGAACAAACAGTGATTCTTGACACCCCATTGGACTACAGTCTGGATGACTGCATTGAGTATATTCAAGAGGATGAATTGGTGGAGGTCACTCCTGCAAGTATCCGAATGTGCAAGAACCCAAAGTTTTCAAAGAAATCAAACAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0031647 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050821 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

716

Amino Acids

79.18

Weight (kDa)

8.55

Isoelectric Point (pI)

42.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	120 - 302	1.5e-49	Elongation factor Tu GTP binding domain
MMR_HSR1	PF01926	124 - 236	1.3e-07	50S ribosome-binding GTPase
EF-G_D2	PF22042	313 - 395	1.2e-08	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	326 - 396	5.3e-12	Elongation factor Tu domain 2
EFG_C	PF00679	502 - 598	2.1e-16	Elongation factor G C-terminus
BipA_C	PF21018	602 - 706	1.3e-38	TypA/BipA C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13650 AT5G13650
fragaria_vesca	FvH4_6g23530 FvH4_6g23530 FvH4_6g23530 FvH4_6g23530
malus_domestica	MD07G1028800.v1.1 MD12G1072500.v1.1 MD14G1069900.v1.1
prunus_persica	Prupe.7G064400_v2.0.a1
pyrus_communis	pycom12g06100 pycom12g06110 pycom14g05790
rosa_chinensis	RchiOBHm_Chr3g0481531
rosa_laevigata	RLG00000023388
rosa_multiflora	Rmu_sc0010846.1_g000003 Rmu_sc0040423.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400150 Rroxscaffold_6G00400350
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243700 Rh3BG278800 Rh3DG270600
rosa_wichuraiana	Rw3G021770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	516
Acc36I	ACCTGC	2 cut(s)	110, 919
AccB1I	GGYRCC	1 cut(s)	1540
AccB7I	CCANNNNNTGG	1 cut(s)	1691
AccBSI	CCGCTC	2 cut(s)	243, 269
AccI	GTMKAC	1 cut(s)	390
AclWI	GGATC	1 cut(s)	1809
AcsI	RAATTY	4 cut(s)	674, 1097, 1457, 1680
AcuI	CTGAAG	2 cut(s)	318, 705
AfaI	GTAC	5 cut(s)	1204, 1408, 1627, 1795, 1840
AfiI	CCNNNNNNNGG	5 cut(s)	592, 1277, 1691, 1781, 1804
AgsI	TTSAA	9 cut(s)	235, 323, 661, 758, 770, 938, 1364, 2066, 2130
AjiI	CACGTC	2 cut(s)	388, 1283
AjnI	CCWGG	4 cut(s)	547, 801, 904, 1939
AjuI	GAANNNNNNNTTGG	2 cut(s)	170, 202
Alw21I	GWGCWC	3 cut(s)	75, 934, 1637
Alw26I	GTCTC	3 cut(s)	48, 298, 1172
Alw44I	GTGCAC	2 cut(s)	930, 1633
AlwI	GGATC	1 cut(s)	1809
Ama87I	CYCGRG	2 cut(s)	1797, 1892
AoxI	GGCC	6 cut(s)	631, 716, 885, 1558, 1693, 1937
ApaI	GGGCCC	2 cut(s)	635, 1562
ApaLI	GTGCAC	2 cut(s)	930, 1633
ApeKI	GCWGC	3 cut(s)	66, 105, 1976
ApoI	RAATTY	4 cut(s)	674, 1097, 1457, 1680
ArsI	GACNNNNNNTTYG	2 cut(s)	276, 308
AseI	ATTAAT	1 cut(s)	531
Asp700I	GAANNNNTTC	2 cut(s)	260, 603
AspLEI	GCGC	1 cut(s)	385
AspS9I	GGNCC	8 cut(s)	631, 632, 908, 1469, 1558, 1559, 1772, 1889
AsuC2I	CCSGG	5 cut(s)	247, 1798, 1799, 1893, 1894
AsuHPI	GGTGA	6 cut(s)	187, 263, 1145, 1260, 1507, 1796
AvaI	CYCGRG	2 cut(s)	1797, 1892
AvaII	GGWCC	4 cut(s)	908, 1469, 1772, 1889
AxyI	CCTNAGG	1 cut(s)	53
BaeGI	GKGCMC	4 cut(s)	635, 934, 1562, 1637
BanI	GGYRCC	1 cut(s)	1540
BanII	GRGCYC	2 cut(s)	635, 1562
BauI	CACGAG	3 cut(s)	1329, 1689, 1731
Bbv12I	GWGCWC	3 cut(s)	75, 934, 1637
BbvI	GCAGC	3 cut(s)	53, 117, 1963
BccI	CCATC	6 cut(s)	88, 137, 920, 1018, 1361, 1938
BceAI	ACGGC	1 cut(s)	130
BcgI	CGANNNNNNTGC	2 cut(s)	1166, 1200
BciT130I	CCWGG	4 cut(s)	549, 803, 906, 1941
BciVI	GTATCC	1 cut(s)	2111
BcnI	CCSGG	5 cut(s)	247, 1798, 1799, 1893, 1894
BcoDI	GTCTC	3 cut(s)	48, 298, 1172
BfaI	CTAG	5 cut(s)	141, 464, 671, 816, 1857
BfmI	CTRYAG	3 cut(s)	103, 1113, 2035
BfuAI	ACCTGC	2 cut(s)	110, 919
BfuI	GTATCC	1 cut(s)	2111
BglII	AGATCT	1 cut(s)	1194
BisI	GCNGC	7 cut(s)	64, 67, 106, 312, 315, 1937, 1977
BlpI	GCTNAGC	1 cut(s)	59
BlsI	GCNGC	7 cut(s)	65, 68, 107, 313, 316, 1938, 1978
BmcAI	AGTACT	1 cut(s)	1840
Bme1390I	CCNGG	9 cut(s)	247, 549, 803, 906, 1798, 1799, 1893, 1894, 1941
Bme18I	GGWCC	4 cut(s)	908, 1469, 1772, 1889
BmeT110I	CYCGRG	2 cut(s)	1797, 1892
BmgBI	CACGTC	2 cut(s)	388, 1283
BmgT120I	GGNCC	8 cut(s)	631, 632, 908, 1469, 1558, 1559, 1772, 1889
BmrFI	CCNGG	9 cut(s)	247, 549, 803, 906, 1798, 1799, 1893, 1894, 1941
BmrI	ACTGGG	1 cut(s)	1624
BmsI	GCATC	7 cut(s)	406, 591, 627, 887, 909, 1042, 1935
BmuI	ACTGGG	1 cut(s)	1624
BoxI	GACNNNNGTC	1 cut(s)	2037
Bpu1102I	GCTNAGC	1 cut(s)	59
BpuEI	CTTGAG	1 cut(s)	1358
BpuMI	CCSGG	5 cut(s)	247, 1798, 1799, 1893, 1894
BsaJI	CCNNGG	9 cut(s)	87, 112, 394, 904, 1775, 1797, 1892, 1893, 1940
