Rorug02G0291100

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000393
Physical Location & Seq
Forward (+)
31988544 .. 31993783
5240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0291100.1

Sequence Viewer

Length: 2709 bp
ATGGAAGCAAGAGGGGCAGTTCTACCAATTTTCTATTCTGTGGAGCCCTCAGATGTTGGAAAACAATTGGGAACTTTTGGACAAGGCTTCACTAAACTTGAAGAAAAGTTTAAGAATGACGAAGATCAGGTGGTGCGGTGGAGAGCTGCTTTAAGGGAAGTGTCGAAGATCAAAGGGTGGACTTCAAAGGATAGATCTGAGCCAGAGCTCATCAAAGAAATTGTTGAAAAGGTACGAGACAAAGTACCTCTGGTATTGTTGGAGCCTGCAGAAAAGCTAGTTGGAATTGATTCAAGATTAGAGCAACTAGATTCTCTTTTGGATACAGGATCAAATGATGTTCATTTTATAGGGATATGGGGGTTAGGGGGGATAGGAAAGACAACCCTTGCTAAAAGAGCTTATGAGAGGATGTATACTAAATTTGAACACAAAAGCTTTCTTGACAGAGTTGGAGAGGAAGATGTTCTCAAAAGAGATGATCTAGCTAATCTAAAAAGAAAACTCTCCAAGGACCTTATGAAGAGAGACAAAAGAGATTGGAACGAGCATGAAGAAGAGAGATTGAGACATTTCCTACTTCAGAAAAAGGTTCTTCTCATTCTTGATGATGTGGACCATATAAATCAGTTAGAAAAGTTATGTGGACACACAAATTGGTTTCATCATGGGAGTAGAATTATCATTACGACTAGAGATAAACATTTTCTAATATCACATGGTGTGAAAAGGATATATAAGTTGCCGGGGTTGAATGAAGATGATGCTCTTCAACTTTTTAGCTGGAAAGCCTTTGGAAGAGATTATCCGGATGATAATTATGAACATCTGTGTAAAAGGTTTGTTGATTATGCCAATGGTCTTCCCCTAGCTCTTAAGGTCTTGGGATCACATTTGCATGGAAGAGGTGAAGAGGCGTGGCACAGCGAATTGGGTAAACTGTTGACAGACAATTTAAATGGAGAGATTATGAAAATACTGAAAAGCAGTTATGATGGATTAGATGAGCAGCAGAAAAATATTTTCCTTGATATTGCATGTTTTTTCAAAGGAATGTGCAAAGATAGGGTAGTTGAAGTACTTGATGGTTGTGGTTTTTATCCAGGCATTGACATAGATGTCCTTAGCAAGAGGTCTCTTATATCGATTTCCAATAACATTGTTTGGATGCATGATTTTTCACAAAAAATGGGTCAATCAATTGTTCTTCAACAATCTCAAGAAGCTGGTGAACGTAGCAGGTTGTGGCGTTCTGAGGACATCATTCATGTTTTAAGAAATAATACGGGGACTGCAGCAGTTAAAGGAATTGCCCTAGACTTTCCTGAGTCGAAAGGGGTACAGTGCCATCACAATGCCTTTTCTAAGATGTTTAATCTTAAATTCCTCAAACTTCATAATGTGCTCCCTTCCAAAGACCTCAGATGTCTTCCTCAGTCATTACGATTTTTTGAATGGAGAGGTTATTCTTTAAAAAATCTCCCACCAGATTTCAAACCGGATGGGCTTGTTGAACTCAGCATGTGCCATAGTAGCATTGAACAACTTTGGAATGAAGTAAAGAAGTGTGACAGCTTAAAAGTCATGGAACTTCGTCATTCAAAAAGTTTAATAAGAACGCCAGATTTCAAAGAGATCCAAAATCTTGAAAGATTAGATCTCGAGGGGTGCGAGAGTCTAGGTGAGATCCACCCATCTATAGGAGTTCTCAAAAAGCTCAAGTTCTTGAATCTTAAGGACTGCACAAGTCTTGTGAGCCTTCCACCTAAGATTGAAATGGTCATGCTTGAAACTCTTATTCTCTCTGGTTGCTCAAGTGTTAGAACCATTCCTGAATTCGGGGGTTGTATGAATCTATTGTTGAAACTTTCTTTAGATGGGACTGCTATTGAAAGCATACCTTCGTCAATAGAACATCTGAGCAGCCTTTCATCATTGGATTTAAGAGATTGCATAAATCTGAAGTGTCTTCCAAGTACCATTGGCAGTTTGAAGGTCCTTGAAAGCCTTCATGTTTCTGGATGTTCAAAACTAGCTGAATTGCCACAGAGTCTTGGGAAATTAGGGAGCCTGGCCGAGATTGATGCAAGTGGAACAGCTATAAAAAAGTTATCACCTCTTCCAAAGAATCTTACGTCATTAATTTTTCGTGGAACGCAAGGGCAATCATCATATCTGTCCCTTTCTCGATTATTTCCAATGAAGAGTTCAATCTTGCCTCCTCTATCAGGGCTGCTTTGCCTACAGGAGTTAGATATAAGTAACAGAAATCTTTGTGCAGAAGCAATACCCGGTGATATTGGCTGCTTATCTTCTTTGTTATCGCTGAACTTGAGTGGAAACGATTTCATTAGCCTTCCTACAGGAATTAGCAAACTCTCCAAGCTGGAGAATTTGTACTTGAGCGATTGCAGCAAACTTCAACAACTTCCACTACTCTCATCAGATAAAAATTTGGAAGTAGTTGCTGATGGTTGTACTTCACTGGAGGAAGTGCAATACCCATCAAATTTGAGGAGATTGAATCGCTTATTGTTCAATTTCATCAACTGCTCTAGAGCGGTTCACAAAGAAAGCTTCCATCATTACACATTGACTATGCTCCAAAGATACCTTAAGGTGCTCTCTCTCTCTCTCGCCCCCCCCCCCCCCCCTCCCTGCCTCTATAACATCACAGTTTCTAATGCACACTCTCTCGACATTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

902

Amino Acids

101.66

Weight (kDa)

7.53

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 6 - 87 8.9e-14 TIR domain
NB-ARC PF00931 99 - 266 6.8e-21 NB-ARC domain
ATPase_2 PF01637 100 - 212 8.1e-06 ATPase domain predominantly from Archaea
WHD_ROQ1 PF23282 336 - 406 1e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 595 - 684 4.9e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 623 - 699 2.8e-11 Leucine-rich repeat region
LRR_14 PF23598 739 - 871 3.1e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1118
Acc36I ACCTGC 1 cut(s) 1230
AccBSI CCGCTC 1 cut(s) 2561
AccI GTMKAC 1 cut(s) 416
AccIII TCCGGA 1 cut(s) 808
AciI CCGC 2 cut(s) 136, 2561
AclWI GGATC 4 cut(s) 337, 895, 1630, 1681
AcoI YGGCCR 1 cut(s) 2073
AcsI RAATTY 6 cut(s) 422, 1382, 1835, 2392, 2452, 2509
AcuI CTGAAG 2 cut(s) 566, 1982
AdeI CACNNNGTG 1 cut(s) 722
AfaI GTAC 7 cut(s) 234, 246, 1080, 1341, 1978, 2399, 2479
AfiI CCNNNNNNNGG 3 cut(s) 1700, 1838, 2228
AflII CTTAAG 3 cut(s) 875, 1733, 2615
AjnI CCWGG 2 cut(s) 1102, 2070
AjuI GAANNNNNNNTTGG 6 cut(s) 19, 51, 264, 296, 2191, 2223
Alw21I GWGCWC 3 cut(s) 210, 1407, 2625
Alw26I GTCTC 4 cut(s) 231, 522, 562, 1140
AlwI GGATC 4 cut(s) 337, 895, 1630, 1681
Ama87I CYCGRG 1 cut(s) 1661
Aor13HI TCCGGA 1 cut(s) 808
AoxI GGCC 1 cut(s) 2073
ApeKI GCWGC 7 cut(s) 146, 1009, 1295, 1923, 2233, 2304, 2412
ApoI RAATTY 6 cut(s) 422, 1382, 1835, 2392, 2452, 2509
