Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0007980

Physical Location & Seq

Genomic Coordinates Reverse (-)

22953004 .. 22961301

Gene Structure 8298 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023388

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2055 bp

ATGGAGATGGCATTGGCAACCACCACCTTCTGCAGCTCCTCCTCCTCCTCCTCCATTATCCCAAACCCTAACCCTAGAACCTCTTCTCTTCCACTCTCCAACCAACTCTTCGGTTTCAGCTTACCTTCTTCTTCCACCGCCAAAACCACCACACTCAAACTCCGCTCCCGAAACCCTGCCTCCTTCACTCCAACACCTATCAAATGCTCCGTTTCAGAAGCCACCGAAGCCGCCGCCCCCGTCGAGAAGAAGAAATTGATGAGGAGAAGTGACATAAGGAACATAGCCATTGTAGCCCATGTTGATCATGGAAAGACTACTCTGGTTGATTCCATGTTGAGGCAAGCAAAGGTGTTTCGCGATAACCAAGTTGTCCAAGAAAGGATAATGGACTCCAATGATATAGAGCGTGAAAGGGGGATTACAATTTTGAGTAAAAACACATCTATTACGTATAAAGACTCGAAAATTAATATCATTGATACTCCTGGCCATTCTGACTTTGGAGGGGAGGTCGAGCGCATCCTCAATATGGTGGAAGGAATTCTTTTAGTGGTAGATTCTGTTGAGGGCCCGATGCCACAGACAAGATTTGTTTTGAAGAAGGCTCTAGAATTCGGACATGCTGTCGTTGTTGTTGTCAATAAAATTGATAGGCCTTCCGCCCGTCCGGATTTTGTGATCAATTCCACTTTTGAACTCTTTATTGAACTAAATGCTACTGATGAACAGTGTGATTTTCAAGCAATATATGCTAGTGGGATTAAAGGAAAGGCGGGCTTATCGGCTGATAATCTGTCAGATGATCTTGGGCCCCTTTTTGAGGCCATAATGAGATGCATCCCTGGACCGGCCATTGAAGATGGTGCACTGCAAATGCTTGCTACAAATATCGAGTACGATGAGCATAAAGGTCGAATAGCTATTGGACGCTTGCATGCTGGGGAACTTCGGAAAGGAATGGATGTGAAGGTATGTACCTCAGAAGATTCCTGTCGGTTTGCTAAAGTTAGTGAGCTTTTTGTGTATGAGAAGTTCAATAGGGTTTCAGTAGACAGTGTAAAGGCTGGTGATATATGTGCTGTTTGTGGAATTAATGATATTCAGATTGGTGAGACAATTGCTGATAAGGCAGATGGGAAGCCATTACCTGCCATTAGGGTGGAGGAACCAACAGTGAAAATGGCTTTTGCTATAAACACTTCACCGTTTGTTGGGCGCGAGGGAAAGTATGTTACCAGTAGGAACTTGCGAGATCGGCTTTCTCGTGAGCTTGAGCGTAACTTGGCAATGAAAGTTGAAGATGGTGAAACAGCAGATACCTTCATTGTTAGTGGACGAGGCACTTTGCATATTACCATATTGATTGAGAACATGCGAAGAGAAGGATTTGAATTTATGGTCGGACCTCCCAAAGTTATTAACAAAAGGGTGAATGACAAGTTGCTGGAACCTTATGAGATTGCCACTGTAGAGGTGCCAGAAGAGCACATGGGTCCAGTCGTTGAACTTCTTGGCAGAAGGCGGGGGCAAATGATTGATATGCAGGGGCTTGGGTCAGAAGGCACAAACCTGATCAAATATAAGATTCCAACTCGTGGCCTTCTTGGTTTACGAAATGCAATTTTAACAGCTTCTCGTGGCACAGCTATTCTGAATACAATATTTGATAGCTATGGACCTTGGGCTGGTGATATGAGTACCCGGGATCTGGGTTCACTGGTTGCCTTTGAGGGTGGAACGAGTACTTCTTATGCCCTTTCTAGTTCACAGGATAGAGGACAGTTGTTTATTGGTCCTGGGGTGGATGTTTATAAAGGTCAGATAGTTGGCATCCATCAGCGGTCTGGGGACTTATCTCTTAATGTATGTAAAAAGAAGGCTGCAACAAACATCCGTTCCAACAAAGAACAATCAGTCATTCTTGATACCCCATTGGATTACAGTCTGGATGACTGCATTGAGTACATTCAAGAGGATGAACTGGTGGAGGTCACTCCATTGAGCATCCGGATGTGCAAAAACCCCAAGTTCTCAAAGAAATCATCAAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0031647 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050821 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

685

Amino Acids

75.11

Weight (kDa)

6.24

Isoelectric Point (pI)

40.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	91 - 282	9.8e-53	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	294 - 375	5.8e-09	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	305 - 375	1.8e-11	Elongation factor Tu domain 2
EFG_C	PF00679	481 - 567	2e-21	Elongation factor G C-terminus
BipA_C	PF21018	572 - 675	4.5e-38	TypA/BipA C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13650 AT5G13650
fragaria_vesca	FvH4_6g23530 FvH4_6g23530 FvH4_6g23530 FvH4_6g23530
malus_domestica	MD07G1028800.v1.1 MD12G1072500.v1.1 MD14G1069900.v1.1
prunus_persica	Prupe.7G064400_v2.0.a1
pyrus_communis	pycom12g06100 pycom12g06110 pycom14g05790
rosa_chinensis	RchiOBHm_Chr3g0481531
rosa_laevigata	RLG00000023388
rosa_multiflora	Rmu_sc0010846.1_g000003 Rmu_sc0040423.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400150 Rroxscaffold_6G00400350
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243700 Rh3BG278800 Rh3DG270600
rosa_wichuraiana	Rw3G021770

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1815
Acc36I	ACCTGC	1 cut(s)	1159
AccB1I	GGYRCC	1 cut(s)	1477
AccB7I	CCANNNNNTGG	1 cut(s)	1598
AccBSI	CCGCTC	1 cut(s)	165
AccI	GTMKAC	1 cut(s)	1053
AccII	CGCG	2 cut(s)	360, 1221
AccIII	TCCGGA	2 cut(s)	670, 2010
AciI	CCGC	8 cut(s)	138, 163, 231, 234, 663, 776, 1525, 1843
AclWI	GGATC	1 cut(s)	1716
AcoI	YGGCCR	2 cut(s)	490, 852
AcsI	RAATTY	3 cut(s)	543, 614, 1394
AfaI	GTAC	5 cut(s)	899, 979, 1702, 1747, 1967
AfiI	CCNNNNNNNGG	8 cut(s)	339, 532, 823, 850, 1214, 1598, 1688, 1711
AhdI	GACNNNNNGTC	1 cut(s)	626
