Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0005424

Physical Location & Seq

Genomic Coordinates Forward (+)

20879090 .. 20899920

Gene Structure 20831 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00398990.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5013 bp

ATGGGGACCGGAACGAGACAGCTGAAGGCTATGCTTCGCAAGAATTGGCTTCTCAAAATTCGTCACCCTTTCGTCACCTGCGCTGAGATATTACTCCCTACTGTAGTAATGTTGATGCTCATAGCTGTAAGGACACGTGTTGATACGCAGATTCACCCTTCCCAGCCATATATCCGGAAAGGGATGTTGGTGGAAGTGGGCAAAGGAATATCCCCTAATTTTGAGCAAGTGTTGGAATTACTATTGAAGAAGGAAGAGATTTTGGCTTTTGCACCAGACACAGAAGAGACAAGGTCAATGATCAACGTGATGTCGATGAAATTTCCTCTCTTGAAGCTCCAGCAAGCACTAGACTCTTTTATAATTTTTGCGGCCCAGCAATCGGATACCAGAAATATAGAACTTCCTACTTCGCTGTCCTCTAGCACACCTTCCTCTCTCAAAGTTCCATGGACGCGATATGGTCCGTCAAACATCCGTATAGTCCCGTTTCCAACTCGTGAATACACTGATGATGAGTTCCAGTCCATCATAAAAAGTGTCATGGGTGTACTGTATCTTTTGGGATTTCTCTATCCAATTTCGCGCTTGATCAGCTATTCTGTGTTTGAAAAGGAACAAAAGATAAGAGAAGGTCTTTATATGATGGGGCTGAAAGATGGGGTATTCCATCTATCGTGGTTCATTGCATATGCTTTGCAGTTCGCAGTTTCTTCTCTTATTATTACAGTTTGCACGATGGATAGCCTTTTCAAGTATAGTGACAAGTCAGTGGTGTTCATTTACTTCTTCTTTTTCGGAATAAGCGCGATTATGCTGTCATTTTTAATCTCCACATTCTTCGAACGAGCGAAGACAGCGGTTGCGGTTGGGACCCTTGCTTTCTTGGGTGCTTTTTTCCCATACTACTCTGTCAATGACGAGGCTGTTCCTATGATTCTAAAGGTCATTGCCTCTCTGCTTTCACCTACGGCTTTTGCTCTGGGGTCAATCAACTTTGCTGATTATGAGCGTGCTCATGTTGGCCTTCGTTGGAGCAATATATGGCGGGCATCATCCGGAGTGAATTTTTCAGTCTGTCTCCTGATGATGTTGCTTGATGCGTTGCTGTACTGTGTAATTGGTCTTTACCTAGATAAGGTACTTCCGAGAGAAAATGGAGTTAGGTATCCCTGGAACTTCATTTTTCAGAAGTGCTTTTGGAAGAATCCAAATATTAACAAACACCACAACTCCAGTCCAGAAGTTCATATTCGTGACAAGGTATCCCAGAAGGCAATGTTTTCTCGGAAAGAAAATGCTAAAGCTGCTGTGGAAGCAATAACCTTCGATATGAGACAACAAGAGCTTGACCATAGATGCATCCAAATAAGGAATCTGCATAAGTTTTATGCTAGTAAAAAGGGGAAATGTTGTGCTGTCAACTCATTACAGCTCACCATGTATGAAAATCAGATTCTTGCTCTTCTAGGACATAATGGAGCTGGTAAAAGCACAACGATATCAATGCTTGTTGGCCTTCTTCGTCCTACTTCAGGGGATGCTATGGTGTTTGGAAAAAATATTATGACAGACATGGAGGAGATACGGAAAGAGTTGGGTGTATGCCCTCAGCATGATATCCTTTTTCCAGAACTGACGGTGAAGGAGCACTTGGAAATATTTGCTATATTGAAAGGTGTGCAGGAAGATTTTCTGAACAGCGTTGTTATTGATATGGTTGACCAAGTAGGGTTGGCCGATAAAATCAACACTGCTGTTATGGCTCTTTCTGGAGGAATGAAGAGGAAGTTGTCTCTTGGAATTGCACTAATTGGAAATAGTAAGGTTATAATTCTTGATGAACCTACTAGTGGAATGGATCCATACTCTATGCGCTTGACTTGGCAATTAATAAAAAAGATTAGAAAGGGCAGGATAGTATTACTGACAACACACTCAATGGATGAAGCTGAGGCACTTGGAGATCGCATAGCCATCATGGCCAATGGTTCTTTGAAATGCTGCGGAAGTTCCCTTTTCTTGAAGCATCAGTATGGGGTTGGTTATACTCTAACTCTAGTGAAGTCTGCACCCACTGCCTCTATGGCTGCTGATATTGTTTACCGCCATATCCCATCAGCAACATGTGTGAGTGAGGTTGGAACTGAGATTTCCTTTAAGCTTCCATTGGCATCCTCAACATCCTTCGAAAGTATGTTTAGAGAAATTGAGAGTTGCATGGGATCATCCACATCAAACTTAGAAACAAGTTGTGATGAGAAGGACTATATTGGCATTGAAAGTTATGGCATATCTGTCACAACTTTGGAGGAGGTGTTCCTGAGGGTTGCAGGATGCGATTATGACGAAGCTGCTTGTTTTGACCAGAAGAATGGTCCACTGTGCCCTGAGTCTCTGATTTCTCAAACTTCTCATGATCCTACCCACAAACAGATTCTTCACTCGAAGAAATCTTTTGCTTATTATAAAGAGATTCTTGGGGTCTTATTTACAATGGTGGGAAGAGCCTGTGGTTTAATTTTTGCTACAGTGTTAAGTTTCTTAAACTTCTTAGGTGTGCAATGCTGTGGCTGTTGTATCATTTCAAGGTCAACATTTTGGAGGCATTCTAAAGCATTATTCATAAAGAGGGCAATATCTGCCCGCAGAGACCGTAAAACAATTGTTTTCCAGCTTGTAATTCCTGCAGTTTTCTTATTTTTTGGTCTTTTATTTCTCAAGCTCAAGCCACATCCTGATCAGGAGTCTGTTACCTTCACAACTTCACATTTTAATCCACTATTACGTGGTGGTGGTGGTGGTGGTCCAATTCCTTTTGATCTATCCCGGCCTATTGCACAGGAGGTTGCAGAATACATAGAAGGGGGTTGGATACAAACCTTCAAACCTAGTGGGTATAAATTCCCCAACTCAGAGAAGGCATTGAATGATGCAATTGAGGTAGCAGGGGAAACTCTGGGACCTGCTTTACTTTCAATGAGTGAATTTTTGATGTCCAGCTTTAATGAATCTTACCAGTCAAGGTATGGCGCAGTTGTGATGGATGATCAGAGTGATGATGGGAGTTTAGGGTACACTGTGCTACACAACAGTTCCTGTCAGCATGCTGCCCCAACCTTTATCAATTTAGTAAATGCTGCAATTCTTCGGCTTGCTTCTCGTAATAAGAATGTGACAATTCAAACACGCAATCACCCTTTGCCAATGACAAAAAGTCAACTTCTGCAACGGCATGATTTGGATGCTTTCTCTGCTGCAGTTATTGTTAGTATCGCCTTCTCCTTCATTCCTGCGTCATTTGCTGTTCCTATAGTGAAGGAACGTGAAGTAAAAGCTAAACACCAACAACTTATTAGTGGAGTTTCCATACTGTCATATTGGACTTCTACATACATATGGGACTTCATCAGCTTCCTCTTTCCTTCATCTTTTGCCATAGTTCTTTTTTACATATTTGGTCTGGACCAGTTTATTGGAAGGGGATGTTTACTGTCGACTGTCGTCATGTTTTTAGCATACGGATTAGCAATTGCATCATCAACATACTGCCTTACGTTTTTCTTTTCTGATCACACTATGGCTCAGGTGGGATTATCTCCAGGATTTTGCTTTGCTGACGGACTTGCTTCACTGGCTCTTCTACGTCAAGATATGAAAAATAAAAGCAATAAGGCCTTTGATTGGAATGTTACTGGTGGCTCAATCTGCTACCTTTGTATTGAGAGCATTTGTTACTTCCTTTTGGCACTTGGTCTTGAAATCTTGCCTTTCAATAAATTGACACTAGCCACACTCAAGGAGTGGTGGAAGAGTATTAAAAGTATCCCACCTAGCACTTCAAGTTATCGAGAGCCCCTTCTGACTTCTTCCGCAGAATCTGTCACTCTGGACCTTGATGAGGACACAGATGTGAAGGCAGAAAGAACTAGGGTACTTTCCGGTTCCATTGACAATGCCATCATCTACTTGCGTAATCTTCGTAAGGTGTATCCAGGAGGACAGCAGCATGCTACAAAGGTTGCGGTACATTCATTGACTTTTTCAGTTCAAGAAGGGGAATGTTTCGGTTTTTTAGGAACAAATGGAGCTGGGAAGACCACAACTCTGTCTATGTTGACTGGTGAGGAATCTCCTACGGATGGGACTGCTTGTATATTTGGCAAGGATATTTCCTCCAATCCCAAGGCTGCTCGTCAACATATTGGGTTTTGCCCACAGTTTGATGCTCTGTTAGAATATTTAACTGTTCAAGAGCATCTTGAGCTTTATGCCACGATAAAAGGAGTACCAGATAATAAGATAGCTGAGGTTGTAATGGAAAAGTTGGTGGAGTTTGACTTGTTAAAGCATGCCGGCAAACCATCATTTTGCCTCAGTGGGGGAAACAAGCGAAAATTATCTGTCGCAATTGCTATGATTGGAGATCCTCCTATAGTTATTCTTGATGAACCATCAACAGGTATGGATCCAATTGCCAAAAGATTCATGTGGGAAGTGATATCTCGTTTATCGACCAGACGAGGAAAGACAGCAGTGATACTGACTACCCATAGTATGAATGAAGCTCAAGCTCTGTGCACCCGTATCGGAATCATGGTTGGAGGCCAATTACGGTGCATTGGAAGTCCCCAGCATTTAAAAACTCGATTTGGAAATCATCTTGAGCTAGAGGTTAAACCTATTGAAGTCAGCTCTGTGGACTTGGACAAGCTTTGCCGAGTTATCCAAGAAAGGCTCTCGTATGTTCCTTCTCATCCAATGAGCTTACTTGATGGCCTTGAAGTCTGCATTGGTGCCACTGACTCCATTGTGGCAGAAAATGCATCGGTGGCAGAGATTAGTTTATCACGAGAAATGATAATTATGATTGGACGATGGCTTGGGAATGAAGAAAGAATAAAGCCGCTTATATCTTCAACACCACTTTCAGATGGGGTGCTTGGTGAACAGTTATTTGAGCAGTTAGATCGAGATGGTATGAGAAAGTTTACTTTGAAATCTGTGCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006869 GO:0008150 GO:0010876 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0033036 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko02010 map02010

