pycom14g06400

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008651
Physical Location & Seq
Forward (+)
6316158 .. 6319676
3519 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06400.1

Sequence Viewer

Length: 732 bp
ATGGAGGGTGTGTGTGATGATCAGAAGCGTTTGTTGGAATATGTGAGGAAGTCAAGCCCACCACCTTTCTTGTTGAAGACCTACATGCTGGTGGAGGATCCGGCGACGGACGATGTGATATCCTGGAACGACGATGGGTCGGCGTTCGTGGTGTGGCAGACGGCGGAATTTGCCCGGGATCTCCTCCCAACACTCTTCAAGCATAGTAACTTCTCTAGCTTTGTCAGGCAGCTAAATACTTATGGATTTCGCAAAGTTGCCACAAACAGGTGGGAGTTCTGCAACGACAAGTTCCGAAAGGGCGAAAAGGATCAGCGTTGTGACATCCGTAGAAGAAAAGCATGGGCCACCAAGCAACAGCCGATCAACAATGCAGTAACCCAACAAGCTGGAGCAGCTGTACTACTACCAAATGAGTTTGATGAAGACCAAAGGTCCTCCTCAACTTCATCATCATCCGAGTTTAGCTCTCTCGTCGACGAAAACAAAAGGCTGAAGCAGGAGAACGGGGTTTTGAGCTCGGAGCTGACGAGCATGAAACGGAAGTGCAAGGAGCTTCTTGACTTGGTGGCAAAGTATGGAGACTCGGCTGAGAAAGAGGAGGAAGATAATGAAAGAGTGCTAAAGTTGTTTGGAGTGAGATTGGAGGTTACTGGAGAGAGGGAGAGGAAGAGAAAGAGAGCTGAAATTAGTGCAACCGCAAACGTTTTACTATCTCAAGCATGCAAATAA

Protein Analysis

244

Amino Acids

27.89

Weight (kDa)

