Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0007980

Physical Location & Seq

Genomic Coordinates Forward (+)

27081540 .. 27089463

Gene Structure 7924 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG270600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGGAGATGGCAACCTGCTCCTCCTTCTCCTTCTCTATCCCAAACCCTAACCCTAACCCTAGAACCTCTTCCCTTCCACTCTCCAAACAGCTCTTCGGTTTCAGCTTGCCTTCTTCTTCCACCGCCAAAACCACCACACTCAAACTCCGCTCCCGAAACCCTACCTCCTTCACTCCAACACCTATCAAATGCTCCGTCTCAGAAGCCACCGCCCCCGTCGAGAAGAAGAAATTGATGAGGAGAAGTGACATAAGGAACATAGCCATTGTAGCCCATGTTGATCATGGAAAGACAACTCTGGTTGATTCCATGTTGAGGCAAGCAAAGGTGTTTCGCGATAACCAAGTTGTCCAAGAAAGGATAATGGACTCCAATGATATAGAGCGTGAAAGGGGGATTACAATTTTGAGTAAAAACACATCTATTACGTATAAAGACTCGAAAATTAATATCATTGATACTCCTGGCCATTCTGACTTTGGAGGGGAGGTCGAGCGCATCCTCAATATGGTGGAAGGGATTCTTTTAGTGGTAGATTCTGTTGAGGGCCCGATGCCACAGACAAGATTTGTTTTGAAGAAGGCTCTGGAATTCGGACACGCTGTCGTCGTTGTTGTCAATAAAATTGATAGGCCTTCCGCCCGTCCGGATTTTGTGATCAATTCCACTTTTGAACTCTTTATTGAACTAAATGCCACTGATGAACAGTGTGATTTTCAAGCAATATATGCTAGTGGGATTAAAGGAAAGGCGGGCTTATCGGCTGATAATCTGTCAGATGACCTTGGGCCCCTTTTTGAGGCCATAATGAGATGCATCCCTGGACCGGCCATTGAAGATGGTGCACTGCAAATGCTTGCTACAAACATCGAGTACGATGAGCATAAAGGTAGAATAGCTATTGGACGCTTGCATGCTGGGGAACTTCGGAAAGGAATGGATGTGAAGGTATGTACCTCAGAAGATTCCTGTCGGTTTGCTAAAGTTAGTGAGCTTTTTGTGTATGAGAAGTTCAATAGGGTTTCAGTAGACAGTGTAAAGGCTGGTGATATATGTGCTGTTTGTGGAATTAATGATATTCAGATTGGTGAGACAATTGCTGATAAGACAGATGGGAAGCCATTACCTGCCATTAGGGTGGAGGAACCAACAGTGAAAATGGCGTTCGCTATAAACACTTCACCATTTGTTGGGCGCGAGGGAAAGTATGTTACCAGTAGGAACTTGCGAGATCGGCTTTCTCGTGAGCTTGAGCGTAACTTGGCAATGAAAGTTGAAGATGGTGAAACAGCAGATACCTTCATTGTTAGTGGACGAGGCACTTTGCATATTACCATATTGATTGAGAACATGCGAAGAGAAGGATTTGAATTTATGGTCGGACCTCCCAAAGTTATTAACAAAAGGGTGAATGACAAGTTGCTGGAACCTTATGAGATTGCCACTGTAGAGGTGCCAGAAGAGCACATGGGTCCAGTCGTTGAACTTCTTGGCAAAAGGCGGGGGCAAATGATTGATATGCAGGGGCTTGGGTCAGAAGGCACAAACCTGATCAAATATAAGATTCCAACTCGTGGCCTTCTTGGTTTACGAAATGCAATTTTAACAGCTTCTCGTGGCACAGCTATTCTGAATACAATATTTGATAGCTATGGACCTTGGGCTGGTGATATGAGTACCCGGGATCTGGGTTCACTGGTTGCCTTTGAGGGTGGAACGAGTACTTCTTATGCCCTTTCTAGTTCACAGGATAGAGGACAGTTGTTTATTGGTCCTGGGGTGGATGTTTATAAAGGTCAGATAGTTGGCATCCATCAGCGGTCTGGGGACTTATCTCTTAACGTATGTAAAAAGAAGGCTGCAACAAACATCCGTTCCAACAAAGAACAATCAGTCATTCTTGATACCCCATTGGATTACAGTCTGGATGACTGCATTGAGTACATTCAAGAGGATGAACTGGTGGAGGTCACTCCATTGAGCATCCGGATGTGCAAAAACCCCAAGTTCTCAAAGAAATCATCAAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0031647 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050821 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