Pfam Domains

Protein Families

ABC2_membrane_3 ABC_tran

Protein Analysis

1670

Amino Acids

185.55

Weight (kDa)

8.06

Isoelectric Point (pI)

38.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC2_membrane_3	PF12698	154 - 376	2.2e-18	ABC-2 family transporter protein
ABC_tran	PF00005	475 - 618	1.1e-30	ABC transporter
ABC2_membrane_3	PF12698	887 - 1201	2.7e-28	ABC-2 family transporter protein
ABC_tran	PF00005	1344 - 1486	1.2e-27	ABC transporter
AAA_21	PF13304	1457 - 1521	6.7e-08	AAA domain, putative AbiEii toxin, Type IV TA system

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005424)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41700 AT2G41700
fragaria_vesca	FvH4_6g24100 FvH4_6g24100
malus_domestica	MD12G1079400.v1.1 MD14G1075700.v1.1
prunus_persica	Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1
pyrus_communis	pycom12g06660
rosa_chinensis	RchiOBHm_Chr3g0482761
rosa_laevigata	RLG00000023331
rosa_multiflora	Rmu_co8213162.1_g000001 Rmu_sc0001542.1_g000002 Rmu_sc0021223.1_g000001
rosa_roxburghii	Rroxscaffold_6G00398990
rosa_rugosa	Rorug03G0198800 Rorug03G0198900
rosa_samantha	Rh3AG249700 Rh3BG285700 Rh3CG283300 Rh3DG279300
rosa_wichuraiana	Rw3G022560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	362, 1832, 2469
AarI	CACCTGC	1 cut(s)	86
Acc36I	ACCTGC	2 cut(s)	86, 2974
AccB1I	GGYRCC	1 cut(s)	4796
AccI	GTMKAC	1 cut(s)	3497
AccII	CGCG	3 cut(s)	457, 586, 809
AccIII	TCCGGA	2 cut(s)	174, 1058
AclWI	GGATC	7 cut(s)	1856, 1869, 2233, 2414, 4421, 4463, 4476
AcoI	YGGCCR	2 cut(s)	1737, 1983
AcsI	RAATTY	5 cut(s)	57, 320, 1066, 2903, 2987
AcuI	CTGAAG	2 cut(s)	44, 1518
AcvI	CACGTG	1 cut(s)	137
AfaI	GTAC	7 cut(s)	552, 1112, 1143, 3077, 3936, 4029, 4290
AfiI	CCNNNNNNNGG	9 cut(s)	382, 1138, 1535, 1853, 3684, 3901, 4142, 4381, 4460
AflIII	ACRYGT	3 cut(s)	134, 136, 2126
AhdI	GACNNNNNGTC	1 cut(s)	3216
AhlI	ACTAGT	1 cut(s)	1850
AjnI	CCWGG	3 cut(s)	1172, 3601, 3994
AjuI	GAANNNNNNNTTGG	6 cut(s)	170, 202, 1637, 1669, 4776, 4808
AleI	CACNNNNGTG	1 cut(s)	3911
AloI	GAACNNNNNNTCC	2 cut(s)	2303, 2335
Alw21I	GWGCWC	3 cut(s)	1018, 1653, 4583
Alw26I	GTCTC	7 cut(s)	10, 281, 1085, 1330, 1800, 2400, 2646
Alw44I	GTGCAC	1 cut(s)	4579
AlwI	GGATC	7 cut(s)	1856, 1869, 2233, 2414, 4421, 4463, 4476
AlwNI	CAGNNNCTG	2 cut(s)	3099, 3881
Aor13HI	TCCGGA	2 cut(s)	174, 1058
AoxI	GGCC	9 cut(s)	372, 1024, 1516, 1737, 1983, 2831, 3675, 4606, 4777
ApaLI	GTGCAC	1 cut(s)	4579
ApeKI	GCWGC	9 cut(s)	1307, 2004, 2090, 2354, 3110, 3140, 3257, 4006, 4190
ApoI	RAATTY	5 cut(s)	57, 320, 1066, 2903, 2987
AseI	ATTAAT	1 cut(s)	1892
