FvH4_6g24100

ABC transporter A family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005424
Physical Location & Seq
Forward (+)
18136305 .. 18159411
23107 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24100.t2

Sequence Viewer

Length: 5352 bp
ATGGCTACTCGGTTATGTTTTGATGAGATCAAGCTGTTAGATTCTATTACAGTCAAATTATCAAATTTAGTTGAATTACTGAATAAACTCATCTTTCTAATTAGGAATTGCTCGAACCCAAAAATCAAAGGAGCAGTAGTATTTCATGATCAAGGTCCTCAACGTTTTGATTATAGCATTCGTCTTAATCACACATGGGCTTTCTCGGGATTTCCTGATGTTAAATCGATCATGGACACAAATGGCCCTTACTTCAATGACTTGGAACTGGGCGTGAATACTGTACCGACAATGCAATACAGCTTCAGTGGATTTTTAACTCTCCAGCAAGCACTGGATTCTTTCATAATTTTTGTGGCCCAGCAATCTGATACCAAAAATATAGAACTTCCTACTCCGCTGTCCTCTAGCACACTTTCGTCTCTCAAAGTTCCATGGACGCAATATGGTCCGTCAACCATTCGTGTAGCCCCGTTTCCAACACGTGAATACACTGATGATGAGTTCCAGTCCATCATAAAAAGTGTCATGGGTGTATTGTATCTTTTGGGATTTCTCTATCCAATTTCACGCTTGATCAGCTATTCTGTGTTTGAGAAGGAACAAAAGATAAGAGAAGGTCTCTATATGATGGGGCTGAAAGATGGGGTATTCCATCTATCATGGTTTATTGCATATGCTCTGCAGTTTGCGGTTTCTTCTCTTATTATTACAGTTTGCACGATGGATAACCTTTTCAAGTATAGTGACAAGTCTGTGGTGTTTGTTTACTTCTTCTTTTTCGGACTAAGCGCGATTATGCTGTCATTTTTAATCTCTACGTTCTTTGAACGAGCGAAGACAGCTGTTGCAGTTGGGACCCTTGCTTTCTTGGGTGCTTTTTTCCCTTACTACTCTGTCAATGACGAGGCTGTTCCTATGATTCTAAAGGTCATTGCCTCACTGCTTTCGCCTACAGCCTTTGCACTGGGTTCAATCAACTTTGCGGATTATGAGCGTGCTCATGTTGGTCTTCGTTGGAGCAATATATGGCGGGCATCGTCAGGAGTTAATTTTTCAGTCTGTCTCCTGATGATGTTGCTCGATGCGTTACTCTACTGTGTAATTGGTCTTTACCTAGATAAGGTACTTCCTAGAGAGAATGGAGTTAGGTATCCCTGGAACTTCATTTTTCAGAAGTGTTTTTGGAAGACTCCAAATGTTAACAACTACCACAATTCCAGTCCAGAAGTTCACATTCGCGACAAGGTATCCCAGAAGGCAATGTTTTCTGGTAAAGAAAATGCTAAGGCTGCTGTGGAAGCAATAACCTTTGATATGAAACAACAAGAGCTTGACCATAGATGCATCCAAATAAGGAATCTGCGTAAAGTTTATGCTAATAAAAAGGGGAAATGTTGTGCTGTCAACTCATTACAGCTCACCATGTATGAAAATCAGATTCTTGCTCTTCTAGGACATAATGGAGCTGGTAAAAGCACAACGATATCAATGCTTGTTGGCCTTCTTCGCCCTACTTCAGGGGATGCTATGGTATTTGGAAAAAATATTACAACAGACATGGAGGAGATACGGAAAGAGTTGGGTGTATGCCCTCAGCATGATATCCTTTTTCCAGAATTGACAGTGAAGGAGCACTTGGAAATATTCGCCATCTTGAAAGGTGTGCGAGAAGATTTTGTGAACAGTGTCGTGATTGATATGGTTGACCAAGTAGGGTTGGCCGATAAAATCAACACTACTGTTATGGCTCTTTCTGGAGGCATGAAGAGGAAGTTGTCTCTTGGAATTGCACTAATAGGAAATAGTAAGGTTATAATTCTTGATGAACCTACTAGTGGAATGGATCCATACTCTATGCGCTTGACTTGGCAATTAATAAAAAAGATTAGAAAGGGAAGGATAGTATTACTGACAACGCACTCAATGGATGAAGCTGAGGCACTTGGAGATCGCATAGCCATCATGGCTAATGGTTCGTTGAAATGCTGCGGAAGTTCGCTTTTCTTGAAGCATCAGTATGGGGTTGGTTATACTCTAACTTTAGTGAAGTCTGCACCCACTGCCTCTATGGCTGCTGATATTGTTTACCGCCATATCCCATCAGCAACATGTGTGAGTGAGGTTGGAACTGAGATTTCCTTTAAGCTTCCTTTGGCATCTTCAACATCCTTCGAAAGTATGTTTAGAGAAATTGAGAGTTGCATGAGATCATCCATATTAAACTTAGGAACAAGTTCTGATGAGAAGGACTATATTGGCATTGAAAGTTATGGCATATCTGTCACAACTTTGGAGGAGGTGTTCCTGAGGGTTGCAGGCTGTGATTATGACGAAGCTGCTTCTTTTGACCTGAAGAATGGTTTACTGTGCCCTGAGTCTCAGATCTCTCAGACTTCTCATGATCCTACCCACAAACAGATTTTTCACTCAAAGAAATCTTTTGCTTATTATAAAGGGATTCTTGGGGTCTTATTTGAAATGGTGGGAAGAGCCTGTGGTTTGATTTTTTCTACAGTGTTAAGTTTCTTAAACTTCTTAGGCGTGCAATGCTGTGGCTGTTGTATAATTTCAAGGTCGACATTTTGGAGGCATTCTAAAGCATTATTCATAAAGAGGGCAATTTCTGCCCGTAGAGACCGTAAAACAATCGTGTTCCAGCTTGTAATTCCTGCAGTTTTCTTATTTTTCGGTCTTTTGTTTCTCAAGCTCAAGCCACATCCTGATCAGGAGTCTGTTACCTTCACAACTTCACATTTTAATCCACTATTACGTGGTGGAGGTGGTGGTGGTCCAATTCCTTATGATCTGTCCTGGCCTATTGCACATGAGGTTGCAGAACACATAACAGGAGGTTGGATACAAACCTTCAAACCAAGTGGGTATAAATTTCCCAACTCAGAGAAGGCATTGAATGATGCAATTGAGGCAGCAGGGGAAACTCTGGGACCTGCTTTACTTTCAATGAGTGAATTTTTGATGTCCAGTTTTAATGAATCTTACCAGTCAAGGTATGGGGCAGTTGTGATGGACAATCAGAGTGATGATGGGAGTTTAGGGTACACTGTGCTACACAACAGTTCCTGTCAGCATGCTGCCCCAACCTTTATCAATTTGGTGAATGCTGCAATTCTTAGGCTTGCTTCTCGTGATAAGAATATGACAATTCAAACACGCAATCATCCTCTGCCAATGACACAAAGTCAACATCTTCAACGGCATGATTTGGATGCTTTCTCTGCTGCAGTTATTGTTAGTATCGCCTTCTCCTTTATTCCTGCATCGTTTGCTGTTCCTATAGTGAAGGAACGTGAAGTAAAAGCTAAGCACCAACAACTTATTAGTGGGGTTTCCATACTGTCATATTGGACTTCTACATACATATGGGACTTCATTAGCTTCCTCTTTCCTTCATCGTTTGCCATAATTCTTTTTTACATATTTGGTTTGGACCAGTTTATTGGAAGGGGATGTTTACTGTCGACAGTCATCATGTTTTTAGCATACGGATTAGCAATCGCATCATCAACATACTGCCTTACATTTTTCTTTTCTGATCACACTATGGCTCAGAATGTGGTTCTCTTAGTCCACTTTTTCACTGGACTCATTCTCATGGTTATCTCATTCATTATGGGGCTTATAAAGACGACAGCAAGTGCAAATTCTTTTCTTAAGAACTTTTTCAGATTATCTCCAGGATTTTGCTTTGCTGATGGACTGGCTTCACTGGCTCTTCTACGTCAAGATATGAAAAATAAATCAAGCAATAAGGCCTTTGATTGGAATGTTACTGGTGGCTCAATCTGCTATCTCGGTATTGAGAGCCTTTGCTACTTCCTTTTGGCACTTGGTCTTGAAATCTTTCCTTTCAATAAGTTGACACTAGCCACACTCAAGGAGTGGTGGAAGAGTATAAAAATTATCCACCCTGGCACTTCAAGTTATCGAGAGCCCCTTCTGACTTCTTCCGCAGAATCCATCACTCTGGACCTTGATGAGGACACAGATGTGAAGACAGAAAGAACTAGGGTACTTTCAGGTTCCATTGACAATGCCATCATCTACTTGTGTAATCTTCGTAAGGTATATCCAGGAGGACAGCAGCATGCTACAAAGGTAGCGGTACATTCATTGACTTTTTCAGTTCAAGAAGGAGAATGTTTTGGGTTTTTAGGAACAAATGGAGCTGGGAAGACCACAACTCTTTCTATGTTGACTGGTGAGGAATCTCCAACGGATGGGACTGCTTGTATATTTGGCAAGGATATTTGCTCCAATCCCAAAGCTGCACGTCAACATATTGGGTTTTGCCCACAGTTTGATGCTCTGTTAGAATATTTAACTGTTCAAGAGCATCTTGAGCTTTATGCCACGATAAAAGGAGTACCGGATTATAAAATAGATGAGGTTGTAATGGAAAAGTTGATGGAGTTTGACTTGTTAAAGCATGCCAGCAAACCATCATTTTCCCTCAGCGGGGGAAACAAGCGTAAATTATCTGTTGCAATTGCCATGATTGGAGATCCTCCTATAGTTATTCTTGATGAGCCATCAACAGGTATGGATCCAATTGCCAAAAGATTCATGTGGGAAGTGATATCTCGTTTGTCAACCAGACGAGGAAAGACGGCAGTGATACTCACTACCCATAGTATGAATGAAGCTCAAGCTCTGTGCACGCGCATCGGAATCATGGTTGGAGGCCAGTTAAGGTGCATTGGAAGTCCCCAGCATTTGAAAAATCGATTTGGGAATCATCTTGAGCTAGAGGTGAAACCTATTGAAGTTAGCTCTGTGGACTTGGACAAGCTTTGCCGTGTTATCCAAGAAAGACTCTCATATGTTCCTTCTCATCCAAGAAGCTTACTTGATGGCCTTGAAGTCTGCATTGGTGCCACTGACTCCATCGTGGCGGAAAATGCATCGGTGGCAGAGATTAGTTTATCACGAGAAATGATTATTATGATTGGACGGTGGCTTGGGAATGAAGAAAGAATAAAGCCGCTTATATCTGCCACACCACTTTCAGACGGGGTGCTTGGTGAACAGTTATTTGAGCAGTTAGATCGAGATGGTGGAATACCATTGCTAATATTTTCAGAATGGTGGCTGTCCAGTGAAAAGTTTTCAGCAATTGATTCATTTGTCCTATCATCATTTCCTGGTGCAATGTTCCAGGGAATGAATGGCTTGAGTGTTAAATATCAGTTGCCTTGTGGTCCGGACCTCTCTCTTGCTGATGTATTTGGTCACCTTGAACGGAAAAGAAACCGCCTTGGAATTGCAGAATATAGTATCAGCCAGTCCACTCTGGAGACCATATTCAATCATTTTGCAGCCAACTCTTGA

Protein Analysis

1784

Amino Acids

198.09

Weight (kDa)

6.99

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC2_membrane_3 PF12698 72 - 337 2.2e-18 ABC-2 family transporter protein
ABC_tran PF00005 436 - 579 2.9e-31 ABC transporter
ABC2_membrane_3 PF12698 848 - 1250 9.6e-39 ABC-2 family transporter protein
ABC_tran PF00005 1343 - 1485 9.6e-27 ABC transporter
AAA_21 PF13304 1454 - 1520 9.3e-09 AAA domain, putative AbiEii toxin, Type IV TA system
R1_ABCA1 PF23321 1674 - 1735 5.2e-11 ABCA1-like, C-terminal R1 regulatory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1817, 2454, 3644, 4398
Acc36I ACCTGC 1 cut(s) 2959
AccB1I GGYRCC 1 cut(s) 4895
AccB7I CCANNNNNTGG 1 cut(s) 4241
AccI GTMKAC 2 cut(s) 2579, 3482
AccII CGCG 3 cut(s) 794, 1242, 4684
AccIII TCCGGA 1 cut(s) 5224
AclI AACGTT 1 cut(s) 163
AclWI GGATC 6 cut(s) 1841, 1854, 2399, 4520, 4562, 4575
AcoI YGGCCR 1 cut(s) 1722
AcsI RAATTY 4 cut(s) 64, 2888, 2972, 3664
AcuI CTGAAG 3 cut(s) 289, 1503, 2375
AcvI CACGTG 1 cut(s) 485
AfaI GTAC 6 cut(s) 285, 1128, 3062, 4035, 4128, 4389
AfiI CCNNNNNNNGG 9 cut(s) 1123, 1520, 1838, 3783, 4000, 4241, 4479, 4480, 4559
AflII CTTAAG 1 cut(s) 3674
AflIII ACRYGT 2 cut(s) 482, 2111
AhdI GACNNNNNGTC 1 cut(s) 3201
AhlI ACTAGT 1 cut(s) 1835
AjiI CACGTC 1 cut(s) 4295
AjnI CCWGG 7 cut(s) 1157, 2813, 3697, 3931, 4093, 5164, 5178
AjuI GAANNNNNNNTTGG 4 cut(s) 1622, 1654, 4875, 4907
AleI CACNNNNGTG 1 cut(s) 4010
AloI GAACNNNNNNTCC 2 cut(s) 2288, 2320
Alw21I GWGCWC 3 cut(s) 1003, 1638, 4682
Alw26I GTCTC 7 cut(s) 426, 626, 1070, 1785, 2385, 2631, 5312
Alw44I GTGCAC 1 cut(s) 4678
AlwI GGATC 6 cut(s) 1841, 1854, 2399, 4520, 4562, 4575
AlwNI CAGNNNCTG 1 cut(s) 3084
Ama87I CYCGRG 1 cut(s) 205
Aor13HI TCCGGA 1 cut(s) 5224
AoxI GGCC 8 cut(s) 244, 357, 1501, 1722, 2816, 3774, 4705, 4876
ApaLI GTGCAC 1 cut(s) 4678
ApoI RAATTY 4 cut(s) 64, 2888, 2972, 3664
AseI ATTAAT 1 cut(s) 1877
Asp700I GAANNNNTTC 3 cut(s) 2236, 3683, 3864