676

Amino Acids

74.48

Weight (kDa)

6.57

Isoelectric Point (pI)

39.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	83 - 274	9.6e-53	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	286 - 367	5.7e-09	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	297 - 367	1.8e-11	Elongation factor Tu domain 2
EFG_C	PF00679	473 - 559	2.1e-21	Elongation factor G C-terminus
BipA_C	PF21018	564 - 667	4.4e-38	TypA/BipA C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13650 AT5G13650
fragaria_vesca	FvH4_6g23530 FvH4_6g23530 FvH4_6g23530 FvH4_6g23530
malus_domestica	MD07G1028800.v1.1 MD12G1072500.v1.1 MD14G1069900.v1.1
prunus_persica	Prupe.7G064400_v2.0.a1
pyrus_communis	pycom12g06100 pycom12g06110 pycom14g05790
rosa_chinensis	RchiOBHm_Chr3g0481531
rosa_laevigata	RLG00000023388
rosa_multiflora	Rmu_sc0010846.1_g000003 Rmu_sc0040423.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400150 Rroxscaffold_6G00400350
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243700 Rh3BG278800 Rh3DG270600
rosa_wichuraiana	Rw3G021770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1791
Acc36I	ACCTGC	2 cut(s)	23, 1135
AccB1I	GGYRCC	1 cut(s)	1453
AccB7I	CCANNNNNTGG	2 cut(s)	1190, 1574
AccBSI	CCGCTC	1 cut(s)	150
AccI	GTMKAC	1 cut(s)	1029
AccII	CGCG	2 cut(s)	336, 1197
AccIII	TCCGGA	2 cut(s)	646, 1986
AciI	CCGC	7 cut(s)	123, 148, 210, 639, 752, 1501, 1819
AclWI	GGATC	1 cut(s)	1692
AcoI	YGGCCR	2 cut(s)	466, 828
AcsI	RAATTY	2 cut(s)	590, 1370
AfaI	GTAC	5 cut(s)	875, 955, 1678, 1723, 1943
AfiI	CCNNNNNNNGG	8 cut(s)	315, 508, 799, 826, 1190, 1574, 1664, 1687
AhdI	GACNNNNNGTC	1 cut(s)	602
AjnI	CCWGG	3 cut(s)	463, 820, 1774
AjuI	GAANNNNNNNTTGG	2 cut(s)	77, 109
AluBI	AGCT	8 cut(s)	91, 105, 899, 994, 1249, 1610, 1625, 1650
AluI	AGCT	8 cut(s)	91, 105, 899, 994, 1249, 1610, 1625, 1650
Alw21I	GWGCWC	2 cut(s)	847, 1467
Alw26I	GTCTC	2 cut(s)	202, 1085
Alw44I	GTGCAC	1 cut(s)	843
AlwI	GGATC	1 cut(s)	1692
Ama87I	CYCGRG	1 cut(s)	1680
Aor13HI	TCCGGA	2 cut(s)	646, 1986
AoxI	GGCC	7 cut(s)	466, 547, 632, 788, 801, 828, 1576
ApaI	GGGCCC	2 cut(s)	551, 792
ApaLI	GTGCAC	1 cut(s)	843
ApeKI	GCWGC	1 cut(s)	1859
ApoI	RAATTY	2 cut(s)	590, 1370