AjnI	CCWGG	3 cut(s)	487, 844, 1798
AjuI	GAANNNNNNNTTGG	2 cut(s)	92, 124
AluBI	AGCT	8 cut(s)	36, 120, 923, 1018, 1273, 1634, 1649, 1674
AluI	AGCT	8 cut(s)	36, 120, 923, 1018, 1273, 1634, 1649, 1674
Alw21I	GWGCWC	2 cut(s)	871, 1491
Alw26I	GTCTC	1 cut(s)	1109
Alw44I	GTGCAC	1 cut(s)	867
AlwI	GGATC	1 cut(s)	1716
Ama87I	CYCGRG	1 cut(s)	1704
Aor13HI	TCCGGA	2 cut(s)	670, 2010
AoxI	GGCC	7 cut(s)	490, 571, 656, 812, 825, 852, 1600
ApaI	GGGCCC	2 cut(s)	575, 816
ApaLI	GTGCAC	1 cut(s)	867
ApeKI	GCWGC	2 cut(s)	33, 1883
ApoI	RAATTY	3 cut(s)	543, 614, 1394
AseI	ATTAAT	2 cut(s)	471, 1095
Asp700I	GAANNNNTTC	2 cut(s)	82, 543
AspLEI	GCGC	2 cut(s)	522, 1221
AspS9I	GGNCC	9 cut(s)	571, 572, 812, 813, 848, 1406, 1496, 1679, 1796
AsuC2I	CCSGG	2 cut(s)	1705, 1706
AsuHPI	GGTGA	6 cut(s)	1082, 1124, 1197, 1319, 1444, 1703
AvaI	CYCGRG	1 cut(s)	1704
AvaII	GGWCC	5 cut(s)	848, 1406, 1496, 1679, 1796
BaeGI	GKGCMC	3 cut(s)	575, 816, 871
BalI	TGGCCA	1 cut(s)	492
BanI	GGYRCC	1 cut(s)	1477
BanII	GRGCYC	2 cut(s)	575, 816
BauI	CACGAG	3 cut(s)	1266, 1596, 1638
Bbv12I	GWGCWC	2 cut(s)	871, 1491
BbvI	GCAGC	2 cut(s)	45, 1870
BccI	CCATC	4 cut(s)	857, 1130, 1298, 1845
BcgI	CGANNNNNNTGC	2 cut(s)	896, 930
BciT130I	CCWGG	3 cut(s)	489, 846, 1800
BclI	TGATCA	3 cut(s)	304, 681, 1575
BcnI	CCSGG	2 cut(s)	1705, 1706
BcoDI	GTCTC	1 cut(s)	1109
BfaI	CTAG	4 cut(s)	75, 611, 756, 1764
BfmI	CTRYAG	2 cut(s)	31, 1470
BfuAI	ACCTGC	1 cut(s)	1159
BisI	GCNGC	4 cut(s)	34, 231, 234, 1884
BlsI	GCNGC	4 cut(s)	35, 232, 235, 1885
BmcAI	AGTACT	1 cut(s)	1747
Bme1390I	CCNGG	5 cut(s)	489, 846, 1705, 1706, 1800
Bme18I	GGWCC	5 cut(s)	848, 1406, 1496, 1679, 1796
BmeRI	GACNNNNNGTC	1 cut(s)	626
BmeT110I	CYCGRG	1 cut(s)	1704
BmgT120I	GGNCC	9 cut(s)	571, 572, 812, 813, 848, 1406, 1496, 1679, 1796
BmiI	GGNNCC	7 cut(s)	573, 814, 815, 1170, 1452, 1479, 1497
BmrFI	CCNGG	5 cut(s)	489, 846, 1705, 1706, 1800
BmsI	GCATC	6 cut(s)	531, 567, 827, 849, 1842, 2016
BpuEI	CTTGAG	1 cut(s)	1295
BpuMI	CCSGG	2 cut(s)	1705, 1706
BsaAI	YACGTR	1 cut(s)	453
BsaJI	CCNNGG	4 cut(s)	844, 1682, 1704, 1799
BsaWI	WCCGGW	2 cut(s)	670, 2010