AspLEI GCGC 3 cut(s) 794, 1863, 4686
AsuHPI GGTGA 6 cut(s) 1414, 3130, 4235, 4786, 5057, 5246
AsuII TTCGAA 1 cut(s) 2175
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 9 cut(s) 155, 449, 858, 2792, 2948, 3451, 3991, 5222, 5227
AxyI CCTNAGG 1 cut(s) 2309
BaeGI GKGCMC 2 cut(s) 2375, 4682
BamHI GGATCC 2 cut(s) 1846, 4567
BanI GGYRCC 1 cut(s) 4895
BanII GRGCYC 1 cut(s) 3957
BauI CACGAG 2 cut(s) 3147, 4950
BbrPI CACGTG 1 cut(s) 485
BbsI GAAGAC 5 cut(s) 845, 1004, 1196, 4022, 4202
Bbv12I GWGCWC 3 cut(s) 1003, 1638, 4682
BbvCI CCTCAGC 3 cut(s) 1596, 1938, 4475
BceAI ACGGC 3 cut(s) 3233, 4647, 4803
BcgI CGANNNNNNTGC 6 cut(s) 1474, 1508, 4669, 4703, 5051, 5085
BciT130I CCWGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
BciVI GTATCC 3 cut(s) 1164, 1261, 2853
BclI TGATCA 4 cut(s) 148, 576, 2725, 3556
BcoDI GTCTC 7 cut(s) 426, 626, 1070, 1785, 2385, 2631, 5312
BcuI ACTAGT 1 cut(s) 1835
BfaI CTAG 8 cut(s) 408, 1118, 1134, 1454, 1836, 3887, 4029, 4769
BfmI CTRYAG 7 cut(s) 683, 954, 2514, 2673, 3243, 3297, 4533
BfrI CTTAAG 1 cut(s) 3674
BfuAI ACCTGC 1 cut(s) 2959
BfuI GTATCC 3 cut(s) 1164, 1261, 2853
BglI GCCNNNNNGGC 2 cut(s) 1967, 2072
BglII AGATCT 1 cut(s) 2385
BlpI GCTNAGC 1 cut(s) 3324
Bme1390I CCNGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
Bme18I GGWCC 9 cut(s) 155, 449, 858, 2792, 2948, 3451, 3991, 5222, 5227
BmeRI GACNNNNNGTC 1 cut(s) 3201
BmeT110I CYCGRG 1 cut(s) 205
BmgBI CACGTC 1 cut(s) 4295
BmiI GGNNCC 7 cut(s) 859, 860, 1848, 2949, 4045, 4569, 4897
BmrFI CCNGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
BmrI ACTGGG 2 cut(s) 278, 977
BmuI ACTGGG 2 cut(s) 278, 977
BpiI GAAGAC 5 cut(s) 845, 1004, 1196, 4022, 4202
BplI GAGNNNNNCTC 2 cut(s) 606, 638
BpmI CTGGAG 4 cut(s) 308, 1779, 3681, 5336
Bpu10I CCTNAGC 4 cut(s) 1287, 1596, 1938, 4475
Bpu1102I GCTNAGC 1 cut(s) 3324
Bpu14I TTCGAA 1 cut(s) 2175
BpuEI CTTGAG 7 cut(s) 2690, 2696, 3881, 4382, 4653, 4784, 5215
Bsa29I ATCGAT 2 cut(s) 227, 4747
BsaAI YACGTR 2 cut(s) 485, 2774
BsaI GGTCTC 3 cut(s) 626, 2631, 5312
BsaJI CCNNGG 5 cut(s) 434, 1157, 3931, 5179, 5278
BsaWI WCCGGW 2 cut(s) 4390, 5224
BsaXI ACNNNNNCTCC 4 cut(s) 123, 153, 2288, 2318
Bsc4I CCNNNNNNNGG 9 cut(s) 1123, 1520, 1838, 3783, 4000, 4241, 4479, 4480, 4559
Bse21I CCTNAGG 1 cut(s) 2309
Bse3DI GCAATG 5 cut(s) 933, 1269, 2555, 5087, 5178
BseAI TCCGGA 1 cut(s) 5224
BseBI CCWGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
BseCI ATCGAT 2 cut(s) 227, 4747
BseDI CCNNGG 5 cut(s) 434, 1157, 3931, 5179, 5278
BseLI CCNNNNNNNGG 9 cut(s) 1123, 1520, 1838, 3783, 4000, 4241, 4479, 4480, 4559
BseMI GCAATG 5 cut(s) 933, 1269, 2555, 5087, 5178
BseRI GAGGAG 2 cut(s) 1580, 2312
BseSI GKGCMC 2 cut(s) 2375, 4682
BseYI CCCAGC 3 cut(s) 360, 4190, 4731
BsgI GTGCAG 2 cut(s) 2040, 4275
Bsh1236I CGCG 3 cut(s) 794, 1242, 4684
BshFI GGCC 8 cut(s) 246, 359, 1503, 1724, 2818, 3776, 4707, 4878
BshNI GGYRCC 1 cut(s) 4895
BshVI ATCGAT 2 cut(s) 227, 4747
BsiHKAI GWGCWC 3 cut(s) 1003, 1638, 4682
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 2 cut(s) 4391, 5225
BslFI GGGAC 5 cut(s) 871, 2961, 3401, 4258, 4713
BslI CCNNNNNNNGG 9 cut(s) 1123, 1520, 1838, 3783, 4000, 4241, 4479, 4480, 4559
BsmAI GTCTC 7 cut(s) 426, 626, 1070, 1785, 2385, 2631, 5312
BsmBI CGTCTC 1 cut(s) 426
BsmFI GGGAC 5 cut(s) 871, 2961, 3401, 4258, 4713
BsmI GAATGC 3 cut(s) 177, 2593, 3127
BsnI GGCC 8 cut(s) 246, 359, 1503, 1724, 2818, 3776, 4707, 4878
Bso31I GGTCTC 3 cut(s) 626, 2631, 5312
BsoBI CYCGRG 1 cut(s) 205
Bsp119I TTCGAA 1 cut(s) 2175
Bsp1286I GDGCHC 5 cut(s) 1003, 1638, 2375, 3957, 4682
Bsp13I TCCGGA 1 cut(s) 5224
Bsp1720I GCTNAGC 1 cut(s) 3324
Bsp19I CCATGG 1 cut(s) 434
Bsp68I TCGCGA 1 cut(s) 1242
BspANI GGCC 8 cut(s) 246, 359, 1503, 1724, 2818, 3776, 4707, 4878
BspDI ATCGAT 2 cut(s) 227, 4747
BspEI TCCGGA 1 cut(s) 5224
BspFNI CGCG 3 cut(s) 794, 1242, 4684
BspHI TCATGA 2 cut(s) 145, 2401
BspLI GGNNCC 7 cut(s) 859, 860, 1848, 2949, 4045, 4569, 4897
BspMAI CTGCAG 3 cut(s) 687, 2677, 3247
BspMI ACCTGC 1 cut(s) 2959
BspPI GGATC 6 cut(s) 1841, 1854, 2399, 4520, 4562, 4575
BspQI GCTCTTC 3 cut(s) 1455, 2485, 3741
BspT104I TTCGAA 1 cut(s) 2175
BspT107I GGYRCC 1 cut(s) 4895
BspTI CTTAAG 1 cut(s) 3674
BspTNI GGTCTC 3 cut(s) 626, 2631, 5312
BsrDI GCAATG 5 cut(s) 933, 1269, 2555, 5087, 5178
BssECI CCNNGG 5 cut(s) 434, 1157, 3931, 5179, 5278
BssSI CACGAG 2 cut(s) 3147, 4950
BssT1I CCWWGG 2 cut(s) 434, 5278
Bst2BI CACGAG 2 cut(s) 3147, 4950
Bst2UI CCWGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
Bst6I CTCTTC 5 cut(s) 1455, 1763, 2485, 3741, 3905
BstAFI CTTAAG 1 cut(s) 3674
BstAPI GCANNNNNTGC 3 cut(s) 2063, 2627, 3287
BstBAI YACGTR 2 cut(s) 485, 2774
BstBI TTCGAA 1 cut(s) 2175
BstDSI CCRYGG 1 cut(s) 434
BstEII GGTNACC 1 cut(s) 5252
BstENI CCTNNNNNAGG 3 cut(s) 1121, 1518, 3998
BstFNI CGCG 3 cut(s) 794, 1242, 4684
BstHHI GCGC 3 cut(s) 794, 1863, 4686
BstMAI GTCTC 7 cut(s) 426, 626, 1070, 1785, 2385, 2631, 5312
BstNI CCWGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
BstNSI RCATGY 4 cut(s) 2115, 3095, 4112, 4454
BstPI GGTNACC 1 cut(s) 5252
BstSCI CCNGG 7 cut(s) 1157, 2813, 3697, 3931, 4093, 5164, 5178
