Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0005424

Physical Location & Seq

Genomic Coordinates Forward (+)

28481669 .. 28503908

Gene Structure 22240 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG283300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5664 bp

ATGGGGACCGGAACGAGACAGCTCAAGGCTATGCTTCGCAAGAATTGGCTTCTCAAAATTCGTCACCCTTTCGTCACCTGCGCTGAGATATTACTCCCCACTGTAGTAATGTTGATGCTCATAGCTGTAAGGACACGTGTTGATACGCAGATTCACCCTTCACAGCCATATATCCGGAAAGGGATGTTGGTGGAAGTGGGCAAAGGAATATCCCCTAATTTTGAGCAAGTGTTGGAATTACTATTGAAGAAGGAAGAGATTTTGGCTTTTGCACCAGACACAGAAGAGACAAGGTCAATGATCAACGTGATGTCGATGAAATTTCCTCTCTTGAAGCGTGTTTCTAGAGTTTACAAAGATGAGCAGGAGCTGGAAACCTATATACGCTCAAATCTCTATGGTACCTGCAATCAAATCATGAATTGCTCGAACCCAAAAATCAAAGGAGCAGTAGTATTTCACGATCAAGGTCCTCAACTTTTCGATTATAGCATACGACTTAATCACACATGGGCTTTCTCGGGATTTCCTGATGTTAAATCGATCATGGACACAAATGGCCCTTACTTAAATGACTTGGAACTGGGCGTGAATACTGTACCGACAATGCAATACAGCTGCAGTGGATTTTTAACTCTCCAGCAAGCACTAGACTCTTTTATAATTTTTGCAGCCCAGCAATCGGATACCAGAAACATAGAACTTCCTACTTCGCTGTCCTTTAGCACACCTTCCTCTCTCAAAGTTCCTTGGACGCGATATGGTCCGTCAAACATCCGTATAGTCCCGTTTCCGACTCGTGAATACACTGATGATGAGTTCCAGTCCATCATAAAAAGTGTCATGGGTGTACTGTATCTTTTGGGATTTCTCTATCCAATTTCGCGCTTGATCAGCTATTCTGTGTTTGAGAAGGAACAAAAGATAAGAGAAGGTCTTTCTATGATGGGGCTGAAAGATGGGGTATTCCATCTATCGTGGTTCATTGCATATGCTTTGCAGTTCGCAGTTTCTTCTCTTATTATTACAGTTTGCACGATGGATAGCCTTTTCAAGTATAGTGACAAGTCAGTGGTGTTCATTTACTTCTTCTTTTTCGGAATAAGCGCGATTATGCTGTCATTTTTAATCTCTACATTCTTCGAACGAGCAAAGACAGCAGTTGCGGTTGGGACCCTTGCTTTCTTGGGTGCTTTTTTCCCATACTACTCTGTCAATGACGAGGCTGTTCCTATGATTCTAAAGGTCATTGCCTCTCTGCTTTCACCAACGGCTTTTGCTCTGGGGTCAATCAACTTTGCTGATTATGAGCGTGCTCATGTTGGCCTTCGTTGGAGCAATATATGGCGGGCATCATCCGGAGTGAATTTTTCAGTCTGTCTCCTGATGATGTTGCTCGATGCGTTGCTGTACTGTGTAATTGGTCTTTACCTAGATAAGGTACTTCCGAGAGAGAATGGAGTTAGGTATCCCTGGAACTTCATTTTTCAGAAGTGCTTTTGGAAGAATCCAAATATTAACAAACACCACAACTCCAGTCCAGAAGTTCATATTCGTGACAAGGTATCCCAGAAGGCAATGTTTTCTCGGAAAGAAAATGCTAAAGCTGCTGTGGAAGCAATAACCTTCGATATGAGACAACAAGAGCTTGACCATAGATGCATCCAAATAAGGAATCTGCATAAGGTTTATGCTAGTAAAAAGGGGAAATGTTGTGCTGTCAACTCATTACAGCTCACCATGTATGAAAATCAGATTCTTGCTCTTCTAGGACATAATGGAGCTGGTAAAAGCACAACGATATCAATGCTTGTTGGCCTTCTTCGTCCTACTTCAGGGGATGCTATGGTGTTTGGAAAAAATATTATGACAGACATGGAGGAGATACGGAAAGAGTTGGGTGTATGCCCTCAGCATGATATCCTTTTTCCAGAATTGACCGTGAAGGAGCACTTGGAAATATTTGCTATATTGAAAGGTGTGCAGGAAGATTTTCTGAACAGCGTTGTTATTGATATGGTTGACCAAGTAGGGTTGGCCGATAAAATCAACACTGCTGTTATGGCTCTTTCTGGAGGAATGAAGAGGAAGTTGTCACTTGGAATTGCACTAATTGGAAATAGTAAGGTTATAATTCTTGATGAACCTACTAGTGGAATGGATCCATACTCTATGCGCTTGACTTGGCAATTAATAAAAAAGATTAGAAAGGGCAGGATAGTATTACTGACAACACACTCAATGGATGAAGCTGAGGCACTTGGAGATCGCATAGCCATCATGGCCAATGGTTCTTTGAAATGCTGCGGAAGTTCCCTTTTCTTGAAGCATCAGTATGGGGTTGGTTATACTCTAACTTTAGTGAAGTCTGCACCCACTGCCTCTATGGCTGCTGATATTGTTTTCCGCCATATCCCATCGGCAACGTGTGTGAGTGAGGTTGGAACTGAGATTTCCTTTAAGCTTCCATTGGCATCCTCAACATCCTTCGAAAGTATGTTTAGAGAAATTGAGAGTTGCATGGGATCATCCACATCAAACTTAGAAACAAGTTGTGATGAGAAGGACTATATTGGCATTGAAAGTTATGGCATATCTGTCACAACTTTGGAGGAGGTGTTCCTGAGGGTTGCAGGATGCGATTATGACGAAGCTGCTTGTTTTGACCAGAAGAATGGTCCACTGTGCCCTGAGTCTCTGATTTCTCAAACTTCTCATGATCCTACCCACAAACAGATTCTTCACTCGAAGAAATCTTTTGCTTATTATAAAGAGATTCTTGGGGTCTTATTTACAATGGTGGGAAGAGCCTGTGGTTTAATTTTTGCTACAGTGTTAAGTTTCTTAAACTTCTTAGGTGTGCAATGCTGTGGCTGTTGTATCATTTCAAGGTCAACATTTTGGAGGCATTCTAAAGTATTATTCATAAAGAGGGCAATATCTGCCCGCAGAGACCGTAAAACAATTGTTTTCCAGCTTGTAATTCCTGCAGTTTTCTTATTTTTTGGTCTTTTATTTCTCAAGCTCAAGCCACATCCTGATCAGGAGTCTGTTACCTTCACAACTTCATATTTTAATCCACTATTACGTGGTGGTGGTGGTGGTGGTCCAATTCCTTTTGATCTATCCCGGCCTATTGCACAGGAGGTTGCAGAATACATAGAAGGGGGTTGGATACAAACCTTCAAACCTAGTGGGTATAACTTCCCCAACTCAGAGAAGGCATTGAATGATGCAATTGAGGTAGCAGGGGAAACTCTGGGACCTGCTTTACTTTCAATGAGTGAATTTTTGATGTCCAGCTTTAATGAATCTTACCAGTCAAGGTATGGCGCAGTTGTGATGGATGATCAGAGTGATGATGGGAGTTTAGGGTACACTGTGCTACACAACAGTTCCTGTCAGCATGCTGCCCCAACCTTTATCAATTTAGTAAATGCTGCAATTCTTCGTCTTGCTTCTCGTAATAAGAATGTGACAATTCAAACACGCAATCACCCTTTGCCAATGACAGAAAGTCAACATCTGCAACGGCATGATTTGGATGCTTTCTCTGCTGCAGTTATTGTTAGTATCGCCTTCTCCTTCATTCCTGCATCATTTGCTGTTCCTATAGTGAAGGAACGTGAAGTAAAAGCTAAACACCAACAACTTATTAGTGGAGTTTCCATACTGTCATATTGGACTTCTACATACATTTGGGACTTCATCAGCTTCCTCTTTCCTTCATCTTTTGCCATAGTTCTTTTTTACATATTTGGTCTGGACCAGTTTATTGGAAGGGGATGTTTACTGTCGACTGTCGTCATGTTTTTAGCATATGGATTAGCGATTGCATCATCAACATACTGCCTTACGTTTTTCTTTTCTGATCACACTATGGCTCAGAATGTGGTTCTCTTGGTCCACTTTTTCACGGGACTCATTCTTATGGTTATCTCATTCATTATGGGGCTTATAAAGACGACGACAAGTGCAAATTCTTTTCTTAAGAACTTTTTCAGATTATCTCCAGGATTTTGCTTTGCTGACGGACTTGCTTCACTGGCTCTTCTACGTCAAGATATGAAAAATAAGAGCAATAAGGCCTTCGATTGGAATGTTACTGGTGGCTCAATCTGCTACCTTTGTATTGAGAGCATTTGTTACTTCCTTTTGGCACTCGGTCTTGAAATCTTGCCTTTCAATAAATTGACACTAGCCACACTCAAGGAGTGGTGGAAGAGTATTAGAAGTATCCCACCTAGCACTTCAAGTTATCGAGAACCCCTTCTGACTTCTTCCGCAGAATCTGTCACTCTGGACCTTGATGAGGACACAGATGTGAAGGCAGAAAGAACTAGGGTACTTTCAGGTTCCATTGACAATGCCATCATCTACTTGCGTAATCTTCGTAAGGTGTATCCAGGAGGACAGCAGCATGCTACAAAGGTTGCGGTACATTCATTGACTTTTTCAGTTCAAGAAGGGGAATGTTTCGGTTTTTTAGGAACAAATGGAGCTGGGAAGACCACTACTCTGTCTATGTTGACTGGTGAGGAATCTCCTACGGATGGGACTGCCTGTATATTTGGCAAGGATATTTCTTCCAATCCCAAGGCTGCTCGTCAACATATTGGGTTTTGCCCACAGTTTGATGCTCTGTTAGAATATTTAACTGTTCAAGAGCATCTTGAGCTTTATGCCACGATAAAAGGAGTACCAGATAATAAGATGGCTGAGGTTGTAATGGAAAAGTTGGTGGAGTTTGACTTGTTAAAGCATGCCGGCAAGCCATCATTTTCCCTCAGTGGGGGAAACAAGCGAAAATTATCTGTCGCAATTGCTATGATTGGAGATCCTCCTATAGTTATTCTTGATGAACCATCAACAGGTATGGATCCAATTGCCAAAAGATTCATGTGGGAAGTGATATCTCGTTTATCGACCAGACGAGGAAAGACAGCAGTGATACTGACTACCCATAGTATGAATGAAGCTCAAGCTCTGTGCACCCGTATCGGAATCATGGTTGGAGGCCAATTACGGTGCATTGGAAGTCCCCAGCATTTAAAAACTCGATTTGGAAATCATCTTGAGCTAGAGGTTAAACCTATTGAAGTCAGCTCTGTGGACTTGGACAAGCTTTGCCGAGTTATCCAAGAAAGGCTCTCGTATGTTCCTTCTCATCCAATGAGCTTACTTGATGGCCTTGAAGTCTGCATTGGTGCCACTGACTCCATTGTGGCAGAAAATGCATCGGTGGCAGAGATTAGTTTATCGCGAGAAATGATAATTATGATTGGACAATGGCTTGGGAATGAAGAAAGAATAAAGCCGCTTATATCTCCAACACCACTTTCAGATGGGGTGCTTGGTGAACAGTTATTTGAGCAGTTAGATCGAGATGGTGGAATACCATTGCCAGTATTTTCTGAATGGTGGCTGTTCAATGACAAGTTTTCAGCAATCGATTCATTTGTCCTATCATCATTTACTGGTGCAATGTTCGAGGGAATGAATGGCTTGAGTGTTAAATATCAGTTGCCTTGTGGTCCGCACCTCTCTCTTGCTGATGTATTTGGACACCTTGAACGGAAAAGAAACCGCCTTGGCATAGCAGAGTACAGTATTAGCCAGTCCACTCTGGAGACCATATTCAATCATTTTGCAGCCAACTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006869 GO:0008150 GO:0010876 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0033036 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko02010 map02010