BsaXI	ACNNNNNCTCC	6 cut(s)	144, 164, 174, 194, 498, 528
Bsc4I	CCNNNNNNNGG	8 cut(s)	339, 532, 823, 850, 1214, 1598, 1688, 1711
Bse118I	RCCGGY	1 cut(s)	850
Bse1I	ACTGG	4 cut(s)	1239, 1499, 1725, 1989
Bse3DI	GCAATG	1 cut(s)	1296
BseAI	TCCGGA	2 cut(s)	670, 2010
BseBI	CCWGG	3 cut(s)	489, 846, 1800
BseDI	CCNNGG	4 cut(s)	844, 1682, 1704, 1799
BseLI	CCNNNNNNNGG	8 cut(s)	339, 532, 823, 850, 1214, 1598, 1688, 1711
BseMI	GCAATG	1 cut(s)	1296
BseMII	CTCAG	1 cut(s)	996
BseNI	ACTGG	4 cut(s)	1239, 1499, 1725, 1989
BseRI	GAGGAG	6 cut(s)	28, 31, 34, 37, 40, 277
BseSI	GKGCMC	3 cut(s)	575, 816, 871
BseXI	GCAGC	2 cut(s)	45, 1870
BseYI	CCCAGC	1 cut(s)	941
Bsh1236I	CGCG	2 cut(s)	360, 1221
BshFI	GGCC	7 cut(s)	492, 573, 658, 814, 827, 854, 1602
BshNI	GGYRCC	1 cut(s)	1477
BsiHKAI	GWGCWC	2 cut(s)	871, 1491
BsiHKCI	CYCGRG	1 cut(s)	1704
BsiSI	CCGG	4 cut(s)	671, 851, 1705, 2011
BslFI	GGGAC	1 cut(s)	1865
BslI	CCNNNNNNNGG	8 cut(s)	339, 532, 823, 850, 1214, 1598, 1688, 1711
BsmAI	GTCTC	1 cut(s)	1109
BsmFI	GGGAC	1 cut(s)	1865
BsnI	GGCC	7 cut(s)	492, 573, 658, 814, 827, 854, 1602
BsoBI	CYCGRG	1 cut(s)	1704
Bsp120I	GGGCCC	2 cut(s)	571, 812
Bsp1286I	GDGCHC	4 cut(s)	575, 816, 871, 1491
Bsp13I	TCCGGA	2 cut(s)	670, 2010
Bsp143I	GATC	6 cut(s)	304, 681, 805, 1255, 1575, 1708
Bsp68I	TCGCGA	1 cut(s)	360
BspACI	CCGC	8 cut(s)	138, 163, 231, 234, 663, 776, 1525, 1843
BspANI	GGCC	7 cut(s)	492, 573, 658, 814, 827, 854, 1602
BspCNI	CTCAG	1 cut(s)	995
BspEI	TCCGGA	2 cut(s)	670, 2010
BspFNI	CGCG	2 cut(s)	360, 1221
BspLI	GGNNCC	7 cut(s)	573, 814, 815, 1170, 1452, 1479, 1497
BspMAI	CTGCAG	1 cut(s)	35
BspMI	ACCTGC	1 cut(s)	1159
BspPI	GGATC	1 cut(s)	1716
BspQI	GCTCTTC	1 cut(s)	1479
BspT107I	GGYRCC	1 cut(s)	1477
BsrBI	CCGCTC	1 cut(s)	165
BsrDI	GCAATG	1 cut(s)	1296
BsrFI	RCCGGY	1 cut(s)	850
BsrI	ACTGG	4 cut(s)	1239, 1499, 1725, 1989
BssAI	RCCGGY	1 cut(s)	850
BssECI	CCNNGG	4 cut(s)	844, 1682, 1704, 1799
BssMI	GATC	6 cut(s)	304, 681, 805, 1255, 1575, 1708
BssSI	CACGAG	3 cut(s)	1266, 1596, 1638
BssT1I	CCWWGG	1 cut(s)	1682
Bst2BI	CACGAG	3 cut(s)	1266, 1596, 1638
