Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007980

Physical Location & Seq

Genomic Coordinates Reverse (-)

7697314 .. 7704762

Gene Structure 7449 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1069900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2058 bp

ATGGAGATGGCAACCTGCAGCTTCCACAGCTCCTCCTTCACCCTTCCAAACCCTAACACCAACAACCCTAGAATTACTACCCTTTGCTCCAAGCAACTCTTCGGCTTCACCTTGCCTTCTTCCTCAACCGCCAAAACCACCACACTCAAACTCCACTCCCGGAACTCGAATCGCTTCTCCGCTCACCCGCCAATCAAATGCTCCGTCTCCGAAGCCACCGAAGCCGCAGCCATCGAAACAGAGAGGAGGAGTAAATTGATGAGGAGAGCTGACATAAGGAACATAGCAATCGTAGCGCATGTTGACCACGGAAAAACAACTTTGGTTGATGCAATGTTGAGGCAATCAAAGGTCTTTCGTGATAACCAATTTGTACAGGAAAGGATAATGGACTCGAATGATCTAGAGCGTGAAAGGGGAATTACTATACTAAGCAAAAATACCTCAATTACTTATAAAGACTCAAAGATTAATATAATTGATACTCCTGGACATTCTGACTTTGGAGGCGAAGTCGAACGCATCCTTAATATGGTGGAAGGCATTCTTCTAGTGGTAGATTCTGTCGAGGGCCCAATGCCACAAACAAGATTTGTTTTGAAGAAGGCTCTAGAATTTGGCCATGCAGTTGTTGTTGTTGTTAATAAGATTGATAGGCCTTCAGCTCGTCCCGATTTTGTCATCAATTCCACTTTTGAACTATTTATCGAACTAAATGCGACTGATGAACAGTGTGATTTCCAAGCAATATATGCTAGTGGTATTAAAGGAAAGGCTGGGTTGTCAGCTGATAAATTGGCAGATGATCTTGGGCCCCTTTTCGAGGCCATAATGAGATGCATACCTGGGCCTGCTATTGACAAAGATGGTGCACTTCAAATGCTTGTTACAAATATCGAGTATGATGAACATAAAGGACGAATAGCTATTGGACGTTTGCATGCTGGGGTTCTGAAGAAAGGGATGGATGTGAAGGTATGCACCTCAGAAGATGCATGTCGAGTTGCAAGAGTTAGTGAGCTTTTTGTGTATGAGAAATTTAGTAGGGTTCCTGCAGAAAGTGTGGAGGCTGGTGATATATGTGCTGTTTGTGGAATCAATGATATTCAGATTGGCGAGACAATTGCTTGTAAGATCGCTGGGACACCATTACCTGCCATTAGGGTTGAGGAACCAACAGTGAAAATGGCTTTTGCCATAAACACTTCACCGTTTGTTGGACGTGAGGGAAAGTATGTTACCAGTAGGAACTTACGCGATCGTCTTTCTCGTGAGCTTGAGCGTAACTTGGCTATGAAGGTTGAAGATGGCGAAACAGCAGACACCTTCATTGTTAGTGGACGGGGTACTTTACATATTACGATATTGATCGAGAACATGCGAAGAGAAGGATATGAATTCATGGTGGGACCTCCCAAAGTTATTAACAAAAGGGTGAATGACAAATTGCTGGAACCATATGAGATCGCCACTGTGGAGGTGCCAGAAGAACACATGGGCCCTGTTGTTGAGCTTCTTGGCAAAAGGAGGGGGCAGATGTTTGATATGCAAGGAGTTGGGTCTGAAGGCACAACCTTCCTCAAATATAAAATTCCTACTCGTGGCCTTCTTGGTTTGCGAAATGCAATTTTAACAGCTTCTCGTGGCACTGCAATTCTCAACACAATATTTGATAGCTACGGACCTTGGGCTGGTGATATGAGTACCCGGGATCAGGGTTCACTGGTTGCCTTTGAGGGTGGAACGAGTACTTCTTATGCCATTTCTAGTTCACAGGAGAGGGGGCAGTTGTTTATTGGTCCCGGGGTGGAAGTTTATAAAGGTCAGATAGTCGGCATCCATCAGCGGCCTGGGGACTTGTCCCTTAATGTGTGTAAGAAGAAGGCAGCAACAAATATCCGTTCCAACAAAGAACAAACTGTGATTCTTGACACCCCATTGGATTACAGTCTGGATGACTGCATTGAGTATATTCAAGAGGATGAATTGGTGGAGGTAACTCCTGCGAGTATCCGAATGTGCAAGAACCCAAAGTTTTCAAAGAAATCAAACAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0031647 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050821 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

