Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005424

Physical Location & Seq

Genomic Coordinates Reverse (-)

9687125 .. 9709169

Gene Structure 22045 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1079400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5670 bp

ATGGGGACCGGACGGAGGCAGTTGAAGATTATGCTATGGAAGAATTGGCTTCTCAAAGTTCGTCACCCTTTCGTCACCTGCGCTGAGATATTACTTCCTACAGTGGTGATGTTGTTGTTAATAGCTGTGAGGATGCATGTTGATACACAGATTCATCCAGCGCAGCCGTATATCCGGAATGGGATGTTTGTGGAAGTGGGCAAAGGAGTGTCACCTAATTTTGAACAAGTATTGGAATTACTGTTGAATAAGGAAGAGTTTCTGGCTTTTGCACCAGATACAGAAGAAACAAGGTCAATGATTAACATAATGTCGGTTAAATTTCCCCTCTTGAAGCGTGTTTCTAGAGTTTACAAAGATGAACAGGAGCTGGAAACCTACATACGCTCAGATCTCTATGGTACCTGCAATCAAATCTTGAATTGCTTGAATCCAAAAATCAAAGGAGCAGTTGTATTTCATGATCAAGGTCCTCAATCATTTGATTATAGCATACGTCTTAATCACACATGGGCTTTCTCGGGATTTCCTGATGTCAAATCCATCATGGACACGAATGGCCCCTACCTCAATGACTTGGAACTTGGCGTAAATGCTGTTCCGACCATGCAATACAGCGCTAGTGGATTTTTAACTCTCCAGCAAGTACTGGATTCATTTATTATTTTTGCGGCCCAACAATCTGACACCAAGGATATAGAACTTCCTTCATCGCTGTCCTTTGGTGAACCTTCCTTTCTAAACGTTCCATGGATGCATTACAGCCCATCAAACATTCGAATAGTCCCGTTTCCAACTCGTGAATACACTGATGATGAGTTCCAGTCCATCATAAAAAGTGTGATGGGTGTATTGTATCTTCTGGGATTCCTCTACCCAATTTCTCGGTTGATCAGCTATTCGGTGTTTGAGAAGGAACAGAAGATAAAAGAAGGTCTTTATATGATGGGTCTAAAAGACGGGATATTCCATCTCTCATGGTTCATTACATATGCTTTGCAGTTCGCTATTTCTTCTGCAATCATTACAGTTTCCACTATGGGTAACCTTTTCAAATATAGTGATAAGTCAGTTGTGTTCATTTACTTCTTCTTCTTCGGACTGAGCGCGATTATGCTGTCATTTTTAATCTCGACATGCTTCACACGGGCAAAGACAGCTGTTGCTGTTGGGACCCTTGCTTTTCTGGGTGCTTTTTTCCCTTACTACTCTGTCAATGATGAGGCTGTCCCTATGATATTAAAGGTTGTTGCTTCTCTGCTTTCGCCCACGGCATTTGCTCTAGGGTCAATTAACTTTGCTGATTATGAGCGTGCTCATGTTGGCCTTCGTTGGAGCAACATATGGCGGGCATCATCTGGAGTGAATTTTCTGGTCTGTCTACTGATGATGTTGCTCGATGCATTGCTCTACTGTTTAATTGGTCTTTATCTGGACAAGGTCCTTCCGAGGGAGAATGGAATTAGATATCCATGGAACTTCATTTTTCAGAAGTGCTTCTGGAAAAATCCAAGCATTAAGGAACTTAATAATCACAATTCCAGTCTAGAAGTTAATAGCCATGACAAGGACTGCAAGAAGGCAAGTTTTTCTGGGAAAGATAATGCCAGAGCATCTGTGGAAGCGATAACCTTTGACATGAAACAACAAGAGCTTGACCATAGATGCATCCAAATAAGGAATCTGCATAAGGTTTATGCCAGTAAAAAGGGGAAATGCTGTGCTGTTAACTCATTAGAGCTCACCATGTATGAAAATCAGATTCTTGCTCTTCTAGGACACAACGGGGCTGGTAAAAGCACAACGATATCAATGCTTGTTGGCCTTCTTCGCCCTACTTCAGGGGATGCTTTGGTTTTCGGGAAGAATATTGTTACAGACATGGATGAGATACGAAAGGAATTGGGGGTGTGTCCTCAAAGTGATATCCTTTTTCCAGAATTGACAGTGAGGGAACATTTGGAAATATTTGCTATACTGAAGGGTGTGCAGGAAGATCTTCTGAACGGCGCTGTTGTTGATATGGTTGATCAAGTAGGTTTGGCTGATAAAATGAACACTGCTGTGAGGGCTCTATCTGGAGGCATGAAGAGGAAGTTGTCTCTTGGAATTGCACTGATAGGAAACAGTAAGGTTATAATTTTGGATGAACCTACTAGTGGAATGGATCCATACTCCATGCGCTTGACATGGCAGCTAATAAAAAAAATCAGAAAGGGAAGGATAGTGTTACTGACAACGCACTCAATGGATGAAGCTGAAGTACTTGGAGATCGTATAGCCATCATGGCTAATGGTTCCTTGAAATGTTGCGGAAGTTCCCTTTTCTTGAAGCGTCAGTACGGGGTTGGTTATACTTTAACCTTGGTGAAATCGACACCTACTGCCTGTGTAGCTTCTGACATTGTTTACCGCCATATCCCATCAGCAACATGTGTGAGCGAGGTAGGAACGGAGATTTCCTTTAAGCTTCCTTTGGCATCCGCATCATCCTTTGAATGTATGTTTAGGGAAATTGAGAATTGCATGAAACGATCTACATCAAACTCAGAAACAAGTTCTGGCGAGGAGAAGGACTATTTGGGCATTGAAAGTTATGGAATATCTGTAACAACTTTGGAGGAGGTATTCTTGAGGGTTGCAGGATGCGACTATGCTGAAGCTGCTAGTTTTGAACAGAAGACTGGTCAACAGTGTCTTGATTCTTTGATTTCTCAGTCTTCTCATGATTCTTCTCCCAAAAAGATCTCTGAGTCAAAAAAATCCTTTGGATATTATAAAGAGATTCTTGGGTTCTTATTTAGAATAGTGGGAAGAGCCTGTGGTTTGGTTGTCGCTACAGTTTTAAGTTTTTTAAACTTTGTTGGTGGTCATTGCTGTAGCTGTTGTTTCATTTCAAGGTCAACTTTTTGGCGACATTCTAAAGCATTGCTCACAAAGAGGGCAATATCTGCTCGTAGAGACCGGAAAACAATTGTTTTCCAGCTTGTTATTCCTGCAGTTTTCTTATTTTTCGGTCTTCTGTTTCTCAAGCTCAAGCCACATCCTGATCAGCAGTCTGTTACATTTACAACTTCACATTTTAATCCACTATTACGAGGTGGTGGTGGTGGTGGCCCAATCCCTTTTAATCTGTCCTTGCCTATTGCAAAGGAGGTTGCACAGTATGTTAAAGGGGGTTGGATACAAGAATTCAGACCTAGTGCATATAGATTCCCTAATTCAGATAAGATATTGGATGATGCAGTTGAGGCAGCTGGGCCAACCATGGGACCTGTTTTACTTTCAATTAGCGAATTTTTAATGTCTAGCTTTAACGAATCTTACCAGTCAAGGTATGGGGCAATTTTGATGGATGATCAGAATGATGATGGAAGTTTAGGGTACACTGTGCTACACAACAGTTCCTGTCAGCATGCTGCCCCTACCTTTATCAATTTAATGAATGCTGCAATTCTTAGGCTTGCTGCTCGTAATAAGAATATGACAATCCAAACGCGTAATCACCCTCTCCCAATGACAAATAGCCAACATCTGCAACGTCATGATTTGGATGCATTCTCAGCTGCAGTTATTGTTAGTATTGCATTTTCATTCATCCCTGCATCATTTGCCGTTCCTATTGTGAAGGAGCGTGAAGTAAAAGCCAAACACCAACAACTGATTAGTGGGGTTTCTATTCTTTCATATTGGGCTTCTACTTTCATATGGGACTTCATCAGCTTCCTTTTTCCTTCATTGTTTGCAATAATTCTTTTTTACATCTTTGGTCTGGAGCAGTTCATTGGAAGCGGATATTTATTGTCAACAGTTGTCATGTTTTTAGCGTATGGATTAGCAATCGCATCATCCACATATTGCCTTACATTTTTCTTCTCTGACCACAGTATGGCTCAGAATGTGGTTCTCTTGGTCCACTTCTTTACGGGACTCATTCTTATGGTTATCTCATTCATCATGGGACTTATAAAGACAACAGCAAGTGCAAATTCTTTTCTTAAGAACTTTTTCAGATTATCTCCAGGGTTTTGCTTTGCTGATGGACTTGCATCACTGGCTCTTCTCCGGCAAGATATGAAAGATAAATCCAGCAATCAGGCCCTTGATTGGAACGTTACTGGTGGCTCAATTTGTTATCTTGGTATTGAGAGCATTTGTTACTTCCTTTTGACACTTGGGCTTGAACTCTTACTTTCTAACAAGTGGACACTAGCCACACTGAAGGAATGTTGGAACAACATTAGAAGTATCGAACATGGCACCCCAAGTTATCTAGAACCCCTTCTGAAGAGTTCCTCAGATGTTACTCTTGACCTTGATGAAGATATCGATGTAAAGACAGAAAGAACTAGGGTGCTTTCAGGTTCCATTGACAATGCCATAATTTATTTGCGGAATCTTTGGAAGGTGTTTCCAGGAGGAAAGCATCATAGTGCAAAAATTGCGGTACATTCATTGACTTTCTCAGTTCAGGAAGGGGAGTGTTTTGGTTTTTTAGGAACAAATGGAGCTGGAAAGACCACAACTCTGTCAATGCTGACTGGAGAGGAATCTCCAACTGATGGGACTGCTTATATATTTGGAAGGGATATATGCTCTAATCCAAAGGCTGCTCGTCGACATATTGGATTTTGCCCACAGTTTGATGCTTTGTTGGAATTCTTAACTGTTAAAGAGCATCTTGAGCTTTATGCCACAATAAAAGGAGTACCAGATCACCGGTTGGATGATGTTGTAATGGAAAAGTTAATGGAGTTTGACTTGTTAAAGCATGCCAACAAACCATCATTTTCTCTCAGTGGGGGAAACAAGCGTAAATTATCTGTCGCAATTGCGATGATTGGAGATCCTCCTATAGTCATTCTTGATGAACCGTCAACAGGTATGGATCCAATTGCCAAAAGATTCATGTGGGAAGTGATATCTCGTCTATCAACCAGACGAGGAAAGACAGCAGTAATACTTACTACGCATAGCATGAATGAAGCTCAAGCACTTTGCACTCGTATGGGGATCATGGTCGGAGGTCGATTAAGATGCATTGGTAGTCCCCAGCATTTGAAAACTCGATTTGGAAATCATCTTGAGCTAGAGGTTAAACCTTTTGAAGTCAGCTCTGTGGACTTACAAAATCTGTGCCGAGTTATTCAAGAATGGCTGTCATCTGTTCCATCCCATCCCAGGAGCTTACTTGATGGCCTTGAAATATGCATTGGGGCAGACTCCATTCTGGCTGAAAATGCCACAGTGGCAGAGATTAGTTTGTCGCGAGAAATGATAATTATGATTGGACGATGGCTTGGCAATGAAGAAAGAATAAAGACACTAATATCTCCGCTGCCAATTTCTGATGGGGTGATTGGTGAACAGTTAATTGAGCAGTTGGTTCGAGATGGGGGTATACCATTGCCAATATTTTCTGAGTGGTGGTTGTCCAATGAGAAGTTTTCAGCAATTGATTCATTTGTCCTCACATCATTCCCTGGTGCAATATTCCAGGGGTTTAATGGCTTGAGTGCTAAATACCAGTTGCCATATGGACAGGGCCTCTCGCTTGCAGATGTCTTTGGGCACCTTGAACGGAGCAGACATCAACTTGGCATTGCGGAATACAGTATTAGCCAGTCCACTCTTGAAACTATTTTCAACCATTTTGCGGCTAACTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006869 GO:0008150 GO:0010876 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0033036 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko02010 map02010