Bst2UI	CCWGG	3 cut(s)	489, 846, 1800
Bst4CI	ACNGT	7 cut(s)	732, 1058, 1177, 1209, 1471, 1785, 1946
Bst6I	CTCTTC	5 cut(s)	88, 93, 113, 1375, 1479
BstAPI	GCANNNNNTGC	1 cut(s)	752
BstBAI	YACGTR	1 cut(s)	453
BstC8I	GCNNGC	5 cut(s)	345, 778, 882, 935, 939
BstDEI	CTNAG	1 cut(s)	982
BstENI	CCTNNNNNAGG	1 cut(s)	821
BstFNI	CGCG	2 cut(s)	360, 1221
BstHHI	GCGC	2 cut(s)	522, 1221
BstKTI	GATC	6 cut(s)	307, 684, 808, 1258, 1578, 1711
BstMAI	GTCTC	1 cut(s)	1109
BstMBI	GATC	6 cut(s)	304, 681, 805, 1255, 1575, 1708
BstMWI	GCNNNNNNNGC	6 cut(s)	227, 293, 752, 1130, 1258, 1486
BstNI	CCWGG	3 cut(s)	489, 846, 1800
BstNSI	RCATGY	3 cut(s)	626, 941, 1378
BstSCI	CCNGG	5 cut(s)	487, 844, 1703, 1704, 1798
BstSFI	CTRYAG	2 cut(s)	31, 1470
BstSLI	GKGCMC	3 cut(s)	575, 816, 871
BstSNI	TACGTA	1 cut(s)	453
BstUI	CGCG	2 cut(s)	360, 1221
BstV1I	GCAGC	2 cut(s)	45, 1870
BstX2I	RGATCY	1 cut(s)	1708
BstXI	CCANNNNNNTGG	1 cut(s)	1162
BstYI	RGATCY	1 cut(s)	1708
BsuRI	GGCC	7 cut(s)	492, 573, 658, 814, 827, 854, 1602
BtsI	GCAGTG	1 cut(s)	869
BtsIMutI	CAGTG	6 cut(s)	737, 869, 1063, 1182, 1467, 1718
BtuMI	TCGCGA	1 cut(s)	360
BveI	ACCTGC	1 cut(s)	1159
Cac8I	GCNNGC	5 cut(s)	345, 778, 882, 935, 939
CfoI	GCGC	2 cut(s)	522, 1221
Cfr10I	RCCGGY	1 cut(s)	850
Cfr13I	GGNCC	9 cut(s)	571, 572, 812, 813, 848, 1406, 1496, 1679, 1796
Cfr9I	CCCGGG	1 cut(s)	1704
CseI	GACGC	1 cut(s)	939
Csp6I	GTAC	5 cut(s)	898, 978, 1701, 1746, 1966
CviAII	CATG	7 cut(s)	299, 308, 334, 623, 938, 1375, 1492
CviQI	GTAC	5 cut(s)	898, 978, 1701, 1746, 1966
DdeI	CTNAG	1 cut(s)	982
DpnI	GATC	6 cut(s)	306, 683, 807, 1257, 1577, 1710
DpnII	GATC	6 cut(s)	304, 681, 805, 1255, 1575, 1708
DriI	GACNNNNNGTC	1 cut(s)	626
EaeI	YGGCCR	2 cut(s)	490, 852
Eam1104I	CTCTTC	5 cut(s)	88, 93, 113, 1375, 1479
Eam1105I	GACNNNNNGTC	1 cut(s)	626
EarI	CTCTTC	5 cut(s)	88, 93, 113, 1375, 1479
EciI	GGCGGA	1 cut(s)	652
Eco105I	TACGTA	1 cut(s)	453
Eco130I	CCWWGG	1 cut(s)	1682
Eco147I	AGGCCT	1 cut(s)	658
Eco24I	GRGCYC	2 cut(s)	575, 816
Eco47I	GGWCC	5 cut(s)	848, 1406, 1496, 1679, 1796
Eco88I	CYCGRG	1 cut(s)	1704
EcoNI	CCTNNNNNAGG	1 cut(s)	821