BsaXI	ACNNNNNCTCC	4 cut(s)	104, 134, 558, 588
Bsc4I	CCNNNNNNNGG	5 cut(s)	592, 1277, 1691, 1781, 1804
Bse1I	ACTGG	4 cut(s)	170, 1302, 1630, 1818
Bse21I	CCTNAGG	1 cut(s)	53
Bse3DI	GCAATG	1 cut(s)	426
BseBI	CCWGG	4 cut(s)	549, 803, 906, 1941
BseDI	CCNNGG	9 cut(s)	87, 112, 394, 904, 1775, 1797, 1892, 1893, 1940
BseGI	GGATG	8 cut(s)	129, 582, 900, 1029, 1033, 1926, 2050, 2077
BseLI	CCNNNNNNNGG	5 cut(s)	592, 1277, 1691, 1781, 1804
BseMI	GCAATG	1 cut(s)	426
BseMII	CTCAG	4 cut(s)	44, 50, 1004, 1059
BseNI	ACTGG	4 cut(s)	170, 1302, 1630, 1818
BseRI	GAGGAG	6 cut(s)	25, 109, 153, 346, 349, 364
BseSI	GKGCMC	4 cut(s)	635, 934, 1562, 1637
BseXI	GCAGC	3 cut(s)	53, 117, 1963
BseYI	CCCAGC	1 cut(s)	1004
BshFI	GGCC	6 cut(s)	633, 718, 887, 1560, 1695, 1939
BshNI	GGYRCC	1 cut(s)	1540
BsiHKAI	GWGCWC	3 cut(s)	75, 934, 1637
BsiHKCI	CYCGRG	2 cut(s)	1797, 1892
BsiSI	CCGG	3 cut(s)	247, 1798, 1893
BslFI	GGGAC	4 cut(s)	1482, 1875, 1936, 1958
BslI	CCNNNNNNNGG	5 cut(s)	592, 1277, 1691, 1781, 1804
BsmAI	GTCTC	3 cut(s)	48, 298, 1172
BsmBI	CGTCTC	1 cut(s)	298
BsmFI	GGGAC	4 cut(s)	1482, 1875, 1936, 1958
BsnI	GGCC	6 cut(s)	633, 718, 887, 1560, 1695, 1939
BsoBI	CYCGRG	2 cut(s)	1797, 1892
Bsp120I	GGGCCC	2 cut(s)	631, 1558
Bsp1286I	GDGCHC	5 cut(s)	75, 635, 934, 1562, 1637
Bsp143I	GATC	7 cut(s)	460, 865, 1194, 1318, 1428, 1524, 1801
Bsp1720I	GCTNAGC	1 cut(s)	59
Bsp19I	CCATGG	1 cut(s)	87
BspANI	GGCC	6 cut(s)	633, 718, 887, 1560, 1695, 1939
BspCNI	CTCAG	4 cut(s)	45, 51, 1005, 1058
BspMAI	CTGCAG	2 cut(s)	107, 1117
BspMI	ACCTGC	2 cut(s)	110, 919
BspPI	GGATC	1 cut(s)	1809
BspT107I	GGYRCC	1 cut(s)	1540
BsrBI	CCGCTC	2 cut(s)	243, 269
BsrDI	GCAATG	1 cut(s)	426
BsrI	ACTGG	4 cut(s)	170, 1302, 1630, 1818
BssECI	CCNNGG	9 cut(s)	87, 112, 394, 904, 1775, 1797, 1892, 1893, 1940
BssMI	GATC	7 cut(s)	460, 865, 1194, 1318, 1428, 1524, 1801
BssSI	CACGAG	3 cut(s)	1329, 1689, 1731
BssT1I	CCWWGG	2 cut(s)	87, 1775
Bst2BI	CACGAG	3 cut(s)	1329, 1689, 1731
Bst2UI	CCWGG	4 cut(s)	549, 803, 906, 1941
Bst4CI	ACNGT	7 cut(s)	792, 1240, 1272, 1534, 1756, 2011, 2039
Bst6I	CTCTTC	3 cut(s)	171, 191, 1438
