FvH4_6g27601

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010038
Physical Location & Seq
Forward (+)
21252701 .. 21257176
4476 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27601.t1

Sequence Viewer

Length: 1206 bp
ATGGCAGCCCTGAAGCTTCTCTTCTCCTCGGTTCGCCGTCACTCCCGTCTCCACCCCTCTCCCTTTCTCCGCTCCCTCTCCTCCGAATCCGATCAAGCTCCTCCTCCACCCCTTAAACCCCACCACCCCCTTCCGATCGAACCCGTTTCCTACGCTCCCAAACCCAAACCCGACCCGGCTCCGTCTCCGCAATCAGCACAACCTCAACCGCCGCCGCCGTCGGACCTTCGCCGGGACTCTGCCGGCGGGGAGTCGGTTCGGCCGTGGAGCCGGGAAGACGCGCGGTTCGTGAAGGACGGGCCGTCGATAACGCCGGTGTCGTATCCGGCGCGGGTGGCTCCGTTGCCGGAAGATAAGAAAGCGGAGGAGGGCGGCGGAGGTGCGAGGGAGGAGGAGTTGGAGAGAGAGAAGCAGAGGATCGAGCAGGCGAAGAGGAGGGCTTTTAGGTTGGAGGACAGATTGGTGGTTCCTTTTCCGACGTTTATTAAGGTGGAGAAGAAGGAGAGGAAGCCCATTCTTGATTTGATGGACGCAATTCGACAAGTCAAGGTCAGTGTGGCTACTGCCAAGTGCAACTTTGATGAAACTGTTGAAGCTCATGTACAGTTGGGTATCGATGCCAAGCGAAATGCGGTTCGTGGCAACATGACGTTGCCCCATGGTAGCGGAAAGGTAGTCAGGGTCGCTTTCTTTGCTGAAGGTGCAGATGCAGATGAAGCTAGAGCTGCTGGAGCAGATATTGTTGGCGGCGTGGACCTTGTGGAGGAAATTGCAAGCAGTCGCAAACTCAACGTTGACAAGTGTTTTGCAACAAATGAAATGGTTATGCGTCTAGCGAAGATAGCAAGAATTCTTAGAGAGCGTGGTTTAATGCCTGACCGGAAACTAGGTACTGTGACTAATGACATTGTTGGGGCACTACAAAAAGTAAGACAGGGACACATTGAGTATAAAATGGACAGAACATCAATCGTACATGTTGGGCTTGGAAAGGTAAGCTTTACAGAGGATAACCTGCGTGAGAATGTTGGTGCAATTATGAGTTCTCTTTTGCTGGCAAAGCCTACTGGTTTAAAAAAGTCTTCCAAATATGCTGGGTATATCAACTCCTTCCACATATGTAGCACAATGGGATCTGGATTTCCAGTTTCAATACAGTCATTATCCAAAGCTGCAGATAACTACAATAAGATGCACCTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L1

Protein Analysis

402

Amino Acids

44.05

Weight (kDa)