EcoO109I	RGGNCCY	2 cut(s)	571, 813
EcoRI	GAATTC	2 cut(s)	543, 614
EcoRII	CCWGG	3 cut(s)	487, 844, 1798
EcoT14I	CCWWGG	1 cut(s)	1682
EcoT22I	ATGCAT	1 cut(s)	842
EcoT38I	GRGCYC	2 cut(s)	575, 816
ErhI	CCWWGG	1 cut(s)	1682
FaeI	CATG	7 cut(s)	302, 311, 337, 626, 941, 1378, 1495
FalI	AAGNNNNNCTT	4 cut(s)	531, 563, 764, 796
FaqI	GGGAC	1 cut(s)	1865
FatI	CATG	7 cut(s)	298, 307, 333, 622, 937, 1374, 1491
FauI	CCCGC	2 cut(s)	769, 1518
FbaI	TGATCA	3 cut(s)	304, 681, 1575
FblI	GTMKAC	1 cut(s)	1053
Fnu4HI	GCNGC	4 cut(s)	34, 231, 234, 1884
FriOI	GRGCYC	2 cut(s)	575, 816
Fsp4HI	GCNGC	4 cut(s)	34, 231, 234, 1884
FspBI	CTAG	4 cut(s)	75, 611, 756, 1764
GlaI	GCGC	2 cut(s)	521, 1220
GluI	GCNGC	4 cut(s)	34, 231, 234, 1884
GsaI	CCCAGC	1 cut(s)	945
HaeIII	GGCC	7 cut(s)	492, 573, 658, 814, 827, 854, 1602
HapII	CCGG	4 cut(s)	671, 851, 1705, 2011
HgaI	GACGC	1 cut(s)	939
HhaI	GCGC	2 cut(s)	522, 1221
Hin1II	CATG	7 cut(s)	302, 311, 337, 626, 941, 1378, 1495
Hin6I	GCGC	2 cut(s)	520, 1219
HinP1I	GCGC	2 cut(s)	520, 1219
HinfI	GANTC	6 cut(s)	329, 392, 461, 560, 989, 1588
HpaII	CCGG	4 cut(s)	671, 851, 1705, 2011
HphI	GGTGA	6 cut(s)	1082, 1124, 1197, 1319, 1444, 1703
Hpy166II	GTNNAC	6 cut(s)	869, 1054, 1337, 1613, 1718, 1769
Hpy8I	GTNNAC	6 cut(s)	869, 1054, 1337, 1613, 1718, 1769
Hpy99I	CGWCG	1 cut(s)	245
HpyCH4III	ACNGT	7 cut(s)	732, 1058, 1177, 1209, 1471, 1785, 1946
HpyCH4IV	ACGT	1 cut(s)	452
HpyF10VI	GCNNNNNNNGC	6 cut(s)	227, 293, 752, 1130, 1258, 1486
HpyF3I	CTNAG	1 cut(s)	982
HpySE526I	ACGT	1 cut(s)	452
Hsp92II	CATG	7 cut(s)	302, 311, 337, 626, 941, 1378, 1495
HspAI	GCGC	2 cut(s)	520, 1219
Kpn2I	TCCGGA	2 cut(s)	670, 2010
Ksp22I	TGATCA	3 cut(s)	304, 681, 1575
Kzo9I	GATC	6 cut(s)	304, 681, 805, 1255, 1575, 1708
LguI	GCTCTTC	1 cut(s)	1479
LmnI	GCTCC	3 cut(s)	41, 170, 212
Lsp1109I	GCAGC	2 cut(s)	45, 1870
LweI	GCATC	6 cut(s)	531, 567, 827, 849, 1842, 2016
MaeI	CTAG	4 cut(s)	75, 611, 756, 1764
MaeII	ACGT	1 cut(s)	452
MaeIII	GTNAC	4 cut(s)	269, 1234, 1280, 1993
MalI	GATC	6 cut(s)	306, 683, 807, 1257, 1577, 1710
MbiI	CCGCTC	1 cut(s)	165