Pfam Domains

Protein Families

ABC2_membrane_3 ABC_tran

Protein Analysis

1887

Amino Acids

209.99

Weight (kDa)

7.1

Isoelectric Point (pI)

36.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC2_membrane_3	PF12698	211 - 476	2e-19	ABC-2 family transporter protein
ABC_tran	PF00005	575 - 718	1.3e-30	ABC transporter
ABC2_membrane_3	PF12698	987 - 1388	8.9e-40	ABC-2 family transporter protein
ABC_tran	PF00005	1481 - 1623	4.8e-28	ABC transporter
AAA_21	PF13304	1592 - 1658	7.6e-09	AAA domain, putative AbiEii toxin, Type IV TA system
R1_ABCA1	PF23321	1813 - 1873	4e-09	ABCA1-like, C-terminal R1 regulatory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005424)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41700 AT2G41700
fragaria_vesca	FvH4_6g24100 FvH4_6g24100
malus_domestica	MD12G1079400.v1.1 MD14G1075700.v1.1
prunus_persica	Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1
pyrus_communis	pycom12g06660
rosa_chinensis	RchiOBHm_Chr3g0482761
rosa_laevigata	RLG00000023331
rosa_multiflora	Rmu_co8213162.1_g000001 Rmu_sc0001542.1_g000002 Rmu_sc0021223.1_g000001
rosa_roxburghii	Rroxscaffold_6G00398990
rosa_rugosa	Rorug03G0198800 Rorug03G0198900
rosa_samantha	Rh3AG249700 Rh3BG285700 Rh3CG283300 Rh3DG279300
rosa_wichuraiana	Rw3G022560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	662, 2132, 2769, 3959
AarI	CACCTGC	1 cut(s)	86
Acc36I	ACCTGC	3 cut(s)	86, 413, 3274
Acc65I	GGTACC	1 cut(s)	401
AccB1I	GGYRCC	2 cut(s)	401, 5207
AccI	GTMKAC	1 cut(s)	3797
AccII	CGCG	4 cut(s)	757, 886, 1109, 5263
AccIII	TCCGGA	2 cut(s)	174, 1358
AclWI	GGATC	7 cut(s)	2156, 2169, 2533, 2714, 4832, 4874, 4887
AcoI	YGGCCR	2 cut(s)	2037, 2283
AcsI	RAATTY	5 cut(s)	57, 320, 1366, 3287, 3979
AcuI	CTGAAG	1 cut(s)	1818
AcvI	CACGTG	1 cut(s)	137
AflII	CTTAAG	1 cut(s)	3989
AflIII	ACRYGT	3 cut(s)	134, 136, 2426
AhdI	GACNNNNNGTC	1 cut(s)	3516
AhlI	ACTAGT	1 cut(s)	2150
AjnI	CCWGG	3 cut(s)	1472, 4012, 4405
AjuI	GAANNNNNNNTTGG	8 cut(s)	170, 202, 1937, 1969, 5187, 5219, 5323, 5355
AleI	CACNNNNGTG	1 cut(s)	4322
AloI	GAACNNNNNNTCC	2 cut(s)	2603, 2635
Alw21I	GWGCWC	3 cut(s)	1318, 1953, 4994
Alw26I	GTCTC	7 cut(s)	10, 281, 1385, 1630, 2700, 2946, 5624
Alw44I	GTGCAC	1 cut(s)	4990
AlwI	GGATC	7 cut(s)	2156, 2169, 2533, 2714, 4832, 4874, 4887
AlwNI	CAGNNNCTG	3 cut(s)	370, 3399, 4292
Ama87I	CYCGRG	1 cut(s)	520
Aor13HI	TCCGGA	2 cut(s)	174, 1358
AoxI	GGCC	9 cut(s)	559, 1324, 1816, 2037, 2283, 3131, 4086, 5017, 5188
ApaLI	GTGCAC	1 cut(s)	4990
ApoI	RAATTY	5 cut(s)	57, 320, 1366, 3287, 3979
AseI	ATTAAT	1 cut(s)	2192
Asp700I	GAANNNNTTC	3 cut(s)	1992, 3998, 4269
Asp718I	GGTACC	1 cut(s)	401
AspLEI	GCGC	5 cut(s)	83, 888, 1109, 2178, 3335
AsuC2I	CCSGG	1 cut(s)	3130
AsuHPI	GGTGA	8 cut(s)	56, 67, 146, 1257, 1729, 3488, 4547, 5369
AsuII	TTCGAA	2 cut(s)	1143, 2490
AvaI	CYCGRG	1 cut(s)	520
AxyI	CCTNAGG	1 cut(s)	2624
BaeGI	GKGCMC	2 cut(s)	2690, 4994
BalI	TGGCCA	1 cut(s)	2285
