MD12G1079300.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008651
Physical Location & Seq
Forward (+)
9679614 .. 9683728
4115 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1079300.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGAGGGTGTGTGTGATGAGCAGAAGGGTTTGTTGGAATATGTTAGGAAGTCAAGCCCGCCACCTTTCCTGTTGAAGACCTACATGCTGGTGGAGGATCCAGCAACGGACGACGTGATATCCTGGAACGACGATGGGTCGGCGTTCGTGGTGTGGCAGACGGCGGAATTTGCCCGGGATCTCCTCCCAACACTCTTCAAGCACAGTAACTTCTCTAGCTTTATCAGGCAGCTAAATACTTATGGATTTCGCAAAGTTGCCACAAACAGGTGGGAGTTCTGCAACGACAAGTTCCGAAAGGGCGAAAAGGATCAGCTATGTGACATCCGTAGAAGAAAAGCATGGGCCACCAAGCCACAGCCGATCAACAATGCAGTAACCGAGAAAGCTGGAGCAGCTGCACTACTACCAAATGAGTTTGATGAAGACCAAAGGTCCTCCTCAACTTCATCATCATCTGAGTTCTGCTCTCTCGTTGACGAAAACAAACGGCTGAAGCAGGAGAACGGCGTTTTGAGCTCAGAGCTTATGAGCATGAAACAGAAGTGCAACGAGCTTCTCCATTTGGTGGCAAGGTATGGAGACTCAGCTGAGAAAGAGGAGGAAGATAATGAAAGAGTTCCAAAGTTGTTTGGAGTGAGACTGGAGGTTGCGGGAGAGAGGGAGAGGAAGAGAAAGAGAGCTGAAATTAGTAAAAACGCAAGCGTTTTACTATCTCAAGCATGCAAATAA

Protein Analysis

244

Amino Acids

27.77

Weight (kDa)