Pfam Domains

Protein Families

ABC2_membrane_3 ABC_tran

Protein Analysis

1890

Amino Acids

210.29

Weight (kDa)

7.12

Isoelectric Point (pI)

38.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC2_membrane_3	PF12698	191 - 475	1.7e-20	ABC-2 family transporter protein
ABC_tran	PF00005	577 - 720	7.8e-31	ABC transporter
ABC2_membrane_3	PF12698	990 - 1392	7.7e-40	ABC-2 family transporter protein
ABC_tran	PF00005	1484 - 1626	6.4e-27	ABC transporter
AAA_21	PF13304	1560 - 1661	2.4e-09	AAA domain, putative AbiEii toxin, Type IV TA system
R1_ABCA1	PF23321	1813 - 1875	2.6e-10	ABCA1-like, C-terminal R1 regulatory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0005424)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41700 AT2G41700
fragaria_vesca	FvH4_6g24100 FvH4_6g24100
malus_domestica	MD12G1079400.v1.1 MD14G1075700.v1.1
prunus_persica	Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1
pyrus_communis	pycom12g06660
rosa_chinensis	RchiOBHm_Chr3g0482761
rosa_laevigata	RLG00000023331
rosa_multiflora	Rmu_co8213162.1_g000001 Rmu_sc0001542.1_g000002 Rmu_sc0021223.1_g000001
rosa_roxburghii	Rroxscaffold_6G00398990
rosa_rugosa	Rorug03G0198800 Rorug03G0198900
rosa_samantha	Rh3AG249700 Rh3BG285700 Rh3CG283300 Rh3DG279300
rosa_wichuraiana	Rw3G022560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	2138, 2778, 3968
AarI	CACCTGC	1 cut(s)	86
Acc36I	ACCTGC	2 cut(s)	86, 413
Acc65I	GGTACC	1 cut(s)	401
AccB1I	GGYRCC	3 cut(s)	401, 4259, 5571
AccB7I	CCANNNNNTGG	2 cut(s)	3889, 4562
AccI	GTMKAC	3 cut(s)	1381, 4618, 5401
AccII	CGCG	3 cut(s)	1109, 3499, 5269
AccIII	TCCGGA	1 cut(s)	174
AclI	AACGTT	2 cut(s)	744, 4113
AclWI	GGATC	6 cut(s)	2162, 2175, 4841, 4883, 4896, 5021
AcsI	RAATTY	6 cut(s)	320, 1366, 3191, 3296, 3988, 4658
AcuI	CTGAAG	6 cut(s)	1824, 2000, 2280, 2679, 4241, 4307
AfaI	GTAC	7 cut(s)	403, 648, 2265, 2342, 3386, 4449, 4710
AfeI	AGCGCT	1 cut(s)	619
AflII	CTTAAG	1 cut(s)	3998
AflIII	ACRYGT	2 cut(s)	2432, 3497
AgeI	ACCGGT	1 cut(s)	4719
AhlI	ACTAGT	1 cut(s)	2156
AjnI	CCWGG	5 cut(s)	4021, 4414, 5180, 5482, 5496
AjuI	GAANNNNNNNTTGG	4 cut(s)	2564, 2596, 5196, 5228
AleI	CACNNNNGTG	1 cut(s)	2063
Alw21I	GWGCWC	2 cut(s)	1318, 1743
Alw26I	GTCTC	2 cut(s)	2106, 2955
AlwI	GGATC	6 cut(s)	2162, 2175, 4841, 4883, 4896, 5021
AlwNI	CAGNNNCTG	2 cut(s)	370, 3408
Ama87I	CYCGRG	1 cut(s)	520
Aor13HI	TCCGGA	1 cut(s)	174
Aor51HI	AGCGCT	1 cut(s)	619
AoxI	GGCC	9 cut(s)	559, 672, 1324, 1822, 3115, 3260, 4098, 5197, 5545
ApoI	RAATTY	6 cut(s)	320, 1366, 3191, 3296, 3988, 4658
AsiGI	ACCGGT	1 cut(s)	4719
Asp700I	GAANNNNTTC	5 cut(s)	258, 1496, 1998, 4007, 4281
Asp718I	GGTACC	1 cut(s)	401
AspLEI	GCGC	6 cut(s)	83, 163, 620, 1109, 2012, 2184
AsuII	TTCGAA	1 cut(s)	778
AvaI	CYCGRG	1 cut(s)	520
AvaII	GGWCC	6 cut(s)	6, 470, 1173, 1441, 3272, 3913
BaeGI	GKGCMC	1 cut(s)	5574
BamHI	GGATCC	2 cut(s)	2167, 4888
BanI	GGYRCC	3 cut(s)	401, 4259, 5571
BanII	GRGCYC	2 cut(s)	1743, 2074