9.6

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 187 - 378 3.1e-45 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1023
AccBSI CCGCTC 1 cut(s) 72
AccII CGCG 3 cut(s) 281, 283, 331
AclI AACGTT 1 cut(s) 792
AclWI GGATC 2 cut(s) 425, 1141
AcoI YGGCCR 1 cut(s) 260
AcsI RAATTY 1 cut(s) 849
AcuI CTGAAG 2 cut(s) 32, 717
AfaI GTAC 3 cut(s) 603, 892, 975
AfiI CCNNNNNNNGG 2 cut(s) 232, 763
AflIII ACRYGT 1 cut(s) 976
AgsI TTSAA 2 cut(s) 593, 1152
AhdI GACNNNNNGTC 1 cut(s) 301
AluBI AGCT 7 cut(s) 16, 98, 596, 719, 725, 999, 1172
AluI AGCT 7 cut(s) 16, 98, 596, 719, 725, 999, 1172
Alw26I GTCTC 2 cut(s) 53, 189
AlwI GGATC 2 cut(s) 425, 1141
AoxI GGCC 2 cut(s) 260, 299
ApeKI GCWGC 3 cut(s) 5, 725, 1172
ApoI RAATTY 1 cut(s) 849
ArsI GACNNNNNNTTYG 4 cut(s) 269, 301, 788, 820
AspLEI GCGC 2 cut(s) 283, 331
AspS9I GGNCC 3 cut(s) 223, 299, 754
AsuC2I CCSGG 3 cut(s) 176, 233, 272
AvaII GGWCC 2 cut(s) 223, 754
BaeGI GKGCMC 1 cut(s) 919
BarI GAAGNNNNNNTAC 2 cut(s) 585, 617
BbsI GAAGAC 2 cut(s) 282, 1074
BbvI GCAGC 3 cut(s) 17, 712, 1159
BccI CCATC 1 cut(s) 520
BceAI ACGGC 4 cut(s) 21, 202, 247, 286
BciVI GTATCC 1 cut(s) 333
BcnI CCSGG 3 cut(s) 176, 233, 272
BcoDI GTCTC 2 cut(s) 53, 189
BfaI CTAG 3 cut(s) 720, 833, 887
BfmI CTRYAG 1 cut(s) 1173
BfuAI ACCTGC 1 cut(s) 1023
BfuI GTATCC 1 cut(s) 333
BisI GCNGC 7 cut(s) 6, 212, 215, 373, 726, 748, 1173
BlsI GCNGC 7 cut(s) 7, 213, 216, 374, 727, 749, 1174
Bme1390I CCNGG 3 cut(s) 176, 233, 272
Bme18I GGWCC 2 cut(s) 223, 754
BmeRI GACNNNNNGTC 1 cut(s) 301
BmgT120I GGNCC 3 cut(s) 223, 299, 754
BmiI GGNNCC 4 cut(s) 180, 269, 339, 468
BmrFI CCNGG 3 cut(s) 176, 233, 272
BmsI GCATC 3 cut(s) 607, 697, 1182
BpiI GAAGAC 2 cut(s) 282, 1074
BpmI CTGGAG 1 cut(s) 750
BpuEI CTTGAG 1 cut(s) 1184
BpuMI CCSGG 3 cut(s) 176, 233, 272
Bsa29I ATCGAT 1 cut(s) 615
BsaJI CCNNGG 3 cut(s) 27, 263, 658
BsaWI WCCGGW 1 cut(s) 879
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 763
Bse118I RCCGGY 2 cut(s) 242, 313
Bse1I ACTGG 2 cut(s) 1072, 1145
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 3 cut(s) 27, 263, 658
BseLI CCNNNNNNNGG 2 cut(s) 232, 763
BseNI ACTGG 2 cut(s) 1072, 1145
BseRI GAGGAG 8 cut(s) 16, 70, 90, 93, 380, 404, 407, 448
BseSI GKGCMC 1 cut(s) 919
BseX3I CGGCCG 1 cut(s) 260
BseXI GCAGC 3 cut(s) 17, 712, 1159
BseYI CCCAGC 1 cut(s) 1094
BsgI GTGCAG 1 cut(s) 723
Bsh1236I CGCG 3 cut(s) 281, 283, 331
Bsh1285I CGRYCG 2 cut(s) 138, 263
BshFI GGCC 2 cut(s) 262, 301
BshVI ATCGAT 1 cut(s) 615
BsiEI CGRYCG 2 cut(s) 138, 263