6.77

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 23 - 112 7.2e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 568
AciI CCGC 3 cut(s) 59, 164, 653
AclWI GGATC 4 cut(s) 92, 105, 186, 318
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 515
AfiI CCNNNNNNNGG 2 cut(s) 267, 568
AgsI TTSAA 2 cut(s) 76, 199
AhdI GACNNNNNGTC 2 cut(s) 136, 433
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
Alw21I GWGCWC 1 cut(s) 521
Alw26I GTCTC 2 cut(s) 576, 634
AlwI GGATC 4 cut(s) 92, 105, 186, 318
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 3 cut(s) 229, 395, 398
ApoI RAATTY 1 cut(s) 167
Asp700I GAANNNNTTC 1 cut(s) 618
AspS9I GGNCC 2 cut(s) 345, 435
AsuC2I CCSGG 2 cut(s) 175, 176
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 1 cut(s) 435
BamHI GGATCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 83, 432
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 3 cut(s) 241, 385, 407
BccI CCATC 1 cut(s) 128
BceAI ACGGC 3 cut(s) 177, 506, 523
BciT130I CCWGG 1 cut(s) 124
BcnI CCSGG 2 cut(s) 175, 176
BcoDI GTCTC 2 cut(s) 576, 634
BfaI CTAG 1 cut(s) 216
BisI GCNGC 3 cut(s) 230, 396, 399
BlsI GCNGC 3 cut(s) 231, 397, 400
Bme1390I CCNGG 3 cut(s) 124, 175, 176
Bme18I GGWCC 1 cut(s) 435
BmeRI GACNNNNNGTC 2 cut(s) 136, 433
BmeT110I CYCGRG 1 cut(s) 174
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 2 cut(s) 345, 435
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 3 cut(s) 124, 175, 176
BpiI GAAGAC 2 cut(s) 83, 432
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 2 cut(s) 411, 665
BpuEI CTTGAG 1 cut(s) 702
BpuMI CCSGG 2 cut(s) 175, 176
BsaJI CCNNGG 1 cut(s) 174
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 568
Bse1I ACTGG 1 cut(s) 648
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 1 cut(s) 324
BseLI CCNNNNNNNGG 2 cut(s) 267, 568
BseMII CTCAG 4 cut(s) 450, 534, 582, 600
BseNI ACTGG 1 cut(s) 648
BseRI GAGGAG 3 cut(s) 173, 430, 614
BseXI GCAGC 3 cut(s) 241, 385, 407
BsgI GTGCAG 1 cut(s) 384
BshFI GGCC 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 1 cut(s) 175
BslI CCNNNNNNNGG 2 cut(s) 267, 568
BsmAI GTCTC 2 cut(s) 576, 634
BsnI GGCC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 521
Bsp143I GATC 4 cut(s) 97, 178, 310, 363
BspACI CCGC 3 cut(s) 59, 164, 653
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 4 cut(s) 451, 533, 583, 599
BspLI GGNNCC 1 cut(s) 99
BspPI GGATC 4 cut(s) 92, 105, 186, 318
BsrI ACTGG 1 cut(s) 648
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 4 cut(s) 97, 178, 310, 363
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 1 cut(s) 206
Bst6I CTCTTC 2 cut(s) 200, 665
BstC8I GCNNGC 3 cut(s) 59, 703, 724
BstDEI CTNAG 4 cut(s) 459, 520, 586, 591
BstF5I GGATG 1 cut(s) 324
BstKTI GATC 4 cut(s) 100, 181, 313, 366
BstMAI GTCTC 2 cut(s) 576, 634
BstMBI GATC 4 cut(s) 97, 178, 310, 363
BstMWI GCNNNNNNNGC 3 cut(s) 170, 395, 516
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 2 cut(s) 88, 726
BstSCI CCNGG 3 cut(s) 122, 173, 174
BstV1I GCAGC 3 cut(s) 241, 385, 407
BstV2I GAAGAC 2 cut(s) 83, 432
BstX2I RGATCY 2 cut(s) 97, 178
BstYI RGATCY 2 cut(s) 97, 178
BsuRI GGCC 1 cut(s) 347
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 1 cut(s) 324
Cac8I GCNNGC 3 cut(s) 59, 703, 724
Cfr13I GGNCC 2 cut(s) 345, 435
Cfr9I CCCGGG 1 cut(s) 174
CviAII CATG 4 cut(s) 85, 342, 535, 723
DdeI CTNAG 4 cut(s) 459, 520, 586, 591
DpnI GATC 4 cut(s) 99, 180, 312, 365
DpnII GATC 4 cut(s) 97, 178, 310, 363
DriI GACNNNNNGTC 2 cut(s) 136, 433
Eam1104I CTCTTC 2 cut(s) 200, 665
Eam1105I GACNNNNNGTC 2 cut(s) 136, 433
EarI CTCTTC 2 cut(s) 200, 665
EciI GGCGGA 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 521
Eco32I GATATC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 122
EcoRV GATATC 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 521
FaeI CATG 4 cut(s) 88, 345, 538, 726
FaiI YATR 9 cut(s) 42, 86, 243, 319, 343, 530, 536, 579, 724
FatI CATG 4 cut(s) 84, 341, 534, 722
FauI CCCGC 2 cut(s) 66, 646
Fnu4HI GCNGC 3 cut(s) 230, 396, 399
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 521
Fsp4HI GCNGC 3 cut(s) 230, 396, 399
FspBI CTAG 1 cut(s) 216
GluI GCNGC 3 cut(s) 230, 396, 399
GsuI CTGGAG 2 cut(s) 411, 665
HaeIII GGCC 1 cut(s) 347
HapII CCGG 1 cut(s) 175
Hin1II CATG 4 cut(s) 88, 345, 538, 726
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 1 cut(s) 584
HpaII CCGG 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 3 cut(s) 296, 460, 523
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 2 cut(s) 116, 134
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 5 cut(s) 282, 374, 401, 549, 726
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 395, 516
HpyF3I CTNAG 4 cut(s) 459, 520, 586, 591
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 4 cut(s) 88, 345, 538, 726
Kzo9I GATC 4 cut(s) 97, 178, 310, 363
LmnI GCTCC 1 cut(s) 392
Lsp1109I GCAGC 3 cut(s) 241, 385, 407
MaeI CTAG 1 cut(s) 216
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 3 cut(s) 206, 320, 376
MalI GATC 4 cut(s) 99, 180, 312, 365
MboI GATC 4 cut(s) 97, 178, 310, 363
MboII GAAGA 6 cut(s) 88, 187, 345, 437, 617, 682
MflI RGATCY 2 cut(s) 97, 178
MhlI GDGCHC 1 cut(s) 521
MluCI AATT 2 cut(s) 167, 687
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 15
MnlI CCTC 9 cut(s) 88, 194, 448, 451, 592, 595, 640, 654, 660
MroXI GAANNNNTTC 1 cut(s) 618
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 2 cut(s) 398, 590
MspI CCGG 1 cut(s) 175
MspR9I CCNGG 3 cut(s) 124, 175, 176
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 3 cut(s) 170, 395, 516
NciI CCSGG 2 cut(s) 175, 176
NdeII GATC 4 cut(s) 97, 178, 310, 363
NlaIII CATG 4 cut(s) 88, 345, 538, 726
NlaIV GGNNCC 1 cut(s) 99
NmuCI GTSAC 1 cut(s) 320
NspI RCATGY 2 cut(s) 88, 726
PaeI GCATGC 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 618
PflMI CCANNNNNTGG 1 cut(s) 568
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 3 cut(s) 231, 397, 400
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
PpuMI RGGWCCY 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 521
Psp5II RGGWCCY 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 2 cut(s) 345, 435
PspPPI RGGWCCY 1 cut(s) 435
PsuI RGATCY 2 cut(s) 97, 178
PvuII CAGCTG 2 cut(s) 398, 590
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 1 cut(s) 521
SatI GCNGC 3 cut(s) 230, 396, 399
Sau3AI GATC 4 cut(s) 97, 178, 310, 363
Sau96I GGNCC 2 cut(s) 345, 435
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 3 cut(s) 124, 175, 176
SduI GDGCHC 1 cut(s) 521
SinI GGWCC 1 cut(s) 435
SmaI CCCGGG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
SphI GCATGC 1 cut(s) 726
Sse9I AATT 2 cut(s) 167, 687
SsiI CCGC 3 cut(s) 59, 164, 653
SspMI CTAG 1 cut(s) 216
SstI GAGCTC 1 cut(s) 521
StyD4I CCNGG 3 cut(s) 122, 173, 174
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 1 cut(s) 117
TasI AATT 2 cut(s) 167, 687
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 3 cut(s) 229, 395, 398
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 4 cut(s) 438, 438, 551, 627
TspGWI ACGGA 2 cut(s) 122, 317
TspMI CCCGGG 1 cut(s) 174
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 1 cut(s) 167
XceI RCATGY 2 cut(s) 88, 726
XmaI CCCGGG 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.