MboI	GATC	6 cut(s)	304, 681, 805, 1255, 1575, 1708
MfeI	CAATTG	1 cut(s)	1119
MflI	RGATCY	1 cut(s)	1708
MhlI	GDGCHC	4 cut(s)	575, 816, 871, 1491
MlsI	TGGCCA	1 cut(s)	492
MluNI	TGGCCA	1 cut(s)	492
MlyI	GAGTC	2 cut(s)	386, 455
MmeI	TCCRAC	5 cut(s)	123, 215, 1384, 1616, 1926
Mox20I	TGGCCA	1 cut(s)	492
Mph1103I	ATGCAT	1 cut(s)	842
MroI	TCCGGA	2 cut(s)	670, 2010
MroXI	GAANNNNTTC	2 cut(s)	82, 543
MscI	TGGCCA	1 cut(s)	492
MseI	TTAA	6 cut(s)	471, 765, 1095, 1422, 1628, 1863
MslI	CAYNNNNRTG	2 cut(s)	1160, 2012
Msp20I	TGGCCA	1 cut(s)	492
MspA1I	CMGCKG	1 cut(s)	1843
MspI	CCGG	4 cut(s)	671, 851, 1705, 2011
MspR9I	CCNGG	5 cut(s)	489, 846, 1705, 1706, 1800
MunI	CAATTG	1 cut(s)	1119
MvaI	CCWGG	3 cut(s)	489, 846, 1800
MvnI	CGCG	2 cut(s)	360, 1221
MwoI	GCNNNNNNNGC	6 cut(s)	227, 293, 752, 1130, 1258, 1486
NciI	CCSGG	2 cut(s)	1705, 1706
NdeII	GATC	6 cut(s)	304, 681, 805, 1255, 1575, 1708
NlaIII	CATG	7 cut(s)	302, 311, 337, 626, 941, 1378, 1495
NlaIV	GGNNCC	7 cut(s)	573, 814, 815, 1170, 1452, 1479, 1497
NmuCI	GTSAC	2 cut(s)	269, 1993
NruI	TCGCGA	1 cut(s)	360
NsiI	ATGCAT	1 cut(s)	842
NspI	RCATGY	3 cut(s)	626, 941, 1378
PaeI	GCATGC	1 cut(s)	941
PceI	AGGCCT	1 cut(s)	658
PciSI	GCTCTTC	1 cut(s)	1479
PcsI	WCGNNNNNNNCGW	1 cut(s)	1264
PdmI	GAANNNNTTC	2 cut(s)	82, 543
PfeI	GAWTC	4 cut(s)	329, 560, 989, 1588
PflMI	CCANNNNNTGG	1 cut(s)	1598
PkrI	GCNGC	4 cut(s)	35, 232, 235, 1885
PleI	GAGTC	2 cut(s)	386, 455
PpsI	GAGTC	2 cut(s)	386, 455
Ppu21I	YACGTR	1 cut(s)	453
PshBI	ATTAAT	2 cut(s)	471, 1095
PsiI	TTATAA	1 cut(s)	1815
Psp6I	CCWGG	3 cut(s)	487, 844, 1798
PspFI	CCCAGC	1 cut(s)	941
PspGI	CCWGG	3 cut(s)	487, 844, 1798
PspN4I	GGNNCC	7 cut(s)	573, 814, 815, 1170, 1452, 1479, 1497
PspOMI	GGGCCC	2 cut(s)	571, 812
PspPI	GGNCC	9 cut(s)	571, 572, 812, 813, 848, 1406, 1496, 1679, 1796
PsrI	GAACNNNNNNTAC	2 cut(s)	1019, 1051
PstI	CTGCAG	1 cut(s)	35
PsuI	RGATCY	1 cut(s)	1708
RruI	TCGCGA	1 cut(s)	360
RsaI	GTAC	5 cut(s)	899, 979, 1702, 1747, 1967
RsaNI	GTAC	5 cut(s)	898, 978, 1701, 1746, 1966
RseI	CAYNNNNRTG	2 cut(s)	1160, 2012
SapI	GCTCTTC	1 cut(s)	1479
SaqAI	TTAA	6 cut(s)	471, 765, 1095, 1422, 1628, 1863