BstSFI CTRYAG 7 cut(s) 683, 954, 2514, 2673, 3243, 3297, 4533
BstSLI GKGCMC 2 cut(s) 2375, 4682
BstUI CGCG 3 cut(s) 794, 1242, 4684
BstV2I GAAGAC 5 cut(s) 845, 1004, 1196, 4022, 4202
BstX2I RGATCY 4 cut(s) 1846, 2385, 4525, 4567
BstXI CCANNNNNNTGG 2 cut(s) 3461, 3988
BstYI RGATCY 4 cut(s) 1846, 2385, 4525, 4567
Bsu15I ATCGAT 2 cut(s) 227, 4747
Bsu36I CCTNAGG 1 cut(s) 2309
BsuI GTATCC 3 cut(s) 1164, 1261, 2853
BsuRI GGCC 8 cut(s) 246, 359, 1503, 1724, 2818, 3776, 4707, 4878
BsuTUI ATCGAT 2 cut(s) 227, 4747
BtgI CCRYGG 1 cut(s) 434
BtrI CACGTC 1 cut(s) 4295
BtsI GCAGTG 3 cut(s) 941, 2061, 4641
BtuMI TCGCGA 1 cut(s) 1242
BveI ACCTGC 1 cut(s) 2959
CaiI CAGNNNCTG 1 cut(s) 3084
CciI TCATGA 2 cut(s) 145, 2401
CfoI GCGC 3 cut(s) 794, 1863, 4686
ClaI ATCGAT 2 cut(s) 227, 4747
CseI GACGC 1 cut(s) 448
Csp6I GTAC 6 cut(s) 284, 1127, 3061, 4034, 4127, 4388
CspCI CAANNNNNGTGG 2 cut(s) 2860, 2895
CviQI GTAC 6 cut(s) 284, 1127, 3061, 4034, 4127, 4388
DriI GACNNNNNGTC 1 cut(s) 3201
EaeI YGGCCR 1 cut(s) 1722
Eam1104I CTCTTC 5 cut(s) 1455, 1763, 2485, 3741, 3905
Eam1105I GACNNNNNGTC 1 cut(s) 3201
EarI CTCTTC 5 cut(s) 1455, 1763, 2485, 3741, 3905
EciI GGCGGA 1 cut(s) 4931
Eco130I CCWWGG 2 cut(s) 434, 5278
Eco147I AGGCCT 1 cut(s) 3776
Eco24I GRGCYC 1 cut(s) 3957
Eco31I GGTCTC 3 cut(s) 626, 2631, 5312
Eco32I GATATC 3 cut(s) 1488, 1606, 4602
Eco47I GGWCC 9 cut(s) 155, 449, 858, 2792, 2948, 3451, 3991, 5222, 5227
Eco57I CTGAAG 3 cut(s) 289, 1503, 2375
Eco72I CACGTG 1 cut(s) 485
Eco81I CCTNAGG 1 cut(s) 2309
Eco88I CYCGRG 1 cut(s) 205
Eco91I GGTNACC 1 cut(s) 5252
EcoNI CCTNNNNNAGG 3 cut(s) 1121, 1518, 3998
EcoO109I RGGNCCY 3 cut(s) 155, 858, 2948
EcoO65I GGTNACC 1 cut(s) 5252
EcoRII CCWGG 7 cut(s) 1157, 2813, 3697, 3931, 4093, 5164, 5178
EcoRV GATATC 3 cut(s) 1488, 1606, 4602
EcoT14I CCWWGG 2 cut(s) 434, 5278
EcoT22I ATGCAT 2 cut(s) 1349, 4927
EcoT38I GRGCYC 1 cut(s) 3957
ErhI CCWWGG 2 cut(s) 434, 5278
Esp3I CGTCTC 1 cut(s) 426
FalI AAGNNNNNCTT 6 cut(s) 1622, 1654, 2448, 2480, 4344, 4376
FaqI GGGAC 5 cut(s) 871, 2961, 3401, 4258, 4713
FauI CCCGC 2 cut(s) 1026, 4472
FauNDI CATATG 3 cut(s) 676, 3383, 4843
FbaI TGATCA 4 cut(s) 148, 576, 2725, 3556
FblI GTMKAC 2 cut(s) 2579, 3482
FriOI GRGCYC 1 cut(s) 3957
FspBI CTAG 8 cut(s) 408, 1118, 1134, 1454, 1836, 3887, 4029, 4769
GlaI GCGC 3 cut(s) 793, 1862, 4685
GsaI CCCAGC 3 cut(s) 364, 4194, 4735
GsuI CTGGAG 4 cut(s) 308, 1779, 3681, 5336
HaeIII GGCC 8 cut(s) 246, 359, 1503, 1724, 2818, 3776, 4707, 4878
HapII CCGG 2 cut(s) 4391, 5225