BarI	GAAGNNNNNNTAC	6 cut(s)	843, 875, 2247, 2279, 2324, 2356
BauI	CACGAG	2 cut(s)	798, 5665
BbsI	GAAGAC	3 cut(s)	2687, 2712, 3011
Bbv12I	GWGCWC	2 cut(s)	1318, 1743
BceAI	ACGGC	4 cut(s)	151, 1287, 2023, 3599
BcgI	CGANNNNNNTGC	6 cut(s)	1795, 1829, 5019, 5053, 5369, 5403
BciT130I	CCWGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
BciVI	GTATCC	1 cut(s)	3177
BclI	TGATCA	5 cut(s)	463, 891, 2029, 3049, 3358
BcoDI	GTCTC	2 cut(s)	2106, 2955
BcuI	ACTAGT	1 cut(s)	2156
BfmI	CTRYAG	6 cut(s)	99, 2838, 2878, 2997, 3567, 4854
BfoI	RGCGCY	2 cut(s)	621, 2013
BfrI	CTTAAG	1 cut(s)	3998
BfuAI	ACCTGC	2 cut(s)	86, 413
BfuI	GTATCC	1 cut(s)	3177
BglI	GCCNNNNNGGC	2 cut(s)	2288, 5249
BglII	AGATCT	3 cut(s)	391, 1996, 2745
BmcAI	AGTACT	2 cut(s)	648, 2265
Bme1390I	CCNGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
Bme18I	GGWCC	6 cut(s)	6, 470, 1173, 1441, 3272, 3913
BmeT110I	CYCGRG	1 cut(s)	520
BmrFI	CCNGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
BpiI	GAAGAC	3 cut(s)	2687, 2712, 3011
BpmI	CTGGAG	6 cut(s)	623, 1380, 2100, 3794, 4005, 4563
Bpu14I	TTCGAA	1 cut(s)	778
BpuEI	CTTGAG	7 cut(s)	2653, 3014, 3020, 4703, 4974, 5105, 5533
Bsa29I	ATCGAT	1 cut(s)	4329
BsaBI	GATNNNNATC	1 cut(s)	2539
BsaI	GGTCTC	1 cut(s)	2955
BsaWI	WCCGGW	4 cut(s)	8, 174, 2964, 4719
BsaXI	ACNNNNNCTCC	2 cut(s)	438, 468
Bse118I	RCCGGY	1 cut(s)	4719
Bse3DI	GCAATG	6 cut(s)	1403, 2872, 2927, 5311, 5405, 5601
Bse8I	GATNNNNATC	1 cut(s)	2539
BseAI	TCCGGA	1 cut(s)	174
BseBI	CCWGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
BseCI	ATCGAT	1 cut(s)	4329
BseJI	GATNNNNATC	1 cut(s)	2539
BseMI	GCAATG	6 cut(s)	1403, 2872, 2927, 5311, 5405, 5601
BseRI	GAGGAG	2 cut(s)	2582, 2636
BseSI	GKGCMC	1 cut(s)	5574
BseYI	CCCAGC	2 cut(s)	3257, 5052
BsgI	GTGCAG	1 cut(s)	2009
Bsh1236I	CGCG	3 cut(s)	1109, 3499, 5269
BshFI	GGCC	9 cut(s)	561, 674, 1326, 1824, 3117, 3262, 4100, 5199, 5547
BshNI	GGYRCC	3 cut(s)	401, 4259, 5571
BshTI	ACCGGT	1 cut(s)	4719
BshVI	ATCGAT	1 cut(s)	4329
BsiHKAI	GWGCWC	2 cut(s)	1318, 1743
BsiHKCI	CYCGRG	1 cut(s)	520
BsiSI	CCGG	5 cut(s)	9, 175, 2965, 4066, 4720
BsmAI	GTCTC	2 cut(s)	2106, 2955
BsmI	GAATGC	2 cut(s)	3451, 3557
BsnI	GGCC	9 cut(s)	561, 674, 1326, 1824, 3117, 3262, 4100, 5199, 5547
Bso31I	GGTCTC	1 cut(s)	2955
BsoBI	CYCGRG	1 cut(s)	520
Bsp119I	TTCGAA	1 cut(s)	778
Bsp1286I	GDGCHC	4 cut(s)	1318, 1743, 2074, 5574
Bsp13I	TCCGGA	1 cut(s)	174
Bsp19I	CCATGG	3 cut(s)	749, 1472, 3267
Bsp68I	TCGCGA	1 cut(s)	5269
BspANI	GGCC	9 cut(s)	561, 674, 1326, 1824, 3117, 3262, 4100, 5199, 5547
BspDI	ATCGAT	1 cut(s)	4329
BspEI	TCCGGA	1 cut(s)	174
BspFNI	CGCG	3 cut(s)	1109, 3499, 5269
BspHI	TCATGA	3 cut(s)	460, 2725, 3544
BspMAI	CTGCAG	2 cut(s)	3001, 3571
BspMI	ACCTGC	2 cut(s)	86, 413
BspPI	GGATC	6 cut(s)	2162, 2175, 4841, 4883, 4896, 5021
BspQI	GCTCTTC	3 cut(s)	1776, 2809, 4065
BspT104I	TTCGAA	1 cut(s)	778
BspT107I	GGYRCC	3 cut(s)	401, 4259, 5571
BspTI	CTTAAG	1 cut(s)	3998