BamHI	GGATCC	2 cut(s)	2161, 4879
BanI	GGYRCC	2 cut(s)	401, 5207
BauI	CACGAG	1 cut(s)	798
BbrPI	CACGTG	1 cut(s)	137
BbsI	GAAGAC	1 cut(s)	4514
Bbv12I	GWGCWC	3 cut(s)	1318, 1953, 4994
BbvCI	CCTCAGC	3 cut(s)	1911, 2253, 4719
BceAI	ACGGC	2 cut(s)	1287, 3548
BcgI	CGANNNNNNTGC	6 cut(s)	1789, 1823, 4981, 5015, 5363, 5397
BciT130I	CCWGG	3 cut(s)	1474, 4014, 4407
BciVI	GTATCC	6 cut(s)	679, 1479, 1576, 3168, 4247, 4413
BclI	TGATCA	5 cut(s)	300, 891, 3040, 3349, 3871
BcnI	CCSGG	1 cut(s)	3130
BcoDI	GTCTC	7 cut(s)	10, 281, 1385, 1630, 2700, 2946, 5624
BcuI	ACTAGT	1 cut(s)	2150
BfmI	CTRYAG	7 cut(s)	102, 619, 2829, 2988, 3558, 3612, 4845
BfrI	CTTAAG	1 cut(s)	3989
BfuAI	ACCTGC	3 cut(s)	86, 413, 3274
BfuI	GTATCC	6 cut(s)	679, 1479, 1576, 3168, 4247, 4413
BglI	GCCNNNNNGGC	2 cut(s)	2282, 2387
Bme1390I	CCNGG	4 cut(s)	1474, 3130, 4014, 4407
BmeRI	GACNNNNNGTC	1 cut(s)	3516
BmeT110I	CYCGRG	1 cut(s)	520
BmiI	GGNNCC	9 cut(s)	7, 403, 1174, 1175, 2163, 3264, 4357, 4881, 5209
BmrFI	CCNGG	4 cut(s)	1474, 3130, 4014, 4407
BmrI	ACTGGG	1 cut(s)	593
BmuI	ACTGGG	1 cut(s)	593
BoxI	GACNNNNGTC	1 cut(s)	3803
BpiI	GAAGAC	1 cut(s)	4514
BpmI	CTGGAG	5 cut(s)	623, 1519, 2094, 3996, 5648
Bpu10I	CCTNAGC	3 cut(s)	1911, 2253, 4719
Bpu14I	TTCGAA	2 cut(s)	1143, 2490
BpuEI	CTTGAG	8 cut(s)	8, 3005, 3011, 4193, 4694, 4965, 5096, 5527
BpuMI	CCSGG	1 cut(s)	3130
Bsa29I	ATCGAT	2 cut(s)	542, 5451
BsaAI	YACGTR	2 cut(s)	137, 3089
BsaI	GGTCTC	2 cut(s)	2946, 5624
BsaJI	CCNNGG	4 cut(s)	749, 1472, 4596, 5590
BsaWI	WCCGGW	3 cut(s)	8, 174, 1358
BsaXI	ACNNNNNCTCC	6 cut(s)	438, 468, 1517, 1547, 2603, 2633
Bse118I	RCCGGY	1 cut(s)	4766
Bse21I	CCTNAGG	1 cut(s)	2624
Bse3DI	GCAATG	6 cut(s)	984, 1248, 1584, 2870, 5399, 5490
BseAI	TCCGGA	2 cut(s)	174, 1358
BseBI	CCWGG	3 cut(s)	1474, 4014, 4407
BseCI	ATCGAT	2 cut(s)	542, 5451
BseDI	CCNNGG	4 cut(s)	749, 1472, 4596, 5590
BseMI	GCAATG	6 cut(s)	984, 1248, 1584, 2870, 5399, 5490
BseRI	GAGGAG	2 cut(s)	1895, 2627
BseSI	GKGCMC	2 cut(s)	2690, 4994
BseYI	CCCAGC	3 cut(s)	675, 4502, 5043
BsgI	GTGCAG	2 cut(s)	2003, 2355
Bsh1236I	CGCG	4 cut(s)	757, 886, 1109, 5263
BshFI	GGCC	9 cut(s)	561, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
BshNI	GGYRCC	2 cut(s)	401, 5207
BshVI	ATCGAT	2 cut(s)	542, 5451
BsiHKAI	GWGCWC	3 cut(s)	1318, 1953, 4994
BsiHKCI	CYCGRG	1 cut(s)	520
BsiSI	CCGG	5 cut(s)	9, 175, 1359, 3130, 4767
BslFI	GGGAC	8 cut(s)	19, 770, 1186, 3276, 3716, 3933, 4570, 5025
BsmAI	GTCTC	7 cut(s)	10, 281, 1385, 1630, 2700, 2946, 5624
BsmFI	GGGAC	8 cut(s)	19, 770, 1186, 3276, 3716, 3933, 4570, 5025
BsmI	GAATGC	1 cut(s)	2908
BsnI	GGCC	9 cut(s)	561, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
Bso31I	GGTCTC	2 cut(s)	2946, 5624
BsoBI	CYCGRG	1 cut(s)	520
Bsp119I	TTCGAA	2 cut(s)	1143, 2490
Bsp1286I	GDGCHC	4 cut(s)	1318, 1953, 2690, 4994
Bsp13I	TCCGGA	2 cut(s)	174, 1358
Bsp68I	TCGCGA	1 cut(s)	5263
BspANI	GGCC	9 cut(s)	561, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