686

Amino Acids

75.58

Weight (kDa)

6.67

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	91 - 282	7.9e-53	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	293 - 375	1.3e-08	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	306 - 376	3e-12	Elongation factor Tu domain 2
EFG_C	PF00679	482 - 568	1.4e-20	Elongation factor G C-terminus
BipA_C	PF21018	572 - 676	7.9e-39	TypA/BipA C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13650 AT5G13650
fragaria_vesca	FvH4_6g23530 FvH4_6g23530 FvH4_6g23530 FvH4_6g23530
malus_domestica	MD07G1028800.v1.1 MD12G1072500.v1.1 MD14G1069900.v1.1
prunus_persica	Prupe.7G064400_v2.0.a1
pyrus_communis	pycom12g06100 pycom12g06110 pycom14g05790
rosa_chinensis	RchiOBHm_Chr3g0481531
rosa_laevigata	RLG00000023388
rosa_multiflora	Rmu_sc0010846.1_g000003 Rmu_sc0040423.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400150 Rroxscaffold_6G00400350
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243700 Rh3BG278800 Rh3DG270600
rosa_wichuraiana	Rw3G021770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	456, 1818
Acc36I	ACCTGC	2 cut(s)	23, 1162
AccB1I	GGYRCC	1 cut(s)	1480
AccBSI	CCGCTC	1 cut(s)	182
AccII	CGCG	1 cut(s)	1257
AciI	CCGC	5 cut(s)	129, 180, 188, 225, 1846
AclWI	GGATC	1 cut(s)	1719
AcoI	YGGCCR	1 cut(s)	619
AcsI	RAATTY	4 cut(s)	614, 1037, 1397, 1590
AcuI	CTGAAG	3 cut(s)	645, 974, 1584
AfaI	GTAC	4 cut(s)	375, 1348, 1705, 1750
AfiI	CCNNNNNNNGG	6 cut(s)	532, 823, 1217, 1601, 1691, 1714
AgsI	TTSAA	6 cut(s)	601, 698, 878, 1304, 1976, 2040
AjiI	CACGTC	1 cut(s)	1223
AjnI	CCWGG	3 cut(s)	487, 844, 1849
AjuI	GAANNNNNNNTTGG	2 cut(s)	83, 115
Alw21I	GWGCWC	1 cut(s)	874
Alw26I	GTCTC	2 cut(s)	211, 1112
Alw44I	GTGCAC	1 cut(s)	870
AlwI	GGATC	1 cut(s)	1719
Ama87I	CYCGRG	2 cut(s)	1707, 1802
AoxI	GGCC	9 cut(s)	571, 619, 656, 812, 825, 848, 1498, 1603, 1847
ApaI	GGGCCC	3 cut(s)	575, 816, 1502
ApaLI	GTGCAC	1 cut(s)	870
ApeKI	GCWGC	3 cut(s)	18, 227, 1886
ApoI	RAATTY	4 cut(s)	614, 1037, 1397, 1590
ArsI	GACNNNNNNTTYG	2 cut(s)	189, 221
AseI	ATTAAT	1 cut(s)	471
Asp700I	GAANNNNTTC	2 cut(s)	173, 543
AspLEI	GCGC	1 cut(s)	298
AsuC2I	CCSGG	5 cut(s)	160, 1708, 1709, 1803, 1804
AsuHPI	GGTGA	7 cut(s)	31, 100, 176, 1085, 1200, 1447, 1706
AvaI	CYCGRG	2 cut(s)	1707, 1802
AvaII	GGWCC	3 cut(s)	1409, 1682, 1799
BaeGI	GKGCMC	4 cut(s)	575, 816, 874, 1502
BalI	TGGCCA	1 cut(s)	621
BanI	GGYRCC	1 cut(s)	1480
BanII	GRGCYC	3 cut(s)	575, 816, 1502
BauI	CACGAG	3 cut(s)	1269, 1599, 1641
Bbv12I	GWGCWC	1 cut(s)	874
BbvI	GCAGC	3 cut(s)	30, 239, 1898
BccI	CCATC	5 cut(s)	239, 860, 958, 1301, 1848
BcgI	CGANNNNNNTGC	4 cut(s)	698, 732, 1106, 1140
BciT130I	CCWGG	3 cut(s)	489, 846, 1851
BciVI	GTATCC	1 cut(s)	2021
BcnI	CCSGG	5 cut(s)	160, 1708, 1709, 1803, 1804
BcoDI	GTCTC	2 cut(s)	211, 1112
BfaI	CTAG	6 cut(s)	69, 404, 551, 611, 756, 1767
BfmI	CTRYAG	2 cut(s)	16, 1053
BfuAI	ACCTGC	2 cut(s)	23, 1162
BfuI	GTATCC	1 cut(s)	2021
BisI	GCNGC	5 cut(s)	19, 225, 228, 1847, 1887
BlsI	GCNGC	5 cut(s)	20, 226, 229, 1848, 1888
BmcAI	AGTACT	1 cut(s)	1750
Bme1390I	CCNGG	8 cut(s)	160, 489, 846, 1708, 1709, 1803, 1804, 1851
Bme18I	GGWCC	3 cut(s)	1409, 1682, 1799
BmeT110I	CYCGRG	2 cut(s)	1707, 1802
BmgBI	CACGTC	1 cut(s)	1223
BmrFI	CCNGG	8 cut(s)	160, 489, 846, 1708, 1709, 1803, 1804, 1851
BmsI	GCATC	5 cut(s)	319, 531, 827, 982, 1845
BpuEI	CTTGAG	1 cut(s)	1298
BpuMI	CCSGG	5 cut(s)	160, 1708, 1709, 1803, 1804
BsaBI	GATNNNNATC	1 cut(s)	1367
BsaJI	CCNNGG	7 cut(s)	307, 845, 1685, 1707, 1802, 1803, 1850
BsaXI	ACNNNNNCTCC	6 cut(s)	17, 47, 135, 165, 498, 528
Bsc4I	CCNNNNNNNGG	6 cut(s)	532, 823, 1217, 1601, 1691, 1714
Bse1I	ACTGG	2 cut(s)	1242, 1728
Bse3DI	GCAATG	1 cut(s)	339
Bse8I	GATNNNNATC	1 cut(s)	1367
BseBI	CCWGG	3 cut(s)	489, 846, 1851
BseDI	CCNNGG	7 cut(s)	307, 845, 1685, 1707, 1802, 1803, 1850
BseGI	GGATG	6 cut(s)	522, 969, 973, 1836, 1960, 1987
BseJI	GATNNNNATC	1 cut(s)	1367
BseLI	CCNNNNNNNGG	6 cut(s)	532, 823, 1217, 1601, 1691, 1714
BseMI	GCAATG	1 cut(s)	339
BseMII	CTCAG	1 cut(s)	999
BseNI	ACTGG	2 cut(s)	1242, 1728
BseRI	GAGGAG	4 cut(s)	22, 259, 262, 277
BseSI	GKGCMC	4 cut(s)	575, 816, 874, 1502
BseXI	GCAGC	3 cut(s)	30, 239, 1898
BseYI	CCCAGC	3 cut(s)	776, 944, 1139
Bsh1236I	CGCG	1 cut(s)	1257
Bsh1285I	CGRYCG	1 cut(s)	1261
BshFI	GGCC	9 cut(s)	573, 621, 658, 814, 827, 850, 1500, 1605, 1849
BshNI	GGYRCC	1 cut(s)	1480
BsiEI	CGRYCG	1 cut(s)	1261