BspTNI	GGTCTC	1 cut(s)	2955
BsrDI	GCAATG	6 cut(s)	1403, 2872, 2927, 5311, 5405, 5601
BsrFI	RCCGGY	1 cut(s)	4719
BssAI	RCCGGY	1 cut(s)	4719
BssNAI	GTATAC	1 cut(s)	5402
BssSI	CACGAG	2 cut(s)	798, 5665
BssT1I	CCWWGG	5 cut(s)	690, 749, 1472, 2364, 3267
Bst1107I	GTATAC	1 cut(s)	5402
Bst2BI	CACGAG	2 cut(s)	798, 5665
Bst2UI	CCWGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
Bst6I	CTCTTC	6 cut(s)	249, 1776, 2084, 2809, 4065, 4283
BstAFI	CTTAAG	1 cut(s)	3998
BstAPI	GCANNNNNTGC	3 cut(s)	2951, 3611, 4442
BstBI	TTCGAA	1 cut(s)	778
BstC8I	GCNNGC	6 cut(s)	1314, 1350, 3417, 3465, 4773, 5556
BstDSI	CCRYGG	4 cut(s)	749, 1269, 1472, 3267
BstEII	GGTNACC	1 cut(s)	1043
BstENI	CCTNNNNNAGG	1 cut(s)	1839
BstFNI	CGCG	3 cut(s)	1109, 3499, 5269
BstH2I	RGCGCY	2 cut(s)	621, 2013
BstHHI	GCGC	6 cut(s)	83, 163, 620, 1109, 2012, 2184
BstMAI	GTCTC	2 cut(s)	2106, 2955
BstNI	CCWGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
BstNSI	RCATGY	5 cut(s)	140, 1140, 2436, 3419, 4775
BstPI	GGTNACC	1 cut(s)	1043
BstSCI	CCNGG	5 cut(s)	4021, 4414, 5180, 5482, 5496
BstSFI	CTRYAG	6 cut(s)	99, 2838, 2878, 2997, 3567, 4854
BstSLI	GKGCMC	1 cut(s)	5574
BstUI	CGCG	3 cut(s)	1109, 3499, 5269
BstV2I	GAAGAC	3 cut(s)	2687, 2712, 3011
BstX2I	RGATCY	6 cut(s)	391, 1996, 2167, 2745, 4846, 4888
BstYI	RGATCY	6 cut(s)	391, 1996, 2167, 2745, 4846, 4888
BstZ17I	GTATAC	1 cut(s)	5402
Bsu15I	ATCGAT	1 cut(s)	4329
BsuI	GTATCC	1 cut(s)	3177
BsuRI	GGCC	9 cut(s)	561, 674, 1326, 1824, 3117, 3262, 4100, 5199, 5547
BsuTUI	ATCGAT	1 cut(s)	4329
BtgI	CCRYGG	4 cut(s)	749, 1269, 1472, 3267
BtgZI	GCGATG	2 cut(s)	696, 4850
BtsI	GCAGTG	1 cut(s)	2058
BtuMI	TCGCGA	1 cut(s)	5269
BveI	ACCTGC	2 cut(s)	86, 413
Cac8I	GCNNGC	6 cut(s)	1314, 1350, 3417, 3465, 4773, 5556
CaiI	CAGNNNCTG	2 cut(s)	370, 3408
CciI	TCATGA	3 cut(s)	460, 2725, 3544
CfoI	GCGC	6 cut(s)	83, 163, 620, 1109, 2012, 2184
Cfr10I	RCCGGY	1 cut(s)	4719
ClaI	ATCGAT	1 cut(s)	4329
CseI	GACGC	1 cut(s)	2324
Csp6I	GTAC	7 cut(s)	402, 647, 2264, 2341, 3385, 4448, 4709
CspAI	ACCGGT	1 cut(s)	4719
CspCI	CAANNNNNGTGG	2 cut(s)	1258, 1293
CviQI	GTAC	7 cut(s)	402, 647, 2264, 2341, 3385, 4448, 4709
DraI	TTTAAA	1 cut(s)	2856
Eam1104I	CTCTTC	6 cut(s)	249, 1776, 2084, 2809, 4065, 4283
EarI	CTCTTC	6 cut(s)	249, 1776, 2084, 2809, 4065, 4283
Ecl136II	GAGCTC	1 cut(s)	1741
Eco130I	CCWWGG	5 cut(s)	690, 749, 1472, 2364, 3267
Eco24I	GRGCYC	2 cut(s)	1743, 2074
Eco31I	GGTCTC	1 cut(s)	2955
Eco32I	GATATC	5 cut(s)	1469, 1809, 1927, 4327, 4923
Eco47I	GGWCC	6 cut(s)	6, 470, 1173, 1441, 3272, 3913
Eco47III	AGCGCT	1 cut(s)	619
Eco53kI	GAGCTC	1 cut(s)	1741
Eco57I	CTGAAG	6 cut(s)	1824, 2000, 2280, 2679, 4241, 4307
Eco88I	CYCGRG	1 cut(s)	520
Eco91I	GGTNACC	1 cut(s)	1043
EcoICRI	GAGCTC	1 cut(s)	1741
EcoNI	CCTNNNNNAGG	1 cut(s)	1839
EcoO109I	RGGNCCY	6 cut(s)	470, 1173, 1441, 3272, 4099, 5545
EcoO65I	GGTNACC	1 cut(s)	1043
EcoRI	GAATTC	2 cut(s)	3191, 4658
EcoRII	CCWGG	5 cut(s)	4021, 4414, 5180, 5482, 5496