BspDI	ATCGAT	2 cut(s)	542, 5451
BspEI	TCCGGA	2 cut(s)	174, 1358
BspFNI	CGCG	4 cut(s)	757, 886, 1109, 5263
BspHI	TCATGA	2 cut(s)	417, 2716
BspLI	GGNNCC	9 cut(s)	7, 403, 1174, 1175, 2163, 3264, 4357, 4881, 5209
BspMAI	CTGCAG	3 cut(s)	623, 2992, 3562
BspMI	ACCTGC	3 cut(s)	86, 413, 3274
BspPI	GGATC	7 cut(s)	2156, 2169, 2533, 2714, 4832, 4874, 4887
BspQI	GCTCTTC	3 cut(s)	1770, 2800, 4056
BspT104I	TTCGAA	2 cut(s)	1143, 2490
BspT107I	GGYRCC	2 cut(s)	401, 5207
BspTI	CTTAAG	1 cut(s)	3989
BspTNI	GGTCTC	2 cut(s)	2946, 5624
BsrDI	GCAATG	6 cut(s)	984, 1248, 1584, 2870, 5399, 5490
BsrFI	RCCGGY	1 cut(s)	4766
BssAI	RCCGGY	1 cut(s)	4766
BssECI	CCNNGG	4 cut(s)	749, 1472, 4596, 5590
BssSI	CACGAG	1 cut(s)	798
BssT1I	CCWWGG	3 cut(s)	749, 4596, 5590
Bst2BI	CACGAG	1 cut(s)	798
Bst2UI	CCWGG	3 cut(s)	1474, 4014, 4407
Bst6I	CTCTTC	7 cut(s)	249, 279, 1770, 2078, 2800, 4056, 4217
BstAFI	CTTAAG	1 cut(s)	3989
BstAPI	GCANNNNNTGC	4 cut(s)	2378, 2942, 3602, 5234
BstBAI	YACGTR	2 cut(s)	137, 3089
BstBI	TTCGAA	2 cut(s)	1143, 2490
BstC8I	GCNNGC	9 cut(s)	645, 1314, 1350, 2947, 3408, 4422, 4764, 4768, 4772
BstENI	CCTNNNNNAGG	3 cut(s)	1436, 1833, 4310
BstFNI	CGCG	4 cut(s)	757, 886, 1109, 5263
BstHHI	GCGC	5 cut(s)	83, 888, 1109, 2178, 3335
BstMAI	GTCTC	7 cut(s)	10, 281, 1385, 1630, 2700, 2946, 5624
BstNI	CCWGG	3 cut(s)	1474, 4014, 4407
BstNSI	RCATGY	3 cut(s)	3410, 4424, 4766
BstPAI	GACNNNNGTC	1 cut(s)	3803
BstSCI	CCNGG	4 cut(s)	1472, 3128, 4012, 4405
BstSFI	CTRYAG	7 cut(s)	102, 619, 2829, 2988, 3558, 3612, 4845
BstSLI	GKGCMC	2 cut(s)	2690, 4994
BstUI	CGCG	4 cut(s)	757, 886, 1109, 5263
BstV2I	GAAGAC	1 cut(s)	4514
BstX2I	RGATCY	3 cut(s)	2161, 4837, 4879
BstXI	CCANNNNNNTGG	2 cut(s)	2675, 3776
BstYI	RGATCY	3 cut(s)	2161, 4837, 4879
Bsu15I	ATCGAT	2 cut(s)	542, 5451
Bsu36I	CCTNAGG	1 cut(s)	2624
BsuI	GTATCC	6 cut(s)	679, 1479, 1576, 3168, 4247, 4413
BsuRI	GGCC	9 cut(s)	561, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
BsuTUI	ATCGAT	2 cut(s)	542, 5451
BtsI	GCAGTG	4 cut(s)	628, 2052, 2376, 4953
BtuMI	TCGCGA	1 cut(s)	5263
BveI	ACCTGC	3 cut(s)	86, 413, 3274
Cac8I	GCNNGC	9 cut(s)	645, 1314, 1350, 2947, 3408, 4422, 4764, 4768, 4772
CaiI	CAGNNNCTG	3 cut(s)	370, 3399, 4292
CciI	TCATGA	2 cut(s)	417, 2716
CfoI	GCGC	5 cut(s)	83, 888, 1109, 2178, 3335
Cfr10I	RCCGGY	1 cut(s)	4766
ClaI	ATCGAT	2 cut(s)	542, 5451
CseI	GACGC	1 cut(s)	763
CspCI	CAANNNNNGTGG	2 cut(s)	3175, 3210
DraI	TTTAAA	1 cut(s)	5052
DriI	GACNNNNNGTC	1 cut(s)	3516
EaeI	YGGCCR	2 cut(s)	2037, 2283
Eam1104I	CTCTTC	7 cut(s)	249, 279, 1770, 2078, 2800, 4056, 4217
Eam1105I	GACNNNNNGTC	1 cut(s)	3516
EarI	CTCTTC	7 cut(s)	249, 279, 1770, 2078, 2800, 4056, 4217
EciI	GGCGGA	1 cut(s)	2396
Eco130I	CCWWGG	3 cut(s)	749, 4596, 5590
Eco147I	AGGCCT	1 cut(s)	4088
Eco31I	GGTCTC	2 cut(s)	2946, 5624
Eco32I	GATATC	3 cut(s)	1803, 1921, 4914
Eco57I	CTGAAG	1 cut(s)	1818
Eco72I	CACGTG	1 cut(s)	137
Eco81I	CCTNAGG	1 cut(s)	2624
Eco88I	CYCGRG	1 cut(s)	520