ArsI GACNNNNNNTTYG 2 cut(s) 1177, 1209
AseI ATTAAT 1 cut(s) 2141
Asp700I GAANNNNTTC 3 cut(s) 289, 465, 2007
AspS9I GGNCC 3 cut(s) 514, 616, 1996
AsuC2I CCSGG 2 cut(s) 747, 2292
AsuHPI GGTGA 5 cut(s) 920, 1241, 1694, 2106, 2306
AvaI CYCGRG 1 cut(s) 1661
AvaII GGWCC 3 cut(s) 514, 616, 1996
BanII GRGCYC 2 cut(s) 48, 210
BbsI GAAGAC 3 cut(s) 854, 1421, 1961
Bbv12I GWGCWC 3 cut(s) 210, 1407, 2625
BbvI GCAGC 7 cut(s) 133, 1021, 1307, 1935, 2220, 2291, 2424
BccI CCATC 9 cut(s) 989, 1079, 1356, 1496, 1702, 1871, 2465, 2512, 2589
BcgI CGANNNNNNTGC 2 cut(s) 2066, 2100
BciT130I CCWGG 2 cut(s) 1104, 2072
BciVI GTATCC 1 cut(s) 316
BcnI CCSGG 2 cut(s) 747, 2292
BcoDI GTCTC 4 cut(s) 231, 522, 562, 1140
BfaI CTAG 9 cut(s) 278, 308, 485, 693, 869, 1316, 1679, 2033, 2556
BfmI CTRYAG 5 cut(s) 267, 1293, 1698, 2243, 2361
BfrI CTTAAG 3 cut(s) 875, 1733, 2615
BfuAI ACCTGC 1 cut(s) 1230
BfuI GTATCC 1 cut(s) 316
BglII AGATCT 2 cut(s) 194, 1657
BisI GCNGC 7 cut(s) 147, 1010, 1296, 1924, 2234, 2305, 2413
BlsI GCNGC 7 cut(s) 148, 1011, 1297, 1925, 2235, 2306, 2414
BmcAI AGTACT 1 cut(s) 1080
Bme1390I CCNGG 4 cut(s) 747, 1104, 2072, 2292
Bme18I GGWCC 3 cut(s) 514, 616, 1996
BmeT110I CYCGRG 1 cut(s) 1661
BmgT120I GGNCC 3 cut(s) 514, 616, 1996
BmiI GGNNCC 3 cut(s) 45, 264, 2069
BmrFI CCNGG 4 cut(s) 747, 1104, 2072, 2292
BmsI GCATC 3 cut(s) 754, 1158, 2074
BpiI GAAGAC 3 cut(s) 854, 1421, 1961
BpmI CTGGAG 2 cut(s) 2408, 2507
Bpu10I CCTNAGC 1 cut(s) 1124
BpuEI CTTGAG 5 cut(s) 1203, 1703, 1798, 2353, 2422
BpuMI CCSGG 2 cut(s) 747, 2292
Bsa29I ATCGAT 1 cut(s) 1145
BsaI GGTCTC 1 cut(s) 1140
BsaJI CCNNGG 2 cut(s) 510, 746
BsaWI WCCGGW 2 cut(s) 808, 1498
BsaXI ACNNNNNCTCC 2 cut(s) 2381, 2411
Bsc4I CCNNNNNNNGG 3 cut(s) 1700, 1838, 2228
Bse1I ACTGG 1 cut(s) 2490
BseAI TCCGGA 1 cut(s) 808
BseBI CCWGG 2 cut(s) 1104, 2072
BseCI ATCGAT 1 cut(s) 1145
BseDI CCNNGG 2 cut(s) 510, 746
BseGI GGATG 5 cut(s) 417, 817, 1173, 1507, 2027
BseLI CCNNNNNNNGG 3 cut(s) 1700, 1838, 2228
BseMII CTCAG 8 cut(s) 63, 189, 1245, 1317, 1435, 1448, 1531, 1910
BseNI ACTGG 1 cut(s) 2490
BseRI GAGGAG 2 cut(s) 2211, 2530
BseXI GCAGC 7 cut(s) 133, 1021, 1307, 1935, 2220, 2291, 2424
BsgI GTGCAG 2 cut(s) 1726, 2298
BshFI GGCC 1 cut(s) 2075