EcoRV	GATATC	5 cut(s)	1469, 1809, 1927, 4327, 4923
EcoT14I	CCWWGG	5 cut(s)	690, 749, 1472, 2364, 3267
EcoT22I	ATGCAT	7 cut(s)	138, 759, 1405, 1670, 3559, 5042, 5213
EcoT38I	GRGCYC	2 cut(s)	1743, 2074
ErhI	CCWWGG	5 cut(s)	690, 749, 1472, 2364, 3267
FalI	AAGNNNNNCTT	8 cut(s)	921, 953, 2772, 2804, 2890, 2922, 4665, 4697
FauI	CCCGC	1 cut(s)	1341
FauNDI	CATATG	4 cut(s)	991, 1343, 3707, 5536
FbaI	TGATCA	5 cut(s)	463, 891, 2029, 3049, 3358
FblI	GTMKAC	3 cut(s)	1381, 4618, 5401
FriOI	GRGCYC	2 cut(s)	1743, 2074
GlaI	GCGC	6 cut(s)	82, 162, 619, 1108, 2011, 2183
GsaI	CCCAGC	2 cut(s)	3261, 5056
GsuI	CTGGAG	6 cut(s)	623, 1380, 2100, 3794, 4005, 4563
HaeII	RGCGCY	2 cut(s)	621, 2013
HaeIII	GGCC	9 cut(s)	561, 674, 1326, 1824, 3117, 3262, 4100, 5199, 5547
HapII	CCGG	5 cut(s)	9, 175, 2965, 4066, 4720
HgaI	GACGC	1 cut(s)	2324
HhaI	GCGC	6 cut(s)	83, 163, 620, 1109, 2012, 2184
Hin6I	GCGC	6 cut(s)	81, 161, 618, 1107, 2010, 2182
HinP1I	GCGC	6 cut(s)	81, 161, 618, 1107, 2010, 2182
HincII	GTYRAC	6 cut(s)	1729, 2690, 2904, 3807, 4619, 4878
HindII	GTYRAC	6 cut(s)	1729, 2690, 2904, 3807, 4619, 4878
HindIII	AAGCTT	1 cut(s)	2468
HpaI	GTTAAC	1 cut(s)	1729
HpaII	CCGG	5 cut(s)	9, 175, 2965, 4066, 4720
Hpy99I	CGWCG	1 cut(s)	4620
HpyCH4IV	ACGT	4 cut(s)	496, 744, 3541, 4113
HpySE526I	ACGT	4 cut(s)	496, 744, 3541, 4113
HspAI	GCGC	6 cut(s)	81, 161, 618, 1107, 2010, 2182
KflI	GGGWCCC	1 cut(s)	1173
Kpn2I	TCCGGA	1 cut(s)	174
KpnI	GGTACC	1 cut(s)	405
Ksp22I	TGATCA	5 cut(s)	463, 891, 2029, 3049, 3358
KspAI	GTTAAC	1 cut(s)	1729
LguI	GCTCTTC	3 cut(s)	1776, 2809, 4065
LmnI	GCTCC	8 cut(s)	367, 446, 1335, 3631, 3775, 4508, 5184, 5583
MaeII	ACGT	4 cut(s)	496, 744, 3541, 4113
MfeI	CAATTG	4 cut(s)	2973, 4830, 4893, 5454
MflI	RGATCY	6 cut(s)	391, 1996, 2167, 2745, 4846, 4888
MhlI	GDGCHC	4 cut(s)	1318, 1743, 2074, 5574
MluI	ACGCGT	1 cut(s)	3497
MlyI	GAGTC	3 cut(s)	2762, 3924, 5216
MmeI	TCCRAC	9 cut(s)	626, 818, 1313, 3161, 4211, 4580, 4635, 4704, 5002
Mph1103I	ATGCAT	7 cut(s)	138, 759, 1405, 1670, 3559, 5042, 5213
MroI	TCCGGA	1 cut(s)	174
MroXI	GAANNNNTTC	5 cut(s)	258, 1496, 1998, 4007, 4281
MslI	CAYNNNNRTG	4 cut(s)	2063, 3938, 4431, 5006
MspA1I	CMGCKG	4 cut(s)	1160, 3257, 3566, 5338
MspCI	CTTAAG	1 cut(s)	3998
MspI	CCGG	5 cut(s)	9, 175, 2965, 4066, 4720
MspR9I	CCNGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
MunI	CAATTG	4 cut(s)	2973, 4830, 4893, 5454
Mva1269I	GAATGC	2 cut(s)	3451, 3557
MvaI	CCWGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
MvnI	CGCG	3 cut(s)	1109, 3499, 5269
NcoI	CCATGG	3 cut(s)	749, 1472, 3267
NdeI	CATATG	4 cut(s)	991, 1343, 3707, 5536
NmeAIII	GCCGAG	1 cut(s)	5165
NmuCI	GTSAC	3 cut(s)	62, 73, 210
NruI	TCGCGA	1 cut(s)	5269
NsiI	ATGCAT	7 cut(s)	138, 759, 1405, 1670, 3559, 5042, 5213
NspI	RCATGY	5 cut(s)	140, 1140, 2436, 3419, 4775
NspV	TTCGAA	1 cut(s)	778
OliI	CACNNNNGTG	1 cut(s)	2063
PaeI	GCATGC	2 cut(s)	3419, 4775
PagI	TCATGA	3 cut(s)	460, 2725, 3544