ArsI	GACNNNNNNTTYG	2 cut(s)	180, 212
AseI	ATTAAT	2 cut(s)	447, 1071
Asp700I	GAANNNNTTC	2 cut(s)	67, 519
AspLEI	GCGC	2 cut(s)	498, 1197
AspS9I	GGNCC	9 cut(s)	547, 548, 788, 789, 824, 1382, 1472, 1655, 1772
AsuC2I	CCSGG	2 cut(s)	1681, 1682
AsuHPI	GGTGA	6 cut(s)	1058, 1100, 1173, 1295, 1420, 1679
AvaI	CYCGRG	1 cut(s)	1680
AvaII	GGWCC	5 cut(s)	824, 1382, 1472, 1655, 1772
BaeGI	GKGCMC	3 cut(s)	551, 792, 847
BalI	TGGCCA	1 cut(s)	468
BanI	GGYRCC	1 cut(s)	1453
BanII	GRGCYC	2 cut(s)	551, 792
BauI	CACGAG	3 cut(s)	1242, 1572, 1614
Bbv12I	GWGCWC	2 cut(s)	847, 1467
BbvI	GCAGC	1 cut(s)	1846
BccI	CCATC	4 cut(s)	833, 1106, 1274, 1821
BciT130I	CCWGG	3 cut(s)	465, 822, 1776
BclI	TGATCA	3 cut(s)	280, 657, 1551
BcnI	CCSGG	2 cut(s)	1681, 1682
BcoDI	GTCTC	2 cut(s)	202, 1085
BfaI	CTAG	3 cut(s)	60, 732, 1740
BfmI	CTRYAG	1 cut(s)	1446
BfuAI	ACCTGC	2 cut(s)	23, 1135
BisI	GCNGC	1 cut(s)	1860
BlsI	GCNGC	1 cut(s)	1861
BmcAI	AGTACT	1 cut(s)	1723
Bme1390I	CCNGG	5 cut(s)	465, 822, 1681, 1682, 1776
Bme18I	GGWCC	5 cut(s)	824, 1382, 1472, 1655, 1772
BmeRI	GACNNNNNGTC	1 cut(s)	602
BmeT110I	CYCGRG	1 cut(s)	1680
BmgT120I	GGNCC	9 cut(s)	547, 548, 788, 789, 824, 1382, 1472, 1655, 1772
BmiI	GGNNCC	7 cut(s)	549, 790, 791, 1146, 1428, 1455, 1473
BmrFI	CCNGG	5 cut(s)	465, 822, 1681, 1682, 1776
BmsI	GCATC	6 cut(s)	507, 543, 803, 825, 1818, 1992
BpuEI	CTTGAG	1 cut(s)	1271
BpuMI	CCSGG	2 cut(s)	1681, 1682
BsaAI	YACGTR	1 cut(s)	429
BsaJI	CCNNGG	5 cut(s)	784, 820, 1658, 1680, 1775
BsaWI	WCCGGW	2 cut(s)	646, 1986
BsaXI	ACNNNNNCTCC	6 cut(s)	129, 149, 159, 179, 474, 504
Bsc4I	CCNNNNNNNGG	8 cut(s)	315, 508, 799, 826, 1190, 1574, 1664, 1687
Bse118I	RCCGGY	1 cut(s)	826
Bse1I	ACTGG	4 cut(s)	1215, 1475, 1701, 1965
Bse3DI	GCAATG	1 cut(s)	1272
BseAI	TCCGGA	2 cut(s)	646, 1986
BseBI	CCWGG	3 cut(s)	465, 822, 1776
BseDI	CCNNGG	5 cut(s)	784, 820, 1658, 1680, 1775
BseLI	CCNNNNNNNGG	8 cut(s)	315, 508, 799, 826, 1190, 1574, 1664, 1687
BseMI	GCAATG	1 cut(s)	1272
BseMII	CTCAG	2 cut(s)	213, 972
BseNI	ACTGG	4 cut(s)	1215, 1475, 1701, 1965
BseRI	GAGGAG	2 cut(s)	10, 253
BseSI	GKGCMC	3 cut(s)	551, 792, 847
BseXI	GCAGC	1 cut(s)	1846
BseYI	CCCAGC	1 cut(s)	917
Bsh1236I	CGCG	2 cut(s)	336, 1197
BshFI	GGCC	7 cut(s)	468, 549, 634, 790, 803, 830, 1578
BshNI	GGYRCC	1 cut(s)	1453
BsiHKAI	GWGCWC	2 cut(s)	847, 1467
BsiHKCI	CYCGRG	1 cut(s)	1680
