FvH4_6g24120

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008651
Physical Location & Seq
Reverse (-)
18166191 .. 18167982
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24120.t1

Sequence Viewer

Length: 711 bp
ATGGAGGGTGTGTGTGATCAAAAGGTTCTGTTGGAATACGTCACGAGGAAGTCGAGCCCGCCGCCTTTCTTGTTCAAGACGTACATGCTGGTGGAGGATCCGGCAACCGACGCCGTGATATCGTGGAATGAAGACGGGTCGGCGTTCGTCGTTTGGCAGCCAGCCGAGTTTGCCCGAGATCTCCTCCCAACTCTCTTCAAGCACAGTAACTTCTCTAGCTTTGTCAGGCAGCTCAATACTTACGGATTTCGCAAAGTTGCAACCAGCCGGTGGGAGTTTTGCAACGACAAGTTTCGAAAGGGTGAAAAGGATCATCTGTGTGAAATCCGTCGTAGAAAAGCATGGTCGAACAAGCAACAGCCAAATCAGCAAGGCCAAGCAGCAGGAGGAGAAGATGACCAGTCTGATGAAAGGTCTTCGTCGACTTCTTCTTCGTCTGAGTACAGCTCTCTTATTGATGAAAACAGACGGCTGAAGATGGAAAATGGGGTTTTGAGCTGCGAGCTTACGAGCATGAAAAACAAGTGCAAGGAGCTTCTCGATTTGGTGGCCAAGAATGGCCGGGATGCGGATCAAGAGGAAGACGACGACCAAAGGCCAAAGCTGTTTGGTGTGAGATTGGAGGTTGAGGGAGAGAGGGAAAGAAAGAGAAAGAGGGCAGAGATAATTAGTGAAAGCGCAAGCATTTTACTATCTCAACCATGCAAATAA

Protein Analysis

237

Amino Acids

27.1

Weight (kDa)

5.91

Isoelectric Point (pI)