BsiSI CCGG 8 cut(s) 176, 232, 243, 271, 314, 326, 347, 880
BslFI GGGAC 2 cut(s) 248, 951
BslI CCNNNNNNNGG 2 cut(s) 232, 763
BsmAI GTCTC 2 cut(s) 53, 189
BsmBI CGTCTC 2 cut(s) 53, 189
BsmFI GGGAC 2 cut(s) 248, 951
BsnI GGCC 2 cut(s) 262, 301
Bsp1286I GDGCHC 1 cut(s) 919
Bsp1407I TGTACA 1 cut(s) 601
Bsp143I GATC 4 cut(s) 91, 135, 417, 1133
Bsp19I CCATGG 1 cut(s) 658
BspANI GGCC 2 cut(s) 262, 301
BspDI ATCGAT 1 cut(s) 615
BspFNI CGCG 3 cut(s) 281, 283, 331
BspLI GGNNCC 4 cut(s) 180, 269, 339, 468
BspMAI CTGCAG 1 cut(s) 1177
BspMI ACCTGC 1 cut(s) 1023
BspPI GGATC 2 cut(s) 425, 1141
BsrBI CCGCTC 1 cut(s) 72
BsrFI RCCGGY 2 cut(s) 242, 313
BsrGI TGTACA 1 cut(s) 601
BsrI ACTGG 2 cut(s) 1072, 1145
BssAI RCCGGY 2 cut(s) 242, 313
BssECI CCNNGG 3 cut(s) 27, 263, 658
BssMI GATC 4 cut(s) 91, 135, 417, 1133
BssT1I CCWWGG 1 cut(s) 658
Bst4CI ACNGT 4 cut(s) 589, 606, 895, 1158
Bst6I CTCTTC 2 cut(s) 26, 425
BstAUI TGTACA 1 cut(s) 601
BstC8I GCNNGC 4 cut(s) 244, 426, 775, 1056
BstDEI CTNAG 1 cut(s) 854
BstDSI CCRYGG 2 cut(s) 263, 658
BstENI CCTNNNNNAGG 1 cut(s) 761
BstFNI CGCG 3 cut(s) 281, 283, 331
BstHHI GCGC 2 cut(s) 283, 331
BstKTI GATC 4 cut(s) 94, 138, 420, 1136
BstMAI GTCTC 2 cut(s) 53, 189
BstMBI GATC 4 cut(s) 91, 135, 417, 1133
BstMCI CGRYCG 2 cut(s) 138, 263
BstMWI GCNNNNNNNGC 8 cut(s) 335, 692, 701, 716, 725, 731, 842, 1060
BstNSI RCATGY 1 cut(s) 980
BstSCI CCNGG 3 cut(s) 174, 231, 270
BstSFI CTRYAG 1 cut(s) 1173
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 3 cut(s) 281, 283, 331
BstV1I GCAGC 3 cut(s) 17, 712, 1159
BstV2I GAAGAC 2 cut(s) 282, 1074
BstX2I RGATCY 1 cut(s) 1133
BstYI RGATCY 1 cut(s) 1133
BstZI CGGCCG 1 cut(s) 260
Bsu15I ATCGAT 1 cut(s) 615
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 2 cut(s) 262, 301
BsuTUI ATCGAT 1 cut(s) 615
BtgI CCRYGG 2 cut(s) 263, 658
BtsIMutI CAGTG 1 cut(s) 559
BveI ACCTGC 1 cut(s) 1023
Cac8I GCNNGC 4 cut(s) 244, 426, 775, 1056
CfoI GCGC 2 cut(s) 283, 331
Cfr10I RCCGGY 2 cut(s) 242, 313
Cfr13I GGNCC 3 cut(s) 223, 299, 754
ClaI ATCGAT 1 cut(s) 615
CseI GACGC 3 cut(s) 287, 539, 818
Csp6I GTAC 3 cut(s) 602, 891, 974
CviAII CATG 4 cut(s) 599, 646, 659, 977
CviQI GTAC 3 cut(s) 602, 891, 974
DdeI CTNAG 1 cut(s) 854
DpnI GATC 4 cut(s) 93, 137, 419, 1135
DpnII GATC 4 cut(s) 91, 135, 417, 1133
DraI TTTAAA 1 cut(s) 1074
DriI GACNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 260
EagI CGGCCG 1 cut(s) 260
Eam1104I CTCTTC 2 cut(s) 26, 425
Eam1105I GACNNNNNGTC 1 cut(s) 301