BsiSI	CCGG	4 cut(s)	647, 827, 1681, 1987
BslFI	GGGAC	1 cut(s)	1841
BslI	CCNNNNNNNGG	8 cut(s)	315, 508, 799, 826, 1190, 1574, 1664, 1687
BsmAI	GTCTC	2 cut(s)	202, 1085
BsmBI	CGTCTC	1 cut(s)	202
BsmFI	GGGAC	1 cut(s)	1841
BsnI	GGCC	7 cut(s)	468, 549, 634, 790, 803, 830, 1578
BsoBI	CYCGRG	1 cut(s)	1680
Bsp120I	GGGCCC	2 cut(s)	547, 788
Bsp1286I	GDGCHC	4 cut(s)	551, 792, 847, 1467
Bsp13I	TCCGGA	2 cut(s)	646, 1986
Bsp143I	GATC	5 cut(s)	280, 657, 1231, 1551, 1684
Bsp68I	TCGCGA	1 cut(s)	336
BspACI	CCGC	7 cut(s)	123, 148, 210, 639, 752, 1501, 1819
BspANI	GGCC	7 cut(s)	468, 549, 634, 790, 803, 830, 1578
BspCNI	CTCAG	2 cut(s)	212, 971
BspEI	TCCGGA	2 cut(s)	646, 1986
BspFNI	CGCG	2 cut(s)	336, 1197
BspLI	GGNNCC	7 cut(s)	549, 790, 791, 1146, 1428, 1455, 1473
BspMI	ACCTGC	2 cut(s)	23, 1135
BspPI	GGATC	1 cut(s)	1692
BspQI	GCTCTTC	2 cut(s)	98, 1455
BspT107I	GGYRCC	1 cut(s)	1453
BsrBI	CCGCTC	1 cut(s)	150
BsrDI	GCAATG	1 cut(s)	1272
BsrFI	RCCGGY	1 cut(s)	826
BsrI	ACTGG	4 cut(s)	1215, 1475, 1701, 1965
BssAI	RCCGGY	1 cut(s)	826
BssECI	CCNNGG	5 cut(s)	784, 820, 1658, 1680, 1775
BssMI	GATC	5 cut(s)	280, 657, 1231, 1551, 1684
BssSI	CACGAG	3 cut(s)	1242, 1572, 1614
BssT1I	CCWWGG	2 cut(s)	784, 1658
Bst2BI	CACGAG	3 cut(s)	1242, 1572, 1614
Bst2UI	CCWGG	3 cut(s)	465, 822, 1776
Bst4CI	ACNGT	6 cut(s)	708, 1034, 1153, 1447, 1761, 1922
Bst6I	CTCTTC	4 cut(s)	73, 98, 1351, 1455
BstAPI	GCANNNNNTGC	1 cut(s)	728
BstBAI	YACGTR	1 cut(s)	429
BstC8I	GCNNGC	6 cut(s)	107, 321, 754, 858, 911, 915
BstDEI	CTNAG	2 cut(s)	199, 958
BstENI	CCTNNNNNAGG	1 cut(s)	797
BstFNI	CGCG	2 cut(s)	336, 1197
BstHHI	GCGC	2 cut(s)	498, 1197
BstKTI	GATC	5 cut(s)	283, 660, 1234, 1554, 1687
BstMAI	GTCTC	2 cut(s)	202, 1085
BstMBI	GATC	5 cut(s)	280, 657, 1231, 1551, 1684
BstMWI	GCNNNNNNNGC	4 cut(s)	269, 728, 1234, 1462
BstNI	CCWGG	3 cut(s)	465, 822, 1776
BstNSI	RCATGY	2 cut(s)	917, 1354
BstSCI	CCNGG	5 cut(s)	463, 820, 1679, 1680, 1774
BstSFI	CTRYAG	1 cut(s)	1446
BstSLI	GKGCMC	3 cut(s)	551, 792, 847
BstSNI	TACGTA	1 cut(s)	429
BstUI	CGCG	2 cut(s)	336, 1197
BstV1I	GCAGC	1 cut(s)	1846
BstX2I	RGATCY	1 cut(s)	1684
BstXI	CCANNNNNNTGG	1 cut(s)	1138
BstYI	RGATCY	1 cut(s)	1684
BsuRI	GGCC	7 cut(s)	468, 549, 634, 790, 803, 830, 1578
BtsI	GCAGTG	1 cut(s)	845
BtsIMutI	CAGTG	7 cut(s)	696, 713, 845, 1039, 1158, 1443, 1694
BtuMI	TCGCGA	1 cut(s)	336
BveI	ACCTGC	2 cut(s)	23, 1135