BshVI ATCGAT 1 cut(s) 1145
BsiHKAI GWGCWC 3 cut(s) 210, 1407, 2625
BsiHKCI CYCGRG 1 cut(s) 1661
BsiSI CCGG 4 cut(s) 746, 809, 1499, 2292
BslFI GGGAC 3 cut(s) 1303, 1894, 2164
BslI CCNNNNNNNGG 3 cut(s) 1700, 1838, 2228
BsmAI GTCTC 4 cut(s) 231, 522, 562, 1140
BsmFI GGGAC 3 cut(s) 1303, 1894, 2164
BsnI GGCC 1 cut(s) 2075
Bso31I GGTCTC 1 cut(s) 1140
BsoBI CYCGRG 1 cut(s) 1661
Bsp1286I GDGCHC 4 cut(s) 48, 210, 1407, 2625
Bsp13I TCCGGA 1 cut(s) 808
Bsp143I GATC 9 cut(s) 124, 168, 194, 329, 481, 887, 1635, 1657, 1686
BspACI CCGC 2 cut(s) 136, 2561
BspANI GGCC 1 cut(s) 2075
BspCNI CTCAG 8 cut(s) 62, 190, 1246, 1318, 1434, 1447, 1530, 1911
BspDI ATCGAT 1 cut(s) 1145
BspEI TCCGGA 1 cut(s) 808
BspLI GGNNCC 3 cut(s) 45, 264, 2069
BspMAI CTGCAG 2 cut(s) 271, 1297
BspMI ACCTGC 1 cut(s) 1230
BspPI GGATC 4 cut(s) 337, 895, 1630, 1681
BspQI GCTCTTC 1 cut(s) 774
BspTI CTTAAG 3 cut(s) 875, 1733, 2615
BspTNI GGTCTC 1 cut(s) 1140
BsrBI CCGCTC 1 cut(s) 2561
BsrI ACTGG 1 cut(s) 2490
BssECI CCNNGG 2 cut(s) 510, 746
BssMI GATC 9 cut(s) 124, 168, 194, 329, 481, 887, 1635, 1657, 1686
BssNAI GTATAC 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 510
Bst1107I GTATAC 1 cut(s) 417
Bst2UI CCWGG 2 cut(s) 1104, 2072
Bst4CI ACNGT 3 cut(s) 942, 1344, 2677
Bst6I CTCTTC 8 cut(s) 518, 552, 774, 793, 898, 906, 2124, 2198
BstAFI CTTAAG 3 cut(s) 875, 1733, 2615
BstC8I GCNNGC 1 cut(s) 267
BstENI CCTNNNNNAGG 1 cut(s) 2226
BstF5I GGATG 5 cut(s) 417, 817, 1173, 1507, 2027
BstKTI GATC 9 cut(s) 127, 171, 197, 332, 484, 890, 1638, 1660, 1689
BstMAI GTCTC 4 cut(s) 231, 522, 562, 1140
BstMBI GATC 9 cut(s) 124, 168, 194, 329, 481, 887, 1635, 1657, 1686
BstMWI GCNNNNNNNGC 4 cut(s) 14, 398, 1533, 2412
BstNI CCWGG 2 cut(s) 1104, 2072
BstNSI RCATGY 2 cut(s) 1041, 1525
BstSCI CCNGG 4 cut(s) 745, 1102, 2070, 2290
BstSFI CTRYAG 5 cut(s) 267, 1293, 1698, 2243, 2361
BstV1I GCAGC 7 cut(s) 133, 1021, 1307, 1935, 2220, 2291, 2424
BstV2I GAAGAC 3 cut(s) 854, 1421, 1961
BstX2I RGATCY 4 cut(s) 194, 1635, 1657, 1686
BstYI RGATCY 4 cut(s) 194, 1635, 1657, 1686
BstZ17I GTATAC 1 cut(s) 417
Bsu15I ATCGAT 1 cut(s) 1145
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 1 cut(s) 2075
BsuTUI ATCGAT 1 cut(s) 1145