BstAPI	GCANNNNNTGC	1 cut(s)	812
BstC8I	GCNNGC	3 cut(s)	71, 1002, 1657
BstDEI	CTNAG	5 cut(s)	53, 59, 491, 1013, 1045
BstDSI	CCRYGG	3 cut(s)	87, 112, 394
BstF5I	GGATG	8 cut(s)	129, 582, 900, 1029, 1033, 1926, 2050, 2077
BstHHI	GCGC	1 cut(s)	385
BstKTI	GATC	7 cut(s)	463, 868, 1197, 1321, 1431, 1527, 1804
BstMAI	GTCTC	3 cut(s)	48, 298, 1172
BstMBI	GATC	7 cut(s)	460, 865, 1194, 1318, 1428, 1524, 1801
BstMWI	GCNNNNNNNGC	4 cut(s)	114, 308, 380, 812
BstNI	CCWGG	4 cut(s)	549, 803, 906, 1941
BstNSI	RCATGY	3 cut(s)	1004, 1059, 1441
BstPAI	GACNNNNGTC	1 cut(s)	2037
BstSCI	CCNGG	9 cut(s)	245, 547, 801, 904, 1796, 1797, 1891, 1892, 1939
BstSFI	CTRYAG	3 cut(s)	103, 1113, 2035
BstSLI	GKGCMC	4 cut(s)	635, 934, 1562, 1637
BstV1I	GCAGC	3 cut(s)	53, 117, 1963
BstX2I	RGATCY	1 cut(s)	1194
BstXI	CCANNNNNNTGG	1 cut(s)	94
BstYI	RGATCY	1 cut(s)	1194
Bsu36I	CCTNAGG	1 cut(s)	53
BsuI	GTATCC	1 cut(s)	2111
BsuRI	GGCC	6 cut(s)	633, 718, 887, 1560, 1695, 1939
BtgI	CCRYGG	3 cut(s)	87, 112, 394
BtrI	CACGTC	2 cut(s)	388, 1283
BtsCI	GGATG	8 cut(s)	129, 582, 900, 1029, 1033, 1926, 2050, 2077
BtsI	GCAGTG	1 cut(s)	1737
BtsIMutI	CAGTG	7 cut(s)	797, 1245, 1530, 1637, 1737, 1811, 2016
BveI	ACCTGC	2 cut(s)	110, 919
Cac8I	GCNNGC	3 cut(s)	71, 1002, 1657
CfoI	GCGC	1 cut(s)	385
Cfr13I	GGNCC	8 cut(s)	631, 632, 908, 1469, 1558, 1559, 1772, 1889
Cfr9I	CCCGGG	2 cut(s)	1797, 1892
Csp6I	GTAC	5 cut(s)	1203, 1407, 1626, 1794, 1839
CviAII	CATG	7 cut(s)	88, 683, 1001, 1056, 1438, 1462, 1555
CviQI	GTAC	5 cut(s)	1203, 1407, 1626, 1794, 1839
DdeI	CTNAG	5 cut(s)	53, 59, 491, 1013, 1045
DpnI	GATC	7 cut(s)	462, 867, 1196, 1320, 1430, 1526, 1803
DpnII	GATC	7 cut(s)	460, 865, 1194, 1318, 1428, 1524, 1801
Eam1104I	CTCTTC	3 cut(s)	171, 191, 1438
EarI	CTCTTC	3 cut(s)	171, 191, 1438
Eco130I	CCWWGG	2 cut(s)	87, 1775
Eco147I	AGGCCT	1 cut(s)	718
Eco24I	GRGCYC	2 cut(s)	635, 1562
Eco47I	GGWCC	4 cut(s)	908, 1469, 1772, 1889
Eco57I	CTGAAG	2 cut(s)	318, 705
Eco81I	CCTNAGG	1 cut(s)	53
Eco88I	CYCGRG	2 cut(s)	1797, 1892
EcoO109I	RGGNCCY	3 cut(s)	631, 1469, 1559
EcoRI	GAATTC	1 cut(s)	1457
EcoRII	CCWGG	4 cut(s)	547, 801, 904, 1939