Cac8I	GCNNGC	6 cut(s)	107, 321, 754, 858, 911, 915
CfoI	GCGC	2 cut(s)	498, 1197
Cfr10I	RCCGGY	1 cut(s)	826
Cfr13I	GGNCC	9 cut(s)	547, 548, 788, 789, 824, 1382, 1472, 1655, 1772
Cfr9I	CCCGGG	1 cut(s)	1680
CseI	GACGC	1 cut(s)	915
Csp6I	GTAC	5 cut(s)	874, 954, 1677, 1722, 1942
CviAII	CATG	6 cut(s)	275, 284, 310, 914, 1351, 1468
CviQI	GTAC	5 cut(s)	874, 954, 1677, 1722, 1942
DdeI	CTNAG	2 cut(s)	199, 958
DpnI	GATC	5 cut(s)	282, 659, 1233, 1553, 1686
DpnII	GATC	5 cut(s)	280, 657, 1231, 1551, 1684
DriI	GACNNNNNGTC	1 cut(s)	602
EaeI	YGGCCR	2 cut(s)	466, 828
Eam1104I	CTCTTC	4 cut(s)	73, 98, 1351, 1455
Eam1105I	GACNNNNNGTC	1 cut(s)	602
EarI	CTCTTC	4 cut(s)	73, 98, 1351, 1455
EciI	GGCGGA	1 cut(s)	628
Eco105I	TACGTA	1 cut(s)	429
Eco130I	CCWWGG	2 cut(s)	784, 1658
Eco147I	AGGCCT	1 cut(s)	634
Eco24I	GRGCYC	2 cut(s)	551, 792
Eco47I	GGWCC	5 cut(s)	824, 1382, 1472, 1655, 1772
Eco88I	CYCGRG	1 cut(s)	1680
EcoNI	CCTNNNNNAGG	1 cut(s)	797
EcoO109I	RGGNCCY	2 cut(s)	547, 789
EcoRI	GAATTC	1 cut(s)	590
EcoRII	CCWGG	3 cut(s)	463, 820, 1774
EcoT14I	CCWWGG	2 cut(s)	784, 1658
EcoT22I	ATGCAT	1 cut(s)	818
EcoT38I	GRGCYC	2 cut(s)	551, 792
ErhI	CCWWGG	2 cut(s)	784, 1658
Esp3I	CGTCTC	1 cut(s)	202
FaeI	CATG	6 cut(s)	278, 287, 313, 917, 1354, 1471
FalI	AAGNNNNNCTT	4 cut(s)	507, 539, 740, 772
FaqI	GGGAC	1 cut(s)	1841
FatI	CATG	6 cut(s)	274, 283, 309, 913, 1350, 1467
FauI	CCCGC	2 cut(s)	745, 1494
FbaI	TGATCA	3 cut(s)	280, 657, 1551
FblI	GTMKAC	1 cut(s)	1029
Fnu4HI	GCNGC	1 cut(s)	1860
FriOI	GRGCYC	2 cut(s)	551, 792
Fsp4HI	GCNGC	1 cut(s)	1860
FspBI	CTAG	3 cut(s)	60, 732, 1740
GlaI	GCGC	2 cut(s)	497, 1196
GluI	GCNGC	1 cut(s)	1860
GsaI	CCCAGC	1 cut(s)	921
HaeIII	GGCC	7 cut(s)	468, 549, 634, 790, 803, 830, 1578
HapII	CCGG	4 cut(s)	647, 827, 1681, 1987
HgaI	GACGC	1 cut(s)	915
HhaI	GCGC	2 cut(s)	498, 1197
Hin1II	CATG	6 cut(s)	278, 287, 313, 917, 1354, 1471
Hin6I	GCGC	2 cut(s)	496, 1195
HinP1I	GCGC	2 cut(s)	496, 1195
HinfI	GANTC	7 cut(s)	305, 368, 437, 520, 536, 965, 1564
HpaII	CCGG	4 cut(s)	647, 827, 1681, 1987
HphI	GGTGA	6 cut(s)	1058, 1100, 1173, 1295, 1420, 1679
Hpy166II	GTNNAC	6 cut(s)	845, 1030, 1313, 1589, 1694, 1745
Hpy8I	GTNNAC	6 cut(s)	845, 1030, 1313, 1589, 1694, 1745
Hpy99I	CGWCG	2 cut(s)	221, 611
HpyCH4III	ACNGT	6 cut(s)	708, 1034, 1153, 1447, 1761, 1922
HpyCH4IV	ACGT	2 cut(s)	428, 1842