Asp700I	GAANNNNTTC	1 cut(s)	1692
AspLEI	GCGC	5 cut(s)	83, 588, 809, 1878, 3035
AspS9I	GGNCC	9 cut(s)	6, 373, 464, 873, 2378, 2807, 2963, 3466, 3892
AsuC2I	CCSGG	1 cut(s)	2830
AsuHPI	GGTGA	9 cut(s)	56, 67, 146, 957, 1429, 1654, 3188, 4136, 4958
AsuII	TTCGAA	2 cut(s)	843, 2190
AvaII	GGWCC	8 cut(s)	6, 464, 873, 2378, 2807, 2963, 3466, 3892
AxyI	CCTNAGG	1 cut(s)	2324
BaeGI	GKGCMC	2 cut(s)	2390, 4583
BalI	TGGCCA	1 cut(s)	1985
BamHI	GGATCC	2 cut(s)	1861, 4468
BanI	GGYRCC	1 cut(s)	4796
BanII	GRGCYC	1 cut(s)	3858
BauI	CACGAG	2 cut(s)	498, 4851
BbrPI	CACGTG	1 cut(s)	137
BbsI	GAAGAC	2 cut(s)	860, 4103
Bbv12I	GWGCWC	3 cut(s)	1018, 1653, 4583
BbvCI	CCTCAGC	3 cut(s)	1611, 1953, 4308
BbvI	GCAGC	9 cut(s)	1294, 1991, 2077, 2341, 3097, 3127, 3244, 4018, 4177
BceAI	ACGGC	2 cut(s)	987, 3248
BcgI	CGANNNNNNTGC	6 cut(s)	1489, 1523, 4570, 4604, 4952, 4986
BciT130I	CCWGG	3 cut(s)	1174, 3603, 3996
BciVI	GTATCC	6 cut(s)	379, 1179, 1276, 2868, 3836, 4002
BclI	TGATCA	5 cut(s)	300, 591, 2740, 3049, 3571
BcnI	CCSGG	1 cut(s)	2830
BcoDI	GTCTC	7 cut(s)	10, 281, 1085, 1330, 1800, 2400, 2646
BcuI	ACTAGT	1 cut(s)	1850
BfmI	CTRYAG	6 cut(s)	102, 2529, 2688, 3258, 3312, 4434
BfuAI	ACCTGC	2 cut(s)	86, 2974
BfuI	GTATCC	6 cut(s)	379, 1179, 1276, 2868, 3836, 4002
BglI	GCCNNNNNGGC	2 cut(s)	1982, 2087
Bme1390I	CCNGG	4 cut(s)	1174, 2830, 3603, 3996
Bme18I	GGWCC	8 cut(s)	6, 464, 873, 2378, 2807, 2963, 3466, 3892
BmeRI	GACNNNNNGTC	1 cut(s)	3216
BmgT120I	GGNCC	9 cut(s)	6, 373, 464, 873, 2378, 2807, 2963, 3466, 3892
BmiI	GGNNCC	8 cut(s)	7, 874, 875, 1863, 2964, 3946, 4470, 4798
BmrFI	CCNGG	4 cut(s)	1174, 2830, 3603, 3996
BoxI	GACNNNNGTC	1 cut(s)	3503
BpiI	GAAGAC	2 cut(s)	860, 4103
BpmI	CTGGAG	4 cut(s)	323, 1219, 1794, 3585
Bpu10I	CCTNAGC	4 cut(s)	1611, 1953, 3585, 4308
Bpu14I	TTCGAA	2 cut(s)	843, 2190
BpuEI	CTTGAG	6 cut(s)	2705, 2711, 3782, 4283, 4554, 4685
BpuMI	CCSGG	1 cut(s)	2830
BsaAI	YACGTR	2 cut(s)	137, 2789
BsaI	GGTCTC	1 cut(s)	2646
BsaJI	CCNNGG	3 cut(s)	449, 1172, 4185
BsaWI	WCCGGW	4 cut(s)	8, 174, 1058, 3941
BsaXI	ACNNNNNCTCC	4 cut(s)	1217, 1247, 2303, 2333
Bsc4I	CCNNNNNNNGG	9 cut(s)	382, 1138, 1535, 1853, 3684, 3901, 4142, 4381, 4460
Bse118I	RCCGGY	1 cut(s)	4355
Bse1I	ACTGG	7 cut(s)	523, 1236, 3019, 3469, 3639, 3700, 4126
Bse21I	CCTNAGG	1 cut(s)	2324
Bse3DI	GCAATG	4 cut(s)	684, 948, 1284, 2570
BseAI	TCCGGA	2 cut(s)	174, 1058
BseBI	CCWGG	3 cut(s)	1174, 3603, 3996
BseDI	CCNNGG	3 cut(s)	449, 1172, 4185
BseLI	CCNNNNNNNGG	9 cut(s)	382, 1138, 1535, 1853, 3684, 3901, 4142, 4381, 4460
BseMI	GCAATG	4 cut(s)	684, 948, 1284, 2570