BtsCI GGATG 5 cut(s) 417, 817, 1173, 1507, 2027
BtsIMutI CAGTG 2 cut(s) 1349, 2483
BveI ACCTGC 1 cut(s) 1230
Cac8I GCNNGC 1 cut(s) 267
Cfr13I GGNCC 3 cut(s) 514, 616, 1996
ClaI ATCGAT 1 cut(s) 1145
Csp6I GTAC 7 cut(s) 233, 245, 1079, 1340, 1977, 2398, 2478
CviQI GTAC 7 cut(s) 233, 245, 1079, 1340, 1977, 2398, 2478
DpnI GATC 9 cut(s) 126, 170, 196, 331, 483, 889, 1637, 1659, 1688
DpnII GATC 9 cut(s) 124, 168, 194, 329, 481, 887, 1635, 1657, 1686
DraI TTTAAA 2 cut(s) 957, 1473
DraIII CACNNNGTG 1 cut(s) 722
DrdI GACNNNNNNGTC 1 cut(s) 1118
DseDI GACNNNNNNGTC 1 cut(s) 1118
EaeI YGGCCR 1 cut(s) 2073
Eam1104I CTCTTC 8 cut(s) 518, 552, 774, 793, 898, 906, 2124, 2198
EarI CTCTTC 8 cut(s) 518, 552, 774, 793, 898, 906, 2124, 2198
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 510
Eco24I GRGCYC 2 cut(s) 48, 210
Eco31I GGTCTC 1 cut(s) 1140
Eco47I GGWCC 3 cut(s) 514, 616, 1996
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 2 cut(s) 566, 1982
Eco88I CYCGRG 1 cut(s) 1661
EcoICRI GAGCTC 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 2226
EcoO109I RGGNCCY 2 cut(s) 514, 1996
EcoRI GAATTC 1 cut(s) 1835
EcoRII CCWGG 2 cut(s) 1102, 2070
EcoT14I CCWWGG 1 cut(s) 510
EcoT22I ATGCAT 1 cut(s) 1173
EcoT38I GRGCYC 2 cut(s) 48, 210
ErhI CCWWGG 1 cut(s) 510
FalI AAGNNNNNCTT 2 cut(s) 1885, 1917
FaqI GGGAC 3 cut(s) 1303, 1894, 2164
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 7 cut(s) 147, 1010, 1296, 1924, 2234, 2305, 2413
FokI GGATG 5 cut(s) 424, 824, 1180, 1514, 2034
FriOI GRGCYC 2 cut(s) 48, 210
Fsp4HI GCNGC 7 cut(s) 147, 1010, 1296, 1924, 2234, 2305, 2413
FspBI CTAG 9 cut(s) 278, 308, 485, 693, 869, 1316, 1679, 2033, 2556
GluI GCNGC 7 cut(s) 147, 1010, 1296, 1924, 2234, 2305, 2413
GsuI CTGGAG 2 cut(s) 2408, 2507
HaeIII GGCC 1 cut(s) 2075
HapII CCGG 4 cut(s) 746, 809, 1499, 2292
HincII GTYRAC 1 cut(s) 945
HindII GTYRAC 1 cut(s) 945
HindIII AAGCTT 2 cut(s) 436, 2575
HinfI GANTC 9 cut(s) 290, 311, 1328, 1675, 1729, 1852, 2050, 2128, 2524
HpaII CCGG 4 cut(s) 746, 809, 1499, 2292
HphI GGTGA 5 cut(s) 920, 1241, 1694, 2106, 2306
Hpy166II GTNNAC 8 cut(s) 180, 417, 616, 647, 938, 945, 1232, 2566
Hpy188I TCNGA 8 cut(s) 52, 199, 585, 1255, 1424, 1920, 1962, 2446