HpyF10VI	GCNNNNNNNGC	4 cut(s)	269, 728, 1234, 1462
HpyF3I	CTNAG	2 cut(s)	199, 958
HpySE526I	ACGT	2 cut(s)	428, 1842
Hsp92II	CATG	6 cut(s)	278, 287, 313, 917, 1354, 1471
HspAI	GCGC	2 cut(s)	496, 1195
Kpn2I	TCCGGA	2 cut(s)	646, 1986
Ksp22I	TGATCA	3 cut(s)	280, 657, 1551
Kzo9I	GATC	5 cut(s)	280, 657, 1231, 1551, 1684
LguI	GCTCTTC	2 cut(s)	98, 1455
LmnI	GCTCC	3 cut(s)	23, 155, 197
Lsp1109I	GCAGC	1 cut(s)	1846
LweI	GCATC	6 cut(s)	507, 543, 803, 825, 1818, 1992
MaeI	CTAG	3 cut(s)	60, 732, 1740
MaeII	ACGT	2 cut(s)	428, 1842
MaeIII	GTNAC	4 cut(s)	245, 1210, 1256, 1969
MalI	GATC	5 cut(s)	282, 659, 1233, 1553, 1686
MbiI	CCGCTC	1 cut(s)	150
MboI	GATC	5 cut(s)	280, 657, 1231, 1551, 1684
MfeI	CAATTG	1 cut(s)	1095
MflI	RGATCY	1 cut(s)	1684
MhlI	GDGCHC	4 cut(s)	551, 792, 847, 1467
MlsI	TGGCCA	1 cut(s)	468
MluNI	TGGCCA	1 cut(s)	468
MlyI	GAGTC	2 cut(s)	362, 431
MmeI	TCCRAC	4 cut(s)	200, 1360, 1592, 1902
Mox20I	TGGCCA	1 cut(s)	468
Mph1103I	ATGCAT	1 cut(s)	818
MroI	TCCGGA	2 cut(s)	646, 1986
MroXI	GAANNNNTTC	2 cut(s)	67, 519
MscI	TGGCCA	1 cut(s)	468
MseI	TTAA	6 cut(s)	447, 741, 1071, 1398, 1604, 1839
MslI	CAYNNNNRTG	2 cut(s)	1136, 1988
Msp20I	TGGCCA	1 cut(s)	468
MspA1I	CMGCKG	1 cut(s)	1819
MspI	CCGG	4 cut(s)	647, 827, 1681, 1987
MspR9I	CCNGG	5 cut(s)	465, 822, 1681, 1682, 1776
MunI	CAATTG	1 cut(s)	1095
MvaI	CCWGG	3 cut(s)	465, 822, 1776
MvnI	CGCG	2 cut(s)	336, 1197
MwoI	GCNNNNNNNGC	4 cut(s)	269, 728, 1234, 1462
NciI	CCSGG	2 cut(s)	1681, 1682
NdeII	GATC	5 cut(s)	280, 657, 1231, 1551, 1684
NlaIII	CATG	6 cut(s)	278, 287, 313, 917, 1354, 1471
NlaIV	GGNNCC	7 cut(s)	549, 790, 791, 1146, 1428, 1455, 1473
NmuCI	GTSAC	2 cut(s)	245, 1969
NruI	TCGCGA	1 cut(s)	336
NsiI	ATGCAT	1 cut(s)	818
NspI	RCATGY	2 cut(s)	917, 1354
PaeI	GCATGC	1 cut(s)	917
PceI	AGGCCT	1 cut(s)	634
PciSI	GCTCTTC	2 cut(s)	98, 1455
PcsI	WCGNNNNNNNCGW	2 cut(s)	606, 1240
PdmI	GAANNNNTTC	2 cut(s)	67, 519
PfeI	GAWTC	5 cut(s)	305, 520, 536, 965, 1564
PflMI	CCANNNNNTGG	2 cut(s)	1190, 1574
PkrI	GCNGC	1 cut(s)	1861
PleI	GAGTC	2 cut(s)	362, 431
PpsI	GAGTC	2 cut(s)	362, 431
Ppu21I	YACGTR	1 cut(s)	429
PshBI	ATTAAT	2 cut(s)	447, 1071
PsiI	TTATAA	1 cut(s)	1791
Psp6I	CCWGG	3 cut(s)	463, 820, 1774
PspFI	CCCAGC	1 cut(s)	917
PspGI	CCWGG	3 cut(s)	463, 820, 1774
PspN4I	GGNNCC	7 cut(s)	549, 790, 791, 1146, 1428, 1455, 1473
PspOMI	GGGCCC	2 cut(s)	547, 788