EcoT14I	CCWWGG	2 cut(s)	87, 1775
EcoT22I	ATGCAT	2 cut(s)	902, 1057
EcoT38I	GRGCYC	2 cut(s)	635, 1562
ErhI	CCWWGG	2 cut(s)	87, 1775
Esp3I	CGTCTC	1 cut(s)	298
FaeI	CATG	7 cut(s)	91, 686, 1004, 1059, 1441, 1465, 1558
FalI	AAGNNNNNCTT	4 cut(s)	170, 202, 591, 623
FaqI	GGGAC	4 cut(s)	1482, 1875, 1936, 1958
FatI	CATG	7 cut(s)	87, 682, 1000, 1055, 1437, 1461, 1554
FauI	CCCGC	3 cut(s)	282, 829, 1581
FauNDI	CATATG	1 cut(s)	1519
FblI	GTMKAC	1 cut(s)	390
Fnu4HI	GCNGC	7 cut(s)	64, 67, 106, 312, 315, 1937, 1977
FokI	GGATG	8 cut(s)	116, 569, 887, 1036, 1040, 1913, 2057, 2084
FriOI	GRGCYC	2 cut(s)	635, 1562
Fsp4HI	GCNGC	7 cut(s)	64, 67, 106, 312, 315, 1937, 1977
FspBI	CTAG	5 cut(s)	141, 464, 671, 816, 1857
GlaI	GCGC	1 cut(s)	384
GluI	GCNGC	7 cut(s)	64, 67, 106, 312, 315, 1937, 1977
GsaI	CCCAGC	1 cut(s)	1008
HaeIII	GGCC	6 cut(s)	633, 718, 887, 1560, 1695, 1939
HapII	CCGG	3 cut(s)	247, 1798, 1893
HhaI	GCGC	1 cut(s)	385
Hin1II	CATG	7 cut(s)	91, 686, 1004, 1059, 1441, 1465, 1558
Hin6I	GCGC	1 cut(s)	383
HinP1I	GCGC	1 cut(s)	383
HincII	GTYRAC	1 cut(s)	391
HindII	GTYRAC	1 cut(s)	391
HinfI	GANTC	8 cut(s)	78, 452, 521, 604, 620, 1155, 1744, 2014
HpaII	CCGG	3 cut(s)	247, 1798, 1893
HphI	GGTGA	6 cut(s)	187, 263, 1145, 1260, 1507, 1796
Hpy166II	GTNNAC	7 cut(s)	391, 932, 1205, 1400, 1635, 1706, 1811
Hpy188I	TCNGA	8 cut(s)	83, 298, 559, 1014, 1048, 1170, 1917, 2105
Hpy188III	TCNNGA	8 cut(s)	464, 671, 731, 1331, 1432, 2018, 2042, 2066
Hpy8I	GTNNAC	7 cut(s)	391, 932, 1205, 1400, 1635, 1706, 1811
Hpy99I	CGWCG	1 cut(s)	392
HpyCH4III	ACNGT	7 cut(s)	792, 1240, 1272, 1534, 1756, 2011, 2039
HpyCH4IV	ACGT	3 cut(s)	387, 994, 1282
HpyF10VI	GCNNNNNNNGC	4 cut(s)	114, 308, 380, 812
HpyF3I	CTNAG	5 cut(s)	53, 59, 491, 1013, 1045
HpySE526I	ACGT	3 cut(s)	387, 994, 1282
Hsp92II	CATG	7 cut(s)	91, 686, 1004, 1059, 1441, 1465, 1558
HspAI	GCGC	1 cut(s)	383
Kzo9I	GATC	7 cut(s)	460, 865, 1194, 1318, 1428, 1524, 1801
LmnI	GCTCC	4 cut(s)	122, 248, 293, 1647
Lsp1109I	GCAGC	3 cut(s)	53, 117, 1963
LweI	GCATC	7 cut(s)	406, 591, 627, 887, 909, 1042, 1935
MaeI	CTAG	5 cut(s)	141, 464, 671, 816, 1857
MaeII	ACGT	3 cut(s)	387, 994, 1282