HgaI GACGC 1 cut(s) 448
HhaI GCGC 3 cut(s) 794, 1863, 4686
Hin6I GCGC 3 cut(s) 792, 1861, 4684
HinP1I GCGC 3 cut(s) 792, 1861, 4684
HindIII AAGCTT 3 cut(s) 2147, 4811, 4864
HpaI GTTAAC 1 cut(s) 1204
HpaII CCGG 2 cut(s) 4391, 5225
HphI GGTGA 6 cut(s) 1414, 3130, 4235, 4786, 5057, 5246
HpyCH4IV ACGT 7 cut(s) 163, 484, 821, 2773, 3310, 3742, 4294
HpySE526I ACGT 7 cut(s) 163, 484, 821, 2773, 3310, 3742, 4294
HspAI GCGC 3 cut(s) 792, 1861, 4684
KflI GGGWCCC 1 cut(s) 858
Kpn2I TCCGGA 1 cut(s) 5224
Ksp22I TGATCA 4 cut(s) 148, 576, 2725, 3556
KspAI GTTAAC 1 cut(s) 1204
LguI GCTCTTC 3 cut(s) 1455, 2485, 3741
LmnI GCTCC 6 cut(s) 131, 1020, 1466, 1633, 4187, 4280
MaeI CTAG 8 cut(s) 408, 1118, 1134, 1454, 1836, 3887, 4029, 4769
MaeII ACGT 7 cut(s) 163, 484, 821, 2773, 3310, 3742, 4294
MaeIII GTNAC 6 cut(s) 746, 1089, 2284, 2737, 3790, 5252
MfeI CAATTG 4 cut(s) 2922, 4509, 4572, 5136
MflI RGATCY 4 cut(s) 1846, 2385, 4525, 4567
MhlI GDGCHC 5 cut(s) 1003, 1638, 2375, 3957, 4682
MlyI GAGTC 6 cut(s) 1186, 2387, 2741, 3600, 4830, 4898
MmeI TCCRAC 6 cut(s) 503, 998, 2107, 2837, 4259, 4681
Mph1103I ATGCAT 2 cut(s) 1349, 4927
MroI TCCGGA 1 cut(s) 5224
MroXI GAANNNNTTC 3 cut(s) 2236, 3683, 3864
MslI CAYNNNNRTG 4 cut(s) 2019, 3382, 3614, 4010
MspA1I CMGCKG 3 cut(s) 400, 845, 4479
MspCI CTTAAG 1 cut(s) 3674
MspI CCGG 2 cut(s) 4391, 5225
MspR9I CCNGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
MunI CAATTG 4 cut(s) 2922, 4509, 4572, 5136
Mva1269I GAATGC 3 cut(s) 177, 2593, 3127
MvaI CCWGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
MvnI CGCG 3 cut(s) 794, 1242, 4684
NcoI CCATGG 1 cut(s) 434
NdeI CATATG 3 cut(s) 676, 3383, 4843
NlaIV GGNNCC 7 cut(s) 859, 860, 1848, 2949, 4045, 4569, 4897
NmuCI GTSAC 3 cut(s) 746, 2284, 5252
NruI TCGCGA 1 cut(s) 1242
NsiI ATGCAT 2 cut(s) 1349, 4927
NspI RCATGY 4 cut(s) 2115, 3095, 4112, 4454
NspV TTCGAA 1 cut(s) 2175
OliI CACNNNNGTG 1 cut(s) 4010
PaeI GCATGC 3 cut(s) 3095, 4112, 4454
PagI TCATGA 2 cut(s) 145, 2401
PceI AGGCCT 1 cut(s) 3776
PciI ACATGT 1 cut(s) 2111
PciSI GCTCTTC 3 cut(s) 1455, 2485, 3741
PctI GAATGC 3 cut(s) 177, 2593, 3127
PdmI GAANNNNTTC 3 cut(s) 2236, 3683, 3864
PflMI CCANNNNNTGG 1 cut(s) 4241
PfoI TCCNGGA 2 cut(s) 3697, 4093
PleI GAGTC 6 cut(s) 1186, 2386, 2740, 3600, 4830, 4898
PmaCI CACGTG 1 cut(s) 485
PmlI CACGTG 1 cut(s) 485
PpsI GAGTC 6 cut(s) 1186, 2386, 2740, 3600, 4830, 4898
Ppu21I YACGTR 2 cut(s) 485, 2774
PpuMI RGGWCCY 3 cut(s) 155, 858, 2948
PscI ACATGT 1 cut(s) 2111
PshBI ATTAAT 1 cut(s) 1877
PsiI TTATAA 4 cut(s) 1817, 2454, 3644, 4398