BsiHKAI	GWGCWC	1 cut(s)	874
BsiHKCI	CYCGRG	2 cut(s)	1707, 1802
BsiSI	CCGG	3 cut(s)	160, 1708, 1803
BslFI	GGGAC	6 cut(s)	654, 1156, 1422, 1785, 1846, 1868
BslI	CCNNNNNNNGG	6 cut(s)	532, 823, 1217, 1601, 1691, 1714
BsmAI	GTCTC	2 cut(s)	211, 1112
BsmBI	CGTCTC	1 cut(s)	211
BsmFI	GGGAC	6 cut(s)	654, 1156, 1422, 1785, 1846, 1868
BsmI	GAATGC	1 cut(s)	543
BsnI	GGCC	9 cut(s)	573, 621, 658, 814, 827, 850, 1500, 1605, 1849
BsoBI	CYCGRG	2 cut(s)	1707, 1802
Bsp120I	GGGCCC	3 cut(s)	571, 812, 1498
Bsp1286I	GDGCHC	4 cut(s)	575, 816, 874, 1502
Bsp1407I	TGTACA	1 cut(s)	373
Bsp143I	GATC	7 cut(s)	400, 805, 1134, 1258, 1368, 1464, 1711
BspACI	CCGC	5 cut(s)	129, 180, 188, 225, 1846
BspANI	GGCC	9 cut(s)	573, 621, 658, 814, 827, 850, 1500, 1605, 1849
BspCNI	CTCAG	1 cut(s)	998
BspFNI	CGCG	1 cut(s)	1257
BspMAI	CTGCAG	2 cut(s)	20, 1057
BspMI	ACCTGC	2 cut(s)	23, 1162
BspPI	GGATC	1 cut(s)	1719
BspT107I	GGYRCC	1 cut(s)	1480
BsrBI	CCGCTC	1 cut(s)	182
BsrDI	GCAATG	1 cut(s)	339
BsrGI	TGTACA	1 cut(s)	373
BsrI	ACTGG	2 cut(s)	1242, 1728
BssECI	CCNNGG	7 cut(s)	307, 845, 1685, 1707, 1802, 1803, 1850
BssMI	GATC	7 cut(s)	400, 805, 1134, 1258, 1368, 1464, 1711
BssSI	CACGAG	3 cut(s)	1269, 1599, 1641
BssT1I	CCWWGG	1 cut(s)	1685
Bst2BI	CACGAG	3 cut(s)	1269, 1599, 1641
Bst2UI	CCWGG	3 cut(s)	489, 846, 1851
Bst4CI	ACNGT	6 cut(s)	732, 1180, 1212, 1474, 1921, 1949
Bst6I	CTCTTC	2 cut(s)	104, 1378
BstAPI	GCANNNNNTGC	1 cut(s)	752
BstAUI	TGTACA	1 cut(s)	373
BstC8I	GCNNGC	2 cut(s)	852, 942
BstDEI	CTNAG	2 cut(s)	431, 985
BstDSI	CCRYGG	1 cut(s)	307
BstENI	CCTNNNNNAGG	1 cut(s)	821
BstF5I	GGATG	6 cut(s)	522, 969, 973, 1836, 1960, 1987
BstFNI	CGCG	1 cut(s)	1257
BstHHI	GCGC	1 cut(s)	298
BstKTI	GATC	7 cut(s)	403, 808, 1137, 1261, 1371, 1467, 1714
BstMAI	GTCTC	2 cut(s)	211, 1112
BstMBI	GATC	7 cut(s)	400, 805, 1134, 1258, 1368, 1464, 1711
BstMCI	CGRYCG	1 cut(s)	1261
BstMWI	GCNNNNNNNGC	4 cut(s)	27, 221, 293, 752
BstNI	CCWGG	3 cut(s)	489, 846, 1851
BstNSI	RCATGY	4 cut(s)	302, 944, 999, 1381
BstSCI	CCNGG	8 cut(s)	158, 487, 844, 1706, 1707, 1801, 1802, 1849
BstSFI	CTRYAG	2 cut(s)	16, 1053
BstSLI	GKGCMC	4 cut(s)	575, 816, 874, 1502
BstUI	CGCG	1 cut(s)	1257
BstV1I	GCAGC	3 cut(s)	30, 239, 1898
BsuI	GTATCC	1 cut(s)	2021
BsuRI	GGCC	9 cut(s)	573, 621, 658, 814, 827, 850, 1500, 1605, 1849
BtgI	CCRYGG	1 cut(s)	307
BtrI	CACGTC	1 cut(s)	1223
BtsCI	GGATG	6 cut(s)	522, 969, 973, 1836, 1960, 1987
BtsI	GCAGTG	1 cut(s)	1647
BtsIMutI	CAGTG	5 cut(s)	737, 1185, 1470, 1647, 1721
BveI	ACCTGC	2 cut(s)	23, 1162
Cac8I	GCNNGC	2 cut(s)	852, 942
CfoI	GCGC	1 cut(s)	298
Cfr9I	CCCGGG	2 cut(s)	1707, 1802
Csp6I	GTAC	4 cut(s)	374, 1347, 1704, 1749
CviAII	CATG	7 cut(s)	299, 623, 941, 996, 1378, 1402, 1495
CviQI	GTAC	4 cut(s)	374, 1347, 1704, 1749
DdeI	CTNAG	2 cut(s)	431, 985
DpnI	GATC	7 cut(s)	402, 807, 1136, 1260, 1370, 1466, 1713
DpnII	GATC	7 cut(s)	400, 805, 1134, 1258, 1368, 1464, 1711
EaeI	YGGCCR	1 cut(s)	619
Eam1104I	CTCTTC	2 cut(s)	104, 1378
EarI	CTCTTC	2 cut(s)	104, 1378
Eco130I	CCWWGG	1 cut(s)	1685
Eco147I	AGGCCT	1 cut(s)	658
Eco24I	GRGCYC	3 cut(s)	575, 816, 1502
Eco47I	GGWCC	3 cut(s)	1409, 1682, 1799
Eco57I	CTGAAG	3 cut(s)	645, 974, 1584
Eco88I	CYCGRG	2 cut(s)	1707, 1802
EcoNI	CCTNNNNNAGG	1 cut(s)	821
EcoO109I	RGGNCCY	4 cut(s)	571, 813, 1409, 1499
EcoRI	GAATTC	1 cut(s)	1397
EcoRII	CCWGG	3 cut(s)	487, 844, 1849
EcoT14I	CCWWGG	1 cut(s)	1685
EcoT22I	ATGCAT	2 cut(s)	842, 997
EcoT38I	GRGCYC	3 cut(s)	575, 816, 1502
ErhI	CCWWGG	1 cut(s)	1685
Esp3I	CGTCTC	1 cut(s)	211
FaeI	CATG	7 cut(s)	302, 626, 944, 999, 1381, 1405, 1498
FalI	AAGNNNNNCTT	4 cut(s)	83, 115, 531, 563
FaqI	GGGAC	6 cut(s)	654, 1156, 1422, 1785, 1846, 1868
FatI	CATG	7 cut(s)	298, 622, 940, 995, 1377, 1401, 1494
FauI	CCCGC	1 cut(s)	195
FauNDI	CATATG	1 cut(s)	1459
Fnu4HI	GCNGC	5 cut(s)	19, 225, 228, 1847, 1887
FokI	GGATG	6 cut(s)	509, 976, 980, 1823, 1967, 1994
FriOI	GRGCYC	3 cut(s)	575, 816, 1502
Fsp4HI	GCNGC	5 cut(s)	19, 225, 228, 1847, 1887
FspBI	CTAG	6 cut(s)	69, 404, 551, 611, 756, 1767
GlaI	GCGC	1 cut(s)	297
GluI	GCNGC	5 cut(s)	19, 225, 228, 1847, 1887
GsaI	CCCAGC	3 cut(s)	780, 948, 1143
HaeIII	GGCC	9 cut(s)	573, 621, 658, 814, 827, 850, 1500, 1605, 1849
HapII	CCGG	3 cut(s)	160, 1708, 1803
HhaI	GCGC	1 cut(s)	298
Hin1II	CATG	7 cut(s)	302, 626, 944, 999, 1381, 1405, 1498
Hin6I	GCGC	1 cut(s)	296
HinP1I	GCGC	1 cut(s)	296
HincII	GTYRAC	1 cut(s)	304
HindII	GTYRAC	1 cut(s)	304
HinfI	GANTC	6 cut(s)	169, 392, 461, 560, 1095, 1924
HpaII	CCGG	3 cut(s)	160, 1708, 1803
HphI	GGTGA	7 cut(s)	31, 100, 176, 1085, 1200, 1447, 1706
Hpy166II	GTNNAC	5 cut(s)	304, 872, 1340, 1721, 1772