EcoNI	CCTNNNNNAGG	3 cut(s)	1436, 1833, 4310
EcoO109I	RGGNCCY	3 cut(s)	470, 1173, 3263
EcoRII	CCWGG	3 cut(s)	1472, 4012, 4405
EcoRV	GATATC	3 cut(s)	1803, 1921, 4914
EcoT14I	CCWWGG	3 cut(s)	749, 4596, 5590
EcoT22I	ATGCAT	2 cut(s)	1664, 5239
ErhI	CCWWGG	3 cut(s)	749, 4596, 5590
FalI	AAGNNNNNCTT	6 cut(s)	1937, 1969, 2763, 2795, 4656, 4688
FaqI	GGGAC	8 cut(s)	19, 770, 1186, 3276, 3716, 3933, 4570, 5025
FauI	CCCGC	2 cut(s)	1341, 2954
FauNDI	CATATG	2 cut(s)	991, 3820
FbaI	TGATCA	5 cut(s)	300, 891, 3040, 3349, 3871
FblI	GTMKAC	1 cut(s)	3797
GlaI	GCGC	5 cut(s)	82, 887, 1108, 2177, 3334
GsaI	CCCAGC	3 cut(s)	679, 4506, 5047
GsuI	CTGGAG	5 cut(s)	623, 1519, 2094, 3996, 5648
HaeIII	GGCC	9 cut(s)	561, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
HapII	CCGG	5 cut(s)	9, 175, 1359, 3130, 4767
HgaI	GACGC	1 cut(s)	763
HhaI	GCGC	5 cut(s)	83, 888, 1109, 2178, 3335
Hin6I	GCGC	5 cut(s)	81, 886, 1107, 2176, 3333
HinP1I	GCGC	5 cut(s)	81, 886, 1107, 2176, 3333
HincII	GTYRAC	7 cut(s)	1723, 2023, 2895, 3521, 3798, 4530, 4610
HindII	GTYRAC	7 cut(s)	1723, 2023, 2895, 3521, 3798, 4530, 4610
HindIII	AAGCTT	2 cut(s)	2462, 5123
HpaII	CCGG	5 cut(s)	9, 175, 1359, 3130, 4767
HphI	GGTGA	8 cut(s)	56, 67, 146, 1257, 1729, 3488, 4547, 5369
Hpy99I	CGWCG	1 cut(s)	3970
HpyCH4IV	ACGT	7 cut(s)	136, 306, 2426, 3088, 3625, 3857, 4057
HpySE526I	ACGT	7 cut(s)	136, 306, 2426, 3088, 3625, 3857, 4057
HspAI	GCGC	5 cut(s)	81, 886, 1107, 2176, 3333
KflI	GGGWCCC	1 cut(s)	1173
Kpn2I	TCCGGA	2 cut(s)	174, 1358
KpnI	GGTACC	1 cut(s)	405
KroI	GCCGGC	1 cut(s)	4766
KroNI	GCCGGC	1 cut(s)	4768
Ksp22I	TGATCA	5 cut(s)	300, 891, 3040, 3349, 3871
LguI	GCTCTTC	3 cut(s)	1770, 2800, 4056
LmnI	GCTCC	6 cut(s)	367, 446, 1335, 1781, 1948, 4499
MaeII	ACGT	7 cut(s)	136, 306, 2426, 3088, 3625, 3857, 4057
MfeI	CAATTG	4 cut(s)	2964, 3237, 4821, 4884
MflI	RGATCY	3 cut(s)	2161, 4837, 4879
MhlI	GDGCHC	4 cut(s)	1318, 1953, 2690, 4994
MlsI	TGGCCA	1 cut(s)	2285
MluNI	TGGCCA	1 cut(s)	2285
MlyI	GAGTC	6 cut(s)	647, 790, 2702, 3056, 3915, 5210
MmeI	TCCRAC	7 cut(s)	213, 818, 1313, 2422, 3152, 4993, 5354
Mox20I	TGGCCA	1 cut(s)	2285
Mph1103I	ATGCAT	2 cut(s)	1664, 5239
MroI	TCCGGA	2 cut(s)	174, 1358
MroNI	GCCGGC	1 cut(s)	4766
MroXI	GAANNNNTTC	3 cut(s)	1992, 3998, 4269
MscI	TGGCCA	1 cut(s)	2285
MslI	CAYNNNNRTG	4 cut(s)	1554, 2334, 3929, 4322
Msp20I	TGGCCA	1 cut(s)	2285
MspA1I	CMGCKG	1 cut(s)	618
MspCI	CTTAAG	1 cut(s)	3989
MspI	CCGG	5 cut(s)	9, 175, 1359, 3130, 4767
MspR9I	CCNGG	4 cut(s)	1474, 3130, 4014, 4407
MunI	CAATTG	4 cut(s)	2964, 3237, 4821, 4884
Mva1269I	GAATGC	1 cut(s)	2908
MvaI	CCWGG	3 cut(s)	1474, 4014, 4407
MvnI	CGCG	4 cut(s)	757, 886, 1109, 5263
NaeI	GCCGGC	1 cut(s)	4768
NciI	CCSGG	1 cut(s)	3130
NdeI	CATATG	2 cut(s)	991, 3820
NgoMIV	GCCGGC	1 cut(s)	4766
NlaIV	GGNNCC	9 cut(s)	7, 403, 1174, 1175, 2163, 3264, 4357, 4881, 5209
NmeAIII	GCCGAG	1 cut(s)	5156
NmuCI	GTSAC	8 cut(s)	62, 73, 1061, 1556, 2094, 2599, 3475, 4294