58.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 23 - 112 1.3e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 270
AccI GTMKAC 1 cut(s) 422
AciI CCGC 3 cut(s) 59, 62, 569
AclWI GGATC 4 cut(s) 92, 105, 318, 579
AcoI YGGCCR 2 cut(s) 549, 559
AcuI CTGAAG 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 2 cut(s) 83, 443
AfiI CCNNNNNNNGG 2 cut(s) 270, 568
AgsI TTSAA 2 cut(s) 76, 199
AluBI AGCT 7 cut(s) 219, 232, 447, 498, 505, 535, 604
AluI AGCT 7 cut(s) 219, 232, 447, 498, 505, 535, 604
AlwI GGATC 4 cut(s) 92, 105, 318, 579
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 4 cut(s) 373, 549, 559, 596
ApeKI GCWGC 4 cut(s) 157, 229, 380, 498
ArsI GACNNNNNNTTYG 2 cut(s) 415, 447
AspLEI GCGC 1 cut(s) 680
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 1 cut(s) 314
AsuII TTCGAA 1 cut(s) 295
AvaI CYCGRG 1 cut(s) 174
BalI TGGCCA 1 cut(s) 551
BamHI GGATCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 59
BauI CACGAG 1 cut(s) 43
BbsI GAAGAC 3 cut(s) 138, 408, 588
BbvI GCAGC 4 cut(s) 169, 241, 392, 485
BccI CCATC 1 cut(s) 472
BceAI ACGGC 2 cut(s) 98, 485
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 1 cut(s) 563
BfaI CTAG 1 cut(s) 216
BglII AGATCT 1 cut(s) 178
BisI GCNGC 5 cut(s) 62, 158, 230, 381, 499
BlsI GCNGC 5 cut(s) 63, 159, 231, 382, 500
Bme1390I CCNGG 1 cut(s) 563
BmeT110I CYCGRG 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 1 cut(s) 556
BpiI GAAGAC 3 cut(s) 138, 408, 588
BplI GAGNNNNNCTC 4 cut(s) 168, 200, 431, 463
Bpu14I TTCGAA 1 cut(s) 295
BpuMI CCSGG 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 1 cut(s) 111
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 568
Bse118I RCCGGY 1 cut(s) 267
Bse1I ACTGG 1 cut(s) 400
Bse8I GATNNNNATC 1 cut(s) 570
BseGI GGATG 1 cut(s) 571
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 2 cut(s) 270, 568
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 2 cut(s) 173, 402
BseXI GCAGC 4 cut(s) 169, 241, 392, 485
BshFI GGCC 4 cut(s) 375, 551, 561, 598
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 3 cut(s) 101, 268, 562
BslI CCNNNNNNNGG 2 cut(s) 270, 568
BsnI GGCC 4 cut(s) 375, 551, 561, 598
BsoBI CYCGRG 1 cut(s) 174
Bsp119I TTCGAA 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 5 cut(s) 16, 97, 178, 310, 571
BspACI CCGC 3 cut(s) 59, 62, 569
BspANI GGCC 4 cut(s) 375, 551, 561, 598
BspCNI CTCAG 1 cut(s) 430
BspLI GGNNCC 1 cut(s) 99
BspPI GGATC 4 cut(s) 92, 105, 318, 579
BspT104I TTCGAA 1 cut(s) 295
BsrFI RCCGGY 1 cut(s) 267
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 1 cut(s) 267
BssMI GATC 5 cut(s) 16, 97, 178, 310, 571
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 43
Bst2BI CACGAG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 206
Bst6I CTCTTC 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 111
BstBI TTCGAA 1 cut(s) 295
BstC8I GCNNGC 4 cut(s) 59, 162, 503, 682
BstDEI CTNAG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 571
BstHHI GCGC 1 cut(s) 680
BstKTI GATC 5 cut(s) 19, 100, 181, 313, 574
BstMBI GATC 5 cut(s) 16, 97, 178, 310, 571
BstMWI GCNNNNNNNGC 3 cut(s) 110, 170, 367
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 561
BstV1I GCAGC 4 cut(s) 169, 241, 392, 485
BstV2I GAAGAC 3 cut(s) 138, 408, 588
BstX2I RGATCY 2 cut(s) 97, 178
BstYI RGATCY 2 cut(s) 97, 178
BsuRI GGCC 4 cut(s) 375, 551, 561, 598
BtsCI GGATG 1 cut(s) 571
Cac8I GCNNGC 4 cut(s) 59, 162, 503, 682
CfoI GCGC 1 cut(s) 680
Cfr10I RCCGGY 1 cut(s) 267
CseI GACGC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 82, 442
CviAII CATG 4 cut(s) 85, 342, 514, 702
CviQI GTAC 2 cut(s) 82, 442
DdeI CTNAG 1 cut(s) 438
DpnI GATC 5 cut(s) 18, 99, 180, 312, 573
DpnII GATC 5 cut(s) 16, 97, 178, 310, 571
EaeI YGGCCR 2 cut(s) 549, 559
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 59
