MD14G1075200.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008651
Physical Location & Seq
Forward (+)
8530508 .. 8532527
2020 bp
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UTR
Exon/CDS
Intron
MD14G1075200.v1.1.491

Sequence Viewer

Length: 249 bp
ATGGAGGGTGTGTGTGATGATCAGAAGCGTTTGTTGGAATATGTGAGGAAGTCAAGCCCACCACCTTTCTTGTTGAAGACCTACATGCTGGTGGAGGATCCGGCGACGGACGATGTGATATCCTGGAACGACGATGGGTCGGCGTTCGTGGTGTGGCAGACGGCGGAATTTGCCCGGGATCTCCTCCCAACACTCTTCAAGCATAGTAACTTCTCTAGCTTTGTCAGGCAGCTAAATACTTATGGGTGA

Protein Analysis

83

Amino Acids

9.45

Weight (kDa)

4.68

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 23 - 82 1.3e-21 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 164
AclWI GGATC 3 cut(s) 92, 105, 186
AcsI RAATTY 1 cut(s) 167
AgsI TTSAA 2 cut(s) 76, 199
AhdI GACNNNNNGTC 1 cut(s) 136
AjnI CCWGG 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 2 cut(s) 219, 232
AluI AGCT 2 cut(s) 219, 232
AlwI GGATC 3 cut(s) 92, 105, 186
Ama87I CYCGRG 1 cut(s) 174
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 167
AsuC2I CCSGG 2 cut(s) 175, 176
AvaI CYCGRG 1 cut(s) 174
BamHI GGATCC 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 83
BbvI GCAGC 1 cut(s) 241
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 177
BciT130I CCWGG 1 cut(s) 124
BclI TGATCA 1 cut(s) 19
BcnI CCSGG 2 cut(s) 175, 176
BfaI CTAG 1 cut(s) 216
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
Bme1390I CCNGG 3 cut(s) 124, 175, 176
BmeRI GACNNNNNGTC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 3 cut(s) 124, 175, 176
BpiI GAAGAC 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 175, 176
BsaJI CCNNGG 1 cut(s) 174
BsaXI ACNNNNNCTCC 1 cut(s) 26
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 174
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 241
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 2 cut(s) 101, 175
BsoBI CYCGRG 1 cut(s) 174
Bsp143I GATC 3 cut(s) 19, 97, 178
BspACI CCGC 1 cut(s) 164
BspLI GGNNCC 1 cut(s) 99
BspPI GGATC 3 cut(s) 92, 105, 186
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 3 cut(s) 19, 97, 178
Bst2UI CCWGG 1 cut(s) 124
Bst6I CTCTTC 1 cut(s) 200
BstKTI GATC 3 cut(s) 22, 100, 181
BstMBI GATC 3 cut(s) 19, 97, 178
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 3 cut(s) 122, 173, 174
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 2 cut(s) 97, 178
BstYI RGATCY 2 cut(s) 97, 178
Cfr9I CCCGGG 1 cut(s) 174
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
CviAII CATG 1 cut(s) 85
CviJI RGCY 3 cut(s) 57, 219, 232
CviKI_1 RGCY 3 cut(s) 57, 219, 232
DpnI GATC 3 cut(s) 21, 99, 180
DpnII GATC 3 cut(s) 19, 97, 178
DriI GACNNNNNGTC 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 200
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 200
EciI GGCGGA 1 cut(s) 179
Eco32I GATATC 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 174
EcoRII CCWGG 1 cut(s) 122
EcoRV GATATC 1 cut(s) 120
FaeI CATG 1 cut(s) 88
FaiI YATR 4 cut(s) 42, 86, 204, 243
FatI CATG 1 cut(s) 84
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 230
FspBI CTAG 1 cut(s) 216
GluI GCNGC 1 cut(s) 230
HapII CCGG 2 cut(s) 101, 175
Hin1II CATG 1 cut(s) 88
HpaII CCGG 2 cut(s) 101, 175
Hpy188I TCNGA 1 cut(s) 24
Hpy99I CGWCG 2 cut(s) 109, 134
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
Hsp92II CATG 1 cut(s) 88
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 3 cut(s) 19, 97, 178
LpnPI CCDG 6 cut(s) 74, 109, 114, 136, 188, 211
Lsp1109I GCAGC 1 cut(s) 241
MaeI CTAG 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 206
MalI GATC 3 cut(s) 21, 99, 180
MboI GATC 3 cut(s) 19, 97, 178
MboII GAAGA 2 cut(s) 88, 187
MflI RGATCY 2 cut(s) 97, 178
MluCI AATT 1 cut(s) 167
MmeI TCCRAC 1 cut(s) 15
MnlI CCTC 3 cut(s) 39, 88, 194
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 2 cut(s) 101, 175
MspR9I CCNGG 3 cut(s) 124, 175, 176
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 1 cut(s) 170
NciI CCSGG 2 cut(s) 175, 176
NdeII GATC 3 cut(s) 19, 97, 178
NlaIII CATG 1 cut(s) 88
NlaIV GGNNCC 1 cut(s) 99
NspI RCATGY 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 99
PsuI RGATCY 2 cut(s) 97, 178
RseI CAYNNNNRTG 1 cut(s) 89
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 3 cut(s) 19, 97, 178
ScrFI CCNGG 3 cut(s) 124, 175, 176
SetI ASST 4 cut(s) 67, 83, 221, 234
SmaI CCCGGG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 1 cut(s) 167
SsiI CCGC 1 cut(s) 164
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 3 cut(s) 122, 173, 174
TasI AATT 1 cut(s) 167
TseI GCWGC 1 cut(s) 229
TspGWI ACGGA 1 cut(s) 122
TspMI CCCGGG 1 cut(s) 174
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 88
XmaI CCCGGG 1 cut(s) 174
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.