BseNI	ACTGG	7 cut(s)	523, 1236, 3019, 3469, 3639, 3700, 4126
BseRI	GAGGAG	2 cut(s)	1595, 2327
BseSI	GKGCMC	2 cut(s)	2390, 4583
BseXI	GCAGC	9 cut(s)	1294, 1991, 2077, 2341, 3097, 3127, 3244, 4018, 4177
BseYI	CCCAGC	4 cut(s)	162, 375, 4091, 4632
BsgI	GTGCAG	2 cut(s)	1703, 2055
Bsh1236I	CGCG	3 cut(s)	457, 586, 809
BshFI	GGCC	9 cut(s)	374, 1026, 1518, 1739, 1985, 2833, 3677, 4608, 4779
BshNI	GGYRCC	1 cut(s)	4796
BsiHKAI	GWGCWC	3 cut(s)	1018, 1653, 4583
BsiSI	CCGG	6 cut(s)	9, 175, 1059, 2830, 3942, 4356
BslFI	GGGAC	7 cut(s)	19, 470, 886, 2976, 3416, 4159, 4614
BslI	CCNNNNNNNGG	9 cut(s)	382, 1138, 1535, 1853, 3684, 3901, 4142, 4381, 4460
BsmAI	GTCTC	7 cut(s)	10, 281, 1085, 1330, 1800, 2400, 2646
BsmFI	GGGAC	7 cut(s)	19, 470, 886, 2976, 3416, 4159, 4614
BsmI	GAATGC	1 cut(s)	2608
BsnI	GGCC	9 cut(s)	374, 1026, 1518, 1739, 1985, 2833, 3677, 4608, 4779
Bso31I	GGTCTC	1 cut(s)	2646
Bsp119I	TTCGAA	2 cut(s)	843, 2190
Bsp1286I	GDGCHC	5 cut(s)	1018, 1653, 2390, 3858, 4583
Bsp13I	TCCGGA	2 cut(s)	174, 1058
Bsp19I	CCATGG	1 cut(s)	449
BspANI	GGCC	9 cut(s)	374, 1026, 1518, 1739, 1985, 2833, 3677, 4608, 4779
BspEI	TCCGGA	2 cut(s)	174, 1058
BspFNI	CGCG	3 cut(s)	457, 586, 809
BspHI	TCATGA	1 cut(s)	2416
BspLI	GGNNCC	8 cut(s)	7, 874, 875, 1863, 2964, 3946, 4470, 4798
BspMAI	CTGCAG	2 cut(s)	2692, 3262
BspMI	ACCTGC	2 cut(s)	86, 2974
BspPI	GGATC	7 cut(s)	1856, 1869, 2233, 2414, 4421, 4463, 4476
BspQI	GCTCTTC	3 cut(s)	1470, 2500, 3645
BspT104I	TTCGAA	2 cut(s)	843, 2190
BspT107I	GGYRCC	1 cut(s)	4796
BspTNI	GGTCTC	1 cut(s)	2646
BsrDI	GCAATG	4 cut(s)	684, 948, 1284, 2570
BsrFI	RCCGGY	1 cut(s)	4355
BsrI	ACTGG	7 cut(s)	523, 1236, 3019, 3469, 3639, 3700, 4126
BssAI	RCCGGY	1 cut(s)	4355
BssECI	CCNNGG	3 cut(s)	449, 1172, 4185
BssSI	CACGAG	2 cut(s)	498, 4851
BssT1I	CCWWGG	2 cut(s)	449, 4185
Bst2BI	CACGAG	2 cut(s)	498, 4851
Bst2UI	CCWGG	3 cut(s)	1174, 3603, 3996
Bst6I	CTCTTC	7 cut(s)	249, 279, 1470, 1778, 2500, 3645, 3806
BstAPI	GCANNNNNTGC	3 cut(s)	2078, 2642, 4823
BstBAI	YACGTR	2 cut(s)	137, 2789
BstBI	TTCGAA	2 cut(s)	843, 2190
BstC8I	GCNNGC	9 cut(s)	345, 1014, 1050, 2647, 3108, 3156, 4011, 4353, 4357
BstDSI	CCRYGG	1 cut(s)	449
BstENI	CCTNNNNNAGG	3 cut(s)	1136, 1533, 3899
BstFNI	CGCG	3 cut(s)	457, 586, 809
BstHHI	GCGC	5 cut(s)	83, 588, 809, 1878, 3035
BstMAI	GTCTC	7 cut(s)	10, 281, 1085, 1330, 1800, 2400, 2646
BstNI	CCWGG	3 cut(s)	1174, 3603, 3996
BstNSI	RCATGY	4 cut(s)	2130, 3110, 4013, 4355
BstPAI	GACNNNNGTC	1 cut(s)	3503
BstSCI	CCNGG	4 cut(s)	1172, 2828, 3601, 3994
BstSFI	CTRYAG	6 cut(s)	102, 2529, 2688, 3258, 3312, 4434