SatI	GCNGC	4 cut(s)	34, 231, 234, 1884
Sau3AI	GATC	6 cut(s)	304, 681, 805, 1255, 1575, 1708
Sau96I	GGNCC	9 cut(s)	571, 572, 812, 813, 848, 1406, 1496, 1679, 1796
ScaI	AGTACT	1 cut(s)	1747
SchI	GAGTC	2 cut(s)	386, 455
ScrFI	CCNGG	5 cut(s)	489, 846, 1705, 1706, 1800
SduI	GDGCHC	4 cut(s)	575, 816, 871, 1491
SfaNI	GCATC	6 cut(s)	531, 567, 827, 849, 1842, 2016
SfcI	CTRYAG	2 cut(s)	31, 1470
SinI	GGWCC	5 cut(s)	848, 1406, 1496, 1679, 1796
SmaI	CCCGGG	1 cut(s)	1706
SmiMI	CAYNNNNRTG	2 cut(s)	1160, 2012
SmlI	CTYRAG	1 cut(s)	1274
SmoI	CTYRAG	1 cut(s)	1274
SnaBI	TACGTA	1 cut(s)	453
SphI	GCATGC	1 cut(s)	941
SseBI	AGGCCT	1 cut(s)	658
SsiI	CCGC	8 cut(s)	138, 163, 231, 234, 663, 776, 1525, 1843
SspI	AATATT	1 cut(s)	1665
SspMI	CTAG	4 cut(s)	75, 611, 756, 1764
StuI	AGGCCT	1 cut(s)	658
StyD4I	CCNGG	5 cut(s)	487, 844, 1703, 1704, 1798
StyI	CCWWGG	1 cut(s)	1682
TaaI	ACNGT	7 cut(s)	732, 1058, 1177, 1209, 1471, 1785, 1946
TaiI	ACGT	1 cut(s)	455
TaqI	TCGA	5 cut(s)	243, 464, 516, 894, 916
TatI	WGTACW	2 cut(s)	1745, 1965
TauI	GCSGC	2 cut(s)	233, 236
TfiI	GAWTC	4 cut(s)	329, 560, 989, 1588
Tru1I	TTAA	6 cut(s)	471, 765, 1095, 1422, 1628, 1863
Tru9I	TTAA	6 cut(s)	471, 765, 1095, 1422, 1628, 1863
TscAI	CASTG	6 cut(s)	737, 876, 1063, 1182, 1474, 1725
TseFI	GTSAC	2 cut(s)	269, 1993
TseI	GCWGC	2 cut(s)	33, 1883
Tsp45I	GTSAC	2 cut(s)	269, 1993
TspDTI	ATGAA	4 cut(s)	741, 1307, 1315, 1995
TspGWI	ACGGA	2 cut(s)	199, 1886
TspMI	CCCGGG	1 cut(s)	1704
TspRI	CASTG	6 cut(s)	737, 876, 1063, 1182, 1474, 1725
Van91I	CCANNNNNTGG	1 cut(s)	1598
VneI	GTGCAC	1 cut(s)	867
VpaK11BI	GGWCC	5 cut(s)	848, 1406, 1496, 1679, 1796
VspI	ATTAAT	2 cut(s)	471, 1095
XagI	CCTNNNNNAGG	1 cut(s)	821
XapI	RAATTY	3 cut(s)	543, 614, 1394
XbaI	TCTAGA	1 cut(s)	610
XceI	RCATGY	3 cut(s)	626, 941, 1378
XcmI	CCANNNNNNNNNTGG	3 cut(s)	305, 500, 1844
XmaI	CCCGGG	1 cut(s)	1704
XmiI	GTMKAC	1 cut(s)	1053
XmnI	GAANNNNTTC	2 cut(s)	82, 543
XspI	CTAG	4 cut(s)	75, 611, 756, 1764
ZrmI	AGTACT	1 cut(s)	1747
Zsp2I	ATGCAT	1 cut(s)	842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.