Hpy8I GTNNAC 8 cut(s) 180, 417, 616, 647, 938, 945, 1232, 2566
HpyAV CCTTC 6 cut(s) 1419, 1769, 1911, 1987, 2018, 2366
HpyCH4III ACNGT 3 cut(s) 942, 1344, 2677
HpyCH4IV ACGT 2 cut(s) 1234, 2135
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 398, 1533, 2412
HpySE526I ACGT 2 cut(s) 1234, 2135
Kpn2I TCCGGA 1 cut(s) 808
Kzo9I GATC 9 cut(s) 124, 168, 194, 329, 481, 887, 1635, 1657, 1686
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 5 cut(s) 43, 262, 1410, 2067, 2607
Lsp1109I GCAGC 7 cut(s) 133, 1021, 1307, 1935, 2220, 2291, 2424
LweI GCATC 3 cut(s) 754, 1158, 2074
MaeI CTAG 9 cut(s) 278, 308, 485, 693, 869, 1316, 1679, 2033, 2556
MaeII ACGT 2 cut(s) 1234, 2135
MaeIII GTNAC 2 cut(s) 1568, 2261
MalI GATC 9 cut(s) 126, 170, 196, 331, 483, 889, 1637, 1659, 1688
MbiI CCGCTC 1 cut(s) 2561
MboI GATC 9 cut(s) 124, 168, 194, 329, 481, 887, 1635, 1657, 1686
MfeI CAATTG 2 cut(s) 65, 1200
MflI RGATCY 4 cut(s) 194, 1635, 1657, 1686
MhlI GDGCHC 4 cut(s) 48, 210, 1407, 2625
MlyI GAGTC 3 cut(s) 1337, 1684, 2059
MmeI TCCRAC 4 cut(s) 37, 240, 262, 433
Mph1103I ATGCAT 1 cut(s) 1173
MroI TCCGGA 1 cut(s) 808
MroXI GAANNNNTTC 3 cut(s) 289, 465, 2007
MslI CAYNNNNRTG 2 cut(s) 897, 1353
MspCI CTTAAG 3 cut(s) 875, 1733, 2615
MspI CCGG 4 cut(s) 746, 809, 1499, 2292
MspR9I CCNGG 4 cut(s) 747, 1104, 2072, 2292
MunI CAATTG 2 cut(s) 65, 1200
MvaI CCWGG 2 cut(s) 1104, 2072
MwoI GCNNNNNNNGC 4 cut(s) 14, 398, 1533, 2412
NciI CCSGG 2 cut(s) 747, 2292
NdeII GATC 9 cut(s) 124, 168, 194, 329, 481, 887, 1635, 1657, 1686
NlaIV GGNNCC 3 cut(s) 45, 264, 2069
NmeAIII GCCGAG 1 cut(s) 2101
NmuCI GTSAC 1 cut(s) 1568
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 2 cut(s) 1041, 1525
PaeR7I CTCGAG 1 cut(s) 1661
PciSI GCTCTTC 1 cut(s) 774
PcsI WCGNNNNNNNCGW 1 cut(s) 1668
PdmI GAANNNNTTC 3 cut(s) 289, 465, 2007
PfeI GAWTC 6 cut(s) 290, 311, 1729, 1852, 2128, 2524
PkrI GCNGC 7 cut(s) 148, 1011, 1297, 1925, 2235, 2306, 2414
PleI GAGTC 3 cut(s) 1336, 1683, 2058
PpsI GAGTC 3 cut(s) 1336, 1683, 2058
PpuMI RGGWCCY 2 cut(s) 514, 1996
PshBI ATTAAT 1 cut(s) 2141
Psp124BI GAGCTC 1 cut(s) 210
Psp5II RGGWCCY 2 cut(s) 514, 1996
Psp6I CCWGG 2 cut(s) 1102, 2070
PspGI CCWGG 2 cut(s) 1102, 2070
PspN4I GGNNCC 3 cut(s) 45, 264, 2069