PspPI	GGNCC	9 cut(s)	547, 548, 788, 789, 824, 1382, 1472, 1655, 1772
PsrI	GAACNNNNNNTAC	2 cut(s)	995, 1027
PsuI	RGATCY	1 cut(s)	1684
RruI	TCGCGA	1 cut(s)	336
RsaI	GTAC	5 cut(s)	875, 955, 1678, 1723, 1943
RsaNI	GTAC	5 cut(s)	874, 954, 1677, 1722, 1942
RseI	CAYNNNNRTG	2 cut(s)	1136, 1988
SapI	GCTCTTC	2 cut(s)	98, 1455
SaqAI	TTAA	6 cut(s)	447, 741, 1071, 1398, 1604, 1839
SatI	GCNGC	1 cut(s)	1860
Sau3AI	GATC	5 cut(s)	280, 657, 1231, 1551, 1684
Sau96I	GGNCC	9 cut(s)	547, 548, 788, 789, 824, 1382, 1472, 1655, 1772
ScaI	AGTACT	1 cut(s)	1723
SchI	GAGTC	2 cut(s)	362, 431
ScrFI	CCNGG	5 cut(s)	465, 822, 1681, 1682, 1776
SduI	GDGCHC	4 cut(s)	551, 792, 847, 1467
SfaNI	GCATC	6 cut(s)	507, 543, 803, 825, 1818, 1992
SfcI	CTRYAG	1 cut(s)	1446
SinI	GGWCC	5 cut(s)	824, 1382, 1472, 1655, 1772
SmaI	CCCGGG	1 cut(s)	1682
SmiMI	CAYNNNNRTG	2 cut(s)	1136, 1988
SmlI	CTYRAG	1 cut(s)	1250
SmoI	CTYRAG	1 cut(s)	1250
SnaBI	TACGTA	1 cut(s)	429
SphI	GCATGC	1 cut(s)	917
SseBI	AGGCCT	1 cut(s)	634
SsiI	CCGC	7 cut(s)	123, 148, 210, 639, 752, 1501, 1819
SspI	AATATT	1 cut(s)	1641
SspMI	CTAG	3 cut(s)	60, 732, 1740
StuI	AGGCCT	1 cut(s)	634
StyD4I	CCNGG	5 cut(s)	463, 820, 1679, 1680, 1774
StyI	CCWWGG	2 cut(s)	784, 1658
TaaI	ACNGT	6 cut(s)	708, 1034, 1153, 1447, 1761, 1922
TaiI	ACGT	2 cut(s)	431, 1845
TaqI	TCGA	4 cut(s)	219, 440, 492, 870
TatI	WGTACW	2 cut(s)	1721, 1941
TfiI	GAWTC	5 cut(s)	305, 520, 536, 965, 1564
Tru1I	TTAA	6 cut(s)	447, 741, 1071, 1398, 1604, 1839
Tru9I	TTAA	6 cut(s)	447, 741, 1071, 1398, 1604, 1839
TscAI	CASTG	7 cut(s)	703, 713, 852, 1039, 1158, 1450, 1701
TseFI	GTSAC	2 cut(s)	245, 1969
TseI	GCWGC	1 cut(s)	1859
Tsp45I	GTSAC	2 cut(s)	245, 1969
TspDTI	ATGAA	4 cut(s)	717, 1283, 1291, 1971
TspGWI	ACGGA	2 cut(s)	184, 1862
TspMI	CCCGGG	1 cut(s)	1680
TspRI	CASTG	7 cut(s)	703, 713, 852, 1039, 1158, 1450, 1701
Van91I	CCANNNNNTGG	2 cut(s)	1190, 1574
VneI	GTGCAC	1 cut(s)	843
VpaK11BI	GGWCC	5 cut(s)	824, 1382, 1472, 1655, 1772
VspI	ATTAAT	2 cut(s)	447, 1071
XagI	CCTNNNNNAGG	1 cut(s)	797
XapI	RAATTY	2 cut(s)	590, 1370
XceI	RCATGY	2 cut(s)	917, 1354
XcmI	CCANNNNNNNNNTGG	3 cut(s)	281, 476, 1820
XmaI	CCCGGG	1 cut(s)	1680
XmiI	GTMKAC	1 cut(s)	1029
XmnI	GAANNNNTTC	2 cut(s)	67, 519
XspI	CTAG	3 cut(s)	60, 732, 1740
ZrmI	AGTACT	1 cut(s)	1723
Zsp2I	ATGCAT	1 cut(s)	818

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.