MaeIII	GTNAC	4 cut(s)	946, 1297, 1343, 2086
MalI	GATC	7 cut(s)	462, 867, 1196, 1320, 1430, 1526, 1803
MbiI	CCGCTC	2 cut(s)	243, 269
MboI	GATC	7 cut(s)	460, 865, 1194, 1318, 1428, 1524, 1801
MfeI	CAATTG	1 cut(s)	1182
MflI	RGATCY	1 cut(s)	1194
MhlI	GDGCHC	5 cut(s)	75, 635, 934, 1562, 1637
MlyI	GAGTC	3 cut(s)	87, 446, 515
MmeI	TCCRAC	3 cut(s)	1258, 1709, 2019
Mph1103I	ATGCAT	2 cut(s)	902, 1057
MroXI	GAANNNNTTC	2 cut(s)	260, 603
MseI	TTAA	6 cut(s)	531, 702, 825, 1485, 1721, 1956
MslI	CAYNNNNRTG	1 cut(s)	92
MspA1I	CMGCKG	3 cut(s)	66, 848, 1936
MspI	CCGG	3 cut(s)	247, 1798, 1893
MspR9I	CCNGG	9 cut(s)	247, 549, 803, 906, 1798, 1799, 1893, 1894, 1941
MunI	CAATTG	1 cut(s)	1182
MvaI	CCWGG	4 cut(s)	549, 803, 906, 1941
MwoI	GCNNNNNNNGC	4 cut(s)	114, 308, 380, 812
NciI	CCSGG	5 cut(s)	247, 1798, 1799, 1893, 1894
NcoI	CCATGG	1 cut(s)	87
NdeI	CATATG	1 cut(s)	1519
NdeII	GATC	7 cut(s)	460, 865, 1194, 1318, 1428, 1524, 1801
NlaIII	CATG	7 cut(s)	91, 686, 1004, 1059, 1441, 1465, 1558
NmuCI	GTSAC	1 cut(s)	2086
NsiI	ATGCAT	2 cut(s)	902, 1057
NspI	RCATGY	3 cut(s)	1004, 1059, 1441
PaeI	GCATGC	1 cut(s)	1004
PceI	AGGCCT	1 cut(s)	718
PcsI	WCGNNNNNNNCGW	2 cut(s)	384, 1327
PdmI	GAANNNNTTC	2 cut(s)	260, 603
PfeI	GAWTC	5 cut(s)	604, 620, 1155, 1744, 2014
PflMI	CCANNNNNTGG	1 cut(s)	1691
PfoI	TCCNGGA	2 cut(s)	245, 547
PkrI	GCNGC	7 cut(s)	65, 68, 107, 313, 316, 1938, 1978
PleI	GAGTC	3 cut(s)	86, 446, 515
PpsI	GAGTC	3 cut(s)	86, 446, 515
PpuMI	RGGWCCY	1 cut(s)	1469
PshAI	GACNNNNGTC	1 cut(s)	2037
PshBI	ATTAAT	1 cut(s)	531
PsiI	TTATAA	1 cut(s)	516
Psp5II	RGGWCCY	1 cut(s)	1469
Psp6I	CCWGG	4 cut(s)	547, 801, 904, 1939
PspFI	CCCAGC	1 cut(s)	1004
PspGI	CCWGG	4 cut(s)	547, 801, 904, 1939
PspOMI	GGGCCC	2 cut(s)	631, 1558
PspPI	GGNCC	8 cut(s)	631, 632, 908, 1469, 1558, 1559, 1772, 1889
PspPPI	RGGWCCY	1 cut(s)	1469
PstI	CTGCAG	2 cut(s)	107, 1117
PsuI	RGATCY	1 cut(s)	1194
PvuII	CAGCTG	1 cut(s)	848
RsaI	GTAC	5 cut(s)	1204, 1408, 1627, 1795, 1840
RsaNI	GTAC	5 cut(s)	1203, 1407, 1626, 1794, 1839
RseI	CAYNNNNRTG	1 cut(s)	92
SalI	GTCGAC	1 cut(s)	389
SaqAI	TTAA	6 cut(s)	531, 702, 825, 1485, 1721, 1956