Psp1406I AACGTT 1 cut(s) 163
Psp5II RGGWCCY 3 cut(s) 155, 858, 2948
Psp6I CCWGG 7 cut(s) 1157, 2813, 3697, 3931, 4093, 5164, 5178
PspCI CACGTG 1 cut(s) 485
PspEI GGTNACC 1 cut(s) 5252
PspFI CCCAGC 3 cut(s) 360, 4190, 4731
PspGI CCWGG 7 cut(s) 1157, 2813, 3697, 3931, 4093, 5164, 5178
PspN4I GGNNCC 7 cut(s) 859, 860, 1848, 2949, 4045, 4569, 4897
PspPPI RGGWCCY 3 cut(s) 155, 858, 2948
PstI CTGCAG 3 cut(s) 687, 2677, 3247
PstNI CAGNNNCTG 1 cut(s) 3084
PsuI RGATCY 4 cut(s) 1846, 2385, 4525, 4567
PvuII CAGCTG 1 cut(s) 845
RruI TCGCGA 1 cut(s) 1242
RsaI GTAC 6 cut(s) 285, 1128, 3062, 4035, 4128, 4389
RsaNI GTAC 6 cut(s) 284, 1127, 3061, 4034, 4127, 4388
RseI CAYNNNNRTG 4 cut(s) 2019, 3382, 3614, 4010
SalI GTCGAC 2 cut(s) 2578, 3481
SapI GCTCTTC 3 cut(s) 1455, 2485, 3741
SchI GAGTC 6 cut(s) 1186, 2387, 2741, 3600, 4830, 4898
ScrFI CCNGG 7 cut(s) 1159, 2815, 3699, 3933, 4095, 5166, 5180
SduI GDGCHC 5 cut(s) 1003, 1638, 2375, 3957, 4682
SfcI CTRYAG 7 cut(s) 683, 954, 2514, 2673, 3243, 3297, 4533
SfuI TTCGAA 1 cut(s) 2175
SinI GGWCC 9 cut(s) 155, 449, 858, 2792, 2948, 3451, 3991, 5222, 5227
SmiMI CAYNNNNRTG 4 cut(s) 2019, 3382, 3614, 4010
SmlI CTYRAG 8 cut(s) 2705, 2711, 3674, 3896, 4361, 4668, 4763, 5194
SmoI CTYRAG 8 cut(s) 2705, 2711, 3674, 3896, 4361, 4668, 4763, 5194
SpeI ACTAGT 1 cut(s) 1835
SphI GCATGC 3 cut(s) 3095, 4112, 4454
SseBI AGGCCT 1 cut(s) 3776
SspI AATATT 4 cut(s) 1549, 1647, 4340, 5097
SspMI CTAG 8 cut(s) 408, 1118, 1134, 1454, 1836, 3887, 4029, 4769
StuI AGGCCT 1 cut(s) 3776
StyD4I CCNGG 7 cut(s) 1157, 2813, 3697, 3931, 4093, 5164, 5178
StyI CCWWGG 2 cut(s) 434, 5278
TaiI ACGT 7 cut(s) 166, 487, 824, 2776, 3313, 3745, 4297
TaqI TCGA 9 cut(s) 113, 227, 1083, 2175, 2579, 3482, 3949, 4747, 5071
TaqII GACCGA 1 cut(s) 2681
TauI GCSGC 1 cut(s) 5008
TseFI GTSAC 3 cut(s) 746, 2284, 5252
Tsp45I GTSAC 3 cut(s) 746, 2284, 5252
TspGWI ACGGA 5 cut(s) 441, 1588, 3522, 4253, 5278
Van91I CCANNNNNTGG 1 cut(s) 4241
Vha464I CTTAAG 1 cut(s) 3674
VneI GTGCAC 1 cut(s) 4678
VpaK11BI GGWCC 9 cut(s) 155, 449, 858, 2792, 2948, 3451, 3991, 5222, 5227
VspI ATTAAT 1 cut(s) 1877
XagI CCTNNNNNAGG 3 cut(s) 1121, 1518, 3998
XapI RAATTY 4 cut(s) 64, 2888, 2972, 3664
XceI RCATGY 4 cut(s) 2115, 3095, 4112, 4454
XcmI CCANNNNNNNNNTGG 4 cut(s) 2068, 3011, 3599, 5186
XmiI GTMKAC 2 cut(s) 2579, 3482
XmnI GAANNNNTTC 3 cut(s) 2236, 3683, 3864
XspI CTAG 8 cut(s) 408, 1118, 1134, 1454, 1836, 3887, 4029, 4769
Zsp2I ATGCAT 2 cut(s) 1349, 4927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.