8.2

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 23 - 112 5.9e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 477
AciI CCGC 2 cut(s) 164, 699
AclI AACGTT 1 cut(s) 705
AclWI GGATC 4 cut(s) 92, 105, 186, 318
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 2 cut(s) 76, 199
AhdI GACNNNNNGTC 2 cut(s) 136, 433
AjnI CCWGG 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 9 cut(s) 219, 232, 389, 398, 468, 519, 526, 556, 683
AluI AGCT 9 cut(s) 219, 232, 389, 398, 468, 519, 526, 556, 683
Alw21I GWGCWC 1 cut(s) 521
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 4 cut(s) 92, 105, 186, 318
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 2 cut(s) 229, 395
ApoI RAATTY 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 345, 435
AsuC2I CCSGG 2 cut(s) 175, 176
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 1 cut(s) 435
BamHI GGATCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 83, 432
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 2 cut(s) 241, 407
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 177
BciT130I CCWGG 1 cut(s) 124
BclI TGATCA 1 cut(s) 19
BcnI CCSGG 2 cut(s) 175, 176
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 1 cut(s) 216
BisI GCNGC 2 cut(s) 230, 396
BlsI GCNGC 2 cut(s) 231, 397
Bme1390I CCNGG 3 cut(s) 124, 175, 176
Bme18I GGWCC 1 cut(s) 435
BmeRI GACNNNNNGTC 2 cut(s) 136, 433
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 2 cut(s) 345, 435
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 3 cut(s) 124, 175, 176
BpiI GAAGAC 2 cut(s) 83, 432
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 2 cut(s) 411, 675
BpuEI CTTGAG 1 cut(s) 702
BpuMI CCSGG 2 cut(s) 175, 176
BsaJI CCNNGG 1 cut(s) 174
BsaXI ACNNNNNCTCC 3 cut(s) 26, 384, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse1I ACTGG 1 cut(s) 658
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 2 cut(s) 324, 455
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 1 cut(s) 658
BseRI GAGGAG 3 cut(s) 173, 430, 614
BseXI GCAGC 2 cut(s) 241, 407
BshFI GGCC 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 2 cut(s) 101, 175
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 576
BsnI GGCC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 521
Bsp143I GATC 5 cut(s) 19, 97, 178, 310, 363
BspACI CCGC 2 cut(s) 164, 699
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 1 cut(s) 583
BspLI GGNNCC 1 cut(s) 99
BspPI GGATC 4 cut(s) 92, 105, 186, 318
BsrI ACTGG 1 cut(s) 658
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 5 cut(s) 19, 97, 178, 310, 363
Bst2UI CCWGG 1 cut(s) 124
Bst6I CTCTTC 2 cut(s) 200, 665
BstC8I GCNNGC 1 cut(s) 724
BstDEI CTNAG 1 cut(s) 591
BstF5I GGATG 2 cut(s) 324, 455
BstKTI GATC 5 cut(s) 22, 100, 181, 313, 366
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 5 cut(s) 19, 97, 178, 310, 363
BstMWI GCNNNNNNNGC 2 cut(s) 170, 395
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 2 cut(s) 88, 726
BstSCI CCNGG 3 cut(s) 122, 173, 174
BstV1I GCAGC 2 cut(s) 241, 407
BstV2I GAAGAC 2 cut(s) 83, 432
BstX2I RGATCY 2 cut(s) 97, 178
BstXI CCANNNNNNTGG 1 cut(s) 389
BstYI RGATCY 2 cut(s) 97, 178
BsuRI GGCC 1 cut(s) 347
BtsCI GGATG 2 cut(s) 324, 455
Cac8I GCNNGC 1 cut(s) 724
Cfr13I GGNCC 2 cut(s) 345, 435
Cfr9I CCCGGG 1 cut(s) 174
Csp6I GTAC 1 cut(s) 401
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
CviAII CATG 4 cut(s) 85, 342, 535, 723
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 1 cut(s) 591
DpnI GATC 5 cut(s) 21, 99, 180, 312, 365
DpnII GATC 5 cut(s) 19, 97, 178, 310, 363
DriI GACNNNNNGTC 2 cut(s) 136, 433
Eam1104I CTCTTC 2 cut(s) 200, 665
Eam1105I GACNNNNNGTC 2 cut(s) 136, 433
EarI CTCTTC 2 cut(s) 200, 665
EciI GGCGGA 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 521
Eco32I GATATC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 122
EcoRV GATATC 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 521
FaeI CATG 4 cut(s) 88, 345, 538, 726
FaiI YATR 8 cut(s) 42, 86, 204, 243, 343, 536, 579, 724
FatI CATG 4 cut(s) 84, 341, 534, 722
FbaI TGATCA 1 cut(s) 19
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 2 cut(s) 230, 396
FokI GGATG 2 cut(s) 311, 442
FriOI GRGCYC 1 cut(s) 521
Fsp4HI GCNGC 2 cut(s) 230, 396
FspBI CTAG 1 cut(s) 216
GluI GCNGC 2 cut(s) 230, 396
GsuI CTGGAG 2 cut(s) 411, 675
HaeIII GGCC 1 cut(s) 347
HapII CCGG 2 cut(s) 101, 175
Hin1II CATG 4 cut(s) 88, 345, 538, 726
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 1 cut(s) 584
HpaII CCGG 2 cut(s) 101, 175
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 4 cut(s) 24, 296, 460, 523
Hpy188III TCNNGA 1 cut(s) 560
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 4 cut(s) 109, 134, 479, 482
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 5 cut(s) 282, 374, 549, 695, 726
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 395
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 1 cut(s) 705
Hsp92II CATG 4 cut(s) 88, 345, 538, 726
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 5 cut(s) 19, 97, 178, 310, 363
LmnI GCTCC 3 cut(s) 392, 523, 553
Lsp1109I GCAGC 2 cut(s) 241, 407
MaeI CTAG 1 cut(s) 216
MaeII ACGT 1 cut(s) 705
MaeIII GTNAC 4 cut(s) 206, 320, 376, 649
MalI GATC 5 cut(s) 21, 99, 180, 312, 365
MboI GATC 5 cut(s) 19, 97, 178, 310, 363
MboII GAAGA 6 cut(s) 88, 187, 345, 437, 617, 682
MflI RGATCY 2 cut(s) 97, 178
MhlI GDGCHC 1 cut(s) 521
MluCI AATT 2 cut(s) 167, 687
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 2 cut(s) 101, 175
MspR9I CCNGG 3 cut(s) 124, 175, 176
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 2 cut(s) 170, 395
NciI CCSGG 2 cut(s) 175, 176
NdeII GATC 5 cut(s) 19, 97, 178, 310, 363
NlaIII CATG 4 cut(s) 88, 345, 538, 726
NlaIV GGNNCC 1 cut(s) 99
NmeAIII GCCGAG 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 320
NspI RCATGY 2 cut(s) 88, 726
PaeI GCATGC 1 cut(s) 726
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 2 cut(s) 231, 397
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
PpuMI RGGWCCY 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 521
Psp1406I AACGTT 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 2 cut(s) 345, 435
PspPPI RGGWCCY 1 cut(s) 435
PsuI RGATCY 2 cut(s) 97, 178
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 1 cut(s) 521
SalI GTCGAC 1 cut(s) 476
SatI GCNGC 2 cut(s) 230, 396
Sau3AI GATC 5 cut(s) 19, 97, 178, 310, 363
Sau96I GGNCC 2 cut(s) 345, 435
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 3 cut(s) 124, 175, 176
SduI GDGCHC 1 cut(s) 521
SgrDI CGTCGACG 1 cut(s) 476
SinI GGWCC 1 cut(s) 435
SmaI CCCGGG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
SphI GCATGC 1 cut(s) 726
Sse9I AATT 2 cut(s) 167, 687
SsiI CCGC 2 cut(s) 164, 699
SspMI CTAG 1 cut(s) 216
SstI GAGCTC 1 cut(s) 521
StyD4I CCNGG 3 cut(s) 122, 173, 174
TaiI ACGT 1 cut(s) 708
TaqI TCGA 1 cut(s) 477
TasI AATT 2 cut(s) 167, 687
TatI WGTACW 1 cut(s) 400
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 2 cut(s) 229, 395
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 4 cut(s) 438, 438, 551, 627
TspGWI ACGGA 3 cut(s) 122, 317, 556
TspMI CCCGGG 1 cut(s) 174
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 1 cut(s) 167
XceI RCATGY 2 cut(s) 88, 726
XmaI CCCGGG 1 cut(s) 174
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.