PaqCI	CACCTGC	1 cut(s)	86
PciI	ACATGT	1 cut(s)	2432
PciSI	GCTCTTC	3 cut(s)	1776, 2809, 4065
PctI	GAATGC	2 cut(s)	3451, 3557
PdmI	GAANNNNTTC	5 cut(s)	258, 1496, 1998, 4007, 4281
PflFI	GACNNNGTC	1 cut(s)	1439
PflMI	CCANNNNNTGG	2 cut(s)	3889, 4562
PfoI	TCCNGGA	1 cut(s)	4414
PinAI	ACCGGT	1 cut(s)	4719
PleI	GAGTC	3 cut(s)	2761, 3924, 5216
PpsI	GAGTC	3 cut(s)	2761, 3924, 5216
PpuMI	RGGWCCY	4 cut(s)	470, 1173, 1441, 3272
PscI	ACATGT	1 cut(s)	2432
PsiI	TTATAA	3 cut(s)	2138, 2778, 3968
Psp124BI	GAGCTC	1 cut(s)	1743
Psp1406I	AACGTT	2 cut(s)	744, 4113
Psp5II	RGGWCCY	4 cut(s)	470, 1173, 1441, 3272
Psp6I	CCWGG	5 cut(s)	4021, 4414, 5180, 5482, 5496
PspEI	GGTNACC	1 cut(s)	1043
PspFI	CCCAGC	2 cut(s)	3257, 5052
PspGI	CCWGG	5 cut(s)	4021, 4414, 5180, 5482, 5496
PspPPI	RGGWCCY	4 cut(s)	470, 1173, 1441, 3272
PsrI	GAACNNNNNNTAC	2 cut(s)	582, 614
PstI	CTGCAG	2 cut(s)	3001, 3571
PstNI	CAGNNNCTG	2 cut(s)	370, 3408
PsuI	RGATCY	6 cut(s)	391, 1996, 2167, 2745, 4846, 4888
PsyI	GACNNNGTC	1 cut(s)	1439
PvuII	CAGCTG	3 cut(s)	1160, 3257, 3566
RruI	TCGCGA	1 cut(s)	5269
RsaI	GTAC	7 cut(s)	403, 648, 2265, 2342, 3386, 4449, 4710
RsaNI	GTAC	7 cut(s)	402, 647, 2264, 2341, 3385, 4448, 4709
RseI	CAYNNNNRTG	4 cut(s)	2063, 3938, 4431, 5006
SacI	GAGCTC	1 cut(s)	1743
SalI	GTCGAC	1 cut(s)	4617
SapI	GCTCTTC	3 cut(s)	1776, 2809, 4065
ScaI	AGTACT	2 cut(s)	648, 2265
SchI	GAGTC	3 cut(s)	2762, 3924, 5216
ScrFI	CCNGG	5 cut(s)	4023, 4416, 5182, 5484, 5498
SduI	GDGCHC	4 cut(s)	1318, 1743, 2074, 5574
SfcI	CTRYAG	6 cut(s)	99, 2838, 2878, 2997, 3567, 4854
SfuI	TTCGAA	1 cut(s)	778
SinI	GGWCC	6 cut(s)	6, 470, 1173, 1441, 3272, 3913
SmiMI	CAYNNNNRTG	4 cut(s)	2063, 3938, 4431, 5006
SmlI	CTYRAG	8 cut(s)	2632, 3029, 3035, 3998, 4682, 4989, 5084, 5512
SmoI	CTYRAG	8 cut(s)	2632, 3029, 3035, 3998, 4682, 4989, 5084, 5512
SpeI	ACTAGT	1 cut(s)	2156
SphI	GCATGC	2 cut(s)	3419, 4775
SspI	AATATT	4 cut(s)	1870, 1968, 5415, 5493
SstI	GAGCTC	1 cut(s)	1743
StyD4I	CCNGG	5 cut(s)	4021, 4414, 5180, 5482, 5496
StyI	CCWWGG	5 cut(s)	690, 749, 1472, 2364, 3267
TaiI	ACGT	4 cut(s)	499, 747, 3544, 4116
TaqII	GACCGA	1 cut(s)	3005
TatI	WGTACW	2 cut(s)	646, 2263
TauI	GCSGC	2 cut(s)	674, 5660
TseFI	GTSAC	3 cut(s)	62, 73, 210
Tsp45I	GTSAC	3 cut(s)	62, 73, 210
TspGWI	ACGGA	3 cut(s)	28, 2468, 5596
Tth111I	GACNNNGTC	1 cut(s)	1439
Van91I	CCANNNNNTGG	2 cut(s)	3889, 4562
Vha464I	CTTAAG	1 cut(s)	3998
VpaK11BI	GGWCC	6 cut(s)	6, 470, 1173, 1441, 3272, 3913
XagI	CCTNNNNNAGG	1 cut(s)	1839
XapI	RAATTY	6 cut(s)	320, 1366, 3191, 3296, 3988, 4658
XbaI	TCTAGA	3 cut(s)	344, 1546, 4273
XceI	RCATGY	5 cut(s)	140, 1140, 2436, 3419, 4775
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1615, 3335, 5504, 5534
XmiI	GTMKAC	3 cut(s)	1381, 4618, 5401
XmnI	GAANNNNTTC	5 cut(s)	258, 1496, 1998, 4007, 4281
ZrmI	AGTACT	2 cut(s)	648, 2265
Zsp2I	ATGCAT	7 cut(s)	138, 759, 1405, 1670, 3559, 5042, 5213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.