Hpy188I	TCNGA	8 cut(s)	211, 499, 954, 988, 1110, 1564, 1827, 2015
Hpy188III	TCNNGA	9 cut(s)	359, 404, 611, 671, 1271, 1372, 1928, 1952, 1976
Hpy8I	GTNNAC	5 cut(s)	304, 872, 1340, 1721, 1772
HpyCH4III	ACNGT	6 cut(s)	732, 1180, 1212, 1474, 1921, 1949
HpyCH4IV	ACGT	2 cut(s)	934, 1222
HpyF10VI	GCNNNNNNNGC	4 cut(s)	27, 221, 293, 752
HpyF3I	CTNAG	2 cut(s)	431, 985
HpySE526I	ACGT	2 cut(s)	934, 1222
Hsp92II	CATG	7 cut(s)	302, 626, 944, 999, 1381, 1405, 1498
HspAI	GCGC	1 cut(s)	296
Kzo9I	GATC	7 cut(s)	400, 805, 1134, 1258, 1368, 1464, 1711
LmnI	GCTCC	3 cut(s)	35, 92, 206
Lsp1109I	GCAGC	3 cut(s)	30, 239, 1898
LweI	GCATC	5 cut(s)	319, 531, 827, 982, 1845
MaeI	CTAG	6 cut(s)	69, 404, 551, 611, 756, 1767
MaeII	ACGT	2 cut(s)	934, 1222
MaeIII	GTNAC	4 cut(s)	886, 1237, 1283, 1996
MalI	GATC	7 cut(s)	402, 807, 1136, 1260, 1370, 1466, 1713
MbiI	CCGCTC	1 cut(s)	182
MboI	GATC	7 cut(s)	400, 805, 1134, 1258, 1368, 1464, 1711
MfeI	CAATTG	1 cut(s)	1122
MhlI	GDGCHC	4 cut(s)	575, 816, 874, 1502
MlsI	TGGCCA	1 cut(s)	621
MluNI	TGGCCA	1 cut(s)	621
MlyI	GAGTC	2 cut(s)	386, 455
MmeI	TCCRAC	2 cut(s)	1198, 1929
Mox20I	TGGCCA	1 cut(s)	621
Mph1103I	ATGCAT	2 cut(s)	842, 997
MroXI	GAANNNNTTC	2 cut(s)	173, 543
MscI	TGGCCA	1 cut(s)	621
MseI	TTAA	7 cut(s)	471, 528, 642, 765, 1425, 1631, 1866
Msp20I	TGGCCA	1 cut(s)	621
MspA1I	CMGCKG	2 cut(s)	788, 1846
MspI	CCGG	3 cut(s)	160, 1708, 1803
MspR9I	CCNGG	8 cut(s)	160, 489, 846, 1708, 1709, 1803, 1804, 1851
MunI	CAATTG	1 cut(s)	1122
Mva1269I	GAATGC	1 cut(s)	543
MvaI	CCWGG	3 cut(s)	489, 846, 1851
MvnI	CGCG	1 cut(s)	1257
MwoI	GCNNNNNNNGC	4 cut(s)	27, 221, 293, 752
NciI	CCSGG	5 cut(s)	160, 1708, 1709, 1803, 1804
NdeI	CATATG	1 cut(s)	1459
NdeII	GATC	7 cut(s)	400, 805, 1134, 1258, 1368, 1464, 1711
NlaIII	CATG	7 cut(s)	302, 626, 944, 999, 1381, 1405, 1498
NsiI	ATGCAT	2 cut(s)	842, 997
NspI	RCATGY	4 cut(s)	302, 944, 999, 1381
PaeI	GCATGC	1 cut(s)	944
PceI	AGGCCT	1 cut(s)	658
PcsI	WCGNNNNNNNCGW	1 cut(s)	1267
PctI	GAATGC	1 cut(s)	543
PdmI	GAANNNNTTC	2 cut(s)	173, 543
PfeI	GAWTC	4 cut(s)	169, 560, 1095, 1924
PfoI	TCCNGGA	2 cut(s)	158, 487
PkrI	GCNGC	5 cut(s)	20, 226, 229, 1848, 1888
Ple19I	CGATCG	1 cut(s)	1261
PleI	GAGTC	2 cut(s)	386, 455
PpsI	GAGTC	2 cut(s)	386, 455
PpuMI	RGGWCCY	1 cut(s)	1409
PshBI	ATTAAT	1 cut(s)	471
PsiI	TTATAA	2 cut(s)	456, 1818