NruI	TCGCGA	1 cut(s)	5263
NsiI	ATGCAT	2 cut(s)	1664, 5239
NspI	RCATGY	3 cut(s)	3410, 4424, 4766
NspV	TTCGAA	2 cut(s)	1143, 2490
OliI	CACNNNNGTG	1 cut(s)	4322
PaeI	GCATGC	3 cut(s)	3410, 4424, 4766
PagI	TCATGA	2 cut(s)	417, 2716
PaqCI	CACCTGC	1 cut(s)	86
PceI	AGGCCT	1 cut(s)	4088
PciSI	GCTCTTC	3 cut(s)	1770, 2800, 4056
PctI	GAATGC	1 cut(s)	2908
PdiI	GCCGGC	1 cut(s)	4768
PdmI	GAANNNNTTC	3 cut(s)	1992, 3998, 4269
PflFI	GACNNNGTC	1 cut(s)	292
PfoI	TCCNGGA	2 cut(s)	4012, 4405
PleI	GAGTC	6 cut(s)	647, 790, 2701, 3055, 3915, 5210
PmaCI	CACGTG	1 cut(s)	137
PmlI	CACGTG	1 cut(s)	137
PpsI	GAGTC	6 cut(s)	647, 790, 2701, 3055, 3915, 5210
Ppu21I	YACGTR	2 cut(s)	137, 3089
PpuMI	RGGWCCY	3 cut(s)	470, 1173, 3263
PshAI	GACNNNNGTC	1 cut(s)	3803
PshBI	ATTAAT	1 cut(s)	2192
PsiI	TTATAA	4 cut(s)	662, 2132, 2769, 3959
Psp5II	RGGWCCY	3 cut(s)	470, 1173, 3263
Psp6I	CCWGG	3 cut(s)	1472, 4012, 4405
PspCI	CACGTG	1 cut(s)	137
PspFI	CCCAGC	3 cut(s)	675, 4502, 5043
PspGI	CCWGG	3 cut(s)	1472, 4012, 4405
PspN4I	GGNNCC	9 cut(s)	7, 403, 1174, 1175, 2163, 3264, 4357, 4881, 5209
PspPPI	RGGWCCY	3 cut(s)	470, 1173, 3263
PstI	CTGCAG	3 cut(s)	623, 2992, 3562
PstNI	CAGNNNCTG	3 cut(s)	370, 3399, 4292
PsuI	RGATCY	3 cut(s)	2161, 4837, 4879
PsyI	GACNNNGTC	1 cut(s)	292
PvuII	CAGCTG	1 cut(s)	618
RruI	TCGCGA	1 cut(s)	5263
RseI	CAYNNNNRTG	4 cut(s)	1554, 2334, 3929, 4322
SalI	GTCGAC	1 cut(s)	3796
SapI	GCTCTTC	3 cut(s)	1770, 2800, 4056
SchI	GAGTC	6 cut(s)	647, 790, 2702, 3056, 3915, 5210
ScrFI	CCNGG	4 cut(s)	1474, 3130, 4014, 4407
SduI	GDGCHC	4 cut(s)	1318, 1953, 2690, 4994
SfcI	CTRYAG	7 cut(s)	102, 619, 2829, 2988, 3558, 3612, 4845
SfuI	TTCGAA	2 cut(s)	1143, 2490
SmiMI	CAYNNNNRTG	4 cut(s)	1554, 2334, 3929, 4322
SmlI	CTYRAG	9 cut(s)	23, 3020, 3026, 3989, 4208, 4673, 4980, 5075, 5506
SmoI	CTYRAG	9 cut(s)	23, 3020, 3026, 3989, 4208, 4673, 4980, 5075, 5506
SpeI	ACTAGT	1 cut(s)	2150
SphI	GCATGC	3 cut(s)	3410, 4424, 4766
SseBI	AGGCCT	1 cut(s)	4088
SspI	AATATT	4 cut(s)	1516, 1864, 1962, 4652
StuI	AGGCCT	1 cut(s)	4088
StyD4I	CCNGG	4 cut(s)	1472, 3128, 4012, 4405
StyI	CCWWGG	3 cut(s)	749, 4596, 5590
TaiI	ACGT	7 cut(s)	139, 309, 2429, 3091, 3628, 3860, 4060
TaqII	GACCGA	1 cut(s)	4154
TatI	WGTACW	3 cut(s)	850, 1410, 5604
TauI	GCSGC	1 cut(s)	5320
TseFI	GTSAC	8 cut(s)	62, 73, 1061, 1556, 2094, 2599, 3475, 4294
Tsp45I	GTSAC	8 cut(s)	62, 73, 1061, 1556, 2094, 2599, 3475, 4294
TspGWI	ACGGA	6 cut(s)	756, 767, 1903, 4047, 4565, 5590
Tth111I	GACNNNGTC	1 cut(s)	292
Vha464I	CTTAAG	1 cut(s)	3989
VneI	GTGCAC	1 cut(s)	4990
VspI	ATTAAT	1 cut(s)	2192
XagI	CCTNNNNNAGG	3 cut(s)	1436, 1833, 4310
XapI	RAATTY	5 cut(s)	57, 320, 1366, 3287, 3979
XbaI	TCTAGA	1 cut(s)	344
XceI	RCATGY	3 cut(s)	3410, 4424, 4766
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2383, 3326
XmiI	GTMKAC	1 cut(s)	3797
XmnI	GAANNNNTTC	3 cut(s)	1992, 3998, 4269
Zsp2I	ATGCAT	2 cut(s)	1664, 5239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.