Eco32I GATATC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 174
EcoRV GATATC 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 4 cut(s) 88, 345, 517, 705
FaiI YATR 4 cut(s) 86, 343, 515, 703
FatI CATG 4 cut(s) 84, 341, 513, 701
FauI CCCGC 1 cut(s) 66
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 1 cut(s) 422
Fnu4HI GCNGC 5 cut(s) 62, 158, 230, 381, 499
FokI GGATG 1 cut(s) 578
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 5 cut(s) 62, 158, 230, 381, 499
FspBI CTAG 1 cut(s) 216
GlaI GCGC 1 cut(s) 679
GluI GCNGC 5 cut(s) 62, 158, 230, 381, 499
HaeIII GGCC 4 cut(s) 375, 551, 561, 598
HapII CCGG 3 cut(s) 101, 268, 562
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 680
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 4 cut(s) 88, 345, 517, 705
Hin6I GCGC 1 cut(s) 678
HinP1I GCGC 1 cut(s) 678
HincII GTYRAC 1 cut(s) 423
HindII GTYRAC 1 cut(s) 423
HpaII CCGG 3 cut(s) 101, 268, 562
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 423
Hpy188I TCNGA 2 cut(s) 406, 439
Hpy188III TCNNGA 4 cut(s) 43, 76, 539, 575
Hpy8I GTNNAC 1 cut(s) 423
Hpy99I CGWCG 5 cut(s) 113, 152, 333, 424, 590
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 2 cut(s) 39, 80
HpyCH4V TGCA 4 cut(s) 260, 282, 528, 705
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 170, 367
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 2 cut(s) 39, 80
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 4 cut(s) 88, 345, 517, 705
HspAI GCGC 1 cut(s) 678
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 97, 178, 310, 571
LmnI GCTCC 1 cut(s) 532
LpnPI CCDG 9 cut(s) 74, 114, 174, 211, 277, 281, 369, 413, 575
Lsp1109I GCAGC 4 cut(s) 169, 241, 392, 485
LweI GCATC 1 cut(s) 556
MaeI CTAG 1 cut(s) 216
MaeII ACGT 2 cut(s) 39, 80
MaeIII GTNAC 2 cut(s) 40, 206
MalI GATC 5 cut(s) 18, 99, 180, 312, 573
MboI GATC 5 cut(s) 16, 97, 178, 310, 571
MboII GAAGA 8 cut(s) 143, 187, 404, 408, 420, 423, 487, 593
MflI RGATCY 2 cut(s) 97, 178
MhlI GDGCHC 1 cut(s) 59
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 1 cut(s) 666
MluNI TGGCCA 1 cut(s) 551
MmeI TCCRAC 1 cut(s) 12
MnlI CCTC 9 cut(s) 39, 88, 194, 380, 571, 616, 622, 630, 648
Mox20I TGGCCA 1 cut(s) 551
MscI TGGCCA 1 cut(s) 551
MslI CAYNNNNRTG 2 cut(s) 89, 318
Msp20I TGGCCA 1 cut(s) 551
MspI CCGG 3 cut(s) 101, 268, 562
MspR9I CCNGG 1 cut(s) 563
MwoI GCNNNNNNNGC 3 cut(s) 110, 170, 367
NciI CCSGG 1 cut(s) 563
NdeII GATC 5 cut(s) 16, 97, 178, 310, 571
NlaIII CATG 4 cut(s) 88, 345, 517, 705
NlaIV GGNNCC 1 cut(s) 99
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 40
NspI RCATGY 1 cut(s) 88
NspV TTCGAA 1 cut(s) 295
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 5 cut(s) 63, 159, 231, 382, 500
PspN4I GGNNCC 1 cut(s) 99
PsuI RGATCY 2 cut(s) 97, 178
RsaI GTAC 2 cut(s) 83, 443
RsaNI GTAC 2 cut(s) 82, 442
RseI CAYNNNNRTG 2 cut(s) 89, 318
SalI GTCGAC 1 cut(s) 421
SatI GCNGC 5 cut(s) 62, 158, 230, 381, 499
Sau3AI GATC 5 cut(s) 16, 97, 178, 310, 571
ScrFI CCNGG 1 cut(s) 563
SduI GDGCHC 1 cut(s) 59
SfaNI GCATC 1 cut(s) 556
SfuI TTCGAA 1 cut(s) 295
SmiMI CAYNNNNRTG 2 cut(s) 89, 318
Sse9I AATT 1 cut(s) 666
SsiI CCGC 3 cut(s) 59, 62, 569
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 561
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 2 cut(s) 42, 83
TaqI TCGA 5 cut(s) 53, 295, 347, 422, 540
TasI AATT 1 cut(s) 666
TatI WGTACW 1 cut(s) 441
TauI GCSGC 1 cut(s) 64
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 4 cut(s) 157, 229, 380, 498
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 4 cut(s) 144, 423, 474, 530
TspGWI ACGGA 2 cut(s) 258, 317
Van91I CCANNNNNTGG 1 cut(s) 270
XceI RCATGY 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 422
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.