BstSLI	GKGCMC	2 cut(s)	2390, 4583
BstUI	CGCG	3 cut(s)	457, 586, 809
BstV1I	GCAGC	9 cut(s)	1294, 1991, 2077, 2341, 3097, 3127, 3244, 4018, 4177
BstV2I	GAAGAC	2 cut(s)	860, 4103
BstX2I	RGATCY	3 cut(s)	1861, 4426, 4468
BstXI	CCANNNNNNTGG	2 cut(s)	2375, 3476
BstYI	RGATCY	3 cut(s)	1861, 4426, 4468
Bsu36I	CCTNAGG	1 cut(s)	2324
BsuI	GTATCC	6 cut(s)	379, 1179, 1276, 2868, 3836, 4002
BsuRI	GGCC	9 cut(s)	374, 1026, 1518, 1739, 1985, 2833, 3677, 4608, 4779
BtgI	CCRYGG	1 cut(s)	449
BtsI	GCAGTG	3 cut(s)	1752, 2076, 4542
BveI	ACCTGC	2 cut(s)	86, 2974
Cac8I	GCNNGC	9 cut(s)	345, 1014, 1050, 2647, 3108, 3156, 4011, 4353, 4357
CaiI	CAGNNNCTG	2 cut(s)	3099, 3881
CciI	TCATGA	1 cut(s)	2416
CfoI	GCGC	5 cut(s)	83, 588, 809, 1878, 3035
Cfr10I	RCCGGY	1 cut(s)	4355
Cfr13I	GGNCC	9 cut(s)	6, 373, 464, 873, 2378, 2807, 2963, 3466, 3892
CseI	GACGC	2 cut(s)	463, 3285
Csp6I	GTAC	7 cut(s)	551, 1111, 1142, 3076, 3935, 4028, 4289
CspCI	CAANNNNNGTGG	2 cut(s)	2875, 2910
CviQI	GTAC	7 cut(s)	551, 1111, 1142, 3076, 3935, 4028, 4289
DraI	TTTAAA	1 cut(s)	4641
DriI	GACNNNNNGTC	1 cut(s)	3216
EaeI	YGGCCR	2 cut(s)	1737, 1983
Eam1104I	CTCTTC	7 cut(s)	249, 279, 1470, 1778, 2500, 3645, 3806
Eam1105I	GACNNNNNGTC	1 cut(s)	3216
EarI	CTCTTC	7 cut(s)	249, 279, 1470, 1778, 2500, 3645, 3806
Eco130I	CCWWGG	2 cut(s)	449, 4185
Eco147I	AGGCCT	1 cut(s)	3677
Eco24I	GRGCYC	1 cut(s)	3858
Eco31I	GGTCTC	1 cut(s)	2646
Eco32I	GATATC	3 cut(s)	1503, 1621, 4503
Eco47I	GGWCC	8 cut(s)	6, 464, 873, 2378, 2807, 2963, 3466, 3892
Eco57I	CTGAAG	2 cut(s)	44, 1518
Eco72I	CACGTG	1 cut(s)	137
Eco81I	CCTNAGG	1 cut(s)	2324
EcoNI	CCTNNNNNAGG	3 cut(s)	1136, 1533, 3899
EcoO109I	RGGNCCY	2 cut(s)	873, 2963
EcoRII	CCWGG	3 cut(s)	1172, 3601, 3994
EcoRV	GATATC	3 cut(s)	1503, 1621, 4503
EcoT14I	CCWWGG	2 cut(s)	449, 4185
EcoT22I	ATGCAT	2 cut(s)	1364, 4828
EcoT38I	GRGCYC	1 cut(s)	3858
ErhI	CCWWGG	2 cut(s)	449, 4185
FalI	AAGNNNNNCTT	6 cut(s)	1637, 1669, 2463, 2495, 4245, 4277
FaqI	GGGAC	7 cut(s)	19, 470, 886, 2976, 3416, 4159, 4614
FauI	CCCGC	2 cut(s)	1041, 2654
FauNDI	CATATG	2 cut(s)	691, 3398
FbaI	TGATCA	5 cut(s)	300, 591, 2740, 3049, 3571
FblI	GTMKAC	1 cut(s)	3497
FriOI	GRGCYC	1 cut(s)	3858
GlaI	GCGC	5 cut(s)	82, 587, 808, 1877, 3034
GsaI	CCCAGC	4 cut(s)	166, 379, 4095, 4636
GsuI	CTGGAG	4 cut(s)	323, 1219, 1794, 3585
HaeIII	GGCC	9 cut(s)	374, 1026, 1518, 1739, 1985, 2833, 3677, 4608, 4779
HapII	CCGG	6 cut(s)	9, 175, 1059, 2830, 3942, 4356
HgaI	GACGC	2 cut(s)	463, 3285
HhaI	GCGC	5 cut(s)	83, 588, 809, 1878, 3035
Hin6I	GCGC	5 cut(s)	81, 586, 807, 1876, 3033