Psp5II	RGGWCCY	1 cut(s)	1409
Psp6I	CCWGG	3 cut(s)	487, 844, 1849
PspFI	CCCAGC	3 cut(s)	776, 944, 1139
PspGI	CCWGG	3 cut(s)	487, 844, 1849
PspOMI	GGGCCC	3 cut(s)	571, 812, 1498
PspPPI	RGGWCCY	1 cut(s)	1409
PstI	CTGCAG	2 cut(s)	20, 1057
PvuI	CGATCG	1 cut(s)	1261
PvuII	CAGCTG	1 cut(s)	788
RsaI	GTAC	4 cut(s)	375, 1348, 1705, 1750
RsaNI	GTAC	4 cut(s)	374, 1347, 1704, 1749
SaqAI	TTAA	7 cut(s)	471, 528, 642, 765, 1425, 1631, 1866
SatI	GCNGC	5 cut(s)	19, 225, 228, 1847, 1887
Sau3AI	GATC	7 cut(s)	400, 805, 1134, 1258, 1368, 1464, 1711
ScaI	AGTACT	1 cut(s)	1750
SchI	GAGTC	2 cut(s)	386, 455
ScrFI	CCNGG	8 cut(s)	160, 489, 846, 1708, 1709, 1803, 1804, 1851
SduI	GDGCHC	4 cut(s)	575, 816, 874, 1502
SfaNI	GCATC	5 cut(s)	319, 531, 827, 982, 1845
SfcI	CTRYAG	2 cut(s)	16, 1053
SinI	GGWCC	3 cut(s)	1409, 1682, 1799
SmaI	CCCGGG	2 cut(s)	1709, 1804
SmlI	CTYRAG	1 cut(s)	1277
SmoI	CTYRAG	1 cut(s)	1277
SphI	GCATGC	1 cut(s)	944
SseBI	AGGCCT	1 cut(s)	658
SsiI	CCGC	5 cut(s)	129, 180, 188, 225, 1846
SspI	AATATT	1 cut(s)	1668
SspMI	CTAG	6 cut(s)	69, 404, 551, 611, 756, 1767
StuI	AGGCCT	1 cut(s)	658
StyD4I	CCNGG	8 cut(s)	158, 487, 844, 1706, 1707, 1801, 1802, 1849
StyI	CCWWGG	1 cut(s)	1685
TaaI	ACNGT	6 cut(s)	732, 1180, 1212, 1474, 1921, 1949
TaiI	ACGT	2 cut(s)	937, 1225
TatI	WGTACW	2 cut(s)	373, 1748
TauI	GCSGC	2 cut(s)	227, 1849
TfiI	GAWTC	4 cut(s)	169, 560, 1095, 1924
Tru1I	TTAA	7 cut(s)	471, 528, 642, 765, 1425, 1631, 1866
Tru9I	TTAA	7 cut(s)	471, 528, 642, 765, 1425, 1631, 1866
TscAI	CASTG	5 cut(s)	737, 1185, 1477, 1654, 1728
TseI	GCWGC	3 cut(s)	18, 227, 1886
TspDTI	ATGAA	7 cut(s)	741, 921, 1310, 1318, 1390, 1410, 1998
TspGWI	ACGGA	4 cut(s)	193, 324, 1695, 1889
TspMI	CCCGGG	2 cut(s)	1707, 1802
TspRI	CASTG	5 cut(s)	737, 1185, 1477, 1654, 1728
VneI	GTGCAC	1 cut(s)	870
VpaK11BI	GGWCC	3 cut(s)	1409, 1682, 1799
VspI	ATTAAT	1 cut(s)	471
XagI	CCTNNNNNAGG	1 cut(s)	821
XapI	RAATTY	4 cut(s)	614, 1037, 1397, 1590
XbaI	TCTAGA	2 cut(s)	403, 610
XceI	RCATGY	4 cut(s)	302, 944, 999, 1381
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1847
XmaI	CCCGGG	2 cut(s)	1707, 1802
XmnI	GAANNNNTTC	2 cut(s)	173, 543
XspI	CTAG	6 cut(s)	69, 404, 551, 611, 756, 1767
ZrmI	AGTACT	1 cut(s)	1750
Zsp2I	ATGCAT	2 cut(s)	842, 997

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.