PspPI GGNCC 3 cut(s) 514, 616, 1996
PspPPI RGGWCCY 2 cut(s) 514, 1996
PstI CTGCAG 2 cut(s) 271, 1297
PsuI RGATCY 4 cut(s) 194, 1635, 1657, 1686
RsaI GTAC 7 cut(s) 234, 246, 1080, 1341, 1978, 2399, 2479
RsaNI GTAC 7 cut(s) 233, 245, 1079, 1340, 1977, 2398, 2478
RseI CAYNNNNRTG 2 cut(s) 897, 1353
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 774
SatI GCNGC 7 cut(s) 147, 1010, 1296, 1924, 2234, 2305, 2413
Sau3AI GATC 9 cut(s) 124, 168, 194, 329, 481, 887, 1635, 1657, 1686
Sau96I GGNCC 3 cut(s) 514, 616, 1996
ScaI AGTACT 1 cut(s) 1080
SchI GAGTC 3 cut(s) 1337, 1684, 2059
ScrFI CCNGG 4 cut(s) 747, 1104, 2072, 2292
SduI GDGCHC 4 cut(s) 48, 210, 1407, 2625
SfaNI GCATC 3 cut(s) 754, 1158, 2074
SfcI CTRYAG 5 cut(s) 267, 1293, 1698, 2243, 2361
Sfr274I CTCGAG 1 cut(s) 1661
SinI GGWCC 3 cut(s) 514, 616, 1996
SlaI CTCGAG 1 cut(s) 1661
SmiI ATTTAAAT 1 cut(s) 957
SmiMI CAYNNNNRTG 2 cut(s) 897, 1353
SmlI CTYRAG 9 cut(s) 875, 1218, 1661, 1718, 1733, 1813, 2332, 2401, 2615
SmoI CTYRAG 9 cut(s) 875, 1218, 1661, 1718, 1733, 1813, 2332, 2401, 2615
SsiI CCGC 2 cut(s) 136, 2561
SspI AATATT 1 cut(s) 1021
SspMI CTAG 9 cut(s) 278, 308, 485, 693, 869, 1316, 1679, 2033, 2556
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 4 cut(s) 745, 1102, 2070, 2290
StyI CCWWGG 1 cut(s) 510
SwaI ATTTAAAT 1 cut(s) 957
TaaI ACNGT 3 cut(s) 942, 1344, 2677
TaiI ACGT 2 cut(s) 1237, 2138
TaqI TCGA 6 cut(s) 164, 1145, 1331, 1662, 2188, 2697
TatI WGTACW 3 cut(s) 1078, 2397, 2477
TfiI GAWTC 6 cut(s) 290, 311, 1729, 1852, 2128, 2524
TscAI CASTG 2 cut(s) 1349, 2490
TseFI GTSAC 1 cut(s) 1568
TseI GCWGC 7 cut(s) 146, 1009, 1295, 1923, 2233, 2304, 2412
Tsp45I GTSAC 1 cut(s) 1568
TspRI CASTG 2 cut(s) 1349, 2490
Vha464I CTTAAG 3 cut(s) 875, 1733, 2615
VpaK11BI GGWCC 3 cut(s) 514, 616, 1996
VspI ATTAAT 1 cut(s) 2141
XagI CCTNNNNNAGG 1 cut(s) 2226
XapI RAATTY 6 cut(s) 422, 1382, 1835, 2392, 2452, 2509
XbaI TCTAGA 1 cut(s) 2555
XceI RCATGY 2 cut(s) 1041, 1525
XhoI CTCGAG 1 cut(s) 1661
XmiI GTMKAC 1 cut(s) 416
XmnI GAANNNNTTC 3 cut(s) 289, 465, 2007
XspI CTAG 9 cut(s) 278, 308, 485, 693, 869, 1316, 1679, 2033, 2556
ZrmI AGTACT 1 cut(s) 1080
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.