HinP1I	GCGC	5 cut(s)	81, 586, 807, 1876, 3033
HincII	GTYRAC	7 cut(s)	1423, 1723, 2595, 3221, 3498, 4119, 4199
HindII	GTYRAC	7 cut(s)	1423, 1723, 2595, 3221, 3498, 4119, 4199
HindIII	AAGCTT	2 cut(s)	2162, 4712
HpaII	CCGG	6 cut(s)	9, 175, 1059, 2830, 3942, 4356
HphI	GGTGA	9 cut(s)	56, 67, 146, 957, 1429, 1654, 3188, 4136, 4958
HpyCH4IV	ACGT	6 cut(s)	136, 306, 2788, 3325, 3557, 3646
HpySE526I	ACGT	6 cut(s)	136, 306, 2788, 3325, 3557, 3646
HspAI	GCGC	5 cut(s)	81, 586, 807, 1876, 3033
KflI	GGGWCCC	1 cut(s)	873
Kpn2I	TCCGGA	2 cut(s)	174, 1058
KroI	GCCGGC	1 cut(s)	4355
KroNI	GCCGGC	1 cut(s)	4357
Ksp22I	TGATCA	5 cut(s)	300, 591, 2740, 3049, 3571
LguI	GCTCTTC	3 cut(s)	1470, 2500, 3645
LmnI	GCTCC	5 cut(s)	342, 1035, 1481, 1648, 4088
Lsp1109I	GCAGC	9 cut(s)	1294, 1991, 2077, 2341, 3097, 3127, 3244, 4018, 4177
MaeII	ACGT	6 cut(s)	136, 306, 2788, 3325, 3557, 3646
MfeI	CAATTG	5 cut(s)	2664, 2937, 3531, 4410, 4473
MflI	RGATCY	3 cut(s)	1861, 4426, 4468
MhlI	GDGCHC	5 cut(s)	1018, 1653, 2390, 3858, 4583
MlsI	TGGCCA	1 cut(s)	1985
MluNI	TGGCCA	1 cut(s)	1985
MlyI	GAGTC	4 cut(s)	347, 2402, 2756, 4799
MmeI	TCCRAC	6 cut(s)	213, 518, 1013, 2122, 2852, 4582
Mox20I	TGGCCA	1 cut(s)	1985
Mph1103I	ATGCAT	2 cut(s)	1364, 4828
MroI	TCCGGA	2 cut(s)	174, 1058
MroNI	GCCGGC	1 cut(s)	4355
MroXI	GAANNNNTTC	1 cut(s)	1692
MscI	TGGCCA	1 cut(s)	1985
MslI	CAYNNNNRTG	4 cut(s)	1254, 2034, 3397, 3911
Msp20I	TGGCCA	1 cut(s)	1985
MspA1I	CMGCKG	2 cut(s)	22, 860
MspI	CCGG	6 cut(s)	9, 175, 1059, 2830, 3942, 4356
MspR9I	CCNGG	4 cut(s)	1174, 2830, 3603, 3996
MunI	CAATTG	5 cut(s)	2664, 2937, 3531, 4410, 4473
Mva1269I	GAATGC	1 cut(s)	2608
MvaI	CCWGG	3 cut(s)	1174, 3603, 3996
MvnI	CGCG	3 cut(s)	457, 586, 809
NaeI	GCCGGC	1 cut(s)	4357
NciI	CCSGG	1 cut(s)	2830
NcoI	CCATGG	1 cut(s)	449
NdeI	CATATG	2 cut(s)	691, 3398
NgoMIV	GCCGGC	1 cut(s)	4355
NlaIV	GGNNCC	8 cut(s)	7, 874, 875, 1863, 2964, 3946, 4470, 4798
NmeAIII	GCCGAG	1 cut(s)	4745
NmuCI	GTSAC	7 cut(s)	62, 73, 761, 1256, 2299, 3175, 3883
NsiI	ATGCAT	2 cut(s)	1364, 4828
NspI	RCATGY	4 cut(s)	2130, 3110, 4013, 4355
NspV	TTCGAA	2 cut(s)	843, 2190
OliI	CACNNNNGTG	1 cut(s)	3911
PaeI	GCATGC	3 cut(s)	3110, 4013, 4355
PagI	TCATGA	1 cut(s)	2416
PaqCI	CACCTGC	1 cut(s)	86
PceI	AGGCCT	1 cut(s)	3677
PciI	ACATGT	1 cut(s)	2126
PciSI	GCTCTTC	3 cut(s)	1470, 2500, 3645
PctI	GAATGC	1 cut(s)	2608
PdiI	GCCGGC	1 cut(s)	4357
PdmI	GAANNNNTTC	1 cut(s)	1692
PflFI	GACNNNGTC	1 cut(s)	292
PfoI	TCCNGGA	2 cut(s)	3601, 3994
PleI	GAGTC	4 cut(s)	347, 2401, 2755, 4799
PmaCI	CACGTG	1 cut(s)	137
PmlI	CACGTG	1 cut(s)	137
PpsI	GAGTC	4 cut(s)	347, 2401, 2755, 4799