EarI CTCTTC 2 cut(s) 26, 425
EciI GGCGGA 1 cut(s) 390
EclXI CGGCCG 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 658
Eco47I GGWCC 2 cut(s) 223, 754
Eco52I CGGCCG 1 cut(s) 260
Eco57I CTGAAG 2 cut(s) 32, 717
EcoNI CCTNNNNNAGG 1 cut(s) 761
EcoRI GAATTC 1 cut(s) 849
EcoT14I CCWWGG 1 cut(s) 658
ErhI CCWWGG 1 cut(s) 658
Esp3I CGTCTC 2 cut(s) 53, 189
FaeI CATG 4 cut(s) 602, 649, 662, 980
FalI AAGNNNNNCTT 5 cut(s) 37, 560, 592, 983, 1015
FaqI GGGAC 2 cut(s) 248, 951
FatI CATG 4 cut(s) 598, 645, 658, 976
FauI CCCGC 2 cut(s) 239, 324
FauNDI CATATG 1 cut(s) 1118
Fnu4HI GCNGC 7 cut(s) 6, 212, 215, 373, 726, 748, 1173
Fsp4HI GCNGC 7 cut(s) 6, 212, 215, 373, 726, 748, 1173
FspBI CTAG 3 cut(s) 720, 833, 887
GlaI GCGC 2 cut(s) 282, 330
GluI GCNGC 7 cut(s) 6, 212, 215, 373, 726, 748, 1173
GsaI CCCAGC 1 cut(s) 1098
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 2 cut(s) 262, 301
HapII CCGG 8 cut(s) 176, 232, 243, 271, 314, 326, 347, 880
HgaI GACGC 3 cut(s) 287, 539, 818
HhaI GCGC 2 cut(s) 283, 331
Hin1II CATG 4 cut(s) 602, 649, 662, 980
Hin6I GCGC 2 cut(s) 281, 329
HinP1I GCGC 2 cut(s) 281, 329
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 2 cut(s) 14, 997
HinfI GANTC 3 cut(s) 86, 236, 251
HpaII CCGG 8 cut(s) 176, 232, 243, 271, 314, 326, 347, 880
Hpy166II GTNNAC 2 cut(s) 754, 796
Hpy188I TCNGA 5 cut(s) 85, 91, 135, 223, 477
Hpy188III TCNNGA 3 cut(s) 289, 518, 1137
Hpy8I GTNNAC 2 cut(s) 754, 796
Hpy99I CGWCG 3 cut(s) 223, 307, 481
HpyAV CCTTC 6 cut(s) 140, 236, 286, 493, 692, 1120
HpyCH4III ACNGT 4 cut(s) 589, 606, 895, 1158
HpyCH4IV ACGT 3 cut(s) 479, 650, 792
HpyCH4V TGCA 8 cut(s) 573, 704, 710, 773, 809, 1034, 1175, 1195
HpyF10VI GCNNNNNNNGC 8 cut(s) 335, 692, 701, 716, 725, 731, 842, 1060
HpyF3I CTNAG 1 cut(s) 854
HpySE526I ACGT 3 cut(s) 479, 650, 792
Hsp92II CATG 4 cut(s) 602, 649, 662, 980
HspAI GCGC 2 cut(s) 281, 329
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Kzo9I GATC 4 cut(s) 91, 135, 417, 1133
LmnI GCTCC 7 cut(s) 77, 103, 160, 184, 267, 343, 731
Lsp1109I GCAGC 3 cut(s) 17, 712, 1159
LweI GCATC 3 cut(s) 607, 697, 1182
MaeI CTAG 3 cut(s) 720, 833, 887
MaeII ACGT 3 cut(s) 479, 650, 792
MaeIII GTNAC 2 cut(s) 38, 895
MalI GATC 4 cut(s) 93, 137, 419, 1135
MbiI CCGCTC 1 cut(s) 72
MboI GATC 4 cut(s) 91, 135, 417, 1133
MboII GAAGA 7 cut(s) 13, 287, 362, 442, 508, 850, 1074
MflI RGATCY 1 cut(s) 1133
MhlI GDGCHC 1 cut(s) 919
MluCI AATT 4 cut(s) 534, 768, 849, 1035
MlyI GAGTC 2 cut(s) 230, 260
MmeI TCCRAC 4 cut(s) 201, 378, 429, 500