SatI	GCNGC	7 cut(s)	64, 67, 106, 312, 315, 1937, 1977
Sau3AI	GATC	7 cut(s)	460, 865, 1194, 1318, 1428, 1524, 1801
Sau96I	GGNCC	8 cut(s)	631, 632, 908, 1469, 1558, 1559, 1772, 1889
ScaI	AGTACT	1 cut(s)	1840
SchI	GAGTC	3 cut(s)	87, 446, 515
ScrFI	CCNGG	9 cut(s)	247, 549, 803, 906, 1798, 1799, 1893, 1894, 1941
SduI	GDGCHC	5 cut(s)	75, 635, 934, 1562, 1637
SfaNI	GCATC	7 cut(s)	406, 591, 627, 887, 909, 1042, 1935
SfcI	CTRYAG	3 cut(s)	103, 1113, 2035
SinI	GGWCC	4 cut(s)	908, 1469, 1772, 1889
SmaI	CCCGGG	2 cut(s)	1799, 1894
SmiMI	CAYNNNNRTG	1 cut(s)	92
SmlI	CTYRAG	1 cut(s)	1337
SmoI	CTYRAG	1 cut(s)	1337
SphI	GCATGC	1 cut(s)	1004
SseBI	AGGCCT	1 cut(s)	718
SspMI	CTAG	5 cut(s)	141, 464, 671, 816, 1857
StuI	AGGCCT	1 cut(s)	718
StyD4I	CCNGG	9 cut(s)	245, 547, 801, 904, 1796, 1797, 1891, 1892, 1939
StyI	CCWWGG	2 cut(s)	87, 1775
TaaI	ACNGT	7 cut(s)	792, 1240, 1272, 1534, 1756, 2011, 2039
TaiI	ACGT	3 cut(s)	390, 997, 1285
TaqI	TCGA	5 cut(s)	390, 524, 882, 1060, 1431
TatI	WGTACW	1 cut(s)	1838
TauI	GCSGC	4 cut(s)	66, 314, 317, 1939
TfiI	GAWTC	5 cut(s)	604, 620, 1155, 1744, 2014
Tru1I	TTAA	6 cut(s)	531, 702, 825, 1485, 1721, 1956
Tru9I	TTAA	6 cut(s)	531, 702, 825, 1485, 1721, 1956
TscAI	CASTG	7 cut(s)	797, 1245, 1537, 1637, 1744, 1818, 2016
TseFI	GTSAC	1 cut(s)	2086
TseI	GCWGC	3 cut(s)	66, 105, 1976
Tsp45I	GTSAC	1 cut(s)	2086
TspDTI	ATGAA	9 cut(s)	754, 801, 981, 1107, 1370, 1378, 1450, 1470, 2088
TspGWI	ACGGA	3 cut(s)	280, 411, 1979
TspMI	CCCGGG	2 cut(s)	1797, 1892
TspRI	CASTG	7 cut(s)	797, 1245, 1537, 1637, 1744, 1818, 2016
Van91I	CCANNNNNTGG	1 cut(s)	1691
VneI	GTGCAC	2 cut(s)	930, 1633
VpaK11BI	GGWCC	4 cut(s)	908, 1469, 1772, 1889
VspI	ATTAAT	1 cut(s)	531
XapI	RAATTY	4 cut(s)	674, 1097, 1457, 1680
XbaI	TCTAGA	2 cut(s)	463, 670
XceI	RCATGY	3 cut(s)	1004, 1059, 1441
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1937
XmaI	CCCGGG	2 cut(s)	1797, 1892
XmiI	GTMKAC	1 cut(s)	390
XmnI	GAANNNNTTC	2 cut(s)	260, 603
XspI	CTAG	5 cut(s)	141, 464, 671, 816, 1857
ZrmI	AGTACT	1 cut(s)	1840
Zsp2I	ATGCAT	2 cut(s)	902, 1057

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.