Ppu21I	YACGTR	2 cut(s)	137, 2789
PpuMI	RGGWCCY	2 cut(s)	873, 2963
PscI	ACATGT	1 cut(s)	2126
PshAI	GACNNNNGTC	1 cut(s)	3503
PshBI	ATTAAT	1 cut(s)	1892
PsiI	TTATAA	3 cut(s)	362, 1832, 2469
Psp5II	RGGWCCY	2 cut(s)	873, 2963
Psp6I	CCWGG	3 cut(s)	1172, 3601, 3994
PspCI	CACGTG	1 cut(s)	137
PspFI	CCCAGC	4 cut(s)	162, 375, 4091, 4632
PspGI	CCWGG	3 cut(s)	1172, 3601, 3994
PspN4I	GGNNCC	8 cut(s)	7, 874, 875, 1863, 2964, 3946, 4470, 4798
PspPI	GGNCC	9 cut(s)	6, 373, 464, 873, 2378, 2807, 2963, 3466, 3892
PspPPI	RGGWCCY	2 cut(s)	873, 2963
PstI	CTGCAG	2 cut(s)	2692, 3262
PstNI	CAGNNNCTG	2 cut(s)	3099, 3881
PsuI	RGATCY	3 cut(s)	1861, 4426, 4468
PsyI	GACNNNGTC	1 cut(s)	292
PvuII	CAGCTG	1 cut(s)	22
RsaI	GTAC	7 cut(s)	552, 1112, 1143, 3077, 3936, 4029, 4290
RsaNI	GTAC	7 cut(s)	551, 1111, 1142, 3076, 3935, 4028, 4289
RseI	CAYNNNNRTG	4 cut(s)	1254, 2034, 3397, 3911
SalI	GTCGAC	1 cut(s)	3496
SapI	GCTCTTC	3 cut(s)	1470, 2500, 3645
Sau96I	GGNCC	9 cut(s)	6, 373, 464, 873, 2378, 2807, 2963, 3466, 3892
SchI	GAGTC	4 cut(s)	347, 2402, 2756, 4799
ScrFI	CCNGG	4 cut(s)	1174, 2830, 3603, 3996
SduI	GDGCHC	5 cut(s)	1018, 1653, 2390, 3858, 4583
SfcI	CTRYAG	6 cut(s)	102, 2529, 2688, 3258, 3312, 4434
SfuI	TTCGAA	2 cut(s)	843, 2190
SinI	GGWCC	8 cut(s)	6, 464, 873, 2378, 2807, 2963, 3466, 3892
SmiMI	CAYNNNNRTG	4 cut(s)	1254, 2034, 3397, 3911
SmlI	CTYRAG	6 cut(s)	2720, 2726, 3797, 4262, 4569, 4664
SmoI	CTYRAG	6 cut(s)	2720, 2726, 3797, 4262, 4569, 4664
SpeI	ACTAGT	1 cut(s)	1850
SphI	GCATGC	3 cut(s)	3110, 4013, 4355
SseBI	AGGCCT	1 cut(s)	3677
SspI	AATATT	4 cut(s)	1216, 1564, 1662, 4241
StuI	AGGCCT	1 cut(s)	3677
StyD4I	CCNGG	4 cut(s)	1172, 2828, 3601, 3994
StyI	CCWWGG	2 cut(s)	449, 4185
TaiI	ACGT	6 cut(s)	139, 309, 2791, 3328, 3560, 3649
TatI	WGTACW	2 cut(s)	550, 1110
TauI	GCSGC	2 cut(s)	374, 4909
TseFI	GTSAC	7 cut(s)	62, 73, 761, 1256, 2299, 3175, 3883
TseI	GCWGC	9 cut(s)	1307, 2004, 2090, 2354, 3110, 3140, 3257, 4006, 4190
Tsp45I	GTSAC	7 cut(s)	62, 73, 761, 1256, 2299, 3175, 3883
TspGWI	ACGGA	6 cut(s)	456, 467, 1603, 3537, 3636, 4154
Tth111I	GACNNNGTC	1 cut(s)	292
VneI	GTGCAC	1 cut(s)	4579
VpaK11BI	GGWCC	8 cut(s)	6, 464, 873, 2378, 2807, 2963, 3466, 3892
VspI	ATTAAT	1 cut(s)	1892
XagI	CCTNNNNNAGG	3 cut(s)	1136, 1533, 3899
XapI	RAATTY	5 cut(s)	57, 320, 1066, 2903, 2987
XceI	RCATGY	4 cut(s)	2130, 3110, 4013, 4355
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2083, 3026
XmiI	GTMKAC	1 cut(s)	3497
XmnI	GAANNNNTTC	1 cut(s)	1692
Zsp2I	ATGCAT	2 cut(s)	1364, 4828

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.