MroNI GCCGGC 1 cut(s) 242
MseI TTAA 4 cut(s) 114, 486, 869, 1073
MspI CCGG 8 cut(s) 176, 232, 243, 271, 314, 326, 347, 880
MspR9I CCNGG 3 cut(s) 176, 233, 272
MvnI CGCG 3 cut(s) 281, 283, 331
MwoI GCNNNNNNNGC 8 cut(s) 335, 692, 701, 716, 725, 731, 842, 1060
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 3 cut(s) 176, 233, 272
NcoI CCATGG 1 cut(s) 658
NdeI CATATG 1 cut(s) 1118
NdeII GATC 4 cut(s) 91, 135, 417, 1133
NgoMIV GCCGGC 1 cut(s) 242
NlaIII CATG 4 cut(s) 602, 649, 662, 980
NlaIV GGNNCC 4 cut(s) 180, 269, 339, 468
NmuCI GTSAC 2 cut(s) 38, 895
NspI RCATGY 1 cut(s) 980
PciI ACATGT 1 cut(s) 976
PcsI WCGNNNNNNNCGW 3 cut(s) 260, 285, 317
PdiI GCCGGC 1 cut(s) 244
PfeI GAWTC 1 cut(s) 86
PkrI GCNGC 7 cut(s) 7, 213, 216, 374, 727, 749, 1174
Ple19I CGATCG 1 cut(s) 138
PleI GAGTC 2 cut(s) 230, 259
PpsI GAGTC 2 cut(s) 230, 259
PscI ACATGT 1 cut(s) 976
Psp1406I AACGTT 1 cut(s) 792
PspFI CCCAGC 1 cut(s) 1094
PspN4I GGNNCC 4 cut(s) 180, 269, 339, 468
PspPI GGNCC 3 cut(s) 223, 299, 754
PstI CTGCAG 1 cut(s) 1177
PsuI RGATCY 1 cut(s) 1133
PvuI CGATCG 1 cut(s) 138
RsaI GTAC 3 cut(s) 603, 892, 975
RsaNI GTAC 3 cut(s) 602, 891, 974
SaqAI TTAA 4 cut(s) 114, 486, 869, 1073
SatI GCNGC 7 cut(s) 6, 212, 215, 373, 726, 748, 1173
Sau3AI GATC 4 cut(s) 91, 135, 417, 1133
Sau96I GGNCC 3 cut(s) 223, 299, 754
SchI GAGTC 2 cut(s) 230, 260
ScrFI CCNGG 3 cut(s) 176, 233, 272
SduI GDGCHC 1 cut(s) 919
SfaNI GCATC 3 cut(s) 607, 697, 1182
SfcI CTRYAG 1 cut(s) 1173
SgrAI CRCCGGYG 1 cut(s) 313
SinI GGWCC 2 cut(s) 223, 754
SmlI CTYRAG 1 cut(s) 1199
SmoI CTYRAG 1 cut(s) 1199
Sse9I AATT 4 cut(s) 534, 768, 849, 1035
SspMI CTAG 3 cut(s) 720, 833, 887
StyD4I CCNGG 3 cut(s) 174, 231, 270
StyI CCWWGG 1 cut(s) 658
TaaI ACNGT 4 cut(s) 589, 606, 895, 1158
TaiI ACGT 3 cut(s) 482, 653, 795
TaqI TCGA 5 cut(s) 138, 305, 420, 538, 615
TasI AATT 4 cut(s) 534, 768, 849, 1035
TatI WGTACW 1 cut(s) 601
TauI GCSGC 4 cut(s) 214, 217, 375, 750
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 4 cut(s) 114, 486, 869, 1073
Tru9I TTAA 4 cut(s) 114, 486, 869, 1073
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 2 cut(s) 38, 895
TseI GCWGC 3 cut(s) 5, 725, 1172
Tsp45I GTSAC 2 cut(s) 38, 895
TspDTI ATGAA 3 cut(s) 597, 729, 831
TspGWI ACGGA 2 cut(s) 171, 330
TspRI CASTG 1 cut(s) 559
VpaK11BI GGWCC 2 cut(s) 223, 754
XagI CCTNNNNNAGG 1 cut(s) 761
XapI RAATTY 1 cut(s) 849
XceI RCATGY 1 cut(s) 980
XspI CTAG 3 cut(s) 720, 833, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.